MS012495 (gene) Bitter gourd (TR) v1

Overview


Name	MS012495
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	nigrin b-like
Location	scaffold63: 1096372 .. 1096617 (-)
RNA-Seq Expression	MS012495
Synteny	MS012495

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAATACCACATTCCAGAATTGGGAAGCCACAGAGTGTTTGCAAGTGTTTGCAGTGTCTATAGACGGGGTTCTCTATGTGTATTTGAATGAGTGTAGTAAAGAAATTAAGAACCAAAATTGGGCACTATATCCCGATGACACTATTTGTGTAGATGTCAAAAGACAATATTACTCGACATCTCAAGGAAATCCATCTTCTATCATCCTTGTGTCATACTGCGAAGACAAGCCGACACAGCGATGG

mRNA sequence

AAAAATACCACATTCCAGAATTGGGAAGCCACAGAGTGTTTGCAAGTGTTTGCAGTGTCTATAGACGGGGTTCTCTATGTGTATTTGAATGAGTGTAGTAAAGAAATTAAGAACCAAAATTGGGCACTATATCCCGATGACACTATTTGTGTAGATGTCAAAAGACAATATTACTCGACATCTCAAGGAAATCCATCTTCTATCATCCTTGTGTCATACTGCGAAGACAAGCCGACACAGCGATGG

Coding sequence (CDS)

AAAAATACCACATTCCAGAATTGGGAAGCCACAGAGTGTTTGCAAGTGTTTGCAGTGTCTATAGACGGGGTTCTCTATGTGTATTTGAATGAGTGTAGTAAAGAAATTAAGAACCAAAATTGGGCACTATATCCCGATGACACTATTTGTGTAGATGTCAAAAGACAATATTACTCGACATCTCAAGGAAATCCATCTTCTATCATCCTTGTGTCATACTGCGAAGACAAGCCGACACAGCGATGG

Protein sequence

KNTTFQNWEATECLQVFAVSIDGVLYVYLNECSKEIKNQNWALYPDDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW

Homology

[Close]

BLAST of MS012495 vs. NCBI nr
Match: XP_022149159.1 (beta-galactoside-specific lectin 3-like [Momordica charantia])

HSP 1 Score: 89.7 bits (221), Expect = 1.2e-14
Identity = 49/96 (51.04%), Postives = 58/96 (60.42%), Query Frame = 0


Query: 2   NTTFQNWEATECLQVFAVSI------------DGVLYVYLNECSKEIKNQNWALYPDDTI 61
           NT  Q+WEATE L    V+I            +G  +VYLN C K+ KNQ+WALY D TI
Sbjct: 154 NTPSQSWEATEGLTPSVVNIQWREGLCLQSMDNGAPFVYLNSCDKKNKNQHWALYSDGTI 213

Query: 62  CVD---VKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
            VD    +  Y  TSQ NPSS+ILVS C+DK TQRW
Sbjct: 214 RVDNYGKRSDYCLTSQRNPSSVILVSNCDDKATQRW 249

[Close]

BLAST of MS012495 vs. NCBI nr
Match: XP_022149160.1 (nigrin b-like [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 3.6e-14
Identity = 49/94 (52.13%), Postives = 57/94 (60.64%), Query Frame = 0


Query: 3   TTFQNWEATECLQVFAVSI------------DGVLYVYLNECSKEIKNQNWALYPDDTIC 62
           T  Q+WEATE L    V+I            +G  +VYLN C K+ KNQ+WALY D TI 
Sbjct: 155 TPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIR 214

Query: 63  VD--VKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           VD    R Y  TSQ NPSS+ILVS C+DK TQRW
Sbjct: 215 VDNYGSRDYCLTSQRNPSSVILVSNCDDKATQRW 248

[Close]

BLAST of MS012495 vs. NCBI nr
Match: KAA0039058.1 (nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 73.6 bits (179), Expect = 9.2e-10
Identity = 43/97 (44.33%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 1   KNTTFQNWEATE---------------CLQVFAVSIDGVLYVYLNECSKEIKNQNWALYP 60
           KNT  Q+WEATE               CLQ    S D   +V LN C++E K+Q WALY 
Sbjct: 152 KNTPSQSWEATESSTPMIANIEWLNNLCLQ----STDDSNHVGLNGCNRENKDQRWALYG 211

Query: 61  DDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           D TI  +V R Y  TS       I++S CEDKP QRW
Sbjct: 212 DGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDKPQQRW 244

[Close]

BLAST of MS012495 vs. NCBI nr
Match: TYJ99749.1 (nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 73.6 bits (179), Expect = 9.2e-10
Identity = 43/97 (44.33%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 1   KNTTFQNWEATE---------------CLQVFAVSIDGVLYVYLNECSKEIKNQNWALYP 60
           KNT  Q+WEATE               CLQ    S D   +V LN C++E K+Q WALY 
Sbjct: 152 KNTPSQSWEATESSTPMIANIEWLNNLCLQ----STDDSNHVGLNGCNRENKDQRWALYG 211

Query: 61  DDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           D TI  +V R Y  TS       I++S CEDKP QRW
Sbjct: 212 DGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDKPQQRW 244

[Close]

BLAST of MS012495 vs. NCBI nr
Match: XP_016901886.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 73.6 bits (179), Expect = 9.2e-10
Identity = 43/97 (44.33%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 1   KNTTFQNWEATE---------------CLQVFAVSIDGVLYVYLNECSKEIKNQNWALYP 60
           KNT  Q+WEATE               CLQ    S D   +V LN C++E K+Q WALY 
Sbjct: 152 KNTPSQSWEATESSTPMIANIEWLNNLCLQ----STDDSNHVGLNGCNRENKDQRWALYG 211

Query: 61  DDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           D TI  +V R Y  TS       I++S CEDKP QRW
Sbjct: 212 DGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDKPQQRW 244

[Close]

BLAST of MS012495 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 9.2e-05
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 0


Query: 26  YVYLNECSKEIKNQNWALYPDDTICVDVKRQYYSTSQGN-PSSIILVSYCEDKPTQRW 83
           +V+L +C      Q WALY D TI V+  R    TS+ + PS +I++  CE    QRW
Sbjct: 464 FVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRW 521

[Close]

BLAST of MS012495 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 9.2e-05
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 0


Query: 26  YVYLNECSKEIKNQNWALYPDDTICVDVKRQYYSTSQGN-PSSIILVSYCEDKPTQRW 83
           +V+L +C      Q WALY D TI V+  R    TS+ + PS +I++  CE    QRW
Sbjct: 464 FVWLEDCVLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRW 521

[Close]

BLAST of MS012495 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 46.6 bits (109), Expect = 1.6e-04
Identity = 27/89 (30.34%), Postives = 39/89 (43.82%), Query Frame = 0


Query: 6   QNWEATECLQVFAVSIDGVLY-----------VYLNECSKEIKNQNWALYPDDTICVDVK 65
           Q W  T  ++    SI G              V++ +C      Q WALY D TI V+  
Sbjct: 426 QGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRTIRVNST 485

Query: 66  RQYYSTSQG-NPSSIILVSYCEDKPTQRW 83
           R    T+ G N   +I++  C+  P+QRW
Sbjct: 486 RGLCVTTNGYNSKDLIIILKCQGLPSQRW 514

[Close]

BLAST of MS012495 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 44.3 bits (103), Expect = 7.8e-04
Identity = 22/64 (34.38%), Postives = 32/64 (50.00%), Query Frame = 0


Query: 22  DGVLYVYLNECSKEIKNQNWALYPDDTICVDVKRQYYSTSQGNPS---SIILVSYCEDKP 81
           DG   ++L EC    + Q+WALY D TI VD  R+   T+  +      +I +  C+   
Sbjct: 409 DGNTNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSN 468

Query: 82  TQRW 83
            QRW
Sbjct: 469 NQRW 472

[Close]

BLAST of MS012495 vs. ExPASy TrEMBL
Match: A0A6J1D675 (beta-galactoside-specific lectin 3-like OS=Momordica charantia OX=3673 GN=LOC111017649 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.0e-15
Identity = 49/96 (51.04%), Postives = 58/96 (60.42%), Query Frame = 0


Query: 2   NTTFQNWEATECLQVFAVSI------------DGVLYVYLNECSKEIKNQNWALYPDDTI 61
           NT  Q+WEATE L    V+I            +G  +VYLN C K+ KNQ+WALY D TI
Sbjct: 154 NTPSQSWEATEGLTPSVVNIQWREGLCLQSMDNGAPFVYLNSCDKKNKNQHWALYSDGTI 213

Query: 62  CVD---VKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
            VD    +  Y  TSQ NPSS+ILVS C+DK TQRW
Sbjct: 214 RVDNYGKRSDYCLTSQRNPSSVILVSNCDDKATQRW 249

[Close]

BLAST of MS012495 vs. ExPASy TrEMBL
Match: A0A6J1D4Y7 (nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111017650 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.7e-14
Identity = 49/94 (52.13%), Postives = 57/94 (60.64%), Query Frame = 0


Query: 3   TTFQNWEATECLQVFAVSI------------DGVLYVYLNECSKEIKNQNWALYPDDTIC 62
           T  Q+WEATE L    V+I            +G  +VYLN C K+ KNQ+WALY D TI 
Sbjct: 155 TPSQSWEATEGLSPSVVNIKWLENQCLQSMDNGAPFVYLNLCDKKNKNQHWALYSDGTIR 214

Query: 63  VD--VKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           VD    R Y  TSQ NPSS+ILVS C+DK TQRW
Sbjct: 215 VDNYGSRDYCLTSQRNPSSVILVSNCDDKATQRW 248

[Close]

BLAST of MS012495 vs. ExPASy TrEMBL
Match: A0A5A7T854 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.4e-10
Identity = 43/97 (44.33%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 1   KNTTFQNWEATE---------------CLQVFAVSIDGVLYVYLNECSKEIKNQNWALYP 60
           KNT  Q+WEATE               CLQ    S D   +V LN C++E K+Q WALY 
Sbjct: 152 KNTPSQSWEATESSTPMIANIEWLNNLCLQ----STDDSNHVGLNGCNRENKDQRWALYG 211

Query: 61  DDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           D TI  +V R Y  TS       I++S CEDKP QRW
Sbjct: 212 DGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDKPQQRW 244

[Close]

BLAST of MS012495 vs. ExPASy TrEMBL
Match: A0A5D3BL95 (Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.4e-10
Identity = 43/97 (44.33%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 1   KNTTFQNWEATE---------------CLQVFAVSIDGVLYVYLNECSKEIKNQNWALYP 60
           KNT  Q+WEATE               CLQ    S D   +V LN C++E K+Q WALY 
Sbjct: 152 KNTPSQSWEATESSTPMIANIEWLNNLCLQ----STDDSNHVGLNGCNRENKDQRWALYG 211

Query: 61  DDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           D TI  +V R Y  TS       I++S CEDKP QRW
Sbjct: 212 DGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDKPQQRW 244

[Close]

BLAST of MS012495 vs. ExPASy TrEMBL
Match: A0A1S4E0X9 (nigrin b-like OS=Cucumis melo OX=3656 GN=LOC103496121 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.4e-10
Identity = 43/97 (44.33%), Postives = 51/97 (52.58%), Query Frame = 0


Query: 1   KNTTFQNWEATE---------------CLQVFAVSIDGVLYVYLNECSKEIKNQNWALYP 60
           KNT  Q+WEATE               CLQ    S D   +V LN C++E K+Q WALY 
Sbjct: 152 KNTPSQSWEATESSTPMIANIEWLNNLCLQ----STDDSNHVGLNGCNRENKDQRWALYG 211

Query: 61  DDTICVDVKRQYYSTSQGNPSSIILVSYCEDKPTQRW 83
           D TI  +V R Y  TS       I++S CEDKP QRW
Sbjct: 212 DGTIRQNVNRNYCLTSDEYLGRFIVMSKCEDKPQQRW 244

The following BLAST results are available for this feature:

BLAST of MS012495 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149159.1	1.2e-14	51.04	beta-galactoside-specific lectin 3-like [Momordica charantia]	[more]
XP_022149160.1	3.6e-14	52.13	nigrin b-like [Momordica charantia]	[more]
KAA0039058.1	9.2e-10	44.33	nigrin b-like [Cucumis melo var. makuwa]	[more]
TYJ99749.1	9.2e-10	44.33	nigrin b-like [Cucumis melo var. makuwa]	[more]
XP_016901886.1	9.2e-10	44.33	PREDICTED: nigrin b-like [Cucumis melo]	[more]

BLAST of MS012495 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41358	9.2e-05	39.66	Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1	[more]
O22415	9.2e-05	39.66	Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=...	[more]
P33183	1.6e-04	30.34	Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2	[more]
U3KRF8	7.8e-04	34.38	Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2	[more]

BLAST of MS012495 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D675	6.0e-15	51.04	beta-galactoside-specific lectin 3-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1D4Y7	1.7e-14	52.13	nigrin b-like OS=Momordica charantia OX=3673 GN=LOC111017650 PE=3 SV=1	[more]
A0A5A7T854	4.4e-10	44.33	Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001540 ...	[more]
A0A5D3BL95	4.4e-10	44.33	Nigrin b-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00260 ...	[more]
A0A1S4E0X9	4.4e-10	44.33	nigrin b-like OS=Cucumis melo OX=3656 GN=LOC103496121 PE=4 SV=1	[more]

BLAST of MS012495 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.80.10.50		coord: 3..82 e-value: 9.6E-12 score: 46.7
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 13..82 score: 12.1005
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 8..82

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012495.1	MS012495.1	mRNA