MS012578 (gene) Bitter gourd (TR) v1

BLAST of MS012578 vs. NCBI nr
Match: XP_022136725.1 (mechanosensitive ion channel protein 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 681/683 (99.71%), Postives = 681/683 (99.71%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG
Sbjct: 49  QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 108

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW
Sbjct: 109 CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 168

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
           AGV LICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE
Sbjct: 169 AGVALICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 228

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 229 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 288

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI
Sbjct: 289 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 348

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 349 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 408

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL
Sbjct: 409 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 468

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 469 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 528

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI
Sbjct: 529 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 588

Query: 541 SGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSAT 600
           SGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSAT
Sbjct: 589 SGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSAT 648

Query: 601 SQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWN 660
           SQPRIEGEQTPV KPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWN
Sbjct: 649 SQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWN 708

Query: 661 GNGTNGATTPDKDTKDQTPPPSE 684
           GNGTNGATTPDKDTKDQTPPPSE
Sbjct: 709 GNGTNGATTPDKDTKDQTPPPSE 731

BLAST of MS012578 vs. NCBI nr
Match: KAG6577342.1 (Mechanosensitive ion channel protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 583/687 (84.86%), Postives = 629/687 (91.56%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 49  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 108

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW
Sbjct: 109 CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLW 168

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 169 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 228

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 229 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 288

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 289 FLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 348

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 349 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 408

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 409 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 468

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 469 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGD 528

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           +KAAKE TPS+RKTEV+TGE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI
Sbjct: 529 NKAAKETTPSERKTEVKTGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGI 588

Query: 541 SGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGS 600
           + MP+SDAKTT SD DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +
Sbjct: 589 ADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTT 648

Query: 601 SATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKD 660
           S+ SQPRIEG+QT  + PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KD
Sbjct: 649 SSASQPRIEGDQT--TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKD 708

Query: 661 L-AARWNGNGTNGATTPDKDTKDQTPP 681
           L AA  NGNG NGATTPDK+TK Q+PP
Sbjct: 709 LAAASLNGNGANGATTPDKETKRQSPP 731

BLAST of MS012578 vs. NCBI nr
Match: XP_022929456.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 580/687 (84.43%), Postives = 626/687 (91.12%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 29  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 88

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW
Sbjct: 89  CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLW 148

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 149 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 208

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 209 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 268

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 269 FLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 328

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 329 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 388

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 389 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 448

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 449 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGD 508

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           +KAAKE TPS+RKTEV+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI
Sbjct: 509 NKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGI 568

Query: 541 SGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGS 600
           + MP+SDAKTT SD DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +
Sbjct: 569 ADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTT 628

Query: 601 SATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKD 660
           S+ SQPRIEG+QT  + PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KD
Sbjct: 629 SSASQPRIEGDQT--TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKD 688

Query: 661 L-AARWNGNGTNGATTPDKDTKDQTPP 681
           L AA  NGNG  GATTPDK+TK  +PP
Sbjct: 689 LAAASLNGNGATGATTPDKETKRLSPP 711

BLAST of MS012578 vs. NCBI nr
Match: XP_022929450.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929451.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929452.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929453.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929454.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929455.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 580/687 (84.43%), Postives = 626/687 (91.12%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 49  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 108

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW
Sbjct: 109 CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLW 168

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 169 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 228

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 229 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 288

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 289 FLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 348

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 349 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 408

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 409 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 468

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 469 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGD 528

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           +KAAKE TPS+RKTEV+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI
Sbjct: 529 NKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGI 588

Query: 541 SGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGS 600
           + MP+SDAKTT SD DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +
Sbjct: 589 ADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTT 648

Query: 601 SATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKD 660
           S+ SQPRIEG+QT  + PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KD
Sbjct: 649 SSASQPRIEGDQT--TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKD 708

Query: 661 L-AARWNGNGTNGATTPDKDTKDQTPP 681
           L AA  NGNG  GATTPDK+TK  +PP
Sbjct: 709 LAAASLNGNGATGATTPDKETKRLSPP 731

BLAST of MS012578 vs. NCBI nr
Match: XP_023552801.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 578/688 (84.01%), Postives = 629/688 (91.42%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDPPG+KAA+VALTRFCNVLGG
Sbjct: 29  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPPGMKAAIVALTRFCNVLGG 88

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++F+VWG+GPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW
Sbjct: 89  CPPPVVKMVPAVSIMIFSVWGIGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLW 148

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 149 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 208

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 209 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 268

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 269 FLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 328

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 329 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 388

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 389 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 448

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSR-TTGEQNGKPIARSSG 480
           ES+PFSDSIFG GG  LNRRMLMIEPPYK+YG+DRKQSH RTSR TTGEQN KPIARSSG
Sbjct: 449 ESIPFSDSIFGHGGATLNRRMLMIEPPYKIYGDDRKQSHGRTSRTTTGEQNSKPIARSSG 508

Query: 481 DSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNG 540
           D+KA+KE TPS+RKTEV+TGE +DSDTK H KVP S S+D  S+ESKHKSSSRS SSTNG
Sbjct: 509 DNKASKETTPSERKTEVKTGEGRDSDTKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNG 568

Query: 541 ISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGG 600
           I+ MPSSDAKTT SDTDNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G 
Sbjct: 569 IADMPSSDAKTTTSDTDNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGT 628

Query: 601 SSATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VK 660
           +S+ SQPRIEG+QT  + PST KPGVEEN+ILGVALDGSKRTLPI++D PSSP+T    K
Sbjct: 629 TSSASQPRIEGDQT--TNPSTTKPGVEENIILGVALDGSKRTLPIDDDTPSSPQTTLGAK 688

Query: 661 DL-AARWNGNGTNGATTPDKDTKDQTPP 681
           DL AA  NGNG  GATTPDK+TK Q+PP
Sbjct: 689 DLAAASLNGNGATGATTPDKETKRQSPP 712

BLAST of MS012578 vs. ExPASy Swiss-Prot
Match: Q56X46 (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL2 PE=2 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 3.9e-197
Identity = 386/668 (57.78%), Postives = 468/668 (70.06%), Query Frame = 0

Query: 16  PNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCII 75
           P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 63  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 122

Query: 76  MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVTLICRALDPVVL 135
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 123 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 182

Query: 136 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 195
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 183 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 242

Query: 196 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 255
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 243 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 302

Query: 256 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 315
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 303 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 362

Query: 316 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 375
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 363 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 422

Query: 376 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 435
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 423 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 482

Query: 436 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 495
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 483 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 542

Query: 496 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 555
           V+ GE   SDT K P+                                     +T     
Sbjct: 543 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 602

Query: 556 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVSKP 615
             +   P T +    SG+ K                K++GG+  +++     ++T  S  
Sbjct: 603 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKT----DETDSSTS 662

Query: 616 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 675
           S  +  +EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 663 SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 666

Query: 676 KDQTPPPS 683
           KD    P+
Sbjct: 723 KDSQSQPN 666

BLAST of MS012578 vs. ExPASy Swiss-Prot
Match: Q8L7W1 (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL3 PE=1 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 2.3e-173
Identity = 352/656 (53.66%), Postives = 443/656 (67.53%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           +++ WS  L          V  R N F C S+L      + P +K+  V  TR  + LGG
Sbjct: 48  RQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG 107

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLL 120
             P ++KL+PAV I+ FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLL
Sbjct: 108 -NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLL 167

Query: 121 WAGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETT 180
           W+G  L+CR LDP+VLP+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET 
Sbjct: 168 WSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETN 227

Query: 181 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 240
             +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI T
Sbjct: 228 NPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILT 287

Query: 241 NFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFT 300
           NFLSS+MIHATRPF++NEWIQTKI GYEVSGTVE VGWWSPTIIRG+DREAVHIPNHQF+
Sbjct: 288 NFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFS 347

Query: 301 INVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 360
           +N+VRNL+QKTHWRIKTHLAISHLDV+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE++
Sbjct: 348 VNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDI 407

Query: 361 NPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSD 420
           +PENQAL ILISCFVKTS FEEYLCVKEA++LDLL VIRHH ARLATPIRT+Q+M ++++
Sbjct: 408 DPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAE 467

Query: 421 LESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSG 480
           +++  FSD +F +    +NRR ++IEP YK+  +D  +S    S + G+++  P  +S  
Sbjct: 468 VDTAGFSDIVFNQAA--MNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS-- 527

Query: 481 DSKAAKEATPSDRKTEV-RTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSS------R 540
             +   +  PS+ K E    G    S+ KK  +     S+ N+  +    S+S      +
Sbjct: 528 -EERDLQEEPSETKAETENNGSVPVSNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQK 587

Query: 541 SASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVK 600
           S        G P    K   SD + + E   T +S+   GS K N +S            
Sbjct: 588 SEEKKKESVGDPHKAEKDEVSDDEATIE--QTLKSKAKQGSEKNNGESK----------A 647

Query: 601 KTGGSSATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSS 649
           + GG S TS                    +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 648 RDGGGSGTSSL------------------LEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of MS012578 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.8e-22
Identity = 66/211 (31.28%), Postives = 116/211 (54.98%), Query Frame = 0

Query: 202 VAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQT 261
           V  V L+ E  G S    LT GG+G + + +AG++I +NF S +M++  RPF + +WI++
Sbjct: 128 VVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRS 187

Query: 262 KIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAIS 321
                E  GTV  +G W  T I   D   +++PN  F+   V N  + T+ RI T + + 
Sbjct: 188 PDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLR 247

Query: 322 HLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNP-ENQALLILISCFVKTSHFE 381
           + D  K+  IV  +R++L  +P ++Q    R+  L   N   + +L I++ CF KT+ + 
Sbjct: 248 YEDAAKVGVIVEAVREMLKNHPAIDQ----RQTLLVYFNQFADSSLNIMVYCFTKTTVWA 307

Query: 382 EYLCVKEAIILDLLRVIRHHRARLATPIRTM 412
           E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 308 EWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of MS012578 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.8e-22
Identity = 66/211 (31.28%), Postives = 116/211 (54.98%), Query Frame = 0

Query: 202 VAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQT 261
           V  V L+ E  G S    LT GG+G + + +AG++I +NF S +M++  RPF + +WI++
Sbjct: 128 VVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRS 187

Query: 262 KIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAIS 321
                E  GTV  +G W  T I   D   +++PN  F+   V N  + T+ RI T + + 
Sbjct: 188 PDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLR 247

Query: 322 HLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNP-ENQALLILISCFVKTSHFE 381
           + D  K+  IV  +R++L  +P ++Q    R+  L   N   + +L I++ CF KT+ + 
Sbjct: 248 YEDAAKVGVIVEAVREMLKNHPAIDQ----RQTLLVYFNQFADSSLNIMVYCFTKTTVWA 307

Query: 382 EYLCVKEAIILDLLRVIRHHRARLATPIRTM 412
           E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 308 EWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of MS012578 vs. ExPASy Swiss-Prot
Match: O66994 (Uncharacterized MscS family protein aq_812 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_812 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.6e-12
Identity = 64/240 (26.67%), Postives = 122/240 (50.83%), Query Frame = 0

Query: 173 KFFSETTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTL 232
           +FF +      A  +G  F  K + + V V   +  ++  G +    L + GL  + ++L
Sbjct: 130 EFFVKVGGKDFAEEVG-DFILKILKAFVVVIVGASLLQEWGVNIGAILASVGLLGLAVSL 189

Query: 233 AGREIFTNFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVH 292
           A ++ F N LS ++I   +P  V E ++ K    +  G+VE +G  S T IR  D+  V 
Sbjct: 190 AAKDTFENILSGLIILLDKPVKVGETVKVK----DFMGSVEDIGLRS-TKIRTFDKSLVT 249

Query: 293 IPNHQFTINVVRNLSQKTHWRIKTHLAISH-LDVNKINNIVADMRKVLAKNPQVEQQRLH 352
           IPN     N V N +++   R++ ++ + +     ++ NI+ ++R++L ++P V +    
Sbjct: 250 IPNRDIVNNHVENFTRRNKRRVRFYIGVVYSTKREQLENILKEIRELLKEHPGVAKDEKF 309

Query: 353 RRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 412
             V+ EN    + +L ILI  +  T+ +EEYL + E I L ++ ++  + +  A P R++
Sbjct: 310 -YVYFENYG--DSSLNILIQYYANTNDYEEYLKIIEDINLKIMEIVEKNGSSFAFPSRSV 360

BLAST of MS012578 vs. ExPASy TrEMBL
Match: A0A6J1C4S5 (mechanosensitive ion channel protein 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008365 PE=4 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 681/683 (99.71%), Postives = 681/683 (99.71%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG
Sbjct: 49  QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 108

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW
Sbjct: 109 CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 168

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
           AGV LICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE
Sbjct: 169 AGVALICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 228

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 229 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 288

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI
Sbjct: 289 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 348

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 349 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 408

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL
Sbjct: 409 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 468

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 469 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 528

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI
Sbjct: 529 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 588

Query: 541 SGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSAT 600
           SGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSAT
Sbjct: 589 SGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSAT 648

Query: 601 SQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWN 660
           SQPRIEGEQTPV KPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWN
Sbjct: 649 SQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWN 708

Query: 661 GNGTNGATTPDKDTKDQTPPPSE 684
           GNGTNGATTPDKDTKDQTPPPSE
Sbjct: 709 GNGTNGATTPDKDTKDQTPPPSE 731

BLAST of MS012578 vs. ExPASy TrEMBL
Match: A0A6J1ES61 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 580/687 (84.43%), Postives = 626/687 (91.12%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 49  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 108

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW
Sbjct: 109 CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLW 168

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 169 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 228

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 229 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 288

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 289 FLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 348

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 349 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 408

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 409 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 468

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 469 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGD 528

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           +KAAKE TPS+RKTEV+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI
Sbjct: 529 NKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGI 588

Query: 541 SGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGS 600
           + MP+SDAKTT SD DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +
Sbjct: 589 ADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTT 648

Query: 601 SATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKD 660
           S+ SQPRIEG+QT  + PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KD
Sbjct: 649 SSASQPRIEGDQT--TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKD 708

Query: 661 L-AARWNGNGTNGATTPDKDTKDQTPP 681
           L AA  NGNG  GATTPDK+TK  +PP
Sbjct: 709 LAAASLNGNGATGATTPDKETKRLSPP 731

BLAST of MS012578 vs. ExPASy TrEMBL
Match: A0A6J1EN69 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 580/687 (84.43%), Postives = 626/687 (91.12%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 29  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 88

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW
Sbjct: 89  CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLW 148

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 149 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 208

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 209 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 268

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQT+IEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 269 FLSSVMIHATRPFVVNEWIQTRIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 328

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 329 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 388

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 389 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 448

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQNGKPIARSSGD
Sbjct: 449 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNGKPIARSSGD 508

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGI 540
           +KAAKE TPS+RKTEV+ GE +DSD K H KVP S S+D  S+ESKHKSSSRS SSTNGI
Sbjct: 509 NKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTSEDKPSSESKHKSSSRSVSSTNGI 568

Query: 541 SGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGGS 600
           + MP+SDAKTT SD DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G +
Sbjct: 569 ADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGTT 628

Query: 601 SATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VKD 660
           S+ SQPRIEG+QT  + PST KPGVEEN+ILGVALDGSKRTLPIE+D PSSP+T    KD
Sbjct: 629 SSASQPRIEGDQT--TNPSTTKPGVEENIILGVALDGSKRTLPIEDDTPSSPQTTLGAKD 688

Query: 661 L-AARWNGNGTNGATTPDKDTKDQTPP 681
           L AA  NGNG  GATTPDK+TK  +PP
Sbjct: 689 LAAASLNGNGATGATTPDKETKRLSPP 711

BLAST of MS012578 vs. ExPASy TrEMBL
Match: A0A6J1JBP8 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483011 PE=4 SV=1)

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 575/688 (83.58%), Postives = 624/688 (90.70%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 49  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 108

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVM+LYLQPLLLW
Sbjct: 109 CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMSLYLQPLLLW 168

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 169 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 228

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 229 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 288

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 289 FLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 348

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 349 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 408

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 409 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 468

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQN KPIARSSGD
Sbjct: 469 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNSKPIARSSGD 528

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNS-SNESKHKSSSRSASSTNG 540
           +KAAKE +PS+RK EV+TGE +DSDTK + KVP S S+DN  S+ESKHKSSSRS SSTNG
Sbjct: 529 NKAAKETSPSERKMEVKTGEGRDSDTKIYSKVPMSTSEDNKPSSESKHKSSSRSVSSTNG 588

Query: 541 ISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGG 600
           I+ MP+SDAKTT  D DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G 
Sbjct: 589 IADMPTSDAKTTTLDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGT 648

Query: 601 SSATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VK 660
           +S+ SQPRIEG+QT  + P T KP VEEN+ILGVALD SKRTLPIE+D PSSP+T    K
Sbjct: 649 TSSASQPRIEGDQT--TNPPTTKPSVEENIILGVALDRSKRTLPIEDDTPSSPQTSLGSK 708

Query: 661 DL-AARWNGNGTNGATTPDKDTKDQTPP 681
           DL AA  NGN  +GATTPDK+TK Q+PP
Sbjct: 709 DLAAASLNGNVASGATTPDKETKRQSPP 732

BLAST of MS012578 vs. ExPASy TrEMBL
Match: A0A6J1J9R4 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483011 PE=4 SV=1)

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 575/688 (83.58%), Postives = 624/688 (90.70%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           QKNTWS+HLFS+KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGG
Sbjct: 29  QKNTWSSHLFSLKYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGG 88

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLW 120
           CPPPV+K+VPAV I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVM+LYLQPLLLW
Sbjct: 89  CPPPVVKMVPAVSIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMSLYLQPLLLW 148

Query: 121 AGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTE 180
            G TLICRALDPVVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+E
Sbjct: 149 TGATLICRALDPVVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSE 208

Query: 181 SSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 240
           SSDARNMGFQFAWKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN
Sbjct: 209 SSDARNMGFQFAWKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTN 268

Query: 241 FLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTI 300
           FLSSVMIHATRPF+VNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNH+FT+
Sbjct: 269 FLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTM 328

Query: 301 NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 360
           NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN
Sbjct: 329 NVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVN 388

Query: 361 PENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDL 420
           PENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVIRHHRARLATPIRT+QKMHSDSDL
Sbjct: 389 PENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVIRHHRARLATPIRTVQKMHSDSDL 448

Query: 421 ESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGD 480
           ESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQSHSRTSRTTGEQN KPIARSSGD
Sbjct: 449 ESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQSHSRTSRTTGEQNSKPIARSSGD 508

Query: 481 SKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNS-SNESKHKSSSRSASSTNG 540
           +KAAKE +PS+RK EV+TGE +DSDTK + KVP S S+DN  S+ESKHKSSSRS SSTNG
Sbjct: 509 NKAAKETSPSERKMEVKTGEGRDSDTKIYSKVPMSTSEDNKPSSESKHKSSSRSVSSTNG 568

Query: 541 ISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS-TKQNYKSNHPAVSLPEDVKK-TGG 600
           I+ MP+SDAKTT  D DNS +D  S+K+S+N SGS  KQNYK  H +VS  EDVKK  G 
Sbjct: 569 IADMPTSDAKTTTLDADNSMQDSGSSKKSKNSSGSNNKQNYKPIHSSVSFQEDVKKPEGT 628

Query: 601 SSATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPET---VK 660
           +S+ SQPRIEG+QT  + P T KP VEEN+ILGVALD SKRTLPIE+D PSSP+T    K
Sbjct: 629 TSSASQPRIEGDQT--TNPPTTKPSVEENIILGVALDRSKRTLPIEDDTPSSPQTSLGSK 688

Query: 661 DL-AARWNGNGTNGATTPDKDTKDQTPP 681
           DL AA  NGN  +GATTPDK+TK Q+PP
Sbjct: 689 DLAAASLNGNVASGATTPDKETKRQSPP 712

BLAST of MS012578 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 689.5 bits (1778), Expect = 2.7e-198
Identity = 386/668 (57.78%), Postives = 468/668 (70.06%), Query Frame = 0

Query: 16  PNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCII 75
           P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 63  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 122

Query: 76  MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVTLICRALDPVVL 135
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 123 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 182

Query: 136 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 195
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 183 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 242

Query: 196 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 255
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 243 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 302

Query: 256 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 315
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 303 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 362

Query: 316 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 375
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 363 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 422

Query: 376 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 435
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 423 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 482

Query: 436 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 495
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 483 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 542

Query: 496 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 555
           V+ GE   SDT K P+                                     +T     
Sbjct: 543 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 602

Query: 556 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVSKP 615
             +   P T +    SG+ K                K++GG+  +++     ++T  S  
Sbjct: 603 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKT----DETDSSTS 662

Query: 616 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 675
           S  +  +EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 663 SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 666

Query: 676 KDQTPPPS 683
           KD    P+
Sbjct: 723 KDSQSQPN 666

BLAST of MS012578 vs. TAIR 10
Match: AT5G10490.2 (MSCS-like 2 )

HSP 1 Score: 689.5 bits (1778), Expect = 2.7e-198
Identity = 386/668 (57.78%), Postives = 468/668 (70.06%), Query Frame = 0

Query: 16  PNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCII 75
           P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 35  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 94

Query: 76  MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVTLICRALDPVVL 135
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 95  VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 154

Query: 136 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 195
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 155 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 214

Query: 196 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 255
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 215 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 274

Query: 256 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 315
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 275 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 334

Query: 316 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 375
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 335 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 394

Query: 376 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 435
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 395 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 454

Query: 436 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 495
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 455 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 514

Query: 496 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 555
           V+ GE   SDT K P+                                     +T     
Sbjct: 515 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 574

Query: 556 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVSKP 615
             +   P T +    SG+ K                K++GG+  +++     ++T  S  
Sbjct: 575 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKT----DETDSSTS 634

Query: 616 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 675
           S  +  +EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 635 SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 638

Query: 676 KDQTPPPS 683
           KD    P+
Sbjct: 695 KDSQSQPN 638

BLAST of MS012578 vs. TAIR 10
Match: AT5G10490.3 (MSCS-like 2 )

HSP 1 Score: 689.5 bits (1778), Expect = 2.7e-198
Identity = 386/668 (57.78%), Postives = 468/668 (70.06%), Query Frame = 0

Query: 16  PNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCII 75
           P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 61  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 120

Query: 76  MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVTLICRALDPVVL 135
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 121 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 180

Query: 136 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 195
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 181 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 240

Query: 196 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 255
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 241 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 300

Query: 256 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIK 315
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 360

Query: 316 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVK 375
           THLAISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 420

Query: 376 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGV 435
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++  GGV
Sbjct: 421 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGV 480

Query: 436 NLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTE 495
              R +++IEP YK+ GED+ +S +R ++ T EQ  K      G +  +KE +  D K  
Sbjct: 481 TSRRPLMLIEPAYKINGEDKSKSQNRAAKPTAEQENK------GSNPKSKETSSPDLKAN 540

Query: 496 VRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDT 555
           V+ GE   SDT K P+                                     +T     
Sbjct: 541 VKVGESPVSDTNKVPE-------------------------------------ETVAKPV 600

Query: 556 DNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVSKP 615
             +   P T +    SG+ K                K++GG+  +++     ++T  S  
Sbjct: 601 IKAVSKPPTPKDTETSGTEKPK-------------AKRSGGTIKSTKT----DETDSSTS 660

Query: 616 STLKPGVEENLILGVALDGSKRTLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDT 675
           S  +  +EEN++LGVAL+GSKRTLPIEE++ S P ET          +G NG    DK+ 
Sbjct: 661 SASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQ 664

Query: 676 KDQTPPPS 683
           KD    P+
Sbjct: 721 KDSQSQPN 664

BLAST of MS012578 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 610.5 bits (1573), Expect = 1.6e-174
Identity = 352/656 (53.66%), Postives = 443/656 (67.53%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           +++ WS  L          V  R N F C S+L      + P +K+  V  TR  + LGG
Sbjct: 48  RQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG 107

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLL 120
             P ++KL+PAV I+ FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLL
Sbjct: 108 -NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLL 167

Query: 121 WAGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETT 180
           W+G  L+CR LDP+VLP+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET 
Sbjct: 168 WSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETN 227

Query: 181 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 240
             +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI T
Sbjct: 228 NPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILT 287

Query: 241 NFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFT 300
           NFLSS+MIHATRPF++NEWIQTKI GYEVSGTVE VGWWSPTIIRG+DREAVHIPNHQF+
Sbjct: 288 NFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFS 347

Query: 301 INVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 360
           +N+VRNL+QKTHWRIKTHLAISHLDV+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE++
Sbjct: 348 VNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDI 407

Query: 361 NPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSD 420
           +PENQAL ILISCFVKTS FEEYLCVKEA++LDLL VIRHH ARLATPIRT+Q+M ++++
Sbjct: 408 DPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAE 467

Query: 421 LESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSG 480
           +++  FSD +F +    +NRR ++IEP YK+  +D  +S    S + G+++  P  +S  
Sbjct: 468 VDTAGFSDIVFNQAA--MNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS-- 527

Query: 481 DSKAAKEATPSDRKTEV-RTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSS------R 540
             +   +  PS+ K E    G    S+ KK  +     S+ N+  +    S+S      +
Sbjct: 528 -EERDLQEEPSETKAETENNGSVPVSNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQK 587

Query: 541 SASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVK 600
           S        G P    K   SD + + E   T +S+   GS K N +S            
Sbjct: 588 SEEKKKESVGDPHKAEKDEVSDDEATIE--QTLKSKAKQGSEKNNGESK----------A 647

Query: 601 KTGGSSATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSS 649
           + GG S TS                    +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 648 RDGGGSGTSSL------------------LEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of MS012578 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 610.5 bits (1573), Expect = 1.6e-174
Identity = 352/656 (53.66%), Postives = 443/656 (67.53%), Query Frame = 0

Query: 1   QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 60
           +++ WS  L          V  R N F C S+L      + P +K+  V  TR  + LGG
Sbjct: 48  RQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG 107

Query: 61  CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLL 120
             P ++KL+PAV I+ FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLL
Sbjct: 108 -NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLL 167

Query: 121 WAGVTLICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETT 180
           W+G  L+CR LDP+VLP+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET 
Sbjct: 168 WSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETN 227

Query: 181 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 240
             +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI T
Sbjct: 228 NPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILT 287

Query: 241 NFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFT 300
           NFLSS+MIHATRPF++NEWIQTKI GYEVSGTVE VGWWSPTIIRG+DREAVHIPNHQF+
Sbjct: 288 NFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGDDREAVHIPNHQFS 347

Query: 301 INVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 360
           +N+VRNL+QKTHWRIKTHLAISHLDV+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE++
Sbjct: 348 VNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDI 407

Query: 361 NPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSD 420
           +PENQAL ILISCFVKTS FEEYLCVKEA++LDLL VIRHH ARLATPIRT+Q+M ++++
Sbjct: 408 DPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAE 467

Query: 421 LESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSG 480
           +++  FSD +F +    +NRR ++IEP YK+  +D  +S    S + G+++  P  +S  
Sbjct: 468 VDTAGFSDIVFNQAA--MNRRYMLIEPSYKINSDDNSKS---PSPSPGQKSPSPGQKS-- 527

Query: 481 DSKAAKEATPSDRKTEV-RTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSS------R 540
             +   +  PS+ K E    G    S+ KK  +     S+ N+  +    S+S      +
Sbjct: 528 -EERDLQEEPSETKAETENNGSVPVSNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQK 587

Query: 541 SASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVK 600
           S        G P    K   SD + + E   T +S+   GS K N +S            
Sbjct: 588 SEEKKKESVGDPHKAEKDEVSDDEATIE--QTLKSKAKQGSEKNNGESK----------A 647

Query: 601 KTGGSSATSQPRIEGEQTPVSKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSS 649
           + GG S TS                    +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 648 RDGGGSGTSSL------------------LEENLVLGVALDGSKRTLPIDEEHKAS 664