Homology
BLAST of MS012710 vs. NCBI nr
Match:
KAG7031236.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 631.3 bits (1627), Expect = 5.1e-177
Identity = 293/340 (86.18%), Postives = 321/340 (94.41%), Query Frame = 0
Query: 27 EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
+VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK AGGFRSVQKAI S+P+VNR
Sbjct: 35 KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNR 94
Query: 87 CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154
Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214
Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 274
Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 334
Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
GGRVSWSRELTESEA+PF+SL FI A EWL PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372
BLAST of MS012710 vs. NCBI nr
Match:
KAG6600598.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 631.3 bits (1627), Expect = 5.1e-177
Identity = 293/340 (86.18%), Postives = 321/340 (94.41%), Query Frame = 0
Query: 27 EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
+VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK AGGFRSVQKAI S+P+VNR
Sbjct: 72 KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNR 131
Query: 87 CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 132 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 191
Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 192 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 251
Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 252 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 311
Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 312 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 371
Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
GGRVSWSRELTESEA+PF+SL FI A EWL PK THLAI
Sbjct: 372 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 409
BLAST of MS012710 vs. NCBI nr
Match:
XP_023527790.1 (probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 629.0 bits (1621), Expect = 2.5e-176
Identity = 292/340 (85.88%), Postives = 320/340 (94.12%), Query Frame = 0
Query: 27 EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
+VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK AGGFRSVQKAI S+P++NR
Sbjct: 35 KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 94
Query: 87 CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154
Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
FAVNSPFFVARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFVARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214
Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+ CKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKV 274
Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 334
Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
GGRVSWSRELTESEA+PF+SL FI A EWL PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372
BLAST of MS012710 vs. NCBI nr
Match:
XP_023527781.1 (probable pectinesterase 53 isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 629.0 bits (1621), Expect = 2.5e-176
Identity = 292/340 (85.88%), Postives = 320/340 (94.12%), Query Frame = 0
Query: 27 EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
+VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK AGGFRSVQKAI S+P++NR
Sbjct: 62 KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 121
Query: 87 CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 122 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 181
Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
FAVNSPFFVARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 182 FAVNSPFFVARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 241
Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+ CKV
Sbjct: 242 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKV 301
Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 302 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 361
Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
GGRVSWSRELTESEA+PF+SL FI A EWL PK THLAI
Sbjct: 362 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 399
BLAST of MS012710 vs. NCBI nr
Match:
XP_022942305.1 (probable pectinesterase 53 [Cucurbita moschata])
HSP 1 Score: 628.2 bits (1619), Expect = 4.3e-176
Identity = 291/340 (85.59%), Postives = 320/340 (94.12%), Query Frame = 0
Query: 27 EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
+VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK AGGFRSVQKAI S+P++NR
Sbjct: 35 KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 94
Query: 87 CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154
Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214
Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 274
Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKN E+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNLELTVFYGQYRCSGPGADY 334
Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
GGRVSWSRELTESEA+PF+SL FI A EWL PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372
BLAST of MS012710 vs. ExPASy Swiss-Prot
Match:
Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)
HSP 1 Score: 465.7 bits (1197), Expect = 4.9e-130
Identity = 210/328 (64.02%), Postives = 263/328 (80.18%), Query Frame = 0
Query: 29 EGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCR 88
E E++KWV+ +GS +HS+++ +NK P TL V K + G F +Q AI+S+P++N R
Sbjct: 54 EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113
Query: 89 VVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFA 148
VVI V AGVY+EKV IP ++I +EGEGAEKT +EW DTA G P+GT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173
Query: 149 VNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRH 208
VNSPFFVA+NITF+N VP GA+GKQAVALR+S D AAF CR +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233
Query: 209 YFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSG 268
Y+KDCY+EGSVDF+FG+ LS+Y+GCH+HA+ + GA+TAQ R+S+L+ TGFSFV CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293
Query: 269 SGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGG 328
+G LYLGRAWG FSRVVFAYTYMD II P GWYNWGD +REMTVFYGQYKC+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353
Query: 329 RVSWSRELTESEADPFLSLQFINANEWL 357
RV+W+RELT+ EA PFLSL FI+ +EW+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381
BLAST of MS012710 vs. ExPASy Swiss-Prot
Match:
Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)
HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 139/287 (48.43%), Postives = 187/287 (65.16%), Query Frame = 0
Query: 71 FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
FRSVQ A++S+P N + I + G YREKV +PAT YI +G G + T IEW D A
Sbjct: 71 FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130
Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
+G NG+ L T+ +A+ V + +F ARNI+F N A P G G QAVA RISGD A F
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190
Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 250
C F GAQDTL D GRHYFK+CY+EGS+DF+FG+G S+Y C LH++ + +G++ A R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250
Query: 251 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDK-NRE 310
++TGF+FV C+V+G+G LY+GRA G +SR+V+AYTY D ++ GW +W K N+
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310
Query: 311 MTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
T F+G Y C GPGA VSW+R L A PF++ F+N W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357
BLAST of MS012710 vs. ExPASy Swiss-Prot
Match:
Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)
HSP 1 Score: 296.2 bits (757), Expect = 5.1e-79
Identity = 149/321 (46.42%), Postives = 208/321 (64.80%), Query Frame = 0
Query: 49 KTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATM 108
+ R+++K L L V G F +VQ AI+ VP ++ + +I V +G YREKV +
Sbjct: 80 RLRHQYKTSLVLTVD-LHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139
Query: 109 SYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVP 168
+ + ++G G + T IEW+DTA G+ +F+ FA N F A NI+FKN A P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199
Query: 169 ASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLS 228
G QAVALRI GD AAF C F GAQDTL D GRH+FK+C+++GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259
Query: 229 IYDGCHLHAVTNS-----YGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSR 288
+Y C ++++ G++TAQ R S +Q+GFSFVNCK+ GSG + LGRAWG ++
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319
Query: 289 VVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADP 348
VVF+ TYM IITP GW NWGD +E TV +G++KC GPGADY RV + ++LT+SEA
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379
Query: 349 FLSLQFINANEWLPRPKHTHL 365
F+ + FI+ +EWL +HT++
Sbjct: 380 FIDVSFIDGDEWL---RHTNI 390
BLAST of MS012710 vs. ExPASy Swiss-Prot
Match:
Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)
HSP 1 Score: 280.0 bits (715), Expect = 3.8e-74
Identity = 135/286 (47.20%), Postives = 184/286 (64.34%), Query Frame = 0
Query: 73 SVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEG--AEKTVIEWDDTAD 132
+VQ A++ VP N RV I + G+YREKV +P + YI G A TVI W D A
Sbjct: 94 TVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKAS 153
Query: 133 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 192
+G +G+ LGT+ +A+ ++ S FF A ITF+N V +G G+QAVALRI GD A F
Sbjct: 154 DLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVALRIIGDKAVFY 213
Query: 193 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 252
R +G+QDTL+D G HYF CY++G+VDF+FG+ S+Y C +H+ YGA+ A R
Sbjct: 214 RVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHR 273
Query: 253 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREM 312
+S + TGFSFVNC +SG+G +YLGRAWG +SR V++ ++ IITP GW +W R+
Sbjct: 274 DSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQR 333
Query: 313 TVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
V +G+Y C G GA+ GGRV WS+ LT E PFL +FI ++WL
Sbjct: 334 KVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378
BLAST of MS012710 vs. ExPASy Swiss-Prot
Match:
O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)
HSP 1 Score: 270.8 bits (691), Expect = 2.3e-71
Identity = 133/294 (45.24%), Postives = 190/294 (64.63%), Query Frame = 0
Query: 71 FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
F +VQ A+++V ++ R VI + +G+Y EKV IP T I ++G+G + T I W+DTA
Sbjct: 101 FTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAY 160
Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
GTF AT V FVA+NI+F N A +P G +G QAVA+RI+GD +AF+
Sbjct: 161 SAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 220
Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSY-------- 250
C F GAQDTL+D GRHYFKDCY++GS+DF+FG+ S+Y C + ++ N
Sbjct: 221 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 280
Query: 251 GALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYN 310
GA+TA R+S + +GFSFVNC + G+G ++LGRAW +SRVVF T M +I P GW N
Sbjct: 281 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 340
Query: 311 WGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
+ D +R+ T+FYG+Y CSGPGAD R + ++L E++ ++ FI+ ++WL
Sbjct: 341 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWL 388
BLAST of MS012710 vs. ExPASy TrEMBL
Match:
A0A6J1FW15 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1)
HSP 1 Score: 628.2 bits (1619), Expect = 2.1e-176
Identity = 291/340 (85.59%), Postives = 320/340 (94.12%), Query Frame = 0
Query: 27 EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
+VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK AGGFRSVQKAI S+P++NR
Sbjct: 35 KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 94
Query: 87 CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154
Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214
Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 274
Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKN E+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNLELTVFYGQYRCSGPGADY 334
Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
GGRVSWSRELTESEA+PF+SL FI A EWL PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372
BLAST of MS012710 vs. ExPASy TrEMBL
Match:
A0A0A0KWM1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1)
HSP 1 Score: 627.5 bits (1617), Expect = 3.6e-176
Identity = 295/378 (78.04%), Postives = 335/378 (88.62%), Query Frame = 0
Query: 1 MTPPLL--KLFLLLAVAFPPPA-------AAAASSEVEGEYLKWVKHMGSFRHSLYQKTR 60
M+ PL+ FLL+A+ P A S V+ EY KW++HMGSF+HSL+Q T+
Sbjct: 1 MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK 60
Query: 61 NKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYI 120
NKFKPCLTL+VSK AGGFRS+QKA+ S+P++NRCRV IHV AG+YREKVEIPATMSYI
Sbjct: 61 NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI 120
Query: 121 WVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASG 180
WVEGEGAEKT+IEW DTADHMG+NGRP+GTFASATFAVNSPFF+A NITFKNKA++P SG
Sbjct: 121 WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG 180
Query: 181 ALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYD 240
ALGKQAVA RISGDAAAFISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIYD
Sbjct: 181 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 240
Query: 241 GCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYM 300
CHLHA+TNSYGALTAQKRNS+L++TGFSF++CKVSGSGALYLGRAWG+FSRVVFAYT+M
Sbjct: 241 SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM 300
Query: 301 DKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFIN 360
DKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADYGGRV WSRELT+SEA+PFLSL FIN
Sbjct: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN 360
Query: 361 ANEWLPRPKHTHLAIIQH 370
AN+WLP HTHL++ H
Sbjct: 361 ANQWLPN-YHTHLSLKPH 377
BLAST of MS012710 vs. ExPASy TrEMBL
Match:
A0A6J1J245 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1)
HSP 1 Score: 627.1 bits (1616), Expect = 4.7e-176
Identity = 297/373 (79.62%), Postives = 330/373 (88.47%), Query Frame = 0
Query: 5 LLKLFLLLAVAFPPPAAAAASSE-----------VEGEYLKWVKHMGSFRHSLYQKTRNK 64
+L F+L+ + P AA + + VE EYLKW++HMGSF+HSL+Q T+NK
Sbjct: 3 MLNFFVLVVMIMLLPGAAVSYGQNWTVGGGGMRRVEDEYLKWIRHMGSFKHSLFQNTKNK 62
Query: 65 FKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWV 124
FKPCLTLRVSK GGFRSVQKAI S+P++NRCRVVIHV AG+YREKVEIPA MSYIWV
Sbjct: 63 FKPCLTLRVSKKPKPGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSYIWV 122
Query: 125 EGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGAL 184
EGEGAEKTVIEW DTADHMGDNGRP+GTF SATFAVNSPFF+ARNITFKNKA+ P SGAL
Sbjct: 123 EGEGAEKTVIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPSGAL 182
Query: 185 GKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGC 244
GKQAVA RI+GDAAAFISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIY+GC
Sbjct: 183 GKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIYEGC 242
Query: 245 HLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDK 304
HLHA+T+SYGALTAQKRNSLL++TGFSF+NCKVSGSGALYLGRAWG+FSRVVFAYT+MDK
Sbjct: 243 HLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDK 302
Query: 305 IITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINAN 364
IITPTGWYNWGDKNRE+TVFYGQY+CSGPGADYGGRVSWSRELTE EA+PF+SL FI A
Sbjct: 303 IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTEPEANPFVSLDFIGAK 362
Query: 365 EWLPRPKHTHLAI 367
EWL PK THLAI
Sbjct: 363 EWL--PKRTHLAI 373
BLAST of MS012710 vs. ExPASy TrEMBL
Match:
A0A1S3CNF2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1)
HSP 1 Score: 626.7 bits (1615), Expect = 6.1e-176
Identity = 293/368 (79.62%), Postives = 330/368 (89.67%), Query Frame = 0
Query: 8 LFLLLAVAFPPPA------AAAASSEVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLR 67
LFLL+A+ P A V+ EYLKW++HMGSF+HSL+Q T+NKFKPCLTL+
Sbjct: 9 LFLLIAIILPAAAQNWTAGGGRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLK 68
Query: 68 VSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKT 127
VSK A GGFRS+QKA+ S+P++NRCRV IHV AG+YREKVEIPATMSYIWVEGEGAEKT
Sbjct: 69 VSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKT 128
Query: 128 VIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALR 187
+IEW DTADHMG+NGRP+GTFASATFAVNSPFF+A NITFKNKA++P SGALGKQAVA R
Sbjct: 129 IIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVAFR 188
Query: 188 ISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNS 247
ISGDAAAFISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIYD CHLHA+TNS
Sbjct: 189 ISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNS 248
Query: 248 YGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWY 307
YGALTAQKRNS+L++TGFSF++CKVSGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWY
Sbjct: 249 YGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWY 308
Query: 308 NWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWLPRPKH 367
NWGDKNRE+TVFYGQY+CSGPGADYGGRVSWSRELT+SEA+PFLSL FINA +WLP H
Sbjct: 309 NWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAKQWLPN-YH 368
Query: 368 THLAIIQH 370
THL+ H
Sbjct: 369 THLSHKPH 375
BLAST of MS012710 vs. ExPASy TrEMBL
Match:
A0A5A7TMQ1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 PE=4 SV=1)
HSP 1 Score: 603.6 bits (1555), Expect = 5.5e-169
Identity = 286/376 (76.06%), Postives = 324/376 (86.17%), Query Frame = 0
Query: 8 LFLLLAVAFPPPA------AAAASSEVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLR 67
LFLL+A+ P A V+ EYLKW++HMGSF+HSL+Q T+NKFKPCLTL+
Sbjct: 9 LFLLIAIILPAAAQNWTAGGGRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLK 68
Query: 68 VSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKT 127
VSK A GGFRS+QKA+ S+P++NRCRV IHV AG+YREKVEIPATMSYIWVEGEGAEKT
Sbjct: 69 VSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKT 128
Query: 128 VIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFK--------NKAKVPASGAL 187
+IEW DTADHMG+NGRP+GTFASATFAVNSPFF+A NITFK + +P +L
Sbjct: 129 IIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKVLTFSSFFLSSLLPLKFSL 188
Query: 188 GKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGC 247
GKQAVA RISGDAA FISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIYD C
Sbjct: 189 GKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSC 248
Query: 248 HLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDK 307
HLHA+TNSYGALTAQKRNS+L++TGFSF++CKVSGSGALYLGRAWG+FSRVVFAYT+MDK
Sbjct: 249 HLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDK 308
Query: 308 IITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINAN 367
IITPTGWYNWGDKNRE+TVFYGQY+CSGPGADYGGRVSWSRELT+SEA+PFLSL FINA
Sbjct: 309 IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAK 368
Query: 368 EWLPRPKHTHLAIIQH 370
+WLP HTHL+ H
Sbjct: 369 QWLPN-YHTHLSHKPH 383
BLAST of MS012710 vs. TAIR 10
Match:
AT5G19730.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 465.7 bits (1197), Expect = 3.5e-131
Identity = 210/328 (64.02%), Postives = 263/328 (80.18%), Query Frame = 0
Query: 29 EGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCR 88
E E++KWV+ +GS +HS+++ +NK P TL V K + G F +Q AI+S+P++N R
Sbjct: 54 EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113
Query: 89 VVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFA 148
VVI V AGVY+EKV IP ++I +EGEGAEKT +EW DTA G P+GT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173
Query: 149 VNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRH 208
VNSPFFVA+NITF+N VP GA+GKQAVALR+S D AAF CR +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233
Query: 209 YFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSG 268
Y+KDCY+EGSVDF+FG+ LS+Y+GCH+HA+ + GA+TAQ R+S+L+ TGFSFV CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293
Query: 269 SGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGG 328
+G LYLGRAWG FSRVVFAYTYMD II P GWYNWGD +REMTVFYGQYKC+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353
Query: 329 RVSWSRELTESEADPFLSLQFINANEWL 357
RV+W+RELT+ EA PFLSL FI+ +EW+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381
BLAST of MS012710 vs. TAIR 10
Match:
AT5G47500.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 298.1 bits (762), Expect = 9.6e-81
Identity = 139/287 (48.43%), Postives = 187/287 (65.16%), Query Frame = 0
Query: 71 FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
FRSVQ A++S+P N + I + G YREKV +PAT YI +G G + T IEW D A
Sbjct: 71 FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130
Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
+G NG+ L T+ +A+ V + +F ARNI+F N A P G G QAVA RISGD A F
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190
Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 250
C F GAQDTL D GRHYFK+CY+EGS+DF+FG+G S+Y C LH++ + +G++ A R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250
Query: 251 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDK-NRE 310
++TGF+FV C+V+G+G LY+GRA G +SR+V+AYTY D ++ GW +W K N+
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310
Query: 311 MTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
T F+G Y C GPGA VSW+R L A PF++ F+N W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357
BLAST of MS012710 vs. TAIR 10
Match:
AT2G36710.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 296.2 bits (757), Expect = 3.6e-80
Identity = 149/321 (46.42%), Postives = 208/321 (64.80%), Query Frame = 0
Query: 49 KTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATM 108
+ R+++K L L V G F +VQ AI+ VP ++ + +I V +G YREKV +
Sbjct: 80 RLRHQYKTSLVLTVD-LHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139
Query: 109 SYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVP 168
+ + ++G G + T IEW+DTA G+ +F+ FA N F A NI+FKN A P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199
Query: 169 ASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLS 228
G QAVALRI GD AAF C F GAQDTL D GRH+FK+C+++GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259
Query: 229 IYDGCHLHAVTNS-----YGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSR 288
+Y C ++++ G++TAQ R S +Q+GFSFVNCK+ GSG + LGRAWG ++
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319
Query: 289 VVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADP 348
VVF+ TYM IITP GW NWGD +E TV +G++KC GPGADY RV + ++LT+SEA
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379
Query: 349 FLSLQFINANEWLPRPKHTHL 365
F+ + FI+ +EWL +HT++
Sbjct: 380 FIDVSFIDGDEWL---RHTNI 390
BLAST of MS012710 vs. TAIR 10
Match:
AT5G55590.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 280.0 bits (715), Expect = 2.7e-75
Identity = 135/286 (47.20%), Postives = 184/286 (64.34%), Query Frame = 0
Query: 73 SVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEG--AEKTVIEWDDTAD 132
+VQ A++ VP N RV I + G+YREKV +P + YI G A TVI W D A
Sbjct: 94 TVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKAS 153
Query: 133 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 192
+G +G+ LGT+ +A+ ++ S FF A ITF+N V +G G+QAVALRI GD A F
Sbjct: 154 DLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVALRIIGDKAVFY 213
Query: 193 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 252
R +G+QDTL+D G HYF CY++G+VDF+FG+ S+Y C +H+ YGA+ A R
Sbjct: 214 RVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHR 273
Query: 253 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREM 312
+S + TGFSFVNC +SG+G +YLGRAWG +SR V++ ++ IITP GW +W R+
Sbjct: 274 DSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQR 333
Query: 313 TVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
V +G+Y C G GA+ GGRV WS+ LT E PFL +FI ++WL
Sbjct: 334 KVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378
BLAST of MS012710 vs. TAIR 10
Match:
AT1G05310.1 (Pectin lyase-like superfamily protein )
HSP 1 Score: 270.8 bits (691), Expect = 1.6e-72
Identity = 133/294 (45.24%), Postives = 190/294 (64.63%), Query Frame = 0
Query: 71 FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
F +VQ A+++V ++ R VI + +G+Y EKV IP T I ++G+G + T I W+DTA
Sbjct: 101 FTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAY 160
Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
GTF AT V FVA+NI+F N A +P G +G QAVA+RI+GD +AF+
Sbjct: 161 SAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 220
Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSY-------- 250
C F GAQDTL+D GRHYFKDCY++GS+DF+FG+ S+Y C + ++ N
Sbjct: 221 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 280
Query: 251 GALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYN 310
GA+TA R+S + +GFSFVNC + G+G ++LGRAW +SRVVF T M +I P GW N
Sbjct: 281 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 340
Query: 311 WGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
+ D +R+ T+FYG+Y CSGPGAD R + ++L E++ ++ FI+ ++WL
Sbjct: 341 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWL 388
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7031236.1 | 5.1e-177 | 86.18 | putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
KAG6600598.1 | 5.1e-177 | 86.18 | putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_023527790.1 | 2.5e-176 | 85.88 | probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
XP_023527781.1 | 2.5e-176 | 85.88 | probable pectinesterase 53 isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
XP_022942305.1 | 4.3e-176 | 85.59 | probable pectinesterase 53 [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q8VYZ3 | 4.9e-130 | 64.02 | Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1 | [more] |
Q8LPF3 | 1.3e-79 | 48.43 | Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1 | [more] |
Q9ZQA3 | 5.1e-79 | 46.42 | Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1 | [more] |
Q9FM79 | 3.8e-74 | 47.20 | Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1 | [more] |
O23038 | 2.3e-71 | 45.24 | Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FW15 | 2.1e-176 | 85.59 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1 | [more] |
A0A0A0KWM1 | 3.6e-176 | 78.04 | Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1 | [more] |
A0A6J1J245 | 4.7e-176 | 79.62 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1 | [more] |
A0A1S3CNF2 | 6.1e-176 | 79.62 | Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1 | [more] |
A0A5A7TMQ1 | 5.5e-169 | 76.06 | Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 ... | [more] |