MS012810 (gene) Bitter gourd (TR) v1

Overview


Name	MS012810
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ethylene-responsive transcription factor CRF2-like
Location	scaffold63: 3646642 .. 3647577 (+)
RNA-Seq Expression	MS012810
Synteny	MS012810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAAATGGACTTCTTCAGTTTTGTACCTCCGATCAAGTACACTGAACATCGAAAGCAGACCAAGCTCGTCTCTGCCTCTGCCCCATTTATTGGGCCTAAGGTCGTGCGGATTTCTGTAACTGATGTCTACGCCACTGACACTGACTCTTCTAGCGGCGAGGAAGAGGAATATGGCCGCCGCCGGCGACGGCGAGTGAAGAAATTTGTGAACGAGGTTTCGATTGAGGCGTCGACGGGGAGAAATGGCCGGAAGAAGTCGACCGGGAAACAGAATTTCGCGGCGGTTACTCGGTGCCCACTGAAGCAGCTGCAGCTCGGAAGCAGAAAATTCCGCGGGGTACGGCGGCGGCCGTGGGGGAAATGGGCGGCGGAGATTAGAGATCCTCTGCGACGTGTACGGGTCTGGTTAGGGACCTACGATACGGCCGAAGAAGCGGCTATGGTCTACGACAATGCTGCCATTCAACTCCGGGGACCCCACGCGTTAACAAACTTCTCCTCCTCTCCTCCGGCAAATCCTCCGCCGGAAAACACCCCGGCGGCCAGCTCCGACTACATCTCCGCCGAGGAGTCCAACGACAATCTCTCCTCTCCCACTTCCGTTCTCCGGTGCCCCTACCCGTCAAACGACGCCGTATCCCAACAAATTCCAGCGAACGGTTCGTGCGTATCGGAGAACTTTTCCGATTTGTCGTTCTGTTCAAGCTTTGATACGTTCTTCCCCAATGATATCTTCGATTTCCAACCGCCGATTCCATCTCTGTTCGGCGGCACGGGTCTCGACGACGCCGTTTTGAAGGACGACTACGGAAGTAGCATGTTTGTAAACTCGGGCGAAGATTTCGGGTTCGGATTCTCCACGTGGCATATGGTCGAAGATTCCTTTCAGGATATCAGCGACATATTCGGGTCGGACCCTCTCGTTGCGCTG

mRNA sequence

ATGGAAGAAATGGACTTCTTCAGTTTTGTACCTCCGATCAAGTACACTGAACATCGAAAGCAGACCAAGCTCGTCTCTGCCTCTGCCCCATTTATTGGGCCTAAGGTCGTGCGGATTTCTGTAACTGATGTCTACGCCACTGACACTGACTCTTCTAGCGGCGAGGAAGAGGAATATGGCCGCCGCCGGCGACGGCGAGTGAAGAAATTTGTGAACGAGGTTTCGATTGAGGCGTCGACGGGGAGAAATGGCCGGAAGAAGTCGACCGGGAAACAGAATTTCGCGGCGGTTACTCGGTGCCCACTGAAGCAGCTGCAGCTCGGAAGCAGAAAATTCCGCGGGGTACGGCGGCGGCCGTGGGGGAAATGGGCGGCGGAGATTAGAGATCCTCTGCGACGTGTACGGGTCTGGTTAGGGACCTACGATACGGCCGAAGAAGCGGCTATGGTCTACGACAATGCTGCCATTCAACTCCGGGGACCCCACGCGTTAACAAACTTCTCCTCCTCTCCTCCGGCAAATCCTCCGCCGGAAAACACCCCGGCGGCCAGCTCCGACTACATCTCCGCCGAGGAGTCCAACGACAATCTCTCCTCTCCCACTTCCGTTCTCCGGTGCCCCTACCCGTCAAACGACGCCGTATCCCAACAAATTCCAGCGAACGGTTCGTGCGTATCGGAGAACTTTTCCGATTTGTCGTTCTGTTCAAGCTTTGATACGTTCTTCCCCAATGATATCTTCGATTTCCAACCGCCGATTCCATCTCTGTTCGGCGGCACGGGTCTCGACGACGCCGTTTTGAAGGACGACTACGGAAGTAGCATGTTTGTAAACTCGGGCGAAGATTTCGGGTTCGGATTCTCCACGTGGCATATGGTCGAAGATTCCTTTCAGGATATCAGCGACATATTCGGGTCGGACCCTCTCGTTGCGCTG

Coding sequence (CDS)

ATGGAAGAAATGGACTTCTTCAGTTTTGTACCTCCGATCAAGTACACTGAACATCGAAAGCAGACCAAGCTCGTCTCTGCCTCTGCCCCATTTATTGGGCCTAAGGTCGTGCGGATTTCTGTAACTGATGTCTACGCCACTGACACTGACTCTTCTAGCGGCGAGGAAGAGGAATATGGCCGCCGCCGGCGACGGCGAGTGAAGAAATTTGTGAACGAGGTTTCGATTGAGGCGTCGACGGGGAGAAATGGCCGGAAGAAGTCGACCGGGAAACAGAATTTCGCGGCGGTTACTCGGTGCCCACTGAAGCAGCTGCAGCTCGGAAGCAGAAAATTCCGCGGGGTACGGCGGCGGCCGTGGGGGAAATGGGCGGCGGAGATTAGAGATCCTCTGCGACGTGTACGGGTCTGGTTAGGGACCTACGATACGGCCGAAGAAGCGGCTATGGTCTACGACAATGCTGCCATTCAACTCCGGGGACCCCACGCGTTAACAAACTTCTCCTCCTCTCCTCCGGCAAATCCTCCGCCGGAAAACACCCCGGCGGCCAGCTCCGACTACATCTCCGCCGAGGAGTCCAACGACAATCTCTCCTCTCCCACTTCCGTTCTCCGGTGCCCCTACCCGTCAAACGACGCCGTATCCCAACAAATTCCAGCGAACGGTTCGTGCGTATCGGAGAACTTTTCCGATTTGTCGTTCTGTTCAAGCTTTGATACGTTCTTCCCCAATGATATCTTCGATTTCCAACCGCCGATTCCATCTCTGTTCGGCGGCACGGGTCTCGACGACGCCGTTTTGAAGGACGACTACGGAAGTAGCATGTTTGTAAACTCGGGCGAAGATTTCGGGTTCGGATTCTCCACGTGGCATATGGTCGAAGATTCCTTTCAGGATATCAGCGACATATTCGGGTCGGACCCTCTCGTTGCGCTG

Protein sequence

MEEMDFFSFVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYGRRRRRRVKKFVNEVSIEASTGRNGRKKSTGKQNFAAVTRCPLKQLQLGSRKFRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPENTPAASSDYISAEESNDNLSSPTSVLRCPYPSNDAVSQQIPANGSCVSENFSDLSFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLVAL

Homology

[Close]

BLAST of MS012810 vs. NCBI nr
Match: XP_038904776.1 (ethylene-responsive transcription factor CRF1-like [Benincasa hispida])

HSP 1 Score: 399.4 bits (1025), Expect = 2.8e-107
Identity = 231/326 (70.86%), Postives = 253/326 (77.61%), Query Frame = 0


Query: 1   MEEMDFFS--FVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEE 60
           ME+MD FS   VPPIKYTEHR QTKLV  S P +GPKVVRISVTD  ATD+ SS  E+EE
Sbjct: 1   MEQMDLFSNLVVPPIKYTEHRNQTKLV--SPPLMGPKVVRISVTDADATDS-SSDEEKEE 60

Query: 61  YGRRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVR 120
           Y     RRVKKFVNE++IEA STGR+ RKKST GK  F  V R  LKQ+  GSRKFRGVR
Sbjct: 61  Y---PCRRVKKFVNEITIEASSTGRSSRKKSTGGKSKFPTVNRGSLKQMPAGSRKFRGVR 120

Query: 121 RRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPP 180
           +RPWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP    
Sbjct: 121 QRPWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSS 180

Query: 181 PENTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFS 240
           PE   A SS Y+S EESNDNLSSPTSVLRCP PS +DAVS++I A  G  +    SE FS
Sbjct: 181 PETATAVSSGYVSTEESNDNLSSPTSVLRCPSPSAHDAVSEKISATTGKEIRRDESEKFS 240

Query: 241 DLSFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFG 300
           D SF S+ DT FPNDIFDFQPP+PS F  T  +DAVLK DYGSSMF+N G+D    FGFG
Sbjct: 241 DFSFHSNCDTIFPNDIFDFQPPVPSPFDETCFNDAVLKGDYGSSMFINPGDDFGYGFGFG 300

Query: 301 FSTWHMVEDSFQDISDIFGSDPLVAL 313
           FSTWHMVEDSFQD SDIFGSDPL+AL
Sbjct: 301 FSTWHMVEDSFQDFSDIFGSDPLLAL 319

[Close]

BLAST of MS012810 vs. NCBI nr
Match: XP_004149766.1 (ethylene-responsive transcription factor CRF2 [Cucumis sativus] >KGN53384.1 hypothetical protein Csa_015247 [Cucumis sativus])

HSP 1 Score: 395.6 bits (1015), Expect = 4.0e-106
Identity = 229/326 (70.25%), Postives = 254/326 (77.91%), Query Frame = 0


Query: 1   MEEMDFFS--FVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEE 60
           MEEMD FS   VPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EE
Sbjct: 1   MEEMDLFSTHVVPPIKYTEHRNQTRLV--SSPLMGPKVVRISVTDADATDS-SSDEEKEE 60

Query: 61  YGRRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVR 120
           Y     RRV+KFVNE++IEA STG++ RKKST GK  FAAV R  LKQ+  GSRKFRGVR
Sbjct: 61  Y---VCRRVRKFVNEITIEASSTGKSSRKKSTGGKSKFAAVNRGSLKQMPAGSRKFRGVR 120

Query: 121 RRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPP 180
           +RPWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP    
Sbjct: 121 QRPWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSS 180

Query: 181 PENTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFS 240
           PE TPA SS Y+S EESNDNLSSPTSVLRCP PS NDAVS++  A  G  +    SE FS
Sbjct: 181 PETTPAVSSGYVSTEESNDNLSSPTSVLRCPSPSANDAVSEKASATTGKEIRGEESEKFS 240

Query: 241 DLSFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFG 300
           D SF S+ DTFFPNDIFDFQ P+PS F    L+DA+LK DYGSSMF+N G+D    FGFG
Sbjct: 241 DFSFHSNCDTFFPNDIFDFQAPVPSPFEDKLLNDALLKGDYGSSMFINPGDDFEFGFGFG 300

Query: 301 FSTWHMVEDSFQDISDIFGSDPLVAL 313
            STWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 LSTWHTVEDSFLDFSDIFGSDPLLAL 319

[Close]

BLAST of MS012810 vs. NCBI nr
Match: KAA0044876.1 (ethylene-responsive transcription factor CRF2-like [Cucumis melo var. makuwa])

HSP 1 Score: 390.6 bits (1002), Expect = 1.3e-104
Identity = 228/324 (70.37%), Postives = 252/324 (77.78%), Query Frame = 0


Query: 1   MEEMDFFSFVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYG 60
           MEEMDF  FVPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EEY 
Sbjct: 1   MEEMDF--FVPPIKYTEHRNQTRLV--SSPSMGPKVVRISVTDADATDS-SSDEEKEEY- 60

Query: 61  RRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRRR 120
               RRVKKFVNE++IEA STG++ RKKST GK  FAAV R  LKQ+  GSRKFRGVR+R
Sbjct: 61  --VCRRVKKFVNEITIEASSTGKSSRKKSTGGKSKFAAVNRGSLKQMPAGSRKFRGVRQR 120

Query: 121 PWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPE 180
           PWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP     E
Sbjct: 121 PWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSSTE 180

Query: 181 NTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFSDL 240
            T A SS Y S EESNDNLSSPTSVLRC  PS NDAVS+++ A  G  +    SE FSD 
Sbjct: 181 TTTAVSSGYASTEESNDNLSSPTSVLRCLSPSANDAVSEKVSATTGKEIRGDESEKFSDF 240

Query: 241 SFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFGFS 300
           SF S+ DTFFPNDIFDFQPP+PS F    L+DA+LK DYGSSMF+N G+D    FGFG S
Sbjct: 241 SFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYGSSMFINPGDDFEFGFGFGLS 300

Query: 301 TWHMVEDSFQDISDIFGSDPLVAL 313
           TWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 TWHTVEDSFLDFSDIFGSDPLLAL 315

[Close]

BLAST of MS012810 vs. NCBI nr
Match: XP_008451973.1 (PREDICTED: ethylene-responsive transcription factor CRF2-like [Cucumis melo] >TYK16594.1 ethylene-responsive transcription factor CRF2-like [Cucumis melo var. makuwa])

HSP 1 Score: 388.7 bits (997), Expect = 4.9e-104
Identity = 226/324 (69.75%), Postives = 252/324 (77.78%), Query Frame = 0


Query: 1   MEEMDFFSFVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYG 60
           MEEMDF  FVPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EEY 
Sbjct: 1   MEEMDF--FVPPIKYTEHRNQTRLV--SSPLMGPKVVRISVTDADATDS-SSDEEKEEY- 60

Query: 61  RRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRRR 120
               RRVKKFVNE++IEA STG++ RKKST GK  FA+V R  LKQ+  GSRKFRGVR+R
Sbjct: 61  --VCRRVKKFVNEITIEASSTGKSSRKKSTGGKSKFASVNRGSLKQMPAGSRKFRGVRQR 120

Query: 121 PWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPE 180
           PWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP     E
Sbjct: 121 PWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSSTE 180

Query: 181 NTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFSDL 240
            T A SS Y S EESN+NLSSPTSVLRC  PS NDAVS+++ A  G  +    SE FSD 
Sbjct: 181 TTTAVSSGYASTEESNENLSSPTSVLRCLSPSANDAVSEKVSATTGKEIRGDESEKFSDF 240

Query: 241 SFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFGFS 300
           SF S+ DTFFPNDIFDFQPP+PS F    L+DA+LK DYGSSMF+N G+D    FGFG S
Sbjct: 241 SFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYGSSMFINPGDDFEFGFGFGLS 300

Query: 301 TWHMVEDSFQDISDIFGSDPLVAL 313
           TWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 TWHTVEDSFLDFSDIFGSDPLLAL 315

[Close]

BLAST of MS012810 vs. NCBI nr
Match: XP_022931312.1 (ethylene-responsive transcription factor CRF2-like [Cucurbita moschata])

HSP 1 Score: 379.0 bits (972), Expect = 3.9e-101
Identity = 216/326 (66.26%), Postives = 246/326 (75.46%), Query Frame = 0


Query: 1   MEEMDFFS--FVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEE 60
           ME MD FS   +PPIKYTEHR QT+LV  S P +  KVV+ISV D  ATD+ SS  EE++
Sbjct: 1   MEPMDLFSSPVLPPIKYTEHRSQTRLV--SPPLMTSKVVKISVMDADATDS-SSDDEEQD 60

Query: 61  YGRRRRRRVKKFVNEVSIEASTGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRR 120
           Y     RRVKKFVNE++IEAS  ++ RKKST GK  F AV R PLKQ   G++KFRGVR+
Sbjct: 61  YA---CRRVKKFVNEITIEASPVKSSRKKSTGGKSKFPAVNRDPLKQQPTGAKKFRGVRQ 120

Query: 121 RPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPP 180
           RPWGKWAAEIRDPLRRVRVWLGTY+TAEEAAMVYDNAAIQLRGP A TNF   P  +P  
Sbjct: 121 RPWGKWAAEIRDPLRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFIPPPKFSP-- 180

Query: 181 ENTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA------NGSCVSENFS 240
             T AASS Y+SAEESN+NLSSPTSVLRCP PS NDAVS +         NG    +  S
Sbjct: 181 -ETAAASSGYVSAEESNENLSSPTSVLRCPSPSTNDAVSGKTSTTTTGKENGRDEPDKIS 240

Query: 241 DLSFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGEDFGFGF--- 300
           + SF S++DTFFPNDIFDF+PP+PSLFG T L+D  LK DYGSSMF+N  +DFGFGF   
Sbjct: 241 EFSFHSNYDTFFPNDIFDFRPPVPSLFGETTLNDGFLKGDYGSSMFINPADDFGFGFGFG 300

Query: 301 -STWHMVEDSFQDISDIFGSDPLVAL 313
            STWH V+DSFQDISDIFGSDPL+AL
Sbjct: 301 LSTWHTVKDSFQDISDIFGSDPLLAL 317

[Close]

BLAST of MS012810 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.2e-58
Identity = 157/343 (45.77%), Postives = 200/343 (58.31%), Query Frame = 0


Query: 10  VPPIKYTEHR-KQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYGR--RRRRR 69
           +P IK+TEH+   T +VS        +++RISVTD  ATD+ SS  EEEE+ R   +RRR
Sbjct: 9   LPRIKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDS-SSDDEEEEHQRFVSKRRR 68

Query: 70  VKKFVNEVSIEAS---TGRNGRKKSTGKQNFAAVTRCPLKQL------QLGSRKFRGVRR 129
           VKKFVNEV +++    TG  G+ +S  +Q  A  +   +  +        G +KFRGVR+
Sbjct: 69  VKKFVNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQ 128

Query: 130 RPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPA---- 189
           RPWGKWAAEIRDPL+RVR+WLGTY+TAEEAAMVYDNAAIQLRGP ALTNFS +P      
Sbjct: 129 RPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEK 188

Query: 190 -NPPP------------ENTPAASSDYISAEESNDNLSSPTSVLRCPYPSND-------- 249
             PPP                  +S  IS   SND L SP SVLR P+  ++        
Sbjct: 189 KAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSSP 248

Query: 250 --AVSQQIPANGSCVSENFSDLSFCSSFDTFFPNDIFDFQPP-IPSLFGGTGLDDAVLKD 309
             AV  +   + + VSE FSD     S   F  +D+FDF+   +P   GG    + +   
Sbjct: 249 VAAVVVKEEPSMTTVSETFSDF----SAPLFSDDDVFDFRSSVVPDYLGGDLFGEDLFTA 308

