MS012764 (gene) Bitter gourd (TR) v1

Overview


Name	MS012764
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	subtilisin-like protease SBT3.9
Location	scaffold63: 3273972 .. 3275264 (+)
RNA-Seq Expression	MS012764
Synteny	MS012764

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGTTCACATCGTTTACTTGGGAGAAACTCAACACGAAGAGAGTAAATTGACAACTGAGTCTCACCATGAATTACTGTCCACTGTACTGGGAAGGTAATTAATCTACAGCTCAGAATTTTTAATGAAAAAAAAAAAAAGGTTTAGTGTTAATTATATTATGATATTTGATAATGAAGCAAGGAGAAGTCGTGGGAATCAATGGTGTACAGCTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTTACCAAGTCTCAGGCTCAAAAGCTAGCTGGTAAATATATTAATATATAAAAAAACATAATGAATTAATTTGAGAAGAAACTTTCAAATATTTCTATTTAATTCCTGTGTCTAAACTCTTGTCCCTATATTTAAAAAGATTAGGTAAATTTCTTATGTACTTTTAAGATCATATTGTTCATCTCATTCTATGTTTTAAAAAGTTTGTAATGTCTTTAAATTTTTGGATCTTAAAAAAGTTTATGATAAATTTTTAACTTGAGCTTTTCTAGGCTAACTAATCTGAGTTGGATTCAATTTTTAGATAACTGAATTTTTTGAACTCTAATACTATATCAATATTCATAAAATCTGTTTTCAAAACAAGGGAAGAATAACTCATATATATTATAAATCTTTGTTAGATTTCTTTTATATTTTCAACCTGTGATATATGCTTAAGAATAGTTTTAAAAGTATTATATTAAAATTAATTCAGAAAATTAAAGAAGTAAACTTGTAATTTAAGCCTCATCACTAAAACAAACAATTTGCGGTGGTACGAACGAATTGAACTTCAGAAATGGTGGGCGTGGTTCGAGTTCTTCCAAATTCGATTTACAAAATGCAGACAACAAGAAGTTGGGATTTCCTGGGGCTCTCAACTTCTCCCTCACAATCCTCCAACCTTCTTCATCGCGGTAAAATGGGCGAAGATGTCATTTAAAAAATTGAATGTATCATTATTATTTTTGAAAAAAATTGTAAAATCTAGATGTTATTATTTTTTTTTTCCTAATAATCAGGAATCTGGCCGGAGTCGGAGGCTTTTGGAGATGAAGGAATGGGGCGGGTGCCATCAAGATGGAAAGGCAAGTGCGAATCAGGAGAAGAATTCAACTCCACAAACTGCAACAGAAAAATCATAAGCGCGCGTTGGTACAATAAAGGCTTCATTGCTGACGTGGGACAGGAGGCACTGGCGGAGGAGAACTCATCCGCAAGGGACTTCAACGGACACGGAACCCACACCGCCAGCACAGCCGCAGGCTCCTTGGTGCCCAATGTC

mRNA sequence

CAGGTTCACATCGTTTACTTGGGAGAAACTCAACACGAAGAGAGTAAATTGACAACTGAGTCTCACCATGAATTACTGTCCACTGTACTGGGAAGCAAGGAGAAGTCGTGGGAATCAATGGTGTACAGCTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTTACCAAGTCTCAGGCTCAAAAGCTAGCTGAAATGGTGGGCGTGGTTCGAGTTCTTCCAAATTCGATTTACAAAATGCAGACAACAAGAAGTTGGGATTTCCTGGGGCTCTCAACTTCTCCCTCACAATCCTCCAACCTTCTTCATCGCGGAATCTGGCCGGAGTCGGAGGCTTTTGGAGATGAAGGAATGGGGCGGGTGCCATCAAGATGGAAAGGCAAGTGCGAATCAGGAGAAGAATTCAACTCCACAAACTGCAACAGAAAAATCATAAGCGCGCGTTGGTACAATAAAGGCTTCATTGCTGACGTGGGACAGGAGGCACTGGCGGAGGAGAACTCATCCGCAAGGGACTTCAACGGACACGGAACCCACACCGCCAGCACAGCCGCAGGCTCCTTGGTGCCCAATGTC

Coding sequence (CDS)

CAGGTTCACATCGTTTACTTGGGAGAAACTCAACACGAAGAGAGTAAATTGACAACTGAGTCTCACCATGAATTACTGTCCACTGTACTGGGAAGCAAGGAGAAGTCGTGGGAATCAATGGTGTACAGCTATAGACATGGCTTTTCTGGGTTTGCAGCCAAGCTTACCAAGTCTCAGGCTCAAAAGCTAGCTGAAATGGTGGGCGTGGTTCGAGTTCTTCCAAATTCGATTTACAAAATGCAGACAACAAGAAGTTGGGATTTCCTGGGGCTCTCAACTTCTCCCTCACAATCCTCCAACCTTCTTCATCGCGGAATCTGGCCGGAGTCGGAGGCTTTTGGAGATGAAGGAATGGGGCGGGTGCCATCAAGATGGAAAGGCAAGTGCGAATCAGGAGAAGAATTCAACTCCACAAACTGCAACAGAAAAATCATAAGCGCGCGTTGGTACAATAAAGGCTTCATTGCTGACGTGGGACAGGAGGCACTGGCGGAGGAGAACTCATCCGCAAGGGACTTCAACGGACACGGAACCCACACCGCCAGCACAGCCGCAGGCTCCTTGGTGCCCAATGTC

Protein sequence

QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQAQKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHRGIWPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEENSSARDFNGHGTHTASTAAGSLVPNV

Homology

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BLAST of MS012764 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 292.7 bits (748), Expect = 2.2e-75
Identity = 142/206 (68.93%), Postives = 170/206 (82.52%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  H ++K T +SHH+LLST+LGSKEKS E+MVYSY+HGFSGFAAKLTKSQA
Sbjct: 34  KVHIVYLGEKPHHDTKFTIDSHHQLLSTILGSKEKSMEAMVYSYKHGFSGFAAKLTKSQA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           QKL+EM  VVRV+P+S+YK+ TTRSWDFLGLS+SP +SSNLLHR              GI
Sbjct: 94  QKLSEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPFESSNLLHRAQMGENVIIGVIDTGI 153

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE+F D+G+G +PSRWKG CESGE+FNSTNCN+KII ARW+ KGF+AD+G++ALA+E
Sbjct: 154 WPESESFKDKGVGSIPSRWKGTCESGEQFNSTNCNKKIIGARWFMKGFVADLGRDALAKE 213

Query: 181 NSSARDFNGHGTHTASTAAGSLVPNV 193
             S RD NGHGTHTAS AAGS V N+
Sbjct: 214 YLSPRDLNGHGTHTASIAAGSFVANI 239

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BLAST of MS012764 vs. NCBI nr
Match: XP_008452040.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo])

HSP 1 Score: 291.2 bits (744), Expect = 6.5e-75
Identity = 141/205 (68.78%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  H ++K TT+SHH+LL+ +LGSKEKS E+MVYSY+HGFSGFAAKLTKS+A
Sbjct: 34  KVHIVYLGEKPHHDTKFTTDSHHQLLAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR-------------GIW 120
           QKL+EM  VVRV+P+S+YK+ TTRSWDFLGLS+SPS+SSNLLHR             GIW
Sbjct: 94  QKLSEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPSESSNLLHRAKGDDVIIGVIDSGIW 153

Query: 121 PESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEEN 180
           PESE+F D+G+G +PSRWKG CESGE+FNSTNCN+KII ARW+ K F+AD G+EALA+E 
Sbjct: 154 PESESFKDKGLGPIPSRWKGTCESGEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEY 213

Query: 181 SSARDFNGHGTHTASTAAGSLVPNV 193
            S RD +GHGTHTASTAAGS V N+
Sbjct: 214 LSPRDLHGHGTHTASTAAGSFVANI 238

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BLAST of MS012764 vs. NCBI nr
Match: KAA0044826.1 (subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 291.2 bits (744), Expect = 6.5e-75
Identity = 141/205 (68.78%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  H ++K TT+SHH+LL+ +LGSKEKS E+MVYSY+HGFSGFAAKLTKS+A
Sbjct: 34  KVHIVYLGEKPHHDTKFTTDSHHQLLAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR-------------GIW 120
           QKL+EM  VVRV+P+S+YK+ TTRSWDFLGLS+SPS+SSNLLHR             GIW
Sbjct: 94  QKLSEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPSESSNLLHRAKGDDVIIGVIDSGIW 153

Query: 121 PESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEEN 180
           PESE+F D+G+G +PSRWKG CESGE+FNSTNCN+KII ARW+ K F+AD G+EALA+E 
Sbjct: 154 PESESFKDKGLGPIPSRWKGTCESGEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEY 213

Query: 181 SSARDFNGHGTHTASTAAGSLVPNV 193
            S RD +GHGTHTASTAAGS V N+
Sbjct: 214 LSPRDLHGHGTHTASTAAGSFVANI 238

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BLAST of MS012764 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 273.5 bits (698), Expect = 1.4e-69
Identity = 136/206 (66.02%), Postives = 162/206 (78.64%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  +++ KLTT+SHHELL +VLGSKEKS ESMVYSYRHGFSGFAAKLT SQA
Sbjct: 32  KVHIVYLGERPYDDVKLTTDSHHELLESVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQA 91

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           QKLA M  V RV P+S+YKM+TTRSWDFLGLS+SPS+SSNL HR              G 
Sbjct: 92  QKLARMPIVARVFPSSLYKMRTTRSWDFLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGF 151

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE+F D+GMG +PSRWKG C+SGE+FNS++CN+K+I ARW+    IAD G+EA+ ++
Sbjct: 152 WPESESFNDKGMGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFASALIADHGEEAVFKD 211

Query: 181 NSSARDFNGHGTHTASTAAGSLVPNV 193
             SARD  GHGTHTASTA G+ V NV
Sbjct: 212 YLSARDNEGHGTHTASTAGGAFVRNV 237

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BLAST of MS012764 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.0 bits (689), Expect = 1.6e-68
Identity = 135/206 (65.53%), Postives = 160/206 (77.67%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  +++ KLTT+SHHELL +VLGSKEKS ES+VYSYRHGFSGFAAKLT SQA
Sbjct: 34  KVHIVYLGERPYDDVKLTTDSHHELLESVLGSKEKSLESIVYSYRHGFSGFAAKLTNSQA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           QKLA M  V RV P+ +YKM+TTRSWDFLGLS+SPS SSNLLHR              G 
Sbjct: 94  QKLARMPIVARVFPSPLYKMRTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGF 153

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE+F D+GMG +PSRWKG CESGE+FNS++CN+K+I ARW+    +AD G+EA+  +
Sbjct: 154 WPESESFNDKGMGPIPSRWKGICESGEDFNSSHCNKKVIGARWFASALVADHGEEAVFMD 213

Query: 181 NSSARDFNGHGTHTASTAAGSLVPNV 193
             SARD  GHGTHTASTA G+ V NV
Sbjct: 214 YLSARDNQGHGTHTASTAGGAFVRNV 239

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BLAST of MS012764 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-58
Identity = 114/205 (55.61%), Postives = 148/205 (72.20%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ K  TESHH++LS++LGSK+ + ESMVYSYRHGFSGFAAKLTKSQA
Sbjct: 28  KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQS------------SNLLHRGIWP 120
           +K+A+   V+ V+P+S Y++ TTR WD+LG S   S++              ++  G+WP
Sbjct: 88  KKIADSPEVIHVIPDSYYELATTRIWDYLGPSADNSKNLVSDTNMGDQTIIGVIDTGVWP 147

Query: 121 ESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEAL-AEEN 180
           ESE+F D G+G VPS WKG CE GE F STNCNRK+I A+++  GF+A+    A  + + 
Sbjct: 148 ESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKYFINGFLAENQFNATESPDY 207

Query: 181 SSARDFNGHGTHTASTAAGSLVPNV 193
            SARDF+GHGTH AS A GS VPNV
Sbjct: 208 ISARDFDGHGTHVASIAGGSFVPNV 232

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BLAST of MS012764 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 9.9e-58
Identity = 113/210 (53.81%), Postives = 149/210 (70.95%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ +  TESHH +L ++LGSKE + +SMVYSYRHGFSGFAAKLT+SQA
Sbjct: 33  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQA 92

