MS012402 (gene) Bitter gourd (TR) v1

BLAST of MS012402 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 68.6 bits (166), Expect = 2.6e-08
Identity = 38/79 (48.10%), Postives = 52/79 (65.82%), Query Frame = 0

Query: 1   MGWKKFALVAIVGMLLMGASAQA----GG--AETVATEIQGRPRRMMRGGI-CPRILMKC 60
           M WK+ AL+A+VG+LLM A A++    GG   + V+  +   PR+MM G + CPRILM+C
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALGCPRILMEC 142

Query: 61  KKTSDCMAQCKCLSNGFCG 73
           +  SDC+  C C  NGFCG
Sbjct: 143 EVDSDCLPGCICRPNGFCG 161

BLAST of MS012402 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 68.6 bits (166), Expect = 2.6e-08
Identity = 38/79 (48.10%), Postives = 52/79 (65.82%), Query Frame = 0

Query: 1   MGWKKFALVAIVGMLLMGASAQA----GG--AETVATEIQGRPRRMMRGGI-CPRILMKC 60
           M WK+ AL+A+VG+LLM A A++    GG   + V+  +   PR+MM G + CPRILM+C
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALGCPRILMEC 142

Query: 61  KKTSDCMAQCKCLSNGFCG 73
           +  SDC+  C C  NGFCG
Sbjct: 143 EVDSDCLPGCICRPNGFCG 161

BLAST of MS012402 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 66.2 bits (160), Expect = 1.3e-07
Identity = 38/79 (48.10%), Postives = 49/79 (62.03%), Query Frame = 0

Query: 1   MGWKKFALVAIVGMLLMGA---SAQAGGAET---VATEIQGRPRRMMRGGI-CPRILMKC 60
           M WK+ AL+A+VG+LLM A   SA   G E    V+  +   PR+MM   + CPRILM+C
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 144

Query: 61  KKTSDCMAQCKCLSNGFCG 73
           +  SDC+  C C  NGFCG
Sbjct: 145 EVDSDCLPGCVCRPNGFCG 163

BLAST of MS012402 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 65.5 bits (158), Expect = 2.2e-07
Identity = 38/79 (48.10%), Postives = 51/79 (64.56%), Query Frame = 0

Query: 1  MGWKKFALVAIVGMLLMGASAQA----GG--AETVATEIQGRPRRMMRG-GICPRILMKC 60
          M WK+ AL+A+VG+LLM A A++    GG   + V+  +   PR+MM G   CPRILM+C
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILMEC 60

Query: 61 KKTSDCMAQCKCLSNGFCG 73
          +  SDC+  C C  NGFCG
Sbjct: 61 ELDSDCLPGCICRPNGFCG 79

BLAST of MS012402 vs. NCBI nr
Match: KAG6576914.1 (hypothetical protein SDJN03_24488, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 63.5 bits (153), Expect = 8.3e-07
Identity = 35/81 (43.21%), Postives = 48/81 (59.26%), Query Frame = 0

Query: 1  MGWKKFALVAIVGMLLMGASAQAGGAETVATEI--------QGRPRRMMRGG-ICPRILM 60
          M WK+ ALVA+VG+LL+ A A++   +    E+           PR+MM+G   CPR L+
Sbjct: 1  MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60

Query: 61 KCKKTSDCMAQCKCLSNGFCG 73
          +CKK SDC   CKC+   FCG
Sbjct: 61 RCKKDSDCHRACKCMPYHFCG 81

BLAST of MS012402 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.6e-08
Identity = 25/39 (64.10%), Postives = 29/39 (74.36%), Query Frame = 0

Query: 37 PRRMM---RGGICPRILMKCKKTSDCMAQCKCLSNGFCG 73
          PR++M    GGICPRILM CK   DCM  C+CLSNG+CG
Sbjct: 25 PRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG 63

BLAST of MS012402 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 8.6e-07
Identity = 20/28 (71.43%), Postives = 25/28 (89.29%), Query Frame = 0

Query: 45 ICPRILMKCKKTSDCMAQCKCLSNGFCG 73
          +CPRILMKCKK SDC+A+C CL +G+CG
Sbjct: 5  VCPRILMKCKKDSDCLAECVCLEHGYCG 32

BLAST of MS012402 vs. ExPASy Swiss-Prot
Match: P83398 (Trypsin inhibitor 7 OS=Cyclanthera pedata OX=198836 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.9e-06
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 0

Query: 45 ICPRILMKCKKTSDCMAQCKCLSNGFCG 73
          ICPRILMKCKK SDC+A+C C  +GFCG
Sbjct: 3  ICPRILMKCKKDSDCLAECICQEHGFCG 30

BLAST of MS012402 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 19/28 (67.86%), Postives = 25/28 (89.29%), Query Frame = 0

Query: 45 ICPRILMKCKKTSDCMAQCKCLSNGFCG 73
          +CPRILM+CKK SDC+A+C CL +G+CG
Sbjct: 2  VCPRILMECKKDSDCLAECVCLEHGYCG 29

BLAST of MS012402 vs. ExPASy Swiss-Prot
Match: P83397 (Trypsin inhibitor 6 OS=Cyclanthera pedata OX=198836 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 0

Query: 45 ICPRILMKCKKTSDCMAQCKCLSNGFCG 73
          ICPRILMKCKK SDC+A+C C  +GFCG
Sbjct: 3  ICPRILMKCKKDSDCLAECICEEHGFCG 30

BLAST of MS012402 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-07
Identity = 38/79 (48.10%), Postives = 51/79 (64.56%), Query Frame = 0

Query: 1  MGWKKFALVAIVGMLLMGASAQA----GG--AETVATEIQGRPRRMMRG-GICPRILMKC 60
          M WK+ AL+A+VG+LLM A A++    GG   + V+  +   PR+MM G   CPRILM+C
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILMEC 60

Query: 61 KKTSDCMAQCKCLSNGFCG 73
          +  SDC+  C C  NGFCG
Sbjct: 61 ELDSDCLPGCICRPNGFCG 79

BLAST of MS012402 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 5.3e-07
Identity = 35/79 (44.30%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 1  MGWKKFALVAIVGMLLMGASAQAGGAETVATEIQG-----RPRRMM--RGGICPRILMKC 60
          M WKK  ++A+VGML+M A  +   A   +  ++G      PR+MM     +CPRIL +C
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 KKTSDCMAQCKCLSNGFCG 73
              DCM  C CLSNGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

BLAST of MS012402 vs. ExPASy TrEMBL
Match: J3R9Z5 (Two inhibitor peptide topologies 3 OS=Momordica cochinchinensis OX=3674 GN=TIPTOP3 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.5e-06
Identity = 27/69 (39.13%), Postives = 40/69 (57.97%), Query Frame = 0

Query: 4  KKFALVAIVGMLLMGASAQAGGAETVATEIQGRPRRMMRGGICPRILMKCKKTSDCMAQC 63
          KK   V +V M+L+  S      +T+     GR +  + GG+CP+IL +C++ SDC   C
Sbjct: 4  KKILPVVLVAMMLVATSTGFNDGDTIDLISDGRAQIDINGGVCPKILQRCRRDSDCPGAC 63

Query: 64 KCLSNGFCG 73
           CL NG+CG
Sbjct: 64 ICLGNGYCG 72

BLAST of MS012402 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.8e-06
Identity = 33/75 (44.00%), Postives = 41/75 (54.67%), Query Frame = 0

Query: 1  MGWKKFALVAIVGMLLMGASAQA---GGAETVATEIQGRPRRMMRGGICPRILMKCKKTS 60
          M WKK ALVA++GMLLM    ++   G  E +   +            CPRILMKCK   
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEYSGHPGCPRILMKCKTDR 60

Query: 61 DCMAQCKCLSNGFCG 73
          DC+A C C  NG+CG
Sbjct: 61 DCLAGCTCKRNGYCG 75

BLAST of MS012402 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.3e-05
Identity = 37/92 (40.22%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 1  MGWKKFALVAIVGMLLMGASAQAGGAETV----ATEI--------QGRPRR-MMRGGI-- 60
          M  KK  +VAIVGM++M A+  +  +  V    +T +         G PR+ M++GG+  
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNNGCPRKIMIKGGVFK 60

Query: 61 -----CPRILMKCKKTSDCMAQCKCLSNGFCG 73
               CP+ILMKCK+ SDC+  C CL  GFCG
Sbjct: 61 EEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92