Query: 310 DYGSSMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLVAL 313
           D  + M  N G DFG G S+WHM ED FQDI D+FGSDPL+A+
Sbjct: 309 DMCTDM--NFGFDFGSGLSSWHM-EDHFQDIGDLFGSDPLLAV 343

[Close]

BLAST of MS012810 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.4e-47
Identity = 128/293 (43.69%), Postives = 172/293 (58.70%), Query Frame = 0


Query: 34  PKVVRISVTDVYATDTDSSSGEEEEYG--RRRRRRVKKFVNEVSIEASTGRNGRKKSTGK 93
           P+++RISVTD YATD+ S   EE ++     +RRRVKK+V EV +++    + ++K   K
Sbjct: 10  PRILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVV--SDKEKPMKK 69

Query: 94  QNFAAVTRCPLKQLQLGSRKFRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVY 153
           +    V   P+  +   +RKFRGVR+RPWGKWAAEIRDP RRVRVWLGT+DTAEEAA+VY
Sbjct: 70  KRKKRVVTVPV-VVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVY 129

Query: 154 DNAAIQLRGPHALTNFSSSPPANPPPENTPAASSDYISAEE----SNDNLSSPTSVLRCP 213
           DNAAIQLRGP+A  NF    P  P  EN   AS++     +      + L SP SVL  P
Sbjct: 130 DNAAIQLRGPNAELNF----PPPPVTENVEEASTEVKGVSDFIIGGGECLRSPVSVLESP 189

Query: 214 YPSNDAVSQQIPANGSCVSE-------NFSDLSFCSSFDTFFPNDIFDFQPPIPSLFGGT 273
           + S ++ + +    G   +E       +F+   F  S   F  +D+F F   +   FGG 
Sbjct: 190 F-SGESTAVKEEFVGVSTAEIVVKKEPSFNGSDF--SAPLFSDDDVFGFSTSMSESFGGD 249

Query: 274 GLDDAVLKD-DYGSSMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLVAL 313
              D +  D  +GS     SG     GFS+WH VED FQDI D+FGSDP++ +
Sbjct: 250 LFGDNLFADMSFGSGFGFGSGS----GFSSWH-VEDHFQDIGDLFGSDPVLTV 287

[Close]

BLAST of MS012810 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.0e-35
Identity = 134/357 (37.54%), Postives = 175/357 (49.02%), Query Frame = 0


Query: 4   MDFFSFVPPIKYTEHR-KQTKLVSASAPFI--GPKVVRISVTDVYATDTDSSSGEEEEYG 63
           MD +    P+KYTEH+   TK    S+  +  G  + ++S+       TDSSS E+EE  
Sbjct: 1   MDEYIDFRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDF 60

Query: 64  RRRRRRVKKFVNEVSIEAS-------TGRNGRKK----STGKQNFAAVTRCPLKQLQLGS 123
              RRRVK+FVNE+++E S            RK+    S   Q+ A+  + P  ++ +  
Sbjct: 61  LFPRRRVKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSG 120

Query: 124 --RKFRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNF 183
             +KFRGVR+RPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGP ALTNF
Sbjct: 121 QIKKFRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNF 180

Query: 184 SSSPPANPPPEN---------------TPAASSDYISAEESNDNLSSPTSVL-------- 243
           S  P      E                TP  SS   S EE   +LSSPTSVL        
Sbjct: 181 SIPPQEEEEEEEPEPVIEEKPVIMTTPTPTTSSSE-STEEDLQHLSSPTSVLNHRSEEIQ 240

Query: 244 RCPYPSNDAVSQQIPANGSCVSENF------SDLSFCSSFDTFFPNDIFDFQPPIPSLFG 303
           +   P   A  +   +N       F      SD SF    DT F ++ F+  PP  S+F 
Sbjct: 241 QVQQPFKSAKPEPGVSNAPWWHTGFNTGLGESDDSF--PLDTPFLDNYFNESPPEMSIFD 300

Query: 304 GTGLDDAVLKDDYGSSMFVNSGEDFGFGFS-TWHMVED---SFQDISDIFGSDPLVA 312
                     DD  + M    GE        T   ++D   +F D  D   SD LVA
Sbjct: 301 QPMDQIFCENDDIFNDMLFLGGETMNIEDELTSSSIKDMGSTFSDFDDSLISDLLVA 354

[Close]

BLAST of MS012810 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 3.3e-34
Identity = 122/337 (36.20%), Postives = 175/337 (51.93%), Query Frame = 0


Query: 4   MDFFSFVPPIKYTEHR-------KQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEE 63
           MD F  + P+KYTEH+       K++ +   ++     ++VR+S+TD  ATD   SS +E
Sbjct: 3   MDEFMDLRPVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATD---SSSDE 62

Query: 64  EEYGRRRRRRVKKFVNEVSIEASTGRNG-----RKKSTGKQNFAAVTRCPLKQLQLGSRK 123
           EE+    RRRVK+ +NE+ +E S+   G       K   + N  +  + P    Q   +K
Sbjct: 63  EEF-LFPRRRVKRLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVSGQ-NQKK 122

Query: 124 FRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSP 183
           +RGVR+RPWGKWAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGP ALTNF+  P
Sbjct: 123 YRGVRQRPWGKWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQP 182

Query: 184 PANPPPENTPAASSDY-ISAEESND---NLSSPTSVLRC-PYPSNDAVSQQI-PANGSCV 243
              P  E      S+  +S  ES D   +LSSPTSVL    Y S + +   I P     +
Sbjct: 183 EPEPVQEQEQEPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFL 242

Query: 244 SENFSDLSF------CSSFDTFFPNDI------FDFQPPIPSLFGGTGLDDAVLKDDYGS 303
                 +S+       ++ D  FP DI      F+   P  S+F          ++D+ +
Sbjct: 243 EPEQEPISWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIFDQPMSPIQPTENDFFN 302

Query: 304 SMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLV 311
            + +       +  S    +  SF D+ D   SD L+
Sbjct: 303 DLMLFDSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

[Close]

BLAST of MS012810 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.1e-29
Identity = 105/292 (35.96%), Postives = 152/292 (52.05%), Query Frame = 0


Query: 13  IKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYGRRRRRRVKKFVN 72
           +K+TE+R  T  V+A+     P++VRI+VTD +A  TDSSS +++        RVK++V 
Sbjct: 8   VKFTENRTVTN-VAATPSNGSPRLVRITVTDPFA--TDSSSDDDDNNNVTVVPRVKRYVK 67

Query: 73  EVSI--EASTGRNGRKKSTGKQNFAAVTRCPLKQLQLGSRKFRGVRRRPWGKWAAEIRDP 132
           E+      S+     +K   K+  + V    +    +  +K+RGVR+RPWGK+AAEIRDP
Sbjct: 68  EIRFCQGESSSSTAARKGKHKEEESVVVEDDV-STSVKPKKYRGVRQRPWGKFAAEIRDP 127

Query: 133 LRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPENTPAASSDYISA 192
             R R+WLGT+ TAEEAA+ YD AAI L+GP ALTNF + P   P  +    ++ DY   
Sbjct: 128 SSRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTVSACDY--G 187

Query: 193 EESNDNLSSPTSVLRCPYPSNDAVSQQI------PANGSCV----SENFSDLSFCSSF-- 252
            +S  +L SPTSVLR  +  N+    +I      P   S V     E+ + L F   +  
Sbjct: 188 RDSRQSLHSPTSVLR--FNVNEETEHEIEAIELSPERKSTVIKEEEESSAGLVFPDPYLL 247

Query: 253 -DTFFPNDIF---DFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGEDFGFG 287
            D     + F   +  P +  L   T +   +L +   S    N  EDF FG
Sbjct: 248 PDLSLAGECFWDTEIAPDLLFLDEETKIQSTLLPNTEVSKQGENETEDFEFG 291

[Close]

BLAST of MS012810 vs. ExPASy TrEMBL
Match: A0A0A0KWY5 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051360 PE=4 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.9e-106
Identity = 229/326 (70.25%), Postives = 254/326 (77.91%), Query Frame = 0


Query: 1   MEEMDFFS--FVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEE 60
           MEEMD FS   VPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EE
Sbjct: 1   MEEMDLFSTHVVPPIKYTEHRNQTRLV--SSPLMGPKVVRISVTDADATDS-SSDEEKEE 60

Query: 61  YGRRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVR 120
           Y     RRV+KFVNE++IEA STG++ RKKST GK  FAAV R  LKQ+  GSRKFRGVR
Sbjct: 61  Y---VCRRVRKFVNEITIEASSTGKSSRKKSTGGKSKFAAVNRGSLKQMPAGSRKFRGVR 120

Query: 121 RRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPP 180
           +RPWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP    
Sbjct: 121 QRPWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSS 180

Query: 181 PENTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFS 240
           PE TPA SS Y+S EESNDNLSSPTSVLRCP PS NDAVS++  A  G  +    SE FS
Sbjct: 181 PETTPAVSSGYVSTEESNDNLSSPTSVLRCPSPSANDAVSEKASATTGKEIRGEESEKFS 240

Query: 241 DLSFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFG 300
           D SF S+ DTFFPNDIFDFQ P+PS F    L+DA+LK DYGSSMF+N G+D    FGFG
Sbjct: 241 DFSFHSNCDTFFPNDIFDFQAPVPSPFEDKLLNDALLKGDYGSSMFINPGDDFEFGFGFG 300

Query: 301 FSTWHMVEDSFQDISDIFGSDPLVAL 313
            STWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 LSTWHTVEDSFLDFSDIFGSDPLLAL 319

[Close]

BLAST of MS012810 vs. ExPASy TrEMBL
Match: A0A5A7TRV9 (Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001690 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 6.2e-105
Identity = 228/324 (70.37%), Postives = 252/324 (77.78%), Query Frame = 0


Query: 1   MEEMDFFSFVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYG 60
           MEEMDF  FVPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EEY 
Sbjct: 1   MEEMDF--FVPPIKYTEHRNQTRLV--SSPSMGPKVVRISVTDADATDS-SSDEEKEEY- 60

Query: 61  RRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRRR 120
               RRVKKFVNE++IEA STG++ RKKST GK  FAAV R  LKQ+  GSRKFRGVR+R
Sbjct: 61  --VCRRVKKFVNEITIEASSTGKSSRKKSTGGKSKFAAVNRGSLKQMPAGSRKFRGVRQR 120

Query: 121 PWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPE 180
           PWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP     E
Sbjct: 121 PWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSSTE 180

Query: 181 NTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFSDL 240
            T A SS Y S EESNDNLSSPTSVLRC  PS NDAVS+++ A  G  +    SE FSD 
Sbjct: 181 TTTAVSSGYASTEESNDNLSSPTSVLRCLSPSANDAVSEKVSATTGKEIRGDESEKFSDF 240

Query: 241 SFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFGFS 300
           SF S+ DTFFPNDIFDFQPP+PS F    L+DA+LK DYGSSMF+N G+D    FGFG S
Sbjct: 241 SFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYGSSMFINPGDDFEFGFGFGLS 300

Query: 301 TWHMVEDSFQDISDIFGSDPLVAL 313
           TWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 TWHTVEDSFLDFSDIFGSDPLLAL 315

[Close]

BLAST of MS012810 vs. ExPASy TrEMBL
Match: A0A5D3D045 (Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004170 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 2.4e-104
Identity = 226/324 (69.75%), Postives = 252/324 (77.78%), Query Frame = 0


Query: 1   MEEMDFFSFVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYG 60
           MEEMDF  FVPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EEY 
Sbjct: 1   MEEMDF--FVPPIKYTEHRNQTRLV--SSPLMGPKVVRISVTDADATDS-SSDEEKEEY- 60

Query: 61  RRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRRR 120
               RRVKKFVNE++IEA STG++ RKKST GK  FA+V R  LKQ+  GSRKFRGVR+R
Sbjct: 61  --VCRRVKKFVNEITIEASSTGKSSRKKSTGGKSKFASVNRGSLKQMPAGSRKFRGVRQR 120

Query: 121 PWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPE 180
           PWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP     E
Sbjct: 121 PWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSSTE 180

Query: 181 NTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFSDL 240
            T A SS Y S EESN+NLSSPTSVLRC  PS NDAVS+++ A  G  +    SE FSD 
Sbjct: 181 TTTAVSSGYASTEESNENLSSPTSVLRCLSPSANDAVSEKVSATTGKEIRGDESEKFSDF 240

Query: 241 SFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFGFS 300
           SF S+ DTFFPNDIFDFQPP+PS F    L+DA+LK DYGSSMF+N G+D    FGFG S
Sbjct: 241 SFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYGSSMFINPGDDFEFGFGFGLS 300

Query: 301 TWHMVEDSFQDISDIFGSDPLVAL 313
           TWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 TWHTVEDSFLDFSDIFGSDPLLAL 315

[Close]

BLAST of MS012810 vs. ExPASy TrEMBL
Match: A0A1S3BST1 (ethylene-responsive transcription factor CRF2-like OS=Cucumis melo OX=3656 GN=LOC103493117 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 2.4e-104
Identity = 226/324 (69.75%), Postives = 252/324 (77.78%), Query Frame = 0


Query: 1   MEEMDFFSFVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYG 60
           MEEMDF  FVPPIKYTEHR QT+LV  S+P +GPKVVRISVTD  ATD+ SS  E+EEY 
Sbjct: 1   MEEMDF--FVPPIKYTEHRNQTRLV--SSPLMGPKVVRISVTDADATDS-SSDEEKEEY- 60

Query: 61  RRRRRRVKKFVNEVSIEA-STGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRRR 120
               RRVKKFVNE++IEA STG++ RKKST GK  FA+V R  LKQ+  GSRKFRGVR+R
Sbjct: 61  --VCRRVKKFVNEITIEASSTGKSSRKKSTGGKSKFASVNRGSLKQMPAGSRKFRGVRQR 120

Query: 121 PWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPPE 180
           PWGKWAAEIRDP RRVRVWLGTY+TAEEAAMVYDNAAIQLRGP ALTNF + PP     E
Sbjct: 121 PWGKWAAEIRDPSRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTALTNF-TPPPVKSSTE 180

Query: 181 NTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA-NGSCV----SENFSDL 240
            T A SS Y S EESN+NLSSPTSVLRC  PS NDAVS+++ A  G  +    SE FSD 
Sbjct: 181 TTTAVSSGYASTEESNENLSSPTSVLRCLSPSANDAVSEKVSATTGKEIRGDESEKFSDF 240

Query: 241 SFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGED----FGFGFS 300
           SF S+ DTFFPNDIFDFQPP+PS F    L+DA+LK DYGSSMF+N G+D    FGFG S
Sbjct: 241 SFHSNCDTFFPNDIFDFQPPVPSPFDDKLLNDALLKGDYGSSMFINPGDDFEFGFGFGLS 300

Query: 301 TWHMVEDSFQDISDIFGSDPLVAL 313
           TWH VEDSF D SDIFGSDPL+AL
Sbjct: 301 TWHTVEDSFLDFSDIFGSDPLLAL 315

[Close]

BLAST of MS012810 vs. ExPASy TrEMBL
Match: A0A6J1ETA3 (ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662 GN=LOC111437533 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.9e-101
Identity = 216/326 (66.26%), Postives = 246/326 (75.46%), Query Frame = 0


Query: 1   MEEMDFFS--FVPPIKYTEHRKQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEE 60
           ME MD FS   +PPIKYTEHR QT+LV  S P +  KVV+ISV D  ATD+ SS  EE++
Sbjct: 1   MEPMDLFSSPVLPPIKYTEHRSQTRLV--SPPLMTSKVVKISVMDADATDS-SSDDEEQD 60

Query: 61  YGRRRRRRVKKFVNEVSIEASTGRNGRKKST-GKQNFAAVTRCPLKQLQLGSRKFRGVRR 120
           Y     RRVKKFVNE++IEAS  ++ RKKST GK  F AV R PLKQ   G++KFRGVR+
Sbjct: 61  YA---CRRVKKFVNEITIEASPVKSSRKKSTGGKSKFPAVNRDPLKQQPTGAKKFRGVRQ 120

Query: 121 RPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPANPPP 180
           RPWGKWAAEIRDPLRRVRVWLGTY+TAEEAAMVYDNAAIQLRGP A TNF   P  +P  
Sbjct: 121 RPWGKWAAEIRDPLRRVRVWLGTYNTAEEAAMVYDNAAIQLRGPTAQTNFIPPPKFSP-- 180

Query: 181 ENTPAASSDYISAEESNDNLSSPTSVLRCPYPS-NDAVSQQIPA------NGSCVSENFS 240
             T AASS Y+SAEESN+NLSSPTSVLRCP PS NDAVS +         NG    +  S
Sbjct: 181 -ETAAASSGYVSAEESNENLSSPTSVLRCPSPSTNDAVSGKTSTTTTGKENGRDEPDKIS 240

Query: 241 DLSFCSSFDTFFPNDIFDFQPPIPSLFGGTGLDDAVLKDDYGSSMFVNSGEDFGFGF--- 300
           + SF S++DTFFPNDIFDF+PP+PSLFG T L+D  LK DYGSSMF+N  +DFGFGF   
Sbjct: 241 EFSFHSNYDTFFPNDIFDFRPPVPSLFGETTLNDGFLKGDYGSSMFINPADDFGFGFGFG 300

Query: 301 -STWHMVEDSFQDISDIFGSDPLVAL 313
            STWH V+DSFQDISDIFGSDPL+AL
Sbjct: 301 LSTWHTVKDSFQDISDIFGSDPLLAL 317

[Close]

BLAST of MS012810 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 225.7 bits (574), Expect = 5.1e-59
Identity = 157/343 (45.77%), Postives = 200/343 (58.31%), Query Frame = 0


Query: 10  VPPIKYTEHR-KQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYGR--RRRRR 69
           +P IK+TEH+   T +VS        +++RISVTD  ATD+ SS  EEEE+ R   +RRR
Sbjct: 9   LPRIKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDS-SSDDEEEEHQRFVSKRRR 68

Query: 70  VKKFVNEVSIEAS---TGRNGRKKSTGKQNFAAVTRCPLKQL------QLGSRKFRGVRR 129
           VKKFVNEV +++    TG  G+ +S  +Q  A  +   +  +        G +KFRGVR+
Sbjct: 69  VKKFVNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQ 128

Query: 130 RPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPA---- 189
           RPWGKWAAEIRDPL+RVR+WLGTY+TAEEAAMVYDNAAIQLRGP ALTNFS +P      
Sbjct: 129 RPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEK 188

Query: 190 -NPPP------------ENTPAASSDYISAEESNDNLSSPTSVLRCPYPSND-------- 249
             PPP                  +S  IS   SND L SP SVLR P+  ++        
Sbjct: 189 KAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSSP 248

Query: 250 --AVSQQIPANGSCVSENFSDLSFCSSFDTFFPNDIFDFQPP-IPSLFGGTGLDDAVLKD 309
             AV  +   + + VSE FSD     S   F  +D+FDF+   +P   GG    + +   
Sbjct: 249 VAAVVVKEEPSMTTVSETFSDF----SAPLFSDDDVFDFRSSVVPDYLGGDLFGEDLFTA 308

Query: 310 DYGSSMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLVAL 313
           D  + M  N G DFG G S+WHM ED FQDI D+FGSDPL+A+
Sbjct: 309 DMCTDM--NFGFDFGSGLSSWHM-EDHFQDIGDLFGSDPLLAV 343

[Close]

BLAST of MS012810 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 225.7 bits (574), Expect = 5.1e-59
Identity = 157/343 (45.77%), Postives = 200/343 (58.31%), Query Frame = 0


Query: 10  VPPIKYTEHR-KQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEEEEYGR--RRRRR 69
           +P IK+TEH+   T +VS        +++RISVTD  ATD+ SS  EEEE+ R   +RRR
Sbjct: 9   LPRIKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDS-SSDDEEEEHQRFVSKRRR 68

Query: 70  VKKFVNEVSIEAS---TGRNGRKKSTGKQNFAAVTRCPLKQL------QLGSRKFRGVRR 129
           VKKFVNEV +++    TG  G+ +S  +Q  A  +   +  +        G +KFRGVR+
Sbjct: 69  VKKFVNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQ 128

Query: 130 RPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSPPA---- 189
           RPWGKWAAEIRDPL+RVR+WLGTY+TAEEAAMVYDNAAIQLRGP ALTNFS +P      
Sbjct: 129 RPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEK 188

Query: 190 -NPPP------------ENTPAASSDYISAEESNDNLSSPTSVLRCPYPSND-------- 249
             PPP                  +S  IS   SND L SP SVLR P+  ++        
Sbjct: 189 KAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGISSSP 248

Query: 250 --AVSQQIPANGSCVSENFSDLSFCSSFDTFFPNDIFDFQPP-IPSLFGGTGLDDAVLKD 309
             AV  +   + + VSE FSD     S   F  +D+FDF+   +P   GG    + +   
Sbjct: 249 VAAVVVKEEPSMTTVSETFSDF----SAPLFSDDDVFDFRSSVVPDYLGGDLFGEDLFTA 308

Query: 310 DYGSSMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLVAL 313
           D  + M  N G DFG G S+WHM ED FQDI D+FGSDPL+A+
Sbjct: 309 DMCTDM--NFGFDFGSGLSSWHM-EDHFQDIGDLFGSDPLLAV 343

[Close]

BLAST of MS012810 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-48
Identity = 128/293 (43.69%), Postives = 172/293 (58.70%), Query Frame = 0


Query: 34  PKVVRISVTDVYATDTDSSSGEEEEYG--RRRRRRVKKFVNEVSIEASTGRNGRKKSTGK 93
           P+++RISVTD YATD+ S   EE ++     +RRRVKK+V EV +++    + ++K   K
Sbjct: 10  PRILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVV--SDKEKPMKK 69

Query: 94  QNFAAVTRCPLKQLQLGSRKFRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVY 153
           +    V   P+  +   +RKFRGVR+RPWGKWAAEIRDP RRVRVWLGT+DTAEEAA+VY
Sbjct: 70  KRKKRVVTVPV-VVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVY 129

Query: 154 DNAAIQLRGPHALTNFSSSPPANPPPENTPAASSDYISAEE----SNDNLSSPTSVLRCP 213
           DNAAIQLRGP+A  NF    P  P  EN   AS++     +      + L SP SVL  P
Sbjct: 130 DNAAIQLRGPNAELNF----PPPPVTENVEEASTEVKGVSDFIIGGGECLRSPVSVLESP 189

Query: 214 YPSNDAVSQQIPANGSCVSE-------NFSDLSFCSSFDTFFPNDIFDFQPPIPSLFGGT 273
           + S ++ + +    G   +E       +F+   F  S   F  +D+F F   +   FGG 
Sbjct: 190 F-SGESTAVKEEFVGVSTAEIVVKKEPSFNGSDF--SAPLFSDDDVFGFSTSMSESFGGD 249

Query: 274 GLDDAVLKD-DYGSSMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLVAL 313
              D +  D  +GS     SG     GFS+WH VED FQDI D+FGSDP++ +
Sbjct: 250 LFGDNLFADMSFGSGFGFGSGS----GFSSWH-VEDHFQDIGDLFGSDPVLTV 287

[Close]

BLAST of MS012810 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 149.8 bits (377), Expect = 3.6e-36
Identity = 134/357 (37.54%), Postives = 175/357 (49.02%), Query Frame = 0


Query: 4   MDFFSFVPPIKYTEHR-KQTKLVSASAPFI--GPKVVRISVTDVYATDTDSSSGEEEEYG 63
           MD +    P+KYTEH+   TK    S+  +  G  + ++S+       TDSSS E+EE  
Sbjct: 1   MDEYIDFRPLKYTEHKTSMTKYTKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDF 60

Query: 64  RRRRRRVKKFVNEVSIEAS-------TGRNGRKK----STGKQNFAAVTRCPLKQLQLGS 123
              RRRVK+FVNE+++E S            RK+    S   Q+ A+  + P  ++ +  
Sbjct: 61  LFPRRRVKRFVNEITVEPSCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSG 120

Query: 124 --RKFRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNF 183
             +KFRGVR+RPWGKWAAEIRDP +R R+WLGT++TAEEAA+VYDNAAI+LRGP ALTNF
Sbjct: 121 QIKKFRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNF 180

Query: 184 SSSPPANPPPEN---------------TPAASSDYISAEESNDNLSSPTSVL-------- 243
           S  P      E                TP  SS   S EE   +LSSPTSVL        
Sbjct: 181 SIPPQEEEEEEEPEPVIEEKPVIMTTPTPTTSSSE-STEEDLQHLSSPTSVLNHRSEEIQ 240

Query: 244 RCPYPSNDAVSQQIPANGSCVSENF------SDLSFCSSFDTFFPNDIFDFQPPIPSLFG 303
           +   P   A  +   +N       F      SD SF    DT F ++ F+  PP  S+F 
Sbjct: 241 QVQQPFKSAKPEPGVSNAPWWHTGFNTGLGESDDSF--PLDTPFLDNYFNESPPEMSIFD 300

Query: 304 GTGLDDAVLKDDYGSSMFVNSGEDFGFGFS-TWHMVED---SFQDISDIFGSDPLVA 312
                     DD  + M    GE        T   ++D   +F D  D   SD LVA
Sbjct: 301 QPMDQIFCENDDIFNDMLFLGGETMNIEDELTSSSIKDMGSTFSDFDDSLISDLLVA 354

[Close]

BLAST of MS012810 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 147.1 bits (370), Expect = 2.3e-35
Identity = 122/337 (36.20%), Postives = 175/337 (51.93%), Query Frame = 0


Query: 4   MDFFSFVPPIKYTEHR-------KQTKLVSASAPFIGPKVVRISVTDVYATDTDSSSGEE 63
           MD F  + P+KYTEH+       K++ +   ++     ++VR+S+TD  ATD   SS +E
Sbjct: 3   MDEFMDLRPVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATD---SSSDE 62

Query: 64  EEYGRRRRRRVKKFVNEVSIEASTGRNG-----RKKSTGKQNFAAVTRCPLKQLQLGSRK 123
           EE+    RRRVK+ +NE+ +E S+   G       K   + N  +  + P    Q   +K
Sbjct: 63  EEF-LFPRRRVKRLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKPSVSGQ-NQKK 122

Query: 124 FRGVRRRPWGKWAAEIRDPLRRVRVWLGTYDTAEEAAMVYDNAAIQLRGPHALTNFSSSP 183
           +RGVR+RPWGKWAAEIRDP +R R+WLGT+ TAEEAA+VYDNAAI+LRGP ALTNF+  P
Sbjct: 123 YRGVRQRPWGKWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQP 182

Query: 184 PANPPPENTPAASSDY-ISAEESND---NLSSPTSVLRC-PYPSNDAVSQQI-PANGSCV 243
              P  E      S+  +S  ES D   +LSSPTSVL    Y S + +   I P     +
Sbjct: 183 EPEPVQEQEQEPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFL 242

Query: 244 SENFSDLSF------CSSFDTFFPNDI------FDFQPPIPSLFGGTGLDDAVLKDDYGS 303
                 +S+       ++ D  FP DI      F+   P  S+F          ++D+ +
Sbjct: 243 EPEQEPISWHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIFDQPMSPIQPTENDFFN 302

Query: 304 SMFVNSGEDFGFGFSTWHMVEDSFQDISDIFGSDPLV 311
            + +       +  S    +  SF D+ D   SD L+
Sbjct: 303 DLMLFDSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

The following BLAST results are available for this feature:

BLAST of MS012810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904776.1	2.8e-107	70.86	ethylene-responsive transcription factor CRF1-like [Benincasa hispida]	[more]
XP_004149766.1	4.0e-106	70.25	ethylene-responsive transcription factor CRF2 [Cucumis sativus] >KGN53384.1 hypo...	[more]
KAA0044876.1	1.3e-104	70.37	ethylene-responsive transcription factor CRF2-like [Cucumis melo var. makuwa]	[more]
XP_008451973.1	4.9e-104	69.75	PREDICTED: ethylene-responsive transcription factor CRF2-like [Cucumis melo] >TY...	[more]
XP_022931312.1	3.9e-101	66.26	ethylene-responsive transcription factor CRF2-like [Cucurbita moschata]	[more]

BLAST of MS012810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUQ2	7.2e-58	45.77	Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O82503	3.4e-47	43.69	Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9FK12	5.0e-35	37.54	Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9SUE3	3.3e-34	36.20	Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9M374	4.1e-29	35.96	Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of MS012810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWY5	1.9e-106	70.25	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G051360 PE=...	[more]
A0A5A7TRV9	6.2e-105	70.37	Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa O...	[more]
A0A5D3D045	2.4e-104	69.75	Ethylene-responsive transcription factor CRF2-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BST1	2.4e-104	69.75	ethylene-responsive transcription factor CRF2-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1ETA3	1.9e-101	66.26	ethylene-responsive transcription factor CRF2-like OS=Cucurbita moschata OX=3662...	[more]

BLAST of MS012810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23750.1	5.1e-59	45.77	cytokinin response factor 2	[more]
AT4G23750.2	5.1e-59	45.77	cytokinin response factor 2	[more]
AT4G11140.1	2.4e-48	43.69	cytokinin response factor 1	[more]
AT5G53290.1	3.6e-36	37.54	cytokinin response factor 3	[more]
AT4G27950.1	2.3e-35	36.20	cytokinin response factor 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 112..123 score: 69.39 coord: 134..150 score: 63.96
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 111..174 e-value: 3.5E-37 score: 139.5
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 111..160 e-value: 4.5E-14 score: 52.4
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 111..168 score: 23.656738
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 111..167 e-value: 2.08622E-32 score: 112.75
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 110..169 e-value: 6.9E-26 score: 92.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..202
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 163..202
None	No IPR available	PANTHER	PTHR31194:SF152	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF5-RELATED	coord: 10..308
None	No IPR available	PANTHER	PTHR31194	SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR	coord: 10..308
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 111..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012810.1	MS012810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity