MS012561 (gene) Bitter gourd (TR) v1

BLAST of MS012561 vs. NCBI nr
Match: XP_022136438.1 (protein NRT1/ PTR FAMILY 4.3-like [Momordica charantia])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 592/600 (98.67%), Postives = 595/600 (99.17%), Query Frame = 0

Query: 1   DEDHDDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS 60
           DED  DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS
Sbjct: 14  DEDR-DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS 73

Query: 61  LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLK 120
           LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGFVELSGFILLSVQAHLPQLK
Sbjct: 74  LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLK 133

Query: 121 PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS 180
           PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS
Sbjct: 134 PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS 193

Query: 181 KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR 240
           KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR
Sbjct: 194 KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR 253

Query: 241 NKPPQGPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK 300
           NKPPQGPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQN+VDHKVALSSDSANLIPTQK
Sbjct: 254 NKPPQGPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALSSDSANLIPTQK 313

Query: 301 --FLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSV 360
             FLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSV
Sbjct: 314 FRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSV 373

Query: 361 QQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRI 420
           QQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRI
Sbjct: 374 QQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRI 433

Query: 421 GFGLFLSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFY 480
           GFGLFLSTFSMVSAAIMEKKRRDSALNLNQT+SIFWITPQFLIFGLSEMFTAVGLIEFFY
Sbjct: 434 GFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFTAVGLIEFFY 493

Query: 481 KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY 540
           KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY
Sbjct: 494 KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY 553

Query: 541 WLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           WLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP
Sbjct: 554 WLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 611

BLAST of MS012561 vs. NCBI nr
Match: XP_023552004.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.2e-303
Identity = 536/595 (90.08%), Postives = 560/595 (94.12%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 23  EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 82

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           N+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH+PQLKPPQCN
Sbjct: 83  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 142

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           ML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ +P  SK LST
Sbjct: 143 MLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ-SPSQSKTLST 202

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAAVMA+GLISLV GIFYYRNKPPQ
Sbjct: 203 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 262

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           GPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQ S D KV LSS SANLIPTQK  FL+
Sbjct: 263 GPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQTSND-KVGLSSHSANLIPTQKFRFLN 322

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFACTI+FNTILAQLQTFSVQQGSL
Sbjct: 323 KACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSL 382

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF
Sbjct: 383 MNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 442

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LS FSMV+AAIMEKKRRDSA+NLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQS K
Sbjct: 443 LSAFSMVAAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSIK 502

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 503 GMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAA 562

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGEDYH YNFNSSK N+D+YVP
Sbjct: 563 LSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP 614

BLAST of MS012561 vs. NCBI nr
Match: XP_022929221.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.6e-303
Identity = 536/595 (90.08%), Postives = 559/595 (93.95%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 25  EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 84

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           N+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH+PQLKPPQCN
Sbjct: 85  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 144

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           ML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ +P  SK LST
Sbjct: 145 MLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ-SPTQSKTLST 204

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAAVMA+GLISLV GIFYYRNKPPQ
Sbjct: 205 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 264

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           GPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQ S D KV LSS SANLIPTQK  FL+
Sbjct: 265 GPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQTSND-KVGLSSHSANLIPTQKFRFLN 324

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFACTI+FNTILAQLQTFSVQQGSL
Sbjct: 325 KACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSL 384

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF
Sbjct: 385 MNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 444

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LS FSMVSAAIMEKKRRDSA+NLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFY QS K
Sbjct: 445 LSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIK 504

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 505 GMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAA 564

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGEDYH YNFNSSK N+D+YVP
Sbjct: 565 LSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP 616

BLAST of MS012561 vs. NCBI nr
Match: XP_022985329.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1047.0 bits (2706), Expect = 6.3e-302
Identity = 534/595 (89.75%), Postives = 557/595 (93.61%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 23  EDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 82

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           N+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH+PQLKPPQCN
Sbjct: 83  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 142

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           ML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ +P  SK LST
Sbjct: 143 MLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ-SPSQSKTLST 202

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAAVMA+GLISLV GIFYYRNKPPQ
Sbjct: 203 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 262

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           GPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQ S D KV LSS SANLIPTQK  FL+
Sbjct: 263 GPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQTSND-KVGLSSHSANLIPTQKFRFLN 322

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFACTI+FNTILAQLQTFSVQQGSL
Sbjct: 323 KACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSL 382

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKS HIPPASLQ+IPYIMLI IVPLYDTFFVPFARK TGHTSGISPLKRIGFGLF
Sbjct: 383 MNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFARKITGHTSGISPLKRIGFGLF 442

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LS FSMVSAAIMEKKRRDSA+NLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQS K
Sbjct: 443 LSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSIK 502

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 503 GMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAA 562

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           LSFLN LNYLFWS WFDEHPS+SQK  H++NPHHGEDYH YNFNSSK N+D+YVP
Sbjct: 563 LSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP 614

BLAST of MS012561 vs. NCBI nr
Match: KAG7015441.1 (Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.4e-301
Identity = 533/595 (89.58%), Postives = 556/595 (93.45%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 27  EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 86

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           N+VTNFVGTVFLLAL GGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH+PQLKPPQCN
Sbjct: 87  NIVTNFVGTVFLLALFGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 146

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           M   DG  C EAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ +P  SK LST
Sbjct: 147 MSESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ-SPTQSKTLST 206

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAAVMA+GLISLV GIFYYRNKPPQ
Sbjct: 207 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 266

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           GPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQ S D KV LSS SANLIPTQK  FL+
Sbjct: 267 GPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQTSND-KVGLSSHSANLIPTQKFRFLN 326

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFACTI+FNTILAQLQTFSVQQGSL
Sbjct: 327 KACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSL 386

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF
Sbjct: 387 MNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 446

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LS FSMVSAAIMEKKRRDSA+NL QTISIFWITPQFLIFGLSEMFTAVGLIEFFY QS K
Sbjct: 447 LSAFSMVSAAIMEKKRRDSAVNLTQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIK 506

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 507 GMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAA 566

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGEDYH YNFNSSK N+D+YVP
Sbjct: 567 LSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP 618

BLAST of MS012561 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 8.8e-238
Identity = 423/554 (76.35%), Postives = 480/554 (86.64%), Query Frame = 0

Query: 9   TVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVV 68
           +VDWRGRPSNP  HGGMRAA FVLGLQAFEIM IAAVGNNLITY INEMHF LSK+AN+V
Sbjct: 29  SVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIV 88

Query: 69  TNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLS 128
           TNFVGT+F+ AL+GG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+CN L 
Sbjct: 89  TNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLI 148

Query: 129 EDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFN 188
           +  + CEEAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQFS Q++PK SK+LS+YFN
Sbjct: 149 D--QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFS-QSHPKQSKRLSSYFN 208

Query: 189 AAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPI 248
           AAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV G  Y+RNK P+  I
Sbjct: 209 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSI 268

Query: 249 FTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVA---LSSDSANLIPTQKFLDKA 308
           FTPI  V+ VAA+LKRK   PS+P MLHG     DH VA   + S +    P  +FLDKA
Sbjct: 269 FTPIAHVI-VAAILKRKLASPSDPRMLHG-----DHHVANDVVPSSTLPHTPRFRFLDKA 328

Query: 309 CIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 368
           CIK QD  TKESPWRLCT TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS MN
Sbjct: 329 CIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 388

Query: 369 TELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 428
           T L+ SFHIPPASLQAIPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLS
Sbjct: 389 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 448

Query: 429 TFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSKGM 488
           TFSMVSAA++EKKRRDS++   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS+KGM
Sbjct: 449 TFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGM 508

Query: 489 QAFLTAITYCSYSFGFYLSSLLVSMVNKITATS-SNGGWLHDNNLNKDRLDLFYWLLAIL 548
           ++FL A+TYCSYSFGFY SS+LVS+VNKIT+TS  + GWL +N+LNKDRLDLFYWLLA+L
Sbjct: 509 ESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVL 568

Query: 549 SFLNFLNYLFWSTW 559
           S LNFL+YLFWS W
Sbjct: 569 SLLNFLSYLFWSRW 573

BLAST of MS012561 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 1.7e-220
Identity = 409/594 (68.86%), Postives = 478/594 (80.47%), Query Frame = 0

Query: 2   EDHDDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSL 61
           E+  D + VD+RGRP  P  HGG RAA FVLG QAFE+MAIAAVGNNLITY  NEMHF L
Sbjct: 19  EESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPL 78

Query: 62  SKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKP 121
           SKSAN+VTNF+GTVFLL+L+GGFLSDSYLGSF TML+FG +E+SGFILLSVQAHLP+L+P
Sbjct: 79  SKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP 138

Query: 122 PQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSK 181
           P+CNM S    +C EA G KA   + AL LVALGSGC+KPN+I+HGA+QF      K  +
Sbjct: 139 PECNMKSTT-IHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQF----QRKDLR 198

Query: 182 KLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRN 241
           KLS++FNAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+ISLV G  +YRN
Sbjct: 199 KLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRN 258

Query: 242 KPPQGPIFTPILQVVFVAALLKRKQVCPSNPHMLH-GSQNSVDHKVALSSDSANLIPTQK 301
           KPP G IFTPI Q VFVAA+ KRKQ+CPSNP+M+H  S + V  K  L S+        +
Sbjct: 259 KPPSGSIFTPIAQ-VFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKF------R 318

Query: 302 FLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQ 361
           FLDKACIK+Q G+  ESPWRLCT  QV QVKIL+SV+PIFACTIIFNTILAQLQTFSVQQ
Sbjct: 319 FLDKACIKTQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQ 378

Query: 362 GSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGF 421
           GS MNT +TK+F IPPASLQAIPYI+LIF VPLY+TFFVP ARK TG+ SGISPL+RIG 
Sbjct: 379 GSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGT 438

Query: 422 GLFLSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQ 481
           GLFL+TFSMV+AA++EKKRR+S L  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFYKQ
Sbjct: 439 GLFLATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQ 498

Query: 482 SSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNG---GWLHDNNLNKDRLDLF 541
           SS+ MQ+FLTA+TYCSYSFGFYLSS+LVS VN++T+++ +G   GWL DN+LNKDRLD F
Sbjct: 499 SSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHF 558

Query: 542 YWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKT 592
           YWLLA LSF+NF NYLFWS W+   PS +   HH    +  E   +     S T
Sbjct: 559 YWLLASLSFINFFNYLFWSRWYSCDPSAT---HHSAEVNSLEALENGEIKDSTT 596

BLAST of MS012561 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.3e-113
Identity = 238/568 (41.90%), Postives = 346/568 (60.92%), Query Frame = 0

Query: 11  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 70
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y    MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 71  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 130
           F+GT FLLAL+GGFLSD++  +F   LI   +E  G I+L++QA  P L PP C     D
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-----D 134

Query: 131 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 190
              CEE  G KA + FV LYLVALG G +K ++ +HGA+QF  ++ PK  K+ ST+FN  
Sbjct: 135 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFD-ESTPKGRKQRSTFFNYF 194

Query: 191 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 250
            F  + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G   T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 251 PILQVVFVAAL-----------LKRKQVCPSNPHMLHGSQNSVDHKVALSSD-SANLIPT 310
            IL+V+  A++           +    V PSN H +   +  V+ +  L        +P 
Sbjct: 255 TILKVLLAASVKCCSSGSSSNAVASMSVSPSN-HCVSKGKKEVESQGELEKPRQEEALPP 314

Query: 311 QKFLDKACIKSQDGETKESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQT 370
           +  L  + +K  +G   E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL T
Sbjct: 315 RAQLTNS-LKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 374

Query: 371 FSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPL 430
           FSVQQ + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L
Sbjct: 375 FSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 434

Query: 431 KRIGFGLFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--ISIFWITPQFLIFGLSEMF 490
           +RIG GL LS  +M  AA++E KR+  A     L+  +T  ++  WI  Q+L  G +++F
Sbjct: 435 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 494

Query: 491 TAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNN 550
           T  GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT +S N  WL   +
Sbjct: 495 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 554

Query: 551 LNKDRLDLFYWLLAILSFLNFLNYLFWS 557
           +N+ +LD FYWL+ +LS  NFL+YLFW+
Sbjct: 555 INRYKLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of MS012561 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.3e-108
Identity = 224/553 (40.51%), Postives = 337/553 (60.94%), Query Frame = 0

Query: 11  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 70
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y  N MH SL++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 71  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 130
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK--SSA 136

Query: 131 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 190
              CE   G KA   FV LYLV+LG G +K ++ +HGA+QF  +  PK  K+ ST+FN  
Sbjct: 137 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFD-EGTPKGRKQRSTFFNYY 196

Query: 191 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 250
            F  S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G   T
Sbjct: 197 VFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLT 256

Query: 251 PILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQKFLDKACIKSQ 310
            I +V+  A+++     C S     H +   V  +    + S +L  +   L+KA     
Sbjct: 257 TIFKVLLAASIVS----CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---- 316

Query: 311 DGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTELTK 370
           +G+T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  
Sbjct: 317 EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV- 376

Query: 371 SFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMV 430
           +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M 
Sbjct: 377 NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMA 436

Query: 431 SAAIMEKKRRDSA-----LNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 490
            AA++E KR+  A     L+  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++  
Sbjct: 437 VAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPS 496

Query: 491 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 550
            M++  T++++ S + G+YLSS++V +VN++T ++    WL +  LN++RLDLFYWL+ +
Sbjct: 497 SMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCV 555

Query: 551 LSFLNFLNYLFWS 557
           LS +NFL+YLFW+
Sbjct: 557 LSVVNFLHYLFWA 555

BLAST of MS012561 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.3e-102
Identity = 218/559 (39.00%), Postives = 333/559 (59.57%), Query Frame = 0

Query: 9   TVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVV 68
           TVD    P+N    G  +A  F+LG +  E +A   +G NL+ Y  + ++   + +AN V
Sbjct: 12  TVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 71

Query: 69  TNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLS 128
           TN+ GT ++  L+G F++D+YLG +WT+  F F+ +SG  LL++ A +P LKP  CN   
Sbjct: 72  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN--- 131

Query: 129 EDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFN 188
              + C       A +FFVALY++ALG+G +KP + + GADQF  +N+     K S++FN
Sbjct: 132 --ADTCHPNSSQTA-VFFVALYMIALGTGGIKPCVSSFGADQFD-ENDENEKIKKSSFFN 191

Query: 189 AAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPI 248
             YF+ +VG LIA T++VWIQ + G   GFGV    M + +     G  +YR + P G  
Sbjct: 192 WFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSP 251

Query: 249 FTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQ--KFLDKAC 308
            T I QV+ VAA  K     P +  +L  + +   +       S  L+ T   KF DKA 
Sbjct: 252 LTRIFQVI-VAAFRKISVKVPEDKSLLFETADDESN----IKGSRKLVHTDNLKFFDKAA 311

Query: 309 IKSQDGETKE---SPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 368
           ++SQ    K+   +PWRLC+ TQVE++K +I+++P++A  I+F T+ +Q+ T  V QG+ 
Sbjct: 312 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 371

Query: 369 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 428
           M+  + K+F IP ASL     + ++F  P+YD F +P ARKFT +  G + L+R+G GL 
Sbjct: 372 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 431

Query: 429 LSTFSMVSAAIMEKKRRDSALNLNQ------TISIFWITPQFLIFGLSEMFTAVGLIEFF 488
           +S F+M++A ++E  R D     N        +SIFW  PQ+L+ G +E+FT +G +EFF
Sbjct: 432 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 491

Query: 489 YKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLF 548
           Y Q+   M++  +A++  + + G YLS++LV++V KIT  +   GW+ D NLN+  LD F
Sbjct: 492 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYF 551

Query: 549 YWLLAILSFLNFLNYLFWS 557
           ++LLA LSFLNFL YL+ S
Sbjct: 552 FYLLATLSFLNFLVYLWIS 557

BLAST of MS012561 vs. ExPASy TrEMBL
Match: A0A6J1C7K1 (protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146 PE=3 SV=1)

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 592/600 (98.67%), Postives = 595/600 (99.17%), Query Frame = 0

Query: 1   DEDHDDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS 60
           DED  DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS
Sbjct: 14  DEDR-DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS 73

Query: 61  LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLK 120
           LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGFVELSGFILLSVQAHLPQLK
Sbjct: 74  LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLK 133

Query: 121 PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS 180
           PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS
Sbjct: 134 PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS 193

Query: 181 KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR 240
           KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR
Sbjct: 194 KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR 253

Query: 241 NKPPQGPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK 300
           NKPPQGPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQN+VDHKVALSSDSANLIPTQK
Sbjct: 254 NKPPQGPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALSSDSANLIPTQK 313

Query: 301 --FLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSV 360
             FLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSV
Sbjct: 314 FRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSV 373

Query: 361 QQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRI 420
           QQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRI
Sbjct: 374 QQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRI 433

Query: 421 GFGLFLSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFY 480
           GFGLFLSTFSMVSAAIMEKKRRDSALNLNQT+SIFWITPQFLIFGLSEMFTAVGLIEFFY
Sbjct: 434 GFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFTAVGLIEFFY 493

Query: 481 KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY 540
           KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY
Sbjct: 494 KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY 553

Query: 541 WLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           WLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP
Sbjct: 554 WLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 611

BLAST of MS012561 vs. ExPASy TrEMBL
Match: A0A6J1EMI3 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 536/595 (90.08%), Postives = 559/595 (93.95%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 25  EDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 84

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           N+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH+PQLKPPQCN
Sbjct: 85  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 144

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           ML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ +P  SK LST
Sbjct: 145 MLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ-SPTQSKTLST 204

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAAVMA+GLISLV GIFYYRNKPPQ
Sbjct: 205 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 264

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           GPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQ S D KV LSS SANLIPTQK  FL+
Sbjct: 265 GPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQTSND-KVGLSSHSANLIPTQKFRFLN 324

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFACTI+FNTILAQLQTFSVQQGSL
Sbjct: 325 KACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSL 384

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF
Sbjct: 385 MNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 444

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LS FSMVSAAIMEKKRRDSA+NLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFY QS K
Sbjct: 445 LSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYNQSIK 504

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 505 GMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAA 564

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGEDYH YNFNSSK N+D+YVP
Sbjct: 565 LSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP 616

BLAST of MS012561 vs. ExPASy TrEMBL
Match: A0A6J1JCZ7 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 3.1e-302
Identity = 534/595 (89.75%), Postives = 557/595 (93.61%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 23  EDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYLINEMHFSLSKSA 82

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           N+VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAH+PQLKPPQCN
Sbjct: 83  NIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHVPQLKPPQCN 142

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           ML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ +P  SK LST
Sbjct: 143 MLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQ-SPSQSKTLST 202

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAAVMA+GLISLV GIFYYRNKPPQ
Sbjct: 203 YFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGLISLVAGIFYYRNKPPQ 262

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           GPIFTPILQ VFVAALLKRKQVCPSNPHMLHGSQ S D KV LSS SANLIPTQK  FL+
Sbjct: 263 GPIFTPILQ-VFVAALLKRKQVCPSNPHMLHGSQTSND-KVGLSSHSANLIPTQKFRFLN 322

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFACTI+FNTILAQLQTFSVQQGSL
Sbjct: 323 KACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTIVFNTILAQLQTFSVQQGSL 382

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKS HIPPASLQ+IPYIMLI IVPLYDTFFVPFARK TGHTSGISPLKRIGFGLF
Sbjct: 383 MNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFARKITGHTSGISPLKRIGFGLF 442

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LS FSMVSAAIMEKKRRDSA+NLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQS K
Sbjct: 443 LSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSIK 502

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 503 GMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNLNKDRLDLFYWLLAA 562

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP 599
           LSFLN LNYLFWS WFDEHPS+SQK  H++NPHHGEDYH YNFNSSK N+D+YVP
Sbjct: 563 LSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHIYNFNSSKNNVDDYVP 614

BLAST of MS012561 vs. ExPASy TrEMBL
Match: A0A6J1FR74 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 1.1e-291
Identity = 525/577 (90.99%), Postives = 549/577 (95.15%), Query Frame = 0

Query: 8   VTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANV 67
           +TVDWRGRPSNP++HGGMRAAAFVLGLQ+FEIMAIAAVGNNLITY INEMHFSLSKSANV
Sbjct: 25  LTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANV 84

Query: 68  VTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNML 127
           VTNFVGTVFLLAL+GGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNML
Sbjct: 85  VTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNML 144

Query: 128 SEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYF 187
           SE G++C EAKG+KALIFFVALYLVALGSGCVKPNMIAHGADQF+PQ +P  SK+LSTYF
Sbjct: 145 SE-GDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQ-DPSQSKRLSTYF 204

Query: 188 NAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGP 247
           NAAYFAFS+GELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 
Sbjct: 205 NAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGA 264

Query: 248 IFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLDKA 307
           IF PILQ VFVAA+LKRKQVCPSNP MLHGSQ S +HKVALSS SANLIPTQK  FLDKA
Sbjct: 265 IFIPILQ-VFVAAVLKRKQVCPSNPQMLHGSQTS-NHKVALSSHSANLIPTQKFRFLDKA 324

Query: 308 CIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 367
           CIKSQDG T+ESPWRLCT TQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN
Sbjct: 325 CIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 384

Query: 368 TELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 427
           T+LTKSFHIPPASLQ+IPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS
Sbjct: 385 TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 444

Query: 428 TFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSKGM 487
           TFSMVSAAIMEKKRRDSA+N NQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQS KGM
Sbjct: 445 TFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 504

Query: 488 QAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAILS 547
           QAFLTAITYCSYSFGFYLSSLLVSMVNKIT++S+NGGWLHDNNLNKDRLDLFYWLLA LS
Sbjct: 505 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAALS 564

Query: 548 FLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 583
           FLNFLNYLFWS+WF + PSLSQK   D N HHGE+ H
Sbjct: 565 FLNFLNYLFWSSWFQDPPSLSQKLQQD-NLHHGENSH 596

BLAST of MS012561 vs. ExPASy TrEMBL
Match: A0A6J1JRW4 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.2e-291
Identity = 523/579 (90.33%), Postives = 549/579 (94.82%), Query Frame = 0

Query: 6   DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSA 65
           + +TVDWRGRPSNP++HGGMRAAAFVLGLQ+FEIMAIAAVGNNLITY INEMHFSLSKSA
Sbjct: 21  EDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSA 80

Query: 66  NVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 125
           NVVTNFVGTVFLLAL+GGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN
Sbjct: 81  NVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCN 140

Query: 126 MLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLST 185
           MLSE G++C EAKG+KALIFFVALYLVALGSGCVKPNMIAHGADQF+PQ +P  SK+LST
Sbjct: 141 MLSE-GDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQ-DPSQSKRLST 200

Query: 186 YFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 245
           YFNAAYFAFS+GELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ
Sbjct: 201 YFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQ 260

Query: 246 GPIFTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQK--FLD 305
           G +F PILQ VFVAA+LKRKQVCPSNP MLHGSQ S +HKVALSS SANLIPTQK  FLD
Sbjct: 261 GALFIPILQ-VFVAAVLKRKQVCPSNPQMLHGSQTS-NHKVALSSHSANLIPTQKFRFLD 320

Query: 306 KACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 365
           KACIKS DG T+ESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL
Sbjct: 321 KACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 380

Query: 366 MNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 425
           MNT+LTKSFHIPPASLQ+IPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF
Sbjct: 381 MNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 440

Query: 426 LSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 485
           LSTFSMVSAAIMEKKRRDSA+N NQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQS K
Sbjct: 441 LSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVK 500

Query: 486 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 545
           GMQAFLTAITYCSYSFGFYLSSLLVSMVNKIT++S+NGGWLHDNNLNKDRLDLFYWLLA 
Sbjct: 501 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAA 560

Query: 546 LSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 583
           LSFLNFLNYLFWS+WF + P LSQK   D N HHGE+ H
Sbjct: 561 LSFLNFLNYLFWSSWFQDPPPLSQKLQQD-NLHHGENPH 594

BLAST of MS012561 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 824.3 bits (2128), Expect = 6.2e-239
Identity = 423/554 (76.35%), Postives = 480/554 (86.64%), Query Frame = 0

Query: 9   TVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVV 68
           +VDWRGRPSNP  HGGMRAA FVLGLQAFEIM IAAVGNNLITY INEMHF LSK+AN+V
Sbjct: 29  SVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIV 88

Query: 69  TNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLS 128
           TNFVGT+F+ AL+GG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+CN L 
Sbjct: 89  TNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLI 148

Query: 129 EDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFN 188
           +  + CEEAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQFS Q++PK SK+LS+YFN
Sbjct: 149 D--QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFS-QSHPKQSKRLSSYFN 208

Query: 189 AAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPI 248
           AAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV G  Y+RNK P+  I
Sbjct: 209 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSI 268

Query: 249 FTPILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVA---LSSDSANLIPTQKFLDKA 308
           FTPI  V+ VAA+LKRK   PS+P MLHG     DH VA   + S +    P  +FLDKA
Sbjct: 269 FTPIAHVI-VAAILKRKLASPSDPRMLHG-----DHHVANDVVPSSTLPHTPRFRFLDKA 328

Query: 309 CIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 368
           CIK QD  TKESPWRLCT TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS MN
Sbjct: 329 CIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 388

Query: 369 TELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 428
           T L+ SFHIPPASLQAIPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLS
Sbjct: 389 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 448

Query: 429 TFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSKGM 488
           TFSMVSAA++EKKRRDS++   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS+KGM
Sbjct: 449 TFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGM 508

Query: 489 QAFLTAITYCSYSFGFYLSSLLVSMVNKITATS-SNGGWLHDNNLNKDRLDLFYWLLAIL 548
           ++FL A+TYCSYSFGFY SS+LVS+VNKIT+TS  + GWL +N+LNKDRLDLFYWLLA+L
Sbjct: 509 ESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVL 568

Query: 549 SFLNFLNYLFWSTW 559
           S LNFL+YLFWS W
Sbjct: 569 SLLNFLSYLFWSRW 573

BLAST of MS012561 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 766.9 bits (1979), Expect = 1.2e-221
Identity = 409/594 (68.86%), Postives = 478/594 (80.47%), Query Frame = 0

Query: 2   EDHDDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSL 61
           E+  D + VD+RGRP  P  HGG RAA FVLG QAFE+MAIAAVGNNLITY  NEMHF L
Sbjct: 19  EESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPL 78

Query: 62  SKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKP 121
           SKSAN+VTNF+GTVFLL+L+GGFLSDSYLGSF TML+FG +E+SGFILLSVQAHLP+L+P
Sbjct: 79  SKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP 138

Query: 122 PQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSK 181
           P+CNM S    +C EA G KA   + AL LVALGSGC+KPN+I+HGA+QF      K  +
Sbjct: 139 PECNMKSTT-IHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQF----QRKDLR 198

Query: 182 KLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRN 241
           KLS++FNAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+ISLV G  +YRN
Sbjct: 199 KLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRN 258

Query: 242 KPPQGPIFTPILQVVFVAALLKRKQVCPSNPHMLH-GSQNSVDHKVALSSDSANLIPTQK 301
           KPP G IFTPI Q VFVAA+ KRKQ+CPSNP+M+H  S + V  K  L S+        +
Sbjct: 259 KPPSGSIFTPIAQ-VFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKF------R 318

Query: 302 FLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQ 361
           FLDKACIK+Q G+  ESPWRLCT  QV QVKIL+SV+PIFACTIIFNTILAQLQTFSVQQ
Sbjct: 319 FLDKACIKTQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQ 378

Query: 362 GSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGF 421
           GS MNT +TK+F IPPASLQAIPYI+LIF VPLY+TFFVP ARK TG+ SGISPL+RIG 
Sbjct: 379 GSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGT 438

Query: 422 GLFLSTFSMVSAAIMEKKRRDSALNLNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQ 481
           GLFL+TFSMV+AA++EKKRR+S L  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFYKQ
Sbjct: 439 GLFLATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQ 498

Query: 482 SSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNG---GWLHDNNLNKDRLDLF 541
           SS+ MQ+FLTA+TYCSYSFGFYLSS+LVS VN++T+++ +G   GWL DN+LNKDRLD F
Sbjct: 499 SSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHF 558

Query: 542 YWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKT 592
           YWLLA LSF+NF NYLFWS W+   PS +   HH    +  E   +     S T
Sbjct: 559 YWLLASLSFINFFNYLFWSRWYSCDPSAT---HHSAEVNSLEALENGEIKDSTT 596

BLAST of MS012561 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 411.0 bits (1055), Expect = 1.6e-114
Identity = 238/568 (41.90%), Postives = 346/568 (60.92%), Query Frame = 0

Query: 11  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 70
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y    MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 71  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 130
           F+GT FLLAL+GGFLSD++  +F   LI   +E  G I+L++QA  P L PP C     D
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-----D 134

Query: 131 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 190
              CEE  G KA + FV LYLVALG G +K ++ +HGA+QF  ++ PK  K+ ST+FN  
Sbjct: 135 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFD-ESTPKGRKQRSTFFNYF 194

Query: 191 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 250
            F  + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G   T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 251 PILQVVFVAAL-----------LKRKQVCPSNPHMLHGSQNSVDHKVALSSD-SANLIPT 310
            IL+V+  A++           +    V PSN H +   +  V+ +  L        +P 
Sbjct: 255 TILKVLLAASVKCCSSGSSSNAVASMSVSPSN-HCVSKGKKEVESQGELEKPRQEEALPP 314

Query: 311 QKFLDKACIKSQDGETKESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQT 370
           +  L  + +K  +G   E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL T
Sbjct: 315 RAQLTNS-LKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 374

Query: 371 FSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPL 430
           FSVQQ + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L
Sbjct: 375 FSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 434

Query: 431 KRIGFGLFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--ISIFWITPQFLIFGLSEMF 490
           +RIG GL LS  +M  AA++E KR+  A     L+  +T  ++  WI  Q+L  G +++F
Sbjct: 435 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 494

Query: 491 TAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNN 550
           T  GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT +S N  WL   +
Sbjct: 495 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 554

Query: 551 LNKDRLDLFYWLLAILSFLNFLNYLFWS 557
           +N+ +LD FYWL+ +LS  NFL+YLFW+
Sbjct: 555 INRYKLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of MS012561 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 9.3e-110
Identity = 224/553 (40.51%), Postives = 337/553 (60.94%), Query Frame = 0

Query: 11  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 70
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y  N MH SL++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 71  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 130
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK--SSA 132

Query: 131 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 190
              CE   G KA   FV LYLV+LG G +K ++ +HGA+QF  +  PK  K+ ST+FN  
Sbjct: 133 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFD-EGTPKGRKQRSTFFNYY 192

Query: 191 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 250
            F  S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G   T
Sbjct: 193 VFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLT 252

Query: 251 PILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQKFLDKACIKSQ 310
            I +V+  A+++     C S     H +   V  +    + S +L  +   L+KA     
Sbjct: 253 TIFKVLLAASIVS----CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---- 312

Query: 311 DGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTELTK 370
           +G+T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  
Sbjct: 313 EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV- 372

Query: 371 SFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMV 430
           +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M 
Sbjct: 373 NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMA 432

Query: 431 SAAIMEKKRRDSA-----LNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 490
            AA++E KR+  A     L+  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++  
Sbjct: 433 VAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPS 492

Query: 491 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 550
            M++  T++++ S + G+YLSS++V +VN++T ++    WL +  LN++RLDLFYWL+ +
Sbjct: 493 SMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCV 551

Query: 551 LSFLNFLNYLFWS 557
           LS +NFL+YLFW+
Sbjct: 553 LSVVNFLHYLFWA 551

BLAST of MS012561 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 9.3e-110
Identity = 224/553 (40.51%), Postives = 337/553 (60.94%), Query Frame = 0

Query: 11  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 70
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y  N MH SL++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 71  FVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 130
           F+ T FLLAL+GGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK--SSA 136

Query: 131 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 190
              CE   G KA   FV LYLV+LG G +K ++ +HGA+QF  +  PK  K+ ST+FN  
Sbjct: 137 ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFD-EGTPKGRKQRSTFFNYY 196

Query: 191 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 250
            F  S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G   T
Sbjct: 197 VFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLT 256

Query: 251 PILQVVFVAALLKRKQVCPSNPHMLHGSQNSVDHKVALSSDSANLIPTQKFLDKACIKSQ 310
            I +V+  A+++     C S     H +   V  +    + S +L  +   L+KA     
Sbjct: 257 TIFKVLLAASIVS----CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---- 316

Query: 311 DGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTELTK 370
           +G+T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  
Sbjct: 317 EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV- 376

Query: 371 SFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMV 430
           +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M 
Sbjct: 377 NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMA 436

Query: 431 SAAIMEKKRRDSA-----LNLNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSK 490
            AA++E KR+  A     L+  +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++  
Sbjct: 437 VAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPS 496

Query: 491 GMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAI 550
            M++  T++++ S + G+YLSS++V +VN++T ++    WL +  LN++RLDLFYWL+ +
Sbjct: 497 SMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCV 555

Query: 551 LSFLNFLNYLFWS 557
           LS +NFL+YLFW+
Sbjct: 557 LSVVNFLHYLFWA 555