MS012614 (gene) Bitter gourd (TR) v1

BLAST of MS012614 vs. NCBI nr
Match: XP_022136432.1 (probable inactive serine/threonine-protein kinase bub1 [Momordica charantia])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 541/543 (99.63%), Postives = 541/543 (99.63%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSE STSNRGD
Sbjct: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSEKSTSNRGD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
           TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV
Sbjct: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP
Sbjct: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE
Sbjct: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFRED 420
           IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFRED
Sbjct: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFRED 420

Query: 421 TIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAK 480
           TIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAK
Sbjct: 421 TIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAK 480

Query: 481 PPEVHNDFGGFSILPDDDLEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP 540
           PPEV NDFGGFSILPDDDLEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP
Sbjct: 481 PPEVRNDFGGFSILPDDDLEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP 540

Query: 541 LDF 544
           LDF
Sbjct: 541 LDF 543

BLAST of MS012614 vs. NCBI nr
Match: XP_038905527.1 (mitotic spindle checkpoint protein BUBR1 [Benincasa hispida])

HSP 1 Score: 866.7 bits (2238), Expect = 1.1e-247
Identity = 448/543 (82.50%), Postives = 480/543 (88.40%), Query Frame = 0

Query: 3   MASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESD 62
           MASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSH+LN+KLPRFLQKC EKFESD
Sbjct: 1   MASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHVLNDKLPRFLQKCTEKFESD 60

Query: 63  RRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDAD 122
           RRYRDDLRYLRVWLQLMDYV DP ALLKTME KRIG K SLFYQAYALYYEKLKKFDDAD
Sbjct: 61  RRYRDDLRYLRVWLQLMDYVADPRALLKTMEEKRIGTKFSLFYQAYALYYEKLKKFDDAD 120

Query: 123 KMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTE 182
           KMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQ GRTSRRHLS+ STSN+GD E
Sbjct: 121 KMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSTSNQGDPE 180

Query: 183 ANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVVVK 242
            NN      E++DKGIF++GP+AVTL DSRNETEKMPQD A RE DE R+FKSDD +VVK
Sbjct: 181 KNNN-----EVRDKGIFENGPIAVTLTDSRNETEKMPQDSAGRETDEFRIFKSDD-MVVK 240

Query: 243 FVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHPKK 302
           FVD+A  GKS  AED  H GLVEPTVNMKEAMNAINSMF+EP+EM  +GR  S  SH KK
Sbjct: 241 FVDTAFCGKS-VAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMPPIGR-RSRGSHSKK 300

Query: 303 DSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGEIG 362
           D  LNGGFEVFVDENLD K ELEKQ+  T+P HGSI++P Q EQPP MIFVDDEAGGEIG
Sbjct: 301 DCYLNGGFEVFVDENLDRKIELEKQKGCTMPQHGSITEPHQLEQPPLMIFVDDEAGGEIG 360

Query: 363 NTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV-DDPDIQDPIQAKFREDT 422
           NTS+EKDGSK SESRD VGGST SAS + AFVFPCV+N+E++ DD D+QDPI+AKFREDT
Sbjct: 361 NTSFEKDGSKLSESRDLVGGSTASASCSKAFVFPCVDNVENIDDDSDVQDPIEAKFREDT 420

Query: 423 IVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAKP 482
           IVCRFVG AIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVRT RSKKH KP
Sbjct: 421 IVCRFVGNAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHEKP 480

Query: 483 PEVHNDFGGFSILPDDD-LEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP 542
           PEV NDFGGFSILPDD+ L P QA+PPVKSS KS + DLFEPTVFTKEAMDDINKMFGMP
Sbjct: 481 PEVQNDFGGFSILPDDELLGPAQAKPPVKSSRKSSDCDLFEPTVFTKEAMDDINKMFGMP 535

