MS012766 (gene) Bitter gourd (TR) v1

BLAST of MS012766 vs. NCBI nr
Match: XP_022136960.1 (subtilisin-like protease SBT3.5 isoform X2 [Momordica charantia])

HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 707/716 (98.74%), Postives = 708/716 (98.88%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS
Sbjct: 55  KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 114

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P PYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRG CKSGEKFNASR
Sbjct: 115 PSPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGTCKSGEKFNASR 174

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCNRKIIGARWYVDGFK EFGEFNRS ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG
Sbjct: 175 NCNRKIIGARWYVDGFKAEFGEFNRSSATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 234

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 240
           LAGGTLRGGAPRARLAMYKACWSLP GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP
Sbjct: 235 LAGGTLRGGAPRARLAMYKACWSLPSGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 294

Query: 241 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLA 300
           LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAA SMDRTFLA
Sbjct: 295 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAASSMDRTFLA 354

Query: 301 TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC 360
           TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC
Sbjct: 355 TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC 414

Query: 361 FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY 420
           FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY
Sbjct: 415 FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY 474

Query: 421 IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL 480
           IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL
Sbjct: 475 IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL 534

Query: 481 DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVPIL 540
           DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAI+TTAWNTHSSSGVPIL
Sbjct: 535 DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIVTTAWNTHSSSGVPIL 594

Query: 541 TEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT 600
           TEGSP KRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT
Sbjct: 595 TEGSPWKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT 654

Query: 601 ECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP 660
           ECPTQDQISILDLNLPSITVPALSNW TVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP
Sbjct: 655 ECPTQDQISILDLNLPSITVPALSNWNTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP 714

Query: 661 VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
           VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH
Sbjct: 715 VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 770

BLAST of MS012766 vs. NCBI nr
Match: XP_022136958.1 (subtilisin-like protease SBT3.5 isoform X1 [Momordica charantia])

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 707/726 (97.38%), Postives = 708/726 (97.52%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS
Sbjct: 55  KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 114

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P PYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRG CKSGEKFNASR
Sbjct: 115 PSPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGTCKSGEKFNASR 174

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCNRKIIGARWYVDGFK EFGEFNRS ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG
Sbjct: 175 NCNRKIIGARWYVDGFKAEFGEFNRSSATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 234

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 240
           LAGGTLRGGAPRARLAMYKACWSLP GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP
Sbjct: 235 LAGGTLRGGAPRARLAMYKACWSLPSGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 294

Query: 241 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLA 300
           LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAA SMDRTFLA
Sbjct: 295 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAASSMDRTFLA 354

Query: 301 TIVLGNNNTFL----------GQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGS 360
           TIVLGNNNTFL          GQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGS
Sbjct: 355 TIVLGNNNTFLVGLIWPYLLQGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGS 414

Query: 361 KWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVD 420
           KWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVD
Sbjct: 415 KWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVD 474

Query: 421 LEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPG 480
           LEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPG
Sbjct: 475 LEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPG 534

Query: 481 VAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWN 540
           VAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAI+TTAWN
Sbjct: 535 VAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIVTTAWN 594

Query: 541 THSSSGVPILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNS 600
           THSSSGVPILTEGSP KRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNS
Sbjct: 595 THSSSGVPILTEGSPWKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNS 654

Query: 601 AISVLTQDKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPP 660
           AISVLTQDKTECPTQDQISILDLNLPSITVPALSNW TVTRTVTNVGNLTSVYRVVIDPP
Sbjct: 655 AISVLTQDKTECPTQDQISILDLNLPSITVPALSNWNTVTRTVTNVGNLTSVYRVVIDPP 714

Query: 661 IGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQ 717
           IGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQ
Sbjct: 715 IGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQ 774

BLAST of MS012766 vs. NCBI nr
Match: XP_021679638.1 (subtilisin-like protease SBT3.3 [Hevea brasiliensis])

HSP 1 Score: 885.6 bits (2287), Expect = 2.9e-253
Identity = 440/718 (61.28%), Postives = 558/718 (77.72%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K+ AS+  VYSYKH FSGFAAKLT+SQA +LAE+PGV+RV+PN L+ L TTRSWDFLG+S
Sbjct: 80  KELASQLIVYSYKHGFSGFAAKLTESQAQKLAELPGVVRVIPNSLHKLQTTRSWDFLGLS 139

Query: 61  PFPYSP-NLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNAS 120
              YSP N L  SN+GDG+IIG+ D+GIWPES+AF+D+GLGP+P RW+G+CKSG++FNA+
Sbjct: 140 --SYSPVNALQRSNMGDGVIIGVFDTGIWPESKAFSDEGLGPIPFRWKGVCKSGKQFNAT 199

Query: 121 RNCNRKIIGARWYVDGFKVEFGE-FNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSY 180
            NCN+KIIGARWY+DGF  E+G+  N S   EF+SPRD+NGHGT TASTAAG+F+ NVSY
Sbjct: 200 LNCNKKIIGARWYIDGFLAEYGKPLNTSEDLEFLSPRDANGHGTHTASTAAGAFIRNVSY 259

Query: 181 RGLAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKS 240
           +GL  GT+RGGAPRARLA+YK CW++   GGQC++AD+LKA D+A+ D VDVLSLS+G S
Sbjct: 260 KGLGPGTIRGGAPRARLAIYKVCWNV--FGGQCSSADMLKAFDEAIYDGVDVLSLSIGSS 319

Query: 241 VPLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTF 300
           +PLF ++DE + +A GSFHA++R I VVC A NDGPS++TV NT+PWIL VAA +MDR F
Sbjct: 320 IPLFSDIDERDGIATGSFHAVARGITVVCGAANDGPSAQTVQNTAPWILTVAASTMDRAF 379

Query: 301 LATIVLGNNNTFLGQTLFSGEEIGYSGLVYP-GSGLRQEVAGLCELVPLVNGSKWMAGKV 360
              I LGNN TFLGQ +F+G+EIG+ GLVYP  SGL    AG+C+ + L   +  +AGKV
Sbjct: 380 PTPITLGNNKTFLGQAMFTGKEIGFRGLVYPEASGLDPNAAGVCQSLSL--NATLVAGKV 439

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+        A++ AAE VKA  GVGLIVA++P D  + C+ DFPC  VD EIG++I
Sbjct: 440 VLCFTSMA--RRAAVTSAAEVVKAAGGVGLIVAKNPSDALYPCSGDFPCVEVDYEIGTRI 499

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
            +YIR+  SP V+L  S+TI GNP+   +AYFSSRGPNS+APAILKPD+ APGV ILAAT
Sbjct: 500 LFYIRSNRSPAVKLSHSKTIVGNPLLPKVAYFSSRGPNSIAPAILKPDITAPGVNILAAT 559

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           SPLD   D G+ + +GTS+ATPHVS IVALLK+LHP WSPAAIKSA++TTAW  H  SG 
Sbjct: 560 SPLDRFEDNGYAMHSGTSMATPHVSGIVALLKALHPDWSPAAIKSALVTTAWRNH-PSGY 619

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
           PI  EGSP+K  +PFD+GGGI NPN AA+PGL+YDM TADYI Y C MGYNN+AIS LT 
Sbjct: 620 PIFAEGSPQKLANPFDFGGGIANPNGAADPGLLYDMDTADYIHYLCVMGYNNAAISRLTG 679