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           +K+A++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH               G+
Sbjct: 93  KKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDTGV 152

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE F D G G VPS WKG CE+GE FNS+NCN+K+I A+++  GF+A+   E+    
Sbjct: 153 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAE--NESFNST 212

Query: 181 NS----SARDFNGHGTHTASTAAGSLVPNV 193
           NS    S RD +GHGTH ++ A GS VPN+
Sbjct: 213 NSLDFISPRDLDGHGTHVSTIAGGSFVPNI 238

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BLAST of MS012764 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.3e-57
Identity = 114/206 (55.34%), Postives = 148/206 (71.84%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ +  +ESHH++LS++LGSK  + ESMVYSYRHGFSGFAAKLT+SQA
Sbjct: 28  KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQA 87

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLS-TSPSQSSN-----------LLHRGIWP 120
           +KLA+   VV V+ +S Y++ TTR+WD+LGLS  +P+   N            +  G+WP
Sbjct: 88  KKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNLLNDTNMGDQVIIGFIDTGVWP 147

Query: 121 ESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEENS 180
           ESE+F D G+G +PS WKG CESGE+F STNCNRK+I A+++  GF+A+       E   
Sbjct: 148 ESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAENEGFNTTESRD 207

Query: 181 --SARDFNGHGTHTASTAAGSLVPNV 193
             SARDF GHGTHTAS A GS VPN+
Sbjct: 208 YISARDFIGHGTHTASIAGGSFVPNI 233

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BLAST of MS012764 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 3.2e-56
Identity = 108/206 (52.43%), Postives = 149/206 (72.33%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE +H + +  TESHH++L+++LGSK+ + +SMVYSYRHGFSGFAAKLTKSQA
Sbjct: 31  KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQA 90

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLST-SPSQSSN-----------LLHRGIWP 120
           +K+A++  VV V+P+  +++ TTR+W++LGLS+ +P    N           ++  G+WP
Sbjct: 91  KKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPKNLLNDTNMGDQVIIGVIDTGVWP 150

Query: 121 ESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEENS 180
           ESE+F D G+G +P +WKG CESGE F ST+CNRK+I A+++  GF+A+       E   
Sbjct: 151 ESESFNDNGVGPIPRKWKGGCESGENFRSTDCNRKLIGAKYFINGFLAENKGFNTTESRD 210

Query: 181 --SARDFNGHGTHTASTAAGSLVPNV 193
             SARDF+GHGTH AS A GS VPNV
Sbjct: 211 YISARDFDGHGTHVASIAGGSFVPNV 236

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BLAST of MS012764 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 2.7e-55
Identity = 110/209 (52.63%), Postives = 148/209 (70.81%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +V++VYLGE +H+  +  TESHH++L ++LGSKE   +S+VYSYRHGFSGFAAKLT+SQA
Sbjct: 28  KVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTESQA 87

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           Q+++E+  VV+V+PN++Y+M TTR+WD+LG+  SP  S +LL +              G+
Sbjct: 88  QQISELPEVVQVIPNTLYEMTTTRTWDYLGV--SPGNSDSLLQKANMGYNVIVGVIDSGV 147

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFN-STNCNRKIISARWYNKGFIADVG--QEAL 180
           WPESE F D+G G +PSRWKG CESGE FN S +CNRK+I A+++  G +A+ G      
Sbjct: 148 WPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNRTQ 207

Query: 181 AEENSSARDFNGHGTHTASTAAGSLVPNV 193
             E  S RDF GHGTH AST  GS +PNV
Sbjct: 208 NPEYLSPRDFAGHGTHVASTIGGSFLPNV 234

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BLAST of MS012764 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.1e-75
Identity = 142/206 (68.93%), Postives = 170/206 (82.52%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  H ++K T +SHH+LLST+LGSKEKS E+MVYSY+HGFSGFAAKLTKSQA
Sbjct: 34  KVHIVYLGEKPHHDTKFTIDSHHQLLSTILGSKEKSMEAMVYSYKHGFSGFAAKLTKSQA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           QKL+EM  VVRV+P+S+YK+ TTRSWDFLGLS+SP +SSNLLHR              GI
Sbjct: 94  QKLSEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPFESSNLLHRAQMGENVIIGVIDTGI 153

