MS012496 (gene) Bitter gourd (TR) v1

Overview


Name	MS012496
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	nigrin b-like
Location	scaffold63: 1097835 .. 1098701 (-)
RNA-Seq Expression	MS012496
Synteny	MS012496

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAGGGATGGTGGTCTACATTGTTGTCGCACTTTCAGTACTAATGAGCGTCCATCGTGGCATGGCGGCCTATCCATATCCTAGGTATGGAAGCTTCGGAAAAATATCACATATTGTAGGTCGAGATGGAATGTGTTTGGACATTGCTGTTCCCAATGTCCGACCACTTTACGCTCAAACTCAATTATCGCGGTGTGACGTTGCGAAACAGACCCAAATGTGGAGCATCCTTGAAGATGGCACCATTCGAACCAAGGACAACGTATATTGCTTGGTTCCTTACAACGATGCGTATGAAGCCAAAGCCGTATTGGGCAAGTGCTCTGAATTGATAAGAAGTGACAAGAAATGGAAGCATAGGAGTGATGGCACTTTTGTCCACGAGAACTCGGGCTTCGTCCTGACAGCGAAACCAGACAACTTCGTGATATTGGGAACTGACGCATACACCCCATCCCAAAGCTGGGAAGCCACCGAGGGTCTCTCCCCTTCTGTGGTTAACATCAAGTGGCTTGAAAACCAGTGTTTGCAGTCCATGGATAACGGTGCTCCCTTTGTGTATTTGAATCTATGTGATAAAAAAAATAAGAACCAACATTGGGCACTATATTCTGACGGCACCATCCGTGTTGATAACTATGGCAACAGAGACTACTGCTTGACGTCTCAAAGAAATCCATCTAGTGTCATCCTTGTGTCTAACTGTGACGACAAGGCGACACAACGATGGGATATTAATGAAGACAACTTCATTTACCATCCCAACACTAACTTGGTCTTGGATGTCGATAGAAAACCTGAGGGATCCCAAATTGTCGTTATCGACACTCGTGATGGCACTCCTACCCAGCAATGGACGATACTC

mRNA sequence

ATGAGAGGGATGGTGGTCTACATTGTTGTCGCACTTTCAGTACTAATGAGCGTCCATCGTGGCATGGCGGCCTATCCATATCCTAGGTATGGAAGCTTCGGAAAAATATCACATATTGTAGGTCGAGATGGAATGTGTTTGGACATTGCTGTTCCCAATGTCCGACCACTTTACGCTCAAACTCAATTATCGCGGTGTGACGTTGCGAAACAGACCCAAATGTGGAGCATCCTTGAAGATGGCACCATTCGAACCAAGGACAACGTATATTGCTTGGTTCCTTACAACGATGCGTATGAAGCCAAAGCCGTATTGGGCAAGTGCTCTGAATTGATAAGAAGTGACAAGAAATGGAAGCATAGGAGTGATGGCACTTTTGTCCACGAGAACTCGGGCTTCGTCCTGACAGCGAAACCAGACAACTTCGTGATATTGGGAACTGACGCATACACCCCATCCCAAAGCTGGGAAGCCACCGAGGGTCTCTCCCCTTCTGTGGTTAACATCAAGTGGCTTGAAAACCAGTGTTTGCAGTCCATGGATAACGGTGCTCCCTTTGTGTATTTGAATCTATGTGATAAAAAAAATAAGAACCAACATTGGGCACTATATTCTGACGGCACCATCCGTGTTGATAACTATGGCAACAGAGACTACTGCTTGACGTCTCAAAGAAATCCATCTAGTGTCATCCTTGTGTCTAACTGTGACGACAAGGCGACACAACGATGGGATATTAATGAAGACAACTTCATTTACCATCCCAACACTAACTTGGTCTTGGATGTCGATAGAAAACCTGAGGGATCCCAAATTGTCGTTATCGACACTCGTGATGGCACTCCTACCCAGCAATGGACGATACTC

Coding sequence (CDS)

ATGAGAGGGATGGTGGTCTACATTGTTGTCGCACTTTCAGTACTAATGAGCGTCCATCGTGGCATGGCGGCCTATCCATATCCTAGGTATGGAAGCTTCGGAAAAATATCACATATTGTAGGTCGAGATGGAATGTGTTTGGACATTGCTGTTCCCAATGTCCGACCACTTTACGCTCAAACTCAATTATCGCGGTGTGACGTTGCGAAACAGACCCAAATGTGGAGCATCCTTGAAGATGGCACCATTCGAACCAAGGACAACGTATATTGCTTGGTTCCTTACAACGATGCGTATGAAGCCAAAGCCGTATTGGGCAAGTGCTCTGAATTGATAAGAAGTGACAAGAAATGGAAGCATAGGAGTGATGGCACTTTTGTCCACGAGAACTCGGGCTTCGTCCTGACAGCGAAACCAGACAACTTCGTGATATTGGGAACTGACGCATACACCCCATCCCAAAGCTGGGAAGCCACCGAGGGTCTCTCCCCTTCTGTGGTTAACATCAAGTGGCTTGAAAACCAGTGTTTGCAGTCCATGGATAACGGTGCTCCCTTTGTGTATTTGAATCTATGTGATAAAAAAAATAAGAACCAACATTGGGCACTATATTCTGACGGCACCATCCGTGTTGATAACTATGGCAACAGAGACTACTGCTTGACGTCTCAAAGAAATCCATCTAGTGTCATCCTTGTGTCTAACTGTGACGACAAGGCGACACAACGATGGGATATTAATGAAGACAACTTCATTTACCATCCCAACACTAACTTGGTCTTGGATGTCGATAGAAAACCTGAGGGATCCCAAATTGTCGTTATCGACACTCGTGATGGCACTCCTACCCAGCAATGGACGATACTC

Protein sequence

MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLTSQRNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL

Homology

[Close]

BLAST of MS012496 vs. NCBI nr
Match: XP_022149160.1 (nigrin b-like [Momordica charantia])

HSP 1 Score: 598.6 bits (1542), Expect = 2.9e-167
Identity = 284/289 (98.27%), Postives = 287/289 (99.31%), Query Frame = 0


Query: 1   MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQ 60
           MRGMVVYIVVALSVLMSV+RGMAAYPYP YGSFGKISHIVGRDGMCLDI VPNVRPLYAQ
Sbjct: 5   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLSRCDVAKQTQMWSILEDGTIRTKDN+YCLVPYNDAYEAKAVLGKCSELIRSDKKWKH
Sbjct: 65  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM
Sbjct: 125 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLTSQRNPSSVILVSNCDDKA 240
           DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYG+RDYCLTSQRNPSSVILVSNCDDKA
Sbjct: 185 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA 244

Query: 241 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL 290
           TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL
Sbjct: 245 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL 293

[Close]

BLAST of MS012496 vs. NCBI nr
Match: XP_022149159.1 (beta-galactoside-specific lectin 3-like [Momordica charantia])

HSP 1 Score: 515.8 bits (1327), Expect = 2.5e-142
Identity = 246/290 (84.83%), Postives = 261/290 (90.00%), Query Frame = 0


Query: 1   MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQ 60
           M+ MVV IVVALSVLMS H  MAAYPYPRYGSFGK SHIVGRDG+CLDIA      +Y Q
Sbjct: 5   MKEMVVCIVVALSVLMSAHHAMAAYPYPRYGSFGKKSHIVGRDGLCLDIAKFEPAQVYIQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLS C+ AKQTQ+W+ILEDGTIRTKD VYCLVPYNDAYEAKAVLGKC+EL+RS KKW H
Sbjct: 65  TQLSLCEAAKQTQLWTILEDGTIRTKDYVYCLVPYNDAYEAKAVLGKCAELMRSVKKWNH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           ++DGTFVHEN+G VLTAKPDNFV+LGTD  TPSQSWEATEGL+PSVVNI+W E  CLQSM
Sbjct: 125 KNDGTFVHENTGLVLTAKPDNFVVLGTDVNTPSQSWEATEGLTPSVVNIQWREGLCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNR-DYCLTSQRNPSSVILVSNCDDK 240
           DNGAPFVYLN CDKKNKNQHWALYSDGTIRVDNYG R DYCLTSQRNPSSVILVSNCDDK
Sbjct: 185 DNGAPFVYLNSCDKKNKNQHWALYSDGTIRVDNYGKRSDYCLTSQRNPSSVILVSNCDDK 244

Query: 241 ATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL 290
           ATQRW INE+NFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTP QQWTIL
Sbjct: 245 ATQRWGINEENFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPNQQWTIL 294

[Close]

BLAST of MS012496 vs. NCBI nr
Match: XP_023551012.1 (nigrin b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265.0 bits (676), Expect = 7.6e-67
Identity = 140/288 (48.61%), Postives = 180/288 (62.50%), Query Frame = 0


Query: 6   VYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQTQLSR 65
           V   +AL+  ++ H  +AA     YGSFG  SH+VGRDG+CL+  +  +   Y  T LS 
Sbjct: 7   VLFCIALAFSLATHTAIAA--ASGYGSFGARSHLVGRDGLCLNALL--LFGYYTPTDLSL 66

Query: 66  CDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRS 125
           C+  K  Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS  IR D KW    
Sbjct: 67  CEQRKSNQLWSILEDGTIRISDVRFCLVPKSQSTEFIGANVVAMDCSREIRHDMKWTKNK 126

Query: 126 DGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDN 185
           DGT  H+ SG VLTAKP   V +  +   PSQSWEATE  +P V NIKWL+N CL+S++ 
Sbjct: 127 DGTIHHDESGLVLTAKPGKTVTVEVNENLPSQSWEATETFNPMVANIKWLDNLCLKSIEG 186

Query: 186 GAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLTS-QRNPSSVILVSNCDDKAT 245
              +V L  C + +KNQ WALY DGTIR + Y  R  CLTS +RN  ++++VS C DK  
Sbjct: 187 ENSYVTLKECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSFERNYGAMVVVSRCADKPQ 246

Query: 246 QRWDINEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 289
           QRW + EDN I HPNTNLV+DV R+ P    ++V++  DG P+QQWTI
Sbjct: 247 QRWGLAEDNTINHPNTNLVMDVRREVPLLPPMIVVEKHDGAPSQQWTI 288

[Close]

BLAST of MS012496 vs. NCBI nr
Match: XP_022941584.1 (nigrin b-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 264.2 bits (674), Expect = 1.3e-66
Identity = 139/288 (48.26%), Postives = 182/288 (63.19%), Query Frame = 0


Query: 6   VYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQTQLSR 65
           V   +AL+  ++ H  +AA     YGSFG  +H+VGRDG+CLD  +  +   Y  T LS+
Sbjct: 7   VLFCIALAFSLATHTAIAA--ASGYGSFGSRTHLVGRDGLCLDTML--LLGYYPPTHLSQ 66

Query: 66  CDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRS 125
           C+  K +Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS+ IR D KW    
Sbjct: 67  CEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNK 126

Query: 126 DGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDN 185
           DGT  H+ SG VLTAKP   V +  +   PSQSWEATE   P V NIKWL+N CL+S++ 
Sbjct: 127 DGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEG 186

Query: 186 GAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLT-SQRNPSSVILVSNCDDKAT 245
              +V LN C + +KNQ WALY DGTIR + Y  R  CLT S+RN  ++++VS C DK  
Sbjct: 187 ENSYVTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGAMVVVSRCADKPQ 246

Query: 246 QRWDINEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 289
           QRW + ED+ I HPNTNLV+DV R+ P    ++V+D  DG  +Q+WTI
Sbjct: 247 QRWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 288

[Close]

BLAST of MS012496 vs. NCBI nr
Match: XP_022982216.1 (ribosome-inactivating protein SNAI-like [Cucurbita maxima])

HSP 1 Score: 261.9 bits (668), Expect = 6.4e-66
Identity = 137/293 (46.76%), Postives = 183/293 (62.46%), Query Frame = 0


Query: 1   MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQ 60
           MR +++ I +A S+ ++            YG+FG  +H+VGRDG+CLD  +  +   Y Q
Sbjct: 4   MRQVLLCIALAFSLAIAAP--------SEYGAFGGRTHLVGRDGLCLDTML--LSGYYPQ 63

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVP---YNDAYEAKAVLGKCSELIRSDKK 120
           T LS+C   K +Q+W+ILEDGTIR  D  YCLVP   +        V+  C++ +R D K
Sbjct: 64  TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDMK 123

Query: 121 WKHRSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCL 180
           W  + DGT  H+ SG VLTAKP   V L  +   PSQSWEATE L P V NIKWL+N CL
Sbjct: 124 WTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCL 183

Query: 181 QSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLT-SQRNPSSVILVSNC 240
           +S +    +V LN C + +KNQ WALY DGTIR + Y  R  CLT S+RN  ++++VS C
Sbjct: 184 KSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGALVVVSRC 243

Query: 241 DDKATQRWDINEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 289
            DK  QRW + EDN I HPNTNLV+DV R+ P    ++V+D  DG P+Q+W+I
Sbjct: 244 ADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPMIVVDKHDGAPSQRWSI 284

[Close]

BLAST of MS012496 vs. ExPASy Swiss-Prot
Match: P82683 (Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 2.7e-27
Identity = 82/257 (31.91%), Postives = 124/257 (48.25%), Query Frame = 0


Query: 39  IVGRDGMCLDI---AVPNVRPLYAQTQLSRC-DVAKQTQMWSILEDGTIRTKDNVYCLVP 98
           IVGRDG+C+D+      N  P+    QLS C       Q+W+I  DGTIR+  N  CL  
Sbjct: 320 IVGRDGLCVDVRDGKFHNGNPI----QLSPCKSNTDPNQLWTIRRDGTIRS--NGRCLTT 379

Query: 99  YNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTA---KPDNFVILGTDAYT 158
           Y        ++  C+  +R    W+   +GT ++  S  VL A        + + T  Y+
Sbjct: 380 YGYTAGVYVMIFDCNTAVREATLWQIWGNGTIINPRSNLVLGAASGSSGTTLTVQTQVYS 439

Query: 159 PSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRV 218
             Q W A    +P  V I    + C+++  NGA  V++  C    +NQ+WALY DG+IR 
Sbjct: 440 LGQGWLAGNDTAPREVTIYGFRDLCMEA--NGAS-VWVETCGSSTENQNWALYGDGSIRP 499

Query: 219 DNYGNRDYCLTSQRNP-SSVILVSNCD-DKATQRWDINEDNFIYHPNTNLVLDVDRKPEG 278
               N+D CLT Q +  ++VI + +C    + QRW    +  I + N  LV+DV +    
Sbjct: 500 KQ--NQDQCLTCQGDSVATVINIVSCSAGSSGQRWVFTNEGTILNLNNGLVMDVAQSNPS 559

Query: 279 SQIVVIDTRDGTPTQQW 287
            + ++I    G P Q W
Sbjct: 560 LRRIIIYPATGNPNQMW 565

[Close]

BLAST of MS012496 vs. ExPASy Swiss-Prot
Match: P28590 (Abrin-c OS=Abrus precatorius OX=3816 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.3e-26
Identity = 74/256 (28.91%), Postives = 123/256 (48.05%), Query Frame = 0


Query: 39  IVGRDGMCLDIAVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDA 98
           I GRDGMC+D+                 D  ++ Q+W++  D TIR+  N  CL     A
Sbjct: 313 IGGRDGMCVDVYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRS--NGKCLTTEGYA 372

Query: 99  YEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNF---VILGTDAYTPSQS 158
                ++  C+  +     W+   +GT ++  S  VL+A+  +    + + T+ Y   Q 
Sbjct: 373 PGNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQG 432

Query: 159 WEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYG 218
           W      SP V +I    + C+Q+  +    V+L  CD   K Q WALY+DG+IR  +  
Sbjct: 433 WRTGNNTSPFVTSISGYSDLCMQAQGSN---VWLADCDNNKKEQQWALYTDGSIR--SVQ 492

Query: 219 NRDYCLTSQ--RNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIV 278
           N + CLTS+  +  S ++L++  +  A+QRW    D  IY+ + ++V+DV R     + +
Sbjct: 493 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFKNDGSIYNLHDDMVMDVKRSDPSLKEI 552

Query: 279 VIDTRDGTPTQQWTIL 290
           ++    G P Q W  L
Sbjct: 553 ILHPYHGKPNQIWLTL 561

[Close]

BLAST of MS012496 vs. ExPASy Swiss-Prot
Match: Q06077 (Abrin-b OS=Abrus precatorius OX=3816 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.9e-26
Identity = 73/256 (28.52%), Postives = 123/256 (48.05%), Query Frame = 0


Query: 39  IVGRDGMCLDIAVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDA 98
           I GR+GMC+D+                 D  ++ Q+W++  D TIR+  N  CL     A
Sbjct: 278 IGGRNGMCVDVYDDGYHNGNRIIAWKCKDRLEENQLWTLKSDKTIRS--NGKCLTTEGYA 337

Query: 99  YEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNF---VILGTDAYTPSQS 158
                ++  C+  +     W+   +GT ++  S  VL+A+  +    + + T+ Y   Q 
Sbjct: 338 PGNYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQG 397

Query: 159 WEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYG 218
           W      SP V +I    + C+Q+  +    V+L  CD   K Q WALY+DG+IR  +  
Sbjct: 398 WRTGNNTSPFVTSISGYSDLCMQAQGSN---VWLAYCDNNKKEQQWALYTDGSIR--SVQ 457

Query: 219 NRDYCLTSQ--RNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIV 278
           N + CLTS+  +  S ++L++  +  A+QRW    D  IY+ + ++V+DV R     + +
Sbjct: 458 NTNNCLTSKDHKQGSPIVLMACSNGWASQRWLFRNDGSIYNLHDDMVMDVKRSDPSLKEI 517

Query: 279 VIDTRDGTPTQQWTIL 290
           ++    G P Q W  L
Sbjct: 518 ILHPYHGKPNQIWLTL 526

[Close]

BLAST of MS012496 vs. ExPASy Swiss-Prot
Match: P11140 (Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 5.2e-26
Identity = 75/257 (29.18%), Postives = 127/257 (49.42%), Query Frame = 0


Query: 39  IVGRDGMCLDIAVPNVRPLYAQTQLSRC-DVAKQTQMWSILEDGTIRTKDNVYCLVPYND 98
           I GRDGMC+D+   N      +  + +C D  ++ Q+W++  D TIR+  N  CL  Y  
Sbjct: 279 IGGRDGMCVDV-YDNGYHNGNRIIMWKCKDRLEENQLWTLKSDKTIRS--NGKCLTTYGY 338

Query: 99  AYEAKAVLGKCSELIRSDKKWKHRSDGTFVHENSGFVLTAKPDNF---VILGTDAYTPSQ 158
           A  +  ++  C+  +     W+   +GT ++  S  VL+A+  +    + + T+ Y   Q
Sbjct: 339 APGSYVMIYDCTSAVAEATYWEIWDNGTIINPKSALVLSAESSSMGGTLTVQTNEYLMRQ 398

Query: 159 SWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNY 218
            W      SP V +I    + C+Q+  +    V++  CD   K Q WALY+DG+IR  + 
Sbjct: 399 GWRTGNNTSPFVTSISGYSDLCMQAQGSN---VWMADCDSNKKEQQWALYTDGSIR--SV 458

Query: 219 GNRDYCLTSQ--RNPSSVILVSNCDDKATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQI 278
            N + CLTS+  +  S+++L+   +  A+QRW    D  IY    ++V+DV       + 
Sbjct: 459 QNTNNCLTSKDHKQGSTILLMGCSNGWASQRWVFKNDGSIYSLYDDMVMDVKGSDPSLKQ 518

Query: 279 VVIDTRDGTPTQQWTIL 290
           +++    G P Q W  L
Sbjct: 519 IILWPYTGKPNQIWLTL 527

[Close]

BLAST of MS012496 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 6.7e-26
Identity = 80/262 (30.53%), Postives = 123/262 (46.95%), Query Frame = 0


Query: 35  KISHIVGRDGMCLDIAVPNVRPLYAQTQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVP 94
           + + I GRD +C+D+A   +    ++  L  C   +  Q W+   DGT+R+     CL  
Sbjct: 263 RTTRISGRDALCVDVA-GALTSDGSRLILYPCG-QQVNQKWTFHSDGTVRSLGK--CLAT 322

Query: 95  YNDAYEAKAVLGKCSELIRSDKKWKHRSDGTFVHEN-SGFVLT---AKPDNFVILGTDAY 154
            N  +    V+  CS+L   D  W     GT ++ N     LT   A     + +  + Y
Sbjct: 323 NNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIMNPNYEDLALTSNKATRSTNLTMEVNTY 382

Query: 155 TPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIR 214
           + SQ W     + P + +I  L++ CL++ D G   ++L  C    + Q WALYSDGTIR
Sbjct: 383 SASQGWRVGNYVQPIIGSIVGLDDMCLEATD-GNTNMWLEECVPNQREQSWALYSDGTIR 442

Query: 215 VDNYGNRDYCLTSQRNPS---SVILVSNCDDKATQRWDINEDNFIYHP-NTNLVLDVDRK 274
           VD+  NR+ C+T+  +      VI + NCD    QRW    D  I  P N  L +DV R 
Sbjct: 443 VDD--NRELCVTASSSTYDNWKVITILNCDGSNNQRWVFLADGSISTPGNQRLAMDVARS 502

Query: 275 PEGSQIVVIDTRDGTPTQQWTI 289
               + +++    G   QQW +
Sbjct: 503 DVDLKKIILHRPHGDLNQQWVL 517

[Close]

BLAST of MS012496 vs. ExPASy TrEMBL
Match: A0A6J1D4Y7 (nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111017650 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.4e-167
Identity = 284/289 (98.27%), Postives = 287/289 (99.31%), Query Frame = 0


Query: 1   MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQ 60
           MRGMVVYIVVALSVLMSV+RGMAAYPYP YGSFGKISHIVGRDGMCLDI VPNVRPLYAQ
Sbjct: 5   MRGMVVYIVVALSVLMSVYRGMAAYPYPGYGSFGKISHIVGRDGMCLDIVVPNVRPLYAQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLSRCDVAKQTQMWSILEDGTIRTKDN+YCLVPYNDAYEAKAVLGKCSELIRSDKKWKH
Sbjct: 65  TQLSRCDVAKQTQMWSILEDGTIRTKDNLYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM
Sbjct: 125 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLTSQRNPSSVILVSNCDDKA 240
           DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYG+RDYCLTSQRNPSSVILVSNCDDKA
Sbjct: 185 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGSRDYCLTSQRNPSSVILVSNCDDKA 244

Query: 241 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL 290
           TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL
Sbjct: 245 TQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL 293

[Close]

BLAST of MS012496 vs. ExPASy TrEMBL
Match: A0A6J1D675 (beta-galactoside-specific lectin 3-like OS=Momordica charantia OX=3673 GN=LOC111017649 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.2e-142
Identity = 246/290 (84.83%), Postives = 261/290 (90.00%), Query Frame = 0


Query: 1   MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQ 60
           M+ MVV IVVALSVLMS H  MAAYPYPRYGSFGK SHIVGRDG+CLDIA      +Y Q
Sbjct: 5   MKEMVVCIVVALSVLMSAHHAMAAYPYPRYGSFGKKSHIVGRDGLCLDIAKFEPAQVYIQ 64

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYEAKAVLGKCSELIRSDKKWKH 120
           TQLS C+ AKQTQ+W+ILEDGTIRTKD VYCLVPYNDAYEAKAVLGKC+EL+RS KKW H
Sbjct: 65  TQLSLCEAAKQTQLWTILEDGTIRTKDYVYCLVPYNDAYEAKAVLGKCAELMRSVKKWNH 124

Query: 121 RSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSM 180
           ++DGTFVHEN+G VLTAKPDNFV+LGTD  TPSQSWEATEGL+PSVVNI+W E  CLQSM
Sbjct: 125 KNDGTFVHENTGLVLTAKPDNFVVLGTDVNTPSQSWEATEGLTPSVVNIQWREGLCLQSM 184

Query: 181 DNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNR-DYCLTSQRNPSSVILVSNCDDK 240
           DNGAPFVYLN CDKKNKNQHWALYSDGTIRVDNYG R DYCLTSQRNPSSVILVSNCDDK
Sbjct: 185 DNGAPFVYLNSCDKKNKNQHWALYSDGTIRVDNYGKRSDYCLTSQRNPSSVILVSNCDDK 244

Query: 241 ATQRWDINEDNFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPTQQWTIL 290
           ATQRW INE+NFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTP QQWTIL
Sbjct: 245 ATQRWGINEENFIYHPNTNLVLDVDRKPEGSQIVVIDTRDGTPNQQWTIL 294

[Close]

BLAST of MS012496 vs. ExPASy TrEMBL
Match: A0A6J1FNV0 (nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 6.2e-67
Identity = 139/288 (48.26%), Postives = 182/288 (63.19%), Query Frame = 0


Query: 6   VYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQTQLSR 65
           V   +AL+  ++ H  +AA     YGSFG  +H+VGRDG+CLD  +  +   Y  T LS+
Sbjct: 7   VLFCIALAFSLATHTAIAA--ASGYGSFGSRTHLVGRDGLCLDTML--LLGYYPPTHLSQ 66

Query: 66  CDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYE---AKAVLGKCSELIRSDKKWKHRS 125
           C+  K +Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS+ IR D KW    
Sbjct: 67  CEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKWTKNK 126

Query: 126 DGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQSMDN 185
           DGT  H+ SG VLTAKP   V +  +   PSQSWEATE   P V NIKWL+N CL+S++ 
Sbjct: 127 DGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLKSIEG 186

Query: 186 GAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLT-SQRNPSSVILVSNCDDKAT 245
              +V LN C + +KNQ WALY DGTIR + Y  R  CLT S+RN  ++++VS C DK  
Sbjct: 187 ENSYVTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGAMVVVSRCADKPQ 246

Query: 246 QRWDINEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 289
           QRW + ED+ I HPNTNLV+DV R+ P    ++V+D  DG  +Q+WTI
Sbjct: 247 QRWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 288

[Close]

BLAST of MS012496 vs. ExPASy TrEMBL
Match: A0A6J1IYQ6 (ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481109 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 3.1e-66
Identity = 137/293 (46.76%), Postives = 183/293 (62.46%), Query Frame = 0


Query: 1   MRGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQ 60
           MR +++ I +A S+ ++            YG+FG  +H+VGRDG+CLD  +  +   Y Q
Sbjct: 4   MRQVLLCIALAFSLAIAAP--------SEYGAFGGRTHLVGRDGLCLDTML--LSGYYPQ 63

Query: 61  TQLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVP---YNDAYEAKAVLGKCSELIRSDKK 120
           T LS+C   K +Q+W+ILEDGTIR  D  YCLVP   +        V+  C++ +R D K
Sbjct: 64  TDLSQCQPRKLSQLWTILEDGTIRASDVRYCLVPKTQFIGPVGPNVVVMDCTKEVRHDMK 123

Query: 121 WKHRSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCL 180
           W  + DGT  H+ SG VLTAKP   V L  +   PSQSWEATE L P V NIKWL+N CL
Sbjct: 124 WTKKKDGTIHHDESGLVLTAKPGRMVTLEANEDAPSQSWEATESLYPMVANIKWLDNLCL 183

Query: 181 QSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLT-SQRNPSSVILVSNC 240
           +S +    +V LN C + +KNQ WALY DGTIR + Y  R  CLT S+RN  ++++VS C
Sbjct: 184 KSREGENSYVTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGALVVVSRC 243

Query: 241 DDKATQRWDINEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 289
            DK  QRW + EDN I HPNTNLV+DV R+ P    ++V+D  DG P+Q+W+I
Sbjct: 244 ADKPQQRWGLAEDNTINHPNTNLVMDVHREVPLLPPMIVVDKHDGAPSQRWSI 284

[Close]

BLAST of MS012496 vs. ExPASy TrEMBL
Match: A0A6J1FU34 (nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 9.0e-66
Identity = 140/292 (47.95%), Postives = 185/292 (63.36%), Query Frame = 0


Query: 2   RGMVVYIVVALSVLMSVHRGMAAYPYPRYGSFGKISHIVGRDGMCLDIAVPNVRPLYAQT 61
           R +++ +VVA S  ++ H G A      YG+FG  +H+VGRDG+CLD  +  +   Y  T
Sbjct: 5   RQVLLCMVVAFS--LATHIGAAL----GYGAFGGRTHLVGRDGLCLDTML--LLGYYPPT 64

Query: 62  QLSRCDVAKQTQMWSILEDGTIRTKDNVYCLVPYNDAYE---AKAVLGKCSELIRSDKKW 121
            LS+C+  K +Q+WSILEDGTIR  D  +CLVP + + E   A  V   CS+ IR D KW
Sbjct: 65  HLSQCEQRKSSQLWSILEDGTIRISDVRFCLVPKSQSTEFVGANVVAMDCSKEIRHDMKW 124

Query: 122 KHRSDGTFVHENSGFVLTAKPDNFVILGTDAYTPSQSWEATEGLSPSVVNIKWLENQCLQ 181
               DGT  H+ SG VLTAKP   V +  +   PSQSWEATE   P V NIKWL+N CL+
Sbjct: 125 TKNKDGTIHHDESGLVLTAKPGRTVTVEVNENLPSQSWEATETFIPMVANIKWLDNLCLK 184

Query: 182 SMDNGAPFVYLNLCDKKNKNQHWALYSDGTIRVDNYGNRDYCLT-SQRNPSSVILVSNCD 241
           S++    +V LN C + +KNQ WALY DGTIR + Y  R  CLT S+RN  ++++VS C 
Sbjct: 185 SIEGENSYVTLNECSRDDKNQRWALYGDGTIRQNEY--RHLCLTSSERNYGAMVVVSRCA 244

Query: 242 DKATQRWDINEDNFIYHPNTNLVLDVDRK-PEGSQIVVIDTRDGTPTQQWTI 289
           DK  QRW + ED+ I HPNTNLV+DV R+ P    ++V+D  DG  +Q+WTI
Sbjct: 245 DKPQQRWGLAEDSTINHPNTNLVMDVRREVPLLPPMIVVDKHDGAASQRWTI 286

The following BLAST results are available for this feature:

BLAST of MS012496 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149160.1	2.9e-167	98.27	nigrin b-like [Momordica charantia]	[more]
XP_022149159.1	2.5e-142	84.83	beta-galactoside-specific lectin 3-like [Momordica charantia]	[more]
XP_023551012.1	7.6e-67	48.61	nigrin b-like [Cucurbita pepo subsp. pepo]	[more]
XP_022941584.1	1.3e-66	48.26	nigrin b-like isoform X1 [Cucurbita moschata]	[more]
XP_022982216.1	6.4e-66	46.76	ribosome-inactivating protein SNAI-like [Cucurbita maxima]	[more]

BLAST of MS012496 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82683	2.7e-27	31.91	Beta-galactoside-specific lectin 3 OS=Viscum album OX=3972 PE=1 SV=2	[more]
P28590	2.3e-26	28.91	Abrin-c OS=Abrus precatorius OX=3816 PE=1 SV=1	[more]
Q06077	3.9e-26	28.52	Abrin-b OS=Abrus precatorius OX=3816 PE=1 SV=1	[more]
P11140	5.2e-26	29.18	Abrin-a OS=Abrus precatorius OX=3816 PE=1 SV=2	[more]
U3KRF8	6.7e-26	30.53	Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2	[more]

BLAST of MS012496 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4Y7	1.4e-167	98.27	nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111017650 PE=3 SV=1	[more]
A0A6J1D675	1.2e-142	84.83	beta-galactoside-specific lectin 3-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FNV0	6.2e-67	48.26	nigrin b-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=3 SV=1	[more]
A0A6J1IYQ6	3.1e-66	46.76	ribosome-inactivating protein SNAI-like OS=Cucurbita maxima OX=3661 GN=LOC111481...	[more]
A0A6J1FU34	9.0e-66	47.95	nigrin b-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446896 PE=4 SV=1	[more]

BLAST of MS012496 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 163..289 e-value: 3.2E-21 score: 86.5 coord: 33..159 e-value: 1.7E-8 score: 44.2
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 38..156 e-value: 3.9E-15 score: 56.2 coord: 173..286 e-value: 1.1E-20 score: 74.2
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 163..289 score: 28.675499
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 32..159 score: 21.637501
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 38..157 e-value: 1.40671E-13 score: 64.0638
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 167..288 e-value: 2.81468E-22 score: 87.561
None	No IPR available	GENE3D	2.80.10.50		coord: 164..289 e-value: 3.1E-33 score: 116.1
None	No IPR available	GENE3D	2.80.10.50		coord: 35..159 e-value: 2.3E-23 score: 84.4
None	No IPR available	PANTHER	PTHR33453:SF23	RRNA N-GLYCOSIDASE	coord: 38..288
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 38..288
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 167..288
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 25..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012496.1	MS012496.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity