MS012716 (gene) Bitter gourd (TR) v1

Overview


Name	MS012716
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin-conjugating enzyme family protein
Location	scaffold63: 2917654 .. 2920237 (+)
RNA-Seq Expression	MS012716
Synteny	MS012716

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGCTCTGAGTAAGATTGCTTGCAATCGGCTCCAGAAAGAACTTGTTGAGTGGCAGGTCAACCCTCCCGCTGGTTTCAAACACAAGGTCACCGATAATCTTCAGAGGTATTTGTTCTTCCTTTTCCTCGTAGGGTTTTCAATTCGTCTCTGCCGTTCCAACTCGGGAAAGCAAAATAAGGCTAGAATTTGATGTAATTGCGTCGAATTGGTGTTGTTGGGGCTTGGTTTAGGTTTGATTGTTCTGAAACGAATTTTACTCTCTTCTGCTTCTAGTACGAATTTTATATTTATTTCTGGGGGTTTTTGTTGGAAGGTGGGTGATTGAAGTCAACGGAGCTCCAGGTACTCTCTATGCAAATGAAACCTACCAGCTTCAGGTGGACTTTCCAGAGCATTACCCAATGGAAGCCCCACAGGTCTAACATAATCATGGAATTCTGTTCTGAGATCACTTCTATTTCATAATCCTTTAGCGGCTATATTTATTGATTGTTTTGTATTTCATTTTGTCGTGCCCGTTTTACAGGTCATATTTCTGCATCCAGCCCCTCTGCACCCTCATATCTATAGCAATGGTCACATTTGTCTAGGTTGGTATATATTGTTTTGCTTCACTAGACCACTCTATTTAAGAGATTTCAGTTCTGCAGGAATATCAAGTTTATAATCTATTGCTTTCTTCACTCTGTAGTATCTGTTTCGATTTTTTCCCCCCTATTTTTATCTGCCTAGGTTTGAGCTTAACACTATCTATAAGGAGTTTTGTTTATAATTTTTGTTTCAGAGGGTAGATTTCAGAGGTTTTAAGTAATTATGTTTAGTTGAATTTCACCCTAGTAAATATCTCAAGTGTAGACATTAATAGATTTTCCTACCATATTGTGTGATGCTACCTGTAGGCCTACTTAGCAGTGACTCGTCATGAAAAACCTAAATGAATTGATGTTACAAAAAGTTTTGATATATACCTTATCCTCTTAGTTCAACAGAAAAAAGATCACTTTATGTTGGATGGGACAACGTGTGGCTTACATCTATTTTTCCATTTACAACTTTTGAAATACACAAATACTGAAGTATAGACTAGTTTACAGAGAAAGTACTGCTTGATGTCGGATTTCATCCCTTTTTTGGTAATAGCTAGTTTGTATCGGATGAAAAGACCTATGCTGAGAATTTATTTTAAGATCAGTGTTTATTGCATAAGGGTATTTGCTACGGTGTTTGTTGATTTGGTTTGTTATGGAATCTTTGAAATCAGTTGGTTTTATATTTAAAATCAATAAGAACAGAACCAAACTGTTCATCACTAAAAACCAATCCAATCCATCAATATATTGATTTGTATCATCTGTAACTTCTTTCATTACGTTTGCCTTTTCAAATGAAATATAAATATGATAAAATTCACTTAAATCTGACAGCCTACCTGCAATATTAGCAAAACTAATTCCAATTAATTTAAAAGTCTAAGCTAACAAATAAAGATAACGTAAAGGAATTCACAAACATTTCGGATTTAAAGTCTTTGAAACTTGACTTTAGACTTTAGAAGAAATCATTAATCATGTATTATGTAAAATCATTTTAGGATTATTTATTTAGTTATTTTAGCTATCTAAGTTCTATTCATCTGTTTGGTTTAGACAGAAATATGTTGAACTGAATGAGACTATCAGTGATTGGTTTGTTTGGTTTAGACATAAAGAAAGAGAAATCTAAACCAAACCAATTTAGTTTGGATCGTTGGTTTGGTTTTGGACACCTCTATTATTTGCAGTGGATGTAGTTTCAAGGTAAAGTTGGGAACATGCTTTCTGCAACTTGCCAGTCTCTCGGATGCTCTTTATATGTGTAACTGAGATGATGCGTTTTCCTTGAATTTGATATGATGGTTTAAAAGTTACATCTGTTACTTTTTCCTCTCATACATGTTTCTAACCAGCGTTTCACTGTTTGTTACTAATACATGTTGGAGTTTTGAATTCTATCCTACTTGTGTATAACTGTGATATTGGTTAGCTTTGTCTGAGAGCCTTTGTCAAGCACCACCATAGCATGACTGATCATTGTTATTTTCCTTCACTTTGAAACTGTGTAGATATTTTGTATGATTCATGGTCGCCTGCGATGACTGTAAGTTCTATCTGTATCAGCATTCTCTCCATGCTGTCAAGCTCCACGGCAAAGGTCTGATACATTCCTTTACACTTTGATTCTATCTAATACGTTAGTTGTTTTTCTTGGACGTGTTGAAAATAGGAAAATTCTTGCATTTTTGAAGTTAAGATGTAAGAATGGTCGTGTGTTCTGCATAGGATGATATGCTTTGAAATTCAAGATCTACTGTTATTATTTGCTCTAATACCTAAAGATAGGGAAGTTTGGGGGGTGGGGGGCTGTGAGATAGTACTGTCTAATTCAGATTCAGGTGTTAAGTGTCAACCATAACCCATTCCCTAATACTCTGATTTTTTTCTCTGTTGCAGCAACGCCCTGACGATAACGACCGTTATGTAAAGAATTGTAGGAACGGAAGATCTCCAAAAGAGACGAGGTGGTGGTTCCATGACGATAAGGTG