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE+F D+G+G +PSRWKG CESGE+FNSTNCN+KII ARW+ KGF+AD+G++ALA+E
Sbjct: 154 WPESESFKDKGVGSIPSRWKGTCESGEQFNSTNCNKKIIGARWFMKGFVADLGRDALAKE 213

Query: 181 NSSARDFNGHGTHTASTAAGSLVPNV 193
             S RD NGHGTHTAS AAGS V N+
Sbjct: 214 YLSPRDLNGHGTHTASIAAGSFVANI 239

[Close]

BLAST of MS012764 vs. ExPASy TrEMBL
Match: A0A5A7TRR2 (Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001180 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.2e-75
Identity = 141/205 (68.78%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  H ++K TT+SHH+LL+ +LGSKEKS E+MVYSY+HGFSGFAAKLTKS+A
Sbjct: 34  KVHIVYLGEKPHHDTKFTTDSHHQLLAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR-------------GIW 120
           QKL+EM  VVRV+P+S+YK+ TTRSWDFLGLS+SPS+SSNLLHR             GIW
Sbjct: 94  QKLSEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPSESSNLLHRAKGDDVIIGVIDSGIW 153

Query: 121 PESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEEN 180
           PESE+F D+G+G +PSRWKG CESGE+FNSTNCN+KII ARW+ K F+AD G+EALA+E 
Sbjct: 154 PESESFKDKGLGPIPSRWKGTCESGEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEY 213

Query: 181 SSARDFNGHGTHTASTAAGSLVPNV 193
            S RD +GHGTHTASTAAGS V N+
Sbjct: 214 LSPRDLHGHGTHTASTAAGSFVANI 238

[Close]

BLAST of MS012764 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 3.2e-75
Identity = 141/205 (68.78%), Postives = 170/205 (82.93%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  H ++K TT+SHH+LL+ +LGSKEKS E+MVYSY+HGFSGFAAKLTKS+A
Sbjct: 34  KVHIVYLGEKPHHDTKFTTDSHHQLLAAILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEA 93

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR-------------GIW 120
           QKL+EM  VVRV+P+S+YK+ TTRSWDFLGLS+SPS+SSNLLHR             GIW
Sbjct: 94  QKLSEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPSESSNLLHRAKGDDVIIGVIDSGIW 153

Query: 121 PESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEEN 180
           PESE+F D+G+G +PSRWKG CESGE+FNSTNCN+KII ARW+ K F+AD G+EALA+E 
Sbjct: 154 PESESFKDKGLGPIPSRWKGTCESGEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEY 213

Query: 181 SSARDFNGHGTHTASTAAGSLVPNV 193
            S RD +GHGTHTASTAAGS V N+
Sbjct: 214 LSPRDLHGHGTHTASTAAGSFVANI 238

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BLAST of MS012764 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 6.8e-70
Identity = 136/206 (66.02%), Postives = 162/206 (78.64%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE  +++ KLTT+SHHELL +VLGSKEKS ESMVYSYRHGFSGFAAKLT SQA
Sbjct: 32  KVHIVYLGERPYDDVKLTTDSHHELLESVLGSKEKSLESMVYSYRHGFSGFAAKLTNSQA 91

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           QKLA M  V RV P+S+YKM+TTRSWDFLGLS+SPS+SSNL HR              G 
Sbjct: 92  QKLARMPIVARVFPSSLYKMRTTRSWDFLGLSSSPSESSNLFHRAKMGDNVIIGVIDTGF 151

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE+F D+GMG +PSRWKG C+SGE+FNS++CN+K+I ARW+    IAD G+EA+ ++
Sbjct: 152 WPESESFNDKGMGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFASALIADHGEEAVFKD 211

Query: 181 NSSARDFNGHGTHTASTAAGSLVPNV 193
             SARD  GHGTHTASTA G+ V NV
Sbjct: 212 YLSARDNEGHGTHTASTAGGAFVRNV 237

[Close]

BLAST of MS012764 vs. ExPASy TrEMBL
Match: A0A6J1EUE7 (subtilisin-like protease SBT3.9 isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 7.6e-69
Identity = 134/206 (65.05%), Postives = 162/206 (78.64%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +V+I YLGE  +E+ KLTT+SHHELL +V+GSKEKS ESM+YSYRHGFSGFAAKLT SQA
Sbjct: 14  KVYIAYLGERPYEDVKLTTDSHHELLESVMGSKEKSLESMMYSYRHGFSGFAAKLTNSQA 73

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           QKLA M  V RV P+S+YKM+TTRSWDFLGLS+SPS SSNLLHR              G 
Sbjct: 74  QKLARMPIVARVFPSSLYKMRTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDTGF 133

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE+F D+GMG +PSRWKG C+SGE+FNS++CN+K+I ARW+ +  IAD G+EA+ ++
Sbjct: 134 WPESESFNDKGMGPIPSRWKGICQSGEDFNSSHCNKKVIGARWFARALIADHGEEAVFKD 193

Query: 181 NSSARDFNGHGTHTASTAAGSLVPNV 193
             SARD  GHGTHTASTA G+ V NV
Sbjct: 194 YLSARDNKGHGTHTASTAGGAFVRNV 219

[Close]

BLAST of MS012764 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-59
Identity = 114/205 (55.61%), Postives = 148/205 (72.20%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ K  TESHH++LS++LGSK+ + ESMVYSYRHGFSGFAAKLTKSQA
Sbjct: 28  KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQS------------SNLLHRGIWP 120
           +K+A+   V+ V+P+S Y++ TTR WD+LG S   S++              ++  G+WP
Sbjct: 88  KKIADSPEVIHVIPDSYYELATTRIWDYLGPSADNSKNLVSDTNMGDQTIIGVIDTGVWP 147

Query: 121 ESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEAL-AEEN 180
           ESE+F D G+G VPS WKG CE GE F STNCNRK+I A+++  GF+A+    A  + + 
Sbjct: 148 ESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKYFINGFLAENQFNATESPDY 207

Query: 181 SSARDFNGHGTHTASTAAGSLVPNV 193
            SARDF+GHGTH AS A GS VPNV
Sbjct: 208 ISARDFDGHGTHVASIAGGSFVPNV 232

[Close]

BLAST of MS012764 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 224.6 bits (571), Expect = 7.0e-59
Identity = 113/210 (53.81%), Postives = 149/210 (70.95%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ +  TESHH +L ++LGSKE + +SMVYSYRHGFSGFAAKLT+SQA
Sbjct: 33  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQA 92

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           +K+A++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH               G+
Sbjct: 93  KKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDTGV 152

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE F D G G VPS WKG CE+GE FNS+NCN+K+I A+++  GF+A+   E+    
Sbjct: 153 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAE--NESFNST 212

Query: 181 NS----SARDFNGHGTHTASTAAGSLVPNV 193
           NS    S RD +GHGTH ++ A GS VPN+
Sbjct: 213 NSLDFISPRDLDGHGTHVSTIAGGSFVPNI 238

[Close]

BLAST of MS012764 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 224.6 bits (571), Expect = 7.0e-59
Identity = 113/210 (53.81%), Postives = 149/210 (70.95%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ +  TESHH +L ++LGSKE + +SMVYSYRHGFSGFAAKLT+SQA
Sbjct: 49  KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQA 108

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLSTSPSQSSNLLHR--------------GI 120
           +K+A++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH               G+
Sbjct: 109 KKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDTGV 168

Query: 121 WPESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEE 180
           WPESE F D G G VPS WKG CE+GE FNS+NCN+K+I A+++  GF+A+   E+    
Sbjct: 169 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAE--NESFNST 228

Query: 181 NS----SARDFNGHGTHTASTAAGSLVPNV 193
           NS    S RD +GHGTH ++ A GS VPN+
Sbjct: 229 NSLDFISPRDLDGHGTHVSTIAGGSFVPNI 254

[Close]

BLAST of MS012764 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 224.2 bits (570), Expect = 9.2e-59
Identity = 114/206 (55.34%), Postives = 148/206 (71.84%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE QH++ +  +ESHH++LS++LGSK  + ESMVYSYRHGFSGFAAKLT+SQA
Sbjct: 28  KVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQA 87

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLS-TSPSQSSN-----------LLHRGIWP 120
           +KLA+   VV V+ +S Y++ TTR+WD+LGLS  +P+   N            +  G+WP
Sbjct: 88  KKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNLLNDTNMGDQVIIGFIDTGVWP 147

Query: 121 ESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEENS 180
           ESE+F D G+G +PS WKG CESGE+F STNCNRK+I A+++  GF+A+       E   
Sbjct: 148 ESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAENEGFNTTESRD 207

Query: 181 --SARDFNGHGTHTASTAAGSLVPNV 193
             SARDF GHGTHTAS A GS VPN+
Sbjct: 208 YISARDFIGHGTHTASIAGGSFVPNI 233

[Close]

BLAST of MS012764 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 219.5 bits (558), Expect = 2.3e-57
Identity = 108/206 (52.43%), Postives = 149/206 (72.33%), Query Frame = 0


Query: 1   QVHIVYLGETQHEESKLTTESHHELLSTVLGSKEKSWESMVYSYRHGFSGFAAKLTKSQA 60
           +VHIVYLGE +H + +  TESHH++L+++LGSK+ + +SMVYSYRHGFSGFAAKLTKSQA
Sbjct: 31  KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQA 90

Query: 61  QKLAEMVGVVRVLPNSIYKMQTTRSWDFLGLST-SPSQSSN-----------LLHRGIWP 120
           +K+A++  VV V+P+  +++ TTR+W++LGLS+ +P    N           ++  G+WP
Sbjct: 91  KKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPKNLLNDTNMGDQVIIGVIDTGVWP 150

Query: 121 ESEAFGDEGMGRVPSRWKGKCESGEEFNSTNCNRKIISARWYNKGFIADVGQEALAEENS 180
           ESE+F D G+G +P +WKG CESGE F ST+CNRK+I A+++  GF+A+       E   
Sbjct: 151 ESESFNDNGVGPIPRKWKGGCESGENFRSTDCNRKLIGAKYFINGFLAENKGFNTTESRD 210

Query: 181 --SARDFNGHGTHTASTAAGSLVPNV 193
             SARDF+GHGTH AS A GS VPNV
Sbjct: 211 YISARDFDGHGTHVASIAGGSFVPNV 236

The following BLAST results are available for this feature:

BLAST of MS012764 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146562.1	2.2e-75	68.93	subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical prote...	[more]
XP_008452040.1	6.5e-75	68.78	PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo]	[more]
KAA0044826.1	6.5e-75	68.78	subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_022984814.1	1.4e-69	66.02	subtilisin-like protease SBT3.9 [Cucurbita maxima]	[more]
XP_023552781.1	1.6e-68	65.53	subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS012764 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HPF1	1.5e-58	55.61	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q8L7I2	9.9e-58	53.81	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
Q9MAP7	1.3e-57	55.34	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]
Q9MAP5	3.2e-56	52.43	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
Q9ZSB0	2.7e-55	52.63	Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S...	[more]

BLAST of MS012764 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWS6	1.1e-75	68.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1	[more]
A0A5A7TRR2	3.2e-75	68.78	Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BU23	3.2e-75	68.78	subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034931...	[more]
A0A6J1JBL4	6.8e-70	66.02	subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3...	[more]
A0A6J1EUE7	7.6e-69	65.05	subtilisin-like protease SBT3.9 isoform X7 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of MS012764 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32950.1	1.1e-59	55.61	Subtilase family protein	[more]
AT4G10550.1	7.0e-59	53.81	Subtilase family protein	[more]
AT4G10550.3	7.0e-59	53.81	Subtilase family protein	[more]
AT1G32940.1	9.2e-59	55.34	Subtilase family protein	[more]
AT1G32960.1	2.3e-57	52.43	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 2..80 e-value: 8.5E-16 score: 58.4
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 1..81 e-value: 9.6E-25 score: 88.9
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 104..192 e-value: 8.2E-29 score: 102.7
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 15..187
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..192
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 171..192
None	No IPR available	PANTHER	PTHR10795:SF671	BNAC01G30950D PROTEIN	coord: 2..192
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 2..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012764.1	MS012764.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity