MS012509 (gene) Bitter gourd (TR) v1

BLAST of MS012509 vs. NCBI nr
Match: TYK22554.1 (uncharacterized protein E5676_scaffold584G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 516.9 bits (1330), Expect = 1.3e-142
Identity = 276/364 (75.82%), Postives = 299/364 (82.14%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS +LDDLD AFL+SDFMSLHFL KVLSLL TLHSQL+ LGQRLHLP G
Sbjct: 14  TATSLHGFYHFLSHELDDLDHAFLSSDFMSLHFLQKVLSLLRTLHSQLIQLGQRLHLPVG 73

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+ASQ+LKSGISRME++H +ASAIASSLQDPHFLR+NPRASRR   
Sbjct: 74  GKWLDEYMDESSRLWEASQVLKSGISRMEVFHVEASAIASSLQDPHFLRFNPRASRR--- 133

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN+FGLEEEN+SLMNTRI PLSLLC NG 
Sbjct: 134 ----------------------VLRAITDFERNVFGLEEENRSLMNTRIPPLSLLCFNGG 193

Query: 181 -------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYG 240
                  GSTSKLNAFNGFRGVLHAVK ISSLLLMILLC LVYCWPESSFHG+ GI+   
Sbjct: 194 SSMSTGMGSTSKLNAFNGFRGVLHAVKNISSLLLMILLCGLVYCWPESSFHGSNGIEN-E 253

Query: 241 EDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERS 300
           ED +Q T FSS FVASM RL+QRVANEI+RVD QPVGILLFEFREAKAAM+GLKVELE+ 
Sbjct: 254 EDQHQRTMFSSSFVASMERLKQRVANEIERVDVQPVGILLFEFREAKAAMEGLKVELEKG 313

Query: 301 LMEGGEEEEEE---VEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMC 354
           L E  EEEEEE   VEIEEKIERL SWFG LR GVDAI+GQLDDF DEIVEGRKKLLDMC
Sbjct: 314 LEEEEEEEEEEEEKVEIEEKIERLNSWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDMC 351

BLAST of MS012509 vs. NCBI nr
Match: XP_008456067.1 (PREDICTED: uncharacterized protein LOC103496110 [Cucumis melo])

HSP 1 Score: 516.9 bits (1330), Expect = 1.3e-142
Identity = 276/365 (75.62%), Postives = 299/365 (81.92%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS +LDDLD AFL+SDFMSLHFL KVLSLL TLHSQL+ LGQRLHLP G
Sbjct: 56  TATSLHGFYHFLSHELDDLDHAFLSSDFMSLHFLQKVLSLLRTLHSQLIQLGQRLHLPVG 115

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+ASQ+LKSGISRME++H +ASAIASSLQDPHFLR+NPRASRR   
Sbjct: 116 GKWLDEYMDESSRLWEASQVLKSGISRMEVFHVEASAIASSLQDPHFLRFNPRASRR--- 175

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN+FGLEEEN+SLMNTRI PLSLLC NG 
Sbjct: 176 ----------------------VLRAITDFERNVFGLEEENRSLMNTRIPPLSLLCFNGG 235

Query: 181 -------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYG 240
                  GSTSKLNAFNGFRGVLHAVK ISSLLLMILLC LVYCWPESSFHG+ GI+   
Sbjct: 236 SSMSTGMGSTSKLNAFNGFRGVLHAVKNISSLLLMILLCGLVYCWPESSFHGSNGIEN-E 295

Query: 241 EDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERS 300
           ED +Q T FSS FVASM RL+QRVANEI+RVD QPVGILLFEFREAKAAM+GLKVELE+ 
Sbjct: 296 EDQHQRTMFSSSFVASMERLKQRVANEIERVDVQPVGILLFEFREAKAAMEGLKVELEKG 355

Query: 301 LMEGGEEEEEE----VEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDM 354
           L E  EEEEEE    VEIEEKIERL SWFG LR GVDAI+GQLDDF DEIVEGRKKLLDM
Sbjct: 356 LEEDEEEEEEEEEEKVEIEEKIERLNSWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDM 394

BLAST of MS012509 vs. NCBI nr
Match: XP_004146285.1 (uncharacterized protein LOC101211523 [Cucumis sativus] >KGN57591.1 hypothetical protein Csa_009796 [Cucumis sativus])

HSP 1 Score: 509.6 bits (1311), Expect = 2.2e-140
Identity = 270/361 (74.79%), Postives = 296/361 (81.99%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS +LDDLD AF++SDFMSLHFL KVLSLL TLHSQL+ LGQRLHLP G
Sbjct: 8   TATSLHGFYHFLSHELDDLDHAFVSSDFMSLHFLQKVLSLLRTLHSQLIQLGQRLHLPVG 67

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+ASQ+LKSGISRME++H +ASAIASSLQDPHFLR+NPRASRR   
Sbjct: 68  GKWLDEYMDESSRLWEASQVLKSGISRMEVFHVEASAIASSLQDPHFLRFNPRASRR--- 127

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN+FGLEEEN+SLMNTRI PLSLLC NG 
Sbjct: 128 ----------------------VLRAITDFERNVFGLEEENRSLMNTRIPPLSLLCFNGS 187

Query: 181 -------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYG 240
                  GSTSKLNAFNGFRGVLHAVK ISSLLLMILLC LVYCWPES FHG+ GI    
Sbjct: 188 SSVSSGMGSTSKLNAFNGFRGVLHAVKNISSLLLMILLCGLVYCWPESIFHGSNGIGN-E 247

Query: 241 EDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERS 300
           ED +Q T FSS F+ASM RL+QRVANEI+RVD QPVGILLFEFREAKAAM+GLKVELE+ 
Sbjct: 248 EDQHQRTMFSSSFIASMERLKQRVANEIERVDVQPVGILLFEFREAKAAMEGLKVELEKG 307

Query: 301 LMEGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMCTHS 354
           L    E++EEEVEIEEKIERL SWFG LR GVDAI+GQLDDF DEIVEGRKKLLDMCTH+
Sbjct: 308 L---EEDDEEEVEIEEKIERLNSWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDMCTHN 339

BLAST of MS012509 vs. NCBI nr
Match: XP_038878659.1 (uncharacterized protein LOC120070841 [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 5.9e-138
Identity = 263/362 (72.65%), Postives = 292/362 (80.66%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SL+GFY FLS +LDDL  AF++SDFMSLHFL KVLSLL TLHS L+ LGQRLHLP G
Sbjct: 7   TATSLNGFYQFLSHELDDLHHAFVSSDFMSLHFLQKVLSLLRTLHSHLIQLGQRLHLPVG 66

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+A Q+LKS ISRMEL+H +ASAIASSLQDPHF+R+NPRASRR   
Sbjct: 67  GKWLDEYMDESSRLWEACQVLKSAISRMELFHVEASAIASSLQDPHFIRFNPRASRR--- 126

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN++GLEEEN+SLMNTRIQPLSLLC NG 
Sbjct: 127 ----------------------VLRAITDFERNVYGLEEENRSLMNTRIQPLSLLCFNGS 186

Query: 181 --------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQY 240
                   GSTSKLNAFNGFRGVLHAVK+ISSLLLMILLCSLVYCWPESSFHG+ G +  
Sbjct: 187 SDGASTGMGSTSKLNAFNGFRGVLHAVKSISSLLLMILLCSLVYCWPESSFHGSNGTENE 246

Query: 241 GEDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELER 300
            +  +Q T FSS FVASM RL+QRVANEIDRVDGQPVGILLFEFREAKAAM+GLKVELE+
Sbjct: 247 NDQHHQRTMFSSSFVASMERLKQRVANEIDRVDGQPVGILLFEFREAKAAMEGLKVELEK 306

Query: 301 SLMEGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMCTH 354
            L     EE+EEVE EEK+ERL  WFG LR GVDAI+GQLDDF DEIVEGRKKLLDMCTH
Sbjct: 307 GL-----EEDEEVETEEKVERLNGWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDMCTH 338

BLAST of MS012509 vs. NCBI nr
Match: KAG6600364.1 (hypothetical protein SDJN03_05597, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 490.3 bits (1261), Expect = 1.4e-134
Identity = 265/360 (73.61%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS QLDDLD AFL+SDFMSLHFLHKVLSLL  LHS L+ LG RLHLP G
Sbjct: 7   TATSLHGFYHFLSHQLDDLDHAFLSSDFMSLHFLHKVLSLLRALHSHLIQLGHRLHLPVG 66

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMD+SSRLWDA Q+LKSGISR+ELYH++AS IASSLQDPH LR+N RAS+R   
Sbjct: 67  GKWLDEYMDDSSRLWDACQVLKSGISRIELYHSEASVIASSLQDPHLLRFNHRASQR--- 126

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI D ERN F LEEEN+ LMNTRIQPLSLLC N  
Sbjct: 127 ----------------------VLRAIYDLERNGFVLEEENRILMNTRIQPLSLLCFNDN 186

Query: 181 ------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYGE 240
                 GSTSK NAFNGFRGVLHAVK+ISSLLLMILLCSLVYCWPESSFH N GI+   +
Sbjct: 187 SALTGMGSTSKSNAFNGFRGVLHAVKSISSLLLMILLCSLVYCWPESSFHANDGIEN-ED 246

Query: 241 DDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERSL 300
           D +Q T FSS FVASMARLRQRVANEIDRV+GQPVGILLFEFREAKAAMD LK ELE++L
Sbjct: 247 DHHQRTMFSSSFVASMARLRQRVANEIDRVEGQPVGILLFEFREAKAAMDDLKTELEKAL 306

Query: 301 MEGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMCTHSR 354
            E  EEEEEE EIEEK E+LKSW G LR+GVDAIVG+LDDF DEIVEGRKKLLD+CTH+R
Sbjct: 307 EE--EEEEEEDEIEEKAEKLKSWNGALRSGVDAIVGELDDFFDEIVEGRKKLLDICTHNR 338

BLAST of MS012509 vs. ExPASy TrEMBL
Match: A0A5D3DG23 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold584G00090 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.5e-143
Identity = 276/364 (75.82%), Postives = 299/364 (82.14%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS +LDDLD AFL+SDFMSLHFL KVLSLL TLHSQL+ LGQRLHLP G
Sbjct: 14  TATSLHGFYHFLSHELDDLDHAFLSSDFMSLHFLQKVLSLLRTLHSQLIQLGQRLHLPVG 73

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+ASQ+LKSGISRME++H +ASAIASSLQDPHFLR+NPRASRR   
Sbjct: 74  GKWLDEYMDESSRLWEASQVLKSGISRMEVFHVEASAIASSLQDPHFLRFNPRASRR--- 133

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN+FGLEEEN+SLMNTRI PLSLLC NG 
Sbjct: 134 ----------------------VLRAITDFERNVFGLEEENRSLMNTRIPPLSLLCFNGG 193

Query: 181 -------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYG 240
                  GSTSKLNAFNGFRGVLHAVK ISSLLLMILLC LVYCWPESSFHG+ GI+   
Sbjct: 194 SSMSTGMGSTSKLNAFNGFRGVLHAVKNISSLLLMILLCGLVYCWPESSFHGSNGIEN-E 253

Query: 241 EDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERS 300
           ED +Q T FSS FVASM RL+QRVANEI+RVD QPVGILLFEFREAKAAM+GLKVELE+ 
Sbjct: 254 EDQHQRTMFSSSFVASMERLKQRVANEIERVDVQPVGILLFEFREAKAAMEGLKVELEKG 313

Query: 301 LMEGGEEEEEE---VEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMC 354
           L E  EEEEEE   VEIEEKIERL SWFG LR GVDAI+GQLDDF DEIVEGRKKLLDMC
Sbjct: 314 LEEEEEEEEEEEEKVEIEEKIERLNSWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDMC 351

BLAST of MS012509 vs. ExPASy TrEMBL
Match: A0A1S3C325 (uncharacterized protein LOC103496110 OS=Cucumis melo OX=3656 GN=LOC103496110 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 6.5e-143
Identity = 276/365 (75.62%), Postives = 299/365 (81.92%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS +LDDLD AFL+SDFMSLHFL KVLSLL TLHSQL+ LGQRLHLP G
Sbjct: 56  TATSLHGFYHFLSHELDDLDHAFLSSDFMSLHFLQKVLSLLRTLHSQLIQLGQRLHLPVG 115

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+ASQ+LKSGISRME++H +ASAIASSLQDPHFLR+NPRASRR   
Sbjct: 116 GKWLDEYMDESSRLWEASQVLKSGISRMEVFHVEASAIASSLQDPHFLRFNPRASRR--- 175

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN+FGLEEEN+SLMNTRI PLSLLC NG 
Sbjct: 176 ----------------------VLRAITDFERNVFGLEEENRSLMNTRIPPLSLLCFNGG 235

Query: 181 -------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYG 240
                  GSTSKLNAFNGFRGVLHAVK ISSLLLMILLC LVYCWPESSFHG+ GI+   
Sbjct: 236 SSMSTGMGSTSKLNAFNGFRGVLHAVKNISSLLLMILLCGLVYCWPESSFHGSNGIEN-E 295

Query: 241 EDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERS 300
           ED +Q T FSS FVASM RL+QRVANEI+RVD QPVGILLFEFREAKAAM+GLKVELE+ 
Sbjct: 296 EDQHQRTMFSSSFVASMERLKQRVANEIERVDVQPVGILLFEFREAKAAMEGLKVELEKG 355

Query: 301 LMEGGEEEEEE----VEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDM 354
           L E  EEEEEE    VEIEEKIERL SWFG LR GVDAI+GQLDDF DEIVEGRKKLLDM
Sbjct: 356 LEEDEEEEEEEEEEKVEIEEKIERLNSWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDM 394

BLAST of MS012509 vs. ExPASy TrEMBL
Match: A0A0A0LC30 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G221740 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 1.0e-140
Identity = 270/361 (74.79%), Postives = 296/361 (81.99%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS +LDDLD AF++SDFMSLHFL KVLSLL TLHSQL+ LGQRLHLP G
Sbjct: 8   TATSLHGFYHFLSHELDDLDHAFVSSDFMSLHFLQKVLSLLRTLHSQLIQLGQRLHLPVG 67

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMDESSRLW+ASQ+LKSGISRME++H +ASAIASSLQDPHFLR+NPRASRR   
Sbjct: 68  GKWLDEYMDESSRLWEASQVLKSGISRMEVFHVEASAIASSLQDPHFLRFNPRASRR--- 127

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI+DFERN+FGLEEEN+SLMNTRI PLSLLC NG 
Sbjct: 128 ----------------------VLRAITDFERNVFGLEEENRSLMNTRIPPLSLLCFNGS 187

Query: 181 -------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYG 240
                  GSTSKLNAFNGFRGVLHAVK ISSLLLMILLC LVYCWPES FHG+ GI    
Sbjct: 188 SSVSSGMGSTSKLNAFNGFRGVLHAVKNISSLLLMILLCGLVYCWPESIFHGSNGIGN-E 247

Query: 241 EDDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERS 300
           ED +Q T FSS F+ASM RL+QRVANEI+RVD QPVGILLFEFREAKAAM+GLKVELE+ 
Sbjct: 248 EDQHQRTMFSSSFIASMERLKQRVANEIERVDVQPVGILLFEFREAKAAMEGLKVELEKG 307

Query: 301 LMEGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMCTHS 354
           L    E++EEEVEIEEKIERL SWFG LR GVDAI+GQLDDF DEIVEGRKKLLDMCTH+
Sbjct: 308 L---EEDDEEEVEIEEKIERLNSWFGSLRIGVDAIIGQLDDFFDEIVEGRKKLLDMCTHN 339

BLAST of MS012509 vs. ExPASy TrEMBL
Match: A0A6J1J2L7 (uncharacterized protein LOC111480769 OS=Cucurbita maxima OX=3661 GN=LOC111480769 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.5e-134
Identity = 265/369 (71.82%), Postives = 291/369 (78.86%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS QLDDLD AFL+SDFMSLHFLHKVLSLL  LH+ L+ LG RLHLP G
Sbjct: 7   TATSLHGFYHFLSHQLDDLDHAFLSSDFMSLHFLHKVLSLLRALHTHLIQLGHRLHLPVG 66

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMD+SSRLWDA Q+LKSGISR+ELYH++ASAI+SSLQDPH LR+N RAS+R   
Sbjct: 67  GKWLDEYMDDSSRLWDACQVLKSGISRIELYHSEASAISSSLQDPHLLRFNHRASQR--- 126

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI D ERN F LEEEN+ LMNTRIQPLSLLC N  
Sbjct: 127 ----------------------VLRAIYDLERNGFVLEEENRILMNTRIQPLSLLCFNDN 186

Query: 181 ------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYGE 240
                 GSTSK NAFNGFRGVLHAVK+ISSLLLMILLCSLVYCWPESSFH N GI+   +
Sbjct: 187 SALTGMGSTSKSNAFNGFRGVLHAVKSISSLLLMILLCSLVYCWPESSFHANDGIEN-ED 246

Query: 241 DDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERSL 300
           D +Q T FSS FVASMARLRQRVANEIDRV+GQPVGILLFEFREAKAAMD LK ELE+ L
Sbjct: 247 DHHQRTTFSSSFVASMARLRQRVANEIDRVEGQPVGILLFEFREAKAAMDDLKTELEKGL 306

Query: 301 ---------MEGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKK 354
                     E  EEEEEEVEIEEK+E+LKSW G LR+GVDAIVG+LDDF DEIVEGRKK
Sbjct: 307 EEEEEEEEEEEEEEEEEEEVEIEEKLEKLKSWNGALRSGVDAIVGELDDFFDEIVEGRKK 349

BLAST of MS012509 vs. ExPASy TrEMBL
Match: A0A6J1FMG3 (uncharacterized protein LOC111447146 OS=Cucurbita moschata OX=3662 GN=LOC111447146 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 4.2e-134
Identity = 264/360 (73.33%), Postives = 289/360 (80.28%), Query Frame = 0

Query: 1   TSSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPG 60
           T++SLHGFYHFLS QLDDLD AFL+SDFMSLHFLHKVLSLL  LHS L+ LG RLHLP G
Sbjct: 7   TATSLHGFYHFLSHQLDDLDHAFLSSDFMSLHFLHKVLSLLRALHSHLIQLGHRLHLPVG 66

Query: 61  GKWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRF 120
           GKWLDEYMD+SSRLWDA Q+LKSGISR+ELYH++ASAIASSLQDPH LR+N RAS+R   
Sbjct: 67  GKWLDEYMDDSSRLWDACQVLKSGISRIELYHSEASAIASSLQDPHLLRFNHRASQR--- 126

Query: 121 VFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSLLCSNG- 180
                                 VLRAI D ERN F LEEEN+ LMNTRIQPLSLLC N  
Sbjct: 127 ----------------------VLRAIYDLERNGFVLEEENRILMNTRIQPLSLLCFNDN 186

Query: 181 ------GSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYGE 240
                 GSTSK NAFNGFRGVLHAVK+ISSLLLMILLCSLVYCWPESSFH N GI+   +
Sbjct: 187 SALTGMGSTSKSNAFNGFRGVLHAVKSISSLLLMILLCSLVYCWPESSFHANDGIEN-ED 246

Query: 241 DDNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERSL 300
           D +Q T FSS FVASMARLRQRVANEIDRV+GQPVGILLFEFREAKAAMD LK ELE++L
Sbjct: 247 DHHQRTMFSSSFVASMARLRQRVANEIDRVEGQPVGILLFEFREAKAAMDDLKTELEKAL 306

Query: 301 MEGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMCTHSR 354
               EEEEEE EIEEK E+LKSW G LR+GVDAIVG+LDDF DEIVEGRKKLLD+CT++R
Sbjct: 307 ---EEEEEEEDEIEEKAEKLKSWNGALRSGVDAIVGELDDFFDEIVEGRKKLLDICTYNR 337

BLAST of MS012509 vs. TAIR 10
Match: AT1G22030.1 (CONTAINS InterPro DOMAIN/s: Protein BYPASS related (InterPro:IPR008511); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G77855.1); Has 99 Blast hits to 99 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 0; Plants - 96; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 272.3 bits (695), Expect = 5.4e-73
Identity = 162/363 (44.63%), Postives = 224/363 (61.71%), Query Frame = 0

Query: 2   SSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPGG 61
           ++S++GFY FL++ ++DL+  +L+++FMS+HFL + L LL T HS L LL Q+L LP G 
Sbjct: 6   ANSVNGFYSFLNRSMEDLERVYLSNNFMSVHFLQRALCLLRTSHSHLTLLVQKLQLPVGD 65

Query: 62  KWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLRYNPRASRRVRFV 121
           KWLDEYMDESS+LW+A  ++KS +S +E + +   +IAS+L      R +P+ SR     
Sbjct: 66  KWLDEYMDESSKLWEACLVIKSAVSSVENFSSAGISIASTLD----RRLSPQLSR----- 125

Query: 122 FNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPL------SLL 181
                               QV+RAIS   R   G+EEEN++LM  R+Q        +  
Sbjct: 126 --------------------QVIRAISGCRREAIGIEEENRALMENRVQRFPFWSEQTSA 185

Query: 182 CSNGGSTSKLNAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYGEDD 241
            +   ST   N F+GFRGVL+A + +SSLLLM+L+  LVYC+P     G+A   Q     
Sbjct: 186 TAMESSTKLQNGFSGFRGVLYATRNMSSLLLMVLMNGLVYCFP-----GDAA-TQTQTQI 245

Query: 242 NQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERSLME 301
            Q  +   GF  +M RL+QRVA E+ R+ G   GIL+ E+R +KAA++ LK ELER    
Sbjct: 246 TQTQSQVGGFAGAMGRLQQRVAAEVGRM-GIRKGILMHEYRRSKAALEELKAELERRFCG 305

Query: 302 GG-------EEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDM 352
           GG       EEEE+E E+ E++E LK +FG LRNG ++IV Q+DDF DEIVEGRKKLLD 
Sbjct: 306 GGGGGGEREEEEEDERELRERVENLKGYFGNLRNGTESIVAQIDDFFDEIVEGRKKLLDF 332

BLAST of MS012509 vs. TAIR 10
Match: AT1G77855.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G22030.1); Has 120 Blast hits to 120 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 120; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 244.2 bits (622), Expect = 1.6e-64
Identity = 151/358 (42.18%), Postives = 217/358 (60.61%), Query Frame = 0

Query: 2   SSSLHGFYHFLSQQLDDLDAAFLASDFMSLHFLHKVLSLLTTLHSQLLLLGQRLHLPPGG 61
           S+S++GFY FL++ ++DL+  +++++FMSL FL +V+ LL T HS L LL Q+L+LP G 
Sbjct: 6   SNSVNGFYSFLNRSMEDLERVYISNNFMSLQFLQRVICLLRTSHSHLTLLVQKLNLPVGD 65

Query: 62  KWLDEYMDESSRLWDASQLLKSGISRMELYHTQASAIASSLQDPHFLR--YNPRASRRVR 121
           KWLD+YMDE+S+LWD   ++KS IS +E + + A +I S+L   +  R   +P+ SR   
Sbjct: 66  KWLDDYMDETSKLWDVCHVIKSAISTIESFCSSAISITSTLDGHYHHRRLLSPQISR--- 125

Query: 122 FVFNFVSSVFSSVFLCFNFWVFQVLRAISDFERNIFGLEEENKSLMNTRIQPLSL----L 181
                                 QV+RAIS   R   G+EEEN++LM  RIQ        +
Sbjct: 126 ----------------------QVIRAISGCRREAVGIEEENRALMENRIQRFPFWSEQV 185

Query: 182 CSNGGSTSKL-NAFNGFRGVLHAVKTISSLLLMILLCSLVYCWPESSFHGNAGIDQYGED 241
            + G  +SK+ N F+GFRGV++ +K I+SLLL+IL+  LVY  P                
Sbjct: 186 TTTGMESSKIQNGFSGFRGVMNTMKNINSLLLVILMQGLVYYIPGD-------------- 245

Query: 242 DNQMTAFSSGFVASMARLRQRVANEIDRVDGQPVGILLFEFREAKAAMDGLKVELERSLM 301
               T   +G   +M RL+QRVA E++R+ G   G++++E+R +K AM+ LKVELER   
Sbjct: 246 ----TTVPTG---TMMRLKQRVAAEMERI-GVRKGMMMYEYRRSKTAMEELKVELERRCC 305

Query: 302 -EGGEEEEEEVEIEEKIERLKSWFGMLRNGVDAIVGQLDDFLDEIVEGRKKLLDMCTH 352
             GGEEE  E  + E+IE LK   G LRNG ++IV Q+DDF D+IV+GRK LLD C+H
Sbjct: 306 GGGGEEEAVEKGLRERIENLKGSVGSLRNGTESIVAQIDDFFDDIVDGRKMLLDYCSH 316