MS012533 (gene) Bitter gourd (TR) v1

BLAST of MS012533 vs. NCBI nr
Match: XP_022136370.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 781.2 bits (2016), Expect = 4.2e-222
Identity = 383/386 (99.22%), Postives = 386/386 (100.00%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLK+LIVSNPSKSLP
Sbjct: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIVSNPSKSLP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN
Sbjct: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR
Sbjct: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLP+GFDFEKKVM
Sbjct: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPQGFDFEKKVM 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV
Sbjct: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKEA 387
           RRPRPGIILASSRGKRQNNPYSNKEA
Sbjct: 361 RRPRPGIILASSRGKRQNNPYSNKEA 386

BLAST of MS012533 vs. NCBI nr
Match: XP_022994938.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita maxima])

HSP 1 Score: 686.0 bits (1769), Expect = 1.8e-193
Identity = 331/385 (85.97%), Postives = 357/385 (92.73%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MILR TR P+RL QTR FVDAKVKWVRDPYLDF VQREKNLRQVISLK+LIVS+P KS+P
Sbjct: 1   MILRVTRAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSL++QNLK PTT++SKFFQLYPSVF QFQPS GLHP VKLT Q+L LHKEE TIHN
Sbjct: 61  LSSVSLMRQNLKAPTTSISKFFQLYPSVFIQFQPSLGLHPHVKLTSQSLALHKEELTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDD+VKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPYKFIP LLADYPEYFQVC++R
Sbjct: 121 SQPHRDDIVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPALLADYPEYFQVCSMR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALE+LSWR++LS+SELK+R   +GN W R GNHIAFPMS PRGFD EKKV+
Sbjct: 181 DCSTGEQTLALELLSWREDLSVSELKKRHSLEGNGWRRNGNHIAFPMSFPRGFDLEKKVL 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWT AVLHELLYL+VSKKTEKENIFCLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMNK V
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNKAV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           RRPRP IILASSRGKRQNNP +NKE
Sbjct: 361 RRPRPRIILASSRGKRQNNPSANKE 385

BLAST of MS012533 vs. NCBI nr
Match: KAG7031364.1 (Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 683.3 bits (1762), Expect = 1.2e-192
Identity = 333/385 (86.49%), Postives = 356/385 (92.47%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MIL  TR P+RL QTR FVDAKVKWVRDPYLDF VQREKNLRQVISLK+LIVS+P KS+P
Sbjct: 1   MILGVTRAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSL++QNLKVPTT++SKF QLYPSVF QFQPS GLHP VKLT QAL LHKEESTIHN
Sbjct: 61  LSSVSLMRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKLTSQALALHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDDVVKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPYKFIPTLLADYPEYFQVC+VR
Sbjct: 121 SQPHRDDVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPTLLADYPEYFQVCSVR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALE+LSWR++LS+SELK+R   +GN W R G HIAFPMS PRGFD EKKV+
Sbjct: 181 DCSTGEQTLALELLSWREDLSVSELKKRHSLEGNGWRRNGIHIAFPMSFPRGFDLEKKVL 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWT AVLHELLYL+VSKKTEKENIFCLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMNK V
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNKAV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           RRPRP IILASSR KRQNNP +NKE
Sbjct: 361 RRPRPRIILASSRDKRQNNPSANKE 385

BLAST of MS012533 vs. NCBI nr
Match: XP_023536124.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 678.7 bits (1750), Expect = 2.9e-191
Identity = 330/385 (85.71%), Postives = 355/385 (92.21%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MIL  TR P+RL QTR FVDAKVKWVRDPYLDF VQREKNLRQVISLK+LIVS+P KS+P
Sbjct: 1   MILGVTRAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSL++QNLKVPTT++SKF QLYPSVF QFQPS GLHP VK+T QAL LHKEES IHN
Sbjct: 61  LSSVSLMRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKITSQALALHKEESAIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDDVVKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPYKFIP LLADYPEYFQVC+VR
Sbjct: 121 SQPHRDDVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPALLADYPEYFQVCSVR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           D STGEQTLALE+LSWR++LS+SELK+R   +GN W R GNHIAFPMS PRGFD EKKV+
Sbjct: 181 DRSTGEQTLALELLSWREDLSVSELKKRHSLEGNGWRRNGNHIAFPMSFPRGFDLEKKVL 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWT AVLHELLYL+VSKKTEKENIFCLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMN+ V
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNQAV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           RRPRP IILASSRGKRQNNP +NKE
Sbjct: 361 RRPRPRIILASSRGKRQNNPSANKE 385

BLAST of MS012533 vs. NCBI nr
Match: XP_022941952.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita moschata])

HSP 1 Score: 675.6 bits (1742), Expect = 2.5e-190
Identity = 330/385 (85.71%), Postives = 354/385 (91.95%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MIL  TR P+RL QTR FVDAKVKWVRDPYLDF VQRE+NLRQVISLK+LIVS+P KS+P
Sbjct: 1   MILGVTRAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREENLRQVISLKNLIVSSPFKSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSL++QNLKVPTT++SKF QLYPSVF QFQPS GLHP VKLT QAL LHKEESTIHN
Sbjct: 61  LSSVSLMRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKLTSQALALHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDDVVKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPYKFIPTLLADYPEYFQVC+VR
Sbjct: 121 SQPHRDDVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPTLLADYPEYFQVCSVR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALE+LSWR++LS+SELK+R   +GN W R G HIAFPMS PRGFD EKKV+
Sbjct: 181 DCSTGEQTLALELLSWREDLSVSELKKRHSLEGNGWRRNGIHIAFPMSFPRGFDLEKKVL 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWT AVLHELLYL+VSKKTEKENI CLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENISCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYI LMNK V
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIRLMNKAV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           RRPRP IILASSR KRQNNP +NKE
Sbjct: 361 RRPRPRIILASSRDKRQNNPSANKE 385

BLAST of MS012533 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.9e-53
Identity = 122/347 (35.16%), Postives = 194/347 (55.91%), Query Frame = 0

Query: 12  LSQTRGFVDAKVKWVRDPYLDFA--VQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQ 71
           L+Q R +VD  +KW RDPY D    + R   L+ V+SLK+ IV  P++ +P+S++S   +
Sbjct: 18  LTQKRTYVDVYMKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTR 77

Query: 72  NLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHNSQPHRDDVV 131
              V +T ++ F + +PS+F +F       P  +LTP+A  L ++E  ++  Q   DD+ 
Sbjct: 78  QFDV-STKIAHFLRKFPSIFEEFVGPEYNLPWFRLTPEATELDRQERVVY--QTSADDLR 137

Query: 132 KRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCSTGEQTL 191
            RL KL++++    LPL +++ ++W LGLP  ++     +    F+   + D   G + L
Sbjct: 138 DRLKKLILMSKDNVLPLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMED---GVKGL 197

Query: 192 ALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVMNWVEEWQRL 251
           A++     K LS+ +    +   G V       I FP+   +G     K+ +W+ E+Q+L
Sbjct: 198 AVDYNGGDKVLSVLQKNAMKKRRGEV---SLEEIEFPLFPSKGCRLRVKIEDWLMEFQKL 257

Query: 252 PYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKKA 311
           PY+SPY++   L P+SD AEK  V  LHELL L V    E++ + CL ++ G   +  KA
Sbjct: 258 PYVSPYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKA 317

Query: 312 LVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLM 357
              HP IFY+S K +T T +LRE YR    VE HP++G+R +YI LM
Sbjct: 318 FERHPQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKKYIQLM 355

BLAST of MS012533 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.6e-35
Identity = 113/358 (31.56%), Postives = 183/358 (51.12%), Query Frame = 0

Query: 19  VDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQNLKVPTTTV 78
           V A VK  ++   D  VQR+K L+ V++++ ++VS P + + L  +   +++L +     
Sbjct: 72  VRAAVKRRKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRDLGLKKR-- 131

Query: 79  SKFFQL---YPSVFTQFQPSPGLHPL-VKLTPQALTLHKEESTIHNSQPHRDDVVKRLAK 138
            +F  L   YP VF   +   G + L  K+T +A  L+ +E  I N     D +V +L K
Sbjct: 132 RRFIALLRKYPGVFEIVE--EGAYSLRFKMTSEAERLYLDEMRIRNEL--EDVLVVKLRK 191

Query: 139 LLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCSTGEQTLALEIL 198
           L+M++   R+ L  I  L+ DLGLP +F  T+   YP+YF+V          +  ALE+ 
Sbjct: 192 LVMMSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPT------PRGPALELT 251

Query: 199 SWRKELSISELKRRECPDGNVWSRKGNHIA------FPMSLPRGFDFEKKVMNWVEEWQR 258
            W  EL++S  +  E  +    S + N I         + LPRG +  K     + +++ 
Sbjct: 252 HWDPELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETRKISQFRD 311

Query: 259 LPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKK 318
           + YISPY++  HL   + + EK    V+HELL L   K+T  +++    E   F  + + 
Sbjct: 312 MQYISPYKDFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEFRFSQQLRG 371

Query: 319 ALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVVRRPRPG 367
            L+ HP +FYVS K    +V LREAYR   L++K PL  ++ +   L++ V R PR G
Sbjct: 372 MLIRHPDLFYVSLKGERDSVFLREAYRNSELIDKDPLTLVKEKMRALVS-VPRFPRRG 416

BLAST of MS012533 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 5.3e-34
Identity = 112/370 (30.27%), Postives = 186/370 (50.27%), Query Frame = 0

Query: 21  AKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQNLKVP-TTTVS 80
           A VK  ++   D  +QR+K L+ V+ L++++VSNP + + L  +   +++L +     + 
Sbjct: 59  AAVKRRKEIPFDNVIQRDKKLKLVLKLRNILVSNPDRVMSLRDLGRFRRDLGLTRKRRLI 118

Query: 81  KFFQLYPSVFTQFQPSPGLHPL-VKLTPQALTLHKEESTIHNSQPHRDDVVKRLAKLLML 140
              + +P VF   +   G++ L  +LTP A  L+ +E  +H         V +L KLLM+
Sbjct: 119 ALLKRFPGVFEVVE--EGVYSLRFRLTPAAERLYLDE--LHLKNESEGLAVTKLRKLLMM 178

Query: 141 TGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCSTGEQTLALEILSWRK 200
           +   R+ +  I  L+ DLGLP +F  T+   YP+YF+V  +      ++   LE+  W  
Sbjct: 179 SQDKRILIEKIAHLKNDLGLPPEFRDTICLRYPQYFRVVQM------DRGPGLELTHWDP 238

Query: 201 ELSIS-------ELKRRECPDGNV---WSRKGNHIAFPMSLPRGFDFEKKVMNWVEEWQR 260
           EL++S       E + RE  + N+      K N +     LP+G    +     V +++ 
Sbjct: 239 ELAVSAAEVAEEENRAREEQERNLIIDRPLKFNRV----KLPQGLKLSRGEARRVAQFKE 298

Query: 261 LPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKK 320
           +PYISPY +  HL   S + EK    V+HE+L L + K+T  +++    E   F    + 
Sbjct: 299 MPYISPYSDFSHLRSGSAEKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRG 358

Query: 321 ALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVVRRPRPGII 379
            L+ HP +FYVS K    +V LREAY+   LVEK  L+ ++ + +  +  V R PR G+ 
Sbjct: 359 MLIRHPDMFYVSLKGDRDSVFLREAYKNSQLVEKSKLVLLKEK-MRALVAVPRFPRRGVP 413

BLAST of MS012533 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.2e-33
Identity = 107/377 (28.38%), Postives = 188/377 (49.87%), Query Frame = 0

Query: 21  AKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQNLKVP-TTTVS 80
           A VK  ++   D  +QR+K L+ V+ L++++V+ P + + L  +   +++L +     + 
Sbjct: 53  AAVKRRKEAPFDTVIQRDKKLKLVLKLRNILVAQPDRVMSLRELGRFRRDLGLTRKRRLI 112

Query: 81  KFFQLYPSVFTQFQPSPGLHPL-VKLTPQALTLHKEESTIHNSQPHRDDVVKRLAKLLML 140
              + +P VF   +   G++ L  +LTP A  L+ +E  + N        V +L KLLM+
Sbjct: 113 ALLRRFPGVFDVVE--EGVYSLRFRLTPAAERLYLDELRLRNES--EGLAVAKLRKLLMM 172

Query: 141 TGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCSTGEQTLALEILSWRK 200
           +   R+ +  +  L+ DLGLP +F  T+   YP+YF+V  +      ++  ALE+  W  
Sbjct: 173 SQEKRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRM------DRGPALELTHWDP 232

Query: 201 ELSIS-------ELKRRECPDGNV---WSRKGNHIAFPMSLPRGFDFEKKVMNWVEEWQR 260
           EL++S       E + RE  + N+      K N +     LP+G    +     +  ++ 
Sbjct: 233 ELAVSAAELAEEESRAREAEERNLIIDRPLKFNRV----RLPKGLKLTRGEARRIARFKE 292

Query: 261 LPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKK 320
           +PYISPY +  HL   SD+ EK    V+HE+L L V K+T  +++    E   F    + 
Sbjct: 293 MPYISPYADFSHLRSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRG 352

Query: 321 ALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVVRRPRPGII 380
            ++ HP +FYVS K    +V LREAY+   LVEK+ L+ ++ + +  +  V R PR   +
Sbjct: 353 MIIRHPDMFYVSFKGDRDSVFLREAYKDSQLVEKNQLVLLKEK-MRALVAVPRFPRRAAV 412

Query: 381 LASSRGKRQNNPYSNKE 386
                 +  N    +++
Sbjct: 413 GTGEEAEGMNGSLQSRD 414

BLAST of MS012533 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 7.4e-28
Identity = 112/368 (30.43%), Postives = 175/368 (47.55%), Query Frame = 0

Query: 9   PYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLK 68
           P+  S T   +  K   VRD   D  ++ EK +R+V+   SLI+S P+ ++ +S +  L 
Sbjct: 33  PFSQSTT---IPKKQDRVRDHGYDNYMEVEKKIRKVVKFHSLILSQPNHTIAISLLDTLA 92

Query: 69  QNLKV--PTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHNSQPHRD 128
           + L +         F   +P VF  ++     HP+ ++    LT    +   H  +   D
Sbjct: 93  RRLGLGFKQHEPGAFLLKFPHVFEIYE-----HPVQRILYCRLTRKALDQIRHEHEAVLD 152

Query: 129 ---DVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCS 188
              D V RL KL+M++  GR+ L  +   + + GLP  F  +++  +P++F++       
Sbjct: 153 QIPDAVTRLRKLVMMSNTGRIRLEHVRIARTEFGLPEDFEYSVILKHPQFFRLI------ 212

Query: 189 TGEQT--LALEILSWRKELSISELKR-RECPDGNVWSRKG-----NHIAFPMSLPRGFDF 248
            GE+T    +EI+     LSI  ++R RE      +  KG        +F ++ P GF  
Sbjct: 213 DGEETRDKYIEIVEKDPNLSICAIERVREIE----YRTKGIDAEDVRFSFVVNFPPGFKI 272

Query: 249 EKKVMNWVEEWQRLPYISPYENAFHLAPNSDQA----EKWTVAVLHELLYLLVSKKTEKE 308
            K     V +WQRLPY SPYE+       S +A    EK +VA +HELL L V KK   E
Sbjct: 273 GKYFRIAVWKWQRLPYWSPYEDISGYDLRSMEAQNRLEKRSVACIHELLSLTVEKKITLE 332

Query: 309 NIFCLGEYMGFGSRFKKALVHHPGIFYVS---NKIRTQTVVLREAYRKDFLVEKHPLMGM 357
            I      M    + K+ L+ H GIFY+S   N  +  TV LRE Y++  LVE + +   
Sbjct: 333 RIAHFRNVMNLPKKLKEFLLQHQGIFYISTRGNYGKLHTVFLREGYKRGELVEPNDVYLA 382

BLAST of MS012533 vs. ExPASy TrEMBL
Match: A0A6J1C3P8 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111008095 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.0e-222
Identity = 383/386 (99.22%), Postives = 386/386 (100.00%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLK+LIVSNPSKSLP
Sbjct: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIVSNPSKSLP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN
Sbjct: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR
Sbjct: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLP+GFDFEKKVM
Sbjct: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPQGFDFEKKVM 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV
Sbjct: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKEA 387
           RRPRPGIILASSRGKRQNNPYSNKEA
Sbjct: 361 RRPRPGIILASSRGKRQNNPYSNKEA 386

BLAST of MS012533 vs. ExPASy TrEMBL
Match: A0A6J1K6G9 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111490493 PE=4 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 8.9e-194
Identity = 331/385 (85.97%), Postives = 357/385 (92.73%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MILR TR P+RL QTR FVDAKVKWVRDPYLDF VQREKNLRQVISLK+LIVS+P KS+P
Sbjct: 1   MILRVTRAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSL++QNLK PTT++SKFFQLYPSVF QFQPS GLHP VKLT Q+L LHKEE TIHN
Sbjct: 61  LSSVSLMRQNLKAPTTSISKFFQLYPSVFIQFQPSLGLHPHVKLTSQSLALHKEELTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDD+VKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPYKFIP LLADYPEYFQVC++R
Sbjct: 121 SQPHRDDIVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPALLADYPEYFQVCSMR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALE+LSWR++LS+SELK+R   +GN W R GNHIAFPMS PRGFD EKKV+
Sbjct: 181 DCSTGEQTLALELLSWREDLSVSELKKRHSLEGNGWRRNGNHIAFPMSFPRGFDLEKKVL 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWT AVLHELLYL+VSKKTEKENIFCLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMNK V
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNKAV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           RRPRP IILASSRGKRQNNP +NKE
Sbjct: 361 RRPRPRIILASSRGKRQNNPSANKE 385

BLAST of MS012533 vs. ExPASy TrEMBL
Match: A0A6J1FNW3 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111447163 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 330/385 (85.71%), Postives = 354/385 (91.95%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MIL  TR P+RL QTR FVDAKVKWVRDPYLDF VQRE+NLRQVISLK+LIVS+P KS+P
Sbjct: 1   MILGVTRAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREENLRQVISLKNLIVSSPFKSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSL++QNLKVPTT++SKF QLYPSVF QFQPS GLHP VKLT QAL LHKEESTIHN
Sbjct: 61  LSSVSLMRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKLTSQALALHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           SQPHRDDVVKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPYKFIPTLLADYPEYFQVC+VR
Sbjct: 121 SQPHRDDVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPTLLADYPEYFQVCSVR 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DCSTGEQTLALE+LSWR++LS+SELK+R   +GN W R G HIAFPMS PRGFD EKKV+
Sbjct: 181 DCSTGEQTLALELLSWREDLSVSELKKRHSLEGNGWRRNGIHIAFPMSFPRGFDLEKKVL 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQRLPYISPYENAFHLAPNSDQAEKWT AVLHELLYL+VSKKTEKENI CLGEY+
Sbjct: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENISCLGEYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYI LMNK V
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIRLMNKAV 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           RRPRP IILASSR KRQNNP +NKE
Sbjct: 361 RRPRPRIILASSRDKRQNNPSANKE 385

BLAST of MS012533 vs. ExPASy TrEMBL
Match: A0A5A7T0U1 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00230 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.7e-189
Identity = 321/385 (83.38%), Postives = 359/385 (93.25%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MIL+ TRIP+R +Q R FVDAKVKWVRDPYLDFAVQREKNL+QVISLK++I+S+P  S+P
Sbjct: 1   MILQITRIPHRFTQIRAFVDAKVKWVRDPYLDFAVQREKNLKQVISLKNIIISSPLTSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSLLKQNLKVPTTT+SKFF+LYPSVF QFQPS GLHP VK+T QAL LHKEESTIHN
Sbjct: 61  LSSVSLLKQNLKVPTTTISKFFELYPSVFIQFQPSLGLHPHVKITSQALLLHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           S+PHRDDVVKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPY+FIPTLLADYP+YFQVC+V+
Sbjct: 121 SRPHRDDVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYRFIPTLLADYPDYFQVCSVK 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DC TGEQTLALE+LSWRK+L++SELK+RE  +GN  SRK N IAFPMS PRGFD +KKVM
Sbjct: 181 DCLTGEQTLALELLSWRKDLAVSELKKREYLEGNFGSRKRNRIAFPMSFPRGFDLQKKVM 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQ LPYISPYENAFHLAPNSDQAEKW VAVLHELLYL +SKKTEKENI+CLG+Y+
Sbjct: 241 NWVEEWQHLPYISPYENAFHLAPNSDQAEKWAVAVLHELLYLTISKKTEKENIYCLGDYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY+KDFL+EKHPLMGMRHQY+HLMNKV+
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYKKDFLLEKHPLMGMRHQYLHLMNKVI 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           R+PRPGIILASSRGKR+N   +NKE
Sbjct: 361 RKPRPGIILASSRGKRRNIALANKE 385

BLAST of MS012533 vs. ExPASy TrEMBL
Match: A0A1S3C4Q9 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103496453 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.7e-189
Identity = 321/385 (83.38%), Postives = 359/385 (93.25%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           MIL+ TRIP+R +Q R FVDAKVKWVRDPYLDFAVQREKNL+QVISLK++I+S+P  S+P
Sbjct: 1   MILQITRIPHRFTQIRAFVDAKVKWVRDPYLDFAVQREKNLKQVISLKNIIISSPLTSVP 60

Query: 61  LSSVSLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHN 120
           LSSVSLLKQNLKVPTTT+SKFF+LYPSVF QFQPS GLHP VK+T QAL LHKEESTIHN
Sbjct: 61  LSSVSLLKQNLKVPTTTISKFFELYPSVFIQFQPSLGLHPHVKITSQALLLHKEESTIHN 120

Query: 121 SQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVR 180
           S+PHRDDVVKRLAKLLMLTGAG+LPLYVIE+LQWDLGLPY+FIPTLLADYP+YFQVC+V+
Sbjct: 121 SRPHRDDVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYRFIPTLLADYPDYFQVCSVK 180

Query: 181 DCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVM 240
           DC TGEQTLALE+LSWRK+L++SELK+RE  +GN  SRK N IAFPMS PRGFD +KKVM
Sbjct: 181 DCLTGEQTLALELLSWRKDLAVSELKKREYLEGNFGSRKRNRIAFPMSFPRGFDLQKKVM 240

Query: 241 NWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYM 300
           NWVEEWQ LPYISPYENAFHLAPNSDQAEKW VAVLHELLYL +SKKTEKENI+CLG+Y+
Sbjct: 241 NWVEEWQHLPYISPYENAFHLAPNSDQAEKWAVAVLHELLYLTISKKTEKENIYCLGDYL 300

Query: 301 GFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVV 360
           GFGSRFKKA+VHHPGIFYVSNKIRTQTVVLREAY+KDFL+EKHPLMGMRHQY+HLMNKV+
Sbjct: 301 GFGSRFKKAIVHHPGIFYVSNKIRTQTVVLREAYKKDFLLEKHPLMGMRHQYLHLMNKVI 360

Query: 361 RRPRPGIILASSRGKRQNNPYSNKE 386
           R+PRPGIILASSRGKR+N   +NKE
Sbjct: 361 RKPRPGIILASSRGKRRNIALANKE 385

BLAST of MS012533 vs. TAIR 10
Match: AT4G24320.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 466.1 bits (1198), Expect = 2.8e-131
Identity = 241/380 (63.42%), Postives = 289/380 (76.05%), Query Frame = 0

Query: 1   MILRSTRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLP 60
           M   +T++   L Q R FV+A+VKWV D YLD AVQREKNL+QVISLK  IVS+PSKSLP
Sbjct: 1   MAAATTKLARALQQRRTFVNARVKWVSDHYLDEAVQREKNLKQVISLKDRIVSSPSKSLP 60

Query: 61  LSSVSLLKQ--NLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPL-VKLTPQALTLHKEEST 120
           LSS+SLLK   NL +   T + FFQ YPSVFT FQPSP  HPL V+LTPQALTLHKEE T
Sbjct: 61  LSSLSLLKPLVNLHI---TAAAFFQKYPSVFTTFQPSPS-HPLHVRLTPQALTLHKEEET 120

Query: 121 IHNSQPHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVC 180
           IH S P R+  V+RL K LMLTGAG LPLYV++R ++DLGLP  +I +L+ DYPEYF+V 
Sbjct: 121 IHLSPPQRNVTVQRLTKFLMLTGAGSLPLYVLDRFRFDLGLPRDYITSLIGDYPEYFEVT 180

Query: 181 TVRDCSTGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEK 240
            ++D  TGE+TLAL I S R  L +SE++RRE        +KG  I + M+ P+G++  K
Sbjct: 181 EIKDRLTGEKTLALTISSRRNNLPVSEMERREATIDGSRVKKGLRIRYSMNFPKGYELHK 240

Query: 241 KVMNWVEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLG 300
           +V NWVE+WQ LPYISPYENAFHL   SDQAEKW VAVLHELL+LLVSKKTE +N+ CLG
Sbjct: 241 RVKNWVEQWQNLPYISPYENAFHLGSYSDQAEKWAVAVLHELLFLLVSKKTETDNVICLG 300

Query: 301 EYMGFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMN 360
           EY+GFG RFKKALVHHPGIFY+S+KIRTQTVVLREAY K FL+EKHPLMG+RHQYI+LM+
Sbjct: 301 EYLGFGMRFKKALVHHPGIFYMSHKIRTQTVVLREAYHKVFLLEKHPLMGIRHQYIYLMS 360

Query: 361 KVVRRPRPGIILASSRGKRQ 378
           K  R  +   +    R  R+
Sbjct: 361 KSGRGKKRDYVHGVERSNRK 376

BLAST of MS012533 vs. TAIR 10
Match: AT1G71850.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 308.1 bits (788), Expect = 9.7e-84
Identity = 154/344 (44.77%), Postives = 234/344 (68.02%), Query Frame = 0

Query: 19  VDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQNLKVPTTTV 78
           V+   K+VRD  LD AV+REKNLR ++S+K LI S P+KS+P+S ++  K +L+VP   +
Sbjct: 25  VEDTYKFVRDRGLDHAVEREKNLRPLLSIKDLIRSEPAKSVPISVITSQKDSLRVPLRPI 84

Query: 79  SKFFQLYPSVFTQFQPSP-GLHPLVKLTPQALTLHKEESTIHNSQPHRDDVVKRLAKLLM 138
            +F + +PSVF +F P   G+HP + LTP+ L    +E  ++ S+ ++  +  RL KLLM
Sbjct: 85  -EFIRSFPSVFQEFLPGGIGIHPHISLTPEILNHDADEQLVYGSETYKQGLADRLLKLLM 144

Query: 139 LTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQV--CTVRDCSTGEQTLALEILS 198
           +    ++PL +++ L+WDLGLP  ++ T++ ++P+YF+V    +R CS GE    LE++ 
Sbjct: 145 INRINKIPLEILDLLKWDLGLPKDYVETMVPEFPDYFRVIKSKLRGCS-GE----LELVC 204

Query: 199 WRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVMNWVEEWQRLPYISPY 258
           W  E ++S L+++          KG+ IAFPM    GF  +KK+  W+++WQ+LPYISPY
Sbjct: 205 WSNEHAVSVLEKKARTLRKGEYTKGSAIAFPMKFSNGFVVDKKMKKWIDDWQKLPYISPY 264

Query: 259 ENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKKALVHHPG 318
           ENA HL+  SD+++KW  AVLHE++ L VSKK EK+ I  LGE+MG  SRFK+ L +HPG
Sbjct: 265 ENALHLSATSDESDKWAAAVLHEIMNLFVSKKVEKDAILHLGEFMGLRSRFKRVLHNHPG 324

Query: 319 IFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKV 360
           IFY+S+K+RT TVVLR+ Y++  L+E + L+  R++Y+ LMN V
Sbjct: 325 IFYLSSKLRTHTVVLRDGYKRGMLIESNELVTSRNRYMKLMNTV 362

BLAST of MS012533 vs. TAIR 10
Match: AT5G48040.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.3e-64
Identity = 139/356 (39.04%), Postives = 212/356 (59.55%), Query Frame = 0

Query: 7   RIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSL 66
           R  +   Q  G V+ K+KWV+D  LD  V REK+LR V +L S+I ++P   LP+  +  
Sbjct: 21  RFDFFYQQRCGLVNVKLKWVKDRELDAVVVREKHLRAVCNLVSVISASPDLRLPIFKLLP 80

Query: 67  LKQNLKVP-TTTVSKFFQLYPSVFTQ--FQPSPGLH-PLVKLTPQALTLHKEESTIHNSQ 126
            +  L +P    +S F + YP++F +  +  S G   P   LT + + L+ EE  +  S+
Sbjct: 81  HRGQLGLPQELKLSAFIRRYPNIFVEHCYWDSAGTSVPCFGLTRETIDLYYEEVDV--SR 140

Query: 127 PHRDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDC 186
            +  DV+ RL KLLMLT    L L+ I+ L+WDLGLPY +  +L+  +P+ F +  +   
Sbjct: 141 VNERDVLVRLCKLLMLTCERTLSLHSIDHLRWDLGLPYDYRDSLITKHPDLFSLVKLSSD 200

Query: 187 STGEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVMNW 246
             G     L+++ W + L++S+++ RE   GN        +AFP+   RGF  ++K + W
Sbjct: 201 LDG-----LKLIHWDEHLAVSQMQLRE-DVGN-----DERMAFPVKFTRGFGLKRKSIEW 260

Query: 247 VEEWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGF 306
           ++EWQRLPY SPY +A HL P +D +EK  V V HELL+L + KKTE++N+  L +    
Sbjct: 261 LQEWQRLPYTSPYVDASHLDPRTDLSEKRNVGVFHELLHLTIGKKTERKNVSNLRKPFAL 320

Query: 307 GSRFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNK 359
             +F K    HPGIFY+S K  TQTV+LREAY +  L+EKHPL+ +R ++ ++MN+
Sbjct: 321 PQKFTKVFERHPGIFYISMKCDTQTVILREAYDRRHLIEKHPLVEVREKFANMMNE 363

BLAST of MS012533 vs. TAIR 10
Match: AT3G58520.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-54
Identity = 126/345 (36.52%), Postives = 199/345 (57.68%), Query Frame = 0

Query: 22  KVKWVRDPYLDFAVQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQNLKVPTTTVSKF 81
           ++KWV++  LD  +  E +L+    LK  I  +P+  L   SV+  ++ L + T  V +F
Sbjct: 32  RLKWVKNKNLDHVIDTETDLKAACILKDAIKRSPTGFLTAKSVADWQKLLGL-TVPVLRF 91

Query: 82  FQLYPSVFTQFQPSP-GLHPLVKLTPQALTLHKEESTIHNSQPHRDDVVKRLAKLLMLTG 141
            + YP++F +F  +     P  KLT  AL L  +E  IH  Q H  D V+RL ++LM+  
Sbjct: 92  LRRYPTLFHEFPHARYASLPCFKLTDTALMLDSQEEIIH--QSHEADTVERLCRVLMMMR 151

Query: 142 AGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCSTGEQTLALEILSWRKEL 201
           +  + L  +  L++DLGLP  +  TL+  YP++F  C V+  S G     L+++ WR E 
Sbjct: 152 SKTVSLRSLHSLKFDLGLPDNYEKTLVMKYPDHF--CFVK-ASNGNP--CLKLVKWRDEF 211

Query: 202 SISELKRRECPDGNV---------WSRKGNHIAFPMSLPRGFDFEKKVMNWVEEWQRLPY 261
           + S L++R   D +V         + R  + + FPMS PRG+  +KKV  W++E+Q+LPY
Sbjct: 212 AFSALQKRN-EDNDVSGEESRYREFKRGQSTLTFPMSFPRGYGAQKKVKAWMDEFQKLPY 271

Query: 262 ISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKKALV 321
           ISPY++  ++ P SD  EK  VAVLHELL L + KKT++  +  +   +    +F +   
Sbjct: 272 ISPYDDPSNIDPESDLMEKRAVAVLHELLSLTIHKKTKRNYLRSMRAELNIPHKFTRLFT 331

Query: 322 HHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLM 357
            +PGIFY+S K +T TV+L+E YR+  LV+ HPL  +R ++ H+M
Sbjct: 332 RYPGIFYLSLKCKTTTVILKEGYRRGKLVDPHPLTRLRDKFYHVM 367

BLAST of MS012533 vs. TAIR 10
Match: AT2G39120.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.8e-54
Identity = 122/347 (35.16%), Postives = 194/347 (55.91%), Query Frame = 0

Query: 12  LSQTRGFVDAKVKWVRDPYLDFA--VQREKNLRQVISLKSLIVSNPSKSLPLSSVSLLKQ 71
           L+Q R +VD  +KW RDPY D    + R   L+ V+SLK+ IV  P++ +P+S++S   +
Sbjct: 18  LTQKRTYVDVYMKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTR 77

Query: 72  NLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHNSQPHRDDVV 131
              V +T ++ F + +PS+F +F       P  +LTP+A  L ++E  ++  Q   DD+ 
Sbjct: 78  QFDV-STKIAHFLRKFPSIFEEFVGPEYNLPWFRLTPEATELDRQERVVY--QTSADDLR 137

Query: 132 KRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCSTGEQTL 191
            RL KL++++    LPL +++ ++W LGLP  ++     +    F+   + D   G + L
Sbjct: 138 DRLKKLILMSKDNVLPLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMED---GVKGL 197

Query: 192 ALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPRGFDFEKKVMNWVEEWQRL 251
           A++     K LS+ +    +   G V       I FP+   +G     K+ +W+ E+Q+L
Sbjct: 198 AVDYNGGDKVLSVLQKNAMKKRRGEV---SLEEIEFPLFPSKGCRLRVKIEDWLMEFQKL 257

Query: 252 PYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYMGFGSRFKKA 311
           PY+SPY++   L P+SD AEK  V  LHELL L V    E++ + CL ++ G   +  KA
Sbjct: 258 PYVSPYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHKA 317

Query: 312 LVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLM 357
              HP IFY+S K +T T +LRE YR    VE HP++G+R +YI LM
Sbjct: 318 FERHPQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKKYIQLM 355