MS012631 (gene) Bitter gourd (TR) v1

Overview
NameMS012631
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionDolichol-phosphate mannosyltransferase subunit 3
Locationscaffold63: 2187105 .. 2187624 (-)
RNA-Seq ExpressionMS012631
SyntenyMS012631
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCATATTGTCAAGATTTTGACATTATTGGTGGCCATATCAGCCCTGTGGATTGGCCTCTTACAGACATCAGTTTTACCACATAACCATGCTTGGTTGGTGAGTTGACATTCAGTCATTATTTTCTTAAATCCTTCATTTTTATTTCATCATTTTACTCTCAATTGTTCTTACTATGCATGTTTTTATCACGCCAGTTACCCATCTATTTAATTGTCTCCCTCGGATGCTATGGTCTGTTAATGGTTGGAGTCGGTCTAATGACATTTCCAACTTGCCCCAGAGAAGGACTGTTGTTACAGCAGGTATCGCCTCTCTAATTTCCACTTGGCACTTTCATACCATACAACAAATGACCCTCTCTCTCTCTCTGGAATGTTCTTCAAATTTTCCATATCATACATCATTACTAAGACTGGAATGTTCTCATTTGGAACCTCTCTCTCTCTCTCTCTTGGATTCCTTTTGTAGGACATTGATGAGGCCAAAGAGTACCTTAAACAAAAAGGGGTTGACA

mRNA sequence

ATGAAGCATATTGTCAAGATTTTGACATTATTGGTGGCCATATCAGCCCTGTGGATTGGCCTCTTACAGACATCAGTTTTACCACATAACCATGCTTGGTTGTTACCCATCTATTTAATTGTCTCCCTCGGATGCTATGGTCTGTTAATGGTTGGAGTCGGTCTAATGACATTTCCAACTTGCCCCAGAGAAGGACTGTTGTTACAGCAGGTAGCCTCTCTAATTTCCACTTGGCACTTTCATACCATACAACAAATGACCCTCTCTCTCTCTCTGGAATGTTCTTCAAATTTTCCATATCATACATCATTACTAAGACTGGAATGTTCTCATTTGGAACCTCTCTCTCTCTCTCTCTTGGATTCCTTTTGTAGGACATTGATGAGGCCAAAGAGTACCTTAAACAAAAAGGGGTTGACA

Coding sequence (CDS)

ATGAAGCATATTGTCAAGATTTTGACATTATTGGTGGCCATATCAGCCCTGTGGATTGGCCTCTTACAGACATCAGTTTTACCACATAACCATGCTTGGTTGTTACCCATCTATTTAATTGTCTCCCTCGGATGCTATGGTCTGTTAATGGTTGGAGTCGGTCTAATGACATTTCCAACTTGCCCCAGAGAAGGACTGTTGTTACAGCAGGTAGCCTCTCTAATTTCCACTTGGCACTTTCATACCATACAACAAATGACCCTCTCTCTCTCTCTGGAATGTTCTTCAAATTTTCCATATCATACATCATTACTAAGACTGGAATGTTCTCATTTGGAACCTCTCTCTCTCTCTCTCTTGGATTCCTTTTGTAGGACATTGATGAGGCCAAAGAGTACCTTAAACAAAAAGGGGTTGACA

Protein sequence

MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPTCPREGLLLQQVASLISTWHFHTIQQMTLSLSLECSSNFPYHTSLLRLECSHLEPLSLSLLDSFCRTLMRPKSTLNKKGLT
Homology
BLAST of MS012631 vs. NCBI nr
Match: XP_008465116.1 (PREDICTED: uncharacterized protein LOC103502803 isoform X4 [Cucumis melo] >XP_008465117.1 PREDICTED: uncharacterized protein LOC103502803 isoform X4 [Cucumis melo])

HSP 1 Score: 150.6 bits (379), Expect = 1.0e-32
Identity = 94/146 (64.38%), Postives = 96/146 (65.75%), Query Frame = 0

Query: 1   MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
           MKHIVKILTLLVAISALWIGLLQTS+LPH H WLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 1   MKHIVKILTLLVAISALWIGLLQTSILPHGHTWLLPIYFIVSLGCYGLLMVGVGLMTFPT 60

Query: 61  CPREGLLLQQV-ASLISTWHFHTIQQMT-LSLSLE----CSSNFPYHTSLLRLECSHLEP 120
           CP EGLLLQ+V     S  HF  IQQ T  SLSL       S FPY              
Sbjct: 61  CPAEGLLLQKVLLPFYSHLHFRVIQQRTPSSLSLPFFSGMFSKFPYP------------- 120

Query: 121 LSLSLLDSFCRTLMRPKSTLNKKGLT 141
                     RTLMRPKS L KKGLT
Sbjct: 121 ----------RTLMRPKSILRKKGLT 123

BLAST of MS012631 vs. NCBI nr
Match: XP_022136354.1 (dolichol-phosphate mannose synthase subunit 3 [Momordica charantia])

