MS012769 (gene) Bitter gourd (TR) v1

Overview
NameMS012769
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
Locationscaffold63: 3309483 .. 3313618 (-)
RNA-Seq ExpressionMS012769
SyntenyMS012769
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGATTTTTGGATGGAAAGGACCATCTGGGTTCTCAGCCCGTTCTACAGCGGAAGAAGTCACAGAAGGGATTGATGGGAATGGCCTCACTGCTATTGTTACAGGTTTTGTTTCATCTTTTCCCTTTTCCTGTTTGTTCCCCTCTATTCAAATCATTCCTTCGATCGGGGTTTTGTTATTAGTTATTTCCCCTCTTTGCTGGTCACTCTCAGAAGTCGATGGTGATTTCTCTTTTAATCTTTCAGTGTCTCCTTAGTAAATTTGATGCGTTCCACCAATCCGATGTCTAGATGTGGATGTTTAACTTGCTGTTAGATCACTGGCTCACGACTTGATTGCTATTTGTTTTTGTTCTAATGCAAATTTCCGTGCCATTTTTAGTCGTAACATAATACCTTCTAACACTTTCTTGGCCATAATATGAATACTAAGTTTCTTGAGTGTGTTCTGTCGCTATTCCTTGACTGTTCAGTTTCAATCAGGTGTTGGTGGCCTTCATCCTTTTTAATGTGATCTTCTTTTATATGTACTGTGTAGAGATTTATGGAGTAATGCAATTTTCTTTTTGAAGATTTATGTACTGATTGAAATTTAATTGTTAGGTTCATACTTAGCATAATGTTTGTAAGTCTAGAAACTGAGTCAGAATAAGGATCTTTGAATGGCTATTTGCAAGAATGCCTGAAGATTTGATGCTCTGTGCAAGCATCAGGCAAAACATTAGCCGGTTTGGATCGATTTCTAAAAACTGTTTCTGAACATGGACTTTGAAACTTGTTTTCTGTTACTTTTTGCTTGAAAAGAGATTCATCTGGATAGTGTATTTGTTTCCATTTATTTTTAATTTCTATACATGTCACATTAGAAAACATTCTTAACTATATATGAACAATCTTTTAAATCGTTCCAAAGAAATAATATGTAAATGACAAAACTGTGTTCTTGAATGTACAAGGTTTTTTTTTTTCTCGCTACTGTAGTCGGTTAGCCAAAGGTCAATGTTGTCTGGGGAAGTTTTACATTTTTGGATCCTTCTTCTGTCCTTGACCATAATTTTTACTTAAAAAAGGGTCAGCTTTCCTGATCTCATATTGAATCTCTAGATCTAATTCTCACGTTTCTCCTTTTACTATATATTTTACTAGTTTCCCCTCTGATCATTCACTCAAGTTTCTAGCATCATTTTGATCTATAGAAGAACTTTCTCATTTATCTTATTTTGGTTAAAGAATAGTTGAAGTTTTTCTGCTTTTGAAATATCTGGTTCTTTTTTCTTTTCTTACTCATGGTTTCAGGAGCTTCAAGTGGTCTTGGCGAAGAGACCACACGCGTTCTTGCATTACGGGGAGTCTATGTCATTATGGCTGTAAGGAATGTTGAAGCAGGGAAAAAAGTAAAAGAAGCAGTATGCCAGGAATTCCCGTCTGCTAAAATCGATATCATGGAGTTAGATCTTAGTTCAATGGAATCAGTAAGGAAATTCGCATCAGATTATATTTCCTCAGGCCGTGCACTGAATATTCTCATGTAAGAACTGAATTTGGATTCGCTGGACTCAAACTCTTTGCTTTGCCCAGGGGATCTGCCTTTCCACAAGCCTGCATTTCATTAATGCTTTCCTTTTAGTTGAATTTTATAGGATGCCTTGTGCAGGTTTTACAACTCTGAGAATGGATGTTATGCTGTTTATATTTCATTATTTATCTTGACATGTTTTCTTAAGAAATTGGCTGAAATGTTAACAATTGCCTCCAAAAGAAGAGAGTTTTGGATCAAATTCAGCTGAATAGAGAATGATCATTTAACATATATTTTCTTTGTTTTAAGGAACAATGCAGGTGTGATGGCTACTCCTTTTATGCTTTCCCATGATGGCATAGAATTGCAGTTTGCAACGAACCATTTAGGTATGTTTCAAATAAATAATTTTGTCATAAGTACAATTATTTCATTAAGTTTGTTGCTTAAATATAGTCGTTTTTAAGCTTTACGATCCTTTCATGCTTTCCCATGATAAACTTCTAACATGAAATGATCAAAATCTATGAACTATTCAAATATATTTAGAATATTATAATATAATATAAATACAAAGACGTCTTTGACGGGTCCTCGTCGTTCTTGAGGCAGTGATGTGTCATTGTGTTTGAGTTGTATAATGCCCTTGTACGATGTTCATGTTCATGTTTCTTAGATTTTAAAATATCCTCGTCAATGAAGTGCATTTTCTTAGTTAAGCAGAAAGGGTTGTTGATCGATATCTAATTGAACACCATCTTTTCTGATTGGTTGAATAATTGTTTATAATATATCCTTGTATTACTGACCTCTTGGGTTTTATAGATCTACTTCATTACATTATAAAGTTACTCTAAAAGAAAAAAGTTGTCAAACACAAATTTATTGCACTACATCTGATCCATCTATGTTATTCTTAAACATATTCTCTTTCAGATATTCTGACTAGTTACTTTGGATTATTACTCTATTGTAGGACATTTTCTTCTGACAAATCTTCTACTGGATACCATGAAAAAAACTGTGCTTGAAAGCAAAAAAGAGGGAAGGATTGTCAATCTGGCATCTGAGGGTCACCGAGTCACTTATGGCGAAGGAATTCGTTTCGATAAAATCAATGATGAATCAGAGTAACAATCTTAGTTGCTTATTTTGATGATTGAAGCCTCAAAAGTTTCTTTGAATGTGATATAGTAATTCCTTTTGTCATGTGAAATTTCAGGTACAGAACTATCCTAGCTTATGGACAATCAAAGCTTTCCAACATATTGCATGCCAAAGAGCTCGCCAGGCGGTTAAAGGTGCTTATTGGTTTATTCATCAGATTTATAACCAGAGCTTTAAAACTTGTATCTTAAACTAGGCAGAAATTTTCGGTGTAGGATAAGTGACTTGAAATTTCTATCCTTTCAAAATCAGTCATCTTTATTGCTTTTAGAATCTAAAACTTTGTCTTCAACTGTATAAATGTTCAACCGGTCACTCTCTGAAGTGAACTAGGTGGAATTCCCATATCATTCACACGAATTTATCTAAATATATGTTCAATATACATATATTTGATCATCATGACATTATTCCTTGATCATGTAACAGCCTTTTCTGAATAATATATTATCGAATGACTCCAGTAAACTATTAAATAACAGACGGCATAATCATTGGAGATGATTTGTTCTACAGTTCTTTAAACAAATTTATATATTTGATATAGTGTGTGATGGAGCTTGCCTTATTGTGATTTCCCCAGTTATCTTATCAAGTCAAATTTAATTTACGACTATCATTTTTATGTTTTTTGACAGGAAGAAGGGGTAGAAATAACAGCCAACTCCCTTCATCCTGGAGCAATTATTACCAACTTACTACGCTTCCATGGTTTAATTAATGGTAACTTACTCGGCCCTTTGCAAATATAAGGCGAATGTACTTTTGTAGATGCTAAACTGCATGTCTCAGATGCTTGTTTCGATAGTAGAATATACGCGTCTTAATTTTTATCTATTCCAAAAAGGGTGCTAGCCTCCCGCCGAGGTCAAGGATCTCCTCGATATCTTTTCCGAAAAAAAGGTGAAAAAGATTCAAAGGCTCACGTGATTTATAATTCTCCAAGTATTTTTATAAAGGCATCTTTTTCAATCAATTCAGAAGATTTGGTGTTTCAATCAAATTGACCGTTTTAGGTACTCATGAATCACAGATGGAGAACTTCCTATTTTTGAATTAGTGTAGGCTTATATAATGTTTCTTTACCATAAGTTGTCTGTCATCTACTGTTCTGTGTGTATGACTTATCGCTTTTATTACAGCTGTTGCCGGTTCAGTCGCTAAATTTGTGCTTAAAAATGTCCAGCAGGTATGTTAATCATTGATTTCATCAAATTTTCTTGAAGACTATATATTTAGTCTCTCTCAGGTGGGTTTTTATGAGCATAAATTTACATTTGACTGTAGGGAGCTGCAACTCAGTGTTATGTGGCGTTGCATCCCCAAGTCAAGGGGGTAAGTGGAGAGTATTTTGTTGACAGTAATATATCCAACCCAACCAATCACGCCAAGGATAAGGAATTGGCCAAGAAACTGTGGGATTTCAGTGTGGAACTGACCAGCCTTAAA

mRNA sequence

ATGTGGATTTTTGGATGGAAAGGACCATCTGGGTTCTCAGCCCGTTCTACAGCGGAAGAAGTCACAGAAGGGATTGATGGGAATGGCCTCACTGCTATTGTTACAGGAGCTTCAAGTGGTCTTGGCGAAGAGACCACACGCGTTCTTGCATTACGGGGAGTCTATGTCATTATGGCTGTAAGGAATGTTGAAGCAGGGAAAAAAGTAAAAGAAGCAGTATGCCAGGAATTCCCGTCTGCTAAAATCGATATCATGGAGTTAGATCTTAGTTCAATGGAATCAGTAAGGAAATTCGCATCAGATTATATTTCCTCAGGCCGTGCACTGAATATTCTCATGAACAATGCAGGTGTGATGGCTACTCCTTTTATGCTTTCCCATGATGGCATAGAATTGCAGTTTGCAACGAACCATTTAGGACATTTTCTTCTGACAAATCTTCTACTGGATACCATGAAAAAAACTGTGCTTGAAAGCAAAAAAGAGGGAAGGATTGTCAATCTGGCATCTGAGGGTCACCGAGTCACTTATGGCGAAGGAATTCGTTTCGATAAAATCAATGATGAATCAGAGTACAGAACTATCCTAGCTTATGGACAATCAAAGCTTTCCAACATATTGCATGCCAAAGAGCTCGCCAGGCGGTTAAAGGAAGAAGGGGTAGAAATAACAGCCAACTCCCTTCATCCTGGAGCAATTATTACCAACTTACTACGCTTCCATGGTTTAATTAATGCTGTTGCCGGTTCAGTCGCTAAATTTGTGCTTAAAAATGTCCAGCAGGGAGCTGCAACTCAGTGTTATGTGGCGTTGCATCCCCAAGTCAAGGGGGTAAGTGGAGAGTATTTTGTTGACAGTAATATATCCAACCCAACCAATCACGCCAAGGATAAGGAATTGGCCAAGAAACTGTGGGATTTCAGTGTGGAACTGACCAGCCTTAAA

