MS012399 (gene) Bitter gourd (TR) v1

Overview


Name	MS012399
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Adenylyl-sulfate kinase
Location	scaffold63: 407611 .. 409722 (-)
RNA-Seq Expression	MS012399
Synteny	MS012399

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCGATTGGGGGAAATTCGTTGCCGGGATTTGTGCGGCCGTCGCTCCACCAGCATTCGTTGTCGGCGCCGGCGTTTGGATCCGCGAGGTTTCCGGCTGTGATTAGCTTCGGGTCGAGGGTGATTTTGCGGGCTGAGGTGGAGAATAAGGATTCGAGGGCGGTGATTGTGAATGGTTTGGGCAAGCGTGAGTGTGATTGTGAGACTGAATTCGATGCGACCTTGGGAAATGGACATGCAGTGAACTCTGGTATTGCATCGATTCTGATGTCTATTTTAATCTGTTCTTGATTCATGGACCTGTGCTTTGGTTCTTAAGATTCTTAGTATATTATAAGGTATGGAATTGGATGTGATCTTCTCTGTCAATCATATCCTTGAAATTGTAGGTGTTCTGTCTGTTTTGATTGGTTTTATAAGACTGTGTAAATAGGTTTGATATGTTTCTTTTTAGTGTTGATGTATATGATTTGGTTGCAGAAGATTCAAATAATTTTCATGTTGAAATTGGCTTATAATGTGTGTTCTTCATGGTATCAGTTTCTGTTCTTTAGATGAAGGTTTCCAGGTTACTGATATGCCTTTTTTCAATCAGGGCCAAATCTTGGGGTGTTGTCCACAGTAGGTAATTCTACAAATATCAAGTGGCATGACTGTTCAGTTGGAAAGCTTGAAAGACAAAGCCTTCTCAAACAGAAAGGATGTGTTATATGGATTACAGGTCTTAGTGGCTCAGGTTTGTCATTATAGCTTGATCAAACGAAATGGTTATCAAACGGCACCTTAATAATCAAGTTACTATATATATATGAATATATGATAATCGTAACTGAAAGAACCTAGAGTTTTGATTGTATCCGGACGAAATTAGTTTTGGAGTAAGAGGTGTTCACGGGTTGGGTTGGGGATCTTTTGGACCCAATCTAATGTTAAGTTGGAGATTTGCTCAACCCAAAAATCTCCACCCAAACAACCCTTGTTTGATGATAGACCCAAGAAAAGGTGAAATTGTTAGAATAAAATTAGTGAGTTGGGTTAGTTCGGGTACTTAGGTCATTTGTTCAGAATTTTGTGAACAAAATAGCGAAATTGCTAGCCTAGAGTTGGGTCAGTGACTTTTTGGATCCTACCCAATTTTCCTAAGTTTGCTTAGCTAACTTGCAGGGAAGAGCTCTGTGGCCTGTGCCTTGAGTCAGAGCTTACACAGAATGGGAAAGCTGGCTTATATTCTTGATGGGGACAATGTCAGGCATGGCTTGAACCGCGACCTCGGTTTTAAAGCAGAAGATCGTGCTGAGAACATAAGGAGGATTGGTAAGTCTCGCTTTGGCAGTTCAGTTCAGATACAACACTTTGAATAGGGCTGTTCATGTTCATGATTTCTAACTATACCTTTCCTGTAAATTAGGCGAGGTTGCAAAACTGTTCGCAGATGCTGGAGTTATCTGCATTGCCAGTCTGATATCACCATATCGAAGGGATCGAGATGCCTGCCGTGCCATTTTGCCTGATGGATATTTTATTGAGGTTGTTCTTCGTTTATGGATGCCATAGAATTTCAGCAGCCTTCTATCTCCATACCTGACAAAAAGTGCTGAAAATATGTAGGTGTTCATGGATATTTCTCTTGAAGTTTGTGAAGCAAGAGATTCGAAAGGACTGTATAAGCTTGCACGGGCTGGGAAGATCAAAGGTTATTGTAACTCGTCAATTCTCAAAATTCAAATACTGATAAGATATAATCTTGTTTTGGTCTCTAAACTTTTGGCTCGAAGTTGTTATCGAATGCGCTTTAACTATGCAAAATCTTGCTGTTTCTGCAGGCTTTACCGGCATTGATGATCCATACGAACTACCATTGAATTGTGAGGTAATTCCAGTTTCTGGAGGAAAATGGAACACCTTTTTCAAACTAAGTTGCAAAAGATCAATGCTTCTAATGTTTTGCAACATATGCTGAGGTTTAGTTAGAGAATGAAGATTAAGAATAAATAATGATATATTTTTGTTGAACTGTTTGGGTTTCAGATAGTACTGAATGGAGGTTCCCCATGTGAAATGGCAGAGAAAGTAATGAGTTACCTTGAGGAGAATGGCTTTCTGCAGGCT

mRNA sequence

ATGGTTGCGATTGGGGGAAATTCGTTGCCGGGATTTGTGCGGCCGTCGCTCCACCAGCATTCGTTGTCGGCGCCGGCGTTTGGATCCGCGAGGTTTCCGGCTGTGATTAGCTTCGGGTCGAGGGTGATTTTGCGGGCTGAGGTGGAGAATAAGGATTCGAGGGCGGTGATTGTGAATGGTTTGGGCAAGCGTGAGTGTGATTGTGAGACTGAATTCGATGCGACCTTGGGAAATGGACATGCAGTGAACTCTGGGCCAAATCTTGGGGTGTTGTCCACAGTAGGTAATTCTACAAATATCAAGTGGCATGACTGTTCAGTTGGAAAGCTTGAAAGACAAAGCCTTCTCAAACAGAAAGGATGTGTTATATGGATTACAGGTCTTAGTGGCTCAGGGAAGAGCTCTGTGGCCTGTGCCTTGAGTCAGAGCTTACACAGAATGGGAAAGCTGGCTTATATTCTTGATGGGGACAATGTCAGGCATGGCTTGAACCGCGACCTCGGTTTTAAAGCAGAAGATCGTGCTGAGAACATAAGGAGGATTGGCGAGGTTGCAAAACTGTTCGCAGATGCTGGAGTTATCTGCATTGCCAGTCTGATATCACCATATCGAAGGGATCGAGATGCCTGCCGTGCCATTTTGCCTGATGGATATTTTATTGAGGTTGTGTTCATGGATATTTCTCTTGAAGTTTGTGAAGCAAGAGATTCGAAAGGACTGTATAAGCTTGCACGGGCTGGGAAGATCAAAGGCTTTACCGGCATTGATGATCCATACGAACTACCATTGAATTGTGAGATAGTACTGAATGGAGGTTCCCCATGTGAAATGGCAGAGAAAGTAATGAGTTACCTTGAGGAGAATGGCTTTCTGCAGGCT

Coding sequence (CDS)

ATGGTTGCGATTGGGGGAAATTCGTTGCCGGGATTTGTGCGGCCGTCGCTCCACCAGCATTCGTTGTCGGCGCCGGCGTTTGGATCCGCGAGGTTTCCGGCTGTGATTAGCTTCGGGTCGAGGGTGATTTTGCGGGCTGAGGTGGAGAATAAGGATTCGAGGGCGGTGATTGTGAATGGTTTGGGCAAGCGTGAGTGTGATTGTGAGACTGAATTCGATGCGACCTTGGGAAATGGACATGCAGTGAACTCTGGGCCAAATCTTGGGGTGTTGTCCACAGTAGGTAATTCTACAAATATCAAGTGGCATGACTGTTCAGTTGGAAAGCTTGAAAGACAAAGCCTTCTCAAACAGAAAGGATGTGTTATATGGATTACAGGTCTTAGTGGCTCAGGGAAGAGCTCTGTGGCCTGTGCCTTGAGTCAGAGCTTACACAGAATGGGAAAGCTGGCTTATATTCTTGATGGGGACAATGTCAGGCATGGCTTGAACCGCGACCTCGGTTTTAAAGCAGAAGATCGTGCTGAGAACATAAGGAGGATTGGCGAGGTTGCAAAACTGTTCGCAGATGCTGGAGTTATCTGCATTGCCAGTCTGATATCACCATATCGAAGGGATCGAGATGCCTGCCGTGCCATTTTGCCTGATGGATATTTTATTGAGGTTGTGTTCATGGATATTTCTCTTGAAGTTTGTGAAGCAAGAGATTCGAAAGGACTGTATAAGCTTGCACGGGCTGGGAAGATCAAAGGCTTTACCGGCATTGATGATCCATACGAACTACCATTGAATTGTGAGATAGTACTGAATGGAGGTTCCCCATGTGAAATGGCAGAGAAAGTAATGAGTTACCTTGAGGAGAATGGCTTTCTGCAGGCT

Protein sequence

MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNGLGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA

Homology

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BLAST of MS012399 vs. NCBI nr
Match: XP_022149302.1 (adenylyl-sulfate kinase 3-like [Momordica charantia])

HSP 1 Score: 589.3 bits (1518), Expect = 1.8e-164
Identity = 291/293 (99.32%), Postives = 292/293 (99.66%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG
Sbjct: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60

Query: 61  LGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKG 120
           LGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKG
Sbjct: 61  LGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKG 120

Query: 121 CVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRR 180
           CV+WITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRR
Sbjct: 121 CVVWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRR 180

Query: 181 IGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSKGL 240
           IGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMDISLEVCEARDSKGL
Sbjct: 181 IGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDISLEVCEARDSKGL 240

Query: 241 YKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA 294
           YKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA
Sbjct: 241 YKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA 292

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BLAST of MS012399 vs. NCBI nr
Match: XP_038903366.1 (adenylyl-sulfate kinase 3-like [Benincasa hispida])

HSP 1 Score: 483.0 bits (1242), Expect = 1.8e-132
Identity = 248/299 (82.94%), Postives = 262/299 (87.63%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVN- 60
           MVAIGG SLPGF+ PSLH  S S  A G ARFP  ISF S V+ R  VE KDSR VIVN 
Sbjct: 1   MVAIGGISLPGFLPPSLHHQSFSPSAIGFARFPDRISFRSSVVWRDGVEKKDSRVVIVNG 60

Query: 61  ---GLGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLL 120
              GLGK E  CETEFD TLGNGHA NSG N GVLSTVGNSTNIKWH+CSVGK E+QSLL
Sbjct: 61  KVDGLGKSE--CETEFDTTLGNGHAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLL 120

Query: 121 KQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAE 180
           KQKGCVIWITGLSGSGKS+VACALSQSL++MGKLAYILDGDNVRHGLNRDLGFKAEDRAE
Sbjct: 121 KQKGCVIWITGLSGSGKSTVACALSQSLYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAE 180

Query: 181 NIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARD 240
           NIRR+GEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+ LEVCEARD
Sbjct: 181 NIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVPLEVCEARD 240

Query: 241 SKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           +KGLYKLARAGKIKGFTGIDDPYE+PLNCEI+L  NGGSP EMA KV+SYLE+ GFLQA
Sbjct: 241 AKGLYKLARAGKIKGFTGIDDPYEVPLNCEILLKHNGGSPSEMAGKVLSYLEQKGFLQA 296

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BLAST of MS012399 vs. NCBI nr
Match: XP_023551902.1 (adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 1.8e-132
Identity = 246/297 (82.83%), Postives = 261/297 (87.88%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGG SLP F+ PSLH+ S S PA G ARFP  I   S V L  EVE   SRA IVN 
Sbjct: 1   MVAIGGISLPRFIAPSLHRQSFSPPAIGFARFPNRIRLRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  L--GKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQ 120
              G  + DCET+FDATLGNGHA NSG NLGVLSTVGNSTNIKWH+CSVGK+ERQSLL+Q
Sbjct: 61  KVDGLVKSDCETDFDATLGNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKS VACAL+Q L++MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSK 240
           RRIGEVAKLFA+AGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+SLEVCEARD+K
Sbjct: 181 RRIGEVAKLFANAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVSLEVCEARDAK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           GLYKLARAGKIKGFTGIDDPYE+PLNCEIVL  NGGSPCEMAEKVMSYLE+ GFLQA
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. NCBI nr
Match: XP_022922441.1 (adenylyl-sulfate kinase 3-like [Cucurbita moschata] >XP_022922442.1 adenylyl-sulfate kinase 3-like [Cucurbita moschata])

HSP 1 Score: 481.9 bits (1239), Expect = 4.0e-132
Identity = 247/297 (83.16%), Postives = 260/297 (87.54%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGG SLP FV PSLH+ S S PA G ARF   ISF S V L  EVE   SRA IVN 
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFQNRISFRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  L--GKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQ 120
              G  + DCET+FDATL NGHA NSG NLGVLSTVGNSTNIKWH+CSVGK+ERQSLL+Q
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKS VACAL+Q L++MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSK 240
           RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+SLEVCEARD+K
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVSLEVCEARDAK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           GLYKLARAGKIKGFTGIDDPYE+PLNCEIVL  NGGSPCEMAEKV SYLE+ GFLQA
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVTSYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. NCBI nr
Match: KAG6576911.1 (Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014937.1 Adenylyl-sulfate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.9 bits (1239), Expect = 4.0e-132
Identity = 246/297 (82.83%), Postives = 260/297 (87.54%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGG SLP FV PSLH+ S S PA G ARFP  I   S V L  EVE   SRA IVN 
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPDRIRLRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  L--GKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQ 120
              G  + DCET+FDATL NGHA NSG NLGVLSTVGNSTNIKWH+CSVGK+ERQSLL+Q
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKS VACAL+Q L++MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSK 240
           RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+SLEVCEARD+K
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVSLEVCEARDAK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           GLYKLARAGKIKGFTGIDDPYE+PLNCEIVL  NGGSPCEMAEKV+SYLE+ GFLQA
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVLSYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. ExPASy Swiss-Prot
Match: Q43295 (Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1 PE=1 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 5.0e-93
Identity = 168/219 (76.71%), Postives = 188/219 (85.84%), Query Frame = 0


Query: 78  NGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVA 137
           NGH   +G   G LSTVGNSTNIKWH+CSV K++RQ LL QKGCVIW+TGLSGSGKS++A
Sbjct: 62  NGH---TGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLA 121

Query: 138 CALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIA 197
           CAL+Q L++ GKL YILDGDNVRHGLNRDL FKAEDRAENIRR+GEVAKLFADAG+ICIA
Sbjct: 122 CALNQMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIA 181

Query: 198 SLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDD 257
           SLISPYR DRDACR++LP+G F+E VFMD+ L VCEARD KGLYKLARAGKIKGFTGIDD
Sbjct: 182 SLISPYRTDRDACRSLLPEGDFVE-VFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDD 241

Query: 258 PYELPLNCEIVL---NGGSPCEMAEKVMSYLEENGFLQA 294
           PYE PLNCEI L    G SP EMAEKV+ YL+  G+LQA
Sbjct: 242 PYEPPLNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

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BLAST of MS012399 vs. ExPASy Swiss-Prot
Match: O49204 (Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 3.7e-88
Identity = 166/234 (70.94%), Postives = 190/234 (81.20%), Query Frame = 0


Query: 67  DCETEFDATLGNGHAVNSGPNLGVL--STVGNSTNIKWHDCSVGKLERQSLLKQKGCVIW 126
           DC +  D++L N    N  P   +L  +TVGNSTNI WH C+V K ERQ  L+Q+GCVIW
Sbjct: 83  DCNSNSDSSLNN---CNGFPGKKILQTTTVGNSTNILWHKCAVEKSERQEPLQQRGCVIW 142

Query: 127 ITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEV 186
           ITGLSGSGKS++ACALS+ LH  GKL YILDGDNVRHGLN DL FKAEDRAENIRRIGEV
Sbjct: 143 ITGLSGSGKSTLACALSRGLHAKGKLTYILDGDNVRHGLNSDLSFKAEDRAENIRRIGEV 202

Query: 187 AKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSKGLYKLA 246
           AKLFADAGVICIASLISPYR+  DACR++LP+G FIE VFMD+ L+VCEARD KGLYKLA
Sbjct: 203 AKLFADAGVICIASLISPYRKPPDACRSLLPEGDFIE-VFMDVPLKVCEARDPKGLYKLA 262

Query: 247 RAGKIKGFTGIDDPYELPLNCEIVLNG-----GSPCEMAEKVMSYLEENGFLQA 294
           RAGKIKGFTGIDDPYE PL  EIVL+       SPC++A+ V+SYLEENG+L+A
Sbjct: 263 RAGKIKGFTGIDDPYEPPLKSEIVLHQKLGMCDSPCDLADIVISYLEENGYLKA 312

[Close]

BLAST of MS012399 vs. ExPASy Swiss-Prot
Match: Q9SRW7 (Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.5e-84
Identity = 152/207 (73.43%), Postives = 177/207 (85.51%), Query Frame = 0


Query: 91  LSTVGNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKL 150
           +STVGNSTNI W +  +GK ERQ LL QKGCV+WITGLSGSGKS++AC+LS+ L+  GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 151 AYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDAC 210
           +YILDGDN+RHGLN+DLGFKAEDR ENIRR+GEVAKLFADAG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 211 RAILPDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVLN 270
           R ++ +  FIE VFM++SL++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI L 
Sbjct: 121 REMIQNSSFIE-VFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELK 180

Query: 271 G-----GSPCEMAEKVMSYLEENGFLQ 293
                  SP  MAE+V+SYLE+ GFLQ
Sbjct: 181 EKEGECPSPVAMAEEVISYLEDKGFLQ 206

[Close]

BLAST of MS012399 vs. ExPASy Swiss-Prot
Match: Q84JF0 (Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.3e-81
Identity = 148/218 (67.89%), Postives = 172/218 (78.90%), Query Frame = 0


Query: 95  GNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYIL 154
           G   NI WHDC V K +RQ L+KQKGCVIWITGLSGSGKSS+ACALS++LH  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 155 DGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAIL 214
           DGDNVRHGLN DL F+A+DRAENIRR+GEVAKLFAD+G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 215 PDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL----- 274
           P G FIE VFMD+ L VCEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV+     
Sbjct: 204 PQGDFIE-VFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRD 263

Query: 275 ---------------NGGSPCEMAEKVMSYLEENGFLQ 293
                          +  S CEMA+ V+SYL++NG+L+
Sbjct: 264 KGLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

[Close]

BLAST of MS012399 vs. ExPASy Swiss-Prot
Match: O49196 (Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2 PE=1 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 5.7e-81
Identity = 145/200 (72.50%), Postives = 167/200 (83.50%), Query Frame = 0


Query: 99  NIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDN 158
           NI WH+ S+ + +RQ LL+QKGCV+WITGLSGSGKS+VACALS++L   GKL Y LDGDN
Sbjct: 94  NIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLDGDN 153

Query: 159 VRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGY 218
           VRHGLNRDL FKAE R ENIRRIGEVAKLFAD GVICIASLISPYRRDRDACR++LPDG 
Sbjct: 154 VRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYRRDRDACRSLLPDGD 213

Query: 219 FIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL------NGG 278
           F+E VFMD+ L VCE+RD KGLYKLARAGKIKGFTGIDDPYE P+NCE+VL         
Sbjct: 214 FVE-VFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNCEVVLKHTGDDESC 273

Query: 279 SPCEMAEKVMSYLEENGFLQ 293
           SP +MAE ++SYL+  G+L+
Sbjct: 274 SPRQMAENIISYLQNKGYLE 292

[Close]

BLAST of MS012399 vs. ExPASy TrEMBL
Match: A0A6J1D7K1 (Adenylyl-sulfate kinase OS=Momordica charantia OX=3673 GN=LOC111017750 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 8.6e-165
Identity = 291/293 (99.32%), Postives = 292/293 (99.66%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG
Sbjct: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60

Query: 61  LGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKG 120
           LGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKG
Sbjct: 61  LGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKG 120

Query: 121 CVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRR 180
           CV+WITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRR
Sbjct: 121 CVVWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRR 180

Query: 181 IGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSKGL 240
           IGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMDISLEVCEARDSKGL
Sbjct: 181 IGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDISLEVCEARDSKGL 240

Query: 241 YKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA 294
           YKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA
Sbjct: 241 YKLARAGKIKGFTGIDDPYELPLNCEIVLNGGSPCEMAEKVMSYLEENGFLQA 292

[Close]

BLAST of MS012399 vs. ExPASy TrEMBL
Match: A0A6J1E450 (Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430457 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.9e-132
Identity = 247/297 (83.16%), Postives = 260/297 (87.54%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGG SLP FV PSLH+ S S PA G ARF   ISF S V L  EVE   SRA IVN 
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFQNRISFRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  L--GKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQ 120
              G  + DCET+FDATL NGHA NSG NLGVLSTVGNSTNIKWH+CSVGK+ERQSLL+Q
Sbjct: 61  KVDGLVKSDCETDFDATLSNGHARNSGKNLGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKS VACAL+Q L++MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSK 240
           RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+SLEVCEARD+K
Sbjct: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVSLEVCEARDAK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           GLYKLARAGKIKGFTGIDDPYE+PLNCEIVL  NGGSPCEMAEKV SYLE+ GFLQA
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVTSYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. ExPASy TrEMBL
Match: A0A6J1J3W8 (Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483165 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.3e-131
Identity = 245/297 (82.49%), Postives = 260/297 (87.54%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVNG 60
           MVAIGG SLP FV PSLH+ S S PA G ARFP  IS+ S V L  EVE   SRA IVN 
Sbjct: 1   MVAIGGISLPRFVAPSLHRQSFSPPAIGFARFPNRISYRSTVALLDEVEKTGSRAAIVNR 60

Query: 61  L--GKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQ 120
              G  + DCET+FDATLGNGHA NSG  LGVLSTVGNSTNIKWH+CSVGK+ERQSLL+Q
Sbjct: 61  KVDGLVKSDCETDFDATLGNGHARNSGKILGVLSTVGNSTNIKWHECSVGKIERQSLLEQ 120

Query: 121 KGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180
           KGCVIWITGLSGSGKS VACAL+Q L++MGKLAYILDGDNVRHGLNRDLGFKAEDRAENI
Sbjct: 121 KGCVIWITGLSGSGKSCVACALNQILYKMGKLAYILDGDNVRHGLNRDLGFKAEDRAENI 180

Query: 181 RRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSK 240
           +RIGEVAKLFA AGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+ LEVCEARD+K
Sbjct: 181 KRIGEVAKLFAHAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVPLEVCEARDAK 240

Query: 241 GLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           GLYKLARAGKIKGFTGIDDPYE+PLNCEIVL  NGGSPCEMAEKVMSYLE+ GFLQA
Sbjct: 241 GLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKNNGGSPCEMAEKVMSYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. ExPASy TrEMBL
Match: A0A1S3BYH9 (Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 8.4e-128
Identity = 242/299 (80.94%), Postives = 255/299 (85.28%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVN- 60
           MV+IG  SLPG V PSLH       A G ARFP  ISF S V+LR EVE K SR VIVN 
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  ---GLGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLL 120
              GLGK E  CETEFD  LGNG A NSG N GVLSTVGNSTNIKWH+CSVGK E+QSLL
Sbjct: 61  KVDGLGKSE--CETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLL 120

Query: 121 KQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAE 180
           KQKGCVIW TGLSGSGKS+VACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAE
Sbjct: 121 KQKGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAE 180

Query: 181 NIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARD 240
           NIRR+GEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+ LEVCEARD
Sbjct: 181 NIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVPLEVCEARD 240

Query: 241 SKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           +KGLYKLARAGKIKGFTGIDDPYE+PLNCEIVL   GGSP EMAEKV++YLE+ GFLQA
Sbjct: 241 AKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. ExPASy TrEMBL
Match: A0A5A7TN80 (Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00500 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 8.4e-128
Identity = 242/299 (80.94%), Postives = 255/299 (85.28%), Query Frame = 0


Query: 1   MVAIGGNSLPGFVRPSLHQHSLSAPAFGSARFPAVISFGSRVILRAEVENKDSRAVIVN- 60
           MV+IG  SLPG V PSLH       A G ARFP  ISF S V+LR EVE K SR VIVN 
Sbjct: 1   MVSIGRISLPGSVPPSLHHQCSPPSAIGFARFPDRISFRSSVVLRDEVEKKGSRVVIVNG 60

Query: 61  ---GLGKRECDCETEFDATLGNGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLL 120
              GLGK E  CETEFD  LGNG A NSG N GVLSTVGNSTNIKWH+CSVGK E+QSLL
Sbjct: 61  KVDGLGKSE--CETEFDTALGNGRAGNSGKNHGVLSTVGNSTNIKWHECSVGKNEKQSLL 120

Query: 121 KQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAE 180
           KQKGCVIW TGLSGSGKS+VACALSQSL +MGKLAYILDGDNVRHGLNRDLGFKAEDRAE
Sbjct: 121 KQKGCVIWFTGLSGSGKSTVACALSQSLCKMGKLAYILDGDNVRHGLNRDLGFKAEDRAE 180

Query: 181 NIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARD 240
           NIRR+GEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE VFMD+ LEVCEARD
Sbjct: 181 NIRRVGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGYFIE-VFMDVPLEVCEARD 240

Query: 241 SKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL--NGGSPCEMAEKVMSYLEENGFLQA 294
           +KGLYKLARAGKIKGFTGIDDPYE+PLNCEIVL   GGSP EMAEKV++YLE+ GFLQA
Sbjct: 241 AKGLYKLARAGKIKGFTGIDDPYEVPLNCEIVLKHTGGSPSEMAEKVLNYLEQKGFLQA 296

[Close]

BLAST of MS012399 vs. TAIR 10
Match: AT2G14750.1 (APS kinase )

HSP 1 Score: 342.4 bits (877), Expect = 3.5e-94
Identity = 168/219 (76.71%), Postives = 188/219 (85.84%), Query Frame = 0


Query: 78  NGHAVNSGPNLGVLSTVGNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVA 137
           NGH   +G   G LSTVGNSTNIKWH+CSV K++RQ LL QKGCVIW+TGLSGSGKS++A
Sbjct: 62  NGH---TGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLA 121

Query: 138 CALSQSLHRMGKLAYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIA 197
           CAL+Q L++ GKL YILDGDNVRHGLNRDL FKAEDRAENIRR+GEVAKLFADAG+ICIA
Sbjct: 122 CALNQMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIA 181

Query: 198 SLISPYRRDRDACRAILPDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDD 257
           SLISPYR DRDACR++LP+G F+E VFMD+ L VCEARD KGLYKLARAGKIKGFTGIDD
Sbjct: 182 SLISPYRTDRDACRSLLPEGDFVE-VFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDD 241

Query: 258 PYELPLNCEIVL---NGGSPCEMAEKVMSYLEENGFLQA 294
           PYE PLNCEI L    G SP EMAEKV+ YL+  G+LQA
Sbjct: 242 PYEPPLNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA 276

[Close]

BLAST of MS012399 vs. TAIR 10
Match: AT3G03900.1 (adenosine-5'-phosphosulfate (APS) kinase 3 )

HSP 1 Score: 313.5 bits (802), Expect = 1.8e-85
Identity = 152/207 (73.43%), Postives = 177/207 (85.51%), Query Frame = 0


Query: 91  LSTVGNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKL 150
           +STVGNSTNI W +  +GK ERQ LL QKGCV+WITGLSGSGKS++AC+LS+ L+  GKL
Sbjct: 1   MSTVGNSTNIFWQESPIGKTERQKLLNQKGCVVWITGLSGSGKSTLACSLSRELNNRGKL 60

Query: 151 AYILDGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDAC 210
           +YILDGDN+RHGLN+DLGFKAEDR ENIRR+GEVAKLFADAG+ICIASLISPYR+DRDAC
Sbjct: 61  SYILDGDNLRHGLNKDLGFKAEDRVENIRRVGEVAKLFADAGLICIASLISPYRKDRDAC 120

Query: 211 RAILPDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVLN 270
           R ++ +  FIE VFM++SL++CEARD KGLYKLARAGKIKGFTGIDDPYE PLNCEI L 
Sbjct: 121 REMIQNSSFIE-VFMNMSLQLCEARDPKGLYKLARAGKIKGFTGIDDPYESPLNCEIELK 180

Query: 271 G-----GSPCEMAEKVMSYLEENGFLQ 293
                  SP  MAE+V+SYLE+ GFLQ
Sbjct: 181 EKEGECPSPVAMAEEVISYLEDKGFLQ 206

[Close]

BLAST of MS012399 vs. TAIR 10
Match: AT5G67520.1 (adenosine-5'-phosphosulfate (APS) kinase 4 )

HSP 1 Score: 303.1 bits (775), Expect = 2.4e-82
Identity = 148/218 (67.89%), Postives = 172/218 (78.90%), Query Frame = 0


Query: 95  GNSTNIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYIL 154
           G   NI WHDC V K +RQ L+KQKGCVIWITGLSGSGKSS+ACALS++LH  GKL+YIL
Sbjct: 84  GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 143

Query: 155 DGDNVRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAIL 214
           DGDNVRHGLN DL F+A+DRAENIRR+GEVAKLFAD+G+ICIASLISPYR +R ACRA+L
Sbjct: 144 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 203

Query: 215 PDGYFIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL----- 274
           P G FIE VFMD+ L VCEARD KGLYK ARAGKIKGFTG+DDPYE PL+CEIV+     
Sbjct: 204 PQGDFIE-VFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVIQNSRD 263

Query: 275 ---------------NGGSPCEMAEKVMSYLEENGFLQ 293
                          +  S CEMA+ V+SYL++NG+L+
Sbjct: 264 KGLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLK 300

[Close]

BLAST of MS012399 vs. TAIR 10
Match: AT4G39940.1 (APS-kinase 2 )

HSP 1 Score: 302.4 bits (773), Expect = 4.0e-82
Identity = 145/200 (72.50%), Postives = 167/200 (83.50%), Query Frame = 0


Query: 99  NIKWHDCSVGKLERQSLLKQKGCVIWITGLSGSGKSSVACALSQSLHRMGKLAYILDGDN 158
           NI WH+ S+ + +RQ LL+QKGCV+WITGLSGSGKS+VACALS++L   GKL Y LDGDN
Sbjct: 94  NIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKSTVACALSKALFERGKLTYTLDGDN 153

Query: 159 VRHGLNRDLGFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRRDRDACRAILPDGY 218
           VRHGLNRDL FKAE R ENIRRIGEVAKLFAD GVICIASLISPYRRDRDACR++LPDG 
Sbjct: 154 VRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVICIASLISPYRRDRDACRSLLPDGD 213

Query: 219 FIEVVFMDISLEVCEARDSKGLYKLARAGKIKGFTGIDDPYELPLNCEIVL------NGG 278
           F+E VFMD+ L VCE+RD KGLYKLARAGKIKGFTGIDDPYE P+NCE+VL         
Sbjct: 214 FVE-VFMDVPLHVCESRDPKGLYKLARAGKIKGFTGIDDPYEAPVNCEVVLKHTGDDESC 273

Query: 279 SPCEMAEKVMSYLEENGFLQ 293
           SP +MAE ++SYL+  G+L+
Sbjct: 274 SPRQMAENIISYLQNKGYLE 292

The following BLAST results are available for this feature:

BLAST of MS012399 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149302.1	1.8e-164	99.32	adenylyl-sulfate kinase 3-like [Momordica charantia]	[more]
XP_038903366.1	1.8e-132	82.94	adenylyl-sulfate kinase 3-like [Benincasa hispida]	[more]
XP_023551902.1	1.8e-132	82.83	adenylyl-sulfate kinase 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022922441.1	4.0e-132	83.16	adenylyl-sulfate kinase 3-like [Cucurbita moschata] >XP_022922442.1 adenylyl-sul...	[more]
KAG6576911.1	4.0e-132	82.83	Adenylyl-sulfate kinase 1, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of MS012399 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43295	5.0e-93	76.71	Adenylyl-sulfate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK1...	[more]
O49204	3.7e-88	70.94	Adenylyl-sulfate kinase, chloroplastic OS=Catharanthus roseus OX=4058 GN=AKN PE=...	[more]
Q9SRW7	2.5e-84	73.43	Adenylyl-sulfate kinase 3 OS=Arabidopsis thaliana OX=3702 GN=APK3 PE=1 SV=1	[more]
Q84JF0	3.3e-81	67.89	Adenylyl-sulfate kinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK4...	[more]
O49196	5.7e-81	72.50	Adenylyl-sulfate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APK2...	[more]

BLAST of MS012399 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D7K1	8.6e-165	99.32	Adenylyl-sulfate kinase OS=Momordica charantia OX=3673 GN=LOC111017750 PE=3 SV=1	[more]
A0A6J1E450	1.9e-132	83.16	Adenylyl-sulfate kinase OS=Cucurbita moschata OX=3662 GN=LOC111430457 PE=3 SV=1	[more]
A0A6J1J3W8	1.3e-131	82.49	Adenylyl-sulfate kinase OS=Cucurbita maxima OX=3661 GN=LOC111483165 PE=3 SV=1	[more]
A0A1S3BYH9	8.4e-128	80.94	Adenylyl-sulfate kinase OS=Cucumis melo OX=3656 GN=LOC103494812 PE=3 SV=1	[more]
A0A5A7TN80	8.4e-128	80.94	Adenylyl-sulfate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]

BLAST of MS012399 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G14750.1	3.5e-94	76.71	APS kinase	[more]
AT3G03900.1	1.8e-85	73.43	adenosine-5'-phosphosulfate (APS) kinase 3	[more]
AT5G67520.1	2.4e-82	67.89	adenosine-5'-phosphosulfate (APS) kinase 4	[more]
AT4G39940.1	4.0e-82	72.50	APS-kinase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002891	Adenylyl-sulfate kinase	TIGRFAM	TIGR00455	TIGR00455	coord: 102..273 e-value: 2.9E-72 score: 240.2
IPR002891	Adenylyl-sulfate kinase	HAMAP	MF_00065	Adenylyl_sulf_kinase	coord: 118..289 score: 33.489201
IPR002891	Adenylyl-sulfate kinase	CDD	cd02027	APSK	coord: 122..270 e-value: 2.2712E-96 score: 278.592
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 96..293 e-value: 7.6E-89 score: 298.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 118..287
None	No IPR available	PFAM	PF01583	APS_kinase	coord: 119..270 e-value: 8.3E-69 score: 230.5
None	No IPR available	PANTHER	PTHR11055	BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE	coord: 58..293
None	No IPR available	PANTHER	PTHR11055:SF55	ADENYLYL-SULFATE KINASE 1, CHLOROPLASTIC	coord: 58..293

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012399.1	MS012399.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000103	sulfate assimilation
molecular_function	GO:0004020	adenylylsulfate kinase activity
molecular_function	GO:0005524	ATP binding