MS012401 (gene) Bitter gourd (TR) v1

Overview
NameMS012401
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionpre-mRNA-splicing factor CWC25-like isoform X2
Locationscaffold63: 419669 .. 421924 (+)
RNA-Seq ExpressionMS012401
SyntenyMS012401
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTTGTTATTAGTCCTAAGAGGTCTAGGAGGGACAGGAAACCATTAGAGGAGAAGTTATCCAGAAACTCTAGTTCTCACATTGATGATAATAAAGATCGGGATCAGAAGCATGAGCTTCGTCACAGAGGGCAAAATCTGATACCCCATGAGTCTTCATTAGCACTTGATTCTAAGCCAGAAGGTGGGGTTGTTAGCAAAGAGGCAAACAAGAACAGTGATGGGGGGAGGGATGGAGGGACTAAACATTCATTGAATCCCACTGAAGTATCTCGAGTGCGATCTTATTTTCAGGTTTGTGCTATATGTTAAAGCATTTTTTTCCCGATTAATTTTGATTTTTTTTTCTAATTTCAGGTTTTGAAAATTAACATGACTCTAGTTTGGTTTTGTCCTTGTCCACTATCTAACCAATTTTCATTATTTGATCTGAAGAATTATTGACAGCAACTTAAAGATCATTATTGGTATGCTTTTACCATTCTTTAATCTGTCATCCTAATCCCAAACCTTTTTGGTCTAGAATTAGATTGTTTGTTACTATGTATGAGGTTGGAAGCTTCAAAAGTTTTGTATTTTTATGTTTGAATTTAACTATAATTACTACTTATCCGTTGCTACTTTCTGTCGGTGTCGAAGTGGGACTGCTGCAAGATATGGGCATAACAATTATTTGACTTGATCTACATAGAGCTATTGCTGTACCTTCTCTGATATAAATAAATTCGTGTTGAGGTTTTCCTTTGAATTTCATGAAATTCATAACCTGCATCGTATTTCATTTGAATATGTACGATGCAAAATAGTTTAGCTCAGTAATAATTGTCTCTGCCCCTTCTCCTCCTGCCCTTTTCATTATTTGGTGCAATTTAGATTATGCAACCTTTTTGCTGGGCTGATGTAGTTGGAATCTTGTTCAGCCCTGTGCCTTCAAGTGCATCCGTGTATATAACTTCTTAGGCTTGCCTGTGCATGTCTTATAACAGCACGAGGAACGTGGTAATGCCGGGCAAGTTGGTCGAAGCTTTGGTCGGAGTGCAACTAGTGGTAGGTTTCCTTCCTTTTAAGCCCTGATTCCAACTTCTTTTTTTTGAGTTCTCTTCCTCATTTATTTCTTACTGTGCAACGTCATGAGTATATAGCAGTGCACAAAGACGACACTTTCTAGGTTATTCCTTATAACATTTTACTCTAAGCATGCCTATTAAGCATGTATTATTATTACTACTACTTTGATTCTCATGAAGACTACTCCAGATAATTGGAGCAGTTAACAAATACTGTTTTCCTCTTGTAGTAAATTCATGTACCCTTGTCGAAATGTTATGGAGACGGAGAAACTAGTGAAGTTTTGTTGTGGTGTAGTTCTGCCTTTCTCTTAAATGATCATATGGTCTTGGGAGGAAGTCATTCTACAAATAATCTCTCTGCCGGTGCCAAAAGGCAGAAATATAATTTTGTGGATAATGCACTTCTTATCTTTCTACTGTTCCGAAAATGTTACATATAAAATCCTTGTAGAGGTCAAATTTTTATCTAGACTTATATAGTGACAGTCTTGTACAGGAAAATTTAGAATCTCTCTTCAACCTTCTCGTTCCTGCTTCTCCACTTCTCTTGATGTAGTATTTTGTTTCTTTTTTTTTTTAATTTTTAGAGCGTGGTTGGTGGAAGGATTCGAAGGATGAGGATAACGATAGGGCACCTGGAAAGACAGCAACTTACAATTCACAGCAAAGAGATGAGATGCCACAAAGGGAGGATAATCACACGAGGCATCGTGATGGATCCTCGAAAGTGGAGGTTGATGCACCCACTCCTGCTCGGAAAAGGCCTGCATTTAGGGAAAAGAAGATTGCAGTAGACAGTGAAAATGTTGACAAAGCGGCTGTGGCATCAGAGACTAAGAAATCGAGTGATCCACACCATCCTGAGGACGGAAGGGAACGGAGAGAGGAAAGAGGCCGCGACACACGTTATTCTGAGAAACTTAATAAGCATGTCACTGGAGACATGGGTGCCAAAAGGGATGAGATAAAGAGAGGTGGCTACCCAGTGAGAGAAAGGTATGGGAATGGAGGTGGTGGGAACTACAGGGGAAGAGACAGATTCGGCGGGAGGCAAGGATATCGAGCGGGAGCGGGTGGTACTCGTGTAGAGAAATGGAAGCACGACCTGTTTCACGAGGCTAACCGGAGTCCAACTCCGAAGAATGAAGAGGACCAAATTGCCAAGGTTGAAGCTCTCCTCGCTTCT

