MS012829 (gene) Bitter gourd (TR) v1

BLAST of MS012829 vs. NCBI nr
Match: XP_022136555.1 (long chain acyl-CoA synthetase 4-like [Momordica charantia])

HSP 1 Score: 1320.8 bits (3417), Expect = 0.0e+00
Identity = 647/647 (100.00%), Postives = 647/647 (100.00%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK
Sbjct: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ
Sbjct: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 647

BLAST of MS012829 vs. NCBI nr
Match: XP_022931292.1 (long chain acyl-CoA synthetase 4-like [Cucurbita moschata])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 599/660 (90.76%), Postives = 632/660 (95.76%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEE+K A DGRPSIGPVYRS FAKDGFPPPIEGL+SCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPPPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVN KAGKYVWLTYKEVYD+VIK+GNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEIS+ FVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKV PNLREEFEKYG+ IFSWDE LLLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL+SVNEE+  K+VYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLL+EDIGELKPSIFCAVPRVLDRIYAGL+Q++++G FF+K LFNLAYS K+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H +ASPI DK+VFSKVKQGLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGDASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN LALERWA+ENGIEGDFDSLCEDKRAKDYLLGE+N
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGEIN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIYQFS 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+    + D +Y+ S
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYK---NVIDSMYKNS 657

BLAST of MS012829 vs. NCBI nr
Match: XP_022985381.1 (long chain acyl-CoA synthetase 4-like [Cucurbita maxima])

HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 599/660 (90.76%), Postives = 630/660 (95.45%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEE+K A DGRPSIGPVYRS FAKDGFP PIEGL+SCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVN KAGKYVWLTYKEVYD+VIK+GNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEIS+ FVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYG+ IFSWDE LLLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL+SVNEE+  K+VYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLL+EDIGELKPSIFCAVPRVLDRIYAGL+Q++++G FF+K LFNLAYS K+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H EASPI DK+VFSKVKQGLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN LALERWA ENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWAKENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIYQFS 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+    + D +Y+ S
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYK---NVIDSMYKNS 657

BLAST of MS012829 vs. NCBI nr
Match: KAG7015143.1 (Long chain acyl-CoA synthetase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 597/660 (90.45%), Postives = 631/660 (95.61%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEE+K A DGRPSIGPVYRS FAKDGFP PIEGL+SCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVN KAGKYVWLTYKEVYD+VIK+GNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEIS+ FVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYG+ IFSW+E LLLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVEPNLKEEFEKYGLEIFSWEEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL+SVNEE+  K+VYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLL+EDIGELKPSIFCAVPRVLDRIYAGL+Q++++G FF+K LFNLAYS K+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H EASPI DK+VFSKVKQGLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN LALERWA+ENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIYQFS 660
           +I+KEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+    + D +Y+ S
Sbjct: 601 KISKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYK---NVIDSMYKNS 657

BLAST of MS012829 vs. NCBI nr
Match: XP_023552641.1 (long chain acyl-CoA synthetase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 596/660 (90.30%), Postives = 631/660 (95.61%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEE+K A DGRPSIGPVYRS FAKDGFP PIEGL+SCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVN KAGKYVWLTYKEVYD+VIK+GNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGL+CVPLYDTLGAGAVEFIICHAEIS+ FVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLFCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYG+ IFSWDE LLLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL+SVNEE+  K+VYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLL+EDIGELKPSIFCAVPRVLDRIYAGL+Q++++G FF+K LFNLAYS K+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H EASPI DK+VFSKVKQGLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPN+LSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNKLSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN LALERWA+ENGIEGDFD+LCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDALCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIYQFS 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+    + D +Y+ S
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYK---NVIDSMYKNS 657

BLAST of MS012829 vs. ExPASy Swiss-Prot
Match: Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 1.5e-302
Identity = 485/645 (75.19%), Postives = 571/645 (88.53%), Query Frame = 0

Query: 3   QMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNRM 62
           Q KY+ QVEE KE  DGRPS+GPVYRS FAKDGFP PIEG++SCWD+FR+SVE+ P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 63  LGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISMEA 122
           LGRREIV+ K GKYVW TY+EVYDIV+K+GNSLRS G ++ AKCGIYGAN  EWIISMEA
Sbjct: 64  LGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEA 123

Query: 123 CNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSFG 182
           CNAHGLYCVPLYDTLGA AVEFII H+E+SI FVEEKK+ E+ KT PN+T+ +KT+VSFG
Sbjct: 124 CNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFG 183

Query: 183 KVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVMI 242
            V+   +EE E +G+ I++WDE L LGE + +DLP KK+SDI TIMYTSGTTGDPKGVMI
Sbjct: 184 GVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMI 243

Query: 243 SNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRGD 302
           SN+SI++LIAGV RLL+S NE LT K+VYLSYLPLAHIFDRVIEE FI HGA+IGFWRGD
Sbjct: 244 SNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGD 303

Query: 303 VKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKGH 362
           VKLLIED+ ELKP+IFCAVPRVLDR+Y+GL +K+  G F KK +F+ A+S KFG M+KG 
Sbjct: 304 VKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQ 363

Query: 363 NHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTET 422
           +H EASP+ DK+VFSKVKQGLGGNVR+ILSGAAPL++HVE+FLRVVAC HVLQGYGLTE+
Sbjct: 364 SHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTES 423

Query: 423 CAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYY 482
           CAGTFVSLP+EL MLGTVGPPVPNVD+ LESVPEM YDAL++T RGEIC+RG  LFSGYY
Sbjct: 424 CAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYY 483

Query: 483 KRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQVS 542
           KR+DLT EV+IDGW HTGDVGEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+ENIYG+V 
Sbjct: 484 KREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQ 543

Query: 543 SIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELNRI 602
           ++D +WVYGNSFESFL+A+ANPN   LERWA+ENG+ GD+D+LC++++AK+++LGEL ++
Sbjct: 544 AVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKM 603

Query: 603 AKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           AKEKK+KGFE IKAIHLDP+PFDMERDL+TPT+KKKRPQLLKYYQ
Sbjct: 604 AKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQ 648

BLAST of MS012829 vs. ExPASy Swiss-Prot
Match: Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 3.0e-295
Identity = 476/646 (73.68%), Postives = 553/646 (85.60%), Query Frame = 0

Query: 2   SQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNR 61
           SQ +++ +VE AKEA DG PS+GPVYRS+FA++GFP PI+G+ SCWDIFR +VE+ P NR
Sbjct: 3   SQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNR 62

Query: 62  MLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISME 121
           MLGRREI N KAGKYVW TYKEVYDIVIK+GNSLRS G +EG KCGIYG NC EWIISME
Sbjct: 63  MLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISME 122

Query: 122 ACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSF 181
           ACNAHGLYCVPLYDTLGAGAVEFII HAE+SIAFVEEKK+PE+ KT PN+TK +KT+VSF
Sbjct: 123 ACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSF 182

Query: 182 GKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVM 241
           G V P  +EE EK G+ I SWDE L LGE + ++LP KK SDI TIMYTSGTTGDPKGVM
Sbjct: 183 GGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVM 242

Query: 242 ISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRG 301
           ISN+SI+++  GV   L +VN  L+EK+VY+SYLPLAH+FDR IEE  I  G SIGFWRG
Sbjct: 243 ISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRG 302

Query: 302 DVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKG 361
           DVKLLIED+GELKPSIFCAVPRVLDR+Y GL QK+  G FFKK +F++A+S KFGNM+KG
Sbjct: 303 DVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKG 362

Query: 362 HNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTE 421
            +H  ASP  DK+VF+KVKQGLGGNVR+ILSGAAPL++H+E+FLRVVAC +VLQGYGLTE
Sbjct: 363 QSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTE 422

Query: 422 TCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGY 481
           +CAGTF + P+EL MLGTVGPPVPNVD+ LESVPEM YDAL +TPRGEIC+RG  LFSGY
Sbjct: 423 SCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGY 482

Query: 482 YKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQV 541
           YKR+DLT EV IDGW HTGDVGEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLEN+Y QV
Sbjct: 483 YKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQV 542

Query: 542 SSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELNR 601
             I+ IWVYGNSFESFLVA+ANP    LERWA ENG+ GDF+S+C++ +AK ++LGEL +
Sbjct: 543 EVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVK 602

Query: 602 IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
            AKE KLKGFE IK +HL+P+ FDMERDL+TPTYKKKRPQLLKYYQ
Sbjct: 603 TAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQ 648

BLAST of MS012829 vs. ExPASy Swiss-Prot
Match: Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 1.3e-287
Identity = 463/651 (71.12%), Postives = 557/651 (85.56%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKD-GRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPG 60
           M+  +Y+++VE+ K+  D G PS+GPVYRS +AKDGFP P + L S WDIFRLSVE++P 
Sbjct: 1   MATGRYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPN 60

Query: 61  NRMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIIS 120
           N MLGRREIV+ KAGKYVW TYKEV+++VIK+GNS+R+ G  +G KCGIYGAN  EWIIS
Sbjct: 61  NPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIIS 120

Query: 121 MEACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIV 180
           MEACNAHGLYCVPLYDTLGAGA+EFIICHAE+S+AF EE K+ E+LKT P +TK LK IV
Sbjct: 121 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIV 180

Query: 181 SFGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKG 240
           SFG+VT N R E E++ + I+SWD+ L LGE +H++LPEK+RSD+ TIMYTSGTTGDPKG
Sbjct: 181 SFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKG 240

Query: 241 VMISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFW 300
           V+++N+SII L+ GV++LL++++EELT K+VYLSYLPLAHIFDRVIEEL I   ASIGFW
Sbjct: 241 VLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFW 300

Query: 301 RGDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQ 360
           RGDVK+LIEDI  LKP++FCAVPRVL+RIY GL QK+  G F KK LFN A+  K  NM+
Sbjct: 301 RGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNME 360

Query: 361 KGHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGL 420
           KG  H +ASPI+DKIVF KVK+GLGGNVRLILSGAAPL+ H+E+FLRVVACAHVLQGYGL
Sbjct: 361 KGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGL 420

Query: 421 TETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFS 480
           TE+C GTFVS+PNELSMLGTVGPPVPNVD+ LESVPEMGYDAL++ PRGEIC+RG  LFS
Sbjct: 421 TESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFS 480

Query: 481 GYYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 540
           GYYKR+DLT EV IDGW HTGDVGEWQPDG++KIIDRKKNIFKLSQGEYVAVENLENIY 
Sbjct: 481 GYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYS 540

Query: 541 QVSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGEL 600
            V++I+ IWVYGNS+ES+LVAV  P+ + +E WA E+ + GDF+S+C +++ K+++LGE 
Sbjct: 541 HVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEF 600

Query: 601 NRIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRI 651
           NR+AK+KKLKGFE IK +HLD +PFDMERDLITP+YK KRPQLLKYYQ  I
Sbjct: 601 NRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEI 651

BLAST of MS012829 vs. ExPASy Swiss-Prot
Match: M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 1.2e-227
Identity = 369/644 (57.30%), Postives = 481/644 (74.69%), Query Frame = 0

Query: 4   MKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNRML 63
           M Y ++VEEA+ A +  PS GPVYRS +A+DG     EGL S W+    SV+R+P   ML
Sbjct: 1   MSYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEFLSGSVKRSPKTPML 60

Query: 64  GRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISMEAC 123
           GRR+I + +AG YVWLTY+EV+D  I++ +++RSRG   G +CGIYG NC +WI++M+AC
Sbjct: 61  GRRQIKDSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQWIVAMQAC 120

Query: 124 NAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSFGK 183
            +H +  VPLYDTLG  AVEFII H E+SIAFV+E K+  +L   PN +  LKTIVSFG 
Sbjct: 121 YSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLPNCSSLLKTIVSFGN 180

Query: 184 VTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVMIS 243
           +T   ++E E  GV  FSW+E   LG N   +LPEK R+DI T+MYTSG TG+PKGV+++
Sbjct: 181 ITSVQKKETEALGVSCFSWEEFSQLG-NLSGELPEKHRTDICTLMYTSGATGEPKGVILT 240

Query: 244 NKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDV 303
           N++I++ I     +L+  ++  +E++VY SYLPLAH++D+++E   I  G++IG+WRGD+
Sbjct: 241 NEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVENYCIYKGSAIGYWRGDI 300

Query: 304 KLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKGHN 363
           + L++D+ ELKP+IFC VPRV DRIYAG+  KV SG   KK+LF  AY+ K  NM+KG  
Sbjct: 301 RFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLFQYAYNYKMANMEKGLP 360

Query: 364 HAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTETC 423
           H +A+P+ DK+ F K+KQG GG +RL+ SGAAPL  HVE +LRV +CA + QGYGLTE+C
Sbjct: 361 HGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRVTSCAALSQGYGLTESC 420

Query: 424 AGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYK 483
            G   S+ N   M+GTVG P+  ++  LESVPEMGYDALS  PRGEIC+RG  LFSGY+K
Sbjct: 421 GGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPRGEICLRGTTLFSGYHK 480

Query: 484 RDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQVSS 543
           R+DLT +V++DGWFHTGD+GEWQP+G++KIIDRKKNIFKLSQGEYV VEN+E IY Q   
Sbjct: 481 REDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGEYVPVENIEGIYLQCPL 540

Query: 544 IDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELNRIA 603
           I  IWVYGNSFESFLVAV  P  LALE WA+   +  DF SLCE+ +A  Y+L ELN  A
Sbjct: 541 IASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCENPKASKYILDELNSTA 600

Query: 604 KEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           K+ +L+GFE +KA++L+P PFDMERDLITPT+K KRPQLLKYY+
Sbjct: 601 KKHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYK 643

BLAST of MS012829 vs. ExPASy Swiss-Prot
Match: Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 3.1e-212
Identity = 348/653 (53.29%), Postives = 471/653 (72.13%), Query Frame = 0

Query: 7   LIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNRMLGRR 66
           ++ VEE + A    PS GPVYR  +AKDG       ++S W  F  +V++ P  +MLG+R
Sbjct: 8   VLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQR 67

Query: 67  EIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISMEACNAH 126
              + K G Y W+TYKE +D  I++G+++RSRG + G  CGIYGANC EWII+MEAC + 
Sbjct: 68  VTTDSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 127

Query: 127 GLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSFGKVTP 186
           G+  VPLYD+LG  AVEFII HAE+S+ FV+EK +  +L      +  LKTIVSFG+V+ 
Sbjct: 128 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 187

Query: 187 NLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVMISNKS 246
             +EE +   V +FSW+E  L+G     +LP K+++DI TIMYTSGTTG+PKGV+++N +
Sbjct: 188 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 247

Query: 247 IISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLL 306
           I   +  + ++L+  +      +V+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ L
Sbjct: 248 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 307

Query: 307 IEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKGHNHAE 366
           ++D+  LKP++FC VPRV D++YAG+ QK+ +    +K LF+ AY+ K GNM+KG +  E
Sbjct: 308 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 367

Query: 367 ASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTETCAGT 426
           ASP  D+++F K+K+ LGG   ++LSGAAPL  HVE FLR++  +++ QGYGLTE+C G+
Sbjct: 368 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 427

Query: 427 FVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST-TPRGEICVRGDPLFSGYYKRD 486
           F +L    SM+GTVG P+P V+  L SVPEMGYDA S   PRGEIC+RG+ +FSGY+KR 
Sbjct: 428 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 487

Query: 487 DLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQVSSID 546
           DLT++V+IDGWFHTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLEN Y +   I 
Sbjct: 488 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 547

Query: 547 MIWVYGNSFESFLVAVANPNMLALERWASEN-GIEGDFDSLCEDKRAKDYLLGELNRIAK 606
            IWVYGNSFESFLV V  P+  A+E WA  N     DF+SLC++ +A+ Y L ELN  AK
Sbjct: 548 QIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAK 607

Query: 607 EKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIY 658
           + +LKGFE +KAIHL+P PFD+ERDLITPT+K KRPQLL++Y+    I D +Y
Sbjct: 608 QYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYK---GIVDQLY 657

BLAST of MS012829 vs. ExPASy TrEMBL
Match: A0A6J1C489 (long chain acyl-CoA synthetase 4-like OS=Momordica charantia OX=3673 GN=LOC111008230 PE=4 SV=1)

HSP 1 Score: 1320.8 bits (3417), Expect = 0.0e+00
Identity = 647/647 (100.00%), Postives = 647/647 (100.00%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK
Sbjct: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ
Sbjct: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 647

BLAST of MS012829 vs. ExPASy TrEMBL
Match: A0A6J1ET87 (long chain acyl-CoA synthetase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111437522 PE=4 SV=1)

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 599/660 (90.76%), Postives = 632/660 (95.76%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEE+K A DGRPSIGPVYRS FAKDGFPPPIEGL+SCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPPPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVN KAGKYVWLTYKEVYD+VIK+GNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEIS+ FVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKV PNLREEFEKYG+ IFSWDE LLLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLREEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL+SVNEE+  K+VYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLL+EDIGELKPSIFCAVPRVLDRIYAGL+Q++++G FF+K LFNLAYS K+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H +ASPI DK+VFSKVKQGLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGDASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN LALERWA+ENGIEGDFDSLCEDKRAKDYLLGE+N
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGEIN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIYQFS 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+    + D +Y+ S
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYK---NVIDSMYKNS 657

BLAST of MS012829 vs. ExPASy TrEMBL
Match: A0A6J1J803 (long chain acyl-CoA synthetase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111483406 PE=4 SV=1)

HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 599/660 (90.76%), Postives = 630/660 (95.45%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLIQVEE+K A DGRPSIGPVYRS FAKDGFP PIEGL+SCWDIFRLSVERNPGN
Sbjct: 1   MSQMKYLIQVEESKAAIDGRPSIGPVYRSIFAKDGFPLPIEGLDSCWDIFRLSVERNPGN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRREIVN KAGKYVWLTYKEVYD+VIK+GNSLRSRGFEEGAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRREIVNGKAGKYVWLTYKEVYDMVIKIGNSLRSRGFEEGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICHAEIS+ FVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISVVFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKV PNL+EEFEKYG+ IFSWDE LLLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVAPNLKEEFEKYGLEIFSWDEFLLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL+SVNEE+  K+VYLSYLPLAHIFDRV+EELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLESVNEEMNAKDVYLSYLPLAHIFDRVLEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKLL+EDIGELKPSIFCAVPRVLDRIYAGL+Q++++G FF+K LFNLAYS K+GNMQK
Sbjct: 301 GDVKLLVEDIGELKPSIFCAVPRVLDRIYAGLTQRLDAGGFFRKTLFNLAYSHKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H EASPI DK+VFSKVKQGLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHGEASPICDKLVFSKVKQGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRG+PLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGEPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VS+IDMIWVYGNSFESFLVAVANPN LALERWA ENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSAIDMIWVYGNSFESFLVAVANPNKLALERWAKENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIYQFS 660
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYY+    + D +Y+ S
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYK---NVIDSMYKNS 657

BLAST of MS012829 vs. ExPASy TrEMBL
Match: A0A5A7TP41 (Long chain acyl-CoA synthetase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001980 PE=4 SV=1)

HSP 1 Score: 1225.3 bits (3169), Expect = 0.0e+00
Identity = 590/647 (91.19%), Postives = 622/647 (96.14%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLI+VE+ K A DGRPSIGPVYRS FAKDGFPPPI+GL+SCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIDGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRRE VN KAGKYVWLTYKEVYD V+KVGNSLR+RGFE+GAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+AFVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEK+GV IFSWDE  LLGENQHFDLP KK+SDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKKSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL++VNEEL+ K+VYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKL+IEDIGELKPSIFCAVPRVLDRIYAGL+Q+++ G   K+ LFN+AYSRK+GNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H+EASPI D+ VF+KVK+GLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN LALERWA+ENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 647

BLAST of MS012829 vs. ExPASy TrEMBL
Match: A0A1S3BTT7 (long chain acyl-CoA synthetase 4-like OS=Cucumis melo OX=3656 GN=LOC103493094 PE=4 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 590/647 (91.19%), Postives = 621/647 (95.98%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGN 60
           MSQMKYLI+VE+ K A  GRPSIGPVYRS FAKDGFPPPI+GL+SCWDIFRLSVERNP N
Sbjct: 1   MSQMKYLIEVEKPKPAIGGRPSIGPVYRSIFAKDGFPPPIQGLDSCWDIFRLSVERNPDN 60

Query: 61  RMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISM 120
           RMLGRRE VN KAGKYVWLTYKEVYD V+KVGNSLR+RGFE+GAKCGIYGANCSEWIISM
Sbjct: 61  RMLGRRETVNGKAGKYVWLTYKEVYDTVVKVGNSLRTRGFEQGAKCGIYGANCSEWIISM 120

Query: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVS 180
           EACNAHGLYCVPLYDTLGAGAVEFIICH+EIS+AFVEEKKLPEVLKTFP+TTK LKTIVS
Sbjct: 121 EACNAHGLYCVPLYDTLGAGAVEFIICHSEISVAFVEEKKLPEVLKTFPSTTKYLKTIVS 180

Query: 181 FGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGV 240
           FGKVTPNLREEFEK+GV IFSWDE  LLGENQHFDLP KKRSDISTIMYTSGTTGDPKGV
Sbjct: 181 FGKVTPNLREEFEKHGVEIFSWDEFSLLGENQHFDLPVKKRSDISTIMYTSGTTGDPKGV 240

Query: 241 MISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300
           MISNKSIISLIAGVQRLL++VNEEL+ K+VYLSYLPLAHIFDRVIEELFILHGASIGFWR
Sbjct: 241 MISNKSIISLIAGVQRLLENVNEELSPKDVYLSYLPLAHIFDRVIEELFILHGASIGFWR 300

Query: 301 GDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQK 360
           GDVKL+IEDIGELKPSIFCAVPRVLDRIYAGL+Q+++ G   K+ LFN+AYSRK+GNMQK
Sbjct: 301 GDVKLIIEDIGELKPSIFCAVPRVLDRIYAGLTQRLDQGGLLKRTLFNIAYSRKYGNMQK 360

Query: 361 GHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLT 420
           GH H+EASPI D+ VF+KVK+GLGGNVRLILSGAAPL+THVEAFLRVVACAHVLQGYGLT
Sbjct: 361 GHKHSEASPICDRFVFNKVKEGLGGNVRLILSGAAPLATHVEAFLRVVACAHVLQGYGLT 420

Query: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480
           ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG
Sbjct: 421 ETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSG 480

Query: 481 YYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQ 540
           YYKRDDLTNEVMIDGWFHTGD+GEWQ DGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 
Sbjct: 481 YYKRDDLTNEVMIDGWFHTGDIGEWQSDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGL 540

Query: 541 VSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELN 600
           VSSIDMIWVYGNSFESFLVAVANPN LALERWA+ENGIEGDFDSLCEDKRAKDYLLGELN
Sbjct: 541 VSSIDMIWVYGNSFESFLVAVANPNKLALERWANENGIEGDFDSLCEDKRAKDYLLGELN 600

Query: 601 RIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           +IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ
Sbjct: 601 KIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 647

BLAST of MS012829 vs. TAIR 10
Match: AT4G23850.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1039.6 bits (2687), Expect = 1.0e-303
Identity = 485/645 (75.19%), Postives = 571/645 (88.53%), Query Frame = 0

Query: 3   QMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNRM 62
           Q KY+ QVEE KE  DGRPS+GPVYRS FAKDGFP PIEG++SCWD+FR+SVE+ P N M
Sbjct: 4   QKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRMSVEKYPNNPM 63

Query: 63  LGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISMEA 122
           LGRREIV+ K GKYVW TY+EVYDIV+K+GNSLRS G ++ AKCGIYGAN  EWIISMEA
Sbjct: 64  LGRREIVDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEA 123

Query: 123 CNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSFG 182
           CNAHGLYCVPLYDTLGA AVEFII H+E+SI FVEEKK+ E+ KT PN+T+ +KT+VSFG
Sbjct: 124 CNAHGLYCVPLYDTLGADAVEFIISHSEVSIVFVEEKKISELFKTCPNSTEYMKTVVSFG 183

Query: 183 KVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVMI 242
            V+   +EE E +G+ I++WDE L LGE + +DLP KK+SDI TIMYTSGTTGDPKGVMI
Sbjct: 184 GVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTSGTTGDPKGVMI 243

Query: 243 SNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRGD 302
           SN+SI++LIAGV RLL+S NE LT K+VYLSYLPLAHIFDRVIEE FI HGA+IGFWRGD
Sbjct: 244 SNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFDRVIEECFIQHGAAIGFWRGD 303

Query: 303 VKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKGH 362
           VKLLIED+ ELKP+IFCAVPRVLDR+Y+GL +K+  G F KK +F+ A+S KFG M+KG 
Sbjct: 304 VKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFLKKFIFDSAFSYKFGYMKKGQ 363

Query: 363 NHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTET 422
           +H EASP+ DK+VFSKVKQGLGGNVR+ILSGAAPL++HVE+FLRVVAC HVLQGYGLTE+
Sbjct: 364 SHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVESFLRVVACCHVLQGYGLTES 423

Query: 423 CAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYY 482
           CAGTFVSLP+EL MLGTVGPPVPNVD+ LESVPEM YDAL++T RGEIC+RG  LFSGYY
Sbjct: 424 CAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDALASTARGEICIRGKTLFSGYY 483

Query: 483 KRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQVS 542
           KR+DLT EV+IDGW HTGDVGEWQPDGS+KIIDRKKNIFKLSQGEYVAVEN+ENIYG+V 
Sbjct: 484 KREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQ 543

Query: 543 SIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELNRI 602
           ++D +WVYGNSFESFL+A+ANPN   LERWA+ENG+ GD+D+LC++++AK+++LGEL ++
Sbjct: 544 AVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDYDALCQNEKAKEFILGELVKM 603

Query: 603 AKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
           AKEKK+KGFE IKAIHLDP+PFDMERDL+TPT+KKKRPQLLKYYQ
Sbjct: 604 AKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQLLKYYQ 648

BLAST of MS012829 vs. TAIR 10
Match: AT4G11030.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 1015.4 bits (2624), Expect = 2.1e-296
Identity = 476/646 (73.68%), Postives = 553/646 (85.60%), Query Frame = 0

Query: 2   SQMKYLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNR 61
           SQ +++ +VE AKEA DG PS+GPVYRS+FA++GFP PI+G+ SCWDIFR +VE+ P NR
Sbjct: 3   SQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFRTAVEKYPNNR 62

Query: 62  MLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISME 121
           MLGRREI N KAGKYVW TYKEVYDIVIK+GNSLRS G +EG KCGIYG NC EWIISME
Sbjct: 63  MLGRREISNGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISME 122

Query: 122 ACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSF 181
           ACNAHGLYCVPLYDTLGAGAVEFII HAE+SIAFVEEKK+PE+ KT PN+TK +KT+VSF
Sbjct: 123 ACNAHGLYCVPLYDTLGAGAVEFIISHAEVSIAFVEEKKIPELFKTCPNSTKYMKTVVSF 182

Query: 182 GKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVM 241
           G V P  +EE EK G+ I SWDE L LGE + ++LP KK SDI TIMYTSGTTGDPKGVM
Sbjct: 183 GGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGTTGDPKGVM 242

Query: 242 ISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRG 301
           ISN+SI+++  GV   L +VN  L+EK+VY+SYLPLAH+FDR IEE  I  G SIGFWRG
Sbjct: 243 ISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFDRAIEECIIQVGGSIGFWRG 302

Query: 302 DVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKG 361
           DVKLLIED+GELKPSIFCAVPRVLDR+Y GL QK+  G FFKK +F++A+S KFGNM+KG
Sbjct: 303 DVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFFKKKVFDVAFSYKFGNMKKG 362

Query: 362 HNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTE 421
            +H  ASP  DK+VF+KVKQGLGGNVR+ILSGAAPL++H+E+FLRVVAC +VLQGYGLTE
Sbjct: 363 QSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIESFLRVVACCNVLQGYGLTE 422

Query: 422 TCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGY 481
           +CAGTF + P+EL MLGTVGPPVPNVD+ LESVPEM YDAL +TPRGEIC+RG  LFSGY
Sbjct: 423 SCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDALGSTPRGEICIRGKTLFSGY 482

Query: 482 YKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQV 541
           YKR+DLT EV IDGW HTGDVGEWQP+GS+KIIDRKKNIFKL+QGEYVAVENLEN+Y QV
Sbjct: 483 YKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQV 542

Query: 542 SSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELNR 601
             I+ IWVYGNSFESFLVA+ANP    LERWA ENG+ GDF+S+C++ +AK ++LGEL +
Sbjct: 543 EVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDFNSICQNAKAKAFILGELVK 602

Query: 602 IAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQ 648
            AKE KLKGFE IK +HL+P+ FDMERDL+TPTYKKKRPQLLKYYQ
Sbjct: 603 TAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQLLKYYQ 648

BLAST of MS012829 vs. TAIR 10
Match: AT1G64400.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 989.9 bits (2558), Expect = 9.5e-289
Identity = 463/651 (71.12%), Postives = 557/651 (85.56%), Query Frame = 0

Query: 1   MSQMKYLIQVEEAKEAKD-GRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPG 60
           M+  +Y+++VE+ K+  D G PS+GPVYRS +AKDGFP P + L S WDIFRLSVE++P 
Sbjct: 1   MATGRYIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRLSVEKSPN 60

Query: 61  NRMLGRREIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIIS 120
           N MLGRREIV+ KAGKYVW TYKEV+++VIK+GNS+R+ G  +G KCGIYGAN  EWIIS
Sbjct: 61  NPMLGRREIVDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIIS 120

Query: 121 MEACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIV 180
           MEACNAHGLYCVPLYDTLGAGA+EFIICHAE+S+AF EE K+ E+LKT P +TK LK IV
Sbjct: 121 MEACNAHGLYCVPLYDTLGAGAIEFIICHAEVSLAFAEENKISELLKTAPKSTKYLKYIV 180

Query: 181 SFGKVTPNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKG 240
           SFG+VT N R E E++ + I+SWD+ L LGE +H++LPEK+RSD+ TIMYTSGTTGDPKG
Sbjct: 181 SFGEVTNNQRVEAERHRLTIYSWDQFLKLGEGKHYELPEKRRSDVCTIMYTSGTTGDPKG 240

Query: 241 VMISNKSIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFW 300
           V+++N+SII L+ GV++LL++++EELT K+VYLSYLPLAHIFDRVIEEL I   ASIGFW
Sbjct: 241 VLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEELCIYEAASIGFW 300

Query: 301 RGDVKLLIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQ 360
           RGDVK+LIEDI  LKP++FCAVPRVL+RIY GL QK+  G F KK LFN A+  K  NM+
Sbjct: 301 RGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLFNFAFKYKHKNME 360

Query: 361 KGHNHAEASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGL 420
           KG  H +ASPI+DKIVF KVK+GLGGNVRLILSGAAPL+ H+E+FLRVVACAHVLQGYGL
Sbjct: 361 KGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRVVACAHVLQGYGL 420

Query: 421 TETCAGTFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFS 480
           TE+C GTFVS+PNELSMLGTVGPPVPNVD+ LESVPEMGYDAL++ PRGEIC+RG  LFS
Sbjct: 421 TESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPRGEICIRGKTLFS 480

Query: 481 GYYKRDDLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYG 540
           GYYKR+DLT EV IDGW HTGDVGEWQPDG++KIIDRKKNIFKLSQGEYVAVENLENIY 
Sbjct: 481 GYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGEYVAVENLENIYS 540

Query: 541 QVSSIDMIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGEL 600
            V++I+ IWVYGNS+ES+LVAV  P+ + +E WA E+ + GDF+S+C +++ K+++LGE 
Sbjct: 541 HVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICRNQKTKEFVLGEF 600

Query: 601 NRIAKEKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRI 651
           NR+AK+KKLKGFE IK +HLD +PFDMERDLITP+YK KRPQLLKYYQ  I
Sbjct: 601 NRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQKEI 651

BLAST of MS012829 vs. TAIR 10
Match: AT1G49430.1 (long-chain acyl-CoA synthetase 2 )

HSP 1 Score: 739.6 bits (1908), Expect = 2.2e-213
Identity = 348/653 (53.29%), Postives = 471/653 (72.13%), Query Frame = 0

Query: 7   LIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNRMLGRR 66
           ++ VEE + A    PS GPVYR  +AKDG       ++S W  F  +V++ P  +MLG+R
Sbjct: 8   VLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFFSEAVKKYPNEQMLGQR 67

Query: 67  EIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISMEACNAH 126
              + K G Y W+TYKE +D  I++G+++RSRG + G  CGIYGANC EWII+MEAC + 
Sbjct: 68  VTTDSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQ 127

Query: 127 GLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSFGKVTP 186
           G+  VPLYD+LG  AVEFII HAE+S+ FV+EK +  +L      +  LKTIVSFG+V+ 
Sbjct: 128 GITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSSILSCQKGCSSNLKTIVSFGEVSS 187

Query: 187 NLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVMISNKS 246
             +EE +   V +FSW+E  L+G     +LP K+++DI TIMYTSGTTG+PKGV+++N +
Sbjct: 188 TQKEEAKNQCVSLFSWNEFSLMGNLDEANLPRKRKTDICTIMYTSGTTGEPKGVILNNAA 247

Query: 247 IISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKLL 306
           I   +  + ++L+  +      +V+ SYLPLAH +D+V+E  F+  G+S+G+WRGD++ L
Sbjct: 248 ISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHCYDQVMEIYFLSRGSSVGYWRGDIRYL 307

Query: 307 IEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKGHNHAE 366
           ++D+  LKP++FC VPRV D++YAG+ QK+ +    +K LF+ AY+ K GNM+KG +  E
Sbjct: 308 MDDVQALKPTVFCGVPRVYDKLYAGIMQKISASGLIRKKLFDFAYNYKLGNMRKGFSQEE 367

Query: 367 ASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTETCAGT 426
           ASP  D+++F K+K+ LGG   ++LSGAAPL  HVE FLR++  +++ QGYGLTE+C G+
Sbjct: 368 ASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRHVEEFLRIIPASNLSQGYGLTESCGGS 427

Query: 427 FVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALST-TPRGEICVRGDPLFSGYYKRD 486
           F +L    SM+GTVG P+P V+  L SVPEMGYDA S   PRGEIC+RG+ +FSGY+KR 
Sbjct: 428 FTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYDAFSADVPRGEICLRGNSMFSGYHKRQ 487

Query: 487 DLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQVSSID 546
           DLT++V+IDGWFHTGD+GEWQ DGS+KIIDRKKNIFKLSQGEYVAVENLEN Y +   I 
Sbjct: 488 DLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKNIFKLSQGEYVAVENLENTYSRCPLIA 547

Query: 547 MIWVYGNSFESFLVAVANPNMLALERWASEN-GIEGDFDSLCEDKRAKDYLLGELNRIAK 606
            IWVYGNSFESFLV V  P+  A+E WA  N     DF+SLC++ +A+ Y L ELN  AK
Sbjct: 548 QIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQSPNDFESLCQNLKAQKYFLDELNSTAK 607

Query: 607 EKKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRIRITDLIY 658
           + +LKGFE +KAIHL+P PFD+ERDLITPT+K KRPQLL++Y+    I D +Y
Sbjct: 608 QYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLKRPQLLQHYK---GIVDQLY 657

BLAST of MS012829 vs. TAIR 10
Match: AT2G47240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 677.2 bits (1746), Expect = 1.4e-194
Identity = 320/645 (49.61%), Postives = 447/645 (69.30%), Query Frame = 0

Query: 6   YLIQVEEAKEAKDGRPSIGPVYRSSFAKDGFPPPIEGLNSCWDIFRLSVERNPGNRMLGR 65
           +  +VEE  +  DG+PS+GPVYR+  ++ GFPP    + + WDIF  SVE+ P N MLG 
Sbjct: 4   FAAKVEEGVKGIDGKPSVGPVYRNLLSEKGFPPIDSEITTAWDIFSKSVEKFPDNNMLGW 63

Query: 66  REIVNEKAGKYVWLTYKEVYDIVIKVGNSLRSRGFEEGAKCGIYGANCSEWIISMEACNA 125
           R IV+EK G Y+W TYKEVY+ V+++G++LR+ G E G++ GIYG NC +WII+MEAC A
Sbjct: 64  RRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGVNCPQWIIAMEACAA 123

Query: 126 HGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKLPEVLKTFPNTTKCLKTIVSFGKVT 185
           H L CVPLYDTLG+GAV++I+ HAEI   FV++ K+  +L+      K LK IVSF  V+
Sbjct: 124 HTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGLLEPDCKCAKRLKAIVSFTNVS 183

Query: 186 PNLREEFEKYGVGIFSWDEILLLGENQHFDLPEKKRSDISTIMYTSGTTGDPKGVMISNK 245
             L  +  + GV  +SW + L +G  +  D    K  +I TIMYTSGT+GDPKGV+++++
Sbjct: 184 DELSHKASEIGVKTYSWIDFLHMGREKPEDTNPPKAFNICTIMYTSGTSGDPKGVVLTHQ 243

Query: 246 SIISLIAGVQRLLQSVNEELTEKEVYLSYLPLAHIFDRVIEELFILHGASIGFWRGDVKL 305
           ++ + + G+   +    +++T  +VYLS+LPLAHI DR+ EE F   GAS+G++ G++ +
Sbjct: 244 AVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILDRMNEEYFFRKGASVGYYHGNLNV 303

Query: 306 LIEDIGELKPSIFCAVPRVLDRIYAGLSQKVESGSFFKKILFNLAYSRKFGNMQKGHNHA 365
           L +DI ELKP+    VPRV +RI+ G+ + ++  +  ++ +FN  Y  K   + +G++H+
Sbjct: 304 LRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPRRRFIFNALYKHKLAWLNRGYSHS 363

Query: 366 EASPISDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEAFLRVVACAHVLQGYGLTETCAG 425
           +ASP++D I F K++  LGG +RL++SG APLS  +E FLRV  C  V+QGYGLTET  G
Sbjct: 364 KASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIEEFLRVTCCCFVVQGYGLTETLGG 423

Query: 426 TFVSLPNELSMLGTVGPPVPNVDVCLESVPEMGYDALSTTPRGEICVRGDPLFSGYYKRD 485
           T +  P+E+ MLGTVG P    ++ LE V EMGYD L   P GEIC+RG  +FSGYYK  
Sbjct: 424 TALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPLGENPAGEICIRGQCMFSGYYKNP 483

Query: 486 DLTNEVMIDGWFHTGDVGEWQPDGSLKIIDRKKNIFKLSQGEYVAVENLENIYGQVSSID 545
           +LT EVM DGWFHTGD+GE  P+G LKIIDRKKN+ KLSQGEYVA+E+LENI+GQ S + 
Sbjct: 484 ELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIKLSQGEYVALEHLENIFGQNSVVQ 543

Query: 546 MIWVYGNSFESFLVAVANPNMLALERWASENGIEGDFDSLCEDKRAKDYLLGELNRIAKE 605
            IWVYG+SF+S LVAV  PN   + RWA + G    F+ LC     K++++ EL   A++
Sbjct: 544 DIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPFEELCSFPELKEHIISELKSTAEK 603

Query: 606 KKLKGFEFIKAIHLDPLPFDMERDLITPTYKKKRPQLLKYYQVRI 651
            KL+ FE+IKA+ ++  PFD+ERDL+T T K +R  LLKYYQV+I
Sbjct: 604 NKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNLLKYYQVQI 648