Query: 543 LDF 544
           LDF
Sbjct: 541 LDF 535

BLAST of MS012614 vs. NCBI nr
Match: XP_008460356.1 (PREDICTED: uncharacterized protein LOC103499203 isoform X1 [Cucumis melo] >XP_016902550.1 PREDICTED: uncharacterized protein LOC103499203 isoform X1 [Cucumis melo] >TYK19168.1 Mad3_BUB1_I domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-236
Identity = 431/545 (79.08%), Postives = 472/545 (86.61%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLPSH+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME K+IG KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLA+PIDELQRSY+QFLHRMKRHNYKRVQHQ GRTSRRHLS+ S SN+ D
Sbjct: 121 ADKMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
            E NN      ++Q+  IF++GPLA TL D+R+ETEK+PQD   RE DESRM KSDDT+V
Sbjct: 181 AEKNN-----IKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           V+FVD AIYGKS  AED  H GLVEPTVNMKEAMN INSMF+EP+EM  +GR  S  SH 
Sbjct: 241 VRFVDRAIYGKS-VAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGR-RSCGSHS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKD NLNGGFEVFVDE  D + ELEKQ+  T+P HGSIS+P + EQP  MIFVDDE+GGE
Sbjct: 301 KKDCNLNGGFEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV-DDPDIQDPIQAKFRE 420
           IGNTS EK G K S+SRD VGGSTTSASR+ AFVFPCV+NLE++ DD D QDP+QAKFRE
Sbjct: 361 IGNTSIEKVGLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDDDSDAQDPMQAKFRE 420

Query: 421 DTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHA 480
           DTIVCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVRT RSKKH 
Sbjct: 421 DTIVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHE 480

Query: 481 KPPEVHNDFGGFSILPDDD-LEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFG 540
           KPPEV N+FGGFSILPDD+ LEP QAQ       KS++ DLFEPTVFTKEAMDDINKMFG
Sbjct: 481 KPPEVQNNFGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFG 531

Query: 541 MPLDF 544
           MPL+F
Sbjct: 541 MPLEF 531

BLAST of MS012614 vs. NCBI nr
Match: KAA0053578.1 (Mad3_BUB1_I domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 828.9 bits (2140), Expect = 2.5e-236
Identity = 430/545 (78.90%), Postives = 472/545 (86.61%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLPSH+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME K+IG KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLA+PIDELQRSY+QFLHRMKRHNYKRVQHQ GRTSRRHLS+ S SN+ D
Sbjct: 121 ADKMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
            E NN      ++Q+  IF++GPLA TL D+R+ETEK+PQD   RE DESRM KSDDT+V
Sbjct: 181 AEKNN-----IKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           V+FVD AIYGKS  AED  H GLVEPTVNMKEAMN INSMF+EP+EM  +GR  S  SH 
Sbjct: 241 VRFVDRAIYGKS-VAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGR-RSCGSHS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKD NLNGGFEVFVDE  D + ELEKQ+  T+P HGSIS+P + EQP  MIFVDDE+GGE
Sbjct: 301 KKDCNLNGGFEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV-DDPDIQDPIQAKFRE 420
           IGNTS EK G K S+SRD VGGSTTSASR+ AFVFPCV+NLE++ DD D QDP+QAKFRE
Sbjct: 361 IGNTSIEKVGLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDDDSDAQDPMQAKFRE 420

Query: 421 DTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHA 480
           DTIVCRFVGTAIS+EPE+ENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVRT RSKKH 
Sbjct: 421 DTIVCRFVGTAISNEPEMENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHE 480

Query: 481 KPPEVHNDFGGFSILPDDD-LEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFG 540
           KPPEV N+FGGFSILPDD+ LEP QAQ       KS++ DLFEPTVFTKEAMDDINKMFG
Sbjct: 481 KPPEVQNNFGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFG 531

Query: 541 MPLDF 544
           MPL+F
Sbjct: 541 MPLEF 531

BLAST of MS012614 vs. NCBI nr
Match: XP_023552017.1 (uncharacterized protein LOC111809814 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 821.6 bits (2121), Expect = 3.9e-234
Identity = 433/545 (79.45%), Postives = 464/545 (85.14%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMA NDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLP+H+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME KRIG+KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLAEPIDELQRSYEQFL RMK+HNYK+VQHQ G+TSRRHLS+  TSN+GD
Sbjct: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKITSNQGD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
              NN      E Q+KGIFQ+G            TEKMPQD     A ESRM KSDDTVV
Sbjct: 181 AVVNNN-----EDQNKGIFQNG------------TEKMPQD----SAAESRMLKSDDTVV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
            KFVD+AIYGKS  AED  H GLVEPTVNMKEAMNAINSMF+EP+EMA VGR  SHRSH 
Sbjct: 241 AKFVDNAIYGKS-VAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMAPVGR-RSHRSHS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
             D NLNGGFEVFVDENLD K ELEKQ    +P HGS+++P Q EQPP MIFVDDEAGGE
Sbjct: 301 NNDCNLNGGFEVFVDENLDKKPELEKQ----MPQHGSMTEPCQLEQPPLMIFVDDEAGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV--DDPDIQDPIQAKFR 420
           +GNTSYEKDGSK SESR  VG S  SASR+ AFVFPCV+N E+V  DD D+QDPI+AKFR
Sbjct: 361 LGNTSYEKDGSKLSESRYVVGSSMASASRSKAFVFPCVDNPENVDDDDSDVQDPIEAKFR 420

Query: 421 EDTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKH 480
           EDT+VCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVR+ RSKKH
Sbjct: 421 EDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRSRRSKKH 480

Query: 481 AKPPEVHNDFGGFSILPDDDLE-PVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMF 540
             PPEV NDFGGFSIL DDDLE P QAQPPVKSS K ++ DLFEPTVFTKEAMDDINKMF
Sbjct: 481 ENPPEVQNDFGGFSILVDDDLEQPAQAQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMF 518

Query: 541 GMPLD 543
           GMPLD
Sbjct: 541 GMPLD 518

BLAST of MS012614 vs. ExPASy Swiss-Prot
Match: Q54CV5 (Probable inactive serine/threonine-protein kinase bub1 OS=Dictyostelium discoideum OX=44689 GN=bub1 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.7e-20
Identity = 82/319 (25.71%), Postives = 146/319 (45.77%), Query Frame = 0

Query: 16  IKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLRYLRVW 75
           I  Y G+DP+  WL+ I+ ++ S P   + E+L   L++C   F S  +Y++D RYLR+W
Sbjct: 51  INEYQGEDPIDNWLKYIKWVQQSYPGGNMKEQLIVLLERCTRLFLSTEKYKNDPRYLRIW 110

Query: 76  LQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQKLAEP 135
           +   D   DP  +   +E +RIG   SL Y+A A+ YE    ++ ADK +  G+++ A+P
Sbjct: 111 ITYADMCRDPIEVFSFLEVQRIGFYLSLLYEARAIVYENKGNYEQADKSFKQGIERKAQP 170

Query: 136 IDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNTSIIRAEI-- 195
           I+ LQ+ +  F  R+      + QHQQ +  ++  ++     + D E  NT + R+ +  
Sbjct: 171 IERLQQKHLDFERRLIARLKHQQQHQQLQQQQQQQNDQLEQQQLD-ENGNTKLTRSALGT 230

Query: 196 -------------QDKGIFQDGPLAVTLIDSRNET-EKMPQDVAEREADESRMFKSDDTV 255
                        Q KG+    PL  + ID + +       ++  +  D+    K   T 
Sbjct: 231 ISSSSVHSERKSQQTKGL----PLGNSTIDKKRKVGNDQKNNLGFQIFDDE---KGGSTT 290

Query: 256 VVKFVDSAIYGKSEAAEDPRHLGLVEPTVNMK-EAMNAINSMFAEPLEMASVGRGGSHRS 315
                DS  +G S  A      G  + T  MK + +    +   E  +++   +    + 
Sbjct: 291 NEVLTDSKFFGSSRKAAALMAAGQTQQTSQMKWDELEPELNKHKENTQLSQ--KWSDVKL 350

Query: 316 HPKKDSNLNGGFEVFVDEN 318
             KK      GF+++ D++
Sbjct: 351 LQKKQKITTAGFQIYCDQD 359

BLAST of MS012614 vs. ExPASy Swiss-Prot
Match: O22806 (Mitotic spindle checkpoint protein BUBR1 OS=Arabidopsis thaliana OX=3702 GN=BUBR1 PE=1 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 1.8e-19
Identity = 57/186 (30.65%), Postives = 100/186 (53.76%), Query Frame = 0

Query: 11  SLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLR 70
           +LI  I  Y G DPL PW+  I+ ++++ P       L    ++CV KF    RY+DDLR
Sbjct: 68  NLIEAIDEYEGDDPLSPWIECIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLR 127

Query: 71  YLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQ 130
           YL+VWL+  ++  D   + K +E   IG   +++Y AYAL+ E   K   A+++++LG+ 
Sbjct: 128 YLKVWLEYAEHCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGIS 187

Query: 131 KLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNTSIIR 190
           + A+P+++L  +Y++F+ R  R +    +  +        S  +  +RGD  A   ++  
Sbjct: 188 RDAKPVEKLNDAYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGS 247

Query: 191 AEIQDK 197
           +  Q K
Sbjct: 248 SNPQAK 253

BLAST of MS012614 vs. ExPASy Swiss-Prot
Match: O59767 (Mitotic spindle checkpoint component mad3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mad3 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.6e-18
Identity = 48/133 (36.09%), Postives = 75/133 (56.39%), Query Frame = 0

Query: 18  SYSGKDPLLPWLRGIRKMKDSLP-SHLLNEKLPRFLQKCVEKFESDRRYRDDLRYLRVWL 77
           S S  DPL  W+  I+   D+ P        L   L++C  +F  +  Y+DD+RYLR+W+
Sbjct: 65  SESLDDPLQVWIDYIKWTLDNFPQGETKTSGLVTLLERCTREFVRNPLYKDDVRYLRIWM 124

Query: 78  QLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQKLAEPI 137
           Q ++Y+++P  L   +    IG +SS+FY+ YA Y+E    F  AD++Y  G +  A+P 
Sbjct: 125 QYVNYIDEPVELFSFLAHHHIGQESSIFYEEYANYFESRGLFQKADEVYQKGKRMKAKPF 184

Query: 138 DELQRSYEQFLHR 150
              Q+ Y+QF HR
Sbjct: 185 LRFQQKYQQFTHR 197

BLAST of MS012614 vs. ExPASy Swiss-Prot
Match: Q9Z1S0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 beta OS=Mus musculus OX=10090 GN=Bub1b PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-14
Identity = 50/184 (27.17%), Postives = 94/184 (51.09%), Query Frame = 0

Query: 14  SDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLRYLR 73
           S+I+ YSG DPL  W R I   + + P       +   +++ +E  + + RY +D R+L 
Sbjct: 58  SEIRFYSGDDPLDVWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLS 117

Query: 74  VWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQKLA 133
           +W++L    N+P  +   ++++ IG   + FY ++A  YE  + F  AD ++  G+++ A
Sbjct: 118 LWIKLGHLCNEPLDMYSYLQSQGIGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKA 177

Query: 134 EPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNTSIIRAEI 193
           EP+D LQ  + QF  R+ R  +  + +++        SE   S+  + ++    + RA I
Sbjct: 178 EPLDRLQSQHRQFQSRVSRQAFLALGNEEEEALEP--SEPQRSSLAELKSRGKKMARAPI 237

Query: 194 QDKG 198
              G
Sbjct: 238 SRVG 239

BLAST of MS012614 vs. ExPASy Swiss-Prot
Match: O60566 (Mitotic checkpoint serine/threonine-protein kinase BUB1 beta OS=Homo sapiens OX=9606 GN=BUB1B PE=1 SV=3)

HSP 1 Score: 81.6 bits (200), Expect = 2.9e-14
Identity = 44/148 (29.73%), Postives = 78/148 (52.70%), Query Frame = 0

Query: 15  DIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLRYLRV 74
           +I+ Y+G DPL  W R I   + + P       +   L++ VE  + ++RY  D R+L +
Sbjct: 65  EIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNL 124

Query: 75  WLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQKLAE 134
           WL+L    N+P  +   +  + IG   + FY ++A  YE  + F  AD ++  G+Q+ AE
Sbjct: 125 WLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAE 184

