MS012710 (gene) Bitter gourd (TR) v1

Overview
NameMS012710
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPectinesterase
Locationscaffold63: 2843733 .. 2847357 (-)
RNA-Seq ExpressionMS012710
SyntenyMS012710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCCGCCGCTGCTCAAGCTTTTTCTTCTGCTGGCGGTGGCTTTCCCGCCGCCCGCCGCCGCCGCCGCCTCCAGTGAAGTGGAAGGCGAGTACTTGAAGTGGGTGAAACACATGGGCTCTTTCCGGCACTCGCTTTACCAGAAGACGAGGAACAAGTTCAAGCCGTGTCTGACGCTGAGGGTGTCAAAGGCGGCGGCGGCGGGGGGTTTCCGGTCGGTTCAGAAGGCTATAGAAAGCGTCCCGGTGGTGAACCGGTGCCGGGTGGTGATCCACGTGGGCGCCGGGGTTTACAGGGAGAAGGTGGAGATTCCGGCGACGATGAGCTACATTTGGGTGGAGGGGGAAGGGGCGGAGAAGACGGTTATTGAGTGGGACGACACGGCGGACCATATGGGCGACAATGGCCGTCCCTTGGGCACCTTTGCTTCTGCTACTTTCGCTGTTAATTCTCCCTTCTTCGTCGCTAGGAACATCACCTTTAAGGTAACACTACTTTTTTTTTTACTTTAATCGAGTTACTACACACTTTCTGATGTGTGGCTTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTAGGTATGAATAGTTGTTATTAAAGGGGGACCGTCGCAATGTGACTAGATTTCAAATTTACCTTTGACAGTTAATAAAGACAATCACGTTCGAATATTTTGTGTATAGTGACTTTTTTTTATACAACTAATTTATTAAAATATAATAAAAACAAATCAATAAAACTATACCACATCTCAAAAGGTTAAAATGAGTGTGTAACTATAGGTAGATAAGTATTGTTCTTTCTAATATATTCGTGTTTCTATTCTATAGATAAATGGAGGTAGTAATATTTGAACTATAGGAGTATCTAAAACGATCTAATTTTGGAGATTAAAACAAAGTATTTGAGAGTTTATGGACAAACCAAAATTAAGCATGAAAAATTAGGGCTAAAAAGAGTTAAAACCTATATACATACCTATATTGTTTGACTTTGATTTATATATTTGTAAATATTTTAATTTTGATCAATAAACTTTCTAATGCTCCACTTTTTATCCTTAATCTTTGCGTTAAAAAATTATTTAAGCCTTACTGTCAATTTTTCATTCAAAATGTCAAAGAAGAATTTAGATCTATTTTAAAATTTAATGCCTAATCTACTAATTAAACTACTAAATTAAAATTGATATCATGCCATATTTTTATTAAATAATTTATAGAAAGATCATCTAAATTTCAAAATTTATACCAAAATAAAATAAACCCAAAAGTTTAAAATAAAATTTAGGTCATCTTTTTCTTTTAGATTCATCCATAGCCATAACACTTAAGTAATGAGTTTTATGAATCTTTTAACAAAAGTATTTGAATTTCACTTTTCAAGACGAATTTTTTGGACGATAAATCCAAATTTCCTGAAAAGGTTTTCTTATAATATAATTTTATAAATTATATTATTTCAAGGCTTGCATATACTAATTAAAAATTGGAGGAATAAAGTTTGAGTGTCGTATGTAAATGGTCATTTCCTCGTGACTTTTAATGACCAAACTGACACACATATATAGTTGTTGGAAGGACTTGGACGACGATTAAAAAAAAAATACTATAATCATCGAGGATTAAAACACACCACACAAAAATTAAAATTTTGAATATAATATAAATATAAATAAAGTAGTGAAGAAACAATTAATAAGATTTCCTAAAAAAATAAAAAAATAATAAGAAAGGGTACGTAGTCCAATTTTGTACTTTTGTTCTTTTTATCTGAGAGAATAAATAAATATTAATTCAAATACAGTTTCAAATCTTTGCATTTTTTTTCATTATATTTTTTTGTGTTCAAACTTCAAAAATACAAAACCCTCAACTCTCATTGTTTATCTTTTGGTCCTCCCAACTTTTAAAGTTGCACGTTTAAGCACTTCAACTTTTATTACCATCTAGGTTTAAATCATATTTTGCTTCATGAATATGTCGTAAAGGCATTACATCTTGTTTATTTAGACATCTTTCTATGAGGTAAGTAAATAAATTGTGAGTCTATTGTTGGGTTTTTTTATTAAAATAATTTATAGAAAAAATTAATGAAAAACCAGCTTAAAATGGTGTTTTATATATATTACAATAATGGTGGGATGAGGATTGAACCTCTAGCCTCAGGAGAATGAATCAAGTGCCTTAAATGCTGAGCGAACCTTAAATCATATGTAATATTAAAATATTAGAAGCAAAAATATAATAATGGTATAAGTTCAATTATCAAAATAGTACGTGAAGCTATAACTTACATTTAATTCAATCCTATTTTAACTAAGTTAATTAAGGTTAGAATTGTATCTATTTATTTCCCATATTTCGCTAACATAAGCTTACTTAGGAGTTAAGATGTCTTATTCATTTCTTAGAGGTCAAGAGGTTCGAACTCCATCCTCTCATTTATTATATATAAAAAAAGTTCCTATACATCAAAAATTTCTAATGTAATTGAACTTTCATCACGGTGTGTCATCCCTATATTTTAAAATTTTTCTAATTTATTCCTAAACTCTAAATTTTATTTTTAATAGATATTTACCACGTATTATTTAAGAATTTATGCGGTGGGCTGACTCATCCACCGACTCATCCAATATAAACTCTTAAACAAAATAGTCCAAGTGTCATCTTACTTAAAATTTGATTAGACATAGAATTAAAATTCGTTGCACACCCGACACCAGGAAAAGTTGAATAATCTGTTAGAAAAATCTCTTGGAGTAACCTTTAAATGATTAAAAAAAAAAGAAGCAATATTCATTTCCATTTAAATTTAGAAAAAGGAAAAAGACGATAAAACTTTAGGACCAAAAAGCCATATGTTTGTAAGTATAAATGAGGGTAATTAGGGGAATGAAAAAACAGAACAAGGCGAAGGTGCCGGCGTCGGGGGCGCTGGGGAAGCAAGCGGTGGCGTTGCGGATATCGGGGGACGCGGCGGCGTTCATAAGCTGCAGGTTCATCGGAGCGCAGGACACGCTGTACGACCACGTGGGCCGCCACTACTTCAAGGACTGCTACGTGGAGGGGTCGGTGGACTTCGTGTTCGGCGACGGGCTGTCCATCTACGACGGCTGCCACCTCCACGCCGTGACCAACAGTTACGGCGCCCTCACGGCCCAGAAGCGGAACAGCCTTCTGGACCAGACCGGGTTCTCCTTCGTCAACTGTAAGGTCTCCGGTTCGGGCGCCCTCTACTTGGGCCGGGCCTGGGGCACCTTCTCCAGAGTCGTCTTTGCCTATACTTACATGGATAAAATCATCACTCCCACTGGCTGGTACAACTGGGGAGACAAAAATAGAGAAATGTAATTACCAAATTTCTTTTTTTGCTTTAATTTTATTTTATTTAGAGTATAATATTTTAATTTAAGATTTGGAGGATGAATATGCAGGACGGTGTTTTATGGGCAATATAAGTGTTCGGGGCCGGGAGCTGATTATGGGGGACGAGTTTCGTGGTCCAGAGAACTCACTGAGTCGGAGGCCGACCCGTTTCTCTCTCTACAGTTTATCAATGCAAACGAATGGCTTCCAAGACCAAAACACACTCATCTTGCCATTATTCAACAT

mRNA sequence

ATGACGCCGCCGCTGCTCAAGCTTTTTCTTCTGCTGGCGGTGGCTTTCCCGCCGCCCGCCGCCGCCGCCGCCTCCAGTGAAGTGGAAGGCGAGTACTTGAAGTGGGTGAAACACATGGGCTCTTTCCGGCACTCGCTTTACCAGAAGACGAGGAACAAGTTCAAGCCGTGTCTGACGCTGAGGGTGTCAAAGGCGGCGGCGGCGGGGGGTTTCCGGTCGGTTCAGAAGGCTATAGAAAGCGTCCCGGTGGTGAACCGGTGCCGGGTGGTGATCCACGTGGGCGCCGGGGTTTACAGGGAGAAGGTGGAGATTCCGGCGACGATGAGCTACATTTGGGTGGAGGGGGAAGGGGCGGAGAAGACGGTTATTGAGTGGGACGACACGGCGGACCATATGGGCGACAATGGCCGTCCCTTGGGCACCTTTGCTTCTGCTACTTTCGCTGTTAATTCTCCCTTCTTCGTCGCTAGGAACATCACCTTTAAGAACAAGGCGAAGGTGCCGGCGTCGGGGGCGCTGGGGAAGCAAGCGGTGGCGTTGCGGATATCGGGGGACGCGGCGGCGTTCATAAGCTGCAGGTTCATCGGAGCGCAGGACACGCTGTACGACCACGTGGGCCGCCACTACTTCAAGGACTGCTACGTGGAGGGGTCGGTGGACTTCGTGTTCGGCGACGGGCTGTCCATCTACGACGGCTGCCACCTCCACGCCGTGACCAACAGTTACGGCGCCCTCACGGCCCAGAAGCGGAACAGCCTTCTGGACCAGACCGGGTTCTCCTTCGTCAACTGTAAGGTCTCCGGTTCGGGCGCCCTCTACTTGGGCCGGGCCTGGGGCACCTTCTCCAGAGTCGTCTTTGCCTATACTTACATGGATAAAATCATCACTCCCACTGGCTGGTACAACTGGGGAGACAAAAATAGAGAAATGACGGTGTTTTATGGGCAATATAAGTGTTCGGGGCCGGGAGCTGATTATGGGGGACGAGTTTCGTGGTCCAGAGAACTCACTGAGTCGGAGGCCGACCCGTTTCTCTCTCTACAGTTTATCAATGCAAACGAATGGCTTCCAAGACCAAAACACACTCATCTTGCCATTATTCAACAT

Coding sequence (CDS)

ATGACGCCGCCGCTGCTCAAGCTTTTTCTTCTGCTGGCGGTGGCTTTCCCGCCGCCCGCCGCCGCCGCCGCCTCCAGTGAAGTGGAAGGCGAGTACTTGAAGTGGGTGAAACACATGGGCTCTTTCCGGCACTCGCTTTACCAGAAGACGAGGAACAAGTTCAAGCCGTGTCTGACGCTGAGGGTGTCAAAGGCGGCGGCGGCGGGGGGTTTCCGGTCGGTTCAGAAGGCTATAGAAAGCGTCCCGGTGGTGAACCGGTGCCGGGTGGTGATCCACGTGGGCGCCGGGGTTTACAGGGAGAAGGTGGAGATTCCGGCGACGATGAGCTACATTTGGGTGGAGGGGGAAGGGGCGGAGAAGACGGTTATTGAGTGGGACGACACGGCGGACCATATGGGCGACAATGGCCGTCCCTTGGGCACCTTTGCTTCTGCTACTTTCGCTGTTAATTCTCCCTTCTTCGTCGCTAGGAACATCACCTTTAAGAACAAGGCGAAGGTGCCGGCGTCGGGGGCGCTGGGGAAGCAAGCGGTGGCGTTGCGGATATCGGGGGACGCGGCGGCGTTCATAAGCTGCAGGTTCATCGGAGCGCAGGACACGCTGTACGACCACGTGGGCCGCCACTACTTCAAGGACTGCTACGTGGAGGGGTCGGTGGACTTCGTGTTCGGCGACGGGCTGTCCATCTACGACGGCTGCCACCTCCACGCCGTGACCAACAGTTACGGCGCCCTCACGGCCCAGAAGCGGAACAGCCTTCTGGACCAGACCGGGTTCTCCTTCGTCAACTGTAAGGTCTCCGGTTCGGGCGCCCTCTACTTGGGCCGGGCCTGGGGCACCTTCTCCAGAGTCGTCTTTGCCTATACTTACATGGATAAAATCATCACTCCCACTGGCTGGTACAACTGGGGAGACAAAAATAGAGAAATGACGGTGTTTTATGGGCAATATAAGTGTTCGGGGCCGGGAGCTGATTATGGGGGACGAGTTTCGTGGTCCAGAGAACTCACTGAGTCGGAGGCCGACCCGTTTCTCTCTCTACAGTTTATCAATGCAAACGAATGGCTTCCAAGACCAAAACACACTCATCTTGCCATTATTCAACAT

Protein sequence

MTPPLLKLFLLLAVAFPPPAAAAASSEVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAIIQH
Homology
BLAST of MS012710 vs. NCBI nr
Match: KAG7031236.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 631.3 bits (1627), Expect = 5.1e-177
Identity = 293/340 (86.18%), Postives = 321/340 (94.41%), Query Frame = 0

Query: 27  EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
           +VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK   AGGFRSVQKAI S+P+VNR
Sbjct: 35  KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNR 94

Query: 87  CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
           CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95  CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154

Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
           FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214

Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
           RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 274

Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
           SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 334

Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
           GGRVSWSRELTESEA+PF+SL FI A EWL  PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372

BLAST of MS012710 vs. NCBI nr
Match: KAG6600598.1 (putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 631.3 bits (1627), Expect = 5.1e-177
Identity = 293/340 (86.18%), Postives = 321/340 (94.41%), Query Frame = 0

Query: 27  EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
           +VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK   AGGFRSVQKAI S+P+VNR
Sbjct: 72  KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLVNR 131

Query: 87  CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
           CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 132 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 191

Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
           FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 192 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 251

Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
           RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 252 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 311

Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
           SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 312 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 371

Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
           GGRVSWSRELTESEA+PF+SL FI A EWL  PK THLAI
Sbjct: 372 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 409

BLAST of MS012710 vs. NCBI nr
Match: XP_023527790.1 (probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.5e-176
Identity = 292/340 (85.88%), Postives = 320/340 (94.12%), Query Frame = 0

Query: 27  EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
           +VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK   AGGFRSVQKAI S+P++NR
Sbjct: 35  KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 94

Query: 87  CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
           CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95  CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154

Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
           FAVNSPFFVARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFVARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214

Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
           RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+ CKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKV 274

Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
           SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 334

Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
           GGRVSWSRELTESEA+PF+SL FI A EWL  PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372

BLAST of MS012710 vs. NCBI nr
Match: XP_023527781.1 (probable pectinesterase 53 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.5e-176
Identity = 292/340 (85.88%), Postives = 320/340 (94.12%), Query Frame = 0

Query: 27  EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
           +VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK   AGGFRSVQKAI S+P++NR
Sbjct: 62  KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 121

Query: 87  CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
           CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 122 CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 181

Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
           FAVNSPFFVARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 182 FAVNSPFFVARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 241

Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
           RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+ CKV
Sbjct: 242 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLKCKV 301

Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
           SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADY
Sbjct: 302 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADY 361

Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
           GGRVSWSRELTESEA+PF+SL FI A EWL  PK THLAI
Sbjct: 362 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 399

BLAST of MS012710 vs. NCBI nr
Match: XP_022942305.1 (probable pectinesterase 53 [Cucurbita moschata])

HSP 1 Score: 628.2 bits (1619), Expect = 4.3e-176
Identity = 291/340 (85.59%), Postives = 320/340 (94.12%), Query Frame = 0

Query: 27  EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
           +VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK   AGGFRSVQKAI S+P++NR
Sbjct: 35  KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 94

Query: 87  CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
           CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95  CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154

Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
           FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214

Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
           RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 274

Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
           SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKN E+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNLELTVFYGQYRCSGPGADY 334

Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
           GGRVSWSRELTESEA+PF+SL FI A EWL  PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372

BLAST of MS012710 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.9e-130
Identity = 210/328 (64.02%), Postives = 263/328 (80.18%), Query Frame = 0

Query: 29  EGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCR 88
           E E++KWV+ +GS +HS+++  +NK  P  TL V K +  G F  +Q AI+S+P++N  R
Sbjct: 54  EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113

Query: 89  VVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFA 148
           VVI V AGVY+EKV IP   ++I +EGEGAEKT +EW DTA      G P+GT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173

Query: 149 VNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRH 208
           VNSPFFVA+NITF+N   VP  GA+GKQAVALR+S D AAF  CR +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233

Query: 209 YFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSG 268
           Y+KDCY+EGSVDF+FG+ LS+Y+GCH+HA+ +  GA+TAQ R+S+L+ TGFSFV CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293

Query: 269 SGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGG 328
           +G LYLGRAWG FSRVVFAYTYMD II P GWYNWGD +REMTVFYGQYKC+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353

Query: 329 RVSWSRELTESEADPFLSLQFINANEWL 357
           RV+W+RELT+ EA PFLSL FI+ +EW+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of MS012710 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 139/287 (48.43%), Postives = 187/287 (65.16%), Query Frame = 0

Query: 71  FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
           FRSVQ A++S+P  N   + I +  G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
            +G NG+ L T+ +A+  V + +F ARNI+F N A  P  G  G QAVA RISGD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 250
            C F GAQDTL D  GRHYFK+CY+EGS+DF+FG+G S+Y  C LH++ + +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 251 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDK-NRE 310
               ++TGF+FV C+V+G+G LY+GRA G +SR+V+AYTY D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 311 MTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
            T F+G Y C GPGA     VSW+R L    A PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of MS012710 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 5.1e-79
Identity = 149/321 (46.42%), Postives = 208/321 (64.80%), Query Frame = 0

Query: 49  KTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATM 108
           + R+++K  L L V      G F +VQ AI+ VP ++  + +I V +G YREKV +    
Sbjct: 80  RLRHQYKTSLVLTVD-LHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139

Query: 109 SYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVP 168
           + + ++G G + T IEW+DTA   G+      +F+   FA N   F A NI+FKN A  P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199

Query: 169 ASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLS 228
             G    QAVALRI GD AAF  C F GAQDTL D  GRH+FK+C+++GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259

Query: 229 IYDGCHLHAVTNS-----YGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSR 288
           +Y  C ++++         G++TAQ R S  +Q+GFSFVNCK+ GSG + LGRAWG ++ 
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319

Query: 289 VVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADP 348
           VVF+ TYM  IITP GW NWGD  +E TV +G++KC GPGADY  RV + ++LT+SEA  
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379

Query: 349 FLSLQFINANEWLPRPKHTHL 365
           F+ + FI+ +EWL   +HT++
Sbjct: 380 FIDVSFIDGDEWL---RHTNI 390

BLAST of MS012710 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 3.8e-74
Identity = 135/286 (47.20%), Postives = 184/286 (64.34%), Query Frame = 0

Query: 73  SVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEG--AEKTVIEWDDTAD 132
           +VQ A++ VP  N  RV I +  G+YREKV +P +  YI   G    A  TVI W D A 
Sbjct: 94  TVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKAS 153

Query: 133 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 192
            +G +G+ LGT+ +A+ ++ S FF A  ITF+N   V  +G  G+QAVALRI GD A F 
Sbjct: 154 DLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVALRIIGDKAVFY 213

Query: 193 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 252
             R +G+QDTL+D  G HYF  CY++G+VDF+FG+  S+Y  C +H+    YGA+ A  R
Sbjct: 214 RVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHR 273

Query: 253 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREM 312
           +S  + TGFSFVNC +SG+G +YLGRAWG +SR V++  ++  IITP GW +W    R+ 
Sbjct: 274 DSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQR 333

Query: 313 TVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
            V +G+Y C G GA+ GGRV WS+ LT  E  PFL  +FI  ++WL
Sbjct: 334 KVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

BLAST of MS012710 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 2.3e-71
Identity = 133/294 (45.24%), Postives = 190/294 (64.63%), Query Frame = 0

Query: 71  FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
           F +VQ A+++V   ++ R VI + +G+Y EKV IP T   I ++G+G + T I W+DTA 
Sbjct: 101 FTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAY 160

Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
                    GTF  AT  V    FVA+NI+F N A +P  G +G QAVA+RI+GD +AF+
Sbjct: 161 SAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 220

Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSY-------- 250
            C F GAQDTL+D  GRHYFKDCY++GS+DF+FG+  S+Y  C + ++ N          
Sbjct: 221 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 280

Query: 251 GALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYN 310
           GA+TA  R+S  + +GFSFVNC + G+G ++LGRAW  +SRVVF  T M  +I P GW N
Sbjct: 281 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 340

Query: 311 WGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
           + D +R+ T+FYG+Y CSGPGAD   R  + ++L E++    ++  FI+ ++WL
Sbjct: 341 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWL 388

BLAST of MS012710 vs. ExPASy TrEMBL
Match: A0A6J1FW15 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 2.1e-176
Identity = 291/340 (85.59%), Postives = 320/340 (94.12%), Query Frame = 0

Query: 27  EVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNR 86
           +VE EYLKW++HMGSF+HSL+Q T+NKFKPCLTLRVSK   AGGFRSVQKAI S+P++NR
Sbjct: 35  KVEDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLRVSKKPKAGGFRSVQKAINSLPLINR 94

Query: 87  CRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASAT 146
           CRVVIHV AG+YREKVEIPA MSYIWVEGEGAEKT+IEW DTADHMGDNGRP+GTF SAT
Sbjct: 95  CRVVIHVAAGIYREKVEIPAMMSYIWVEGEGAEKTIIEWSDTADHMGDNGRPIGTFGSAT 154

Query: 147 FAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVG 206
           FAVNSPFF+ARNITFKNKA+ P SGALGKQAVA RI+GDAAAFISCRFIGAQDTLYDH+G
Sbjct: 155 FAVNSPFFIARNITFKNKARAPPSGALGKQAVAFRITGDAAAFISCRFIGAQDTLYDHLG 214

Query: 207 RHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKV 266
           RHYFKDCY+EGSVDFVFGDGLSIY+GCHLHA+T+SYGALTAQKRNSLL++TGFSF+NCKV
Sbjct: 215 RHYFKDCYIEGSVDFVFGDGLSIYEGCHLHAITDSYGALTAQKRNSLLEETGFSFLNCKV 274

Query: 267 SGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADY 326
           SGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWYNWGDKN E+TVFYGQY+CSGPGADY
Sbjct: 275 SGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWYNWGDKNLELTVFYGQYRCSGPGADY 334

Query: 327 GGRVSWSRELTESEADPFLSLQFINANEWLPRPKHTHLAI 367
           GGRVSWSRELTESEA+PF+SL FI A EWL  PK THLAI
Sbjct: 335 GGRVSWSRELTESEANPFVSLDFIGAKEWL--PKRTHLAI 372

BLAST of MS012710 vs. ExPASy TrEMBL
Match: A0A0A0KWM1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.6e-176
Identity = 295/378 (78.04%), Postives = 335/378 (88.62%), Query Frame = 0

Query: 1   MTPPLL--KLFLLLAVAFPPPA-------AAAASSEVEGEYLKWVKHMGSFRHSLYQKTR 60
           M+ PL+    FLL+A+  P  A            S V+ EY KW++HMGSF+HSL+Q T+
Sbjct: 1   MSRPLIFNLFFLLIAIILPAAAQNWTAGGGRGMRSTVQDEYFKWIRHMGSFKHSLFQNTK 60

Query: 61  NKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYI 120
           NKFKPCLTL+VSK   AGGFRS+QKA+ S+P++NRCRV IHV AG+YREKVEIPATMSYI
Sbjct: 61  NKFKPCLTLKVSKNTKAGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYI 120

Query: 121 WVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASG 180
           WVEGEGAEKT+IEW DTADHMG+NGRP+GTFASATFAVNSPFF+A NITFKNKA++P SG
Sbjct: 121 WVEGEGAEKTIIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSG 180

Query: 181 ALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYD 240
           ALGKQAVA RISGDAAAFISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIYD
Sbjct: 181 ALGKQAVAFRISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYD 240

Query: 241 GCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYM 300
            CHLHA+TNSYGALTAQKRNS+L++TGFSF++CKVSGSGALYLGRAWG+FSRVVFAYT+M
Sbjct: 241 SCHLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFM 300

Query: 301 DKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFIN 360
           DKIITPTGWYNWGDKNRE+TVFYGQY+CSGPGADYGGRV WSRELT+SEA+PFLSL FIN
Sbjct: 301 DKIITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVPWSRELTQSEANPFLSLDFIN 360

Query: 361 ANEWLPRPKHTHLAIIQH 370
           AN+WLP   HTHL++  H
Sbjct: 361 ANQWLPN-YHTHLSLKPH 377

BLAST of MS012710 vs. ExPASy TrEMBL
Match: A0A6J1J245 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.7e-176
Identity = 297/373 (79.62%), Postives = 330/373 (88.47%), Query Frame = 0

Query: 5   LLKLFLLLAVAFPPPAAAAASSE-----------VEGEYLKWVKHMGSFRHSLYQKTRNK 64
           +L  F+L+ +    P AA +  +           VE EYLKW++HMGSF+HSL+Q T+NK
Sbjct: 3   MLNFFVLVVMIMLLPGAAVSYGQNWTVGGGGMRRVEDEYLKWIRHMGSFKHSLFQNTKNK 62

Query: 65  FKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWV 124
           FKPCLTLRVSK    GGFRSVQKAI S+P++NRCRVVIHV AG+YREKVEIPA MSYIWV
Sbjct: 63  FKPCLTLRVSKKPKPGGFRSVQKAINSLPLINRCRVVIHVAAGIYREKVEIPAMMSYIWV 122

Query: 125 EGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGAL 184
           EGEGAEKTVIEW DTADHMGDNGRP+GTF SATFAVNSPFF+ARNITFKNKA+ P SGAL
Sbjct: 123 EGEGAEKTVIEWSDTADHMGDNGRPIGTFGSATFAVNSPFFIARNITFKNKARAPPSGAL 182

Query: 185 GKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGC 244
           GKQAVA RI+GDAAAFISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIY+GC
Sbjct: 183 GKQAVAFRITGDAAAFISCRFIGAQDTLYDHLGRHYFKDCYIEGSVDFVFGDGLSIYEGC 242

Query: 245 HLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDK 304
           HLHA+T+SYGALTAQKRNSLL++TGFSF+NCKVSGSGALYLGRAWG+FSRVVFAYT+MDK
Sbjct: 243 HLHAITDSYGALTAQKRNSLLEETGFSFLNCKVSGSGALYLGRAWGSFSRVVFAYTFMDK 302

Query: 305 IITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINAN 364
           IITPTGWYNWGDKNRE+TVFYGQY+CSGPGADYGGRVSWSRELTE EA+PF+SL FI A 
Sbjct: 303 IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTEPEANPFVSLDFIGAK 362

Query: 365 EWLPRPKHTHLAI 367
           EWL  PK THLAI
Sbjct: 363 EWL--PKRTHLAI 373

BLAST of MS012710 vs. ExPASy TrEMBL
Match: A0A1S3CNF2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 6.1e-176
Identity = 293/368 (79.62%), Postives = 330/368 (89.67%), Query Frame = 0

Query: 8   LFLLLAVAFPPPA------AAAASSEVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLR 67
           LFLL+A+  P  A             V+ EYLKW++HMGSF+HSL+Q T+NKFKPCLTL+
Sbjct: 9   LFLLIAIILPAAAQNWTAGGGRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLK 68

Query: 68  VSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKT 127
           VSK A  GGFRS+QKA+ S+P++NRCRV IHV AG+YREKVEIPATMSYIWVEGEGAEKT
Sbjct: 69  VSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKT 128

Query: 128 VIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALR 187
           +IEW DTADHMG+NGRP+GTFASATFAVNSPFF+A NITFKNKA++P SGALGKQAVA R
Sbjct: 129 IIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKNKARLPPSGALGKQAVAFR 188

Query: 188 ISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNS 247
           ISGDAAAFISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIYD CHLHA+TNS
Sbjct: 189 ISGDAAAFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSCHLHAITNS 248

Query: 248 YGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWY 307
           YGALTAQKRNS+L++TGFSF++CKVSGSGALYLGRAWG+FSRVVFAYT+MDKIITPTGWY
Sbjct: 249 YGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDKIITPTGWY 308

Query: 308 NWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWLPRPKH 367
           NWGDKNRE+TVFYGQY+CSGPGADYGGRVSWSRELT+SEA+PFLSL FINA +WLP   H
Sbjct: 309 NWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAKQWLPN-YH 368

Query: 368 THLAIIQH 370
           THL+   H
Sbjct: 369 THLSHKPH 375

BLAST of MS012710 vs. ExPASy TrEMBL
Match: A0A5A7TMQ1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 PE=4 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.5e-169
Identity = 286/376 (76.06%), Postives = 324/376 (86.17%), Query Frame = 0

Query: 8   LFLLLAVAFPPPA------AAAASSEVEGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLR 67
           LFLL+A+  P  A             V+ EYLKW++HMGSF+HSL+Q T+NKFKPCLTL+
Sbjct: 9   LFLLIAIILPAAAQNWTAGGGRGMRTVQDEYLKWIRHMGSFKHSLFQNTKNKFKPCLTLK 68

Query: 68  VSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKT 127
           VSK A  GGFRS+QKA+ S+P++NRCRV IHV AG+YREKVEIPATMSYIWVEGEGAEKT
Sbjct: 69  VSKNAKNGGFRSLQKAVNSLPIINRCRVRIHVAAGIYREKVEIPATMSYIWVEGEGAEKT 128

Query: 128 VIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFK--------NKAKVPASGAL 187
           +IEW DTADHMG+NGRP+GTFASATFAVNSPFF+A NITFK          + +P   +L
Sbjct: 129 IIEWGDTADHMGENGRPMGTFASATFAVNSPFFIATNITFKVLTFSSFFLSSLLPLKFSL 188

Query: 188 GKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGC 247
           GKQAVA RISGDAA FISCRFIGAQDTLYDH+GRHYFKDCY+EGSVDFVFGDGLSIYD C
Sbjct: 189 GKQAVAFRISGDAATFISCRFIGAQDTLYDHMGRHYFKDCYIEGSVDFVFGDGLSIYDSC 248

Query: 248 HLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDK 307
           HLHA+TNSYGALTAQKRNS+L++TGFSF++CKVSGSGALYLGRAWG+FSRVVFAYT+MDK
Sbjct: 249 HLHAITNSYGALTAQKRNSMLEETGFSFLHCKVSGSGALYLGRAWGSFSRVVFAYTFMDK 308

Query: 308 IITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINAN 367
           IITPTGWYNWGDKNRE+TVFYGQY+CSGPGADYGGRVSWSRELT+SEA+PFLSL FINA 
Sbjct: 309 IITPTGWYNWGDKNRELTVFYGQYRCSGPGADYGGRVSWSRELTQSEANPFLSLDFINAK 368

Query: 368 EWLPRPKHTHLAIIQH 370
           +WLP   HTHL+   H
Sbjct: 369 QWLPN-YHTHLSHKPH 383

BLAST of MS012710 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 465.7 bits (1197), Expect = 3.5e-131
Identity = 210/328 (64.02%), Postives = 263/328 (80.18%), Query Frame = 0

Query: 29  EGEYLKWVKHMGSFRHSLYQKTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCR 88
           E E++KWV+ +GS +HS+++  +NK  P  TL V K +  G F  +Q AI+S+P++N  R
Sbjct: 54  EDEFMKWVRFVGSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVR 113

Query: 89  VVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFA 148
           VVI V AGVY+EKV IP   ++I +EGEGAEKT +EW DTA      G P+GT+ SA+FA
Sbjct: 114 VVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFA 173

Query: 149 VNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRH 208
           VNSPFFVA+NITF+N   VP  GA+GKQAVALR+S D AAF  CR +GAQDTLYDH+GRH
Sbjct: 174 VNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRH 233

Query: 209 YFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKRNSLLDQTGFSFVNCKVSG 268
           Y+KDCY+EGSVDF+FG+ LS+Y+GCH+HA+ +  GA+TAQ R+S+L+ TGFSFV CKV+G
Sbjct: 234 YYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAVTAQGRSSVLEDTGFSFVKCKVTG 293

Query: 269 SGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGG 328
           +G LYLGRAWG FSRVVFAYTYMD II P GWYNWGD +REMTVFYGQYKC+G GA+YGG
Sbjct: 294 TGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGG 353

Query: 329 RVSWSRELTESEADPFLSLQFINANEWL 357
           RV+W+RELT+ EA PFLSL FI+ +EW+
Sbjct: 354 RVAWARELTDEEAKPFLSLTFIDGSEWI 381

BLAST of MS012710 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 9.6e-81
Identity = 139/287 (48.43%), Postives = 187/287 (65.16%), Query Frame = 0

Query: 71  FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
           FRSVQ A++S+P  N   + I +  G YREKV +PAT  YI  +G G + T IEW D A 
Sbjct: 71  FRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDVTAIEWHDRAS 130

Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
            +G NG+ L T+ +A+  V + +F ARNI+F N A  P  G  G QAVA RISGD A F 
Sbjct: 131 DLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAFRISGDKAFFS 190

Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 250
            C F GAQDTL D  GRHYFK+CY+EGS+DF+FG+G S+Y  C LH++ + +G++ A  R
Sbjct: 191 GCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIASRFGSIAAHGR 250

Query: 251 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDK-NRE 310
               ++TGF+FV C+V+G+G LY+GRA G +SR+V+AYTY D ++   GW +W  K N+ 
Sbjct: 251 TCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKS 310

Query: 311 MTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
            T F+G Y C GPGA     VSW+R L    A PF++  F+N   W+
Sbjct: 311 KTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of MS012710 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.6e-80
Identity = 149/321 (46.42%), Postives = 208/321 (64.80%), Query Frame = 0

Query: 49  KTRNKFKPCLTLRVSKAAAAGGFRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATM 108
           + R+++K  L L V      G F +VQ AI+ VP ++  + +I V +G YREKV +    
Sbjct: 80  RLRHQYKTSLVLTVD-LHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENK 139

Query: 109 SYIWVEGEGAEKTVIEWDDTADHMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVP 168
           + + ++G G + T IEW+DTA   G+      +F+   FA N   F A NI+FKN A  P
Sbjct: 140 TNLVIQGRGYQNTSIEWNDTAKSAGNTA---DSFSFVVFAAN---FTAYNISFKNNAPEP 199

Query: 169 ASGALGKQAVALRISGDAAAFISCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLS 228
             G    QAVALRI GD AAF  C F GAQDTL D  GRH+FK+C+++GS+DF+FG+G S
Sbjct: 200 DPGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRS 259

Query: 229 IYDGCHLHAVTNS-----YGALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSR 288
           +Y  C ++++         G++TAQ R S  +Q+GFSFVNCK+ GSG + LGRAWG ++ 
Sbjct: 260 LYQDCTINSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYAT 319

Query: 289 VVFAYTYMDKIITPTGWYNWGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADP 348
           VVF+ TYM  IITP GW NWGD  +E TV +G++KC GPGADY  RV + ++LT+SEA  
Sbjct: 320 VVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASS 379

Query: 349 FLSLQFINANEWLPRPKHTHL 365
           F+ + FI+ +EWL   +HT++
Sbjct: 380 FIDVSFIDGDEWL---RHTNI 390

BLAST of MS012710 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.7e-75
Identity = 135/286 (47.20%), Postives = 184/286 (64.34%), Query Frame = 0

Query: 73  SVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEG--AEKTVIEWDDTAD 132
           +VQ A++ VP  N  RV I +  G+YREKV +P +  YI   G    A  TVI W D A 
Sbjct: 94  TVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKAS 153

Query: 133 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 192
            +G +G+ LGT+ +A+ ++ S FF A  ITF+N   V  +G  G+QAVALRI GD A F 
Sbjct: 154 DLGCDGKELGTYRTASVSIESDFFCATAITFENTV-VAEAGEQGRQAVALRIIGDKAVFY 213

Query: 193 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSYGALTAQKR 252
             R +G+QDTL+D  G HYF  CY++G+VDF+FG+  S+Y  C +H+    YGA+ A  R
Sbjct: 214 RVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAKRYGAIAAHHR 273

Query: 253 NSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYNWGDKNREM 312
           +S  + TGFSFVNC +SG+G +YLGRAWG +SR V++  ++  IITP GW +W    R+ 
Sbjct: 274 DSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWKHPERQR 333

Query: 313 TVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
            V +G+Y C G GA+ GGRV WS+ LT  E  PFL  +FI  ++WL
Sbjct: 334 KVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

BLAST of MS012710 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.6e-72
Identity = 133/294 (45.24%), Postives = 190/294 (64.63%), Query Frame = 0

Query: 71  FRSVQKAIESVPVVNRCRVVIHVGAGVYREKVEIPATMSYIWVEGEGAEKTVIEWDDTAD 130
           F +VQ A+++V   ++ R VI + +G+Y EKV IP T   I ++G+G + T I W+DTA 
Sbjct: 101 FTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIAWNDTAY 160

Query: 131 HMGDNGRPLGTFASATFAVNSPFFVARNITFKNKAKVPASGALGKQAVALRISGDAAAFI 190
                    GTF  AT  V    FVA+NI+F N A +P  G +G QAVA+RI+GD +AF+
Sbjct: 161 SAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 220

Query: 191 SCRFIGAQDTLYDHVGRHYFKDCYVEGSVDFVFGDGLSIYDGCHLHAVTNSY-------- 250
            C F GAQDTL+D  GRHYFKDCY++GS+DF+FG+  S+Y  C + ++ N          
Sbjct: 221 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 280

Query: 251 GALTAQKRNSLLDQTGFSFVNCKVSGSGALYLGRAWGTFSRVVFAYTYMDKIITPTGWYN 310
           GA+TA  R+S  + +GFSFVNC + G+G ++LGRAW  +SRVVF  T M  +I P GW N
Sbjct: 281 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 340

Query: 311 WGDKNREMTVFYGQYKCSGPGADYGGRVSWSRELTESEADPFLSLQFINANEWL 357
           + D +R+ T+FYG+Y CSGPGAD   R  + ++L E++    ++  FI+ ++WL
Sbjct: 341 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWL 388

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031236.15.1e-17786.18putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6600598.15.1e-17786.18putative pectinesterase 53, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023527790.12.5e-17685.88probable pectinesterase 53 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023527781.12.5e-17685.88probable pectinesterase 53 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022942305.14.3e-17685.59probable pectinesterase 53 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8VYZ34.9e-13064.02Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q8LPF31.3e-7948.43Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9ZQA35.1e-7946.42Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q9FM793.8e-7447.20Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
O230382.3e-7145.24Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FW152.1e-17685.59Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447391 PE=4 SV=1[more]
A0A0A0KWM13.6e-17678.04Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G043950 PE=4 SV=1[more]
A0A6J1J2454.7e-17679.62Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480563 PE=4 SV=1[more]
A0A1S3CNF26.1e-17679.62Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103502900 PE=4 SV=1[more]
A0A5A7TMQ15.5e-16976.06Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00630 ... [more]
Match NameE-valueIdentityDescription
AT5G19730.13.5e-13164.02Pectin lyase-like superfamily protein [more]
AT5G47500.19.6e-8148.43Pectin lyase-like superfamily protein [more]
AT2G36710.13.6e-8046.42Pectin lyase-like superfamily protein [more]
AT5G55590.12.7e-7547.20Pectin lyase-like superfamily protein [more]
AT1G05310.11.6e-7245.24Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 59..364
e-value: 2.4E-101
score: 341.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 67..352
e-value: 5.1E-73
score: 245.4
NoneNo IPR availablePANTHERPTHR31321:SF103PECTINESTERASEcoord: 26..357
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 26..357
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 215..224
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 59..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012710.1MS012710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
molecular_function GO:0030599 pectinesterase activity