mRNA sequence

CAGGCTCTGAGTAAGATTGCTTGCAATCGGCTCCAGAAAGAACTTGTTGAGTGGCAGGTCAACCCTCCCGCTGGTTTCAAACACAAGGTCACCGATAATCTTCAGAGGTGGGTGATTGAAGTCAACGGAGCTCCAGGTACTCTCTATGCAAATGAAACCTACCAGCTTCAGGTGGACTTTCCAGAGCATTACCCAATGGAAGCCCCACAGGTCATATTTCTGCATCCAGCCCCTCTGCACCCTCATATCTATAGCAATGGTCACATTTGTCTAGATATTTTGTATGATTCATGGTCGCCTGCGATGACTGTAAGTTCTATCTGTATCAGCATTCTCTCCATGCTGTCAAGCTCCACGGCAAAGCAACGCCCTGACGATAACGACCGTTATGTAAAGAATTGTAGGAACGGAAGATCTCCAAAAGAGACGAGGTGGTGGTTCCATGACGATAAGGTG

Coding sequence (CDS)

CAGGCTCTGAGTAAGATTGCTTGCAATCGGCTCCAGAAAGAACTTGTTGAGTGGCAGGTCAACCCTCCCGCTGGTTTCAAACACAAGGTCACCGATAATCTTCAGAGGTGGGTGATTGAAGTCAACGGAGCTCCAGGTACTCTCTATGCAAATGAAACCTACCAGCTTCAGGTGGACTTTCCAGAGCATTACCCAATGGAAGCCCCACAGGTCATATTTCTGCATCCAGCCCCTCTGCACCCTCATATCTATAGCAATGGTCACATTTGTCTAGATATTTTGTATGATTCATGGTCGCCTGCGATGACTGTAAGTTCTATCTGTATCAGCATTCTCTCCATGCTGTCAAGCTCCACGGCAAAGCAACGCCCTGACGATAACGACCGTTATGTAAAGAATTGTAGGAACGGAAGATCTCCAAAAGAGACGAGGTGGTGGTTCCATGACGATAAGGTG

Protein sequence

QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDFPEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTAKQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV

Homology

[Close]

BLAST of MS012716 vs. NCBI nr
Match: XP_022136917.1 (probable ubiquitin-conjugating enzyme E2 16 [Momordica charantia])

