MS012520 (gene) Bitter gourd (TR) v1

Overview
NameMS012520
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMagnesium transporter
Locationscaffold63: 1272506 .. 1277110 (+)
RNA-Seq ExpressionMS012520
SyntenyMS012520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAAGAAGAAGGCTGGAGGAGCGAGGCTGTGGATGCGTCTCGACAGATGGGGGCAGTCGGAGCTTGTAGAGTGGGACAAGAGCGCTATAATACGGCGTGCTGCAATTCCAGCTAGGGATTTGAGGATCCTTGGTCCTGTTTTCTCCCACTCCTCTAACATCCTTGGTAAATTTTCATCCTCCGCTGCAACCAGTTTTAGGAACTATAATTTTAACTTTGTAGAAGTGATCTTGCTCATCTTTGTTTCTTGTAACTGATTTTTTGAAATTATAGCAAAAACTTGTAAGGTTGTAGGGTTGTAGGAGTCGTGGTCACCAAATGCTAAATAGGTCGTTTTTTCACTATGAGAAAAAATGTTTAGTTGGTTTTGTCTGCTTCAATTGAGAGAGGAACCTGGAGAAATAGCTCTAGTTTGAAGAGATAAGGTCGTGTGCAATTTTTTTTGACATTCCATCTCAAATAGTATCAAGTATATTCGTGTTCTTTGTTCATTTTAGGAAACCATGAACGGCGAGTGGTTTTTAGTAGATTCTCATTTGTTTCTTTTATAGTGCGAAATGCATGTTGCATATGTTCTTGATTTTCCTGCTGTTGTTTTCAATGTATATTTTTTTGAGTTTAAGTTGTGGAACTATGAAGTCAGGGTTGAAATTGACAATTCATGAATGTTTATCACCAAGTGAAATCTAGAACAATCTTAAAAAAAAAAAAAAAAAAAAAATTTTGAACTATTTTCTCTTTGGTATCCTTGCAGCCAGGGAAAAGGCCATGGTTGTCAATTTGGAGTTTATAAAAGCTCTAGTTACTGCTGAAGAAGTCCTGCTACTTGACCCTCTTCGCCAAGAGGTTATTCCATTTGTGGATCAGCTAAGGAAGCTACTTGCTAAGAATAGTTCATCTCAGACTCAGGGCCATGGAGATGGAACTGTGGATGAACGTGAAAATGATGTGAATGTGTCGAGAGGAGGAAAATGGTTGCCAGTTTCGGAAGCTGTTGAAGGTGAGCAGTATGAGCTCCCGTTTGAGTTTCAGGTTCTGGAAAATGCGTTGGAGGTTGTGTGTATGTACTTAGATTCCAGTGTGGCAGACCTTGAGAGAGATGCCTATCCTGTCCTGGATGAACTGGCTAGGAATGTCAGCACCAAGAATCTTGAACATGTGAGGAGCTTGAAAAGTAATCTTACCCGTATTCTTGCACGTGTTCAGAAGGTGTGCTCGATTCCTACTCTTTCTACAAACTCTTCTAAAATGCTAATTTTAATTACTTATATTCAGCTTGAGATAGTTTTTTGTTTTGTTTGTTTTGGGGGGGGCGGTGGGTTGGTTCATGTATTACTTAAAAGTAGCTTATGGGAAAGCCTTTTTTTCCCTCCAAAATTTCTAACTGTGGTCCTAAATGCAATTAGACTTGGTGAAACGGTGATATGTGCAAAATTTTGAGGATTTGTGCTTGGCAATATATATGCGCCATGCAGGTTATAAAATATGTTCCTTACTATCAGGCATTAATGCTGTGTTTCTTAAAATCTCGTAGGTGCAACTCTGACATTCCCAAATCTAATGTATATGCTGTCTCGGATAAATACCCAATTCTAGTGATAAATGAACTATTAGATGAGCTCTGGGGTGACACTTTGTAAGCTGGATCTCAAGTCAGGTTATCATCAAATTTGGATCTGCTATGAGGACGTGCCCAAGATTGTCTTTCACACCCTTGAAGGTCACCATGAGTTCCAAGTGATTCACCTTGGGACAACAAATGCTCTGTCCTCATTTCAGCCCTTAATGAATACGATAATCAAATGTATAAATGGTCAAAATGTAGCTTTAGTTGTTGGAGTTGGAACTCTTAGGGCACGAAATGTCTCGACAAGGGAGTGTAAGTGGACCCCTTGTGCCAGCTAAAGTAATGTTGTGTGGCCCCTGGCTGAACTGTTAGATAAGGACCAATTTGGATGAAATATGCTTGCTGAGGCAGCCTTTGAACTTCATAAAGATGTACATGACTTTATCATTGACGTTGGCTCTCCTTGAGTTTGTTTGTAAAGTAGATAGAGCTTCAGGGTGTTGGATAATAGGAGTAATATCCAAGATCAAAGGCATATTGCTTACTAAAATCAAGTTCTATCGAACCAGGCAGGACAAAAATCCATTTATGAGAAAGAGTGTTGAATGTGGAACGTTTTCAGCTAACCAAGCCTTTTGGTCCTTTTCTTGAGACTATATCGTGTCAAGTTGCTATTGATTTAGTGAAAATGTCAGGGTTTGCTTGGGAGTAAGGGAACCCGATTTTATTTCTTTGCACTGGAGTGAAGCTCTAGTTGGGAGAGTTGGGCCCCTGGAAAGGTTGGAGAATATTGTATCTGTCTCACTGGCTGAATTCATTAGTTCCCTCTCTAATTCTGTTAAACTGTAGTCCTAAAACAGTTTGTCCCTTTGGCAATGTCTTCTTTACTTGTGGATCAGTAAAATAAAATGCTAATCATCTTAGAGTGAACCTCTCACCTTATTTGATGTTCTTTTATGAAAGGTCTGAGATGGATCTTTCACAACACATGAGAAGGTCTTGGTATAGAACACCAACCCACCCACCCCTCTTCTTTGGTGGATCATCTACTTAGTGGAATCTTTGGTTGCTGTTTGTATTGACCATATGTACATTTTGATTTCTCTGCTTTGCACGTGTAGATTTTGATGTATGCAAACTGTTCGGAAAGACAAATACCTGGAAAAGTGTCTTGGTTTCTAGTTGGGAAATTGTCGTGGAAAGCAACACTGAAGATAAACTTCGAAGTAAAAACCCTGTCATGACTCATTGCTATACCCTCAAGCTGGTGCACCCTTTGTAACGATGATGAGGAATGTTGAGATTACATTTTCATTCATTGGTACCTTGCCAGTTTTTTCTTTTACCTTCCTTTTCTCTTCTTTCAAGTGGCCTAGTCCATTTAACCGAGACATACAGTCCGGCTTCAACTCTCTCATGACTTACCACCATTTCTAGAAAAAGACTAGACACCTAATGCTCAGTTTTATCAGAAGCTTCCTTTTGATCACTTGGAAAGAAAAGAACTTACGTATTTTTTGGATTAAGAAAAGGAACAGCTCTCAACTTTTTACAGGATTCTTTTTGTAGTTGTCGCTGGGTGCAAAATGAGCCATTCTTTCTATACTTGTACTATTCTCTGATAAATGTGCTTATTGGAAGGATTTTCTTTAACTTCTTTTAGCCGCATTAAGGCTTTTCTCTCCCTCTTTTATTTCTGTCATCATTATTATTTTTAATAGAAAACGAAACTTGTATATTGATTTATGGAATGTCGTTTGAATCATATCCAAAATGAAAGCCAAAGAAAAGAGAAAAAGGGCTCATTTTTTTTTATCCAAAATGAAAATCAATGAAAAAAGGGCCCATCCCTTATGTGGGAAAAGCTTTCTGTAACCCCTTTTATGGGCCACTTTTGTAATTTCATATTATCAATGAAATTTTGTCTTTAACCAAAAAAGAAAAAATGGCTCATAGCTTTTATGATGGCAGGTGCGGGACGAGATAGAACATCTGTTAGACGATAATGAAGATATGGCACAATTATATTTGACAAGGAAATGGATGCAGAATCAACAATACGATGCTTTGCTGGGAGGCATGGCTTCCAACAGCACCACTGCCATTGTTCCCAATCTTCGTCGACTTAGTTCCACCAAGAGCGGAAGCGTGGTGACCAGCAATTATTTGGATGATAATGATGTTGAGGATCTGGAAATGTTGCTTGAGGCATATTTTATGCAGTTGGATGGTACACGTAACAGGATATTATCGGTAAGAGATAAGTTTCTTAATCATATCATGATAAAATTCCATAATTTAGGCAAGAACAAGAACAGCTTCTTTCTTTGAGTTTGTTTTGTTCGGGTTGAGGGGCATGCACGTTACAATGGTAATTGGTATGGGCCCTGTGGCTGAAGACAAGGTCAAGGAAGTGACTAGTTCTAGCCTTCTAGGAGTGCTTTTGGTCAAAATACTTTCTCAAATTTGAGAATAATATAAAGATGCTTTCGAGAAAAGTTAAAAGCATTTTGGCCCAAATGTTTGAAATTGGGAAGCAGGTCGAAAGCACTTTTAAGAGTGTATAATCAACTCTTAAAATCAAACATTCATTTATTATTAAGCACTCTTTACCAAAGTGCTTGCTGCTAAAAATACAAGCACACCGAATGCTGGAGATTATGAAATTATTCTGTCCCTAGGCGATTAGACTAGCATGGCGGGTTATGTTAACAGATTCAAGCGTGGAGTTTAAGACTTGTATCACTCATTTTTGTATGTGCAGGTCAGGGAATACATTGATGACACCGAGGATTACGTCAACATTCAACTCGACAACCAGAGAAATGAACTCATTCAGTTTCAACTGACCCTGACCATTGCTTCATTTGCCATAGCTGTTGAAACCTTAATTGCTGGGTTGTTCGGTATGAACATCCCTTGTACACTGTACAATATGGACGGGATATTCGAGTACTTCGTCGGAGGTACTTCAGCAGGTTGTTTGTTGATTTTCCTGCTTATTTTAGGGTATGCAAGATGGAAGAAGCTGCTTGGT

mRNA sequence

GTGAAGAAGAAGGCTGGAGGAGCGAGGCTGTGGATGCGTCTCGACAGATGGGGGCAGTCGGAGCTTGTAGAGTGGGACAAGAGCGCTATAATACGGCGTGCTGCAATTCCAGCTAGGGATTTGAGGATCCTTGGTCCTGTTTTCTCCCACTCCTCTAACATCCTTGCCAGGGAAAAGGCCATGGTTGTCAATTTGGAGTTTATAAAAGCTCTAGTTACTGCTGAAGAAGTCCTGCTACTTGACCCTCTTCGCCAAGAGGTTATTCCATTTGTGGATCAGCTAAGGAAGCTACTTGCTAAGAATAGTTCATCTCAGACTCAGGGCCATGGAGATGGAACTGTGGATGAACGTGAAAATGATGTGAATGTGTCGAGAGGAGGAAAATGGTTGCCAGTTTCGGAAGCTGTTGAAGGTGAGCAGTATGAGCTCCCGTTTGAGTTTCAGGTTCTGGAAAATGCGTTGGAGGTTGTGTGTATGTACTTAGATTCCAGTGTGGCAGACCTTGAGAGAGATGCCTATCCTGTCCTGGATGAACTGGCTAGGAATGTCAGCACCAAGAATCTTGAACATGTGAGGAGCTTGAAAAGTAATCTTACCCGTATTCTTGCACGTGTTCAGAAGGTGCGGGACGAGATAGAACATCTGTTAGACGATAATGAAGATATGGCACAATTATATTTGACAAGGAAATGGATGCAGAATCAACAATACGATGCTTTGCTGGGAGGCATGGCTTCCAACAGCACCACTGCCATTGTTCCCAATCTTCGTCGACTTAGTTCCACCAAGAGCGGAAGCGTGGTGACCAGCAATTATTTGGATGATAATGATGTTGAGGATCTGGAAATGTTGCTTGAGGCATATTTTATGCAGTTGGATGGTACACGTAACAGGATATTATCGGTCAGGGAATACATTGATGACACCGAGGATTACGTCAACATTCAACTCGACAACCAGAGAAATGAACTCATTCAGTTTCAACTGACCCTGACCATTGCTTCATTTGCCATAGCTGTTGAAACCTTAATTGCTGGGTTGTTCGGTATGAACATCCCTTGTACACTGTACAATATGGACGGGATATTCGAGTACTTCGTCGGAGGTACTTCAGCAGGTTGTTTGTTGATTTTCCTGCTTATTTTAGGGTATGCAAGATGGAAGAAGCTGCTTGGT

Coding sequence (CDS)

GTGAAGAAGAAGGCTGGAGGAGCGAGGCTGTGGATGCGTCTCGACAGATGGGGGCAGTCGGAGCTTGTAGAGTGGGACAAGAGCGCTATAATACGGCGTGCTGCAATTCCAGCTAGGGATTTGAGGATCCTTGGTCCTGTTTTCTCCCACTCCTCTAACATCCTTGCCAGGGAAAAGGCCATGGTTGTCAATTTGGAGTTTATAAAAGCTCTAGTTACTGCTGAAGAAGTCCTGCTACTTGACCCTCTTCGCCAAGAGGTTATTCCATTTGTGGATCAGCTAAGGAAGCTACTTGCTAAGAATAGTTCATCTCAGACTCAGGGCCATGGAGATGGAACTGTGGATGAACGTGAAAATGATGTGAATGTGTCGAGAGGAGGAAAATGGTTGCCAGTTTCGGAAGCTGTTGAAGGTGAGCAGTATGAGCTCCCGTTTGAGTTTCAGGTTCTGGAAAATGCGTTGGAGGTTGTGTGTATGTACTTAGATTCCAGTGTGGCAGACCTTGAGAGAGATGCCTATCCTGTCCTGGATGAACTGGCTAGGAATGTCAGCACCAAGAATCTTGAACATGTGAGGAGCTTGAAAAGTAATCTTACCCGTATTCTTGCACGTGTTCAGAAGGTGCGGGACGAGATAGAACATCTGTTAGACGATAATGAAGATATGGCACAATTATATTTGACAAGGAAATGGATGCAGAATCAACAATACGATGCTTTGCTGGGAGGCATGGCTTCCAACAGCACCACTGCCATTGTTCCCAATCTTCGTCGACTTAGTTCCACCAAGAGCGGAAGCGTGGTGACCAGCAATTATTTGGATGATAATGATGTTGAGGATCTGGAAATGTTGCTTGAGGCATATTTTATGCAGTTGGATGGTACACGTAACAGGATATTATCGGTCAGGGAATACATTGATGACACCGAGGATTACGTCAACATTCAACTCGACAACCAGAGAAATGAACTCATTCAGTTTCAACTGACCCTGACCATTGCTTCATTTGCCATAGCTGTTGAAACCTTAATTGCTGGGTTGTTCGGTATGAACATCCCTTGTACACTGTACAATATGGACGGGATATTCGAGTACTTCGTCGGAGGTACTTCAGCAGGTTGTTTGTTGATTTTCCTGCTTATTTTAGGGTATGCAAGATGGAAGAAGCTGCTTGGT

Protein sequence

VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAMVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG
Homology
BLAST of MS012520 vs. NCBI nr
Match: XP_022136744.1 (magnesium transporter MRS2-4 [Momordica charantia])

HSP 1 Score: 755.7 bits (1950), Expect = 1.9e-214
Identity = 391/392 (99.74%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 57  VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 116

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND
Sbjct: 117 MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 176

Query: 121 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 180
           VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA
Sbjct: 177 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 236

Query: 181 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 240
           RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL
Sbjct: 237 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 296

Query: 241 LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL 300
           LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL
Sbjct: 297 LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL 356

Query: 301 SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMD 360
           SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYN D
Sbjct: 357 SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNKD 416

Query: 361 GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG
Sbjct: 417 GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 448

BLAST of MS012520 vs. NCBI nr
Match: XP_023533619.1 (magnesium transporter MRS2-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.8 bits (1810), Expect = 3.3e-198
Identity = 364/392 (92.86%), Postives = 376/392 (95.92%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGA+LWMRLDRWGQSEL+EWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 63  VKKKAGGAKLWMRLDRWGQSELLEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 122

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLL DPLRQEVIPFVDQLRKLLA  SSS  QGHGDGTVDEREND
Sbjct: 123 MVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSS--QGHGDGTVDEREND 182

Query: 121 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 180
           VNVS GGKWLPVSEA EG QYELPFEFQVLENALEVVCMYLDSSV DLERDAYPVLDELA
Sbjct: 183 VNVSSGGKWLPVSEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLDELA 242

Query: 181 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 240
           RNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ DAL
Sbjct: 243 RNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQADAL 302

Query: 241 LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL 300
           LGG+ASNSTTAIVP+LRRLSST+SGS+VTSN LDDNDVEDLEMLLEAYFMQLDGTRNRIL
Sbjct: 303 LGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNRIL 362

Query: 301 SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMD 360
           SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAGLFGMNI CTLY+ +
Sbjct: 363 SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLYDQE 422

Query: 361 GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           GIFEYFVGGTS GCLLIF+LI GYA+WKKLLG
Sbjct: 423 GIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLG 452

BLAST of MS012520 vs. NCBI nr
Match: XP_022980784.1 (magnesium transporter MRS2-4-like [Cucurbita maxima])

HSP 1 Score: 693.0 bits (1787), Expect = 1.5e-195
Identity = 362/395 (91.65%), Postives = 375/395 (94.94%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGA+LWMRLDRWGQSEL+EWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 65  VKKKAGGAKLWMRLDRWGQSELLEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 124

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLL DPLRQEVIPFVDQLRKLLA  SSS  QGHGDG VDEREND
Sbjct: 125 MVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSS--QGHGDGAVDEREND 184

Query: 121 VNVSRGGKWLPVS---EAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 180
           VNVS GGKWLPVS   EA EG QYELPFEFQVLENALEVVCMYLDSSV DLE+DAYPVLD
Sbjct: 185 VNVSSGGKWLPVSEAAEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLEKDAYPVLD 244

Query: 181 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 240
           ELARNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 
Sbjct: 245 ELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQS 304

Query: 241 DALLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRN 300
           DALLGG+ASNSTTAIVP+LRRLSST+SGS+VTSN LDDNDVEDLEMLLEAYFMQLDGTRN
Sbjct: 305 DALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRN 364

Query: 301 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY 360
           RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAGLFGMNI CTLY
Sbjct: 365 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLY 424

Query: 361 NMDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + +GIFEYFVGGTS GCLLIF+LI GYA+WKKLLG
Sbjct: 425 DREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLG 457

BLAST of MS012520 vs. NCBI nr
Match: XP_022942922.1 (magnesium transporter MRS2-4-like [Cucurbita moschata])

HSP 1 Score: 692.6 bits (1786), Expect = 2.0e-195
Identity = 362/395 (91.65%), Postives = 375/395 (94.94%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGA+LWMRLDRWGQSEL+EWDK+AIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 63  VKKKAGGAKLWMRLDRWGQSELLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKA 122

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLL DPLRQEVIPFVDQLRKLLA  SSS  QGHGDGTVDEREND
Sbjct: 123 MVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSS--QGHGDGTVDEREND 182

Query: 121 VNVSRGGKWLPVS---EAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 180
           VNVS GGKWLPVS   EA EG  YELPFEFQVLENALEVVCMYLDSSV DLERDAYPVLD
Sbjct: 183 VNVSSGGKWLPVSEAAEAAEGVPYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLD 242

Query: 181 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 240
           ELARNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 
Sbjct: 243 ELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQA 302

Query: 241 DALLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRN 300
           DALLGG+ASNSTTAIVP+LRRLSST+SGS+VTSN LDDNDVEDLEMLLEAYFMQLDGTRN
Sbjct: 303 DALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRN 362

Query: 301 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY 360
           RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAGLFGMNI CTLY
Sbjct: 363 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLY 422

Query: 361 NMDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + +GIFEYFVGGTS GCLLIF+LI GYA+WKKLLG
Sbjct: 423 DREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLG 455

BLAST of MS012520 vs. NCBI nr
Match: KAG7031030.1 (Magnesium transporter MRS2-4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 689.9 bits (1779), Expect = 1.3e-194
Identity = 361/395 (91.39%), Postives = 374/395 (94.68%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGA+LWMRLDRWGQSEL+EWDK+AIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 65  VKKKAGGAKLWMRLDRWGQSELLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKA 124

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLL DPLRQEVIPFVDQLRKLLA  SSS  QGHGDGTVDEREN 
Sbjct: 125 MVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSS--QGHGDGTVDERENV 184

Query: 121 VNVSRGGKWLPVS---EAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 180
           VNVS GGKWLPVS   EA EG QYELPFEFQVLENALEVVCMYLDSSV DLERDAYPVLD
Sbjct: 185 VNVSSGGKWLPVSEAGEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLD 244

Query: 181 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 240
           ELARNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 
Sbjct: 245 ELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQA 304

Query: 241 DALLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRN 300
           DA LGG+ASNSTTAIVP+LRRLSST+SGS+VTSN LDDNDVEDLEMLLEAYFMQLDGTRN
Sbjct: 305 DAFLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRN 364

Query: 301 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY 360
           RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAGLFGMNI CTLY
Sbjct: 365 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLY 424

Query: 361 NMDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + +GIFEYFVGGTS GCLLIF+LI GYA+WKKLLG
Sbjct: 425 DREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLG 457

BLAST of MS012520 vs. ExPASy Swiss-Prot
Match: Q93ZD7 (Magnesium transporter MRS2-4 OS=Arabidopsis thaliana OX=3702 GN=MRS2-4 PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 6.5e-141
Identity = 271/394 (68.78%), Postives = 317/394 (80.46%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           KKK GGARLWMR DR G  E+VE DKS II+RA++PARDLRILGPVFSHSSNILAREKA+
Sbjct: 56  KKKTGGARLWMRFDRTGAMEVVECDKSTIIKRASVPARDLRILGPVFSHSSNILAREKAI 115

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDV 121
           VVNLE IKA+VTAEEVLLLDPLR EV+PFV++L++   + + +             EN +
Sbjct: 116 VVNLEVIKAIVTAEEVLLLDPLRPEVLPFVERLKQQFPQRNGN-------------ENAL 175

Query: 122 NVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELAR 181
             S   +     EA EG Q ELPFEFQVLE ALEVVC ++D SVA LE +A+PVLDEL +
Sbjct: 176 QASANVQSPLDPEAAEGLQSELPFEFQVLEIALEVVCSFVDKSVAALETEAWPVLDELTK 235

Query: 182 NVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALL 241
           NVST+NLE+VRSLKSNLTR+LARVQKVRDE+EHLLDDNEDMA LYLTRKW+QNQQ +A+L
Sbjct: 236 NVSTENLEYVRSLKSNLTRLLARVQKVRDELEHLLDDNEDMADLYLTRKWIQNQQTEAIL 295

Query: 242 GGMASNSTTA---IVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNR 301
            G ASNS         NL RL+S +S S+VTSN  +++DVEDLEMLLEAYFMQLDG RN+
Sbjct: 296 AGTASNSIALPAHNTSNLHRLTSNRSASMVTSN-TEEDDVEDLEMLLEAYFMQLDGMRNK 355

Query: 302 ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYN 361
           IL+VREYIDDTEDYVNIQLDNQRNELIQ QLTLTIASFAIA ETL+A LFGMNIPC LY+
Sbjct: 356 ILTVREYIDDTEDYVNIQLDNQRNELIQLQLTLTIASFAIAAETLLASLFGMNIPCPLYS 415

Query: 362 MDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + G+F YFV   +A C+++F++ LGYARWKKLLG
Sbjct: 416 IHGVFGYFVWSVTALCIVLFMVTLGYARWKKLLG 435

BLAST of MS012520 vs. ExPASy Swiss-Prot
Match: A3BV82 (Putative magnesium transporter MRS2-G OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-G PE=1 SV=2)

HSP 1 Score: 455.3 bits (1170), Expect = 7.0e-127
Identity = 258/402 (64.18%), Postives = 303/402 (75.37%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           V KK  GARLWMRLDRWG SE +  DK +IIRRA +P RDLRILGPVFS SS+ILAREKA
Sbjct: 76  VGKKKAGARLWMRLDRWGVSETLHLDKGSIIRRAGLPPRDLRILGPVFSDSSSILAREKA 135

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQ------LRKLLAKNSSSQTQGHGDGTV 120
           MV+NLEFI+A+VTA+E+LLLDPL  +VIPFV+Q      L+ L+  N       HG    
Sbjct: 136 MVINLEFIRAIVTADEILLLDPLTIDVIPFVEQLTHHLPLKNLVCGNGQPGGDDHG---- 195

Query: 121 DERENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYP 180
            E+ +D +    G  +P      G ++ELPFEFQVLE ALE VC   D +V+ LER A P
Sbjct: 196 -EKHDDSH----GDQVPRLNEATGAEHELPFEFQVLELALETVCSSFDVNVSGLERRATP 255

Query: 181 VLDELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQN 240
           VL+EL +NVST+NL+ VR+LKS+LTR+LA VQKVRDEIEHLLDDNEDMA LYLTRK +QN
Sbjct: 256 VLEELTKNVSTRNLDRVRTLKSDLTRLLAHVQKVRDEIEHLLDDNEDMAHLYLTRKQLQN 315

Query: 241 QQYDALLGGMASNSTTAIVPNLRRLSST--KSGSVVTSNYLDDNDVEDLEMLLEAYFMQL 300
           QQ +AL+   ASNS      +L RL+++  +S S+ TS +L DNDVEDLEMLLEAYFMQL
Sbjct: 316 QQVEALISSAASNSIVPGGTSLSRLNNSFRRSVSIATSMHL-DNDVEDLEMLLEAYFMQL 375

Query: 301 DGTRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNI 360
           DG RNRILSVREYIDDTEDYVNIQLDNQRNELIQ QLTLTIASF IAV T IAG F MNI
Sbjct: 376 DGIRNRILSVREYIDDTEDYVNIQLDNQRNELIQLQLTLTIASFGIAVNTFIAGAFAMNI 435

Query: 361 PCTLYNMD--GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
              LY++D    F  FVGGTS+GC +I +++L YARWKKLLG
Sbjct: 436 QSKLYSIDDGSFFWPFVGGTSSGCFMICIVLLWYARWKKLLG 467

BLAST of MS012520 vs. ExPASy Swiss-Prot
Match: A2Z9W7 (Putative magnesium transporter MRS2-G OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-G PE=3 SV=2)

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-126
Identity = 258/402 (64.18%), Postives = 302/402 (75.12%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           V KK  GARLWMRLDRWG SE +  DK +IIRRA +P RDLRILGPVFS SS+ILAREKA
Sbjct: 76  VGKKKAGARLWMRLDRWGVSETLHLDKGSIIRRAGLPPRDLRILGPVFSDSSSILAREKA 135

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQ------LRKLLAKNSSSQTQGHGDGTV 120
           MV+NLEFI+A+VTA+E+LLLDPL  +VIPFV+Q      L+ L+  N       HG    
Sbjct: 136 MVINLEFIRAIVTADEILLLDPLTIDVIPFVEQLTHHLPLKNLVCGNGQPGGDDHG---- 195

Query: 121 DERENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYP 180
            E+ +D      G  +P      G ++ELPFEFQVLE ALE VC   D +V+ LER A P
Sbjct: 196 -EKHDD----SPGDQVPRLNEATGAEHELPFEFQVLELALETVCSSFDVNVSGLERRATP 255

Query: 181 VLDELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQN 240
           VL+EL +NVST+NL+ VR+LKS+LTR+LA VQKVRDEIEHLLDDNEDMA LYLTRK +QN
Sbjct: 256 VLEELTKNVSTRNLDRVRTLKSDLTRLLAHVQKVRDEIEHLLDDNEDMAHLYLTRKQLQN 315

Query: 241 QQYDALLGGMASNSTTAIVPNLRRLSST--KSGSVVTSNYLDDNDVEDLEMLLEAYFMQL 300
           QQ +AL+   ASNS      +L RL+++  +S S+ TS +L DNDVEDLEMLLEAYFMQL
Sbjct: 316 QQVEALISSAASNSIVPGGTSLSRLNNSFRRSVSIATSMHL-DNDVEDLEMLLEAYFMQL 375

Query: 301 DGTRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNI 360
           DG RNRILSVREYIDDTEDYVNIQLDNQRNELIQ QLTLTIASF IAV T IAG F MNI
Sbjct: 376 DGIRNRILSVREYIDDTEDYVNIQLDNQRNELIQLQLTLTIASFGIAVNTFIAGAFAMNI 435

Query: 361 PCTLYNMD--GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
              LY++D    F  FVGGTS+GC +I +++L YARWKKLLG
Sbjct: 436 QSKLYSIDDGSFFWPFVGGTSSGCFMICIVLLWYARWKKLLG 467

BLAST of MS012520 vs. ExPASy Swiss-Prot
Match: A2XCA0 (Putative magnesium transporter MRS2-H OS=Oryza sativa subsp. indica OX=39946 GN=MRS2-H PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.3e-92
Identity = 206/399 (51.63%), Postives = 263/399 (65.91%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           K+KA   RLWMRLDR G  E++  DKS + RR+ +PARDLR+LGP+ S S +ILAREKAM
Sbjct: 55  KRKAANTRLWMRLDRRGGCEMILCDKSFVARRSGLPARDLRVLGPLLSRSPSILAREKAM 114

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNS----SSQTQGHGDGTVDER 121
           V+NLEF++A+VTA+EVL+L+PL QEV+PFV++LRK     S       T  H +    E 
Sbjct: 115 VINLEFVRAIVTADEVLVLEPLAQEVLPFVEKLRKHFPLKSLDVDDVSTHMHTENQDGEL 174

Query: 122 ENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 181
             DV+             VEG  +ELPFEFQVL+ ALE VC+  +S+++DL R A  VLD
Sbjct: 175 AQDVSCYE----------VEGANHELPFEFQVLDFALEAVCLSYNSTISDLNRSAIAVLD 234

Query: 182 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 241
           +L ++VST+NLE VRSLKS+LTR+LA VQKVRDE+EH+LDDNE MA L   RK     Q 
Sbjct: 235 DLMKSVSTRNLERVRSLKSSLTRLLASVQKVRDEVEHILDDNEAMAHLCTARK--TKGQK 294

Query: 242 DALLGGMASNSTTAIVPNLRRL---SSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDG 301
           D L         T + P  R     SS ++ + + +    D+D   L+MLLEAYF QLDG
Sbjct: 295 DLL--------NTILFPETRLCRTHSSIENSTGIRTCVPSDSDAHILDMLLEAYFKQLDG 354

Query: 302 TRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPC 361
            RNRI  VR+YI DTEDY++IQLDN+RNEL+  QLTL IASF IA+ T IA  F MNIP 
Sbjct: 355 IRNRIFLVRQYIVDTEDYISIQLDNKRNELLGLQLTLIIASFGIAINTFIAAAFAMNIPH 414

Query: 362 TLYN-MDGI-FEYFVGGTSAGCLLIFLLILGYARWKKLL 392
             Y+ + G+ F  FVG TS  C+ I +L+  YA   +LL
Sbjct: 415 RGYHFVIGVPFGQFVGATSFLCMSIVILLFTYAWRNRLL 433

BLAST of MS012520 vs. ExPASy Swiss-Prot
Match: Q10S25 (Putative magnesium transporter MRS2-H OS=Oryza sativa subsp. japonica OX=39947 GN=MRS2-H PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-90
Identity = 204/399 (51.13%), Postives = 261/399 (65.41%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           K+KA   RLWMRLDR G  E++  DKS + RR+ +PARDLR+L P+ S S +ILAREKAM
Sbjct: 55  KRKAANTRLWMRLDRRGGCEMILCDKSFVARRSGLPARDLRVLSPLLSRSPSILAREKAM 114

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNS----SSQTQGHGDGTVDER 121
           V+NLEF++A+VTA+EVL+L+PL QEV+PFV++LRK     S       T  H +    E 
Sbjct: 115 VINLEFVRAIVTADEVLVLEPLAQEVLPFVEKLRKHFPLKSLDVDDVSTHMHTENQDGEL 174

Query: 122 ENDVNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 181
             DV+             VEG  +ELPFEFQVL+ ALE VC+  +S+++DL R A  VLD
Sbjct: 175 AQDVSCYE----------VEGANHELPFEFQVLDFALEAVCLSYNSTISDLNRSAIAVLD 234

Query: 182 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 241
           +L ++VST+NLE V SLKS+LTR+LA VQKVRDE+EH+LDDNE MA L   RK     Q 
Sbjct: 235 DLMKSVSTRNLERVWSLKSSLTRLLASVQKVRDEVEHILDDNEAMAHLCTARK--TKGQK 294

Query: 242 DALLGGMASNSTTAIVPNLRRL---SSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDG 301
           D L         T + P  R     SS ++ + + +    D+D   L+MLLEAYF QLDG
Sbjct: 295 DLL--------NTILFPETRLCRTHSSIENSTGIRTCVPSDSDAHILDMLLEAYFKQLDG 354

Query: 302 TRNRILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPC 361
            RNRI  VR+YI DTEDY++IQLDN+RNEL+  QLTL IASF IA+ T IA  F MNIP 
Sbjct: 355 IRNRIFLVRQYIVDTEDYISIQLDNKRNELLGLQLTLIIASFGIAINTFIAAAFAMNIPH 414

Query: 362 TLYN-MDGI-FEYFVGGTSAGCLLIFLLILGYARWKKLL 392
             Y+ + G+ F  FVG TS  C+ I +L+  YA   +LL
Sbjct: 415 RGYHFVIGVPFGQFVGATSFLCMSIVILLFTYAWRNRLL 433

BLAST of MS012520 vs. ExPASy TrEMBL
Match: A0A6J1C580 (Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111008374 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 9.3e-215
Identity = 391/392 (99.74%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 57  VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 116

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND
Sbjct: 117 MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 176

Query: 121 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 180
           VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA
Sbjct: 177 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 236

Query: 181 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 240
           RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL
Sbjct: 237 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 296

Query: 241 LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL 300
           LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL
Sbjct: 297 LGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRIL 356

Query: 301 SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMD 360
           SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYN D
Sbjct: 357 SVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNKD 416

Query: 361 GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG
Sbjct: 417 GIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 448

BLAST of MS012520 vs. ExPASy TrEMBL
Match: A0A6J1J073 (Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111480067 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.4e-196
Identity = 362/395 (91.65%), Postives = 375/395 (94.94%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGA+LWMRLDRWGQSEL+EWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 65  VKKKAGGAKLWMRLDRWGQSELLEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 124

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLL DPLRQEVIPFVDQLRKLLA  SSS  QGHGDG VDEREND
Sbjct: 125 MVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSS--QGHGDGAVDEREND 184

Query: 121 VNVSRGGKWLPVS---EAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 180
           VNVS GGKWLPVS   EA EG QYELPFEFQVLENALEVVCMYLDSSV DLE+DAYPVLD
Sbjct: 185 VNVSSGGKWLPVSEAAEAAEGVQYELPFEFQVLENALEVVCMYLDSSVEDLEKDAYPVLD 244

Query: 181 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 240
           ELARNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 
Sbjct: 245 ELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQS 304

Query: 241 DALLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRN 300
           DALLGG+ASNSTTAIVP+LRRLSST+SGS+VTSN LDDNDVEDLEMLLEAYFMQLDGTRN
Sbjct: 305 DALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRN 364

Query: 301 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY 360
           RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAGLFGMNI CTLY
Sbjct: 365 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLY 424

Query: 361 NMDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + +GIFEYFVGGTS GCLLIF+LI GYA+WKKLLG
Sbjct: 425 DREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLG 457

BLAST of MS012520 vs. ExPASy TrEMBL
Match: A0A6J1FRL4 (Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111447807 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 9.6e-196
Identity = 362/395 (91.65%), Postives = 375/395 (94.94%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGGA+LWMRLDRWGQSEL+EWDK+AIIRRAAIPARDLRILGPVFSHSSNILAREKA
Sbjct: 63  VKKKAGGAKLWMRLDRWGQSELLEWDKNAIIRRAAIPARDLRILGPVFSHSSNILAREKA 122

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLL DPLRQEVIPFVDQLRKLLA  SSS  QGHGDGTVDEREND
Sbjct: 123 MVVNLEFIKAIVTAEEVLLFDPLRQEVIPFVDQLRKLLATKSSS--QGHGDGTVDEREND 182

Query: 121 VNVSRGGKWLPVS---EAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLD 180
           VNVS GGKWLPVS   EA EG  YELPFEFQVLENALEVVCMYLDSSV DLERDAYPVLD
Sbjct: 183 VNVSSGGKWLPVSEAAEAAEGVPYELPFEFQVLENALEVVCMYLDSSVEDLERDAYPVLD 242

Query: 181 ELARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQY 240
           ELARNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ 
Sbjct: 243 ELARNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQA 302

Query: 241 DALLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRN 300
           DALLGG+ASNSTTAIVP+LRRLSST+SGS+VTSN LDDNDVEDLEMLLEAYFMQLDGTRN
Sbjct: 303 DALLGGLASNSTTAIVPHLRRLSSTRSGSMVTSNLLDDNDVEDLEMLLEAYFMQLDGTRN 362

Query: 301 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY 360
           RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIA+ETLIAGLFGMNI CTLY
Sbjct: 363 RILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAIETLIAGLFGMNIHCTLY 422

Query: 361 NMDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + +GIFEYFVGGTS GCLLIF+LI GYA+WKKLLG
Sbjct: 423 DREGIFEYFVGGTSVGCLLIFVLIFGYAKWKKLLG 455

BLAST of MS012520 vs. ExPASy TrEMBL
Match: A0A1S3C2A5 (Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103496094 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 353/393 (89.82%), Postives = 367/393 (93.38%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGG+RLWMRLDRWGQSEL+EWDK+AIIRR  IPARD+RILGPVFSHSSNILAREKA
Sbjct: 61  VKKKAGGSRLWMRLDRWGQSELLEWDKNAIIRRVGIPARDMRILGPVFSHSSNILAREKA 120

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLLLDPLRQEVIPFVDQLRKLLAK  SSQ            END
Sbjct: 121 MVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVDQLRKLLAKTGSSQL-----------END 180

Query: 121 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 180
            NVSRGGKWLPVSEA EGEQYELPFEFQVLENALEVVC+YLDSSVADLERDAYPVLDELA
Sbjct: 181 GNVSRGGKWLPVSEAAEGEQYELPFEFQVLENALEVVCLYLDSSVADLERDAYPVLDELA 240

Query: 181 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 240
           RNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ+DAL
Sbjct: 241 RNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQFDAL 300

Query: 241 L-GGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRI 300
           + GG ASNSTTA+VP+LRRLSS +SGSVVTSN LDDNDVEDLEMLLEAYFMQLDGTRNRI
Sbjct: 301 MGGGGASNSTTAVVPHLRRLSSNRSGSVVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNRI 360

Query: 301 LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNM 360
           LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY  
Sbjct: 361 LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYET 420

Query: 361 DGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           DG+F YFVGGTSAGCLL+FLLILGYA+WKKLLG
Sbjct: 421 DGVFGYFVGGTSAGCLLLFLLILGYAKWKKLLG 442

BLAST of MS012520 vs. ExPASy TrEMBL
Match: A0A5D3CIR6 (Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00310 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 353/393 (89.82%), Postives = 367/393 (93.38%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKKAGG+RLWMRLDRWGQSEL+EWDK+AIIRR  IPARD+RILGPVFSHSSNILAREKA
Sbjct: 61  VKKKAGGSRLWMRLDRWGQSELLEWDKNAIIRRVGIPARDMRILGPVFSHSSNILAREKA 120

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDEREND 120
           MVVNLEFIKA+VTAEEVLLLDPLRQEVIPFVDQLRKLLAK  SSQ            END
Sbjct: 121 MVVNLEFIKAIVTAEEVLLLDPLRQEVIPFVDQLRKLLAKTGSSQL-----------END 180

Query: 121 VNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELA 180
            NVSRGGKWLPVSEA EGEQYELPFEFQVLENALEVVC+YLDSSVADLERDAYPVLDELA
Sbjct: 181 GNVSRGGKWLPVSEAAEGEQYELPFEFQVLENALEVVCLYLDSSVADLERDAYPVLDELA 240

Query: 181 RNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDAL 240
           RNVSTKNLE VRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQ+DAL
Sbjct: 241 RNVSTKNLERVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQFDAL 300

Query: 241 L-GGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRI 300
           + GG ASNSTTA+VP+LRRLSS +SGSVVTSN LDDNDVEDLEMLLEAYFMQLDGTRNRI
Sbjct: 301 MGGGGASNSTTAVVPHLRRLSSNRSGSVVTSNLLDDNDVEDLEMLLEAYFMQLDGTRNRI 360

Query: 301 LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNM 360
           LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLY  
Sbjct: 361 LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYET 420

Query: 361 DGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           DG+F YFVGGTSAGCLL+FLLILGYA+WKKLLG
Sbjct: 421 DGVFGYFVGGTSAGCLLLFLLILGYAKWKKLLG 442

BLAST of MS012520 vs. TAIR 10
Match: AT3G58970.1 (magnesium transporter 6 )

HSP 1 Score: 501.9 bits (1291), Expect = 4.6e-142
Identity = 271/394 (68.78%), Postives = 317/394 (80.46%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           KKK GGARLWMR DR G  E+VE DKS II+RA++PARDLRILGPVFSHSSNILAREKA+
Sbjct: 56  KKKTGGARLWMRFDRTGAMEVVECDKSTIIKRASVPARDLRILGPVFSHSSNILAREKAI 115

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDV 121
           VVNLE IKA+VTAEEVLLLDPLR EV+PFV++L++   + + +             EN +
Sbjct: 116 VVNLEVIKAIVTAEEVLLLDPLRPEVLPFVERLKQQFPQRNGN-------------ENAL 175

Query: 122 NVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELAR 181
             S   +     EA EG Q ELPFEFQVLE ALEVVC ++D SVA LE +A+PVLDEL +
Sbjct: 176 QASANVQSPLDPEAAEGLQSELPFEFQVLEIALEVVCSFVDKSVAALETEAWPVLDELTK 235

Query: 182 NVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALL 241
           NVST+NLE+VRSLKSNLTR+LARVQKVRDE+EHLLDDNEDMA LYLTRKW+QNQQ +A+L
Sbjct: 236 NVSTENLEYVRSLKSNLTRLLARVQKVRDELEHLLDDNEDMADLYLTRKWIQNQQTEAIL 295

Query: 242 GGMASNSTTA---IVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNR 301
            G ASNS         NL RL+S +S S+VTSN  +++DVEDLEMLLEAYFMQLDG RN+
Sbjct: 296 AGTASNSIALPAHNTSNLHRLTSNRSASMVTSN-TEEDDVEDLEMLLEAYFMQLDGMRNK 355

Query: 302 ILSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYN 361
           IL+VREYIDDTEDYVNIQLDNQRNELIQ QLTLTIASFAIA ETL+A LFGMNIPC LY+
Sbjct: 356 ILTVREYIDDTEDYVNIQLDNQRNELIQLQLTLTIASFAIAAETLLASLFGMNIPCPLYS 415

Query: 362 MDGIFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
           + G+F YFV   +A C+++F++ LGYARWKKLLG
Sbjct: 416 IHGVFGYFVWSVTALCIVLFMVTLGYARWKKLLG 435

BLAST of MS012520 vs. TAIR 10
Match: AT4G28580.1 (magnesium transport 5 )

HSP 1 Score: 334.0 bits (855), Expect = 1.7e-91
Identity = 193/390 (49.49%), Postives = 260/390 (66.67%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           KK+ GG  LW R DR G  E+   DKS II R+++ A+DLR     FSHSS ILAREKA+
Sbjct: 46  KKRRGGVCLWTRFDRTGFMEVAGCDKSTIIERSSVSAKDLR---TAFSHSSKILAREKAI 105

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDV 121
           V+NLE IKA++T+E+V+LLD LR EV+   D+L+             H     D  EN +
Sbjct: 106 VLNLEVIKAVITSEQVMLLDSLRPEVLTLTDRLK-------------HHFPRKDGPENIL 165

Query: 122 NVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELAR 181
             S  G         EG + +LPFEF+VLE A EV C ++DS+V DLE  A+ +LDEL +
Sbjct: 166 QASSHGH---QEGGEEGLKSKLPFEFRVLEIAFEVFCSFVDSNVVDLETQAWSILDELTK 225

Query: 182 NVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALL 241
            VS +NL+ +RSLK++LT +LARVQKVRDEIEH LDD EDM  LYLTRKW+QNQQ +A  
Sbjct: 226 KVSNENLKDLRSLKTSLTHLLARVQKVRDEIEHFLDDKEDMEDLYLTRKWIQNQQTEA-- 285

Query: 242 GGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRILS 301
              ASNS  +  PNL+R +S +   + TS   +++D++D+EMLLEAYFMQL+G RN+IL 
Sbjct: 286 ---ASNSIVS-QPNLQRHTSNR---ISTSMVTEEDDIDDMEMLLEAYFMQLEGMRNKILL 345

Query: 302 VREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMDG 361
           ++E+ID TE YV I  +++RN LI   + + I ++AI   T++  LFGMNIP  LY+   
Sbjct: 346 MKEHIDSTEAYVKILQNSRRNGLIHLMMLVNIGNYAITAGTVVVNLFGMNIPIGLYSTPD 405

Query: 362 IFEYFVGGTSAGCLLIFLLILGYARWKKLL 392
           IF Y V    A C+++F++ +GYA+WKKLL
Sbjct: 406 IFGYVVWAVVALCIVLFIVTVGYAKWKKLL 407

BLAST of MS012520 vs. TAIR 10
Match: AT5G64560.1 (magnesium transporter 9 )

HSP 1 Score: 324.7 bits (831), Expect = 1.0e-88
Identity = 188/394 (47.72%), Postives = 262/394 (66.50%), Query Frame = 0

Query: 1   VKKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKA 60
           VKKK   A  W  +D  GQSE ++ DK  I+ R  I ARDLRIL P  S+ S IL RE+A
Sbjct: 18  VKKKTPQAS-WALIDATGQSEPLDVDKYEIMHRVQIHARDLRILDPNLSYPSTILGRERA 77

Query: 61  MVVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDG-TVDEREN 120
           +V+NLE IKA++T+EEVLL DP  + VIP V++LR+ L   ++S   G GDG  +   +N
Sbjct: 78  IVLNLEHIKAIITSEEVLLRDPSDENVIPVVEELRRRLPVGNASHNGGQGDGKEIAGAQN 137

Query: 121 DVNVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDEL 180
           D +               G++ E PFEF+ LE ALE +C +L +  A+LE  AYP LDEL
Sbjct: 138 DGDT--------------GDEDESPFEFRALEVALEAICSFLAARTAELETAAYPALDEL 197

Query: 181 ARNVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDA 240
              +S++NL+ VR LKS +TR+ ARVQKVRDE+E LLDD++DMA LYL+RK        +
Sbjct: 198 TSKISSRNLDRVRKLKSAMTRLTARVQKVRDELEQLLDDDDDMADLYLSRKLSSASSPIS 257

Query: 241 LLGGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRI 300
            +G     +T+   P +    S  S + + + + D+NDVE+LEMLLEAYFMQ+D T NR+
Sbjct: 258 SIGEPNWYTTS---PTIGSKISRASRASLATVHGDENDVEELEMLLEAYFMQIDSTLNRL 317

Query: 301 LSVREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNM 360
            ++REYIDDTEDY+NIQLDN RN+LIQ +L L+  +  +++ +L+AG+FGMNIP T  + 
Sbjct: 318 TTLREYIDDTEDYINIQLDNHRNQLIQLELVLSSGTVCLSMYSLVAGIFGMNIPYTWNDG 377

Query: 361 DG-IFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
            G +F+Y VG T   C+++F++I+ YAR+K L+G
Sbjct: 378 HGYMFKYVVGLTGTLCVVVFVIIMSYARYKGLVG 393

BLAST of MS012520 vs. TAIR 10
Match: AT5G09690.1 (magnesium transporter 7 )

HSP 1 Score: 300.4 bits (768), Expect = 2.1e-81
Identity = 175/392 (44.64%), Postives = 250/392 (63.78%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           +K +  +R W+ +D  GQ  +++ DK  I+ R  I ARDLRIL P   + S IL RE+A+
Sbjct: 20  RKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNLFYPSAILGRERAI 79

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDV 121
           V+NLE IKA++TAEEVL+ D   + VIP +++ ++ L   + +    HGDG +       
Sbjct: 80  VLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGNEAHGV-HGDGDL------- 139

Query: 122 NVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELAR 181
                           GE+ E PFEF+ LE ALE +C +L +   +LE+ AYP LDEL  
Sbjct: 140 ----------------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPALDELTL 199

Query: 182 NVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALL 241
            +S++NLE VR LKS +TR+ ARVQKVRDE+E LLDD+ DMA LYLTRK +      ++ 
Sbjct: 200 KISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASSSVSVS 259

Query: 242 GGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRILS 301
                  T+  + ++  +S     S+VT    D+ DVE+LEMLLEAYFMQ+D T N++  
Sbjct: 260 DEPIWYPTSPTIGSM--ISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDSTLNKLTE 319

Query: 302 VREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMDG 361
           +REYIDDTEDY+NIQLDN RN+LIQ +L L+  +  ++V ++IAG+FGMNIP T  +  G
Sbjct: 320 LREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDHG 379

Query: 362 -IFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
            IF++ V  T   C+++F++IL YAR++ L+G
Sbjct: 380 YIFKWVVSLTGTFCIVLFVIILSYARFRGLIG 385

BLAST of MS012520 vs. TAIR 10
Match: AT5G09690.2 (magnesium transporter 7 )

HSP 1 Score: 300.4 bits (768), Expect = 2.1e-81
Identity = 175/392 (44.64%), Postives = 250/392 (63.78%), Query Frame = 0

Query: 2   KKKAGGARLWMRLDRWGQSELVEWDKSAIIRRAAIPARDLRILGPVFSHSSNILAREKAM 61
           +K +  +R W+ +D  GQ  +++ DK  I+ R  I ARDLRIL P   + S IL RE+A+
Sbjct: 31  RKTSQLSRSWISIDATGQKTVLDVDKHVIMHRVQIHARDLRILDPNLFYPSAILGRERAI 90

Query: 62  VVNLEFIKALVTAEEVLLLDPLRQEVIPFVDQLRKLLAKNSSSQTQGHGDGTVDERENDV 121
           V+NLE IKA++TAEEVL+ D   + VIP +++ ++ L   + +    HGDG +       
Sbjct: 91  VLNLEHIKAIITAEEVLIRDSSDENVIPVLEEFQRRLPVGNEAHGV-HGDGDL------- 150

Query: 122 NVSRGGKWLPVSEAVEGEQYELPFEFQVLENALEVVCMYLDSSVADLERDAYPVLDELAR 181
                           GE+ E PFEF+ LE ALE +C +L +   +LE+ AYP LDEL  
Sbjct: 151 ----------------GEEDESPFEFRALEVALEAICSFLAARTTELEKFAYPALDELTL 210

Query: 182 NVSTKNLEHVRSLKSNLTRILARVQKVRDEIEHLLDDNEDMAQLYLTRKWMQNQQYDALL 241
            +S++NLE VR LKS +TR+ ARVQKVRDE+E LLDD+ DMA LYLTRK +      ++ 
Sbjct: 211 KISSRNLERVRKLKSAMTRLTARVQKVRDELEQLLDDDGDMADLYLTRKLVGASSSVSVS 270

Query: 242 GGMASNSTTAIVPNLRRLSSTKSGSVVTSNYLDDNDVEDLEMLLEAYFMQLDGTRNRILS 301
                  T+  + ++  +S     S+VT    D+ DVE+LEMLLEAYFMQ+D T N++  
Sbjct: 271 DEPIWYPTSPTIGSM--ISRASRVSLVTVRGDDETDVEELEMLLEAYFMQIDSTLNKLTE 330

Query: 302 VREYIDDTEDYVNIQLDNQRNELIQFQLTLTIASFAIAVETLIAGLFGMNIPCTLYNMDG 361
           +REYIDDTEDY+NIQLDN RN+LIQ +L L+  +  ++V ++IAG+FGMNIP T  +  G
Sbjct: 331 LREYIDDTEDYINIQLDNHRNQLIQLELMLSAGTVCVSVYSMIAGIFGMNIPNTWNHDHG 390

Query: 362 -IFEYFVGGTSAGCLLIFLLILGYARWKKLLG 393
            IF++ V  T   C+++F++IL YAR++ L+G
Sbjct: 391 YIFKWVVSLTGTFCIVLFVIILSYARFRGLIG 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136744.11.9e-21499.74magnesium transporter MRS2-4 [Momordica charantia][more]
XP_023533619.13.3e-19892.86magnesium transporter MRS2-4-like [Cucurbita pepo subsp. pepo][more]
XP_022980784.11.5e-19591.65magnesium transporter MRS2-4-like [Cucurbita maxima][more]
XP_022942922.12.0e-19591.65magnesium transporter MRS2-4-like [Cucurbita moschata][more]
KAG7031030.11.3e-19491.39Magnesium transporter MRS2-4, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
Q93ZD76.5e-14168.78Magnesium transporter MRS2-4 OS=Arabidopsis thaliana OX=3702 GN=MRS2-4 PE=2 SV=1[more]
A3BV827.0e-12764.18Putative magnesium transporter MRS2-G OS=Oryza sativa subsp. japonica OX=39947 G... [more]
A2Z9W71.2e-12664.18Putative magnesium transporter MRS2-G OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
A2XCA03.3e-9251.63Putative magnesium transporter MRS2-H OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q10S251.4e-9051.13Putative magnesium transporter MRS2-H OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
A0A6J1C5809.3e-21599.74Magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111008374 PE=3 SV=1[more]
A0A6J1J0737.4e-19691.65Magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111480067 PE=3 SV=1[more]
A0A6J1FRL49.6e-19691.65Magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111447807 PE=3 SV=1[more]
A0A1S3C2A51.2e-19089.82Magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103496094 PE=3 SV=1[more]
A0A5D3CIR61.2e-19089.82Magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold22... [more]
Match NameE-valueIdentityDescription
AT3G58970.14.6e-14268.78magnesium transporter 6 [more]
AT4G28580.11.7e-9149.49magnesium transport 5 [more]
AT5G64560.11.0e-8847.72magnesium transporter 9 [more]
AT5G09690.12.1e-8144.64magnesium transporter 7 [more]
AT5G09690.22.1e-8144.64magnesium transporter 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 191..225
NoneNo IPR availableGENE3D1.20.58.340Magnesium transport protein CorA, transmembrane regioncoord: 84..324
e-value: 9.0E-58
score: 196.6
NoneNo IPR availableGENE3D2.40.128.330coord: 2..82
e-value: 2.6E-15
score: 57.6
NoneNo IPR availablePANTHERPTHR13890:SF2MAGNESIUM TRANSPORTER MRS2-4-RELATEDcoord: 1..392
NoneNo IPR availableSUPERFAMILY144083Magnesium transport protein CorA, transmembrane regioncoord: 329..380
IPR002523Mg2+ transporter protein, CorA-like/Zinc transport protein ZntBPFAMPF01544CorAcoord: 199..386
e-value: 2.2E-6
score: 27.3
IPR039204Magnesium transporter MRS2-likePANTHERPTHR13890RNA SPLICING PROTEIN MRS2, MITOCHONDRIALcoord: 1..392
IPR039204Magnesium transporter MRS2-likeCDDcd12823Mrs2_Mfm1p-likecoord: 11..390
e-value: 1.34767E-113
score: 332.672

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012520.1MS012520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0046873 metal ion transmembrane transporter activity