Coding sequence (CDS)

ATGTGGATTTTTGGATGGAAAGGACCATCTGGGTTCTCAGCCCGTTCTACAGCGGAAGAAGTCACAGAAGGGATTGATGGGAATGGCCTCACTGCTATTGTTACAGGAGCTTCAAGTGGTCTTGGCGAAGAGACCACACGCGTTCTTGCATTACGGGGAGTCTATGTCATTATGGCTGTAAGGAATGTTGAAGCAGGGAAAAAAGTAAAAGAAGCAGTATGCCAGGAATTCCCGTCTGCTAAAATCGATATCATGGAGTTAGATCTTAGTTCAATGGAATCAGTAAGGAAATTCGCATCAGATTATATTTCCTCAGGCCGTGCACTGAATATTCTCATGAACAATGCAGGTGTGATGGCTACTCCTTTTATGCTTTCCCATGATGGCATAGAATTGCAGTTTGCAACGAACCATTTAGGACATTTTCTTCTGACAAATCTTCTACTGGATACCATGAAAAAAACTGTGCTTGAAAGCAAAAAAGAGGGAAGGATTGTCAATCTGGCATCTGAGGGTCACCGAGTCACTTATGGCGAAGGAATTCGTTTCGATAAAATCAATGATGAATCAGAGTACAGAACTATCCTAGCTTATGGACAATCAAAGCTTTCCAACATATTGCATGCCAAAGAGCTCGCCAGGCGGTTAAAGGAAGAAGGGGTAGAAATAACAGCCAACTCCCTTCATCCTGGAGCAATTATTACCAACTTACTACGCTTCCATGGTTTAATTAATGCTGTTGCCGGTTCAGTCGCTAAATTTGTGCTTAAAAATGTCCAGCAGGGAGCTGCAACTCAGTGTTATGTGGCGTTGCATCCCCAAGTCAAGGGGGTAAGTGGAGAGTATTTTGTTGACAGTAATATATCCAACCCAACCAATCACGCCAAGGATAAGGAATTGGCCAAGAAACTGTGGGATTTCAGTGTGGAACTGACCAGCCTTAAA

Protein sequence

MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAVRNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMATPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEGIRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRFHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKELAKKLWDFSVELTSLK
Homology
[Close]
BLAST of MS012769 vs. NCBI nr
Match: XP_022136624.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Momordica charantia])

HSP 1 Score: 610.9 bits (1574), Expect = 6.1e-171
Identity = 313/315 (99.37%), Postives = 313/315 (99.37%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG
Sbjct: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF
Sbjct: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240

Query: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKEL 300
           HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYF DSNISNPTNHAKDKEL
Sbjct: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFADSNISNPTNHAKDKEL 300

Query: 301 AKKLWDFSVELTSLK 316
           AKKLWDFSVELTS K
Sbjct: 301 AKKLWDFSVELTSPK 315

[Close]
BLAST of MS012769 vs. NCBI nr
Match: XP_038884091.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 575.5 bits (1482), Expect = 2.9e-160
Identity = 293/315 (93.02%), Postives = 305/315 (96.83%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAG+KVKEAV +E PSAKIDIMELDLSSMESVRKFA+DYI+SGRALNILMNNAGVMA
Sbjct: 61  RNVEAGRKVKEAVLKESPSAKIDIMELDLSSMESVRKFAADYIASGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDGIELQFATNHLGHFLLTNLL++TMKKTVLESKKEGRIVNL+SEGHR+TY EG
Sbjct: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLMETMKKTVLESKKEGRIVNLSSEGHRLTYSEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLK EGVEITAN+LHPGAI TNLLRF
Sbjct: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKAEGVEITANALHPGAIATNLLRF 240

Query: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKEL 300
           HG INAV  SVAKFVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPTNHAKD EL
Sbjct: 241 HGFINAVTNSVAKFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTNHAKDMEL 300

Query: 301 AKKLWDFSVELTSLK 316
           AKKLWDFSV+LTS K
Sbjct: 301 AKKLWDFSVDLTSPK 315

[Close]
BLAST of MS012769 vs. NCBI nr
Match: XP_023552364.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552365.1 short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569.3 bits (1466), Expect = 2.0e-158
Identity = 290/316 (91.77%), Postives = 306/316 (96.84%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGW GPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWTGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAV +E PSAKID+MELDLSSMESVRKFASDYI+SGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVLKESPSAKIDVMELDLSSMESVRKFASDYIASGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDGIELQFATNH+GHFLLTNLLL+TMKKTV+ESKKEGRIVNL+SEGHR+TYGEG
Sbjct: 121 TPFMLSHDGIELQFATNHIGHFLLTNLLLETMKKTVVESKKEGRIVNLSSEGHRLTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAI-ITNLLR 240
           +RFDKINDESEYRTI AYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI  TNLLR
Sbjct: 181 VRFDKINDESEYRTIFAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIAATNLLR 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           FHGLINAVA S+A FVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPTNHAKD E
Sbjct: 241 FHGLINAVATSIATFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTNHAKDME 300

Query: 301 LAKKLWDFSVELTSLK 316
           LAKKLWDFS++LT+ K
Sbjct: 301 LAKKLWDFSMDLTTPK 316

[Close]
BLAST of MS012769 vs. NCBI nr
Match: XP_022984715.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 568.2 bits (1463), Expect = 4.6e-158
Identity = 290/316 (91.77%), Postives = 306/316 (96.84%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGW GPSGFSARSTA+EVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWTGPSGFSARSTAKEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAV +E PSAKID+MELDLSSMESVRKFASDYI+SGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVLKESPSAKIDVMELDLSSMESVRKFASDYIASGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDGIELQFATNH+GHFLLTNLLL+TMKKTV+ESKKEGRIVNL+SEGHR+TYGEG
Sbjct: 121 TPFMLSHDGIELQFATNHIGHFLLTNLLLETMKKTVVESKKEGRIVNLSSEGHRLTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAI-ITNLLR 240
           +RFDKINDESEYRTI AYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI  TNLLR
Sbjct: 181 VRFDKINDESEYRTIFAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIAATNLLR 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           FHGLINAVA SVA FVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPTNHAKD E
Sbjct: 241 FHGLINAVATSVATFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTNHAKDME 300

Query: 301 LAKKLWDFSVELTSLK 316
           LAKKLWDFS++LT+ K
Sbjct: 301 LAKKLWDFSMDLTTPK 316

[Close]
BLAST of MS012769 vs. NCBI nr
Match: KAG6577191.1 (Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.0 bits (1460), Expect = 1.0e-157
Identity = 288/316 (91.14%), Postives = 306/316 (96.84%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGW GPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWTGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAV ++ PSAKID+MELDLSSMESVRKFASDYI+SGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVLKQSPSAKIDVMELDLSSMESVRKFASDYIASGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDG+ELQFATNH+GHFLLTNLLL+TMKKTV+ESKKEGRIVNL+SEGHR+TYGEG
Sbjct: 121 TPFMLSHDGMELQFATNHIGHFLLTNLLLETMKKTVVESKKEGRIVNLSSEGHRLTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAI-ITNLLR 240
           +RFDKINDESEYRTI AYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI  TNLLR
Sbjct: 181 VRFDKINDESEYRTIFAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIAATNLLR 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           FHGLINAVA S+A FVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPTNHAKD E
Sbjct: 241 FHGLINAVATSIATFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTNHAKDME 300

Query: 301 LAKKLWDFSVELTSLK 316
           LAKKLWDFS++LT+ K
Sbjct: 301 LAKKLWDFSMDLTTPK 316

[Close]
BLAST of MS012769 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 7.1e-122
Identity = 222/313 (70.93%), Postives = 262/313 (83.71%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MW FG KG SGFS+RSTAEEVT G+DG GLTAIVTGASSG+G ET RVL+LRGV+V+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RN ++G KVKE + ++ P AK+D+MELDLSSM+SVRKFAS+Y S+G  LN+L+NNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
            PFMLS D IELQFATNHLGHFLLT LLLDTMK T  ESK+EGRIVNL+SE HR +Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           +RFDKIND+S Y ++ AYGQSKL N+LHA EL ++LKE+GV ITANSLHPGAI+TNL R+
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 HGLINAVA-GSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
                AVA G+VAK++LK+V QGAAT CYVAL+PQV GVSGEYF DSNI+ P    KD E
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKKLWDFSVELT 313
           LAKK+WDFS +LT
Sbjct: 301 LAKKVWDFSTKLT 313

[Close]
BLAST of MS012769 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.8e-120
Identity = 219/316 (69.30%), Postives = 252/316 (79.75%), Query Frame = 0 

Query: 1   MWIF-GWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMA 60
           MW F   KG SGFS  STAE+VT GID  GLTAIVTGASSG+G ETTRVLALRG +VIM 
Sbjct: 1   MWPFSSKKGVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMG 60

Query: 61  VRNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVM 120
           VRN+ A K VK+ + ++ PSAK+D +ELDLSS++SV+KFAS++ SSGR LNIL+NNAG+M
Sbjct: 61  VRNMVAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIM 120

Query: 121 ATPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGE 180
           A PF LS D IELQFATNH+GHFLLTNLLLDTMKKT  ESKKEGRIVN+ASE HR  Y E
Sbjct: 121 ACPFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPE 180

Query: 181 GIRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLR 240
           GIRFDKIND+S Y    AYGQSKL+N+LHA +L + LKE+GV ITANSLHPG I+TNL R
Sbjct: 181 GIRFDKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
            +  +N +   + K VLKNVQQGAAT CYVALHPQVKGVSGEYF DSN+   T H KD +
Sbjct: 241 HNSAVNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVD 300

Query: 301 LAKKLWDFSVELTSLK 316
           LAKKLWDFS+ L   K
Sbjct: 301 LAKKLWDFSINLVKQK 316

[Close]
BLAST of MS012769 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 7.4e-95
Identity = 177/309 (57.28%), Postives = 223/309 (72.17%), Query Frame = 0 

Query: 3   IFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAVRN 62
           + G  G SG+ + STAE+VT  ID   LTAI+TG +SG+G E  RVL +RG +VI+A RN
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAARN 66

Query: 63  VEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMATP 122
            +A    KE + Q +P+A+ID ++LDLSS++SVR F   +++    LNIL+NNAGVM  P
Sbjct: 67  TKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCP 126

Query: 123 FMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEGIR 182
           F LS DGIE QFATNH+GHFLLTNLLLD MK +  ES  EGRIVNL+S  H  TY EGI 
Sbjct: 127 FQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIM 186

Query: 183 FDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRFHG 242
           FD IND   Y    AYGQSKL+N+LH+  L+R+L+EEGV IT NS+HPG I TNL R  G
Sbjct: 187 FDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHSG 246

Query: 243 LINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKELAK 302
           L  AV  +++ F+ KN+ QGAAT CYVALHP +KGV+G+YF D N++ P+N A D  LA 
Sbjct: 247 LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTLAD 306

Query: 303 KLWDFSVEL 312
           KLWDFS++L
Sbjct: 307 KLWDFSIKL 315

[Close]
BLAST of MS012769 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.2e-94
Identity = 176/309 (56.96%), Postives = 222/309 (71.84%), Query Frame = 0 

Query: 3   IFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAVRN 62
           + G  G SG+ + STAE+VT  ID   LTAI+TG +SG+G E  RVL +RG +VI+A RN
Sbjct: 7   VTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASRN 66

Query: 63  VEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMATP 122
            +A    KE + Q +P+A+ID ++LDLSS++SVR F   +++    LNIL+NNAGVM  P
Sbjct: 67  TKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCP 126

Query: 123 FMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEGIR 182
           F LS DGIE QFATNH+GHFLLTNLLLD MK +  ES  EGRIVNL+S  H  TY EGI 
Sbjct: 127 FQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIM 186

Query: 183 FDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRFHG 242
           FD IND   Y    AYGQSKL+N+LH+  L+R+L+EEGV IT NS+HPG I TNL R  G
Sbjct: 187 FDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHSG 246

Query: 243 LINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKELAK 302
           L  AV  +++ F+ KN+ QGAAT CYVALHP +K V+G+YF D N++ P+N A D  LA 
Sbjct: 247 LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTLAD 306

Query: 303 KLWDFSVEL 312
           KLWDFS++L
Sbjct: 307 KLWDFSIKL 315

[Close]
BLAST of MS012769 vs. ExPASy Swiss-Prot
Match: Q5F389 (WW domain-containing oxidoreductase OS=Gallus gallus OX=9031 GN=WWOX PE=2 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-47
Identity = 114/305 (37.38%), Postives = 175/305 (57.38%), Query Frame = 0 

Query: 12  FSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAVRNVEAGKKVKE 71
           +   STA E+ +G D +G   I+TGA+SG+G ET +  AL G YVI+A RN+  G    +
Sbjct: 107 YDGNSTAMEILQGRDLSGKVIIITGANSGIGFETAKSFALHGAYVILACRNMSRGNDAVQ 166

Query: 72  AVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMATPFMLSHDGIE 131
            + +E+  AK++ M LDL+S+ SV+ FA  + S    L+IL+ NA +  + + L+ DG+E
Sbjct: 167 RILEEWHKAKVEAMTLDLASLRSVQNFAEAFKSKNMPLHILVCNAAIFGSSWCLTEDGLE 226

Query: 132 LQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVT----YGEGIRFDKIN 191
             F  NHLGHF L  LL D     +L      R+V ++SE HR T        + F  ++
Sbjct: 227 STFQVNHLGHFYLVQLLED-----ILRRSSPARVVVVSSESHRFTEIKDSSGKLDFSLLS 286

Query: 192 -DESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRFHGLINA 251
             + EY  +LAY +SKL NIL + EL RRL   GV  T+NS+HPG +I + +  +  +  
Sbjct: 287 PSKKEYWAMLAYNRSKLCNILFSNELNRRLSPHGV--TSNSVHPGNMIYSSIHRNWWVYT 346