Query: 135 PIDELQRSYEQFLHRMKRHNYKRVQHQQ 163
           P++ LQ  + QF  R+ R     ++ ++
Sbjct: 185 PLERLQSQHRQFQARVSRQTLLALEKEE 212

BLAST of MS012614 vs. ExPASy TrEMBL
Match: A0A6J1C5H4 (probable inactive serine/threonine-protein kinase bub1 OS=Momordica charantia OX=3673 GN=LOC111008141 PE=4 SV=1)

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 541/543 (99.63%), Postives = 541/543 (99.63%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSE STSNRGD
Sbjct: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSEKSTSNRGD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
           TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV
Sbjct: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP
Sbjct: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE
Sbjct: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFRED 420
           IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFRED
Sbjct: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFRED 420

Query: 421 TIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAK 480
           TIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAK
Sbjct: 421 TIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAK 480

Query: 481 PPEVHNDFGGFSILPDDDLEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP 540
           PPEV NDFGGFSILPDDDLEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP
Sbjct: 481 PPEVRNDFGGFSILPDDDLEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFGMP 540

Query: 541 LDF 544
           LDF
Sbjct: 541 LDF 543

BLAST of MS012614 vs. ExPASy TrEMBL
Match: A0A1S3CCD9 (uncharacterized protein LOC103499203 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499203 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.4e-237
Identity = 431/545 (79.08%), Postives = 472/545 (86.61%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLPSH+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME K+IG KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLA+PIDELQRSY+QFLHRMKRHNYKRVQHQ GRTSRRHLS+ S SN+ D
Sbjct: 121 ADKMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
            E NN      ++Q+  IF++GPLA TL D+R+ETEK+PQD   RE DESRM KSDDT+V
Sbjct: 181 AEKNN-----IKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           V+FVD AIYGKS  AED  H GLVEPTVNMKEAMN INSMF+EP+EM  +GR  S  SH 
Sbjct: 241 VRFVDRAIYGKS-VAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGR-RSCGSHS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKD NLNGGFEVFVDE  D + ELEKQ+  T+P HGSIS+P + EQP  MIFVDDE+GGE
Sbjct: 301 KKDCNLNGGFEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV-DDPDIQDPIQAKFRE 420
           IGNTS EK G K S+SRD VGGSTTSASR+ AFVFPCV+NLE++ DD D QDP+QAKFRE
Sbjct: 361 IGNTSIEKVGLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDDDSDAQDPMQAKFRE 420

Query: 421 DTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHA 480
           DTIVCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVRT RSKKH 
Sbjct: 421 DTIVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHE 480

Query: 481 KPPEVHNDFGGFSILPDDD-LEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFG 540
           KPPEV N+FGGFSILPDD+ LEP QAQ       KS++ DLFEPTVFTKEAMDDINKMFG
Sbjct: 481 KPPEVQNNFGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFG 531

Query: 541 MPLDF 544
           MPL+F
Sbjct: 541 MPLEF 531

BLAST of MS012614 vs. ExPASy TrEMBL
Match: A0A5D3D6J0 (Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00980 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.4e-237
Identity = 431/545 (79.08%), Postives = 472/545 (86.61%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLPSH+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME K+IG KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLA+PIDELQRSY+QFLHRMKRHNYKRVQHQ GRTSRRHLS+ S SN+ D
Sbjct: 121 ADKMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
            E NN      ++Q+  IF++GPLA TL D+R+ETEK+PQD   RE DESRM KSDDT+V
Sbjct: 181 AEKNN-----IKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           V+FVD AIYGKS  AED  H GLVEPTVNMKEAMN INSMF+EP+EM  +GR  S  SH 
Sbjct: 241 VRFVDRAIYGKS-VAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGR-RSCGSHS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKD NLNGGFEVFVDE  D + ELEKQ+  T+P HGSIS+P + EQP  MIFVDDE+GGE
Sbjct: 301 KKDCNLNGGFEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV-DDPDIQDPIQAKFRE 420
           IGNTS EK G K S+SRD VGGSTTSASR+ AFVFPCV+NLE++ DD D QDP+QAKFRE
Sbjct: 361 IGNTSIEKVGLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDDDSDAQDPMQAKFRE 420

Query: 421 DTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHA 480
           DTIVCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVRT RSKKH 
Sbjct: 421 DTIVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHE 480

Query: 481 KPPEVHNDFGGFSILPDDD-LEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFG 540
           KPPEV N+FGGFSILPDD+ LEP QAQ       KS++ DLFEPTVFTKEAMDDINKMFG
Sbjct: 481 KPPEVQNNFGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFG 531

Query: 541 MPLDF 544
           MPL+F
Sbjct: 541 MPLEF 531

BLAST of MS012614 vs. ExPASy TrEMBL
Match: A0A5A7UEU2 (Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G001480 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.2e-236
Identity = 430/545 (78.90%), Postives = 472/545 (86.61%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLPSH+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME K+IG KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           ADKMYHLGVQKLA+PIDELQRSY+QFLHRMKRHNYKRVQHQ GRTSRRHLS+ S SN+ D
Sbjct: 121 ADKMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
            E NN      ++Q+  IF++GPLA TL D+R+ETEK+PQD   RE DESRM KSDDT+V
Sbjct: 181 AEKNN-----IKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
           V+FVD AIYGKS  AED  H GLVEPTVNMKEAMN INSMF+EP+EM  +GR  S  SH 
Sbjct: 241 VRFVDRAIYGKS-VAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGR-RSCGSHS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKD NLNGGFEVFVDE  D + ELEKQ+  T+P HGSIS+P + EQP  MIFVDDE+GGE
Sbjct: 301 KKDCNLNGGFEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV-DDPDIQDPIQAKFRE 420
           IGNTS EK G K S+SRD VGGSTTSASR+ AFVFPCV+NLE++ DD D QDP+QAKFRE
Sbjct: 361 IGNTSIEKVGLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENMDDDSDAQDPMQAKFRE 420

Query: 421 DTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHA 480
           DTIVCRFVGTAIS+EPE+ENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVRT RSKKH 
Sbjct: 421 DTIVCRFVGTAISNEPEMENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHE 480

Query: 481 KPPEVHNDFGGFSILPDDD-LEPVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMFG 540
           KPPEV N+FGGFSILPDD+ LEP QAQ       KS++ DLFEPTVFTKEAMDDINKMFG
Sbjct: 481 KPPEVQNNFGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFG 531

Query: 541 MPLDF 544
           MPL+F
Sbjct: 541 MPLEF 531

BLAST of MS012614 vs. ExPASy TrEMBL
Match: A0A6J1ERG0 (uncharacterized protein LOC111435869 OS=Cucurbita moschata OX=3662 GN=LOC111435869 PE=4 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 3.3e-234
Identity = 430/545 (78.90%), Postives = 467/545 (85.69%), Query Frame = 0

Query: 1   MAMASNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFE 60
           MAMA NDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLP+H+LN+KLPRFLQKC EKFE
Sbjct: 1   MAMAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFE 60

Query: 61  SDRRYRDDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDD 120
           SDRRYRDDLRYLRVWLQLMDYV+DP ALLKTME KRIG+KSSLFYQAYALYYEKLKKFDD
Sbjct: 61  SDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDD 120

Query: 121 ADKMYHLGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGD 180
           A+KMYHLGVQKLAEPIDELQRSYEQFL RMK+HNYK+VQHQ G+TSRRHLS+ STSN+GD
Sbjct: 121 AEKMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTSNQGD 180

Query: 181 TEANNTSIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVV 240
              NN      E Q+KGIFQ+G            TEKMPQD     A E+RM +SDDTVV
Sbjct: 181 AVVNNN-----EDQNKGIFQNG------------TEKMPQD----SAAEARMLRSDDTVV 240

Query: 241 VKFVDSAIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHP 300
            KFVD+AIYGKS  AED  H GLVEPTVNMKEAMNAINSMF+EP+EMA +GR  SHRS+ 
Sbjct: 241 AKFVDNAIYGKS-VAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMAPIGR-RSHRSYS 300

Query: 301 KKDSNLNGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGE 360
           KKD NLNGGFEVFVDENLD K ELEKQ    +P HGS+++P Q EQPP MIFVDDE GGE
Sbjct: 301 KKDCNLNGGFEVFVDENLDKKPELEKQ----MPQHGSMTEPCQLEQPPLMIFVDDEVGGE 360

Query: 361 IGNTSYEKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDV--DDPDIQDPIQAKFR 420
           +GNTSYEKDGSK S+SR  VG ST SASR+ AFVFPCV+N E+V  DD D+QDPI+AKFR
Sbjct: 361 LGNTSYEKDGSKLSDSRYVVGSSTASASRSKAFVFPCVDNPENVNDDDSDVQDPIEAKFR 420

Query: 421 EDTIVCRFVGTAISHEPEVENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKH 480
           EDT+VCRFVGTAIS+EPEVENICHHGLVEPTVNLKEAMDDINNMFG+PIEFVR+ RSKKH
Sbjct: 421 EDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRSRRSKKH 480

Query: 481 AKPPEVHNDFGGFSILPDDDLE-PVQAQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMF 540
             PPEV NDFGGFSIL DDDLE P QAQPPVKSS K ++ DLFEPTVFTKEAMDDINKMF
Sbjct: 481 ENPPEVQNDFGGFSILVDDDLEQPAQAQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMF 518

Query: 541 GMPLD 543
           GMPLD
Sbjct: 541 GMPLD 518

BLAST of MS012614 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 461.8 bits (1187), Expect = 7.4e-130
Identity = 271/546 (49.63%), Postives = 336/546 (61.54%), Query Frame = 0

Query: 7   DLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYR 66
           +L +SLI DIKSYSGKD LLPW+RG++KMK+SLPS +LNEKLPRFLQKC E FESD+RY+
Sbjct: 10  NLLASLIVDIKSYSGKDHLLPWIRGVKKMKESLPSQILNEKLPRFLQKCAESFESDKRYK 69

Query: 67  DDLRYLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYH 126
           +D RY+RVWLQLMD+V+DP ALL+TMEAK IG K SLFYQAYAL+YEK+K+F+DA+KMY 
Sbjct: 70  NDSRYIRVWLQLMDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYR 129

Query: 127 LGVQKLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNT 186
           LGVQ LAEP+DELQ+SY QF+ RM+RH  K+ Q Q+ + S +H                 
Sbjct: 130 LGVQNLAEPMDELQKSYLQFVTRMERHKKKKTQRQEQKLSGKH----------------- 189

Query: 187 SIIRAEIQDKGIFQDGPLAVTLIDSRNETEKMPQDVAEREADESRMFKSDDTVVVKFVDS 246
                                              V ER+  E          V+ FVD 
Sbjct: 190 ---------------------------------HKVDERQQHEP---------VLNFVDK 249

Query: 247 AIYGKSEAAEDPRHLGLVEPTVNMKEAMNAINSMFAEPLEMASVGRGGSHRSHPKKDSNL 306
           AI GK E AE+  H GLV+PT+NMKEAMN IN+MF EP+E A + R    RS  K++   
Sbjct: 250 AIVGKPE-AENACHHGLVDPTINMKEAMNTINNMFKEPIETAPLQRRSRQRSQNKENQGC 309

Query: 307 NGGFEVFVDENLDNKTELEKQERSTVPHHGSISQPRQPEQPPFMIFVDDEAGGEIGNTSY 366
           N  FEVF+DENL+ +T    + +++    GS     QP Q  F IF+DDE   E   T+ 
Sbjct: 310 NNSFEVFLDENLECETGTSGKAKTSTTQCGS-----QPNQESFEIFIDDENTDE---TAD 369

Query: 367 EKDGSKSSESRDFVGGSTTSASRANAFVFPCVENLEDVDDPDIQDPIQAKFREDTIVCRF 426
           E D                 A +A  F+ P   + E  ++ D   P +A+FREDT+V RF
Sbjct: 370 END----------------EAGKAFVFLLPRDHSPESSEEADRNTPPRARFREDTVVRRF 429

Query: 427 VGTAISHEPE-VENICHHGLVEPTVNLKEAMDDINNMFGEPIEFVRTGRSKKHAKP--PE 486
           VG+ IS EPE VEN CHHGLV+PTVNLKEAM+DINNMFGEPI FVR  RSK   K     
Sbjct: 430 VGSTISDEPEAVENACHHGLVDPTVNLKEAMEDINNMFGEPINFVRPNRSKNKGKAVVET 471

Query: 487 VHNDFGGFSILPDDDLEPVQ------AQPPVKSSGKSKERDLFEPTVFTKEAMDDINKMF 544
             N   GFSIL DDD E  +      +QP  +   KS ER+LFEPTV TK A+D+INK+F
Sbjct: 490 KPNPAAGFSILEDDDEEEAEQEHQGKSQPTQRLPSKSDERELFEPTVCTKVALDEINKLF 471

BLAST of MS012614 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 100.5 bits (249), Expect = 4.3e-21
Identity = 60/187 (32.09%), Postives = 102/187 (54.55%), Query Frame = 0

Query: 11  SLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLR 70
           +LI  I  Y G DPL PW+  I+ ++++ P       L    ++CV KF    RY+DDLR
Sbjct: 68  NLIEAIDEYEGDDPLSPWIECIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLR 127

Query: 71  YLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQ 130
           YL+VWL+  ++  D   + K +E   IG   +++Y AYAL+ E   K   A+++++LG+ 
Sbjct: 128 YLKVWLEYAEHCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGIS 187

Query: 131 KLAEPIDELQRSYEQFLHR-MKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNTSII 190
           + A+P+++L  +Y++F+ R M+R N    Q  +        S  +  +RGD  A   ++ 
Sbjct: 188 RDAKPVEKLNDAYKKFMVRTMRRSNTADEQEPKENNDLPSRSFGTLLSRGDNNARRQALG 247

Query: 191 RAEIQDK 197
            +  Q K
Sbjct: 248 SSNPQAK 254

BLAST of MS012614 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 99.0 bits (245), Expect = 1.3e-20
Identity = 57/186 (30.65%), Postives = 100/186 (53.76%), Query Frame = 0

Query: 11  SLISDIKSYSGKDPLLPWLRGIRKMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLR 70
           +LI  I  Y G DPL PW+  I+ ++++ P       L    ++CV KF    RY+DDLR
Sbjct: 68  NLIEAIDEYEGDDPLSPWIECIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLR 127

Query: 71  YLRVWLQLMDYVNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQ 130
           YL+VWL+  ++  D   + K +E   IG   +++Y AYAL+ E   K   A+++++LG+ 
Sbjct: 128 YLKVWLEYAEHCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGIS 187

Query: 131 KLAEPIDELQRSYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNTSIIR 190
           + A+P+++L  +Y++F+ R  R +    +  +        S  +  +RGD  A   ++  
Sbjct: 188 RDAKPVEKLNDAYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGS 247

Query: 191 AEIQDK 197
           +  Q K
Sbjct: 248 SNPQAK 253

BLAST of MS012614 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 73.2 bits (178), Expect = 7.4e-13
Identity = 49/173 (28.32%), Postives = 83/173 (47.98%), Query Frame = 0

Query: 23  DPLLPWLRGIR-KMKDSLPSHLLNEKLPRFLQKCVEKFESDRRYRDDLRYLRVWLQLMDY 82
           DPL PWL  I+  M+D          L + L  C+  ++ D RYR+DLR+L++W   ++ 
Sbjct: 11  DPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEG 70

Query: 83  VNDPNALLKTMEAKRIGAKSSLFYQAYALYYEKLKKFDDADKMYHLGVQKLAEPIDELQR 142
             D   + + +E   I    SL Y+ YA++ E    +  A+ +Y  G+ + AEP D L+ 
Sbjct: 71  REDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKE 130

Query: 143 SYEQFLHRMKRHNYKRVQHQQGRTSRRHLSETSTSNRGDTEANNTSIIRAEIQ 195
           ++  FL R+ +        + G  ++    ET   N  +T   N  I + + Q
Sbjct: 131 AHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQ 183