mRNA sequence

CTTGTTGTTATTAGTCCTAAGAGGTCTAGGAGGGACAGGAAACCATTAGAGGAGAAGTTATCCAGAAACTCTAGTTCTCACATTGATGATAATAAAGATCGGGATCAGAAGCATGAGCTTCGTCACAGAGGGCAAAATCTGATACCCCATGAGTCTTCATTAGCACTTGATTCTAAGCCAGAAGGTGGGGTTGTTAGCAAAGAGGCAAACAAGAACAGTGATGGGGGGAGGGATGGAGGGACTAAACATTCATTGAATCCCACTGAAGTATCTCGAGTGCGATCTTATTTTCAGCACGAGGAACGTGGTAATGCCGGGCAAGTTGGTCGAAGCTTTGGTCGGAGTGCAACTAGTGAGCGTGGTTGGTGGAAGGATTCGAAGGATGAGGATAACGATAGGGCACCTGGAAAGACAGCAACTTACAATTCACAGCAAAGAGATGAGATGCCACAAAGGGAGGATAATCACACGAGGCATCGTGATGGATCCTCGAAAGTGGAGGTTGATGCACCCACTCCTGCTCGGAAAAGGCCTGCATTTAGGGAAAAGAAGATTGCAGTAGACAGTGAAAATGTTGACAAAGCGGCTGTGGCATCAGAGACTAAGAAATCGAGTGATCCACACCATCCTGAGGACGGAAGGGAACGGAGAGAGGAAAGAGGCCGCGACACACGTTATTCTGAGAAACTTAATAAGCATGTCACTGGAGACATGGGTGCCAAAAGGGATGAGATAAAGAGAGGTGGCTACCCAGTGAGAGAAAGGTATGGGAATGGAGGTGGTGGGAACTACAGGGGAAGAGACAGATTCGGCGGGAGGCAAGGATATCGAGCGGGAGCGGGTGGTACTCGTGTAGAGAAATGGAAGCACGACCTGTTTCACGAGGCTAACCGGAGTCCAACTCCGAAGAATGAAGAGGACCAAATTGCCAAGGTTGAAGCTCTCCTCGCTTCT

Coding sequence (CDS)

CTTGTTGTTATTAGTCCTAAGAGGTCTAGGAGGGACAGGAAACCATTAGAGGAGAAGTTATCCAGAAACTCTAGTTCTCACATTGATGATAATAAAGATCGGGATCAGAAGCATGAGCTTCGTCACAGAGGGCAAAATCTGATACCCCATGAGTCTTCATTAGCACTTGATTCTAAGCCAGAAGGTGGGGTTGTTAGCAAAGAGGCAAACAAGAACAGTGATGGGGGGAGGGATGGAGGGACTAAACATTCATTGAATCCCACTGAAGTATCTCGAGTGCGATCTTATTTTCAGCACGAGGAACGTGGTAATGCCGGGCAAGTTGGTCGAAGCTTTGGTCGGAGTGCAACTAGTGAGCGTGGTTGGTGGAAGGATTCGAAGGATGAGGATAACGATAGGGCACCTGGAAAGACAGCAACTTACAATTCACAGCAAAGAGATGAGATGCCACAAAGGGAGGATAATCACACGAGGCATCGTGATGGATCCTCGAAAGTGGAGGTTGATGCACCCACTCCTGCTCGGAAAAGGCCTGCATTTAGGGAAAAGAAGATTGCAGTAGACAGTGAAAATGTTGACAAAGCGGCTGTGGCATCAGAGACTAAGAAATCGAGTGATCCACACCATCCTGAGGACGGAAGGGAACGGAGAGAGGAAAGAGGCCGCGACACACGTTATTCTGAGAAACTTAATAAGCATGTCACTGGAGACATGGGTGCCAAAAGGGATGAGATAAAGAGAGGTGGCTACCCAGTGAGAGAAAGGTATGGGAATGGAGGTGGTGGGAACTACAGGGGAAGAGACAGATTCGGCGGGAGGCAAGGATATCGAGCGGGAGCGGGTGGTACTCGTGTAGAGAAATGGAAGCACGACCTGTTTCACGAGGCTAACCGGAGTCCAACTCCGAAGAATGAAGAGGACCAAATTGCCAAGGTTGAAGCTCTCCTCGCTTCT

Protein sequence

LVVISPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGVVSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWKDSKDEDNDRAPGKTATYNSQQRDEMPQREDNHTRHRDGSSKVEVDAPTPARKRPAFREKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGAKRDEIKRGGYPVRERYGNGGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRSPTPKNEEDQIAKVEALLAS
Homology
BLAST of MS012401 vs. NCBI nr
Match: XP_023548006.1 (pre-mRNA-splicing factor CWC25-like [Cucurbita pepo subsp. pepo] >XP_023548014.1 pre-mRNA-splicing factor CWC25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.1 bits (1237), Expect = 7.4e-132
Identity = 260/319 (81.50%), Postives = 289/319 (90.60%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ++ PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDVTPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK SLNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRSLNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKLSDPHQPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEEDQIAKVEALLAS
Sbjct: 323 PTPKNEEDQIAKVEALLAS 338

BLAST of MS012401 vs. NCBI nr
Match: XP_022942670.1 (pre-mRNA-splicing factor CWC25-like isoform X1 [Cucurbita moschata] >XP_022942671.1 pre-mRNA-splicing factor CWC25-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 480.7 bits (1236), Expect = 9.6e-132
Identity = 260/319 (81.50%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ+  PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDATPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK SLNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRSLNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKPSDPHQPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEEDQIAKVEALLAS
Sbjct: 323 PTPKNEEDQIAKVEALLAS 338

BLAST of MS012401 vs. NCBI nr
Match: KAG6600285.1 (hypothetical protein SDJN03_05518, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030943.1 hypothetical protein SDJN02_04980 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 479.2 bits (1232), Expect = 2.8e-131
Identity = 259/319 (81.19%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ+  PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDATPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK SLNPTE  R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRSLNPTEAPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKPSDPHQPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEEDQIAKVEALLAS
Sbjct: 323 PTPKNEEDQIAKVEALLAS 338

BLAST of MS012401 vs. NCBI nr
Match: XP_023521659.1 (pre-mRNA-splicing factor CWC25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.0 bits (1229), Expect = 6.3e-131
Identity = 259/319 (81.19%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRK LEEKLSRNSSSH++DNKDRDQKHELRH GQ++ PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKTLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDVTPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK SLNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRSLNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKLSDPHQPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEEDQIAKVEALLAS
Sbjct: 323 PTPKNEEDQIAKVEALLAS 338

BLAST of MS012401 vs. NCBI nr
Match: XP_022984546.1 (pre-mRNA-splicing factor CWC25 isoform X1 [Cucurbita maxima])

HSP 1 Score: 476.1 bits (1224), Expect = 2.4e-130
Identity = 258/319 (80.88%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ++ PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDVTPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK +LNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRALNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKLSDPHPPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEE QIAKVEALLAS
Sbjct: 323 PTPKNEEAQIAKVEALLAS 338

BLAST of MS012401 vs. ExPASy TrEMBL
Match: A0A6J1FPJ0 (pre-mRNA-splicing factor CWC25-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447638 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.7e-132
Identity = 260/319 (81.50%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ+  PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDATPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK SLNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRSLNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKPSDPHQPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEEDQIAKVEALLAS
Sbjct: 323 PTPKNEEDQIAKVEALLAS 338

BLAST of MS012401 vs. ExPASy TrEMBL
Match: A0A6J1J5K1 (pre-mRNA-splicing factor CWC25 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482807 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.2e-130
Identity = 258/319 (80.88%), Postives = 288/319 (90.28%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ++ PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDVTPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK +LNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRALNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKLSDPHPPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGN-GGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRS 304
           KRDE+KRGGY VRERYGN GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRS
Sbjct: 263 KRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRS 322

Query: 305 PTPKNEEDQIAKVEALLAS 319
           PTPKNEE QIAKVEALLAS
Sbjct: 323 PTPKNEEAQIAKVEALLAS 338

BLAST of MS012401 vs. ExPASy TrEMBL
Match: A0A6J1FQX4 (pre-mRNA-splicing factor CWC25-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447638 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.2e-125
Identity = 250/318 (78.62%), Postives = 278/318 (87.42%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ+  PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDATPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK SLNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRSLNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKPSDPHQPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGNGGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRSP 304
           KRDE+KR           GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRSP
Sbjct: 263 KRDEMKR-----------GGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRSP 322

Query: 305 TPKNEEDQIAKVEALLAS 319
           TPKNEEDQIAKVEALLAS
Sbjct: 323 TPKNEEDQIAKVEALLAS 326

BLAST of MS012401 vs. ExPASy TrEMBL
Match: A0A6J1JAV5 (pre-mRNA-splicing factor CWC25 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482807 PE=4 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.8e-123
Identity = 248/318 (77.99%), Postives = 278/318 (87.42%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRDRKPLEEKLSRNSSSH++DNKDRDQKHELRH GQ++ PHESSLALDS PEGG 
Sbjct: 23  SPKRSRRDRKPLEEKLSRNSSSHVEDNKDRDQKHELRHEGQDVTPHESSLALDSHPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
           VSK AN+NSD  R+GGTK +LNPTEV R RSYFQH++RG+AGQVGRSFGRS++S RGWWK
Sbjct: 83  VSKGANRNSD-EREGGTKRALNPTEVPRSRSYFQHDKRGSAGQVGRSFGRSSSSGRGWWK 142

Query: 125 DSKDED--NDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK++D  +DRA GKTATYNSQ+RDE+PQ  +EDNHTR RDGSSK+EVD+  PARKRPAF
Sbjct: 143 DSKEQDKNSDRASGKTATYNSQRRDEIPQTVKEDNHTRTRDGSSKLEVDSSAPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKLNKHVTGDMGA 244
           REKKI  D+EN +KA +ASE+KK SDPH P+DGRERREERGRDTRYS+KL+KHVTGDM +
Sbjct: 203 REKKITPDNENFEKAGMASESKKLSDPHPPQDGRERREERGRDTRYSDKLSKHVTGDMAS 262

Query: 245 KRDEIKRGGYPVRERYGNGGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANRSP 304
           KRDE+KR           GGGGNYRGRDRFGGRQGY A +GGTRVEKWKHDLFHEANRSP
Sbjct: 263 KRDEMKR-----------GGGGNYRGRDRFGGRQGYPA-SGGTRVEKWKHDLFHEANRSP 322

Query: 305 TPKNEEDQIAKVEALLAS 319
           TPKNEE QIAKVEALLAS
Sbjct: 323 TPKNEEAQIAKVEALLAS 326

BLAST of MS012401 vs. ExPASy TrEMBL
Match: A0A0A0KV12 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000750 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.7e-121
Identity = 251/320 (78.44%), Postives = 276/320 (86.25%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRS+RDRK LEEKLSRNSSSH++DNK+RDQKHELR  GQ++IPHESSLAL+SKPEGG 
Sbjct: 23  SPKRSKRDRKTLEEKLSRNSSSHVEDNKERDQKHELRLVGQDMIPHESSLALESKPEGG- 82

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
               AN+NSD GRDGGTKHS+NPTEV R RSYFQH+ER NAGQVGRSFGRSATSERGWWK
Sbjct: 83  AGIGANRNSD-GRDGGTKHSVNPTEVPRSRSYFQHDERSNAGQVGRSFGRSATSERGWWK 142

Query: 125 DSK--DEDNDRAPGKTATYNSQQRDEMPQ--REDNHTRHRDGSSKVEVDAPTPARKRPAF 184
           DSK  D+D+DRA GK+ TY SQQRDE PQ  RED   RH DGSSK+EVDA TPARKRPAF
Sbjct: 143 DSKDPDKDSDRASGKSLTYKSQQRDEKPQAARED---RHHDGSSKLEVDALTPARKRPAF 202

Query: 185 REKKIAVDSENVDKAAVASETKKSSDPHHPEDGRERREERGRDTRYSEKL-NKHVTGDMG 244
           REKKI VD+E+ +K A+  E+KKS+D H  ++GRERREERGRD RYSEKL NKH  GDM 
Sbjct: 203 REKKITVDNEDGEKVAMVPESKKSNDLHQIQEGRERREERGRDPRYSEKLNNKHSNGDMA 262

Query: 245 AKRDEIKRGGYPVRERYGNG-GGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEANR 304
           +KRDE+KR GYPVRERYGNG GGGNYRGRDRFGGRQGYR    GTRVEKWKHDLFHEANR
Sbjct: 263 SKRDEMKRVGYPVRERYGNGSGGGNYRGRDRFGGRQGYR--TSGTRVEKWKHDLFHEANR 322

Query: 305 SPTPKNEEDQIAKVEALLAS 319
           SPTPKNEEDQIAKVEALL+S
Sbjct: 323 SPTPKNEEDQIAKVEALLSS 335

BLAST of MS012401 vs. TAIR 10
Match: AT3G19650.1 (cyclin-related )

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-20
Identity = 107/321 (33.33%), Postives = 157/321 (48.91%), Query Frame = 0

Query: 5   SPKRSRRDRKPLEEKLSRNSSSHIDDNKDRDQKHELRHRGQNLIPHESSLALDSKPEGGV 64
           SPKRSRRD KP  E++       + D  D +     ++  Q+L   ++ L LD+      
Sbjct: 24  SPKRSRRDGKPEAERVLSKKDLDVRDGTDTE-----KNSRQSL--RDAPLELDAHG---- 83

Query: 65  VSKEANKNSDGGRDGGTKHSLNPTEVSRVRSYFQHEERGNAGQVGRSFGRSATSERGWWK 124
            S++ ++    G    TK + + ++V R R Y QH++R + G+V     R  TS RG W+
Sbjct: 84  -SRKDSEKKHSGHHETTKAAPHLSQVPRSRPYSQHDDRRSDGKV----DRRPTSVRGSWR 143

Query: 125 DSKDEDNDRAPGKTATYNSQQRDEMPQREDNHTRHRDGSSKVEVDAPTPARKRPAFREKK 184
            S+D+ N RA     + + +  D+   R D      D    +       +RKRPAFREKK
Sbjct: 144 SSRDQSNRRAGDDEKSQHRKDEDKSSWRHDRFRESDDTQGAL-------SRKRPAFREKK 203

Query: 185 IAVDSENVDKAAVASETKKSSDPHHPEDGRE----RREERGRDTRYSEKLNKHVTGDMGA 244
           I            A ET  ++D    EDG++     R +  R+ R +    +H    MG 
Sbjct: 204 I------------AEETGNNTDRTRTEDGKDTNLNNRRQNERNWRSNTHSERHERPAMG- 263

Query: 245 KRDEIKRGGYPVRERYGNGGGGNYRG-RDRF--GGRQGYRAGAGGTRVEKWKHDLFHEAN 304
            RD +    +   +  G G   +Y+  RDRF   GR G+ +G+     +KW HDLF EAN
Sbjct: 264 -RDRV----WNRDDERGAGSRQSYQADRDRFNGNGRSGF-SGSWTRNEKKWDHDLFDEAN 302

Query: 305 RSPTPKNEEDQIAKVEALLAS 319
           +SP    EE+QIAKVEALLAS
Sbjct: 324 KSPAKATEEEQIAKVEALLAS 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023548006.17.4e-13281.50pre-mRNA-splicing factor CWC25-like [Cucurbita pepo subsp. pepo] >XP_023548014.1... [more]
XP_022942670.19.6e-13281.50pre-mRNA-splicing factor CWC25-like isoform X1 [Cucurbita moschata] >XP_02294267... [more]
KAG6600285.12.8e-13181.19hypothetical protein SDJN03_05518, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023521659.16.3e-13181.19pre-mRNA-splicing factor CWC25-like [Cucurbita pepo subsp. pepo][more]
XP_022984546.12.4e-13080.88pre-mRNA-splicing factor CWC25 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FPJ04.7e-13281.50pre-mRNA-splicing factor CWC25-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1J5K11.2e-13080.88pre-mRNA-splicing factor CWC25 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FQX47.2e-12578.62pre-mRNA-splicing factor CWC25-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JAV51.8e-12377.99pre-mRNA-splicing factor CWC25 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0KV121.7e-12178.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000750 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G19650.11.3e-2033.33cyclin-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 147..251
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 120..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..282
NoneNo IPR availablePANTHERPTHR36364OS03G0203000 PROTEINcoord: 5..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012401.1MS012401.1mRNA