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             TECP+++  SILD+NLPS+T+P L    T+TRTVTN G   S+YR VI+PP G  V V
Sbjct: 680 QPTECPSKEP-SILDVNLPSLTIPNLRKSITLTRTVTNAGAPNSIYRAVIEPPFGIIVSV 739

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPF 716
           +P VL F+   +K++F VTI +  +++ G+ FGS+ W+DG+H+V+SPLSVR ++  P+
Sbjct: 740 KPDVLLFSNQTKKINFTVTINATHQVNTGYYFGSLSWTDGIHMVKSPLSVRTEMLQPY 787

BLAST of MS012766 vs. NCBI nr
Match: XP_024170229.1 (subtilisin-like protease SBT3.3 isoform X1 [Rosa chinensis] >PRQ16749.1 putative tripeptidyl-peptidase II [Rosa chinensis])

HSP 1 Score: 878.2 bits (2268), Expect = 4.7e-251
Identity = 439/717 (61.23%), Postives = 556/717 (77.55%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K  AS+  VYSY+H FSGFAAKLT++QA + AE+P V+RV+PN LY L TTRSWDFLG+S
Sbjct: 69  KSLASELMVYSYRHGFSGFAAKLTEAQAQECAELPDVVRVIPNTLYKLQTTRSWDFLGLS 128

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P   S N+L SSN+GDG+I+G++D+GIWPES++FN++GLGPVP  W+G+C+SGEKFNA+ 
Sbjct: 129 PHSPSSNILPSSNMGDGVIVGVLDTGIWPESKSFNEEGLGPVPSHWKGICESGEKFNATL 188

Query: 121 NCNRKIIGARWYVDGFKVEFGE-FNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYR 180
           +CNRKIIGARW+ DG   E+G+  N S  TEF+SPRD++GHGT TASTAAGSFV+NVSY+
Sbjct: 189 HCNRKIIGARWFNDGILAEYGQPLNTSERTEFMSPRDAHGHGTHTASTAAGSFVTNVSYK 248

Query: 181 GLAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           GL  GT+RGGAP ARLA+YK CW++   GGQC+ AD+LKA D+A+ D VDVLSLS+G SV
Sbjct: 249 GLGHGTVRGGAPNARLAVYKVCWNVL--GGQCSGADMLKAFDEAIHDGVDVLSLSIGGSV 308

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL+ +VDE + +A GSFHA++R I VVC+A NDGPS++TV NTSPWI+ VAA + DR F 
Sbjct: 309 PLYSDVDERDGIATGSFHAVARGITVVCAAANDGPSAQTVQNTSPWIITVAASTTDRAFP 368

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS-GLRQEVAGLCELVPLVNGSKWMAGKVV 360
            +I LGNN TFLGQ +F+G EIG++ L+YP S GL     G+CE + L      ++GKVV
Sbjct: 369 TSITLGNNKTFLGQAMFTGLEIGFTSLIYPESGGLEPTATGVCESLSL--NKTMVSGKVV 428

Query: 361 LCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIF 420
           LCF+        A++ A+ AVK   GVGLIVA++P D  + C+DDFPC  VD EIG++I 
Sbjct: 429 LCFTSLGRRR--AVTSASAAVKEAGGVGLIVAKNPTDGLYPCSDDFPCIEVDYEIGTRIV 488

Query: 421 YYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATS 480
           +YIR+T  PLV+L RS+TI G PI A +AYFSSRGPNS APAILKPD+AAPGV ILAATS
Sbjct: 489 FYIRSTRYPLVKLNRSKTIAGKPILARVAYFSSRGPNSAAPAILKPDIAAPGVNILAATS 548

Query: 481 PLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVP 540
           PLD   D G+ + +GTS+ATPHVS IVALLK+LHP WSPAAI+SA++T+AW  +  SG+P
Sbjct: 549 PLDSFVDAGYVMHSGTSMATPHVSGIVALLKALHPNWSPAAIRSALVTSAWR-NGPSGLP 608

Query: 541 ILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQD 600
           I  EGSP+K  +PFD+GGGIV+PNAAANPGL+YDM  ADYI Y C+MGYNNSAIS LT  
Sbjct: 609 IFAEGSPQKLTNPFDFGGGIVHPNAAANPGLVYDMGAADYIHYLCAMGYNNSAISRLTGQ 668

Query: 601 KTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVE 660
            T CP   + SILD+NLPSIT+P+L +  TVTRTVTNVG+  SVY   IDPP+GT V V+
Sbjct: 669 TTTCPIM-RPSILDINLPSITIPSLRHSITVTRTVTNVGDSKSVYEATIDPPLGTMVSVK 728

Query: 661 PPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPF 716
           P  L FN+TV+KL+F++TI +  +M+ G+ FGS+ W+D VH V+ PLSVR +    F
Sbjct: 729 PNPLVFNSTVQKLTFEITISTTHQMNTGYYFGSLTWTDRVHAVRIPLSVRTEFLQHF 777

BLAST of MS012766 vs. NCBI nr
Match: XP_030951705.1 (subtilisin-like protease SBT3.5 [Quercus lobata])

HSP 1 Score: 874.8 bits (2259), Expect = 5.2e-250
Identity = 438/711 (61.60%), Postives = 550/711 (77.36%), Query Frame = 0

Query: 4   ASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVSPFP 63
           AS   VYSYKH FSGFAAKLTKSQA +LAE+PGV+RV+PN L+ L TTRSWDFLG+S   
Sbjct: 70  ASDLMVYSYKHGFSGFAAKLTKSQAQKLAELPGVVRVIPNRLHKLQTTRSWDFLGLS--S 129

Query: 64  YSP-NLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASRNC 123
           +SP N+LHSSN+GDG IIG+ D+G+WPES  F D+GLGPVP RWRG+CKSGEKFNAS +C
Sbjct: 130 HSPNNILHSSNMGDGAIIGVFDTGLWPESRVFRDEGLGPVPPRWRGVCKSGEKFNASTHC 189

Query: 124 NRKIIGARWYVDGFKVEFGE-FNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRGL 183
           NRKIIGARWY++GF  E+G+  N S   EF+SPRD+NGHGT TASTA GSFV NVSY+GL
Sbjct: 190 NRKIIGARWYINGFLAEYGKPLNTSGDQEFLSPRDANGHGTHTASTAGGSFVGNVSYKGL 249

Query: 184 AGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVPL 243
             GT+RGGAPRARLA+YK CW +   GGQC++AD+LKA DDA+ D VDVLSLS+G S+PL
Sbjct: 250 GSGTVRGGAPRARLAIYKVCWDIL--GGQCSSADILKAFDDAIHDGVDVLSLSIGSSIPL 309

Query: 244 FPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLAT 303
           F +VDE + +A GSFHA++R I VVC A N+GPS+ TV NT+PWIL VAA +MDR F  +
Sbjct: 310 FSDVDERDGIATGSFHAVARGITVVCGAANEGPSAYTVHNTAPWILTVAASTMDRAFPTS 369

Query: 304 IVLGNNNTFLGQTLFSGEEIGYSGLVY-PGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC 363
           I LGNN T LGQ +F+G+EIG++ L+Y   +      AG+CE + L N S  ++GKVVLC
Sbjct: 370 ITLGNNKTLLGQAIFTGKEIGFTDLIYLESTKFDPNAAGMCEDLSLNNIS--VSGKVVLC 429

Query: 364 FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY 423
           F+        A+ +A+  VK   GVG+I+A+ P+D  ++C  DFPC  VD EIG++I +Y
Sbjct: 430 FTSIARRT--ALVQASGVVKEAGGVGVIIAKKPNDALYSCDGDFPCIEVDYEIGTRILFY 489

Query: 424 IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL 483
           IR+T SPLV+L  S+TI G P+S  +AYFSSRGPNS+APAILKPD+ APG  ILAATSPL
Sbjct: 490 IRSTRSPLVKLSPSKTIVGKPVSPKVAYFSSRGPNSIAPAILKPDITAPGANILAATSPL 549

Query: 484 DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVPIL 543
               D G+ + +GTS++TPH+S IVALLK LHP WSPAAIKSA+ITTAW T   SG+PI 
Sbjct: 550 GQIMDGGYAMLSGTSMSTPHISGIVALLKVLHPHWSPAAIKSALITTAWQT-GPSGLPIF 609

Query: 544 TEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT 603
            EG P+   +PFD+GGGIVN N AA+PGL+YDM T DYI Y C+M YNNSAIS LT   T
Sbjct: 610 AEGFPQNLANPFDFGGGIVNANGAADPGLVYDMGTTDYIHYLCAMAYNNSAISRLTGKST 669

Query: 604 ECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP 663
            CP+  ++SILD+NLPSIT+P+L N +T+TRTVTNVG L ++YR VIDPPIG  V V+P 
Sbjct: 670 VCPS-TKVSILDVNLPSITIPSLRNSSTITRTVTNVGGLKTIYRAVIDPPIGIMVSVKPD 729

Query: 664 VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQI 712
           VL FN+T++K+SFKVT+ +  ++D G+ FGS+ W++GVH V+SPLSVR ++
Sbjct: 730 VLVFNSTIKKISFKVTVSTTHKVDTGYYFGSLTWTNGVHAVRSPLSVRTEL 770

BLAST of MS012766 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 3.8e-219
Identity = 386/719 (53.69%), Postives = 524/719 (72.88%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K  A ++ VYSY+H FSGFAAKLT+SQA +LA+ P V+ V+ +  Y+L TTR+WD+LG+S
Sbjct: 60  KVDAHESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLS 119

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 NLL+ +N+GD +IIG ID+G+WPESE+FND+G+GP+P  W+G C+SGEKF  S 
Sbjct: 120 -VANPNNLLNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKF-IST 179

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCNRK+IGA+++++GF  E   FN + + ++IS RD  GHGT TAS A GSFV N+SY+G
Sbjct: 180 NCNRKLIGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKG 239

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPG-GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGG LRGGAPRAR+A+YKACW +   G   C+++D+LKA+D+++ D VDVLSLSLG  +
Sbjct: 240 LAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQI 299

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL+PE D  + +A G+FHA+++ I VVC+ GN GP+++TVLNT+PWI+ VAA ++DR+F 
Sbjct: 300 PLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFP 359

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYP-GSGLRQEV-AGLCELVPLVNGSKWMAGKV 360
             I LGN    LGQ L++G+E+G++ LVYP  +G   E  +G+CE + L N ++ MAGKV
Sbjct: 360 TPITLGNRKVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNL-NPNRTMAGKV 419

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+        A+SRAA  VKA  G+G+I+AR+P      C DDFPC  +D E+G+ +
Sbjct: 420 VLCFTTNTLFT--AVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDV 479

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             YIR+T SP+V++  SRT+ G P+   +A FSSRGPNS++PAILKPD+ APGV+ILAAT
Sbjct: 480 LLYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAAT 539

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           SP   ++  GF I  GTS+A P V+ +VALLK+LHP WSPAA +SAI+TTAW T    G 
Sbjct: 540 SPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRT-DPFGE 599

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGS RK  DPFDYGGGIVNP  AA+PGLIYDM   DYI Y CS GYN+S+I+ L  
Sbjct: 600 QIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVG 659

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
           + T C T  + S+LD+NLPSIT+P L +  T+TRTVTNVG + SVY+VV++PP+G +V V
Sbjct: 660 NVTVCST-PKTSVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVV 719

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+  + +SF V + +  +++ GF FG++IW+D +H V  P+SVR QI   ++
Sbjct: 720 APETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQILQNYY 771

BLAST of MS012766 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 8.4e-219
Identity = 385/719 (53.55%), Postives = 514/719 (71.49%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K+ A  + V+SY+H FSGFAAKLTKSQA +LA++P V+ V P+  Y L TTR+WD+LG+S
Sbjct: 61  KEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLS 120

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 NLL+ +N+G+ +IIGI+DSG+WPESE FND+G+GPVP  W+G C SGE F +S+
Sbjct: 121 -VANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQ 180

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
            CN+K+IGA+++++GF      FN + + +FISPRD +GHGT  A+ A GS+V ++SY+G
Sbjct: 181 -CNKKLIGAKYFINGFLATHESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSISYKG 240

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGG-GQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGGT+RGGAPRAR+AMYKACW L       C++AD+LKA+D+A+ D VDVLSLS+G   
Sbjct: 241 LAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRF 300

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           P FPE D   V+A G+FHA+ + I VVCS GN GP+++TV NT+PWIL VAA ++DR+F 
Sbjct: 301 PYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFP 360

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS--GLRQEVAGLCELVPLVNGSKWMAGKV 360
             I LGNN   LGQ +++G E+G++ LVYP +     +  +G CEL+   N +  MAGKV
Sbjct: 361 TPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELL-FFNSNHTMAGKV 420

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+ +     I +S A   VK   G+G+IVAR+P D    C DDFPC  VD E+G+ I
Sbjct: 421 VLCFTTSTRY--ITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDI 480

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             YIR+T  P+V++  S+T+ G P+   +A FSSRGPNS+ PAILKPD+AAPGV+ILAAT
Sbjct: 481 LLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAAT 540

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           +     NDRGF   +GTS+A P +S +VALLK+LH  WSPAAI+SAI+TTAW T    G 
Sbjct: 541 TTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRT-DPFGE 600

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGSPRK  DPFDYGGG+VNP  AA PGL+YD+   DY+ Y CS+GYN ++IS L  
Sbjct: 601 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 660

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             T C +  + S+LD NLPSIT+P L +  T+TRT+TNVG L SVY+VVI+PPIG +V V
Sbjct: 661 KGTVC-SNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTV 720

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+T +++SFKV + +  +++ G+ FGS+ WSD +H V  PLSVR QI   ++
Sbjct: 721 TPETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQILQNYY 772

BLAST of MS012766 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 755.7 bits (1950), Expect = 4.6e-217
Identity = 377/713 (52.88%), Postives = 518/713 (72.65%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K++A  + V+S++H FSGFAAKLT+SQA ++A++P V+ V+P+  Y   TTR+WD+LG+S
Sbjct: 65  KEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGLS 124

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P     NLL+ +N+G+ +IIGIIDSG+WPESE FND+ +GPVP  W+G C+SGE FN+S 
Sbjct: 125 P-TNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCESGEDFNSS- 184

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           +CN+K+IGA+++++ F      FN S + +FISPR  NGHGT  A+ A GS+V N SY+G
Sbjct: 185 HCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKG 244

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 240
           LAGGT+RGGAPRAR+A+YK CW L      C++AD+LKA+D+A+ D VDVLSLSLG   P
Sbjct: 245 LAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLGFE-P 304

Query: 241 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLA 300
           L+PE D  + +A G+FHA+ + I VVC+AGN GP+++TV NT+PWIL VAA ++DR+F+ 
Sbjct: 305 LYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVT 364

Query: 301 TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS--GLRQEVAGLCELVPLVNGSKWMAGKVV 360
            + LGNN   LGQ +++G E+G++ LVYP +     +  +G CE + L+N ++ MAGKVV
Sbjct: 365 PMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERL-LINSNRTMAGKVV 424

Query: 361 LCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIF 420
           LCF+   +   I+++RAA  VK   G+G+I+A  P ++   C DDFPC  VD E+G+ I 
Sbjct: 425 LCFTE--SPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYIL 484

Query: 421 YYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATS 480
           +YIR+  SP+V++  SRT+ G P+   +A FSSRGPN ++ AILKPD+AAPGV+ILAAT+
Sbjct: 485 FYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAATT 544

Query: 481 PLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVP 540
                NDRGF   +GTS+ATP +S IVALLK+LHP WSPAAI+SAI+TTAW T    G  
Sbjct: 545 TNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRT-DPFGEQ 604

Query: 541 ILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQD 600
           I  EGSPRK  DPFDYGGG+VNP  A  PGL+YD+   DY+ Y CS+GYN ++IS L   
Sbjct: 605 IFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGK 664

Query: 601 KTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVE 660
            T C +  + S+LD NLPSIT+P L    T+ RT+TNVG L SVYRV ++PP+GT+V V 
Sbjct: 665 GTVC-SYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQVTVT 724

Query: 661 PPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQI 712
           P  L FN+T +++SFKV++ +  +++ G+ FGS+ WSD +H V  PLSVR Q+
Sbjct: 725 PETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVRTQL 769

BLAST of MS012766 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.5e-215
Identity = 387/719 (53.82%), Postives = 516/719 (71.77%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           KK A  + VYSY+H FSGFAAKLTKSQA ++A++P V+ V+P+  ++L TTR+W++LG+S
Sbjct: 63  KKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLS 122

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 NLL+ +N+GD +IIG+ID+G+WPESE+FND+G+GP+PR+W+G C+SGE F  S 
Sbjct: 123 S-ANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR-ST 182

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           +CNRK+IGA+++++GF  E   FN + + ++IS RD +GHGT  AS A GSFV NVSY+G
Sbjct: 183 DCNRKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKG 242

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPG-GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGGTLRGGAPRAR+AMYKACW      G  C+ +D++KA+D+A+ D VDVLS+SL   +
Sbjct: 243 LAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQI 302

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL  E D  +  A G FHA+++ I VVC+ GNDGP+++TV+N +PWIL VAA ++DR+F 
Sbjct: 303 PLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFP 362

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGL--RQEVAGLCELVPLVNGSKWMAGKV 360
             I LGNN   LGQ  ++G E+G + LVYP +     +  +G+CE + L N +  MA KV
Sbjct: 363 TPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNL-NPNYTMAMKV 422

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+   +    AISRAA  VKA  G+GLI++R+P      C DDFPC  VD E+G+ I
Sbjct: 423 VLCFT--ASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDI 482

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             YIR+T SP+V++ RSRT++G P+   +  FSSRGPNS++PAILKPD+AAPGV ILAAT
Sbjct: 483 LSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAAT 542

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           SP D  N  GF + +GTS+ATP +S ++ALLK+LHP WSPAA +SAI+TTAW T    G 
Sbjct: 543 SPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRT-DPFGE 602

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGS RK  DPFDYGGGIVNP  AA PGLIYDM   DYI Y CS GYN+S+IS L  
Sbjct: 603 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 662

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             T C +  + S+LD+NLPSIT+P L +  T+TRTVTNVG + SVY+V ++PP+G RV V
Sbjct: 663 QITVC-SNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVV 722

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+    +SF V + +  +++ G+ FGS+ W+D VH V  PLSVR QI   ++
Sbjct: 723 TPETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQILQNYY 774

BLAST of MS012766 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 1.2e-214
Identity = 377/719 (52.43%), Postives = 511/719 (71.07%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K+ A+ + VYSY+H FSGFAAKLT+SQA ++A++P V+ V+P+  Y L TTR+WD+LG+S
Sbjct: 65  KEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS 124

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 +LLH +N+G+ IIIG+ID+G+WPESE FND G GPVP  W+G C++GE FN+S 
Sbjct: 125 A-ANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSS- 184

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCN+K+IGA+++++GF  E   FN + + +FISPRD +GHGT  ++ A GSFV N+SY+G
Sbjct: 185 NCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKG 244

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGG-GQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGGT+RGGAPRA +AMYKACW L       C++AD+LKA+D+A+ D VDVLS+SLG SV
Sbjct: 245 LAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSV 304

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL+ E D  + +  G+FHA+ + I VVCS GN GP S TV NT+PWI+ VAA ++DR+F 
Sbjct: 305 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 364

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS--GLRQEVAGLCELVPLVNGSKWMAGKV 360
             + LGNN   LGQ +++G  +G++ LVYP +     +  +G CE + L N ++ M GKV
Sbjct: 365 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEEL-LFNSNRTMEGKV 424

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+ +      A+  AA  VK   G+G+I+ARHP      C DDFPC  VD E+G+ I
Sbjct: 425 VLCFTTSPYGG--AVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDI 484

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             Y R++ SP+V++  S+T+ G P+   +A FSSRGPNS+APAILKPD+AAPGV+ILAAT
Sbjct: 485 LLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAAT 544

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           +     +D+GF + +GTS+A P +S + ALLK+LH  WSPAAI+SAI+TTAW T    G 
Sbjct: 545 TN-TTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKT-DPFGE 604

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGSP K  DPFDYGGG+VNP  +ANPGL+YDM   DY+ Y CS+GYN ++IS L  
Sbjct: 605 QIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIG 664

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             T C +  + S+LD NLPSIT+P L +  T+TRTVTNVG L SVYRV ++PP+G +V V
Sbjct: 665 KTTVC-SNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTV 724

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+T +K+ FKV + +  + + G+ FGS+ WSD +H V  PLSVR QI   ++
Sbjct: 725 TPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQILQNYY 775

BLAST of MS012766 vs. ExPASy TrEMBL
Match: A0A6J1C5D1 (subtilisin-like protease SBT3.5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008525 PE=3 SV=1)

HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 707/716 (98.74%), Postives = 708/716 (98.88%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS
Sbjct: 55  KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 114

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P PYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRG CKSGEKFNASR
Sbjct: 115 PSPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGTCKSGEKFNASR 174

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCNRKIIGARWYVDGFK EFGEFNRS ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG
Sbjct: 175 NCNRKIIGARWYVDGFKAEFGEFNRSSATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 234

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 240
           LAGGTLRGGAPRARLAMYKACWSLP GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP
Sbjct: 235 LAGGTLRGGAPRARLAMYKACWSLPSGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 294

Query: 241 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLA 300
           LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAA SMDRTFLA
Sbjct: 295 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAASSMDRTFLA 354

Query: 301 TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC 360
           TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC
Sbjct: 355 TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC 414

Query: 361 FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY 420
           FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY
Sbjct: 415 FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY 474

Query: 421 IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL 480
           IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL
Sbjct: 475 IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL 534

Query: 481 DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVPIL 540
           DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAI+TTAWNTHSSSGVPIL
Sbjct: 535 DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIVTTAWNTHSSSGVPIL 594

Query: 541 TEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT 600
           TEGSP KRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT
Sbjct: 595 TEGSPWKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT 654

Query: 601 ECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP 660
           ECPTQDQISILDLNLPSITVPALSNW TVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP
Sbjct: 655 ECPTQDQISILDLNLPSITVPALSNWNTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP 714

Query: 661 VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
           VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH
Sbjct: 715 VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 770

BLAST of MS012766 vs. ExPASy TrEMBL
Match: A0A6J1C8Y6 (subtilisin-like protease SBT3.5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008525 PE=3 SV=1)

HSP 1 Score: 1400.6 bits (3624), Expect = 0.0e+00
Identity = 707/726 (97.38%), Postives = 708/726 (97.52%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS
Sbjct: 55  KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 114

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P PYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRG CKSGEKFNASR
Sbjct: 115 PSPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGTCKSGEKFNASR 174

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCNRKIIGARWYVDGFK EFGEFNRS ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG
Sbjct: 175 NCNRKIIGARWYVDGFKAEFGEFNRSSATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 234

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 240
           LAGGTLRGGAPRARLAMYKACWSLP GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP
Sbjct: 235 LAGGTLRGGAPRARLAMYKACWSLPSGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 294

Query: 241 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLA 300
           LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAA SMDRTFLA
Sbjct: 295 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAASSMDRTFLA 354

Query: 301 TIVLGNNNTFL----------GQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGS 360
           TIVLGNNNTFL          GQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGS
Sbjct: 355 TIVLGNNNTFLVGLIWPYLLQGQTLFSGEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGS 414

Query: 361 KWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVD 420
           KWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVD
Sbjct: 415 KWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVD 474

Query: 421 LEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPG 480
           LEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPG
Sbjct: 475 LEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPG 534

Query: 481 VAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWN 540
           VAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAI+TTAWN
Sbjct: 535 VAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIVTTAWN 594

Query: 541 THSSSGVPILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNS 600
           THSSSGVPILTEGSP KRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNS
Sbjct: 595 THSSSGVPILTEGSPWKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNS 654

Query: 601 AISVLTQDKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPP 660
           AISVLTQDKTECPTQDQISILDLNLPSITVPALSNW TVTRTVTNVGNLTSVYRVVIDPP
Sbjct: 655 AISVLTQDKTECPTQDQISILDLNLPSITVPALSNWNTVTRTVTNVGNLTSVYRVVIDPP 714

Query: 661 IGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQ 717
           IGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQ
Sbjct: 715 IGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQ 774

BLAST of MS012766 vs. ExPASy TrEMBL
Match: A0A2P6P495 (Putative tripeptidyl-peptidase II OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0187621 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 2.3e-251
Identity = 439/717 (61.23%), Postives = 556/717 (77.55%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K  AS+  VYSY+H FSGFAAKLT++QA + AE+P V+RV+PN LY L TTRSWDFLG+S
Sbjct: 69  KSLASELMVYSYRHGFSGFAAKLTEAQAQECAELPDVVRVIPNTLYKLQTTRSWDFLGLS 128

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P   S N+L SSN+GDG+I+G++D+GIWPES++FN++GLGPVP  W+G+C+SGEKFNA+ 
Sbjct: 129 PHSPSSNILPSSNMGDGVIVGVLDTGIWPESKSFNEEGLGPVPSHWKGICESGEKFNATL 188

Query: 121 NCNRKIIGARWYVDGFKVEFGE-FNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYR 180
           +CNRKIIGARW+ DG   E+G+  N S  TEF+SPRD++GHGT TASTAAGSFV+NVSY+
Sbjct: 189 HCNRKIIGARWFNDGILAEYGQPLNTSERTEFMSPRDAHGHGTHTASTAAGSFVTNVSYK 248

Query: 181 GLAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           GL  GT+RGGAP ARLA+YK CW++   GGQC+ AD+LKA D+A+ D VDVLSLS+G SV
Sbjct: 249 GLGHGTVRGGAPNARLAVYKVCWNVL--GGQCSGADMLKAFDEAIHDGVDVLSLSIGGSV 308

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL+ +VDE + +A GSFHA++R I VVC+A NDGPS++TV NTSPWI+ VAA + DR F 
Sbjct: 309 PLYSDVDERDGIATGSFHAVARGITVVCAAANDGPSAQTVQNTSPWIITVAASTTDRAFP 368

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS-GLRQEVAGLCELVPLVNGSKWMAGKVV 360
            +I LGNN TFLGQ +F+G EIG++ L+YP S GL     G+CE + L      ++GKVV
Sbjct: 369 TSITLGNNKTFLGQAMFTGLEIGFTSLIYPESGGLEPTATGVCESLSL--NKTMVSGKVV 428

Query: 361 LCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIF 420
           LCF+        A++ A+ AVK   GVGLIVA++P D  + C+DDFPC  VD EIG++I 
Sbjct: 429 LCFTSLGRRR--AVTSASAAVKEAGGVGLIVAKNPTDGLYPCSDDFPCIEVDYEIGTRIV 488

Query: 421 YYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATS 480
           +YIR+T  PLV+L RS+TI G PI A +AYFSSRGPNS APAILKPD+AAPGV ILAATS
Sbjct: 489 FYIRSTRYPLVKLNRSKTIAGKPILARVAYFSSRGPNSAAPAILKPDIAAPGVNILAATS 548

Query: 481 PLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVP 540
           PLD   D G+ + +GTS+ATPHVS IVALLK+LHP WSPAAI+SA++T+AW  +  SG+P
Sbjct: 549 PLDSFVDAGYVMHSGTSMATPHVSGIVALLKALHPNWSPAAIRSALVTSAWR-NGPSGLP 608

Query: 541 ILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQD 600
           I  EGSP+K  +PFD+GGGIV+PNAAANPGL+YDM  ADYI Y C+MGYNNSAIS LT  
Sbjct: 609 IFAEGSPQKLTNPFDFGGGIVHPNAAANPGLVYDMGAADYIHYLCAMGYNNSAISRLTGQ 668

Query: 601 KTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVE 660
            T CP   + SILD+NLPSIT+P+L +  TVTRTVTNVG+  SVY   IDPP+GT V V+
Sbjct: 669 TTTCPIM-RPSILDINLPSITIPSLRHSITVTRTVTNVGDSKSVYEATIDPPLGTMVSVK 728

Query: 661 PPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPF 716
           P  L FN+TV+KL+F++TI +  +M+ G+ FGS+ W+D VH V+ PLSVR +    F
Sbjct: 729 PNPLVFNSTVQKLTFEITISTTHQMNTGYYFGSLTWTDRVHAVRIPLSVRTEFLQHF 777

BLAST of MS012766 vs. ExPASy TrEMBL
Match: A0A7N2KV16 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 2.5e-250
Identity = 438/711 (61.60%), Postives = 550/711 (77.36%), Query Frame = 0

Query: 4   ASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVSPFP 63
           AS   VYSYKH FSGFAAKLTKSQA +LAE+PGV+RV+PN L+ L TTRSWDFLG+S   
Sbjct: 70  ASDLMVYSYKHGFSGFAAKLTKSQAQKLAELPGVVRVIPNRLHKLQTTRSWDFLGLS--S 129

Query: 64  YSP-NLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASRNC 123
           +SP N+LHSSN+GDG IIG+ D+G+WPES  F D+GLGPVP RWRG+CKSGEKFNAS +C
Sbjct: 130 HSPNNILHSSNMGDGAIIGVFDTGLWPESRVFRDEGLGPVPPRWRGVCKSGEKFNASTHC 189

Query: 124 NRKIIGARWYVDGFKVEFGE-FNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRGL 183
           NRKIIGARWY++GF  E+G+  N S   EF+SPRD+NGHGT TASTA GSFV NVSY+GL
Sbjct: 190 NRKIIGARWYINGFLAEYGKPLNTSGDQEFLSPRDANGHGTHTASTAGGSFVGNVSYKGL 249

Query: 184 AGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVPL 243
             GT+RGGAPRARLA+YK CW +   GGQC++AD+LKA DDA+ D VDVLSLS+G S+PL
Sbjct: 250 GSGTVRGGAPRARLAIYKVCWDIL--GGQCSSADILKAFDDAIHDGVDVLSLSIGSSIPL 309

Query: 244 FPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLAT 303
           F +VDE + +A GSFHA++R I VVC A N+GPS+ TV NT+PWIL VAA +MDR F  +
Sbjct: 310 FSDVDERDGIATGSFHAVARGITVVCGAANEGPSAYTVHNTAPWILTVAASTMDRAFPTS 369

Query: 304 IVLGNNNTFLGQTLFSGEEIGYSGLVY-PGSGLRQEVAGLCELVPLVNGSKWMAGKVVLC 363
           I LGNN T LGQ +F+G+EIG++ L+Y   +      AG+CE + L N S  ++GKVVLC
Sbjct: 370 ITLGNNKTLLGQAIFTGKEIGFTDLIYLESTKFDPNAAGMCEDLSLNNIS--VSGKVVLC 429

Query: 364 FSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYY 423
           F+        A+ +A+  VK   GVG+I+A+ P+D  ++C  DFPC  VD EIG++I +Y
Sbjct: 430 FTSIARRT--ALVQASGVVKEAGGVGVIIAKKPNDALYSCDGDFPCIEVDYEIGTRILFY 489

Query: 424 IRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPL 483
           IR+T SPLV+L  S+TI G P+S  +AYFSSRGPNS+APAILKPD+ APG  ILAATSPL
Sbjct: 490 IRSTRSPLVKLSPSKTIVGKPVSPKVAYFSSRGPNSIAPAILKPDITAPGANILAATSPL 549

Query: 484 DPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVPIL 543
               D G+ + +GTS++TPH+S IVALLK LHP WSPAAIKSA+ITTAW T   SG+PI 
Sbjct: 550 GQIMDGGYAMLSGTSMSTPHISGIVALLKVLHPHWSPAAIKSALITTAWQT-GPSGLPIF 609

Query: 544 TEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKT 603
            EG P+   +PFD+GGGIVN N AA+PGL+YDM T DYI Y C+M YNNSAIS LT   T
Sbjct: 610 AEGFPQNLANPFDFGGGIVNANGAADPGLVYDMGTTDYIHYLCAMAYNNSAISRLTGKST 669

Query: 604 ECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPP 663
            CP+  ++SILD+NLPSIT+P+L N +T+TRTVTNVG L ++YR VIDPPIG  V V+P 
Sbjct: 670 VCPS-TKVSILDVNLPSITIPSLRNSSTITRTVTNVGGLKTIYRAVIDPPIGIMVSVKPD 729

Query: 664 VLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQI 712
           VL FN+T++K+SFKVT+ +  ++D G+ FGS+ W++GVH V+SPLSVR ++
Sbjct: 730 VLVFNSTIKKISFKVTVSTTHKVDTGYYFGSLTWTNGVHAVRSPLSVRTEL 770

BLAST of MS012766 vs. ExPASy TrEMBL
Match: A0A5N5JW32 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_026149 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.6e-249
Identity = 432/718 (60.17%), Postives = 557/718 (77.58%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K+ AS+  VYSYKH FSGFAAK T+SQA ++AE+PGV+RV+PN L+ + TTRSWDFLG+S
Sbjct: 65  KEIASELMVYSYKHGFSGFAAKFTESQAQKVAELPGVVRVIPNSLHRIQTTRSWDFLGLS 124

Query: 61  PFPYSP-NLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNAS 120
              +SP N LH+S++GDG+IIG++D+GIWPE+++F+D GLGP+P  W+G+C+SG++F A 
Sbjct: 125 --AHSPVNTLHNSSMGDGVIIGVLDTGIWPEAKSFSDKGLGPIPSHWKGVCESGKRFKAK 184

Query: 121 RNCNRKIIGARWYVDGFKVEFGE-FNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSY 180
            +CNRKIIGARW+V+G   E+G+  N S   EF SPRD+NGHGT TASTAAG+FV NVSY
Sbjct: 185 SHCNRKIIGARWFVEGLVAEYGQPLNISGNREFFSPRDANGHGTHTASTAAGTFVHNVSY 244

Query: 181 RGLAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKS 240
           RGL  GT+RGGAPRARLA+YK CW++   GGQC++AD+LKA D+A+ D VDVLSLS+G S
Sbjct: 245 RGLGHGTIRGGAPRARLAIYKVCWNVL--GGQCSSADILKAFDEAIHDGVDVLSLSIGSS 304

Query: 241 VPLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTF 300
           +PLF ++DE N +A GSFHA+++ I VVC A NDGP ++TV N++PWIL VAA SMDR F
Sbjct: 305 IPLFSDIDERNGIATGSFHAVAKGITVVCGAANDGPFAQTVQNSAPWILTVAASSMDRAF 364

Query: 301 LATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS-GLRQEVAGLCELVPLVNGSKWMAGKV 360
              I LGNN TFLGQ +++G+EIG+S L+YP + GL    AG+C+ + + N    +AGKV
Sbjct: 365 PTPITLGNNKTFLGQAIYTGKEIGFSSLIYPETKGLNPNSAGVCQSLSVDN--SMVAGKV 424

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+    MN  A+  AAE VK   GVGLIVA++P +  + C D FPC  VD EIG++I
Sbjct: 425 VLCFT---TMNLGAVRSAAEVVKEAGGVGLIVAKNPSEALYPCTDGFPCVEVDYEIGTRI 484

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
            +YIR+T SP+V+L  S+TI G P+ A +A+FSSRGPNS+APAILKPD+AAPGV ILAAT
Sbjct: 485 LFYIRSTRSPVVKLSPSKTIVGEPVLAKVAHFSSRGPNSIAPAILKPDIAAPGVNILAAT 544

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           SPLD   D G+ + +GTS+ATPHVS I ALLK++HP WSPAAIKSAI+TTAW  ++ SG 
Sbjct: 545 SPLDRFQDGGYAMHSGTSMATPHVSGIAALLKAMHPDWSPAAIKSAIVTTAWR-NNPSGF 604

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
           P+  EGSP+K  DPFDYGGGI NPN AA PGL+YDMAT DY++Y C+M YNN+AIS LT 
Sbjct: 605 PVFAEGSPQKLADPFDYGGGITNPNDAAQPGLVYDMATDDYVNYLCAMDYNNTAISRLTG 664

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
           + T CPT+   SIL++NLPSIT+P L N TT+TRTVTNVG   S+YR VI+PP GT V V
Sbjct: 665 NPTVCPTEGP-SILNINLPSITIPNLRNSTTLTRTVTNVGASNSIYRAVIEPPFGTSVSV 724

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPF 716
           EP VL F+   +KL+F VT+ +  +++ G+ FGSI W+DGVH V++PLSVR +I  P+
Sbjct: 725 EPNVLVFDRKTKKLTFSVTVNAAHQVNTGYFFGSITWTDGVHTVRTPLSVRTEISQPY 771

BLAST of MS012766 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 762.7 bits (1968), Expect = 2.7e-220
Identity = 386/719 (53.69%), Postives = 524/719 (72.88%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K  A ++ VYSY+H FSGFAAKLT+SQA +LA+ P V+ V+ +  Y+L TTR+WD+LG+S
Sbjct: 60  KVDAHESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLS 119

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 NLL+ +N+GD +IIG ID+G+WPESE+FND+G+GP+P  W+G C+SGEKF  S 
Sbjct: 120 -VANPNNLLNDTNMGDQVIIGFIDTGVWPESESFNDNGVGPIPSHWKGGCESGEKF-IST 179

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCNRK+IGA+++++GF  E   FN + + ++IS RD  GHGT TAS A GSFV N+SY+G
Sbjct: 180 NCNRKLIGAKYFINGFLAENEGFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKG 239

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPG-GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGG LRGGAPRAR+A+YKACW +   G   C+++D+LKA+D+++ D VDVLSLSLG  +
Sbjct: 240 LAGGNLRGGAPRARIAIYKACWYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQI 299

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL+PE D  + +A G+FHA+++ I VVC+ GN GP+++TVLNT+PWI+ VAA ++DR+F 
Sbjct: 300 PLYPETDLRDRIATGAFHAVAKGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFP 359

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYP-GSGLRQEV-AGLCELVPLVNGSKWMAGKV 360
             I LGN    LGQ L++G+E+G++ LVYP  +G   E  +G+CE + L N ++ MAGKV
Sbjct: 360 TPITLGNRKVILGQALYTGQELGFTSLVYPENAGFTNETFSGVCERLNL-NPNRTMAGKV 419

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+        A+SRAA  VKA  G+G+I+AR+P      C DDFPC  +D E+G+ +
Sbjct: 420 VLCFTTNTLFT--AVSRAASYVKAAGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDV 479

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             YIR+T SP+V++  SRT+ G P+   +A FSSRGPNS++PAILKPD+ APGV+ILAAT
Sbjct: 480 LLYIRSTRSPVVKIQPSRTLVGQPVGTKVATFSSRGPNSISPAILKPDIGAPGVSILAAT 539

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           SP   ++  GF I  GTS+A P V+ +VALLK+LHP WSPAA +SAI+TTAW T    G 
Sbjct: 540 SPDSNSSVGGFDILAGTSMAAPVVAGVVALLKALHPNWSPAAFRSAIVTTAWRT-DPFGE 599

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGS RK  DPFDYGGGIVNP  AA+PGLIYDM   DYI Y CS GYN+S+I+ L  
Sbjct: 600 QIFAEGSSRKVADPFDYGGGIVNPEKAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVG 659

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
           + T C T  + S+LD+NLPSIT+P L +  T+TRTVTNVG + SVY+VV++PP+G +V V
Sbjct: 660 NVTVCST-PKTSVLDVNLPSITIPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVV 719

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+  + +SF V + +  +++ GF FG++IW+D +H V  P+SVR QI   ++
Sbjct: 720 APETLVFNSKTKNVSFTVRVSTTHKINTGFYFGNLIWTDSMHNVTIPVSVRTQILQNYY 771

BLAST of MS012766 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 761.5 bits (1965), Expect = 5.9e-220
Identity = 385/719 (53.55%), Postives = 514/719 (71.49%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K+ A  + V+SY+H FSGFAAKLTKSQA +LA++P V+ V P+  Y L TTR+WD+LG+S
Sbjct: 61  KEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLS 120

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 NLL+ +N+G+ +IIGI+DSG+WPESE FND+G+GPVP  W+G C SGE F +S+
Sbjct: 121 -VANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQ 180

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
            CN+K+IGA+++++GF      FN + + +FISPRD +GHGT  A+ A GS+V ++SY+G
Sbjct: 181 -CNKKLIGAKYFINGFLATHESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSISYKG 240

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGG-GQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGGT+RGGAPRAR+AMYKACW L       C++AD+LKA+D+A+ D VDVLSLS+G   
Sbjct: 241 LAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRF 300

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           P FPE D   V+A G+FHA+ + I VVCS GN GP+++TV NT+PWIL VAA ++DR+F 
Sbjct: 301 PYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFP 360

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS--GLRQEVAGLCELVPLVNGSKWMAGKV 360
             I LGNN   LGQ +++G E+G++ LVYP +     +  +G CEL+   N +  MAGKV
Sbjct: 361 TPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELL-FFNSNHTMAGKV 420

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+ +     I +S A   VK   G+G+IVAR+P D    C DDFPC  VD E+G+ I
Sbjct: 421 VLCFTTSTRY--ITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTDI 480

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             YIR+T  P+V++  S+T+ G P+   +A FSSRGPNS+ PAILKPD+AAPGV+ILAAT
Sbjct: 481 LLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAAT 540

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           +     NDRGF   +GTS+A P +S +VALLK+LH  WSPAAI+SAI+TTAW T    G 
Sbjct: 541 TTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRT-DPFGE 600

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGSPRK  DPFDYGGG+VNP  AA PGL+YD+   DY+ Y CS+GYN ++IS L  
Sbjct: 601 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 660

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             T C +  + S+LD NLPSIT+P L +  T+TRT+TNVG L SVY+VVI+PPIG +V V
Sbjct: 661 KGTVC-SNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTV 720

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+T +++SFKV + +  +++ G+ FGS+ WSD +H V  PLSVR QI   ++
Sbjct: 721 TPETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQILQNYY 772

BLAST of MS012766 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 755.7 bits (1950), Expect = 3.3e-218
Identity = 377/713 (52.88%), Postives = 518/713 (72.65%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K++A  + V+S++H FSGFAAKLT+SQA ++A++P V+ V+P+  Y   TTR+WD+LG+S
Sbjct: 53  KEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDYLGLS 112

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
           P     NLL+ +N+G+ +IIGIIDSG+WPESE FND+ +GPVP  W+G C+SGE FN+S 
Sbjct: 113 P-TNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCESGEDFNSS- 172

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           +CN+K+IGA+++++ F      FN S + +FISPR  NGHGT  A+ A GS+V N SY+G
Sbjct: 173 HCNKKLIGAKYFINAFLATHESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKG 232

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGGGQCAAADVLKALDDAVQDRVDVLSLSLGKSVP 240
           LAGGT+RGGAPRAR+A+YK CW L      C++AD+LKA+D+A+ D VDVLSLSLG   P
Sbjct: 233 LAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLGFE-P 292

Query: 241 LFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFLA 300
           L+PE D  + +A G+FHA+ + I VVC+AGN GP+++TV NT+PWIL VAA ++DR+F+ 
Sbjct: 293 LYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVT 352

Query: 301 TIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS--GLRQEVAGLCELVPLVNGSKWMAGKVV 360
            + LGNN   LGQ +++G E+G++ LVYP +     +  +G CE + L+N ++ MAGKVV
Sbjct: 353 PMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERL-LINSNRTMAGKVV 412

Query: 361 LCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIF 420
           LCF+   +   I+++RAA  VK   G+G+I+A  P ++   C DDFPC  VD E+G+ I 
Sbjct: 413 LCFTE--SPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYIL 472

Query: 421 YYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATS 480
           +YIR+  SP+V++  SRT+ G P+   +A FSSRGPN ++ AILKPD+AAPGV+ILAAT+
Sbjct: 473 FYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAATT 532

Query: 481 PLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGVP 540
                NDRGF   +GTS+ATP +S IVALLK+LHP WSPAAI+SAI+TTAW T    G  
Sbjct: 533 TNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRT-DPFGEQ 592

Query: 541 ILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQD 600
           I  EGSPRK  DPFDYGGG+VNP  A  PGL+YD+   DY+ Y CS+GYN ++IS L   
Sbjct: 593 IFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGK 652

Query: 601 KTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVE 660
            T C +  + S+LD NLPSIT+P L    T+ RT+TNVG L SVYRV ++PP+GT+V V 
Sbjct: 653 GTVC-SYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQVTVT 712

Query: 661 PPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQI 712
           P  L FN+T +++SFKV++ +  +++ G+ FGS+ WSD +H V  PLSVR Q+
Sbjct: 713 PETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVRTQL 757

BLAST of MS012766 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 750.7 bits (1937), Expect = 1.0e-216
Identity = 387/719 (53.82%), Postives = 516/719 (71.77%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           KK A  + VYSY+H FSGFAAKLTKSQA ++A++P V+ V+P+  ++L TTR+W++LG+S
Sbjct: 63  KKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLS 122

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 NLL+ +N+GD +IIG+ID+G+WPESE+FND+G+GP+PR+W+G C+SGE F  S 
Sbjct: 123 S-ANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR-ST 182

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           +CNRK+IGA+++++GF  E   FN + + ++IS RD +GHGT  AS A GSFV NVSY+G
Sbjct: 183 DCNRKLIGAKYFINGFLAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKG 242

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPG-GGGQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGGTLRGGAPRAR+AMYKACW      G  C+ +D++KA+D+A+ D VDVLS+SL   +
Sbjct: 243 LAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQI 302

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL  E D  +  A G FHA+++ I VVC+ GNDGP+++TV+N +PWIL VAA ++DR+F 
Sbjct: 303 PLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFP 362

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGSGL--RQEVAGLCELVPLVNGSKWMAGKV 360
             I LGNN   LGQ  ++G E+G + LVYP +     +  +G+CE + L N +  MA KV
Sbjct: 363 TPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNL-NPNYTMAMKV 422

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+   +    AISRAA  VKA  G+GLI++R+P      C DDFPC  VD E+G+ I
Sbjct: 423 VLCFT--ASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDI 482

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             YIR+T SP+V++ RSRT++G P+   +  FSSRGPNS++PAILKPD+AAPGV ILAAT
Sbjct: 483 LSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAAT 542

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           SP D  N  GF + +GTS+ATP +S ++ALLK+LHP WSPAA +SAI+TTAW T    G 
Sbjct: 543 SPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRT-DPFGE 602

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGS RK  DPFDYGGGIVNP  AA PGLIYDM   DYI Y CS GYN+S+IS L  
Sbjct: 603 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 662

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             T C +  + S+LD+NLPSIT+P L +  T+TRTVTNVG + SVY+V ++PP+G RV V
Sbjct: 663 QITVC-SNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVV 722

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+    +SF V + +  +++ G+ FGS+ W+D VH V  PLSVR QI   ++
Sbjct: 723 TPETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQILQNYY 774

BLAST of MS012766 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 747.7 bits (1929), Expect = 8.9e-216
Identity = 377/719 (52.43%), Postives = 511/719 (71.07%), Query Frame = 0

Query: 1   KKKASKATVYSYKHAFSGFAAKLTKSQAHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVS 60
           K+ A+ + VYSY+H FSGFAAKLT+SQA ++A++P V+ V+P+  Y L TTR+WD+LG+S
Sbjct: 65  KEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS 124

Query: 61  PFPYSPNLLHSSNLGDGIIIGIIDSGIWPESEAFNDDGLGPVPRRWRGMCKSGEKFNASR 120
                 +LLH +N+G+ IIIG+ID+G+WPESE FND G GPVP  W+G C++GE FN+S 
Sbjct: 125 A-ANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSS- 184

Query: 121 NCNRKIIGARWYVDGFKVEFGEFNRSVATEFISPRDSNGHGTQTASTAAGSFVSNVSYRG 180
           NCN+K+IGA+++++GF  E   FN + + +FISPRD +GHGT  ++ A GSFV N+SY+G
Sbjct: 185 NCNKKLIGAKYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKG 244

Query: 181 LAGGTLRGGAPRARLAMYKACWSLPGGG-GQCAAADVLKALDDAVQDRVDVLSLSLGKSV 240
           LAGGT+RGGAPRA +AMYKACW L       C++AD+LKA+D+A+ D VDVLS+SLG SV
Sbjct: 245 LAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSV 304

Query: 241 PLFPEVDEENVVAIGSFHAISRNIPVVCSAGNDGPSSETVLNTSPWILNVAAGSMDRTFL 300
           PL+ E D  + +  G+FHA+ + I VVCS GN GP S TV NT+PWI+ VAA ++DR+F 
Sbjct: 305 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 364

Query: 301 ATIVLGNNNTFLGQTLFSGEEIGYSGLVYPGS--GLRQEVAGLCELVPLVNGSKWMAGKV 360
             + LGNN   LGQ +++G  +G++ LVYP +     +  +G CE + L N ++ M GKV
Sbjct: 365 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEEL-LFNSNRTMEGKV 424

Query: 361 VLCFSRAVAMNPIAISRAAEAVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKI 420
           VLCF+ +      A+  AA  VK   G+G+I+ARHP      C DDFPC  VD E+G+ I
Sbjct: 425 VLCFTTSPYGG--AVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDI 484

Query: 421 FYYIRATSSPLVRLGRSRTITGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAAT 480
             Y R++ SP+V++  S+T+ G P+   +A FSSRGPNS+APAILKPD+AAPGV+ILAAT
Sbjct: 485 LLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAAT 544

Query: 481 SPLDPTNDRGFTIQTGTSIATPHVSAIVALLKSLHPTWSPAAIKSAIITTAWNTHSSSGV 540
           +     +D+GF + +GTS+A P +S + ALLK+LH  WSPAAI+SAI+TTAW T    G 
Sbjct: 545 TN-TTFSDQGFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKT-DPFGE 604

Query: 541 PILTEGSPRKRGDPFDYGGGIVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQ 600
            I  EGSP K  DPFDYGGG+VNP  +ANPGL+YDM   DY+ Y CS+GYN ++IS L  
Sbjct: 605 QIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIG 664

Query: 601 DKTECPTQDQISILDLNLPSITVPALSNWTTVTRTVTNVGNLTSVYRVVIDPPIGTRVEV 660
             T C +  + S+LD NLPSIT+P L +  T+TRTVTNVG L SVYRV ++PP+G +V V
Sbjct: 665 KTTVC-SNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTV 724

Query: 661 EPPVLAFNATVRKLSFKVTIWSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQIQSPFH 717
            P  L FN+T +K+ FKV + +  + + G+ FGS+ WSD +H V  PLSVR QI   ++
Sbjct: 725 TPETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQILQNYY 775