MS012430 (gene) Bitter gourd (TR) v1

Overview


Name	MS012430
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	PUM-HD domain-containing protein
Location	scaffold63: 630731 .. 631297 (-)
RNA-Seq Expression	MS012430
Synteny	MS012430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTTTTCAACGCAATGGCAAGTAATTGTGTAGCAATCGGCACCGATCGAGTGGGTTGTTATGTTCTACAGGATTGTATCTGCACAGCCTATATAGAAGCCAAGCTTGTACCATATTTACTCCACAGCATCGTATTAAACTCACCCTATTTATCAGAAAACTTACACGGGTCTGTTGTTCTTCCACCCAAAAAACACCAAACCAAATTAAACAAATTCTTTATTTATATGTGTGGTTATTTTTTGTGTAGGAACTATGTGGTTCAGTGTGCAATTTCGTCGGGCATTGCTTGTGTAAAACGAGGCATTGCGGAGCGGCTGCAGGGGAGATTCAAAGATCTTTCGAAGAGTATGGGCGGTAGCAATGTGGTGGAGAAGTGCTTGAGGTTGTGTGAAGATTCTGAAGTTGAGAAGATTGTTGAGGAACTGTTGATGAACCCAACAGTTGTTCCCAATGTTGTTCAAGATGCTTTTGGCAACTTTGTTATTCAAACTGCTCTTGAACAGCTCCACCAGCGGGTAAGTGATTTTGTTATATATATATTAACTTCTCTCTGTTTCTTGAAT

mRNA sequence

CTTCTTTTCAACGCAATGGCAAGTAATTGTGTAGCAATCGGCACCGATCGAGTGGGTTGTTATGTTCTACAGGATTGTATCTGCACAGCCTATATAGAAGCCAAGCTTGTACCATATTTACTCCACAGCATCGTATTAAACTCACCCTATTTATCAGAAAACTTACACGGGTCTGTTGTTCTTCCACCCAAAAAACACCAAACCAAATTAAACAAATTCTTTATTTATATGTGTGGTTATTTTTTGTGTAGGAACTATGTGGTTCAGTGTGCAATTTCGTCGGGCATTGCTTGTGTAAAACGAGGCATTGCGGAGCGGCTGCAGGGGAGATTCAAAGATCTTTCGAAGAGTATGGGCGGTAGCAATGTGGTGGAGAAGTGCTTGAGGTTGTGTGAAGATTCTGAAGTTGAGAAGATTGTTGAGGAACTGTTGATGAACCCAACAGTTGTTCCCAATGTTGTTCAAGATGCTTTTGGCAACTTTGTTATTCAAACTGCTCTTGAACAGCTCCACCAGCGGGTAAGTGATTTTGTTATATATATATTAACTTCTCTCTGTTTCTTGAAT

Coding sequence (CDS)

CTTCTTTTCAACGCAATGGCAAGTAATTGTGTAGCAATCGGCACCGATCGAGTGGGTTGTTATGTTCTACAGGATTGTATCTGCACAGCCTATATAGAAGCCAAGCTTGTACCATATTTACTCCACAGCATCGTATTAAACTCACCCTATTTATCAGAAAACTTACACGGGTCTGTTGTTCTTCCACCCAAAAAACACCAAACCAAATTAAACAAATTCTTTATTTATATGTGTGGTTATTTTTTGTGTAGGAACTATGTGGTTCAGTGTGCAATTTCGTCGGGCATTGCTTGTGTAAAACGAGGCATTGCGGAGCGGCTGCAGGGGAGATTCAAAGATCTTTCGAAGAGTATGGGCGGTAGCAATGTGGTGGAGAAGTGCTTGAGGTTGTGTGAAGATTCTGAAGTTGAGAAGATTGTTGAGGAACTGTTGATGAACCCAACAGTTGTTCCCAATGTTGTTCAAGATGCTTTTGGCAACTTTGTTATTCAAACTGCTCTTGAACAGCTCCACCAGCGGGTAAGTGATTTTGTTATATATATATTAACTTCTCTCTGTTTCTTGAAT

Protein sequence

LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVLPPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGSNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLHQRVSDFVIYILTSLCFLN

Homology

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BLAST of MS012430 vs. NCBI nr
Match: XP_022149262.1 (pumilio homolog 12-like [Momordica charantia])

HSP 1 Score: 276.9 bits (707), Expect = 1.3e-70
Identity = 145/173 (83.82%), Postives = 146/173 (84.39%), Query Frame = 0


Query: 1   LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVV 60
           LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHG   
Sbjct: 374 LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHG--- 433

Query: 61  LPPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG 120
                                   NYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG
Sbjct: 434 ------------------------NYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG 493

Query: 121 SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLHQR 174
           SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLH+R
Sbjct: 494 SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLHRR 519

[Close]

BLAST of MS012430 vs. NCBI nr
Match: KAF7128113.1 (hypothetical protein RHSIM_Rhsim11G0156100 [Rhododendron simsii])

HSP 1 Score: 105.1 bits (261), Expect = 6.5e-19
Identity = 64/168 (38.10%), Postives = 92/168 (54.76%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +F  +A NCV IG D+ GC VLQ C+  A  EAK   +L+  I  N+  LSE+ +G    
Sbjct: 399 IFRVVADNCVGIGMDKNGCRVLQKCLDYAQGEAK--EHLMAEIAANALVLSESQYG---- 458

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQC +   I  V  G+ ERL G +  LS S  GS
Sbjct: 459 -----------------------NYVVQCILEMRIPHVTAGVLERLAGNYVSLSMSKYGS 518

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           NVVE+C++L  + +V +I+ E++++P  +  V+QD FGN+V Q+ALE+
Sbjct: 519 NVVERCIKLLGEDQVAQIISEMIVDPNFL-GVLQDPFGNYVAQSALEK 536

[Close]

BLAST of MS012430 vs. NCBI nr
Match: KAE9447116.1 (hypothetical protein C3L33_20973, partial [Rhododendron williamsianum])

HSP 1 Score: 101.7 bits (252), Expect = 7.2e-18
Identity = 63/168 (37.50%), Postives = 91/168 (54.17%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +F  +A NCV IG D+ GC VLQ C+  A  EAK   +L+  I  N+  LSE+ +G    
Sbjct: 400 IFRVVADNCVGIGMDKNGCRVLQKCLDYAQGEAK--EHLMAEIAANALVLSESQYG---- 459

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQC +   I  V  G++ERL G +  LS +  GS
Sbjct: 460 -----------------------NYVVQCILEMRIPHVTAGVSERLAGSYVSLSMNKYGS 519

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           NVVE+C++L  +  V +I+ E++ +P  +  V+QD FGN+V Q+ALE+
Sbjct: 520 NVVERCIKLSGEDLVAQIISEMIADPNFL-GVLQDPFGNYVAQSALEK 537

[Close]

BLAST of MS012430 vs. NCBI nr
Match: KAF7126360.1 (hypothetical protein RHSIM_Rhsim11G0157500 [Rhododendron simsii])

HSP 1 Score: 101.7 bits (252), Expect = 7.2e-18
Identity = 62/168 (36.90%), Postives = 90/168 (53.57%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +F  +A NCV IG D+ GC VLQ C+  A  EAK   +L+  I  N+  LSE+ +G    
Sbjct: 399 IFRVVADNCVGIGMDKNGCRVLQKCLDYAQGEAK--EHLMAEIAANALVLSESQYG---- 458

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQC +   I     G+ ERL G +  LS +  GS
Sbjct: 459 -----------------------NYVVQCILEMRIPHATAGVLERLAGNYVSLSMNKYGS 518

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           NVVE+C++L  + +V +I+ E++ +P  +  V+QD FGN+V Q+ALE+
Sbjct: 519 NVVERCIKLLGEDQVAQIISEMIADPNFL-GVLQDPFGNYVAQSALEK 536

[Close]

BLAST of MS012430 vs. NCBI nr
Match: KAG5526369.1 (hypothetical protein RHGRI_032599 [Rhododendron griersonianum])

HSP 1 Score: 99.0 bits (245), Expect = 4.7e-17
Identity = 60/168 (35.71%), Postives = 89/168 (52.98%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +F  +A NCV IG D+ GC VLQ C+   Y +     +L+  I  N+  LSE+ +G    
Sbjct: 401 IFRVVADNCVGIGMDKNGCRVLQKCL--DYAQGVAKEHLMAEIAANALVLSESQYG---- 460

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQC +   I  V  G++ERL G +  LS +  GS
Sbjct: 461 -----------------------NYVVQCILEMRIPHVTAGVSERLAGSYVSLSMNKYGS 520

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           NVVE+C++L  +  V +I+ E++ +P  +  V+QD FGN+V Q+ALE+
Sbjct: 521 NVVERCIKLSGEDLVAQIISEMIADPNFL-GVLQDPFGNYVAQSALEK 538

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BLAST of MS012430 vs. ExPASy Swiss-Prot
Match: Q9LVC3 (Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.7e-14
Identity = 60/188 (31.91%), Postives = 91/188 (48.40%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           LF A  ++CV + TDR GC VLQ C+   Y E +   +L+  I  N+  LS++  G    
Sbjct: 422 LFEAAITHCVELATDRHGCCVLQKCL--GYSEGEQKQHLVSEIASNALLLSQDPFG---- 481

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYV+Q      +      I E+L+G + +LS     S
Sbjct: 482 -----------------------NYVLQYVFELQLQWATFEILEQLEGNYTELSMQKCSS 541

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ----LHQRVSDF 181
           NVVEKCL+L +D    +I+ EL+ N   +  V+ D +GN+VIQ AL+Q    +H  + D 
Sbjct: 542 NVVEKCLKLADDKHRARIIRELI-NYGRLDQVMLDPYGNYVIQAALKQSKGNVHALLVDA 579

Query: 182 VIYILTSL 186
           +   ++SL
Sbjct: 602 IKLNISSL 579

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BLAST of MS012430 vs. ExPASy Swiss-Prot
Match: Q1PFN9 (Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.0e-06
Identity = 52/166 (31.33%), Postives = 73/166 (43.98%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +  A    C  I   R GC VLQ CI  +    +    L+  I  NS +LS++  G    
Sbjct: 394 VLEAATKYCAEIAIHRHGCCVLQCCISNSVGLQR--ERLVAEISRNSLHLSQDPFG---- 453

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQ  I   ++ VK  +  R+   + +L+     S
Sbjct: 454 -----------------------NYVVQYLIDQQVSAVKLLVQFRM--HYAELATQKFSS 513

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTAL 168
           +V+EKCLR   +S  E IV ELL  P     ++QD + N+VIQTAL
Sbjct: 514 HVIEKCLRKYPESRAE-IVRELLCVPN-FEYLLQDPYANYVIQTAL 526

[Close]

BLAST of MS012430 vs. ExPASy Swiss-Prot
Match: Q9LDW3 (Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 6.6e-06
Identity = 49/168 (29.17%), Postives = 74/168 (44.05%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +  A    C+ I T R GC VLQ C+  +Y   +    L+  I  NS  L+++  G    
Sbjct: 386 ILEAATKFCIEIATTRHGCCVLQRCV--SYSVGEQHEKLVDEISRNSLLLAQDPFG---- 445

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NY+VQ  I   +  V   +   L+G +  L+    GS
Sbjct: 446 -----------------------NYLVQYIIEKKVGGV--NVLFELRGNYVKLATQKFGS 505

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           +VVEKCLR   +S   +IV E L++      ++QD + N+VIQ AL +
Sbjct: 506 HVVEKCLRYYPESR-SQIVNE-LVSVLNFGYLLQDPYANYVIQCALSK 520

[Close]

BLAST of MS012430 vs. ExPASy Swiss-Prot
Match: P25339 (Pumilio homology domain family member 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PUF4 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 1.1e-05
Identity = 57/194 (29.38%), Postives = 88/194 (45.36%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +F+A++ +C+ I T R GC VLQ C             L H        L + L   V  
Sbjct: 705 IFDAISDSCIDIATHRHGCCVLQRC-------------LDHGTTEQCDNLCDKLLALV-- 764

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVK----RGIAERLQGRFKDLSKS 121
                +  L+ F           NYVVQ  I+      K      I   L+ R  +LS  
Sbjct: 765 ----DKLTLDPF----------GNYVVQYIITKEAEKNKYDYTHKIVHLLKPRAIELSIH 824

Query: 122 MGGSNVVEKCLRLCEDSEVEKIVEELLMN--PTVVPNVVQDAFGNFVIQTAL-------E 181
             GSNV+EK L+    S  E ++ E+L N   T + +++ D++GN+V+QTAL       +
Sbjct: 825 KFGSNVIEKILKTAIVS--EPMILEILNNGGETGIQSLLNDSYGNYVLQTALDISHKQND 867

Query: 182 QLHQRVSDFVIYIL 183
            L++R+S+ V  +L
Sbjct: 885 YLYKRLSEIVAPLL 867

[Close]

BLAST of MS012430 vs. ExPASy Swiss-Prot
Match: Q9LP21 (Putative pumilio homolog 10 OS=Arabidopsis thaliana OX=3702 GN=APUM10 PE=3 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 3.3e-05
Identity = 51/164 (31.10%), Postives = 72/164 (43.90%), Query Frame = 0


Query: 5   AMASNCVAIGTDRVGCYVLQ-DCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVLPP 64
           A    C  + T + GCYVLQ   I T  ++ +    L+  I  +S  LS++  G      
Sbjct: 361 AATEYCAQLATHQYGCYVLQCSLINTVGLQHE---RLVAEISRDSLRLSQDPFG------ 420

Query: 65  KKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGSNV 124
                                NYVVQC I   ++ V   +  R      +L+     S+V
Sbjct: 421 ---------------------NYVVQCLIDQQVSSVNLLLPFRT--HCIELATQKFSSHV 480

Query: 125 VEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTAL 168
           +EKCLR   +S  E IV ELL  P     ++QD + N+VIQTAL
Sbjct: 481 IEKCLRKYPESRAE-IVRELLSYPN-FEQLLQDPYANYVIQTAL 490

[Close]

BLAST of MS012430 vs. ExPASy TrEMBL
Match: A0A6J1D7G3 (pumilio homolog 12-like OS=Momordica charantia OX=3673 GN=LOC111017727 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 6.1e-71
Identity = 145/173 (83.82%), Postives = 146/173 (84.39%), Query Frame = 0


Query: 1   LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVV 60
           LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHG   
Sbjct: 374 LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHG--- 433

Query: 61  LPPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG 120
                                   NYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG
Sbjct: 434 ------------------------NYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG 493

Query: 121 SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLHQR 174
           SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLH+R
Sbjct: 494 SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLHRR 519

[Close]

BLAST of MS012430 vs. ExPASy TrEMBL
Match: A0A6A4KFF9 (PUM-HD domain-containing protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_20973 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 3.5e-18
Identity = 63/168 (37.50%), Postives = 91/168 (54.17%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +F  +A NCV IG D+ GC VLQ C+  A  EAK   +L+  I  N+  LSE+ +G    
Sbjct: 400 IFRVVADNCVGIGMDKNGCRVLQKCLDYAQGEAK--EHLMAEIAANALVLSESQYG---- 459

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQC +   I  V  G++ERL G +  LS +  GS
Sbjct: 460 -----------------------NYVVQCILEMRIPHVTAGVSERLAGSYVSLSMNKYGS 519

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           NVVE+C++L  +  V +I+ E++ +P  +  V+QD FGN+V Q+ALE+
Sbjct: 520 NVVERCIKLSGEDLVAQIISEMIADPNFL-GVLQDPFGNYVAQSALEK 537

[Close]

BLAST of MS012430 vs. ExPASy TrEMBL
Match: A0A2P5CSZ8 (Coatomer beta subunit OS=Parasponia andersonii OX=3476 GN=PanWU01x14_126920 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 6.2e-15
Identity = 62/181 (34.25%), Postives = 88/181 (48.62%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           LF A   NCV + TDR GC VLQ C+C  + + +    L+  I  N+  LS++  G    
Sbjct: 598 LFEAAIMNCVELATDRHGCCVLQKCLC--HSDGERRHRLICEIASNAIILSQDPFG---- 657

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQ     G+      I ++L G + DLS     S
Sbjct: 658 -----------------------NYVVQYVFELGLPWAISNILDQLDGSYGDLSVQKHSS 717

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ----LHQRVSDF 179
           NVVEKCL+   +    +I++EL+ NP  +  ++QD FGN+VIQ ALEQ    LH ++ D 
Sbjct: 718 NVVEKCLKCAGEERRTRIIQELIDNPR-LDQIMQDPFGNYVIQAALEQSKGSLHAKLVDT 748

[Close]

BLAST of MS012430 vs. ExPASy TrEMBL
Match: A0A2Z6NB06 (PUM-HD domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_72120 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.1e-14
Identity = 62/175 (35.43%), Postives = 88/175 (50.29%), Query Frame = 0


Query: 1   LLFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVV 60
           ++ + +A NCV I T++VGC V+Q C+   Y     +  LL  I+ NS  L+ + +G   
Sbjct: 225 IILDEVARNCVDIATNKVGCSVIQKCL--DYSGKSAIHLLLFKIITNSLLLAPSPYG--- 284

Query: 61  LPPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGG 120
                                   NYVVQ  I   +    + I E L+G F  LS +   
Sbjct: 285 ------------------------NYVVQYVIKMEVPLANKVIIEELRGNFVRLSMNKFS 344

Query: 121 SNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALE--QLHQR 174
           SNVVE  LR    S+VE +VEE++ +  ++ NV+QD +GNFV Q ALE  Q HQR
Sbjct: 345 SNVVEDLLRYSNQSDVEVVVEEIIKSLNIL-NVLQDPYGNFVAQRALEYTQGHQR 369

[Close]

BLAST of MS012430 vs. ExPASy TrEMBL
Match: A0A4P1RPA1 (PUM-HD domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_30618 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.1e-14
Identity = 59/181 (32.60%), Postives = 92/181 (50.83%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           L   +A++C  I TD+ GC VL  CI  A+ E K    L+  I++N+P L+E+ +G    
Sbjct: 351 LLRVVANHCFGIATDKTGCCVLHQCISYAHGETK--KQLIAEIIVNAPLLAEDAYG---- 410

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQ  +SS    V   +  +L+G+F  LS++  GS
Sbjct: 411 -----------------------NYVVQDLVSSKTPIVTECLLRQLEGKFLSLSRNKYGS 470

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQLHQRVSDFVIYI 181
           NVVEK L    D    +I+ ELL NP V   ++ D FGN+VI++A+ +    + D ++ +
Sbjct: 471 NVVEKFLVEVGDQHKRRIILELLHNPDVA-RLLVDPFGNYVIKSAVSKSKGAIQDALLQL 501

Query: 182 L 183
           +
Sbjct: 531 I 501

[Close]

BLAST of MS012430 vs. TAIR 10
Match: AT5G56510.1 (pumilio 12 )

HSP 1 Score: 80.9 bits (198), Expect = 1.2e-15
Identity = 60/188 (31.91%), Postives = 91/188 (48.40%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           LF A  ++CV + TDR GC VLQ C+   Y E +   +L+  I  N+  LS++  G    
Sbjct: 422 LFEAAITHCVELATDRHGCCVLQKCL--GYSEGEQKQHLVSEIASNALLLSQDPFG---- 481

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYV+Q      +      I E+L+G + +LS     S
Sbjct: 482 -----------------------NYVLQYVFELQLQWATFEILEQLEGNYTELSMQKCSS 541

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ----LHQRVSDF 181
           NVVEKCL+L +D    +I+ EL+ N   +  V+ D +GN+VIQ AL+Q    +H  + D 
Sbjct: 542 NVVEKCLKLADDKHRARIIRELI-NYGRLDQVMLDPYGNYVIQAALKQSKGNVHALLVDA 579

Query: 182 VIYILTSL 186
           +   ++SL
Sbjct: 602 IKLNISSL 579

[Close]

BLAST of MS012430 vs. TAIR 10
Match: AT1G35730.1 (pumilio 9 )

HSP 1 Score: 55.1 bits (131), Expect = 7.2e-08
Identity = 52/166 (31.33%), Postives = 73/166 (43.98%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +  A    C  I   R GC VLQ CI  +    +    L+  I  NS +LS++  G    
Sbjct: 394 VLEAATKYCAEIAIHRHGCCVLQCCISNSVGLQR--ERLVAEISRNSLHLSQDPFG---- 453

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NYVVQ  I   ++ VK  +  R+   + +L+     S
Sbjct: 454 -----------------------NYVVQYLIDQQVSAVKLLVQFRM--HYAELATQKFSS 513

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTAL 168
           +V+EKCLR   +S  E IV ELL  P     ++QD + N+VIQTAL
Sbjct: 514 HVIEKCLRKYPESRAE-IVRELLCVPN-FEYLLQDPYANYVIQTAL 526

[Close]

BLAST of MS012430 vs. TAIR 10
Match: AT4G08840.1 (pumilio 11 )

HSP 1 Score: 52.4 bits (124), Expect = 4.7e-07
Identity = 49/168 (29.17%), Postives = 74/168 (44.05%), Query Frame = 0


Query: 2   LFNAMASNCVAIGTDRVGCYVLQDCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVL 61
           +  A    C+ I T R GC VLQ C+  +Y   +    L+  I  NS  L+++  G    
Sbjct: 386 ILEAATKFCIEIATTRHGCCVLQRCV--SYSVGEQHEKLVDEISRNSLLLAQDPFG---- 445

Query: 62  PPKKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGS 121
                                  NY+VQ  I   +  V   +   L+G +  L+    GS
Sbjct: 446 -----------------------NYLVQYIIEKKVGGV--NVLFELRGNYVKLATQKFGS 505

Query: 122 NVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTALEQ 170
           +VVEKCLR   +S   +IV E L++      ++QD + N+VIQ AL +
Sbjct: 506 HVVEKCLRYYPESR-SQIVNE-LVSVLNFGYLLQDPYANYVIQCALSK 520

[Close]

BLAST of MS012430 vs. TAIR 10
Match: AT5G09610.1 (pumilio 21 )

HSP 1 Score: 50.8 bits (120), Expect = 1.4e-06
Identity = 29/71 (40.85%), Postives = 42/71 (59.15%), Query Frame = 0


Query: 103 IAERLQGRFKDLSKSMGGSNVVEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFV 162
           IAERL+G F  L+    GS VVEKCL+   D   ++++EE   N      +  D FGNFV
Sbjct: 398 IAERLRGSFFRLAMERQGSYVVEKCLK--SDFARDQVLEEFRGNAKEWVRMTTDKFGNFV 457

Query: 163 IQTALEQLHQR 174
           +Q+AL  + ++
Sbjct: 458 VQSALRVMKEK 466

[Close]

BLAST of MS012430 vs. TAIR 10
Match: AT1G35750.1 (pumilio 10 )

HSP 1 Score: 50.1 bits (118), Expect = 2.3e-06
Identity = 51/164 (31.10%), Postives = 72/164 (43.90%), Query Frame = 0


Query: 5   AMASNCVAIGTDRVGCYVLQ-DCICTAYIEAKLVPYLLHSIVLNSPYLSENLHGSVVLPP 64
           A    C  + T + GCYVLQ   I T  ++ +    L+  I  +S  LS++  G      
Sbjct: 361 AATEYCAQLATHQYGCYVLQCSLINTVGLQHE---RLVAEISRDSLRLSQDPFG------ 420

Query: 65  KKHQTKLNKFFIYMCGYFLCRNYVVQCAISSGIACVKRGIAERLQGRFKDLSKSMGGSNV 124
                                NYVVQC I   ++ V   +  R      +L+     S+V
Sbjct: 421 ---------------------NYVVQCLIDQQVSSVNLLLPFRT--HCIELATQKFSSHV 480

Query: 125 VEKCLRLCEDSEVEKIVEELLMNPTVVPNVVQDAFGNFVIQTAL 168
           +EKCLR   +S  E IV ELL  P     ++QD + N+VIQTAL
Sbjct: 481 IEKCLRKYPESRAE-IVRELLSYPN-FEQLLQDPYANYVIQTAL 490

The following BLAST results are available for this feature:

BLAST of MS012430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149262.1	1.3e-70	83.82	pumilio homolog 12-like [Momordica charantia]	[more]
KAF7128113.1	6.5e-19	38.10	hypothetical protein RHSIM_Rhsim11G0156100 [Rhododendron simsii]	[more]
KAE9447116.1	7.2e-18	37.50	hypothetical protein C3L33_20973, partial [Rhododendron williamsianum]	[more]
KAF7126360.1	7.2e-18	36.90	hypothetical protein RHSIM_Rhsim11G0157500 [Rhododendron simsii]	[more]
KAG5526369.1	4.7e-17	35.71	hypothetical protein RHGRI_032599 [Rhododendron griersonianum]	[more]

BLAST of MS012430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVC3	1.7e-14	31.91	Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2	[more]
Q1PFN9	1.0e-06	31.33	Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1	[more]
Q9LDW3	6.6e-06	29.17	Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2	[more]
P25339	1.1e-05	29.38	Pumilio homology domain family member 4 OS=Saccharomyces cerevisiae (strain ATCC...	[more]
Q9LP21	3.3e-05	31.10	Putative pumilio homolog 10 OS=Arabidopsis thaliana OX=3702 GN=APUM10 PE=3 SV=2	[more]

BLAST of MS012430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7G3	6.1e-71	83.82	pumilio homolog 12-like OS=Momordica charantia OX=3673 GN=LOC111017727 PE=4 SV=1	[more]
A0A6A4KFF9	3.5e-18	37.50	PUM-HD domain-containing protein (Fragment) OS=Rhododendron williamsianum OX=262...	[more]
A0A2P5CSZ8	6.2e-15	34.25	Coatomer beta subunit OS=Parasponia andersonii OX=3476 GN=PanWU01x14_126920 PE=4...	[more]
A0A2Z6NB06	3.1e-14	35.43	PUM-HD domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_72120...	[more]
A0A4P1RPA1	3.1e-14	32.60	PUM-HD domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_306...	[more]

BLAST of MS012430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56510.1	1.2e-15	31.91	pumilio 12	[more]
AT1G35730.1	7.2e-08	31.33	pumilio 9	[more]
AT4G08840.1	4.7e-07	29.17	pumilio 11	[more]
AT5G09610.1	1.4e-06	40.85	pumilio 21	[more]
AT1G35750.1	2.3e-06	31.10	pumilio 10	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001313	Pumilio RNA-binding repeat	SMART	SM00025	pum_5	coord: 145..180 e-value: 43.0 score: 8.0 coord: 106..141 e-value: 0.013 score: 24.6 coord: 5..42 e-value: 180.0 score: 3.2
IPR001313	Pumilio RNA-binding repeat	PFAM	PF00806	PUF	coord: 9..27 e-value: 0.002 score: 17.8 coord: 155..169 e-value: 6.7 score: 6.5 coord: 111..144 e-value: 3.5E-6 score: 26.6
IPR001313	Pumilio RNA-binding repeat	PROSITE	PS50302	PUM	coord: 106..141 score: 8.757235
IPR001313	Pumilio RNA-binding repeat	PROSITE	PS50302	PUM	coord: 142..180 score: 7.520671
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 1..182 e-value: 1.1E-30 score: 109.0
None	No IPR available	PANTHER	PTHR12537	RNA BINDING PROTEIN PUMILIO-RELATED	coord: 85..174 coord: 4..60
None	No IPR available	PANTHER	PTHR12537:SF129	PUMILIO-LIKE PROTEIN	coord: 85..174 coord: 4..60
IPR033133	Pumilio homology domain	PROSITE	PS50303	PUM_HD	coord: 1..189 score: 16.382437
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 2..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012430.1	MS012430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003723	RNA binding