MS012501 (gene) Bitter gourd (TR) v1

Overview


Name	MS012501
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	glutelin type-A 2-like
Location	scaffold63: 1128435 .. 1130259 (+)
RNA-Seq Expression	MS012501
Synteny	MS012501

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGCAGCTGAACTTGCAGCCTATGAGTCCCAAGCCCTTCTTTGAAGGGGACGCCGGATCCTATCAAAAATGGCTGCCCTCCGACTATCCATTGCTTGCTCAGACCAAGGTTGCCGCCGGCAGGCTTCTCCTCCGCCCTGGCGGTTTCGTCGTTCCTCACTACGCTGATTCCTCCAAGGTGGGCTACGTACTTCGCGGTAAATTCCTTCCATTTCTAAAATTTAATTTCATATCCTTTTTCCTTCTTCGCCCTCTCTCTAATTACGAGGATGTTTGCAGGTGGCGACGGAGTTGCCGGATTTGTGTTTCCGAACAAGTCCGACGAAGTGGTGGTGAAGTTAAGAGAAGGAGATTTGATCCCAGTGCCAGCAGGAGTCACGTCGTGGTGGTTCAACGATGGAGACTCCGATTTTGAGATAGTCTTTTTGGGTGAAACCAAAAGTGCGCATGTGCCTGGGGACTTTACTTACTTCATTCTCTCCGGCTCCCTTGGCCTCCTGCAAGGCTTTTCGCCGGAGTATGTCGGGAAAACCTACAACCTCAACGGAGAAGAAACGACCACGCTTCTCAAAAGCCAATCCAACGGCTTGATCTTCTCCCTTCAACCATCCCAATCTCTCCCTAAACCCCACAAACGCAGCAAGCTAGTTTACAACATTGACGCCGCCGCGCCGGACGGCAGAACCAAAGCCGGCGGCGGGTCCGTCACGACGGTGACGGAATCCAAATTTCCGTTCATTGGCCAATCTGGGCTGACGGCGGTTCTCGAGAAGATCGAGGCCAACGCCGTCCGGTCGCCGGTGTACGTAGCGGAGGCGGCGGATCAGCTGATCTACGTGGCGGAAGGATCCGGGAGAATTCAGATCGTGGGATTTGCGAGTAAAATTGATGCAGAGGTGAAATCAGGTCAACTGCTTTTGGTGCCGAAATATTTCGCCGTCGGAAAGATCGCCGGAGAAGAAGGCTTGGAGTGCATCTCCGTTATCACAGCTACACAGTAAGCTTGTTTATTTATTTATTTATTTTTGGGTTAAATTACAATTTTCAATCATTTTCTTTTTTTAAAATTCTTAGACAAAAACTTCTTACAATTTTATTTACGTAGCCCATATATCTAAATATATTTTTAAAAATTTTATTAGATGCTTCAACTTTTTAAATTGTGATTGTTTCAACTTGGATAATGATTTGGTTTTTAATTTTGTATTTTTTTAAATGATACTTACTCATTCATAATTTCTTTATATGTCTTTCACATTTTTTGGAAGTATATTTTAATTTTAAGCCAAATTAAAAAATAGACATATTTTTTTCTAAATTACTTTTTCTAGTTTCAAAAATTAATAATATTTAGAAATGTTTACTAATAAATAAGTATCTCTTATTTACCATTATATTAAAAGATTGACAATTATTTATTATATTTTCTCTCTTTAGTTATCTAAGATAGTCCGTCATTTTTTTAATAATAGTAAGTAATGGGATAACTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTATAAACTTAAATTTTTAAAAAATATAAAACAAAGAAAACAAAATAATTATATAATTATATATATATATATATATATATTTTATTTTTTTCTGAATCTTTCAACTTTGGAACATATTTTGATGTGATGTGAAATGAATGAATTAACAGCCCTGTAGTGGAAGATTTGGCCGGAAAAACGTGCGTTTACGAGGCGCTGTCGCCGGAGGTTGTTCAAGTTTCGTTCAACGTCACGGCGGAGTTCGAAAAGCTTCTCAGGTCCAAAATAACCAAAGCTTCGCCTCTGGTTCCTCCTTCCGATAAT

mRNA sequence

GAGCAGCTGAACTTGCAGCCTATGAGTCCCAAGCCCTTCTTTGAAGGGGACGCCGGATCCTATCAAAAATGGCTGCCCTCCGACTATCCATTGCTTGCTCAGACCAAGGTTGCCGCCGGCAGGCTTCTCCTCCGCCCTGGCGGTTTCGTCGTTCCTCACTACGCTGATTCCTCCAAGGTGGGCTACGTACTTCGCGGTGGCGACGGAGTTGCCGGATTTGTGTTTCCGAACAAGTCCGACGAAGTGGTGGTGAAGTTAAGAGAAGGAGATTTGATCCCAGTGCCAGCAGGAGTCACGTCGTGGTGGTTCAACGATGGAGACTCCGATTTTGAGATAGTCTTTTTGGGTGAAACCAAAAGTGCGCATGTGCCTGGGGACTTTACTTACTTCATTCTCTCCGGCTCCCTTGGCCTCCTGCAAGGCTTTTCGCCGGAGTATGTCGGGAAAACCTACAACCTCAACGGAGAAGAAACGACCACGCTTCTCAAAAGCCAATCCAACGGCTTGATCTTCTCCCTTCAACCATCCCAATCTCTCCCTAAACCCCACAAACGCAGCAAGCTAGTTTACAACATTGACGCCGCCGCGCCGGACGGCAGAACCAAAGCCGGCGGCGGGTCCGTCACGACGGTGACGGAATCCAAATTTCCGTTCATTGGCCAATCTGGGCTGACGGCGGTTCTCGAGAAGATCGAGGCCAACGCCGTCCGGTCGCCGGTGTACGTAGCGGAGGCGGCGGATCAGCTGATCTACGTGGCGGAAGGATCCGGGAGAATTCAGATCGTGGGATTTGCGAGTAAAATTGATGCAGAGGTGAAATCAGGTCAACTGCTTTTGGTGCCGAAATATTTCGCCGTCGGAAAGATCGCCGGAGAAGAAGGCTTGGAGTGCATCTCCGTTATCACAGCTACACACCCTGTAGTGGAAGATTTGGCCGGAAAAACGTGCGTTTACGAGGCGCTGTCGCCGGAGGTTGTTCAAGTTTCGTTCAACGTCACGGCGGAGTTCGAAAAGCTTCTCAGGTCCAAAATAACCAAAGCTTCGCCTCTGGTTCCTCCTTCCGATAAT

Coding sequence (CDS)

GAGCAGCTGAACTTGCAGCCTATGAGTCCCAAGCCCTTCTTTGAAGGGGACGCCGGATCCTATCAAAAATGGCTGCCCTCCGACTATCCATTGCTTGCTCAGACCAAGGTTGCCGCCGGCAGGCTTCTCCTCCGCCCTGGCGGTTTCGTCGTTCCTCACTACGCTGATTCCTCCAAGGTGGGCTACGTACTTCGCGGTGGCGACGGAGTTGCCGGATTTGTGTTTCCGAACAAGTCCGACGAAGTGGTGGTGAAGTTAAGAGAAGGAGATTTGATCCCAGTGCCAGCAGGAGTCACGTCGTGGTGGTTCAACGATGGAGACTCCGATTTTGAGATAGTCTTTTTGGGTGAAACCAAAAGTGCGCATGTGCCTGGGGACTTTACTTACTTCATTCTCTCCGGCTCCCTTGGCCTCCTGCAAGGCTTTTCGCCGGAGTATGTCGGGAAAACCTACAACCTCAACGGAGAAGAAACGACCACGCTTCTCAAAAGCCAATCCAACGGCTTGATCTTCTCCCTTCAACCATCCCAATCTCTCCCTAAACCCCACAAACGCAGCAAGCTAGTTTACAACATTGACGCCGCCGCGCCGGACGGCAGAACCAAAGCCGGCGGCGGGTCCGTCACGACGGTGACGGAATCCAAATTTCCGTTCATTGGCCAATCTGGGCTGACGGCGGTTCTCGAGAAGATCGAGGCCAACGCCGTCCGGTCGCCGGTGTACGTAGCGGAGGCGGCGGATCAGCTGATCTACGTGGCGGAAGGATCCGGGAGAATTCAGATCGTGGGATTTGCGAGTAAAATTGATGCAGAGGTGAAATCAGGTCAACTGCTTTTGGTGCCGAAATATTTCGCCGTCGGAAAGATCGCCGGAGAAGAAGGCTTGGAGTGCATCTCCGTTATCACAGCTACACACCCTGTAGTGGAAGATTTGGCCGGAAAAACGTGCGTTTACGAGGCGCTGTCGCCGGAGGTTGTTCAAGTTTCGTTCAACGTCACGGCGGAGTTCGAAAAGCTTCTCAGGTCCAAAATAACCAAAGCTTCGCCTCTGGTTCCTCCTTCCGATAAT

Protein sequence

EQLNLQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLRGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPGDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKRSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEAADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATHPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSDN

Homology

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BLAST of MS012501 vs. NCBI nr
Match: XP_022922755.1 (legumin J-like [Cucurbita moschata])

HSP 1 Score: 570.5 bits (1469), Expect = 1.0e-158
Identity = 282/350 (80.57%), Postives = 313/350 (89.43%), Query Frame = 0


Query: 6   QPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLR 65
           QPM+PKPF E +AGSY KWLPS+YPLLAQ KVAAGRLLLRP GFVVPHYAD SKVGYVL+
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 66  GGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPG 125
           G +GVAG VFP+KSDEVVV L++GDLIPVP GV+SWWFNDGDSD EI+FLGE+K+AHVPG
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 126 DFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKR 185
           D +YF+LSG L LL GFSPEYVGKTY+LNGEETT  LKSQSN LIFS+Q +QSLPKP K 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKY 182

Query: 186 SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEA 245
           SK VYNIDAAAPDGR K G G+VTTVTESKFPFIGQSGLTA+LEK+ ANAVRSPVYVAE 
Sbjct: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVAEP 242

Query: 246 ADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATH 305
            DQLIYVA+G G+IQIVG +SKIDAEVK GQL+LVPK+FAVGKIAGE+GLECIS+ITATH
Sbjct: 243 YDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302

Query: 306 PVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           PVVE+LAGKT V EALSPE+ QVSFNVTAEFEKLLRSKIT ASP++  SD
Sbjct: 303 PVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

[Close]

BLAST of MS012501 vs. NCBI nr
Match: XP_022985328.1 (12S seed storage protein CRD-like [Cucurbita maxima])

HSP 1 Score: 570.5 bits (1469), Expect = 1.0e-158
Identity = 282/350 (80.57%), Postives = 314/350 (89.71%), Query Frame = 0


Query: 6   QPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLR 65
           QPM+PKPF E +AGSY KWLPS+YPLLA  KVAAGRLLLRP GFVVPHYAD SKVGYVL+
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 66  GGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPG 125
           G +GVAG VFP+KSDEVVV L++GDLIPVP GV+SWWFNDGDSD EI+FLGE+K+AHVPG
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 126 DFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKR 185
           D +YF+LSG L LL GFSPEYVG+TY+LNGEETT  LKSQSN LIFS+Q +QSLPKP K 
Sbjct: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182

Query: 186 SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEA 245
           SK VYNIDAAAPDGR K G G+VTTVTESKFPFIGQSGLTA+LEK++ANAVRSPVYVAE 
Sbjct: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242

Query: 246 ADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATH 305
            DQLIYVA+G G+IQIVGF+SKIDAEVK GQL+LVPK+FAVGKIAGE+GLECIS+ITATH
Sbjct: 243 YDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302

Query: 306 PVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           PVVE+LAGKT V EALSPEV QVSFNVTAEFEKLLRSKIT ASP++  SD
Sbjct: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 352

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BLAST of MS012501 vs. NCBI nr
Match: KAG6576976.1 (12S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 567.8 bits (1462), Expect = 6.7e-158
Identity = 280/350 (80.00%), Postives = 314/350 (89.71%), Query Frame = 0


Query: 6   QPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLR 65
           QPM+PKPF E +AGSY KWLPS+YPLLA+ KVAAGRLLLRP GFVVPHYAD SKVGYVL+
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 66  GGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPG 125
           G +GVAG VFP+KSDEVVV L++GDLIPVP GV+SWWFN+GDSD EI+FLGE+K+AHVPG
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNEGDSDLEIIFLGESKNAHVPG 122

Query: 126 DFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKR 185
           D +YF+LSG L LL GFSPEYVGKTY+LNGEETT  LKSQSN LIFS+Q +QSLPKP K 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKF 182

Query: 186 SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEA 245
           SK VYNIDAAAPDGR K G G+VTTVTESKFPFIGQSGLTA+LEK++ANAVRSPVYVAE 
Sbjct: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242

Query: 246 ADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATH 305
            DQLIYVA+G G+IQIVG +SKIDAEVK GQL+LVPK+FAVGKIAGE+GLECIS+ITATH
Sbjct: 243 YDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302

Query: 306 PVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           PVVE+LAGKT V EALSPE+ QVSFNVTAEFEKLLRSKIT ASP++  SD
Sbjct: 303 PVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

[Close]

BLAST of MS012501 vs. NCBI nr
Match: XP_023552908.1 (12S seed storage globulin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 560.8 bits (1444), Expect = 8.2e-156
Identity = 278/350 (79.43%), Postives = 310/350 (88.57%), Query Frame = 0


Query: 6   QPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLR 65
           QPM+PKPF E +AGSY KWLPS+YPLLA+ KVAAGRLLLRP GFVVPHYAD SKVGYVL+
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 66  GGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPG 125
           G +GV G VFP+KSDEVVV L++GDLIPVP GV+SWWFNDGDSD EI+FLGE+K+AHVPG
Sbjct: 63  GENGVVGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 126 DFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKR 185
           D +YF+LSG L LL GFSPEYVGKTY+LNGEETT  LKSQSN LI S+Q +QSLPKP K 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALICSIQQTQSLPKPPKF 182

Query: 186 SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEA 245
           SK VYNIDAAAPDGR K   G+VTTVTESKFPFIGQSGLTA+LEK++ANAVRSPVYVAE 
Sbjct: 183 SKFVYNIDAAAPDGRVKGSAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242

Query: 246 ADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATH 305
            DQLIYVA+G G+IQIVG +SKIDAEVK GQL+LVPK+FAVGK AGE+GLECIS+ITATH
Sbjct: 243 YDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKFAGEDGLECISIITATH 302

Query: 306 PVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           PVVE+LAGKT V EALSPEV QVSFNVTAEFEKLLRSKIT ASP++  SD
Sbjct: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

[Close]

BLAST of MS012501 vs. NCBI nr
Match: XP_008456076.1 (PREDICTED: glutelin type-A 2-like [Cucumis melo] >KAA0039043.1 glutelin type-A 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 541.6 bits (1394), Expect = 5.2e-150
Identity = 261/340 (76.76%), Postives = 299/340 (87.94%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           ++ M+PKPFFEG+ GSY KWLPSDYPLLAQT VA GRLLLRP GF VPHYAD SK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 65  RGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVP 124
           +G DGV GFVFPNK +EVV+KL++GDLIPVP+G+TSWWFNDGDSD EI+FLGETK+AHVP
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 125 GDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHK 184
           GD TYFILSG  GLLQGF+PEYV K+Y+L+ EET   LKSQSN LIF++QPSQSLPKPHK
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHK 180

Query: 185 RSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAE 244
            SKLVYNIDAA PD R K G  +VT VTES FPFIGQ+GLTAVLEK++ANA+RSPVY+AE
Sbjct: 181 HSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIAE 240

Query: 245 AADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITAT 304
            +DQLIYV +GSG+IQ+VGF+SK DA+VK GQL+LVP+YFAVGK+AGEEGLECIS+I AT
Sbjct: 241 PSDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVAT 300

Query: 305 HPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKI 345
           HP+VE+LAGKT V EALS EV QVSFNVTAEFEKL RSK+
Sbjct: 301 HPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

[Close]

BLAST of MS012501 vs. ExPASy Swiss-Prot
Match: O23880 (13S globulin seed storage protein 2 OS=Fagopyrum esculentum OX=3617 GN=FA18 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-20
Identity = 103/425 (24.24%), Postives = 169/425 (39.76%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           L    P      +AG  + W   D P    T   A R++++PGG ++P Y+++  + +V 
Sbjct: 51  LTASEPSRRVRSEAGVTEIW-DHDTPEFRCTGFVAVRVVIQPGGLLLPSYSNAPYITFV- 110

Query: 65  RGGDGVAGFVFPN-----KSD-----------------------------EVVVKLREGD 124
             G GV G V P      +SD                             + + ++REGD
Sbjct: 111 EQGRGVQGVVIPGCPETFQSDSEFEYPQSQRGRHSRQSESEEESSRGDQHQKIFRIREGD 170

Query: 125 LIPVPAGVTSWWFNDGDSDFEIVFLGETKSAH--VPGDFTYFILSG-------------- 184
           +IP PAGV  W  NDG+ D   V L +  S H  +  +   F L+G              
Sbjct: 171 VIPSPAGVVQWTHNDGNDDLISVTLLDANSYHKQLDENVRSFFLAGQSQRETREEGSDRQ 230

Query: 185 -----------SLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSN--GLI-----FSLQPS 244
                         +L GF  E + + +     ET + L+ +++  G I       L+  
Sbjct: 231 SRESDDDEALLGANILSGFQDEILHELFRDVDRETISKLRGENDQRGFIVQAQDLKLRVP 290

Query: 245 QSLPKPHKRS------------------------KLVYNIDAAAPDGRTKAGGGSVTTVT 304
           Q   + ++R                         K   N +            G + TV 
Sbjct: 291 QDFEEEYERERGDRRRGQGGSGRSNGVEQGFCNLKFRRNFNTPTNTYVFNPRAGRINTVN 350

Query: 305 ESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEAADQLIYVAEGSGRIQIVGFASK--IDA 336
            +  P +    L+A    +  NA+  P +   A   L YV  G GR+Q+VG   K   D 
Sbjct: 351 SNSLPILEFLQLSAQHVVLYKNAIIGPRWNLNAHSAL-YVTRGEGRVQVVGDEGKSVFDD 410

[Close]

BLAST of MS012501 vs. ExPASy Swiss-Prot
Match: Q02897 (Glutelin type-B 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUB2 PE=2 SV=2)

HSP 1 Score: 100.9 bits (250), Expect = 3.1e-20
Identity = 84/382 (21.99%), Postives = 163/382 (42.67%), Query Frame = 0


Query: 41  RLLLRPGGFVVPHYADSSKVGYVLRGGDGVAGFVFP------------------------ 100
           R +++P G +VP Y+++  + Y+++ G G  G  FP                        
Sbjct: 85  RRVIQPQGLLVPRYSNTPGLVYIIQ-GRGSMGLTFPGCPATYQQQFQQFSSQGQSQSQKF 144

Query: 101 NKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSA--HVPGDFTYFILSG 160
               + + + R+GD++ +PAGV  W++NDGD+    +++ +  ++   +      F+L+G
Sbjct: 145 RDEHQKIHQFRQGDVVALPAGVAHWFYNDGDASVVAIYVYDINNSANQLEPRQKEFLLAG 204

Query: 161 -----------------SLGLLQGFSPEYVGKTYNLNGEETTTL-LKSQSNGLIFSLQPS 220
                            S  +  GF  E + +   +N      L  ++   G I  ++  
Sbjct: 205 NNNRVQQVYGSSIEQHSSQNIFNGFGTELLSEALGINTVAAKRLQSQNDQRGEIVHVKNG 264

Query: 221 QSLPKP-----HKRSKLVY-----------------------------NIDAAAPDGRTK 280
             L KP      ++++  Y                             NI+  +      
Sbjct: 265 LQLLKPTLTQQQEQAQAQYQEVQYSEQQQTSSRWNGLEENFCTIKARVNIENPSRADSYN 324

Query: 281 AGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEAADQLIYVAEGSGRIQIV 340
              G +++V   KFP +    ++A    +  NA+ SP +    A  L+Y+ +G  R+Q+V
Sbjct: 325 PRAGRISSVNSQKFPILNLIQMSATRVNLYQNAILSPFWNVN-AHSLVYMIQGQSRVQVV 384

Query: 341 GFASK--IDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATHPVVEDLAGKTCVYEA 343
               K   D  ++ GQLL++P+++AV K A  EG + I++ T  +  V  LAGK  V+ A
Sbjct: 385 SNFGKTVFDGVLRPGQLLIIPQHYAVLKKAEREGCQYIAIKTNANAFVSHLAGKNSVFRA 444

[Close]

BLAST of MS012501 vs. ExPASy Swiss-Prot
Match: P14323 (Glutelin type-B 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GluB1-A PE=2 SV=3)

HSP 1 Score: 100.5 bits (249), Expect = 4.0e-20
Identity = 87/386 (22.54%), Postives = 157/386 (40.67%), Query Frame = 0


Query: 41  RLLLRPGGFVVPHYADSSKVGYVLRGGDGVAGFVFP------------------------ 100
           R +++P G +VP Y +   V Y+++ G G  G  FP                        
Sbjct: 85  RRVIQPQGLLVPRYTNIPGVVYIIQ-GRGSMGLTFPGCPATYQQQFQQFSSQGQSQSQKF 144

Query: 101 NKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGE--TKSAHVPGDFTYFILSG 160
               + + + R+GD++ +PAGV  W++NDGD+    V++ +    +  +      F+L+G
Sbjct: 145 RDEHQKIHQFRQGDIVALPAGVAHWFYNDGDAPIVAVYVYDVNNNANQLEPRQKEFLLAG 204

Query: 161 S-------------------LGLLQGFSPEYVGKTYNLNGEETTTL-LKSQSNGLIFSLQ 220
           +                     +  GF  E + +   +N      L  ++   G I  ++
Sbjct: 205 NNNRAQQQQVYGSSIEQHSGQNIFSGFGVEMLSEALGINAVAAKRLQSQNDQRGEIIHVK 264

Query: 221 PSQSLPKPHKRS------------------------------------KLVYNIDAAAPD 280
               L KP                                        K+  NI+  +  
Sbjct: 265 NGLQLLKPTLTQQQEQAQAQDQYQQVQYSERQQTSSRWNGLEENFCTIKVRVNIENPSRA 324

Query: 281 GRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEAADQLIYVAEGSGR 340
                  G +T+V   KFP +    ++A    +  NA+ SP +    A  L+Y+ +G  R
Sbjct: 325 DSYNPRAGRITSVNSQKFPILNLIQMSATRVNLYQNAILSPFWNVN-AHSLVYMIQGRSR 384

Query: 341 IQIVGFASK--IDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATHPVVEDLAGKTC 343
           +Q+V    K   D  ++ GQLL++P+++AV K A  EG + I++ T  +  V  LAGK  
Sbjct: 385 VQVVSNFGKTVFDGVLRPGQLLIIPQHYAVLKKAEREGCQYIAIKTNANAFVSHLAGKNS 444

[Close]

BLAST of MS012501 vs. ExPASy Swiss-Prot
Match: Q9ZWA9 (12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.2e-20
Identity = 97/416 (23.32%), Postives = 164/416 (39.42%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           +  ++P    + +AG  + W     P L    V   R+ L+P    +P +     + YV+
Sbjct: 41  INSLAPAQATKFEAGQMEVW-DHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVV 100

Query: 65  -------------------------RGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVT 124
                                    RGG G  G  F +   + +   R GD+    AGV+
Sbjct: 101 QGEGVMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDMHQK-LENFRRGDVFASLAGVS 160

Query: 125 SWWFNDGDSDFEIVFL-----GETKSAHVPGDFTYFILSGSL--------------GLLQ 184
            WW+N GDSD  IV +      E +   VP     F L+GS                   
Sbjct: 161 QWWYNRGDSDAVIVIVLDVTNRENQLDQVP---RMFQLAGSRTQEEEQPLTWPSGNNAFS 220

Query: 185 GFSPEYVGKTYNLNGEETTTLLKSQSN---------GLIFSLQP---------SQSLPKP 244
           GF P  + + + +N E    L   + N          L F + P         +  + + 
Sbjct: 221 GFDPNIIAEAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEET 280

Query: 245 HKRSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYV 304
           +  +K+  NID            G ++T+     P +    L A+   + +  +  P + 
Sbjct: 281 YCTAKIHENIDDPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWT 340

Query: 305 AEAADQLIYVAEGSGRIQIV--GFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISV 357
           A  A  ++YV  G  +IQ+V     S  + +V  GQ++++P+ FAV K AGE G E IS 
Sbjct: 341 AN-AHTVLYVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISF 400

[Close]

BLAST of MS012501 vs. ExPASy Swiss-Prot
Match: Q09151 (Glutelin type-A 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA3 PE=2 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 6.8e-20
Identity = 100/424 (23.58%), Postives = 175/424 (41.27%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           LQ   P       AG+ + +  S+  L   T V   R ++ P G ++PHY++ + + YV+
Sbjct: 50  LQAFEPIRTVRSQAGTTEFFDVSN-ELFQCTGVFVVRRVIEPRGLLLPHYSNGATLVYVI 109

Query: 65  RGGDGVAGFVFP----------------------------NKSDEVVVKLREGDLIPVPA 124
           + G G+ G  FP                                + + + ++GD++ +PA
Sbjct: 110 Q-GRGITGPTFPGCPETYQQQFQQSEQDQQLEGQSQSHKFRDEHQKIHRFQQGDVVALPA 169

Query: 125 GVTSWWFNDGDSDFEIVFL--------------------GETK-------------SAHV 184
           GV  W +NDGD+    +++                    G  K             S +V
Sbjct: 170 GVAHWCYNDGDAPIVAIYVTDIYNSANQLDPRHRDFFLAGNNKIGQQLYRYEARDNSKNV 229

Query: 185 PGDFTYFILSGSLGLLQGFS----------PEYVGKTYNLNGEETTTLLKSQSNGLIFS- 244
            G F+  +LS +LG+  G +           E V   + L+  +    L+ Q    + S 
Sbjct: 230 FGGFSVELLSEALGISSGVARQLQCQNDQRGEIVRVEHGLSLLQPYASLQEQQQEQVQSR 289

Query: 245 -----------LQPSQS--LPKPHKRSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFI 304
                      LQ S S  L +     ++  NID            G +T +   KFP +
Sbjct: 290 DYGQTQYQQKQLQGSCSNGLDETFCTMRVRQNIDNPNLADTYNPRAGRITYLNGQKFPIL 349

Query: 305 GQSGLTAVLEKIEANAVRSPVYVAEAADQLIYVAEGSGRIQIVGFASK--IDAEVKSGQL 342
               ++AV   +  NA+ SP +    A  ++Y+ +G  R+Q+V    K   D E++ GQL
Sbjct: 350 NLVQMSAVKVNLYQNALLSPFWNIN-AHSVVYITQGRARVQVVNNNGKTVFDGELRRGQL 409

[Close]

BLAST of MS012501 vs. ExPASy TrEMBL
Match: A0A6J1JDB2 (12S seed storage protein CRD-like OS=Cucurbita maxima OX=3661 GN=LOC111483370 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.0e-159
Identity = 282/350 (80.57%), Postives = 314/350 (89.71%), Query Frame = 0


Query: 6   QPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLR 65
           QPM+PKPF E +AGSY KWLPS+YPLLA  KVAAGRLLLRP GFVVPHYAD SKVGYVL+
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 66  GGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPG 125
           G +GVAG VFP+KSDEVVV L++GDLIPVP GV+SWWFNDGDSD EI+FLGE+K+AHVPG
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 126 DFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKR 185
           D +YF+LSG L LL GFSPEYVG+TY+LNGEETT  LKSQSN LIFS+Q +QSLPKP K 
Sbjct: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182

Query: 186 SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEA 245
           SK VYNIDAAAPDGR K G G+VTTVTESKFPFIGQSGLTA+LEK++ANAVRSPVYVAE 
Sbjct: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242

Query: 246 ADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATH 305
            DQLIYVA+G G+IQIVGF+SKIDAEVK GQL+LVPK+FAVGKIAGE+GLECIS+ITATH
Sbjct: 243 YDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302

Query: 306 PVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           PVVE+LAGKT V EALSPEV QVSFNVTAEFEKLLRSKIT ASP++  SD
Sbjct: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 352

[Close]

BLAST of MS012501 vs. ExPASy TrEMBL
Match: A0A6J1E9P2 (legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.0e-159
Identity = 282/350 (80.57%), Postives = 313/350 (89.43%), Query Frame = 0


Query: 6   QPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVLR 65
           QPM+PKPF E +AGSY KWLPS+YPLLAQ KVAAGRLLLRP GFVVPHYAD SKVGYVL+
Sbjct: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62

Query: 66  GGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVPG 125
           G +GVAG VFP+KSDEVVV L++GDLIPVP GV+SWWFNDGDSD EI+FLGE+K+AHVPG
Sbjct: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122

Query: 126 DFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHKR 185
           D +YF+LSG L LL GFSPEYVGKTY+LNGEETT  LKSQSN LIFS+Q +QSLPKP K 
Sbjct: 123 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKY 182

Query: 186 SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAEA 245
           SK VYNIDAAAPDGR K G G+VTTVTESKFPFIGQSGLTA+LEK+ ANAVRSPVYVAE 
Sbjct: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVAEP 242

Query: 246 ADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITATH 305
            DQLIYVA+G G+IQIVG +SKIDAEVK GQL+LVPK+FAVGKIAGE+GLECIS+ITATH
Sbjct: 243 YDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302

Query: 306 PVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           PVVE+LAGKT V EALSPE+ QVSFNVTAEFEKLLRSKIT ASP++  SD
Sbjct: 303 PVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

[Close]

BLAST of MS012501 vs. ExPASy TrEMBL
Match: A0A5A7T7U8 (Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001330 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.5e-150
Identity = 261/340 (76.76%), Postives = 299/340 (87.94%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           ++ M+PKPFFEG+ GSY KWLPSDYPLLAQT VA GRLLLRP GF VPHYAD SK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 65  RGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVP 124
           +G DGV GFVFPNK +EVV+KL++GDLIPVP+G+TSWWFNDGDSD EI+FLGETK+AHVP
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 125 GDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHK 184
           GD TYFILSG  GLLQGF+PEYV K+Y+L+ EET   LKSQSN LIF++QPSQSLPKPHK
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHK 180

Query: 185 RSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAE 244
            SKLVYNIDAA PD R K G  +VT VTES FPFIGQ+GLTAVLEK++ANA+RSPVY+AE
Sbjct: 181 HSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIAE 240

Query: 245 AADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITAT 304
            +DQLIYV +GSG+IQ+VGF+SK DA+VK GQL+LVP+YFAVGK+AGEEGLECIS+I AT
Sbjct: 241 PSDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVAT 300

Query: 305 HPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKI 345
           HP+VE+LAGKT V EALS EV QVSFNVTAEFEKL RSK+
Sbjct: 301 HPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

[Close]

BLAST of MS012501 vs. ExPASy TrEMBL
Match: A0A1S3C2D5 (glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.5e-150
Identity = 261/340 (76.76%), Postives = 299/340 (87.94%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           ++ M+PKPFFEG+ GSY KWLPSDYPLLAQT VA GRLLLRP GF VPHYAD SK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVL 60

Query: 65  RGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVP 124
           +G DGV GFVFPNK +EVV+KL++GDLIPVP+G+TSWWFNDGDSD EI+FLGETK+AHVP
Sbjct: 61  QGEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVP 120

Query: 125 GDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHK 184
           GD TYFILSG  GLLQGF+PEYV K+Y+L+ EET   LKSQSN LIF++QPSQSLPKPHK
Sbjct: 121 GDITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHK 180

Query: 185 RSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAE 244
            SKLVYNIDAA PD R K G  +VT VTES FPFIGQ+GLTAVLEK++ANA+RSPVY+AE
Sbjct: 181 HSKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIAE 240

Query: 245 AADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITAT 304
            +DQLIYV +GSG+IQ+VGF+SK DA+VK GQL+LVP+YFAVGK+AGEEGLECIS+I AT
Sbjct: 241 PSDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVAT 300

Query: 305 HPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKI 345
           HP+VE+LAGKT V EALS EV QVSFNVTAEFEKL RSK+
Sbjct: 301 HPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

[Close]

BLAST of MS012501 vs. ExPASy TrEMBL
Match: A0A0A0L6K0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G218160 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 7.3e-150
Identity = 262/340 (77.06%), Postives = 297/340 (87.35%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           ++ M+PKPFFEG+ GSY KWLPSDYPLLAQT VA GRLLLRP GF VPHY+D SK GYVL
Sbjct: 1   MEAMNPKPFFEGEGGSYHKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYSDCSKFGYVL 60

Query: 65  RGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSAHVP 124
           +G DGV GFVFP K +EVV+KL++GDLIPVPAGVTSWWFNDGDSD EI+FLGETK AHVP
Sbjct: 61  QGEDGVTGFVFPKKCNEVVIKLKKGDLIPVPAGVTSWWFNDGDSDLEIIFLGETKRAHVP 120

Query: 125 GDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPKPHK 184
           GD TYFILSG  GLLQGF+PEYV K+ +LN EET T LKSQ N LIF++QPSQSLPKPHK
Sbjct: 121 GDITYFILSGPRGLLQGFTPEYVQKSCSLNQEETNTFLKSQPNVLIFTVQPSQSLPKPHK 180

Query: 185 RSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYVAE 244
            SKLVYNIDAAAPD R K G  +VT VTES FPFIGQ+GLT VLEK++ANA+RSPVY+AE
Sbjct: 181 YSKLVYNIDAAAPDNRAKVGDAAVTMVTESTFPFIGQTGLTPVLEKLDANAIRSPVYIAE 240

Query: 245 AADQLIYVAEGSGRIQIVGFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISVITAT 304
            +DQLIYV +GSG+IQ+VGF+SK DA+VK+GQL+LVP+YFAVGKIAGEEGLECIS+I AT
Sbjct: 241 PSDQLIYVTKGSGKIQVVGFSSKFDADVKTGQLILVPRYFAVGKIAGEEGLECISMIVAT 300

Query: 305 HPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKI 345
           HP+VE+LAGKT V EALS EV QVSFNVTAEFEKL RSK+
Sbjct: 301 HPMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

[Close]

BLAST of MS012501 vs. TAIR 10
Match: AT1G07750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 9.3e-73
Identity = 140/357 (39.22%), Postives = 212/357 (59.38%), Query Frame = 0


Query: 2   QLNLQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVG 61
           +L+L P  PK  + GD GSY  W P + P+L Q  + A +L L   GF VP Y+DSSKV 
Sbjct: 2   ELDLTPKLPKKVYGGDGGSYSAWCPEELPMLKQGNIGAAKLALEKNGFAVPRYSDSSKVA 61

Query: 62  YVLRGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSA 121
           YVL+ G G AG V P K +E V+ +++GD I +P GV +WWFN+ D +  I+FLGET   
Sbjct: 62  YVLQ-GSGTAGIVLPEK-EEKVIAIKQGDSIALPFGVVTWWFNNEDPELVILFLGETHKG 121

Query: 122 HVPGDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPK 181
           H  G FT F L+G+ G+  GFS E+VG+ ++L+      L+ SQ+   I  L     +P+
Sbjct: 122 HKAGQFTEFYLTGTNGIFTGFSTEFVGRAWDLDENTVKKLVGSQTGNGIVKLDAGFKMPQ 181

Query: 182 PHKRSKLVYNIDA-AAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPV 241
           P + ++  + ++   AP       GG V  +     P +G+ G  A L +I+A+++ SP 
Sbjct: 182 PKEENRAGFVLNCLEAPLDVDIKDGGRVVVLNTKNLPLVGEVGFGADLVRIDAHSMCSPG 241

Query: 242 YVAEAADQLIYVAEGSGRIQIVGFASK--IDAEVKSGQLLLVPKYFAVGKIAGEEGLECI 301
           +  ++A Q+ Y+  GSGR+Q+VG   K  ++  +K+G L +VP++F V KIA  +G+   
Sbjct: 242 FSCDSALQVTYIVGGSGRVQVVGGDGKRVLETHIKAGSLFIVPRFFVVSKIADADGMSWF 301

Query: 302 SVITATHPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
           S++T   P+   LAG T V+++LSPEV+Q +F V  E EK  RS  T ++   PPS+
Sbjct: 302 SIVTTPDPIFTHLAGNTSVWKSLSPEVLQAAFKVAPEVEKSFRSTRTSSAIFFPPSN 356

[Close]

BLAST of MS012501 vs. TAIR 10
Match: AT2G28680.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.1e-70
Identity = 144/358 (40.22%), Postives = 209/358 (58.38%), Query Frame = 0


Query: 2   QLNLQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVG 61
           +L+L P  PK  + GD GSY  W P + P+L    + A +L L   G  +P Y+DS KV 
Sbjct: 2   ELDLSPRLPKKVYGGDGGSYFAWCPEELPMLRDGNIGASKLALEKYGLALPRYSDSPKVA 61

Query: 62  YVLRGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVTSWWFNDGDSDFEIVFLGETKSA 121
           YVL+G  G AG V P K +E V+ +++GD I +P GV +WWFN+ D++  ++FLGET   
Sbjct: 62  YVLQGA-GTAGIVLPEK-EEKVIAIKKGDSIALPFGVVTWWFNNEDTELVVLFLGETHKG 121

Query: 122 HVPGDFTYFILSGSLGLLQGFSPEYVGKTYNLNGEETTTLLKSQSNGLIFSLQPSQSLPK 181
           H  G FT F L+GS G+  GFS E+VG+ ++L+      L+ SQ+   I  +  S  +P+
Sbjct: 122 HKAGQFTDFYLTGSNGIFTGFSTEFVGRAWDLDETTVKKLVGSQTGNGIVKVDASLKMPE 181

Query: 182 PHK--RSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSP 241
           P K  R   V N   A  D   K  GG V  +     P +G+ G  A L +I+ +++ SP
Sbjct: 182 PKKGDRKGFVLNCLEAPLDVDIK-DGGRVVVLNTKNLPLVGEVGFGADLVRIDGHSMCSP 241

Query: 242 VYVAEAADQLIYVAEGSGRIQIVGFASK--IDAEVKSGQLLLVPKYFAVGKIAGEEGLEC 301
            +  ++A Q+ Y+  GSGR+QIVG   K  ++  VK+G L +VP++F V KIA  +GL  
Sbjct: 242 GFSCDSALQVTYIVGGSGRVQIVGADGKRVLETHVKAGVLFIVPRFFVVSKIADSDGLSW 301

Query: 302 ISVITATHPVVEDLAGKTCVYEALSPEVVQVSFNVTAEFEKLLRSKITKASPLVPPSD 356
            S++T   P+   LAG+T V++ALSPEV+Q +F V  E EK  RSK T  +    PS+
Sbjct: 302 FSIVTTPDPIFTHLAGRTSVWKALSPEVLQAAFKVDPEVEKAFRSKRTSDAIFFSPSN 356

[Close]

BLAST of MS012501 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 3.7e-21
Identity = 97/416 (23.32%), Postives = 164/416 (39.42%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           +  ++P    + +AG  + W     P L    V   R+ L+P    +P +     + YV+
Sbjct: 41  INSLAPAQATKFEAGQMEVW-DHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVV 100

Query: 65  -------------------------RGGDGVAGFVFPNKSDEVVVKLREGDLIPVPAGVT 124
                                    RGG G  G  F +   + +   R GD+    AGV+
Sbjct: 101 QGEGVMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDMHQK-LENFRRGDVFASLAGVS 160

Query: 125 SWWFNDGDSDFEIVFL-----GETKSAHVPGDFTYFILSGSL--------------GLLQ 184
            WW+N GDSD  IV +      E +   VP     F L+GS                   
Sbjct: 161 QWWYNRGDSDAVIVIVLDVTNRENQLDQVP---RMFQLAGSRTQEEEQPLTWPSGNNAFS 220

Query: 185 GFSPEYVGKTYNLNGEETTTLLKSQSN---------GLIFSLQP---------SQSLPKP 244
           GF P  + + + +N E    L   + N          L F + P         +  + + 
Sbjct: 221 GFDPNIIAEAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEET 280

Query: 245 HKRSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPVYV 304
           +  +K+  NID            G ++T+     P +    L A+   + +  +  P + 
Sbjct: 281 YCTAKIHENIDDPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWT 340

Query: 305 AEAADQLIYVAEGSGRIQIV--GFASKIDAEVKSGQLLLVPKYFAVGKIAGEEGLECISV 357
           A  A  ++YV  G  +IQ+V     S  + +V  GQ++++P+ FAV K AGE G E IS 
Sbjct: 341 AN-AHTVLYVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISF 400

[Close]

BLAST of MS012501 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-20
Identity = 98/417 (23.50%), Postives = 170/417 (40.77%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           L  + P    + + G  + W     P L  +  A  R ++ P G  +P + ++ K+ +V+
Sbjct: 35  LNALEPSQIIKSEGGRIEVW-DHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVV 94

Query: 65  RGGDGVAGFVFPNKSD------------------------EVVVKLREGDLIPVPAGVTS 124
             G G+ G V P  ++                        + V  LR GD I  P+GV  
Sbjct: 95  H-GRGLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQ 154

Query: 125 WWFNDGDSDFEIVFLGETKS--AHVPGDFTYFILSGS----------------LGLLQGF 184
           W++N+G+    +V   +  S    +  +   F+++G+                  +  GF
Sbjct: 155 WFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGF 214

Query: 185 SPEYVGKTYNLNGEETTTLLKSQSN-----------GLIF-SLQPSQSLPKPHK------ 244
           +PE + + + +N E    L   Q N           G+I   L+  +   +PH+      
Sbjct: 215 APEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLE 274

Query: 245 ----RSKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEANAVRSPV 304
                 +   N+D  +     K   G ++T+     P +    L+A+   I  NA+  P 
Sbjct: 275 ETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQ 334

Query: 305 YVAEAADQLIYVAEGSGRIQIVGFASK--IDAEVKSGQLLLVPKYFAVGKIAGEEGLECI 351
           +    A+  +YV  G   IQ+V    +   D E+ SGQLL+VP+ F+V K A  E  E I
Sbjct: 335 WNVN-ANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWI 394

[Close]

BLAST of MS012501 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-16
Identity = 90/404 (22.28%), Postives = 155/404 (38.37%), Query Frame = 0


Query: 5   LQPMSPKPFFEGDAGSYQKWLPSDYPLLAQTKVAAGRLLLRPGGFVVPHYADSSKVGYVL 64
           L  + P    + +AG  + W     P L  + V+  R ++   G  +P + +++K+ +V 
Sbjct: 41  LNALEPSHVLKSEAGRIEVW-DHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAKLSFVA 100

Query: 65  RGGDGVAGFVFPNKSD-------------------------EVVVKLREGDLIPVPAGVT 124
           + G G+ G V P  ++                         + V  +R GD I    GV 
Sbjct: 101 K-GRGLMGKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVA 160

Query: 125 SWWFNDGDSDFEIVFLGETKSAHVPGDFT--YFILSGS----------------LGLLQG 184
            W++NDG     IV + +  S     D     F L+G+                  +  G
Sbjct: 161 QWFYNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQKNIFNG 220

Query: 185 FSPEYVGKTYNLNGEETTTLLKSQSN-GLIFSLQPSQSLPKPHKR--------------- 244
           F PE + +   ++ +    L     N G I  +Q    + +P  R               
Sbjct: 221 FGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRHG 280

Query: 245 ------------SKLVYNIDAAAPDGRTKAGGGSVTTVTESKFPFIGQSGLTAVLEKIEA 304
                       ++   N+D  +     K   G ++T+     P +    L+A+   I  
Sbjct: 281 RHGNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQ 340

Query: 305 NAVRSPVYVAEAADQLIYVAEGSGRIQIVGFASK--IDAEVKSGQLLLVPKYFAVGKIAG 336
           NA+  P + A  A+ ++YV +G  +IQIV        D +V  GQL+ VP+ F+V K A 
Sbjct: 341 NAMVLPQWNAN-ANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVKRAT 400

The following BLAST results are available for this feature:

BLAST of MS012501 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922755.1	1.0e-158	80.57	legumin J-like [Cucurbita moschata]	[more]
XP_022985328.1	1.0e-158	80.57	12S seed storage protein CRD-like [Cucurbita maxima]	[more]
KAG6576976.1	6.7e-158	80.00	12S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023552908.1	8.2e-156	79.43	12S seed storage globulin 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_008456076.1	5.2e-150	76.76	PREDICTED: glutelin type-A 2-like [Cucumis melo] >KAA0039043.1 glutelin type-A 2...	[more]

BLAST of MS012501 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23880	1.0e-20	24.24	13S globulin seed storage protein 2 OS=Fagopyrum esculentum OX=3617 GN=FA18 PE=2...	[more]
Q02897	3.1e-20	21.99	Glutelin type-B 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUB2 PE=2 SV=2	[more]
P14323	4.0e-20	22.54	Glutelin type-B 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GluB1-A PE=2 SV=3	[more]
Q9ZWA9	5.2e-20	23.32	12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1	[more]
Q09151	6.8e-20	23.58	Glutelin type-A 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA3 PE=2 SV=2	[more]

BLAST of MS012501 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDB2	5.0e-159	80.57	12S seed storage protein CRD-like OS=Cucurbita maxima OX=3661 GN=LOC111483370 PE...	[more]
A0A6J1E9P2	5.0e-159	80.57	legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1	[more]
A0A5A7T7U8	2.5e-150	76.76	Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8...	[more]
A0A1S3C2D5	2.5e-150	76.76	glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1	[more]
A0A0A0L6K0	7.3e-150	77.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G218160 PE=4 SV=1	[more]

BLAST of MS012501 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07750.1	9.3e-73	39.22	RmlC-like cupins superfamily protein	[more]
AT2G28680.1	1.1e-70	40.22	RmlC-like cupins superfamily protein	[more]
AT1G03890.1	3.7e-21	23.32	RmlC-like cupins superfamily protein	[more]
AT1G03880.1	1.8e-20	23.50	cruciferin 2	[more]
AT5G44120.3	2.7e-16	22.28	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 3..159 e-value: 1.5E-34 score: 130.7 coord: 190..338 e-value: 8.2E-16 score: 68.5
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 195..336 e-value: 6.5E-19 score: 68.0 coord: 9..158 e-value: 9.5E-23 score: 80.4
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 8..179 e-value: 2.1E-32 score: 113.8
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 195..356 e-value: 8.9E-38 score: 131.2
None	No IPR available	PANTHER	PTHR31189	OS03G0336100 PROTEIN-RELATED	coord: 3..346
None	No IPR available	PANTHER	PTHR31189:SF45	11S GLOBULIN SEED STORAGE PROTEIN 2-LIKE	coord: 3..346
None	No IPR available	CDD	cd02242	cupin_11S_legumin_N	coord: 4..175 e-value: 5.95851E-62 score: 195.497
None	No IPR available	CDD	cd02243	cupin_11S_legumin_C	coord: 204..352 e-value: 1.41097E-59 score: 187.296
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 5..341

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012501.1	MS012501.1	mRNA