HSP 1 Score: 139.0 bits (349), Expect = 3.0e-29
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT
Sbjct: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60

Query: 61 CPREGLLLQQ 71
          CPREGLLLQQ
Sbjct: 61 CPREGLLLQQ 70

BLAST of MS012631 vs. NCBI nr
Match: XP_022941634.1 (dolichol-phosphate mannose synthase subunit 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 133.7 bits (335), Expect = 1.3e-27
Identity = 66/70 (94.29%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1   MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
           MKHIVKILTLL+AISALWIGLLQTS+LPH HAWLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 32  MKHIVKILTLLMAISALWIGLLQTSILPHGHAWLLPIYFIVSLGCYGLLMVGVGLMTFPT 91

Query: 61  CPREGLLLQQ 71
           CPREGLLLQQ
Sbjct: 92  CPREGLLLQQ 101

BLAST of MS012631 vs. NCBI nr
Match: XP_022941635.1 (dolichol-phosphate mannose synthase subunit 3-like isoform X2 [Cucurbita moschata] >XP_023531536.1 dolichol-phosphate mannose synthase subunit 3-like [Cucurbita pepo subsp. pepo] >XP_023531544.1 dolichol-phosphate mannose synthase subunit 3-like [Cucurbita pepo subsp. pepo] >KAG6600652.1 Dolichol-phosphate mannose synthase subunit 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 133.7 bits (335), Expect = 1.3e-27
Identity = 66/70 (94.29%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKILTLL+AISALWIGLLQTS+LPH HAWLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 1  MKHIVKILTLLMAISALWIGLLQTSILPHGHAWLLPIYFIVSLGCYGLLMVGVGLMTFPT 60

Query: 61 CPREGLLLQQ 71
          CPREGLLLQQ
Sbjct: 61 CPREGLLLQQ 70

BLAST of MS012631 vs. NCBI nr
Match: KAG7031289.1 (Dolichol-phosphate mannose synthase subunit 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.3 bits (334), Expect = 1.7e-27
Identity = 69/90 (76.67%), Postives = 77/90 (85.56%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKILTLL+AISALWIGLLQTS+LPH HAWLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 1  MKHIVKILTLLMAISALWIGLLQTSILPHGHAWLLPIYFIVSLGCYGLLMVGVGLMTFPT 60

Query: 61 CPREGLLLQQVASLISTWHFHTIQQMTLSL 91
          CPREGLLLQQ +   +   F  + Q+++ L
Sbjct: 61 CPREGLLLQQTSPSSA---FSPVDQISICL 87

BLAST of MS012631 vs. ExPASy Swiss-Prot
Match: Q8LEQ4 (Dolichol-phosphate mannose synthase subunit 3 OS=Arabidopsis thaliana OX=3702 GN=DPMS3 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.0e-24
Identity = 54/70 (77.14%), Postives = 63/70 (90.00%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKIL+LLVAISA WIGLLQ +++P +H WLLPIY +VSLGCYGLLMVGVGLM FPT
Sbjct: 1  MKHIVKILSLLVAISAFWIGLLQAAIIPRSHTWLLPIYFVVSLGCYGLLMVGVGLMQFPT 60

Query: 61 CPREGLLLQQ 71
          CP+E +LLQ+
Sbjct: 61 CPQEAVLLQK 70

BLAST of MS012631 vs. ExPASy TrEMBL
Match: A0A1S3CN50 (Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucumis melo OX=3656 GN=LOC103502803 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.9e-33
Identity = 94/146 (64.38%), Postives = 96/146 (65.75%), Query Frame = 0

Query: 1   MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
           MKHIVKILTLLVAISALWIGLLQTS+LPH H WLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 1   MKHIVKILTLLVAISALWIGLLQTSILPHGHTWLLPIYFIVSLGCYGLLMVGVGLMTFPT 60

Query: 61  CPREGLLLQQV-ASLISTWHFHTIQQMT-LSLSLE----CSSNFPYHTSLLRLECSHLEP 120
           CP EGLLLQ+V     S  HF  IQQ T  SLSL       S FPY              
Sbjct: 61  CPAEGLLLQKVLLPFYSHLHFRVIQQRTPSSLSLPFFSGMFSKFPYP------------- 120

Query: 121 LSLSLLDSFCRTLMRPKSTLNKKGLT 141
                     RTLMRPKS L KKGLT
Sbjct: 121 ----------RTLMRPKSILRKKGLT 123

BLAST of MS012631 vs. ExPASy TrEMBL
Match: A0A6J1C432 (Dolichol-phosphate mannosyltransferase subunit 3 OS=Momordica charantia OX=3673 GN=LOC111008085 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.5e-29
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT
Sbjct: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60

Query: 61 CPREGLLLQQ 71
          CPREGLLLQQ
Sbjct: 61 CPREGLLLQQ 70

BLAST of MS012631 vs. ExPASy TrEMBL
Match: A0A6J1FU86 (Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111446935 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 6.2e-28
Identity = 66/70 (94.29%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKILTLL+AISALWIGLLQTS+LPH HAWLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 1  MKHIVKILTLLMAISALWIGLLQTSILPHGHAWLLPIYFIVSLGCYGLLMVGVGLMTFPT 60

Query: 61 CPREGLLLQQ 71
          CPREGLLLQQ
Sbjct: 61 CPREGLLLQQ 70

BLAST of MS012631 vs. ExPASy TrEMBL
Match: A0A6J1FP02 (Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111446935 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 6.2e-28
Identity = 66/70 (94.29%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1   MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
           MKHIVKILTLL+AISALWIGLLQTS+LPH HAWLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 32  MKHIVKILTLLMAISALWIGLLQTSILPHGHAWLLPIYFIVSLGCYGLLMVGVGLMTFPT 91

Query: 61  CPREGLLLQQ 71
           CPREGLLLQQ
Sbjct: 92  CPREGLLLQQ 101

BLAST of MS012631 vs. ExPASy TrEMBL
Match: A0A6J1JY89 (Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111488551 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.4e-27
Identity = 65/70 (92.86%), Postives = 68/70 (97.14%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKILTLL+AISALWIGLLQTS+LPH HAWLLPIY IVSLGCYGLLMVGVGLMTFPT
Sbjct: 1  MKHIVKILTLLMAISALWIGLLQTSILPHGHAWLLPIYFIVSLGCYGLLMVGVGLMTFPT 60

Query: 61 CPREGLLLQQ 71
          CPREG+LLQQ
Sbjct: 61 CPREGVLLQQ 70

BLAST of MS012631 vs. TAIR 10
Match: AT1G48140.1 (dolichol-phosphate mannosyltransferase-related )

HSP 1 Score: 114.4 bits (285), Expect = 7.4e-26
Identity = 54/70 (77.14%), Postives = 63/70 (90.00%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKIL+LLVAISA WIGLLQ +++P +H WLLPIY +VSLGCYGLLMVGVGLM FPT
Sbjct: 1  MKHIVKILSLLVAISAFWIGLLQAAIIPRSHTWLLPIYFVVSLGCYGLLMVGVGLMQFPT 60

Query: 61 CPREGLLLQQ 71
          CP+E +LLQ+
Sbjct: 61 CPQEAVLLQK 70

BLAST of MS012631 vs. TAIR 10
Match: AT1G48140.2 (dolichol-phosphate mannosyltransferase-related )

HSP 1 Score: 114.4 bits (285), Expect = 7.4e-26
Identity = 54/70 (77.14%), Postives = 63/70 (90.00%), Query Frame = 0

Query: 1  MKHIVKILTLLVAISALWIGLLQTSVLPHNHAWLLPIYLIVSLGCYGLLMVGVGLMTFPT 60
          MKHIVKIL+LLVAISA WIGLLQ +++P +H WLLPIY +VSLGCYGLLMVGVGLM FPT
Sbjct: 1  MKHIVKILSLLVAISAFWIGLLQAAIIPRSHTWLLPIYFVVSLGCYGLLMVGVGLMQFPT 60

Query: 61 CPREGLLLQQ 71
          CP+E +LLQ+
Sbjct: 61 CPQEAVLLQK 70

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008465116.11.0e-3264.38PREDICTED: uncharacterized protein LOC103502803 isoform X4 [Cucumis melo] >XP_00... [more]
XP_022136354.13.0e-29100.00dolichol-phosphate mannose synthase subunit 3 [Momordica charantia][more]
XP_022941634.11.3e-2794.29dolichol-phosphate mannose synthase subunit 3-like isoform X1 [Cucurbita moschat... [more]
XP_022941635.11.3e-2794.29dolichol-phosphate mannose synthase subunit 3-like isoform X2 [Cucurbita moschat... [more]
KAG7031289.11.7e-2776.67Dolichol-phosphate mannose synthase subunit 3, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
Q8LEQ41.0e-2477.14Dolichol-phosphate mannose synthase subunit 3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A1S3CN504.9e-3364.38Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1C4321.5e-29100.00Dolichol-phosphate mannosyltransferase subunit 3 OS=Momordica charantia OX=3673 ... [more]
A0A6J1FU866.2e-2894.29Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1FP026.2e-2894.29Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JY891.4e-2792.86Dolichol-phosphate mannosyltransferase subunit 3 OS=Cucurbita maxima OX=3661 GN=... [more]
Match NameE-valueIdentityDescription
AT1G48140.17.4e-2677.14dolichol-phosphate mannosyltransferase-related [more]
AT1G48140.27.4e-2677.14dolichol-phosphate mannosyltransferase-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013174Dolichol-phosphate mannosyltransferase subunit 3PFAMPF08285DPM3coord: 1..69
e-value: 1.9E-14
score: 53.6
IPR013174Dolichol-phosphate mannosyltransferase subunit 3PANTHERPTHR16433DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3coord: 1..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012631.1MS012631.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006486 protein glycosylation