Query: 252 VAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKELAKKLWD 311
           +  ++A+   K++QQGAAT  Y A   +++G+ G YF +     P+  A+++  A  LW+
Sbjct: 347 LLFTLARPFTKSMQQGAATTVYCATAAELEGLGGMYFNNCCRCLPSAEARNELTAVALWE 404

[Close]
BLAST of MS012769 vs. ExPASy TrEMBL
Match: A0A6J1C4U8 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008286 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 313/315 (99.37%), Postives = 313/315 (99.37%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG
Sbjct: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF
Sbjct: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240

Query: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKEL 300
           HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYF DSNISNPTNHAKDKEL
Sbjct: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFADSNISNPTNHAKDKEL 300

Query: 301 AKKLWDFSVELTSLK 316
           AKKLWDFSVELTS K
Sbjct: 301 AKKLWDFSVELTSPK 315

[Close]
BLAST of MS012769 vs. ExPASy TrEMBL
Match: A0A6J1J9D0 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482915 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.2e-158
Identity = 290/316 (91.77%), Postives = 306/316 (96.84%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGW GPSGFSARSTA+EVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWTGPSGFSARSTAKEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAV +E PSAKID+MELDLSSMESVRKFASDYI+SGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVLKESPSAKIDVMELDLSSMESVRKFASDYIASGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDGIELQFATNH+GHFLLTNLLL+TMKKTV+ESKKEGRIVNL+SEGHR+TYGEG
Sbjct: 121 TPFMLSHDGIELQFATNHIGHFLLTNLLLETMKKTVVESKKEGRIVNLSSEGHRLTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAI-ITNLLR 240
           +RFDKINDESEYRTI AYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI  TNLLR
Sbjct: 181 VRFDKINDESEYRTIFAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIAATNLLR 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           FHGLINAVA SVA FVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPTNHAKD E
Sbjct: 241 FHGLINAVATSVATFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTNHAKDME 300

Query: 301 LAKKLWDFSVELTSLK 316
           LAKKLWDFS++LT+ K
Sbjct: 301 LAKKLWDFSMDLTTPK 316

[Close]
BLAST of MS012769 vs. ExPASy TrEMBL
Match: A0A6J1EYV4 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111437662 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.4e-157
Identity = 287/316 (90.82%), Postives = 305/316 (96.52%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGW GPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV
Sbjct: 1   MWIFGWTGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAGKKVKEAV +E PSAKID+MELDLSSMESVRKFASDYI+SGRALNILMNNAGVMA
Sbjct: 61  RNVEAGKKVKEAVLKESPSAKIDVMELDLSSMESVRKFASDYIASGRALNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPFMLSHDG+ELQFATNH+GHFLLTNLLL+TMKKTV+E KKEGRIVNL+SEGHR+TYGEG
Sbjct: 121 TPFMLSHDGMELQFATNHIGHFLLTNLLLETMKKTVVECKKEGRIVNLSSEGHRLTYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAI-ITNLLR 240
           +RFDKINDESEYRTI AYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI  TNLLR
Sbjct: 181 VRFDKINDESEYRTIFAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIAATNLLR 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           FHGLINAVA S+A FVLKNV+QGAATQCYVAL+PQVKGVSGEYFVDSNI+NPTNHAKD E
Sbjct: 241 FHGLINAVATSIATFVLKNVRQGAATQCYVALNPQVKGVSGEYFVDSNIANPTNHAKDME 300

Query: 301 LAKKLWDFSVELTSLK 316
           LAKKLWDFS++LT+ K
Sbjct: 301 LAKKLWDFSMDLTTPK 316

[Close]
BLAST of MS012769 vs. ExPASy TrEMBL
Match: A0A5D3D084 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004620 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.0e-156
Identity = 284/315 (90.16%), Postives = 301/315 (95.56%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEE+TRVLALRGVYVIMAV
Sbjct: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEESTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAG+KVKEAV +E PSAKID+MELDLSSMESVRKFA+DYI+SGR LNILMNNAGVMA
Sbjct: 61  RNVEAGRKVKEAVLKESPSAKIDVMELDLSSMESVRKFAADYIASGRPLNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPF LSHDGIELQFATNHLGHFLLTNLLL+TMKKTVLESKKEGRIVNL+SEGHR+ YGEG
Sbjct: 121 TPFTLSHDGIELQFATNHLGHFLLTNLLLETMKKTVLESKKEGRIVNLSSEGHRMAYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           IRFDKIN+E+EYRTILAYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI TNLLRF
Sbjct: 181 IRFDKINNEAEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIATNLLRF 240

Query: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKEL 300
           H  INAV   VAKFVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPT HAKD +L
Sbjct: 241 HSTINAVTNLVAKFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTKHAKDMDL 300

Query: 301 AKKLWDFSVELTSLK 316
           AKKLWDFSV+LT+ K
Sbjct: 301 AKKLWDFSVDLTNPK 315

[Close]
BLAST of MS012769 vs. ExPASy TrEMBL
Match: A0A1S3BU18 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103493161 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.0e-156
Identity = 284/315 (90.16%), Postives = 301/315 (95.56%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEE+TRVLALRGVYVIMAV
Sbjct: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEESTRVLALRGVYVIMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RNVEAG+KVKEAV +E PSAKID+MELDLSSMESVRKFA+DYI+SGR LNILMNNAGVMA
Sbjct: 61  RNVEAGRKVKEAVLKESPSAKIDVMELDLSSMESVRKFAADYIASGRPLNILMNNAGVMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
           TPF LSHDGIELQFATNHLGHFLLTNLLL+TMKKTVLESKKEGRIVNL+SEGHR+ YGEG
Sbjct: 121 TPFTLSHDGIELQFATNHLGHFLLTNLLLETMKKTVLESKKEGRIVNLSSEGHRMAYGEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           IRFDKIN+E+EYRTILAYGQSKLSNILHAKELARRLKEEGVEITAN+LHPGAI TNLLRF
Sbjct: 181 IRFDKINNEAEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANALHPGAIATNLLRF 240

Query: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKEL 300
           H  INAV   VAKFVLKNVQQGAATQCYVAL+PQVKGVSGEYFVDSNI+NPT HAKD +L
Sbjct: 241 HSTINAVTNLVAKFVLKNVQQGAATQCYVALNPQVKGVSGEYFVDSNIANPTKHAKDMDL 300

Query: 301 AKKLWDFSVELTSLK 316
           AKKLWDFSV+LT+ K
Sbjct: 301 AKKLWDFSVDLTNPK 315

[Close]
BLAST of MS012769 vs. TAIR 10
Match: AT4G11410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 448.4 bits (1152), Expect = 4.9e-126
Identity = 219/312 (70.19%), Postives = 260/312 (83.33%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MW F WKG SGFSARSTAEEVT GIDG GLTAIVTGASSG+GEETTRVLALRGV+V+MAV
Sbjct: 1   MWPFWWKGASGFSARSTAEEVTHGIDGTGLTAIVTGASSGIGEETTRVLALRGVHVVMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RN ++G +V++ + +E P AKID+M+LDLSSM SVR FAS+Y S    LN+L+NNAG+MA
Sbjct: 61  RNTDSGNQVRDKILKEIPQAKIDVMKLDLSSMASVRSFASEYQSLDLPLNLLINNAGIMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
            PF+LS D IELQFATNHLGHFLLTNLLL+ MKKT  ES +EGRIV ++SEGHR  Y EG
Sbjct: 121 CPFLLSSDNIELQFATNHLGHFLLTNLLLERMKKTASESNREGRIVIVSSEGHRFAYREG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           ++FDKINDE+ Y T+ AYGQSKL NILHA ELAR  KE+GV ITANSLHPG+I+TNLLR+
Sbjct: 181 VQFDKINDEARYNTLQAYGQSKLGNILHATELARLFKEQGVNITANSLHPGSIMTNLLRY 240

Query: 241 HGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKEL 300
           H  IN +  +V K+VLK++ QGAAT CY ALHPQ KGVSGEY +D+NIS+P +  KDK+L
Sbjct: 241 HSFINTIGNAVGKYVLKSIPQGAATTCYAALHPQAKGVSGEYLMDNNISDPNSQGKDKDL 300

Query: 301 AKKLWDFSVELT 313
           AKKLW+FS+ LT
Sbjct: 301 AKKLWEFSLRLT 312

[Close]
BLAST of MS012769 vs. TAIR 10
Match: AT4G23420.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 3.9e-123
Identity = 222/313 (70.93%), Postives = 260/313 (83.07%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MW FG KG SGFS+RSTAEEVT G+DG GLTAIVTGASSG+G ET RVLALRGV+V+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RN  AG KVKE + ++ P AK+D+MEL+LSSMESVRKFAS+Y S+G  LN+L+NNAG+MA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
            PFMLS D IELQFATNHLGHFLLT LLLDTMK T  ESK+EGRIVN++SE HR +Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLL-R 240
           +RFDKINDES Y +I AYGQSKL N+LHA ELA++LKE+GV ITANSLHPGAI+TNL   
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           F+  +    G+VAK+++K+V QGAAT CYVAL+PQV GV+GEYF DSNI+ P    KD E
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTE 300

Query: 301 LAKKLWDFSVELT 313
           LAKKLWDFS +LT
Sbjct: 301 LAKKLWDFSTKLT 313

[Close]
BLAST of MS012769 vs. TAIR 10
Match: AT4G23420.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 3.9e-123
Identity = 222/313 (70.93%), Postives = 260/313 (83.07%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MW FG KG SGFS+RSTAEEVT G+DG GLTAIVTGASSG+G ET RVLALRGV+V+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RN  AG KVKE + ++ P AK+D+MEL+LSSMESVRKFAS+Y S+G  LN+L+NNAG+MA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
            PFMLS D IELQFATNHLGHFLLT LLLDTMK T  ESK+EGRIVN++SE HR +Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLL-R 240
           +RFDKINDES Y +I AYGQSKL N+LHA ELA++LKE+GV ITANSLHPGAI+TNL   
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 241 FHGLINAVAGSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
           F+  +    G+VAK+++K+V QGAAT CYVAL+PQV GV+GEYF DSNI+ P    KD E
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTE 300

Query: 301 LAKKLWDFSVELT 313
           LAKKLWDFS +LT
Sbjct: 301 LAKKLWDFSTKLT 313

[Close]
BLAST of MS012769 vs. TAIR 10
Match: AT4G23430.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 438.3 bits (1126), Expect = 5.1e-123
Identity = 222/313 (70.93%), Postives = 262/313 (83.71%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MW FG KG SGFS+RSTAEEVT G+DG GLTAIVTGASSG+G ET RVL+LRGV+V+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RN ++G KVKE + ++ P AK+D+MELDLSSM+SVRKFAS+Y S+G  LN+L+NNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
            PFMLS D IELQFATNHLGHFLLT LLLDTMK T  ESK+EGRIVNL+SE HR +Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           +RFDKIND+S Y ++ AYGQSKL N+LHA EL ++LKE+GV ITANSLHPGAI+TNL R+
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 HGLINAVA-GSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
                AVA G+VAK++LK+V QGAAT CYVAL+PQV GVSGEYF DSNI+ P    KD E
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKKLWDFSVELT 313
           LAKK+WDFS +LT
Sbjct: 301 LAKKVWDFSTKLT 313

[Close]
BLAST of MS012769 vs. TAIR 10
Match: AT4G23430.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 431.4 bits (1108), Expect = 6.2e-121
Identity = 221/313 (70.61%), Postives = 261/313 (83.39%), Query Frame = 0 

Query: 1   MWIFGWKGPSGFSARSTAEEVTEGIDGNGLTAIVTGASSGLGEETTRVLALRGVYVIMAV 60
           MW FG KG SGFS+RSTAEEVT G+DG GLTAIVTGASSG+G ET RVL+LRGV+V+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNVEAGKKVKEAVCQEFPSAKIDIMELDLSSMESVRKFASDYISSGRALNILMNNAGVMA 120
           RN ++G KVKE + ++ P AK+D+MELDLSSM+SVRKFAS+Y S+G  LN+L+NNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 TPFMLSHDGIELQFATNHLGHFLLTNLLLDTMKKTVLESKKEGRIVNLASEGHRVTYGEG 180
            PFMLS D IELQFATNHLGHFLLT LLLDTMK T  ESK+EGRIVNL+SE HR +Y EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDKINDESEYRTILAYGQSKLSNILHAKELARRLKEEGVEITANSLHPGAIITNLLRF 240
           +RFDKIND+S   ++ AYGQSKL N+LHA EL ++LKE+GV ITANSLHPGAI+TNL R+
Sbjct: 181 VRFDKINDKSS--SMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 HGLINAVA-GSVAKFVLKNVQQGAATQCYVALHPQVKGVSGEYFVDSNISNPTNHAKDKE 300
                AVA G+VAK++LK+V QGAAT CYVAL+PQV GVSGEYF DSNI+ P    KD E
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKKLWDFSVELT 313
           LAKK+WDFS +LT
Sbjct: 301 LAKKVWDFSTKLT 311

The following BLAST results are available for this feature:
BLAST of MS012769 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022136624.16.1e-17199.37short-chain dehydrogenase TIC 32, chloroplastic-like [Momordica charantia][more]
XP_038884091.12.9e-16093.02short-chain dehydrogenase TIC 32, chloroplastic-like [Benincasa hispida][more]
XP_023552364.12.0e-15891.77short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo ... [more]
XP_022984715.14.6e-15891.77short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima][more]
KAG6577191.11.0e-15791.14Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma... [more]
BLAST of MS012769 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A2RVM07.1e-12270.93Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q6RVV41.8e-12069.30Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
A0A078ISJ67.4e-9557.28Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078IS668.2e-9456.96Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
Q5F3892.0e-4737.38WW domain-containing oxidoreductase OS=Gallus gallus OX=9031 GN=WWOX PE=2 SV=2[more]
BLAST of MS012769 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C4U83.0e-17199.37short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3... [more]
A0A6J1J9D02.2e-15891.77short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1EYV42.4e-15790.82short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36... [more]
A0A5D3D0846.0e-15690.16Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BU186.0e-15690.16short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=... [more]
BLAST of MS012769 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G11410.14.9e-12670.19NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23420.13.9e-12370.93NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23420.23.9e-12370.93NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23430.25.1e-12370.93NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23430.16.2e-12170.61NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 221..238
score: 32.18
coord: 157..173
score: 29.26
coord: 107..118
score: 44.33
coord: 198..217
score: 27.16
coord: 31..48
score: 40.58
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 163..171
score: 51.1
coord: 107..118
score: 42.56
coord: 198..217
score: 25.97
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 31..175
e-value: 2.0E-23
score: 82.9
NoneNo IPR availableGENE3D3.40.50.720coord: 24..315
e-value: 3.6E-75
score: 254.7
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 2..312
NoneNo IPR availablePANTHERPTHR24320:SF230NAD(P)-BINDING ROSSMANN-FOLD PROTEINcoord: 2..312
NoneNo IPR availableCDDcd05327retinol-DH_like_SDR_c_likecoord: 29..305
e-value: 9.52766E-102
score: 297.599
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 29..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012769.1MS012769.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity