MS012846 (gene) Bitter gourd (TR) v1

Overview


Name	MS012846
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNA polymerase II transcriptional coactivator KELP-like
Location	scaffold63: 3922059 .. 3923169 (+)
RNA-Seq Expression	MS012846
Synteny	MS012846

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCCGATATTCAACGTAGAATCAAGGAAACCGTCCGCAAAATTCTTGAATCTTCCGACATGGACGAGATGACCGAGTCCAAGATTCGAACCCTCGCCTCCAAAGAGCTCGATCTCGACCTCTCAAAATCGTCCTACAAGGCTCTGGTTCGCCAGGTCGTGGAATCCTTCCTCGAAGAACGAGCTGAAGAACAGCCGCAGGAGGAGCAAGAAGGTGCCCCTGCTAAGGAGAAGGAGTACGACGACGACGGCGATCTCGTTGTTTGTTGGGTTTGTTTTACCTAAACTCTTACTCTTCTCATGTTTAGTGGCTTGAGGTTTTGATTTCTTCGATTTGTGGTGAAAATTTGGCGATTAGAAGTTTTGATTGATGTGTTTGTGGTGGAATTTGAAAATATGGTGTTCGTGTTCCTTGACGCAGTTATCGAGCAAGAGGAAGGTGACGATTCAGGATTTTAGAGGGAAAACTCTGGTTTCGATTAGGGAGTTTTATAGGAAGGATGACAAAGATCTCCCTACGGCTAAAGGTGATTGTCCTAATGCTAATTGCTTGCTGATTATAAAATTTTGTATGTTAATATTACTTTCAGAGACTGTAATTTACGCAAAAGTCTTATTATATTTGTGCTTCTTGTTCGATATTGTGAAGATGATGTACATTCTACTGCTCCGTAATCACACCACACTCAACCATGCCATGATGCATTGAGAACCTTTACTAAATTGAATCCTACCAATGAAGCAAATGTTGGTGAATTAGAAGTAAGAACAACACAATTGTTGGACTAATGACATGGGGAAAGTGATAAGATAATAATCATTGAACATGAACGCTTTACTGAGGCTAAATGAGCAACCATGGCCACACATTTGATTTGTGAGGCTTGTATGAGTTTAATTCTTATAAAGCAAAAATTTCAGGGCGCATCACGGATTTGTTTTTTGATCAAACTTATATAATTTTTGCTTTCTGCATTGGAAATGGACTAATTATATTGGTATTATTTTTCCCTCAGGAATAAGCTTGACCGAGGAACAGTGGTCTGTCTTTAAAAAGAATGTACCCGACATAGAAAAAGCTATTAAGAATATGCAATCACGAATAATG

mRNA sequence

ATGGATGCCGATATTCAACGTAGAATCAAGGAAACCGTCCGCAAAATTCTTGAATCTTCCGACATGGACGAGATGACCGAGTCCAAGATTCGAACCCTCGCCTCCAAAGAGCTCGATCTCGACCTCTCAAAATCGTCCTACAAGGCTCTGGTTCGCCAGGTCGTGGAATCCTTCCTCGAAGAACGAGCTGAAGAACAGCCGCAGGAGGAGCAAGAAGGTGCCCCTGCTAAGGAGAAGGAGTACGACGACGACGGCGATCTCGTTGTTTGTTGGTTATCGAGCAAGAGGAAGGTGACGATTCAGGATTTTAGAGGGAAAACTCTGGTTTCGATTAGGGAGTTTTATAGGAAGGATGACAAAGATCTCCCTACGGCTAAAGGAATAAGCTTGACCGAGGAACAGTGGTCTGTCTTTAAAAAGAATGTACCCGACATAGAAAAAGCTATTAAGAATATGCAATCACGAATAATG

Coding sequence (CDS)

ATGGATGCCGATATTCAACGTAGAATCAAGGAAACCGTCCGCAAAATTCTTGAATCTTCCGACATGGACGAGATGACCGAGTCCAAGATTCGAACCCTCGCCTCCAAAGAGCTCGATCTCGACCTCTCAAAATCGTCCTACAAGGCTCTGGTTCGCCAGGTCGTGGAATCCTTCCTCGAAGAACGAGCTGAAGAACAGCCGCAGGAGGAGCAAGAAGGTGCCCCTGCTAAGGAGAAGGAGTACGACGACGACGGCGATCTCGTTGTTTGTTGGTTATCGAGCAAGAGGAAGGTGACGATTCAGGATTTTAGAGGGAAAACTCTGGTTTCGATTAGGGAGTTTTATAGGAAGGATGACAAAGATCTCCCTACGGCTAAAGGAATAAGCTTGACCGAGGAACAGTGGTCTGTCTTTAAAAAGAATGTACCCGACATAGAAAAAGCTATTAAGAATATGCAATCACGAATAATG

Protein sequence

MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLEERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM

Homology

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BLAST of MS012846 vs. NCBI nr
Match: XP_022136923.1 (RNA polymerase II transcriptional coactivator KELP-like [Momordica charantia])

HSP 1 Score: 293.5 bits (750), Expect = 1.1e-75
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MDADIQRRIKETVR ILESSDMDEMTESKIRTLASKELDLDLS+SSYKALVRQVVESFLE
Sbjct: 1   MDADIQRRIKETVRNILESSDMDEMTESKIRTLASKELDLDLSRSSYKALVRQVVESFLE 60

Query: 61  ERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDK 120
           ERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDK
Sbjct: 61  ERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDK 120

Query: 121 DLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           DLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM
Sbjct: 121 DLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 157

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BLAST of MS012846 vs. NCBI nr
Match: XP_022931551.1 (RNA polymerase II transcriptional coactivator KELP-like [Cucurbita moschata] >XP_023551821.1 RNA polymerase II transcriptional coactivator KELP-like [Cucurbita pepo subsp. pepo] >KAG6577134.1 RNA polymerase II transcriptional coactivator KELP, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015130.1 RNA polymerase II transcriptional coactivator KELP [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-64
Identity = 137/158 (86.71%), Postives = 148/158 (93.67%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD++IQRRI+E VR+ILESSDMDEMTESKIR LASK+LDLDLSKS YKALVR+VVESFL+
Sbjct: 1   MDSEIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           ER+E+QPQEE E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  ERSEDQPQEEAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFK NVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKNNVPAIEKAIKNMQSQIM 158

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BLAST of MS012846 vs. NCBI nr
Match: XP_008451928.1 (PREDICTED: RNA polymerase II transcriptional coactivator KELP [Cucumis melo] >KAA0044928.1 RNA polymerase II transcriptional coactivator KELP [Cucumis melo var. makuwa] >TYK16542.1 RNA polymerase II transcriptional coactivator KELP [Cucumis melo var. makuwa])

HSP 1 Score: 251.5 bits (641), Expect = 4.7e-63
Identity = 133/158 (84.18%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD++ QRRI+E VR+ILE+SDMDEMTESKIR LASK+LDLDLSKS YK+LVR VVESFL+
Sbjct: 1   MDSETQRRIEEAVRRILETSDMDEMTESKIRALASKDLDLDLSKSPYKSLVRTVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           ER+E+QPQ++ E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  ERSEQQPQDQAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFKKNVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMQSQIM 158

[Close]

BLAST of MS012846 vs. NCBI nr
Match: XP_038878439.1 (RNA polymerase II transcriptional coactivator KELP-like [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 4.7e-63
Identity = 133/156 (85.26%), Postives = 147/156 (94.23%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD++IQRRI+E VR+ILESSDMDEMTESKIR LASK+LDLDLSKS YKALVR+VVESFL+
Sbjct: 1   MDSEIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           ER+EEQPQ++ + A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  ERSEEQPQDQADDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSR 156
           KDLPTAKGISLTEEQWSVFKKNVP IEKAIKNM+S+
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMRSQ 156

[Close]

BLAST of MS012846 vs. NCBI nr
Match: XP_022985429.1 (RNA polymerase II transcriptional coactivator KELP-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 250.4 bits (638), Expect = 1.0e-62
Identity = 135/158 (85.44%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD+ IQRRI+E VR+ILESSDMDEMTESKIR LASK+LDLDLSKS YKALVR+VVESFL+
Sbjct: 1   MDSKIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           +R+E+Q QEE E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  KRSEDQLQEEAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFK NVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKNNVPAIEKAIKNMQSQIM 158

[Close]

BLAST of MS012846 vs. ExPASy Swiss-Prot
Match: O65155 (RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=3702 GN=KELP PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 8.1e-42
Identity = 94/165 (56.97%), Postives = 127/165 (76.97%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFL- 60
           M+ + + +I++TV +IL  SDM E+TE K+R LAS++L +DLS+ S+KA VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  EERAEEQP-----QEEQEG---APAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSI 120
           EERA E       +EE++G        KE+DDDGDL++C LS KR+VTIQ+F+GK+LVSI
Sbjct: 61  EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICRLSDKRRVTIQEFKGKSLVSI 120

Query: 121 REFYRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRI 157
           RE+Y+KD K+LPT+KGISLT+EQWS FKKN+P IE A+K M+SR+
Sbjct: 121 REYYKKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESRV 165

[Close]

BLAST of MS012846 vs. ExPASy Swiss-Prot
Match: O65154 (RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=3702 GN=KIWI PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 6.7e-12
Identity = 38/98 (38.78%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 60  EERAEEQPQEEQEGAPAKE-----KEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREF 119
           +E       E +  APAK+      + D   D+VVC +S  R+V+++++ GK  + IREF
Sbjct: 9   DEDVRASDDESETHAPAKKVAKPADDSDQSDDIVVCNISKNRRVSVRNWNGKIWIDIREF 68

Query: 120 YRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNM 153
           Y KD K LP  KGISL+ +QW+  + +  DIEKA+ ++
Sbjct: 69  YVKDGKTLPGKKGISLSVDQWNTLRNHAEDIEKALSDL 106

[Close]

BLAST of MS012846 vs. ExPASy Swiss-Prot
Match: Q9FHX8 (Mediator-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g42060 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-09
Identity = 34/61 (55.74%), Postives = 50/61 (81.97%), Query Frame = 0


Query: 1  MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
          +D D++R+IK+TV+KILESS++ ++TE K R  AS +LDLDLS+  YK +V++ VE+FLE
Sbjct: 11 IDKDLRRKIKKTVKKILESSNLYKITEIKAREEASLKLDLDLSQDPYKVIVKEEVENFLE 70

Query: 61 E 62
          E
Sbjct: 71 E 71

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BLAST of MS012846 vs. ExPASy Swiss-Prot
Match: P11031 (Activated RNA polymerase II transcriptional coactivator p15 OS=Mus musculus OX=10090 GN=Sub1 PE=1 SV=3)

HSP 1 Score: 54.3 bits (129), Expect = 1.4e-06
Identity = 36/125 (28.80%), Postives = 65/125 (52.00%), Query Frame = 0


Query: 29  KIRTLASKELDLDLSKSSYKALVRQVVESFLEERAEEQPQEEQEGAPAKEKEYDDDGDLV 88
           K + L S       S S  +  +++  ++  E+  ++Q   E   A A  K+     D  
Sbjct: 3   KSKELVSSSSSGSDSDSEVEKKLKRKKQAVPEKPVKKQKPGETSRALASSKQSSSSRDDN 62

Query: 89  VCWLSSKRKVTIQDFRGKTLVSIREFYRKDDKDL-PTAKGISLTEEQWSVFKKNVPDIEK 148
           +  +   R V+++DF+GK L+ IRE++   + ++ P  KGISL  EQWS  K+ + DI+ 
Sbjct: 63  MFQIGKMRYVSVRDFKGKILIDIREYWMDSEGEMKPGRKGISLNMEQWSQLKEQISDIDD 122

Query: 149 AIKNM 153
           A++ +
Sbjct: 123 AVRKL 127

[Close]

BLAST of MS012846 vs. ExPASy Swiss-Prot
Match: P53999 (Activated RNA polymerase II transcriptional coactivator p15 OS=Homo sapiens OX=9606 GN=SUB1 PE=1 SV=3)

HSP 1 Score: 53.5 bits (127), Expect = 2.5e-06
Identity = 30/94 (31.91%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 60  EERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 119
           E+  ++Q   E   A +  K+     D  +  +   R V+++DF+GK L+ IRE++   +
Sbjct: 34  EKPVKKQKTGETSRALSSSKQSSSSRDDNMFQIGKMRYVSVRDFKGKVLIDIREYWMDPE 93

Query: 120 KDL-PTAKGISLTEEQWSVFKKNVPDIEKAIKNM 153
            ++ P  KGISL  EQWS  K+ + DI+ A++ +
Sbjct: 94  GEMKPGRKGISLNPEQWSQLKEQISDIDDAVRKL 127

[Close]

BLAST of MS012846 vs. ExPASy TrEMBL
Match: A0A6J1C8V0 (RNA polymerase II transcriptional coactivator KELP-like OS=Momordica charantia OX=3673 GN=LOC111008499 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.2e-76
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MDADIQRRIKETVR ILESSDMDEMTESKIRTLASKELDLDLS+SSYKALVRQVVESFLE
Sbjct: 1   MDADIQRRIKETVRNILESSDMDEMTESKIRTLASKELDLDLSRSSYKALVRQVVESFLE 60

Query: 61  ERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDK 120
           ERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDK
Sbjct: 61  ERAEEQPQEEQEGAPAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDDK 120

Query: 121 DLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           DLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM
Sbjct: 121 DLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 157

[Close]

BLAST of MS012846 vs. ExPASy TrEMBL
Match: A0A6J1ETY4 (RNA polymerase II transcriptional coactivator KELP-like OS=Cucurbita moschata OX=3662 GN=LOC111437695 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.4e-65
Identity = 137/158 (86.71%), Postives = 148/158 (93.67%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD++IQRRI+E VR+ILESSDMDEMTESKIR LASK+LDLDLSKS YKALVR+VVESFL+
Sbjct: 1   MDSEIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           ER+E+QPQEE E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  ERSEDQPQEEAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFK NVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKNNVPAIEKAIKNMQSQIM 158

[Close]

BLAST of MS012846 vs. ExPASy TrEMBL
Match: A0A5A7TPV6 (RNA polymerase II transcriptional coactivator KELP OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G003580 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.3e-63
Identity = 133/158 (84.18%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD++ QRRI+E VR+ILE+SDMDEMTESKIR LASK+LDLDLSKS YK+LVR VVESFL+
Sbjct: 1   MDSETQRRIEEAVRRILETSDMDEMTESKIRALASKDLDLDLSKSPYKSLVRTVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           ER+E+QPQ++ E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  ERSEQQPQDQAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFKKNVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMQSQIM 158

[Close]

BLAST of MS012846 vs. ExPASy TrEMBL
Match: A0A1S3BS20 (RNA polymerase II transcriptional coactivator KELP OS=Cucumis melo OX=3656 GN=LOC103493081 PE=3 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.3e-63
Identity = 133/158 (84.18%), Postives = 147/158 (93.04%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD++ QRRI+E VR+ILE+SDMDEMTESKIR LASK+LDLDLSKS YK+LVR VVESFL+
Sbjct: 1   MDSETQRRIEEAVRRILETSDMDEMTESKIRALASKDLDLDLSKSPYKSLVRTVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           ER+E+QPQ++ E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  ERSEQQPQDQAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFKKNVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKKNVPAIEKAIKNMQSQIM 158

[Close]

BLAST of MS012846 vs. ExPASy TrEMBL
Match: A0A6J1JD91 (RNA polymerase II transcriptional coactivator KELP-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111483435 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 5.1e-63
Identity = 135/158 (85.44%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           MD+ IQRRI+E VR+ILESSDMDEMTESKIR LASK+LDLDLSKS YKALVR+VVESFL+
Sbjct: 1   MDSKIQRRIEEAVRRILESSDMDEMTESKIRALASKDLDLDLSKSPYKALVRKVVESFLQ 60

Query: 61  ERAEEQPQEEQEGA-PAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREFYRKDD 120
           +R+E+Q QEE E A  AKEKEYDDDGDL+VCWLSSKRKVTIQDFRGKTLVSIREFYRKD 
Sbjct: 61  KRSEDQLQEEAEDASAAKEKEYDDDGDLIVCWLSSKRKVTIQDFRGKTLVSIREFYRKDG 120

Query: 121 KDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRIM 158
           KDLPTAKGISLTEEQWSVFK NVP IEKAIKNMQS+IM
Sbjct: 121 KDLPTAKGISLTEEQWSVFKNNVPAIEKAIKNMQSQIM 158

[Close]

BLAST of MS012846 vs. TAIR 10
Match: AT4G10920.1 (transcriptional coactivator p15 (PC4) family protein (KELP) )

HSP 1 Score: 171.4 bits (433), Expect = 5.8e-43
Identity = 94/165 (56.97%), Postives = 127/165 (76.97%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFL- 60
           M+ + + +I++TV +IL  SDM E+TE K+R LAS++L +DLS+ S+KA VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  EERAEEQP-----QEEQEG---APAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSI 120
           EERA E       +EE++G        KE+DDDGDL++C LS KR+VTIQ+F+GK+LVSI
Sbjct: 61  EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICRLSDKRRVTIQEFKGKSLVSI 120

Query: 121 REFYRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRI 157
           RE+Y+KD K+LPT+KGISLT+EQWS FKKN+P IE A+K M+SR+
Sbjct: 121 REYYKKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESRV 165

[Close]

BLAST of MS012846 vs. TAIR 10
Match: AT4G10920.2 (transcriptional coactivator p15 (PC4) family protein (KELP) )

HSP 1 Score: 171.4 bits (433), Expect = 5.8e-43
Identity = 94/165 (56.97%), Postives = 127/165 (76.97%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFL- 60
           M+ + + +I++TV +IL  SDM E+TE K+R LAS++L +DLS+ S+KA VR VVE FL 
Sbjct: 1   MEKETKEKIEKTVIEILSESDMKEITEFKVRKLASEKLAIDLSEKSHKAFVRSVVEKFLD 60

Query: 61  EERAEEQP-----QEEQEG---APAKEKEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSI 120
           EERA E       +EE++G        KE+DDDGDL++C LS KR+VTIQ+F+GK+LVSI
Sbjct: 61  EERAREYENSQVNKEEEDGDKDCGKGNKEFDDDGDLIICRLSDKRRVTIQEFKGKSLVSI 120

Query: 121 REFYRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRI 157
           RE+Y+KD K+LPT+KGISLT+EQWS FKKN+P IE A+K M+SR+
Sbjct: 121 REYYKKDGKELPTSKGISLTDEQWSTFKKNMPAIENAVKKMESRV 165

[Close]

BLAST of MS012846 vs. TAIR 10
Match: AT4G00980.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 81.6 bits (200), Expect = 6.0e-16
Identity = 61/163 (37.42%), Postives = 88/163 (53.99%), Query Frame = 0


Query: 1   MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
           M+    ++I+ETV+ IL  SDMD+MTE K+R  AS +L +DLS +++K LVR V+E FL 
Sbjct: 12  MEIVATQKIEETVKSILSESDMDQMTEFKLRLDASAKLGIDLSGTNHKKLVRDVLEVFLL 71

Query: 61  ERAEEQPQEEQEGAPAKEK-------EYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIRE 120
               E    E   APAK +           + +  +C LS K+  T+Q +RG+  +SI  
Sbjct: 72  STPGEALVPETV-APAKNETVSVAAASVGGEDERFICKLSEKQNATVQRYRGQPFLSI-- 131

Query: 121 FYRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNMQSRI 157
                 +     +G  L+  QWSV KKN   IE  IK  QS++
Sbjct: 132 ---GSQEHGKAFRGAHLSTNQWSVIKKNFAAIEDGIKQCQSKL 168

[Close]

BLAST of MS012846 vs. TAIR 10
Match: AT5G09250.1 (ssDNA-binding transcriptional regulator )

HSP 1 Score: 72.0 bits (175), Expect = 4.8e-13
Identity = 38/98 (38.78%), Postives = 59/98 (60.20%), Query Frame = 0


Query: 60  EERAEEQPQEEQEGAPAKE-----KEYDDDGDLVVCWLSSKRKVTIQDFRGKTLVSIREF 119
           +E       E +  APAK+      + D   D+VVC +S  R+V+++++ GK  + IREF
Sbjct: 9   DEDVRASDDESETHAPAKKVAKPADDSDQSDDIVVCNISKNRRVSVRNWNGKIWIDIREF 68

Query: 120 YRKDDKDLPTAKGISLTEEQWSVFKKNVPDIEKAIKNM 153
           Y KD K LP  KGISL+ +QW+  + +  DIEKA+ ++
Sbjct: 69  YVKDGKTLPGKKGISLSVDQWNTLRNHAEDIEKALSDL 106

[Close]

BLAST of MS012846 vs. TAIR 10
Match: AT5G42060.1 (DEK, chromatin associated protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-10
Identity = 34/61 (55.74%), Postives = 50/61 (81.97%), Query Frame = 0


Query: 1  MDADIQRRIKETVRKILESSDMDEMTESKIRTLASKELDLDLSKSSYKALVRQVVESFLE 60
          +D D++R+IK+TV+KILESS++ ++TE K R  AS +LDLDLS+  YK +V++ VE+FLE
Sbjct: 11 IDKDLRRKIKKTVKKILESSNLYKITEIKAREEASLKLDLDLSQDPYKVIVKEEVENFLE 70

Query: 61 E 62
          E
Sbjct: 71 E 71

The following BLAST results are available for this feature:

BLAST of MS012846 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136923.1	1.1e-75	98.73	RNA polymerase II transcriptional coactivator KELP-like [Momordica charantia]	[more]
XP_022931551.1	1.1e-64	86.71	RNA polymerase II transcriptional coactivator KELP-like [Cucurbita moschata] >XP...	[more]
XP_008451928.1	4.7e-63	84.18	PREDICTED: RNA polymerase II transcriptional coactivator KELP [Cucumis melo] >KA...	[more]
XP_038878439.1	4.7e-63	85.26	RNA polymerase II transcriptional coactivator KELP-like [Benincasa hispida]	[more]
XP_022985429.1	1.0e-62	85.44	RNA polymerase II transcriptional coactivator KELP-like isoform X3 [Cucurbita ma...	[more]

BLAST of MS012846 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65155	8.1e-42	56.97	RNA polymerase II transcriptional coactivator KELP OS=Arabidopsis thaliana OX=37...	[more]
O65154	6.7e-12	38.78	RNA polymerase II transcriptional coactivator KIWI OS=Arabidopsis thaliana OX=37...	[more]
Q9FHX8	1.8e-09	55.74	Mediator-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g42060 PE=1 ...	[more]
P11031	1.4e-06	28.80	Activated RNA polymerase II transcriptional coactivator p15 OS=Mus musculus OX=1...	[more]
P53999	2.5e-06	31.91	Activated RNA polymerase II transcriptional coactivator p15 OS=Homo sapiens OX=9...	[more]

BLAST of MS012846 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8V0	5.2e-76	98.73	RNA polymerase II transcriptional coactivator KELP-like OS=Momordica charantia O...	[more]
A0A6J1ETY4	5.4e-65	86.71	RNA polymerase II transcriptional coactivator KELP-like OS=Cucurbita moschata OX...	[more]
A0A5A7TPV6	2.3e-63	84.18	RNA polymerase II transcriptional coactivator KELP OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BS20	2.3e-63	84.18	RNA polymerase II transcriptional coactivator KELP OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1JD91	5.1e-63	85.44	RNA polymerase II transcriptional coactivator KELP-like isoform X3 OS=Cucurbita ...	[more]

BLAST of MS012846 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10920.1	5.8e-43	56.97	transcriptional coactivator p15 (PC4) family protein (KELP)	[more]
AT4G10920.2	5.8e-43	56.97	transcriptional coactivator p15 (PC4) family protein (KELP)	[more]
AT4G00980.1	6.0e-16	37.42	zinc knuckle (CCHC-type) family protein	[more]
AT5G09250.1	4.8e-13	38.78	ssDNA-binding transcriptional regulator	[more]
AT5G42060.1	1.3e-10	55.74	DEK, chromatin associated protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017415	Putative RNA polymerase II transcriptional coactivator KELP	PIRSF	PIRSF038156	RNA_polymII_KELP	coord: 1..156 e-value: 4.5E-53 score: 177.4
IPR003173	Transcriptional coactivator p15 (PC4), C-terminal	PFAM	PF02229	PC4	coord: 92..140 e-value: 8.4E-21 score: 73.4
IPR009044	ssDNA-binding transcriptional regulator	GENE3D	2.30.31.10	Transcriptional Coactivator Pc4; Chain A	coord: 79..157 e-value: 3.7E-26 score: 92.5
IPR009044	ssDNA-binding transcriptional regulator	SUPERFAMILY	54447	ssDNA-binding transcriptional regulator domain	coord: 89..151
IPR014876	DEK, C-terminal	PFAM	PF08766	DEK_C	coord: 8..59 e-value: 4.9E-12 score: 45.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..81
None	No IPR available	PANTHER	PTHR13215:SF6	RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR KELP	coord: 4..155
IPR045125	RNA polymerase II transcriptional coactivator Sub1/Tcp4-like	PANTHER	PTHR13215	RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR	coord: 4..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012846.1	MS012846.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060261	positive regulation of transcription initiation from RNA polymerase II promoter
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003713	transcription coactivator activity