MS012775 (gene) Bitter gourd (TR) v1

Overview
NameMS012775
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold63: 3346457 .. 3346654 (-)
RNA-Seq ExpressionMS012775
SyntenyMS012775
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAAAGGATTTCTAGCACCAAGCTTGGGTCAACTCAGCTTCCTGCAAGAACTGTATCTTCATGGCAACAATCTGATTGGAACAATACCCAAGGAACTGGGGTTACTAAAGAAACTCAAGATCCTTGATTTGGGGATAAATCAACTTTCAGGCCCGATCCCTCCAGAGATAGGGAGTTTAACAGATGTTCTGAAAATG

mRNA sequence

CTGAAAGGATTTCTAGCACCAAGCTTGGGTCAACTCAGCTTCCTGCAAGAACTGTATCTTCATGGCAACAATCTGATTGGAACAATACCCAAGGAACTGGGGTTACTAAAGAAACTCAAGATCCTTGATTTGGGGATAAATCAACTTTCAGGCCCGATCCCTCCAGAGATAGGGAGTTTAACAGATGTTCTGAAAATG

Coding sequence (CDS)

CTGAAAGGATTTCTAGCACCAAGCTTGGGTCAACTCAGCTTCCTGCAAGAACTGTATCTTCATGGCAACAATCTGATTGGAACAATACCCAAGGAACTGGGGTTACTAAAGAAACTCAAGATCCTTGATTTGGGGATAAATCAACTTTCAGGCCCGATCCCTCCAGAGATAGGGAGTTTAACAGATGTTCTGAAAATG

Protein sequence

LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSLTDVLKM
Homology
BLAST of MS012775 vs. NCBI nr
Match: XP_022136546.1 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 [Momordica charantia])

HSP 1 Score: 130.2 bits (326), Expect = 6.6e-27
Identity = 64/66 (96.97%), Postives = 66/66 (100.00%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGIN+LSGPIPPEIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINRLSGPIPPEIGSL 141

Query: 61  TDVLKM 67
           TDVLK+
Sbjct: 142 TDVLKI 147

BLAST of MS012775 vs. NCBI nr
Match: KAG7015185.1 (putative LRR receptor-like serine/threonine-protein kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 123.2 bits (308), Expect = 8.1e-25
Identity = 60/66 (90.91%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TDVLK+
Sbjct: 142 TDVLKI 147

BLAST of MS012775 vs. NCBI nr
Match: KAG6577186.1 (putative LRR receptor-like serine/threonine-protein kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 123.2 bits (308), Expect = 8.1e-25
Identity = 60/66 (90.91%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TDVLK+
Sbjct: 142 TDVLKI 147

BLAST of MS012775 vs. NCBI nr
Match: XP_022931502.1 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 [Cucurbita moschata])

HSP 1 Score: 122.9 bits (307), Expect = 1.1e-24
Identity = 59/66 (89.39%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TD+LK+
Sbjct: 142 TDILKI 147

BLAST of MS012775 vs. NCBI nr
Match: XP_022985455.1 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 [Cucurbita maxima])

HSP 1 Score: 122.9 bits (307), Expect = 1.1e-24
Identity = 59/66 (89.39%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TD+LK+
Sbjct: 142 TDILKI 147

BLAST of MS012775 vs. ExPASy Swiss-Prot
Match: C0LGH8 (Probable LRR receptor-like serine/threonine-protein kinase At1g63430 OS=Arabidopsis thaliana OX=3702 GN=At1g63430 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 8.2e-20
Identity = 46/64 (71.88%), Postives = 54/64 (84.38%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           +KGFLAP LGQ+++LQEL LHGN LIGTIPKE+G LK LKILDLG N L GPIP EIGSL
Sbjct: 81  IKGFLAPELGQITYLQELILHGNILIGTIPKEIGNLKNLKILDLGNNHLMGPIPAEIGSL 140

Query: 61  TDVL 65
           + ++
Sbjct: 141 SGIM 144

BLAST of MS012775 vs. ExPASy Swiss-Prot
Match: Q42371 (LRR receptor-like serine/threonine-protein kinase ERECTA OS=Arabidopsis thaliana OX=3702 GN=ERECTA PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 7.4e-13
Identity = 34/66 (51.52%), Postives = 45/66 (68.18%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           L G + P LG L+F ++LYLH N L G+IP ELG + KL  L+L  N L+G IPPE+G L
Sbjct: 295 LSGSIPPILGNLTFTEKLYLHSNKLTGSIPPELGNMSKLHYLELNDNHLTGHIPPELGKL 354

Query: 61  TDVLKM 67
           TD+  +
Sbjct: 355 TDLFDL 360

BLAST of MS012775 vs. ExPASy Swiss-Prot
Match: Q6XAT2 (LRR receptor-like serine/threonine-protein kinase ERL2 OS=Arabidopsis thaliana OX=3702 GN=ERL2 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.1e-11
Identity = 33/66 (50.00%), Postives = 42/66 (63.64%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           L G + P LG LSF  +LYLHGN L G IP ELG + +L  L L  N+L G IPPE+G L
Sbjct: 300 LTGPIPPILGNLSFTGKLYLHGNKLTGQIPPELGNMSRLSYLQLNDNELVGKIPPELGKL 359

Query: 61  TDVLKM 67
             + ++
Sbjct: 360 EQLFEL 365

BLAST of MS012775 vs. ExPASy Swiss-Prot
Match: B8ABC2 (Leucine-rich repeat protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=LRR1 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.3e-11
Identity = 35/64 (54.69%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           L G L P LG+L  LQ L L+ NN+ GTIP ELG LK L  LDL  N +SG IPP +G L
Sbjct: 78  LSGHLVPELGKLDHLQYLELYKNNIQGTIPSELGNLKNLISLDLYKNNISGTIPPTLGKL 137

Query: 61  TDVL 65
           T ++
Sbjct: 138 TSLV 141

BLAST of MS012775 vs. ExPASy Swiss-Prot
Match: Q5VQP7 (Leucine-rich repeat protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=LRR1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 5.3e-11
Identity = 35/64 (54.69%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           L G L P LG+L  LQ L L+ NN+ GTIP ELG LK L  LDL  N +SG IPP +G L
Sbjct: 78  LSGHLVPELGKLDHLQYLELYKNNIQGTIPSELGNLKNLISLDLYKNNISGTIPPTLGKL 137

Query: 61  TDVL 65
           T ++
Sbjct: 138 TSLV 141

BLAST of MS012775 vs. ExPASy TrEMBL
Match: A0A6J1C3T1 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 OS=Momordica charantia OX=3673 GN=LOC111008220 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.2e-27
Identity = 64/66 (96.97%), Postives = 66/66 (100.00%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGIN+LSGPIPPEIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINRLSGPIPPEIGSL 141

Query: 61  TDVLKM 67
           TDVLK+
Sbjct: 142 TDVLKI 147

BLAST of MS012775 vs. ExPASy TrEMBL
Match: A0A6J1JDB5 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483460 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.1e-25
Identity = 59/66 (89.39%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TD+LK+
Sbjct: 142 TDILKI 147

BLAST of MS012775 vs. ExPASy TrEMBL
Match: A0A6J1JBD1 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483460 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.1e-25
Identity = 59/66 (89.39%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TD+LK+
Sbjct: 142 TDILKI 147

BLAST of MS012775 vs. ExPASy TrEMBL
Match: A0A6J1ETU8 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437658 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.1e-25
Identity = 59/66 (89.39%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TD+LK+
Sbjct: 142 TDILKI 147

BLAST of MS012775 vs. ExPASy TrEMBL
Match: A0A6J1ETT5 (probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437658 PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.1e-25
Identity = 59/66 (89.39%), Postives = 64/66 (96.97%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           LKGFLAPSLGQLSFLQELYLHGNNL+GTIPKELGLLKKLK+LDLG+NQLSG IP EIGSL
Sbjct: 82  LKGFLAPSLGQLSFLQELYLHGNNLLGTIPKELGLLKKLKVLDLGMNQLSGSIPSEIGSL 141

Query: 61  TDVLKM 67
           TD+LK+
Sbjct: 142 TDILKI 147

BLAST of MS012775 vs. TAIR 10
Match: AT1G63430.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 97.1 bits (240), Expect = 5.8e-21
Identity = 46/64 (71.88%), Postives = 54/64 (84.38%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           +KGFLAP LGQ+++LQEL LHGN LIGTIPKE+G LK LKILDLG N L GPIP EIGSL
Sbjct: 81  IKGFLAPELGQITYLQELILHGNILIGTIPKEIGNLKNLKILDLGNNHLMGPIPAEIGSL 140

Query: 61  TDVL 65
           + ++
Sbjct: 141 SGIM 144

BLAST of MS012775 vs. TAIR 10
Match: AT1G63430.2 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 97.1 bits (240), Expect = 5.8e-21
Identity = 46/64 (71.88%), Postives = 54/64 (84.38%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           +KGFLAP LGQ+++LQEL LHGN LIGTIPKE+G LK LKILDLG N L GPIP EIGSL
Sbjct: 81  IKGFLAPELGQITYLQELILHGNILIGTIPKEIGNLKNLKILDLGNNHLMGPIPAEIGSL 140

Query: 61  TDVL 65
           + ++
Sbjct: 141 SGIM 144

BLAST of MS012775 vs. TAIR 10
Match: AT5G41180.1 (leucine-rich repeat transmembrane protein kinase family protein )

HSP 1 Score: 90.5 bits (223), Expect = 5.4e-19
Identity = 42/63 (66.67%), Postives = 52/63 (82.54%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           ++GFL P LGQ+++LQEL L GN L+GTIPKE+G LKKLKILDLG N L+GPIP EIG L
Sbjct: 86  MRGFLVPELGQITYLQELILRGNILMGTIPKEIGKLKKLKILDLGNNHLTGPIPAEIGKL 145

Query: 61  TDV 64
           + +
Sbjct: 146 SRI 148

BLAST of MS012775 vs. TAIR 10
Match: AT2G26330.1 (Leucine-rich receptor-like protein kinase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 5.3e-14
Identity = 34/66 (51.52%), Postives = 45/66 (68.18%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           L G + P LG L+F ++LYLH N L G+IP ELG + KL  L+L  N L+G IPPE+G L
Sbjct: 295 LSGSIPPILGNLTFTEKLYLHSNKLTGSIPPELGNMSKLHYLELNDNHLTGHIPPELGKL 354

Query: 61  TDVLKM 67
           TD+  +
Sbjct: 355 TDLFDL 360

BLAST of MS012775 vs. TAIR 10
Match: AT5G07180.1 (ERECTA-like 2 )

HSP 1 Score: 68.6 bits (166), Expect = 2.2e-12
Identity = 33/66 (50.00%), Postives = 42/66 (63.64%), Query Frame = 0

Query: 1   LKGFLAPSLGQLSFLQELYLHGNNLIGTIPKELGLLKKLKILDLGINQLSGPIPPEIGSL 60
           L G + P LG LSF  +LYLHGN L G IP ELG + +L  L L  N+L G IPPE+G L
Sbjct: 300 LTGPIPPILGNLSFTGKLYLHGNKLTGQIPPELGNMSRLSYLQLNDNELVGKIPPELGKL 359

Query: 61  TDVLKM 67
             + ++
Sbjct: 360 EQLFEL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136546.16.6e-2796.97probable LRR receptor-like serine/threonine-protein kinase At1g63430 [Momordica ... [more]
KAG7015185.18.1e-2590.91putative LRR receptor-like serine/threonine-protein kinase [Cucurbita argyrosper... [more]
KAG6577186.18.1e-2590.91putative LRR receptor-like serine/threonine-protein kinase, partial [Cucurbita a... [more]
XP_022931502.11.1e-2489.39probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 ... [more]
XP_022985455.11.1e-2489.39probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 ... [more]
Match NameE-valueIdentityDescription
C0LGH88.2e-2071.88Probable LRR receptor-like serine/threonine-protein kinase At1g63430 OS=Arabidop... [more]
Q423717.4e-1351.52LRR receptor-like serine/threonine-protein kinase ERECTA OS=Arabidopsis thaliana... [more]
Q6XAT23.1e-1150.00LRR receptor-like serine/threonine-protein kinase ERL2 OS=Arabidopsis thaliana O... [more]
B8ABC25.3e-1154.69Leucine-rich repeat protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=LRR1 PE=... [more]
Q5VQP75.3e-1154.69Leucine-rich repeat protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=LRR1 P... [more]
Match NameE-valueIdentityDescription
A0A6J1C3T13.2e-2796.97probable LRR receptor-like serine/threonine-protein kinase At1g63430 OS=Momordic... [more]
A0A6J1JDB55.1e-2589.39probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X1 ... [more]
A0A6J1JBD15.1e-2589.39probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 ... [more]
A0A6J1ETU85.1e-2589.39probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X2 ... [more]
A0A6J1ETT55.1e-2589.39probable LRR receptor-like serine/threonine-protein kinase At1g63430 isoform X1 ... [more]
Match NameE-valueIdentityDescription
AT1G63430.15.8e-2171.88Leucine-rich repeat protein kinase family protein [more]
AT1G63430.25.8e-2171.88Leucine-rich repeat protein kinase family protein [more]
AT5G41180.15.4e-1966.67leucine-rich repeat transmembrane protein kinase family protein [more]
AT2G26330.15.3e-1451.52Leucine-rich receptor-like protein kinase family protein [more]
AT5G07180.12.2e-1250.00ERECTA-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 15..33
e-value: 0.42
score: 11.3
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 1..65
e-value: 7.1E-18
score: 66.7
NoneNo IPR availablePANTHERPTHR46084:SF19OS04G0534200 PROTEINcoord: 1..66
NoneNo IPR availablePANTHERPTHR46084PROTEIN MALE DISCOVERER 2coord: 1..66
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 1..61

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012775.1MS012775.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding