MS012408 (gene) Bitter gourd (TR) v1

Overview


Name	MS012408
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Trypsin inhibitor 1
Location	scaffold63: 499038 .. 499307 (-)
RNA-Seq Expression	MS012408
Synteny	MS012408

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAAGAAGATTTTCATTGTGGTGGCGCTGGTGGCGATGATGCTTGTTGCGTCGTCGGCCACCTTCGAGGAGGGCGACATGAGACCTCTCGTCTCCGACGATGGAGCTGTAGCTGGTCAAGGCATGAATGATTATCCAAGAAAAATGTTTGTGAAAGTTGTGCATTACGAGAATCAAAGAAGGTGTCCGAGAATCTTGAAGCAATGCAAACGGGACTCGGATTGCCCGGGTGAGTGTATTTGCATGGCGCACGGGTTCTGCGGT

mRNA sequence

ATGGAGAGCAAGAAGATTTTCATTGTGGTGGCGCTGGTGGCGATGATGCTTGTTGCGTCGTCGGCCACCTTCGAGGAGGGCGACATGAGACCTCTCGTCTCCGACGATGGAGCTGTAGCTGGTCAAGGCATGAATGATTATCCAAGAAAAATGTTTGTGAAAGTTGTGCATTACGAGAATCAAAGAAGGTGTCCGAGAATCTTGAAGCAATGCAAACGGGACTCGGATTGCCCGGGTGAGTGTATTTGCATGGCGCACGGGTTCTGCGGT

Coding sequence (CDS)

ATGGAGAGCAAGAAGATTTTCATTGTGGTGGCGCTGGTGGCGATGATGCTTGTTGCGTCGTCGGCCACCTTCGAGGAGGGCGACATGAGACCTCTCGTCTCCGACGATGGAGCTGTAGCTGGTCAAGGCATGAATGATTATCCAAGAAAAATGTTTGTGAAAGTTGTGCATTACGAGAATCAAAGAAGGTGTCCGAGAATCTTGAAGCAATGCAAACGGGACTCGGATTGCCCGGGTGAGTGTATTTGCATGGCGCACGGGTTCTGCGGT

Protein sequence

MESKKIFIVVALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQCKRDSDCPGECICMAHGFCG

Homology

[Close]

BLAST of MS012408 vs. NCBI nr
Match: AIZ03439.1 (TIPRE3 precursor, partial [Momordica anigosantha])

HSP 1 Score: 85.5 bits (210), Expect = 2.6e-13
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 12 LVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQC 71
          LVA MLVA+SA F +GD   L+S+D A  GQ +N          V+ E QR CPRILK+C
Sbjct: 4  LVATMLVATSADFNDGDTIDLISNDRAQTGQDING-------GGVYSEEQRACPRILKRC 63

Query: 72 KRDSDCPGECICMAHGFCG 91
          +RDSDCPG C+C  +G+CG
Sbjct: 64 RRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MS012408 vs. NCBI nr
Match: AIZ03440.1 (TIPRE4 precursor, partial [Momordica anigosantha])

HSP 1 Score: 85.5 bits (210), Expect = 2.6e-13
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 12 LVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQC 71
          LVA MLVA+SA F +GD   L+S+D A  GQ +N          V+ E QR CPRILK+C
Sbjct: 4  LVATMLVATSADFNDGDTIDLISNDRAQTGQDING-------GGVYSEEQRACPRILKRC 63

Query: 72 KRDSDCPGECICMAHGFCG 91
          +RDSDCPG C+C  +G+CG
Sbjct: 64 RRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MS012408 vs. NCBI nr
Match: AIZ03438.1 (TIPRE2 precursor, partial [Momordica anigosantha])

HSP 1 Score: 83.2 bits (204), Expect = 1.3e-12
Identity = 42/80 (52.50%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 11 ALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQ 70
          ALVAMMLVA+SA F  GD   L+S+  A  GQ +N          V+ E QR CP+ILK+
Sbjct: 3  ALVAMMLVATSADFNGGDTIHLISNGRAQTGQDINS-------GGVYSEEQRACPKILKR 62

Query: 71 CKRDSDCPGECICMAHGFCG 91
          C+RDSDCPG C+C  +G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MS012408 vs. NCBI nr
Match: AIZ03437.1 (TIPRE1 precursor, partial [Momordica anigosantha])

HSP 1 Score: 83.2 bits (204), Expect = 1.3e-12
Identity = 42/80 (52.50%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 11 ALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQ 70
          ALVAMMLVA+SA F  GD   L+S+  A  GQ +N          V+ E QR CP+ILK+
Sbjct: 3  ALVAMMLVATSADFNGGDTIHLISNGRAQTGQDINS-------GGVYSEEQRACPKILKR 62

Query: 71 CKRDSDCPGECICMAHGFCG 91
          C+RDSDCPG C+C  +G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MS012408 vs. NCBI nr
Match: AIZ03441.1 (TIPRE5 precursor, partial [Momordica friesiorum])

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-11
Identity = 41/80 (51.25%), Postives = 52/80 (65.00%), Query Frame = 0


Query: 11 ALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQ 70
          ALVAMMLVA+SA    GD   L+S+  A  GQ +N          V+ E QR CP+ILK+
Sbjct: 3  ALVAMMLVATSADSNGGDTIHLISNGRAQTGQDINS-------GGVYSEEQRACPKILKR 62

Query: 71 CKRDSDCPGECICMAHGFCG 91
          C+RDSDCPG C+C  +G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MS012408 vs. ExPASy Swiss-Prot
Match: P10294 (Trypsin inhibitor 1 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.7e-12
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 61 QRRCPRILKQCKRDSDCPGECICMAHGFCG 91
          +RRCPRILKQCKRDSDCPGECICMAHGFCG
Sbjct: 1  ERRCPRILKQCKRDSDCPGECICMAHGFCG 30

[Close]

BLAST of MS012408 vs. ExPASy Swiss-Prot
Match: Q9S747 (Trypsin inhibitor 3 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 3.2e-11
Identity = 27/30 (90.00%), Postives = 29/30 (96.67%), Query Frame = 0


Query: 61 QRRCPRILKQCKRDSDCPGECICMAHGFCG 91
          +R CPRILKQCK+DSDCPGECICMAHGFCG
Sbjct: 1  ERGCPRILKQCKQDSDCPGECICMAHGFCG 30

[Close]

BLAST of MS012408 vs. ExPASy Swiss-Prot
Match: P82410 (Trypsin inhibitor 3 OS=Momordica cochinchinensis OX=3674 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 6.8e-09
Identity = 23/30 (76.67%), Postives = 27/30 (90.00%), Query Frame = 0


Query: 61 QRRCPRILKQCKRDSDCPGECICMAHGFCG 91
          QR CPRILK+C+RDSDCPGECIC  +G+CG
Sbjct: 1  QRACPRILKKCRRDSDCPGECICKENGYCG 30

[Close]

BLAST of MS012408 vs. ExPASy Swiss-Prot
Match: P82409 (Trypsin inhibitor 2 OS=Momordica cochinchinensis OX=3674 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.9e-07
Identity = 19/27 (70.37%), Postives = 24/27 (88.89%), Query Frame = 0


Query: 64 CPRILKQCKRDSDCPGECICMAHGFCG 91
          CP+ILK+C+RDSDCPG CIC  +G+CG
Sbjct: 8  CPKILKKCRRDSDCPGACICRGNGYCG 34

[Close]

BLAST of MS012408 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 8.3e-07
Identity = 19/30 (63.33%), Postives = 25/30 (83.33%), Query Frame = 0


Query: 61 QRRCPRILKQCKRDSDCPGECICMAHGFCG 91
          +R CPRIL +CK+DSDC  EC+C+ HG+CG
Sbjct: 3  ERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of MS012408 vs. ExPASy TrEMBL
Match: A0A0A7HF94 (TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-13
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 12 LVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQC 71
          LVA MLVA+SA F +GD   L+S+D A  GQ +N          V+ E QR CPRILK+C
Sbjct: 4  LVATMLVATSADFNDGDTIDLISNDRAQTGQDING-------GGVYSEEQRACPRILKRC 63

Query: 72 KRDSDCPGECICMAHGFCG 91
          +RDSDCPG C+C  +G+CG
Sbjct: 64 RRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MS012408 vs. ExPASy TrEMBL
Match: A0A0A7HIT2 (TIPRE4 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE4 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-13
Identity = 42/79 (53.16%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 12 LVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQC 71
          LVA MLVA+SA F +GD   L+S+D A  GQ +N          V+ E QR CPRILK+C
Sbjct: 4  LVATMLVATSADFNDGDTIDLISNDRAQTGQDING-------GGVYSEEQRACPRILKRC 63

Query: 72 KRDSDCPGECICMAHGFCG 91
          +RDSDCPG C+C  +G+CG
Sbjct: 64 RRDSDCPGACVCQGNGYCG 75

[Close]

BLAST of MS012408 vs. ExPASy TrEMBL
Match: A0A0A7HG47 (TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.1e-13
Identity = 42/80 (52.50%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 11 ALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQ 70
          ALVAMMLVA+SA F  GD   L+S+  A  GQ +N          V+ E QR CP+ILK+
Sbjct: 3  ALVAMMLVATSADFNGGDTIHLISNGRAQTGQDINS-------GGVYSEEQRACPKILKR 62

Query: 71 CKRDSDCPGECICMAHGFCG 91
          C+RDSDCPG C+C  +G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MS012408 vs. ExPASy TrEMBL
Match: A0A0A7HF33 (TIPRE2 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE2 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.1e-13
Identity = 42/80 (52.50%), Postives = 53/80 (66.25%), Query Frame = 0


Query: 11 ALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQ 70
          ALVAMMLVA+SA F  GD   L+S+  A  GQ +N          V+ E QR CP+ILK+
Sbjct: 3  ALVAMMLVATSADFNGGDTIHLISNGRAQTGQDINS-------GGVYSEEQRACPKILKR 62

Query: 71 CKRDSDCPGECICMAHGFCG 91
          C+RDSDCPG C+C  +G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MS012408 vs. ExPASy TrEMBL
Match: A0A0A7HIA9 (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 5.2e-12
Identity = 41/80 (51.25%), Postives = 52/80 (65.00%), Query Frame = 0


Query: 11 ALVAMMLVASSATFEEGDMRPLVSDDGAVAGQGMNDYPRKMFVKVVHYENQRRCPRILKQ 70
          ALVAMMLVA+SA    GD   L+S+  A  GQ +N          V+ E QR CP+ILK+
Sbjct: 3  ALVAMMLVATSADSNGGDTIHLISNGRAQTGQDINS-------GGVYSEEQRACPKILKR 62

Query: 71 CKRDSDCPGECICMAHGFCG 91
          C+RDSDCPG C+C  +G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

The following BLAST results are available for this feature:

BLAST of MS012408 vs. NCBI nr


Match Name	E-value	Identity	Description
AIZ03439.1	2.6e-13	53.16	TIPRE3 precursor, partial [Momordica anigosantha]	[more]
AIZ03440.1	2.6e-13	53.16	TIPRE4 precursor, partial [Momordica anigosantha]	[more]
AIZ03438.1	1.3e-12	52.50	TIPRE2 precursor, partial [Momordica anigosantha]	[more]
AIZ03437.1	1.3e-12	52.50	TIPRE1 precursor, partial [Momordica anigosantha]	[more]
AIZ03441.1	1.1e-11	51.25	TIPRE5 precursor, partial [Momordica friesiorum]	[more]

BLAST of MS012408 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10294	1.7e-12	96.67	Trypsin inhibitor 1 OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
Q9S747	3.2e-11	90.00	Trypsin inhibitor 3 OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P82410	6.8e-09	76.67	Trypsin inhibitor 3 OS=Momordica cochinchinensis OX=3674 PE=1 SV=1	[more]
P82409	4.9e-07	70.37	Trypsin inhibitor 2 OS=Momordica cochinchinensis OX=3674 PE=1 SV=1	[more]
P07853	8.3e-07	63.33	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]

BLAST of MS012408 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A7HF94	1.2e-13	53.16	TIPRE3 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE3 PE=3 SV=1	[more]
A0A0A7HIT2	1.2e-13	53.16	TIPRE4 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE4 PE=3 SV=1	[more]
A0A0A7HG47	6.1e-13	52.50	TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1	[more]
A0A0A7HF33	6.1e-13	52.50	TIPRE2 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE2 PE=3 SV=1	[more]
A0A0A7HIA9	5.2e-12	51.25	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]

BLAST of MS012408 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 62..90 e-value: 1.4E-12 score: 57.8
None	No IPR available	GENE3D	4.10.75.20		coord: 39..90 e-value: 2.9E-16 score: 61.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 62..90 e-value: 7.8E-16 score: 57.8
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 64..83
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 64..90 e-value: 5.51492E-13 score: 55.4615
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 59..90

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012408.1	MS012408.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity