MS012842 (gene) Bitter gourd (TR) v1

BLAST of MS012842 vs. NCBI nr
Match: XP_022136745.1 (GDSL esterase/lipase At1g71691 [Momordica charantia])

HSP 1 Score: 809.7 bits (2090), Expect = 1.1e-230
Identity = 392/395 (99.24%), Postives = 394/395 (99.75%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNLIIPLISITISTIFLQSQALLNPKDH+PPAPSP+GFISPPN SSPISPVAPPPSNS
Sbjct: 1   MALNLIIPLISITISTIFLQSQALLNPKDHHPPAPSPYGFISPPNASSPISPVAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG
Sbjct: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL
Sbjct: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP
Sbjct: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW
Sbjct: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL
Sbjct: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 395

BLAST of MS012842 vs. NCBI nr
Match: XP_038904573.1 (GDSL esterase/lipase At1g71691 [Benincasa hispida])

HSP 1 Score: 747.7 bits (1929), Expect = 5.3e-212
Identity = 359/396 (90.66%), Postives = 378/396 (95.45%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPK-DHYPPAPSPHGFISPPNESSPISPVAPPPSN 60
           MAL+L+I LI I ISTIFLQS ALLNP+    P AP+P+ F +PPN S PISP+APPPSN
Sbjct: 1   MALDLLISLIFILISTIFLQSTALLNPRAPPNPLAPTPYAFTTPPNSSFPISPLAPPPSN 60

Query: 61  SLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRL 120
           SLVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH+PTGRFCNGRIPVDFLALRL
Sbjct: 61  SLVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHTPTGRFCNGRIPVDFLALRL 120

Query: 121 GLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFV 180
           GLPFVPSYLGH+GAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFV
Sbjct: 121 GLPFVPSYLGHSGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFV 180

Query: 181 LSMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKN 240
           L+MGE  AADHISNSVFYISIGINDYIHYYLFNIS+VQNLY PWNFNQFLAATIRQEIKN
Sbjct: 181 LNMGENAAADHISNSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAATIRQEIKN 240

Query: 241 LYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMEL 300
           LYNMNARRIV+MGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMEL
Sbjct: 241 LYNMNARRIVVMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMEL 300

Query: 301 PDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIW 360
           PDSSIIFCDLL+GSMDILKNHERYGFNVT+DACCGFGRYNGWIMCISP+MACKNASNHIW
Sbjct: 301 PDSSIIFCDLLQGSMDILKNHERYGFNVTSDACCGFGRYNGWIMCISPIMACKNASNHIW 360

Query: 361 WDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           WDQFHPTDAVN ILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 WDQFHPTDAVNGILADNVWNGLHTTMCYPKNLQDVI 396

BLAST of MS012842 vs. NCBI nr
Match: XP_023552224.1 (GDSL esterase/lipase At1g71691 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 733.4 bits (1892), Expect = 1.0e-207
Identity = 351/395 (88.86%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNL+I LI I IS+IFL S AL+ PK H P AP+P+GF +PPN S P  P APPPSNS
Sbjct: 1   MALNLLIFLIFILISSIFLPSTALIIPKLHNPLAPTPYGFTTPPNSSFPTFPAAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RLG
Sbjct: 61  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFT+QIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTRQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           +MGE  AADHIS+SVFYISIGINDYIHYYLFNIS VQNLY PWNFNQFLA TI+QEIKNL
Sbjct: 181 TMGENAAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIV+MGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRY+VEELGMELP
Sbjct: 241 YNMNARRIVVMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYIVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           D SIIFCDLL+GSMDILKNHERYGFNVT+DACCGFGRYNGWI+CISP+MACKNASNHIWW
Sbjct: 301 DLSIIFCDLLQGSMDILKNHERYGFNVTSDACCGFGRYNGWILCISPLMACKNASNHIWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 DQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 395

BLAST of MS012842 vs. NCBI nr
Match: XP_022931385.1 (GDSL esterase/lipase At1g71691 [Cucurbita moschata] >KAG7015133.1 GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 732.3 bits (1889), Expect = 2.3e-207
Identity = 350/395 (88.61%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNL+I LI I IS+IFL S AL+ PK H P AP+P+GF +PPN S P  P APPPSNS
Sbjct: 1   MALNLLIFLIFILISSIFLPSTALIIPKLHNPLAPTPYGFTTPPNSSFPTFPAAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RLG
Sbjct: 61  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFT+QIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTRQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           +MGE  AADHIS+SVFYISIGINDYIHYYLFNIS VQNLY PWNFNQFLA TI+QEIKNL
Sbjct: 181 TMGENAAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIV+MGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRY+VEELGMELP
Sbjct: 241 YNMNARRIVVMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYIVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           D SIIFCDLL+GSMDILKNHERYGFNVT+DACCGFGRYNGWI+CISP+MAC+NASNHIWW
Sbjct: 301 DLSIIFCDLLQGSMDILKNHERYGFNVTSDACCGFGRYNGWILCISPLMACRNASNHIWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 DQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 395

BLAST of MS012842 vs. NCBI nr
Match: XP_022985405.1 (GDSL esterase/lipase At1g71691 [Cucurbita maxima])

HSP 1 Score: 730.7 bits (1885), Expect = 6.6e-207
Identity = 350/395 (88.61%), Postives = 369/395 (93.42%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNL+I LI I ISTIFL S AL+ PK H P AP+P+GF SPPN S P    APPPSNS
Sbjct: 1   MALNLLIFLIFILISTIFLPSTALITPKLHNPLAPTPYGFTSPPNSSFPTLRAAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RLG
Sbjct: 61  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFT+QIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTRQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           +MGE  AADHIS+SVFYISIGINDYIHYYLFNIS VQNLY PWNFNQFLA TI+QEIKNL
Sbjct: 181 TMGENAAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIV+MGLAPIGCAPFYLWQYGS NGECIEEINDMVMEFNFAMRY+VEELGMELP
Sbjct: 241 YNMNARRIVVMGLAPIGCAPFYLWQYGSVNGECIEEINDMVMEFNFAMRYIVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           D SIIFCDLL+GSMDILKNHERYGFNVT+DACCGFGRYNGWI+CISP+MACKNASNH+WW
Sbjct: 301 DLSIIFCDLLQGSMDILKNHERYGFNVTSDACCGFGRYNGWILCISPLMACKNASNHVWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 DQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 395

BLAST of MS012842 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.1e-66
Identity = 137/354 (38.70%), Postives = 197/354 (55.65%), Query Frame = 0

Query: 49  PISPVAPPP-------SNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSP 108
           PIS  APPP        + +VPALFV GDS +D G NN + +FA+A++ PYG DF+   P
Sbjct: 35  PIS--APPPPLVDLNTGDGIVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFN-GGP 94

Query: 109 TGRFCNGRIPVDFLALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQH 168
           TGRFCNG   VD +A  LGLP +P+Y    G  + +++GVNYASA+AG++  +G      
Sbjct: 95  TGRFCNGLTMVDGIAQLLGLPLIPAYSEATG--DQVLRGVNYASAAAGILPDTGGNFVGR 154

Query: 169 ISFTQQIQQFMDTFQQFVLSMGEKNA-ADHISNSVFYISIGINDYIHYYLFNISSVQNLY 228
           I F QQI  F  T  Q     G   A AD ++ S+F+I +G NDY++ YL      +N Y
Sbjct: 155 IPFDQQIHNFETTLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQY 214

Query: 229 SPWNFNQFLAATIRQEIKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDM 288
           +   F   L      ++  LYN+  R+ V+ GL  +GC P  L Q    +G+C EE+N +
Sbjct: 215 NSQQFGDLLVQHYTDQLTRLYNLGGRKFVVAGLGRMGCIPSILAQ--GNDGKCSEEVNQL 274

Query: 289 VMEFNFAMRYMVEELGMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNG 348
           V+ FN  ++ M+  L   LPD+  I+ D+     DI+ N   YG       CCG G+  G
Sbjct: 275 VLPFNTNVKTMISNLNQNLPDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRG 334

Query: 349 WIMCISPVMACKNASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
            I C+     C N   +++WD FHPT+ VN I+A+  + G   T+ YP N++ +
Sbjct: 335 QITCLPFETPCPNRDQYVFWDAFHPTEKVNLIMAKKAFAG-DRTVAYPINIQQL 380

BLAST of MS012842 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.3e-64
Identity = 125/341 (36.66%), Postives = 193/341 (56.60%), Query Frame = 0

Query: 58  SNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLAL 117
           + ++ PA+FV GDS VD G NN L + AR+++LPYG DF  + PTGRF NG+  VDF+  
Sbjct: 43  TTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGE 102

Query: 118 RLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQ 177
            LGLP +P+++       D++ GVNYASA+ G++  +G  LG+  S  +Q++ F  T  +
Sbjct: 103 LLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLME 162

Query: 178 FVLSMGEKNAADHISNSVFYISIGINDYIHYYLF-NISSVQNLYSPWNFNQFLAATIRQE 237
              SM +++  ++++ S+  +S+G NDYI+ YL   +    ++Y P +F   L +     
Sbjct: 163 ISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTH 222

Query: 238 IKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELG 297
           +  LY    R+ VI G+ P+GC P  L    +  GEC+E +N+M   FN  +  +V+ L 
Sbjct: 223 LLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLN 282

Query: 298 MELPDSS---IIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKN 357
            +   +S    ++ +    ++DIL N   YGF VT   CCG GR  G I C+   + C  
Sbjct: 283 SDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCAF 342

Query: 358 ASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
              H++WD FHPT A N I+A   +NG  +  CYP NL  +
Sbjct: 343 RDRHVFWDAFHPTQAFNLIIALRAFNGSKSD-CYPINLSQL 382

BLAST of MS012842 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 7.2e-63
Identity = 129/324 (39.81%), Postives = 188/324 (58.02%), Query Frame = 0

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           L PA FV GDS VD+G NN++ T ARA++ PYG DF    PTGRFCNGR  VD+ A  LG
Sbjct: 27  LAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFG--FPTGRFCNGRTVVDYGATYLG 86

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LP VP YL      ++ ++GVNYASA+AG++  +G   G   +F  QI QF  T  +  L
Sbjct: 87  LPLVPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITI-ELRL 146

Query: 181 SMGEKNAAD---HISNSVFYISIGINDYIHYYLF-NISSVQNLYSPWNFNQFLAATIRQE 240
               +N AD   +++ S+  I+IG NDYI+ YL     S    YS  ++   L  T+  +
Sbjct: 147 RRFFQNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQ 206

Query: 241 IKNLYNMNARRIVIMGLAPIGCAPFYLWQY-GSENGECIEEINDMVMEFNFAMRYMVEEL 300
           I  LYN+ AR++V+ G  P+GC P  L    G+    C+ +IN+MV  FN  ++ +   L
Sbjct: 207 ISRLYNLGARKMVLAGSGPLGCIPSQLSMVTGNNTSGCVTKINNMVSMFNSRLKDLANTL 266

Query: 301 GMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNAS 360
              LP S  ++ ++ +   D++ N  RYG  V+ +ACCG GRY G + C+     C + +
Sbjct: 267 NTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDRN 326

Query: 361 NHIWWDQFHPTDAVNAILAENVWN 380
            +++WD FHPT+  N I+A N ++
Sbjct: 327 QYVFWDAFHPTETANKIIAHNTFS 347

BLAST of MS012842 vs. ExPASy Swiss-Prot
Match: Q9C7N5 (GDSL esterase/lipase At1g29660 OS=Arabidopsis thaliana OX=3702 GN=At1g29660 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 5.2e-61
Identity = 123/336 (36.61%), Postives = 189/336 (56.25%), Query Frame = 0

Query: 62  VPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLGL 121
           VP  F+ GDS VD G NN L + ARAD+ PYG DF    PTGRF NGR  VD L   LG 
Sbjct: 30  VPCYFIFGDSLVDNGNNNRLRSIARADYFPYGIDFG--GPTGRFSNGRTTVDVLTELLGF 89

Query: 122 P-FVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 181
             ++P+Y   +G  ++++QGVNYASA+AG+   +G++LGQ I+F+ Q++ + +T  Q V 
Sbjct: 90  DNYIPAYSTVSG--QEILQGVNYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVE 149

Query: 182 SMG-EKNAADHISNSVFYISIGINDYI-HYYLFNISSVQNLYSPWNFNQFLAATIRQEIK 241
            +G E  AAD++   ++ + +G NDY+ +Y++    S    Y+P  +   L +  R ++ 
Sbjct: 150 ILGDEYTAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLN 209

Query: 242 NLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGME 301
            LYN  AR+  ++G+  IGC+P  L Q   +   C+E IN     FN  +  MV++L   
Sbjct: 210 ALYNYGARKFALVGIGAIGCSPNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNA 269

Query: 302 LPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHI 361
             D+S  + +      DI+ N   YGF  T  ACCG GR  G + C+     C N   ++
Sbjct: 270 HSDASFTYINAYGAFQDIIANPSAYGFTNTNTACCGIGRNGGQLTCLPGEPPCLNRDEYV 329

Query: 362 WWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
           +WD FHP+ A N  +A+  +N   ++  YP ++  +
Sbjct: 330 FWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDISQL 361

BLAST of MS012842 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 9.7e-60
Identity = 123/334 (36.83%), Postives = 184/334 (55.09%), Query Frame = 0

Query: 62  VPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLGL 121
           VPA+FV+GDS VDAG NNFL T ARA+ LPYG D + + PTGRF NG   +D LA  L +
Sbjct: 39  VPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMN-YQPTGRFSNGLTFIDLLARLLEI 98

Query: 122 PFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVLS 181
           P  P +     +   ++QGVNYASA+AG++  SG   G   S  QQ+     T  Q    
Sbjct: 99  PSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTM 158

Query: 182 MGEKNAADHISNSVFYISIGINDYIHYYLF-NISSVQNLYSPWNFNQFLAATIRQEIKNL 241
           M  +N  D+++ S+  +  G NDYI+ YL  N+      + P +F   L +   +++  L
Sbjct: 159 MSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTL 218

Query: 242 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 301
           Y++  R+I I G+AP+GC P    +  S    C++ +N ++  FN  ++ +V++L    P
Sbjct: 219 YSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQRSP 278

Query: 302 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 361
            +  ++ +      DIL N   YGF+V   ACCG GR  G I C+     C N + +++W
Sbjct: 279 GAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNRNQYVFW 338

Query: 362 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
           D FHPT   N+ILA   + G   +  YP N++ +
Sbjct: 339 DAFHPTQTANSILARRAFYG-PPSDAYPVNVQQM 370

BLAST of MS012842 vs. ExPASy TrEMBL
Match: A0A6J1C4U4 (GDSL esterase/lipase At1g71691 OS=Momordica charantia OX=3673 GN=LOC111008376 PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 5.5e-231
Identity = 392/395 (99.24%), Postives = 394/395 (99.75%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNLIIPLISITISTIFLQSQALLNPKDH+PPAPSP+GFISPPN SSPISPVAPPPSNS
Sbjct: 1   MALNLIIPLISITISTIFLQSQALLNPKDHHPPAPSPYGFISPPNASSPISPVAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG
Sbjct: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL
Sbjct: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP
Sbjct: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW
Sbjct: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL
Sbjct: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 395

BLAST of MS012842 vs. ExPASy TrEMBL
Match: A0A6J1EZA3 (GDSL esterase/lipase At1g71691 OS=Cucurbita moschata OX=3662 GN=LOC111437583 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.1e-207
Identity = 350/395 (88.61%), Postives = 371/395 (93.92%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNL+I LI I IS+IFL S AL+ PK H P AP+P+GF +PPN S P  P APPPSNS
Sbjct: 1   MALNLLIFLIFILISSIFLPSTALIIPKLHNPLAPTPYGFTTPPNSSFPTFPAAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RLG
Sbjct: 61  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFT+QIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTRQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           +MGE  AADHIS+SVFYISIGINDYIHYYLFNIS VQNLY PWNFNQFLA TI+QEIKNL
Sbjct: 181 TMGENAAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIV+MGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRY+VEELGMELP
Sbjct: 241 YNMNARRIVVMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYIVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           D SIIFCDLL+GSMDILKNHERYGFNVT+DACCGFGRYNGWI+CISP+MAC+NASNHIWW
Sbjct: 301 DLSIIFCDLLQGSMDILKNHERYGFNVTSDACCGFGRYNGWILCISPLMACRNASNHIWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 DQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 395

BLAST of MS012842 vs. ExPASy TrEMBL
Match: A0A6J1JB87 (GDSL esterase/lipase At1g71691 OS=Cucurbita maxima OX=3661 GN=LOC111483416 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.2e-207
Identity = 350/395 (88.61%), Postives = 369/395 (93.42%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYPPAPSPHGFISPPNESSPISPVAPPPSNS 60
           MALNL+I LI I ISTIFL S AL+ PK H P AP+P+GF SPPN S P    APPPSNS
Sbjct: 1   MALNLLIFLIFILISTIFLPSTALITPKLHNPLAPTPYGFTSPPNSSFPTLRAAPPPSNS 60

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           LVPALFVIGDSTVD GTNNFLGTFARADHLPYGRDFDTH PTGRFCNGRIPVDFLA RLG
Sbjct: 61  LVPALFVIGDSTVDCGTNNFLGTFARADHLPYGRDFDTHRPTGRFCNGRIPVDFLAQRLG 120

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFT+QIQQFMDTFQQFVL
Sbjct: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTRQIQQFMDTFQQFVL 180

Query: 181 SMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQEIKNL 240
           +MGE  AADHIS+SVFYISIGINDYIHYYLFNIS VQNLY PWNFNQFLA TI+QEIKNL
Sbjct: 181 TMGENAAADHISDSVFYISIGINDYIHYYLFNISRVQNLYPPWNFNQFLATTIKQEIKNL 240

Query: 241 YNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGMELP 300
           YNMNARRIV+MGLAPIGCAPFYLWQYGS NGECIEEINDMVMEFNFAMRY+VEELGMELP
Sbjct: 241 YNMNARRIVVMGLAPIGCAPFYLWQYGSVNGECIEEINDMVMEFNFAMRYIVEELGMELP 300

Query: 301 DSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHIWW 360
           D SIIFCDLL+GSMDILKNHERYGFNVT+DACCGFGRYNGWI+CISP+MACKNASNH+WW
Sbjct: 301 DLSIIFCDLLQGSMDILKNHERYGFNVTSDACCGFGRYNGWILCISPLMACKNASNHVWW 360

Query: 361 DQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           DQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 DQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 395

BLAST of MS012842 vs. ExPASy TrEMBL
Match: A0A0A0L7F0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G270280 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.5e-201
Identity = 347/400 (86.75%), Postives = 370/400 (92.50%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQA-LLNPKDH---YPPAPSPHGFISPPNESSPI-SPVAP 60
           MAL+L+I L  I IS++FLQS A L NP  H    PP P+ +GF + PN S PI  P+ P
Sbjct: 1   MALSLLISLFFILISSVFLQSTAPLPNPAPHPNPLPPTPN-YGFTTIPNSSFPIPPPLPP 60

Query: 61  PPSNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFL 120
           PPSNSLVPALFVIGDSTVD+GTNNFLGTFARADHLPYGRDFDTH+PTGRF NGRIPVDFL
Sbjct: 61  PPSNSLVPALFVIGDSTVDSGTNNFLGTFARADHLPYGRDFDTHTPTGRFSNGRIPVDFL 120

Query: 121 ALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTF 180
           ALRLGLPFVPSYLGH GAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTF
Sbjct: 121 ALRLGLPFVPSYLGHVGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTF 180

Query: 181 QQFVLSMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIRQ 240
           QQFVL+MGEK AADHISNSVFYISIGINDYIHYYLFNIS+VQNLY PWNFNQFLA TIRQ
Sbjct: 181 QQFVLNMGEKAAADHISNSVFYISIGINDYIHYYLFNISNVQNLYPPWNFNQFLAVTIRQ 240

Query: 241 EIKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEEL 300
           EIKNLYNMNARRIV+MGLAPIGCAPFYLWQY SENG CIEEINDMVMEFNFAMRY+VEEL
Sbjct: 241 EIKNLYNMNARRIVVMGLAPIGCAPFYLWQYRSENGACIEEINDMVMEFNFAMRYVVEEL 300

Query: 301 GMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNAS 360
           GMELPDS+IIFCDLL+GSMDILKNHE YGFNVT++ACCGFGRYNGWIMCISP+MACKNAS
Sbjct: 301 GMELPDSNIIFCDLLQGSMDILKNHEYYGFNVTSNACCGFGRYNGWIMCISPIMACKNAS 360

Query: 361 NHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           NHIWWDQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 NHIWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 399

BLAST of MS012842 vs. ExPASy TrEMBL
Match: A0A5A7UZU1 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold420G00560 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 7.7e-201
Identity = 347/401 (86.53%), Postives = 367/401 (91.52%), Query Frame = 0

Query: 1   MALNLIIPLISITISTIFLQSQALLNPKDHYP----PAPSPHGFISPPNESSPI--SPVA 60
           MAL+L I L  I IS++FLQS   L     +P    P+    GF + PN S PI  SP+ 
Sbjct: 1   MALSLPISLFFILISSVFLQSATPLPNSAPHPNPLAPSTPNDGFTTIPNSSFPIPPSPLP 60

Query: 61  PPPSNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDF 120
           PPPSNSLVPALFVIGDSTVD+GTNNFLGTFARADHLPYGRDFDTH+PTGRFCNGRIPVDF
Sbjct: 61  PPPSNSLVPALFVIGDSTVDSGTNNFLGTFARADHLPYGRDFDTHTPTGRFCNGRIPVDF 120

Query: 121 LALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDT 180
           LALRLGLPFVPSYLGH GAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDT
Sbjct: 121 LALRLGLPFVPSYLGHVGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDT 180

Query: 181 FQQFVLSMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSPWNFNQFLAATIR 240
           FQQFVL+MGEK AADHISNSVFYISIGINDYIHYYLFNIS+VQNL+ PWNFNQFLAATIR
Sbjct: 181 FQQFVLNMGEKAAADHISNSVFYISIGINDYIHYYLFNISNVQNLFPPWNFNQFLAATIR 240

Query: 241 QEIKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEE 300
           QEIKNLYNMNARRIV+MGLAPIGCAPFYLWQY SENG CIEEINDMVMEFNFAMRYMVEE
Sbjct: 241 QEIKNLYNMNARRIVVMGLAPIGCAPFYLWQYRSENGACIEEINDMVMEFNFAMRYMVEE 300

Query: 301 LGMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNA 360
           LGMELPDSSIIFCDLLEGSMDILKNHE YGFNVT++ACCGFGRYNGWIMCISP+MACKNA
Sbjct: 301 LGMELPDSSIIFCDLLEGSMDILKNHEYYGFNVTSNACCGFGRYNGWIMCISPIMACKNA 360

Query: 361 SNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
           SNHIWWDQFHPTDAVNAILA+NVWNGLHTTMCYPKNL+D++
Sbjct: 361 SNHIWWDQFHPTDAVNAILADNVWNGLHTTMCYPKNLQDVI 401

BLAST of MS012842 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 534.3 bits (1375), Expect = 8.5e-152
Identity = 242/353 (68.56%), Postives = 288/353 (81.59%), Query Frame = 0

Query: 43  PPNESSPISPVAPPPSNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPT 102
           PP+ S         PS   VPALFV GDS+VD+GTNNFLGT ARAD LPYGRDFDTH PT
Sbjct: 50  PPSPSRRAQSPTVKPSLPFVPALFVFGDSSVDSGTNNFLGTLARADRLPYGRDFDTHQPT 109

Query: 103 GRFCNGRIPVDFLALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHI 162
           GRFCNGRIPVD+    LGLPFVPSYLG  G VEDM QGVNYASA AG+I +SGSELGQ +
Sbjct: 110 GRFCNGRIPVDY----LGLPFVPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSELGQRV 169

Query: 163 SFTQQIQQFMDTFQQFVLSMGEKNAADHISNSVFYISIGINDYIHYYLFNISSVQNLYSP 222
           SF  Q++QF+DTFQQ +LS+GEK +   +SNSVFYISIG+NDYIH+Y+ NIS+VQNLY+P
Sbjct: 170 SFAMQVEQFVDTFQQMILSIGEKASERLVSNSVFYISIGVNDYIHFYIRNISNVQNLYTP 229

Query: 223 WNFNQFLAATIRQEIKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVM 282
           WNFNQFLA+ +RQE+K LYN+  RR+V+MGL PIGCAP+Y+W+Y S+NGEC EE+N M+M
Sbjct: 230 WNFNQFLASNMRQELKTLYNVKVRRMVVMGLPPIGCAPYYMWKYRSQNGECAEEVNSMIM 289

Query: 283 EFNFAMRYMVEELGMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWI 342
           E NF MRY V++L  ELP +SII+CD+ + +MDIL+NH+ YGFN TTDACCG GRY GW+
Sbjct: 290 ESNFVMRYTVDKLNRELPGASIIYCDVFQSAMDILRNHQHYGFNETTDACCGLGRYKGWL 349

Query: 343 MCISPVMACKNASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDIL 396
            CISP MAC +AS H+WWDQFHPTDAVNAILA+NVWNG H  MCYP NL+ +L
Sbjct: 350 PCISPEMACSDASGHLWWDQFHPTDAVNAILADNVWNGRHVDMCYPTNLETML 398

BLAST of MS012842 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 7.6e-68
Identity = 137/354 (38.70%), Postives = 197/354 (55.65%), Query Frame = 0

Query: 49  PISPVAPPP-------SNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSP 108
           PIS  APPP        + +VPALFV GDS +D G NN + +FA+A++ PYG DF+   P
Sbjct: 35  PIS--APPPPLVDLNTGDGIVPALFVFGDSLIDNGNNNNIPSFAKANYFPYGIDFN-GGP 94

Query: 109 TGRFCNGRIPVDFLALRLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQH 168
           TGRFCNG   VD +A  LGLP +P+Y    G  + +++GVNYASA+AG++  +G      
Sbjct: 95  TGRFCNGLTMVDGIAQLLGLPLIPAYSEATG--DQVLRGVNYASAAAGILPDTGGNFVGR 154

Query: 169 ISFTQQIQQFMDTFQQFVLSMGEKNA-ADHISNSVFYISIGINDYIHYYLFNISSVQNLY 228
           I F QQI  F  T  Q     G   A AD ++ S+F+I +G NDY++ YL      +N Y
Sbjct: 155 IPFDQQIHNFETTLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQY 214

Query: 229 SPWNFNQFLAATIRQEIKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDM 288
           +   F   L      ++  LYN+  R+ V+ GL  +GC P  L Q    +G+C EE+N +
Sbjct: 215 NSQQFGDLLVQHYTDQLTRLYNLGGRKFVVAGLGRMGCIPSILAQ--GNDGKCSEEVNQL 274

Query: 289 VMEFNFAMRYMVEELGMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNG 348
           V+ FN  ++ M+  L   LPD+  I+ D+     DI+ N   YG       CCG G+  G
Sbjct: 275 VLPFNTNVKTMISNLNQNLPDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRG 334

Query: 349 WIMCISPVMACKNASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
            I C+     C N   +++WD FHPT+ VN I+A+  + G   T+ YP N++ +
Sbjct: 335 QITCLPFETPCPNRDQYVFWDAFHPTEKVNLIMAKKAFAG-DRTVAYPINIQQL 380

BLAST of MS012842 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 9.3e-66
Identity = 125/341 (36.66%), Postives = 193/341 (56.60%), Query Frame = 0

Query: 58  SNSLVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLAL 117
           + ++ PA+FV GDS VD G NN L + AR+++LPYG DF  + PTGRF NG+  VDF+  
Sbjct: 43  TTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGE 102

Query: 118 RLGLPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQ 177
            LGLP +P+++       D++ GVNYASA+ G++  +G  LG+  S  +Q++ F  T  +
Sbjct: 103 LLGLPEIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLME 162

Query: 178 FVLSMGEKNAADHISNSVFYISIGINDYIHYYLF-NISSVQNLYSPWNFNQFLAATIRQE 237
              SM +++  ++++ S+  +S+G NDYI+ YL   +    ++Y P +F   L +     
Sbjct: 163 ISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTH 222

Query: 238 IKNLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELG 297
           +  LY    R+ VI G+ P+GC P  L    +  GEC+E +N+M   FN  +  +V+ L 
Sbjct: 223 LLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLN 282

Query: 298 MELPDSS---IIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKN 357
            +   +S    ++ +    ++DIL N   YGF VT   CCG GR  G I C+   + C  
Sbjct: 283 SDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCAF 342

Query: 358 ASNHIWWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
              H++WD FHPT A N I+A   +NG  +  CYP NL  +
Sbjct: 343 RDRHVFWDAFHPTQAFNLIIALRAFNGSKSD-CYPINLSQL 382

BLAST of MS012842 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 242.7 bits (618), Expect = 5.1e-64
Identity = 129/324 (39.81%), Postives = 188/324 (58.02%), Query Frame = 0

Query: 61  LVPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLG 120
           L PA FV GDS VD+G NN++ T ARA++ PYG DF    PTGRFCNGR  VD+ A  LG
Sbjct: 27  LAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFG--FPTGRFCNGRTVVDYGATYLG 86

Query: 121 LPFVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 180
           LP VP YL      ++ ++GVNYASA+AG++  +G   G   +F  QI QF  T  +  L
Sbjct: 87  LPLVPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITI-ELRL 146

Query: 181 SMGEKNAAD---HISNSVFYISIGINDYIHYYLF-NISSVQNLYSPWNFNQFLAATIRQE 240
               +N AD   +++ S+  I+IG NDYI+ YL     S    YS  ++   L  T+  +
Sbjct: 147 RRFFQNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQ 206

Query: 241 IKNLYNMNARRIVIMGLAPIGCAPFYLWQY-GSENGECIEEINDMVMEFNFAMRYMVEEL 300
           I  LYN+ AR++V+ G  P+GC P  L    G+    C+ +IN+MV  FN  ++ +   L
Sbjct: 207 ISRLYNLGARKMVLAGSGPLGCIPSQLSMVTGNNTSGCVTKINNMVSMFNSRLKDLANTL 266

Query: 301 GMELPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNAS 360
              LP S  ++ ++ +   D++ N  RYG  V+ +ACCG GRY G + C+     C + +
Sbjct: 267 NTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDRN 326

Query: 361 NHIWWDQFHPTDAVNAILAENVWN 380
            +++WD FHPT+  N I+A N ++
Sbjct: 327 QYVFWDAFHPTETANKIIAHNTFS 347

BLAST of MS012842 vs. TAIR 10
Match: AT1G29660.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.7e-62
Identity = 123/336 (36.61%), Postives = 189/336 (56.25%), Query Frame = 0

Query: 62  VPALFVIGDSTVDAGTNNFLGTFARADHLPYGRDFDTHSPTGRFCNGRIPVDFLALRLGL 121
           VP  F+ GDS VD G NN L + ARAD+ PYG DF    PTGRF NGR  VD L   LG 
Sbjct: 30  VPCYFIFGDSLVDNGNNNRLRSIARADYFPYGIDFG--GPTGRFSNGRTTVDVLTELLGF 89

Query: 122 P-FVPSYLGHAGAVEDMIQGVNYASASAGVIFTSGSELGQHISFTQQIQQFMDTFQQFVL 181
             ++P+Y   +G  ++++QGVNYASA+AG+   +G++LGQ I+F+ Q++ + +T  Q V 
Sbjct: 90  DNYIPAYSTVSG--QEILQGVNYASAAAGIREETGAQLGQRITFSGQVENYKNTVAQVVE 149

Query: 182 SMG-EKNAADHISNSVFYISIGINDYI-HYYLFNISSVQNLYSPWNFNQFLAATIRQEIK 241
            +G E  AAD++   ++ + +G NDY+ +Y++    S    Y+P  +   L +  R ++ 
Sbjct: 150 ILGDEYTAADYLKRCIYSVGMGSNDYLNNYFMPQFYSTSRQYTPEQYADDLISRYRDQLN 209

Query: 242 NLYNMNARRIVIMGLAPIGCAPFYLWQYGSENGECIEEINDMVMEFNFAMRYMVEELGME 301
            LYN  AR+  ++G+  IGC+P  L Q   +   C+E IN     FN  +  MV++L   
Sbjct: 210 ALYNYGARKFALVGIGAIGCSPNALAQGSQDGTTCVERINSANRIFNNRLISMVQQLNNA 269

Query: 302 LPDSSIIFCDLLEGSMDILKNHERYGFNVTTDACCGFGRYNGWIMCISPVMACKNASNHI 361
             D+S  + +      DI+ N   YGF  T  ACCG GR  G + C+     C N   ++
Sbjct: 270 HSDASFTYINAYGAFQDIIANPSAYGFTNTNTACCGIGRNGGQLTCLPGEPPCLNRDEYV 329

Query: 362 WWDQFHPTDAVNAILAENVWNGLHTTMCYPKNLKDI 395
           +WD FHP+ A N  +A+  +N   ++  YP ++  +
Sbjct: 330 FWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDISQL 361