HSP 1 Score: 324.7 bits (831), Expect = 4.2e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. NCBI nr
Match: XP_004146492.2 (probable ubiquitin-conjugating enzyme E2 16 [Cucumis sativus] >XP_008452114.1 PREDICTED: probable ubiquitin-conjugating enzyme E2 16 [Cucumis melo] >KAA0044779.1 putative ubiquitin-conjugating enzyme E2 16 [Cucumis melo var. makuwa] >KGN53285.1 hypothetical protein Csa_014866 [Cucumis sativus])

HSP 1 Score: 324.7 bits (831), Expect = 4.2e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. NCBI nr
Match: XP_022942312.1 (probable ubiquitin-conjugating enzyme E2 16 [Cucurbita moschata] >XP_022983104.1 probable ubiquitin-conjugating enzyme E2 16 [Cucurbita maxima] >XP_023513778.1 probable ubiquitin-conjugating enzyme E2 16 [Cucurbita pepo subsp. pepo] >KAG6600596.1 putative ubiquitin-conjugating enzyme E2 16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031233.1 putative ubiquitin-conjugating enzyme E2 16 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 324.7 bits (831), Expect = 4.2e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. NCBI nr
Match: XP_038877729.1 (probable ubiquitin-conjugating enzyme E2 16 [Benincasa hispida])

HSP 1 Score: 322.4 bits (825), Expect = 2.1e-84
Identity = 149/152 (98.03%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKEL+EWQ+NPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELLEWQLNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. NCBI nr
Match: XP_022929509.1 (probable ubiquitin-conjugating enzyme E2 18 [Cucurbita moschata] >XP_022985349.1 probable ubiquitin-conjugating enzyme E2 18 [Cucurbita maxima] >XP_023553462.1 probable ubiquitin-conjugating enzyme E2 18 [Cucurbita pepo subsp. pepo] >KAG6577233.1 putative ubiquitin-conjugating enzyme E2 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 321.2 bits (822), Expect = 4.7e-84
Identity = 149/152 (98.03%), Postives = 151/152 (99.34%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKH+VTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHRVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSS A
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSPA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy Swiss-Prot
Match: Q9FWT2 (Probable ubiquitin-conjugating enzyme E2 16 OS=Arabidopsis thaliana OX=3702 GN=UBC16 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 5.9e-82
Identity = 140/152 (92.11%), Postives = 146/152 (96.05%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           + LSKIA NRLQKELVEWQ+NPP GFKHKVTDNLQRW+IEV GAPGTLYAN+TYQLQVDF
Sbjct: 10  KTLSKIATNRLQKELVEWQMNPPTGFKHKVTDNLQRWIIEVIGAPGTLYANDTYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPME+PQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSST 
Sbjct: 70  PEHYPMESPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTE 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRP DNDRYVKNC+NGRSPKETRWWFHDDKV
Sbjct: 130 KQRPTDNDRYVKNCKNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy Swiss-Prot
Match: Q9FMM0 (Probable ubiquitin-conjugating enzyme E2 18 OS=Arabidopsis thaliana OX=3702 GN=UBC18 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 5.6e-80
Identity = 136/152 (89.47%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKEL EWQVNPP GFKH+VTDNLQ+WVIEV GAPGTLYANETY LQV+F
Sbjct: 10  KALSKIACNRLQKELSEWQVNPPTGFKHRVTDNLQKWVIEVTGAPGTLYANETYNLQVEF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           P+HYPMEAPQVIF+ PAPLHPHIYSNGHICLDILYDSWSPAMTVSS+CISILSMLSSS  
Sbjct: 70  PQHYPMEAPQVIFVPPAPLHPHIYSNGHICLDILYDSWSPAMTVSSVCISILSMLSSSPE 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRP DNDRYVKNC+NGRSPKETRWWFHDDKV
Sbjct: 130 KQRPTDNDRYVKNCKNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy Swiss-Prot
Match: P42743 (Ubiquitin-conjugating enzyme 15 OS=Arabidopsis thaliana OX=3702 GN=UBC15 PE=2 SV=2)

HSP 1 Score: 295.4 bits (755), Expect = 3.6e-79
Identity = 133/152 (87.50%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKEL EWQ+NPP GF+HKVTDNLQ+W I+V GAPGTLYANETYQLQV+F
Sbjct: 10  KALSKIACNRLQKELSEWQLNPPTGFRHKVTDNLQKWTIDVTGAPGTLYANETYQLQVEF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQV+F+ PAP HPHIYSNGHICLDILYDSWSPAMTV+S+CISILSMLSSS A
Sbjct: 70  PEHYPMEAPQVVFVSPAPSHPHIYSNGHICLDILYDSWSPAMTVNSVCISILSMLSSSPA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRP DNDRYVKNC+NGRSPKETRWWFHDDKV
Sbjct: 130 KQRPADNDRYVKNCKNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy Swiss-Prot
Match: O23239 (Probable ubiquitin-conjugating enzyme E2 17 OS=Arabidopsis thaliana OX=3702 GN=UBC17 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 2.7e-74
Identity = 124/152 (81.58%), Postives = 141/152 (92.76%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           + L+KIA NRLQKE +EWQ NPP+GFKH+V+DNLQRW+IEV+G PGTLYANETYQLQV+F
Sbjct: 10  KGLTKIATNRLQKEFMEWQTNPPSGFKHRVSDNLQRWIIEVHGVPGTLYANETYQLQVEF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIF HPAPLHPHIYSNGHICLD+LYDSWSPAM +SSIC+SILSMLSSS+ 
Sbjct: 70  PEHYPMEAPQVIFQHPAPLHPHIYSNGHICLDVLYDSWSPAMRLSSICLSILSMLSSSSV 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQ+P DND Y+KNC++GRSPKETRW FHDDKV
Sbjct: 130 KQKPKDNDHYLKNCKHGRSPKETRWRFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy Swiss-Prot
Match: Q55EY8 (Probable ubiquitin-conjugating enzyme E2 W OS=Dictyostelium discoideum OX=44689 GN=ube2w PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 81/143 (56.64%), Postives = 103/143 (72.03%), Query Frame = 0


Query: 10  RLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDFPEHYPMEAP 69
           RLQKEL++ + NPP        DNL +WVI V+G  G++Y  E ++LQ  F   YP+++P
Sbjct: 8   RLQKELLDLKTNPPPCISITEGDNLDKWVIAVDGTEGSIYQGEHFKLQFKFSSGYPLDSP 67

Query: 70  QVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTAKQRPDDNDR 129
           +VIF+   P+HPHIYSNGHICL ILYD+WSPA+TVSS+C+SILSMLS  T K RP D+ +
Sbjct: 68  EVIFIGTPPIHPHIYSNGHICLSILYDNWSPALTVSSVCLSILSMLSGCTEKIRPTDDSK 127

Query: 130 YVKNCRNGRSPKETRWWFHDDKV 153
           YV    N +SPKE RW FHDD V
Sbjct: 128 YVSRVLN-KSPKEVRWMFHDDTV 149

[Close]

BLAST of MS012716 vs. ExPASy TrEMBL
Match: A0A6J1J6C9 (probable ubiquitin-conjugating enzyme E2 16 OS=Cucurbita maxima OX=3661 GN=LOC111481748 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.0e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy TrEMBL
Match: A0A5A7TU62 (Putative ubiquitin-conjugating enzyme E2 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00690 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.0e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy TrEMBL
Match: A0A0A0KUH0 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G045000 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.0e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy TrEMBL
Match: A0A6J1C6S5 (probable ubiquitin-conjugating enzyme E2 16 OS=Momordica charantia OX=3673 GN=LOC111008493 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.0e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. ExPASy TrEMBL
Match: A0A6J1FPW6 (probable ubiquitin-conjugating enzyme E2 16 OS=Cucurbita moschata OX=3662 GN=LOC111447396 PE=3 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 2.0e-85
Identity = 151/152 (99.34%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF
Sbjct: 10  KALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA
Sbjct: 70  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV
Sbjct: 130 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. TAIR 10
Match: AT1G75440.1 (ubiquitin-conjugating enzyme 16 )

HSP 1 Score: 304.7 bits (779), Expect = 4.2e-83
Identity = 140/152 (92.11%), Postives = 146/152 (96.05%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           + LSKIA NRLQKELVEWQ+NPP GFKHKVTDNLQRW+IEV GAPGTLYAN+TYQLQVDF
Sbjct: 10  KTLSKIATNRLQKELVEWQMNPPTGFKHKVTDNLQRWIIEVIGAPGTLYANDTYQLQVDF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPME+PQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSST 
Sbjct: 70  PEHYPMESPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTE 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRP DNDRYVKNC+NGRSPKETRWWFHDDKV
Sbjct: 130 KQRPTDNDRYVKNCKNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. TAIR 10
Match: AT5G42990.1 (ubiquitin-conjugating enzyme 18 )

HSP 1 Score: 298.1 bits (762), Expect = 3.9e-81
Identity = 136/152 (89.47%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKEL EWQVNPP GFKH+VTDNLQ+WVIEV GAPGTLYANETY LQV+F
Sbjct: 10  KALSKIACNRLQKELSEWQVNPPTGFKHRVTDNLQKWVIEVTGAPGTLYANETYNLQVEF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           P+HYPMEAPQVIF+ PAPLHPHIYSNGHICLDILYDSWSPAMTVSS+CISILSMLSSS  
Sbjct: 70  PQHYPMEAPQVIFVPPAPLHPHIYSNGHICLDILYDSWSPAMTVSSVCISILSMLSSSPE 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRP DNDRYVKNC+NGRSPKETRWWFHDDKV
Sbjct: 130 KQRPTDNDRYVKNCKNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. TAIR 10
Match: AT1G45050.1 (Ubiquitin-conjugating enzyme family protein )

HSP 1 Score: 295.4 bits (755), Expect = 2.6e-80
Identity = 133/152 (87.50%), Postives = 144/152 (94.74%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           +ALSKIACNRLQKEL EWQ+NPP GF+HKVTDNLQ+W I+V GAPGTLYANETYQLQV+F
Sbjct: 10  KALSKIACNRLQKELSEWQLNPPTGFRHKVTDNLQKWTIDVTGAPGTLYANETYQLQVEF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQV+F+ PAP HPHIYSNGHICLDILYDSWSPAMTV+S+CISILSMLSSS A
Sbjct: 70  PEHYPMEAPQVVFVSPAPSHPHIYSNGHICLDILYDSWSPAMTVNSVCISILSMLSSSPA 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQRP DNDRYVKNC+NGRSPKETRWWFHDDKV
Sbjct: 130 KQRPADNDRYVKNCKNGRSPKETRWWFHDDKV 161

[Close]

BLAST of MS012716 vs. TAIR 10
Match: AT4G36410.1 (ubiquitin-conjugating enzyme 17 )

HSP 1 Score: 279.3 bits (713), Expect = 1.9e-75
Identity = 124/152 (81.58%), Postives = 141/152 (92.76%), Query Frame = 0


Query: 1   QALSKIACNRLQKELVEWQVNPPAGFKHKVTDNLQRWVIEVNGAPGTLYANETYQLQVDF 60
           + L+KIA NRLQKE +EWQ NPP+GFKH+V+DNLQRW+IEV+G PGTLYANETYQLQV+F
Sbjct: 10  KGLTKIATNRLQKEFMEWQTNPPSGFKHRVSDNLQRWIIEVHGVPGTLYANETYQLQVEF 69

Query: 61  PEHYPMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTA 120
           PEHYPMEAPQVIF HPAPLHPHIYSNGHICLD+LYDSWSPAM +SSIC+SILSMLSSS+ 
Sbjct: 70  PEHYPMEAPQVIFQHPAPLHPHIYSNGHICLDVLYDSWSPAMRLSSICLSILSMLSSSSV 129

Query: 121 KQRPDDNDRYVKNCRNGRSPKETRWWFHDDKV 153
           KQ+P DND Y+KNC++GRSPKETRW FHDDKV
Sbjct: 130 KQKPKDNDHYLKNCKHGRSPKETRWRFHDDKV 161

[Close]

BLAST of MS012716 vs. TAIR 10
Match: AT2G16740.1 (ubiquitin-conjugating enzyme 29 )

HSP 1 Score: 84.7 bits (208), Expect = 6.9e-17
Identity = 46/122 (37.70%), Postives = 69/122 (56.56%), Query Frame = 0


Query: 6   IACNRLQKELVEWQVNPPAGFKHKVT-DNLQRWVIEVNGAPGTLYANETYQLQVDFPEHY 65
           +A  R+ KEL E Q +PP       T +++  W   + G   + Y+   + + + FP  Y
Sbjct: 1   MATRRILKELKELQRDPPVSCSAGPTGEDMFHWQATIMGPNESPYSGGVFLVNIHFPPDY 60

Query: 66  PMEAPQVIFLHPAPLHPHIYSNGHICLDILYDSWSPAMTVSSICISILSMLSSSTAKQRP 125
           P + P+V+F      HP+I SNG+ICLDIL D WSPA+T+S + +SI S+L+       P
Sbjct: 61  PFKPPKVVF-RTKVFHPNINSNGNICLDILKDQWSPALTISKVLLSICSLLTD----PNP 117

Query: 126 DD 127
           DD
Sbjct: 121 DD 117

The following BLAST results are available for this feature:

BLAST of MS012716 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136917.1	4.2e-85	99.34	probable ubiquitin-conjugating enzyme E2 16 [Momordica charantia]	[more]
XP_004146492.2	4.2e-85	99.34	probable ubiquitin-conjugating enzyme E2 16 [Cucumis sativus] >XP_008452114.1 PR...	[more]
XP_022942312.1	4.2e-85	99.34	probable ubiquitin-conjugating enzyme E2 16 [Cucurbita moschata] >XP_022983104.1...	[more]
XP_038877729.1	2.1e-84	98.03	probable ubiquitin-conjugating enzyme E2 16 [Benincasa hispida]	[more]
XP_022929509.1	4.7e-84	98.03	probable ubiquitin-conjugating enzyme E2 18 [Cucurbita moschata] >XP_022985349.1...	[more]

BLAST of MS012716 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FWT2	5.9e-82	92.11	Probable ubiquitin-conjugating enzyme E2 16 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q9FMM0	5.6e-80	89.47	Probable ubiquitin-conjugating enzyme E2 18 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
P42743	3.6e-79	87.50	Ubiquitin-conjugating enzyme 15 OS=Arabidopsis thaliana OX=3702 GN=UBC15 PE=2 SV...	[more]
O23239	2.7e-74	81.58	Probable ubiquitin-conjugating enzyme E2 17 OS=Arabidopsis thaliana OX=3702 GN=U...	[more]
Q55EY8	1.2e-42	56.64	Probable ubiquitin-conjugating enzyme E2 W OS=Dictyostelium discoideum OX=44689 ...	[more]

BLAST of MS012716 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J6C9	2.0e-85	99.34	probable ubiquitin-conjugating enzyme E2 16 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A5A7TU62	2.0e-85	99.34	Putative ubiquitin-conjugating enzyme E2 16 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0KUH0	2.0e-85	99.34	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G045000 PE...	[more]
A0A6J1C6S5	2.0e-85	99.34	probable ubiquitin-conjugating enzyme E2 16 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1FPW6	2.0e-85	99.34	probable ubiquitin-conjugating enzyme E2 16 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of MS012716 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75440.1	4.2e-83	92.11	ubiquitin-conjugating enzyme 16	[more]
AT5G42990.1	3.9e-81	89.47	ubiquitin-conjugating enzyme 18	[more]
AT1G45050.1	2.6e-80	87.50	Ubiquitin-conjugating enzyme family protein	[more]
AT4G36410.1	1.9e-75	81.58	ubiquitin-conjugating enzyme 17	[more]
AT2G16740.1	6.9e-17	37.70	ubiquitin-conjugating enzyme 29	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 9..152 e-value: 3.8E-47 score: 172.6
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 5..152
None	No IPR available	PANTHER	PTHR24068:SF302	UBIQUITIN-CONJUGATING ENZYME E2 16-RELATED	coord: 5..152
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 10..145 e-value: 2.3E-38 score: 131.0
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 6..152 score: 40.575031
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 8..108 e-value: 5.89794E-37 score: 121.926
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 6..152 e-value: 6.3E-48 score: 164.2
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 4..133
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 79..94

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012716.1	MS012716.1	mRNA