MS012327 (gene) Bitter gourd (TR) v1

Overview
NameMS012327
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription factor bHLH143-like
Locationscaffold63: 24128 .. 25177 (-)
RNA-Seq ExpressionMS012327
SyntenyMS012327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGGGGCAGATAACTCTCAGCTTGATTTTGAGCATTTTGCTTGGCAACTTCATAATCATAATTCCATGAATACATCTATAGAGATCAAGCAACAGGAAAGCTTGCCTCCGTATATCAACCCTGGAAATTGCTTGTTTTCCACATGTATAGGAATGCAAGGGTTTGCAATTCCACCATTACCCAGTTTTAAAGTGGAACAGACAAATGTTGTTCATGGGTCGCGTCAATGCTTATCCCCTCATTTCCAGAACTCACTTGGTACTCCAAGTCCTTGTCAGAAAGAAAAGGAATCTCTGCATTATGCTGGACCTAGTGGGATGCCTGTTTCAAAATCAACTAATGGGTCGTATTCGAAAGGTTTTCTTATCTTTGATCAATCTGGGAATCAGAAGAGATTGATGTATGCTCCTATGTGTCCTCCTCTCTATTTTCCCTCAACAGTTGCTGAAAACCATGGTCCTGGTTTGCTAGAAGAATGGATAGCTGGAGGCATTGATTCTGTGAAGTATTTTCCAAATACGCTGTCCAATGAGAATTACATAGCTGGTGAAGAGAGTGAAATGCATGAAAACACAGAAGAAATAGATGCCTTGCTTTACTCAGACGATGATGGTACTGGTTATAGTAGTGATGATGAAGTGACTAGCACAGGACATTCTCCAGAGATGATCAATGAGCATTATGAGAAGGAAGAACAATGTCAAGAAACTACTACAGAAGTTGCTAGTTCTGATGGGCCAAGAAAAAGGCAGAGAGTGCATGATGGAGGGTCCATAAAATCACTGCCAATTGCTACTGTTGCTGGTGAAAGAGTTGAACTACAGAAGTTTGCCAGTGATGCAGAATCAAGCTGTGGCATGGTCCATGAGGAAGAAGCTGGAACAGATGTTGATTTCTGCACTTCTTCTTGCAAAAAAGACAGGATCAAGGAGACGTTGAGAGTGCTTGAGAGATTAGTTCCTGGTGCAAAGGGCAAGGACCCATTGTTGGTCATCGATGAAGCTATAGAATACTTGAAGTCCTTAAAACACGAAGCTAGCACGCTA

mRNA sequence

ATGGTTGGGGCAGATAACTCTCAGCTTGATTTTGAGCATTTTGCTTGGCAACTTCATAATCATAATTCCATGAATACATCTATAGAGATCAAGCAACAGGAAAGCTTGCCTCCGTATATCAACCCTGGAAATTGCTTGTTTTCCACATGTATAGGAATGCAAGGGTTTGCAATTCCACCATTACCCAGTTTTAAAGTGGAACAGACAAATGTTGTTCATGGGTCGCGTCAATGCTTATCCCCTCATTTCCAGAACTCACTTGGTACTCCAAGTCCTTGTCAGAAAGAAAAGGAATCTCTGCATTATGCTGGACCTAGTGGGATGCCTGTTTCAAAATCAACTAATGGGTCGTATTCGAAAGGTTTTCTTATCTTTGATCAATCTGGGAATCAGAAGAGATTGATGTATGCTCCTATGTGTCCTCCTCTCTATTTTCCCTCAACAGTTGCTGAAAACCATGGTCCTGGTTTGCTAGAAGAATGGATAGCTGGAGGCATTGATTCTGTGAAGTATTTTCCAAATACGCTGTCCAATGAGAATTACATAGCTGGTGAAGAGAGTGAAATGCATGAAAACACAGAAGAAATAGATGCCTTGCTTTACTCAGACGATGATGGTACTGGTTATAGTAGTGATGATGAAGTGACTAGCACAGGACATTCTCCAGAGATGATCAATGAGCATTATGAGAAGGAAGAACAATGTCAAGAAACTACTACAGAAGTTGCTAGTTCTGATGGGCCAAGAAAAAGGCAGAGAGTGCATGATGGAGGGTCCATAAAATCACTGCCAATTGCTACTGTTGCTGGTGAAAGAGTTGAACTACAGAAGTTTGCCAGTGATGCAGAATCAAGCTGTGGCATGGTCCATGAGGAAGAAGCTGGAACAGATGTTGATTTCTGCACTTCTTCTTGCAAAAAAGACAGGATCAAGGAGACGTTGAGAGTGCTTGAGAGATTAGTTCCTGGTGCAAAGGGCAAGGACCCATTGTTGGTCATCGATGAAGCTATAGAATACTTGAAGTCCTTAAAACACGAAGCTAGCACGCTA

Coding sequence (CDS)

ATGGTTGGGGCAGATAACTCTCAGCTTGATTTTGAGCATTTTGCTTGGCAACTTCATAATCATAATTCCATGAATACATCTATAGAGATCAAGCAACAGGAAAGCTTGCCTCCGTATATCAACCCTGGAAATTGCTTGTTTTCCACATGTATAGGAATGCAAGGGTTTGCAATTCCACCATTACCCAGTTTTAAAGTGGAACAGACAAATGTTGTTCATGGGTCGCGTCAATGCTTATCCCCTCATTTCCAGAACTCACTTGGTACTCCAAGTCCTTGTCAGAAAGAAAAGGAATCTCTGCATTATGCTGGACCTAGTGGGATGCCTGTTTCAAAATCAACTAATGGGTCGTATTCGAAAGGTTTTCTTATCTTTGATCAATCTGGGAATCAGAAGAGATTGATGTATGCTCCTATGTGTCCTCCTCTCTATTTTCCCTCAACAGTTGCTGAAAACCATGGTCCTGGTTTGCTAGAAGAATGGATAGCTGGAGGCATTGATTCTGTGAAGTATTTTCCAAATACGCTGTCCAATGAGAATTACATAGCTGGTGAAGAGAGTGAAATGCATGAAAACACAGAAGAAATAGATGCCTTGCTTTACTCAGACGATGATGGTACTGGTTATAGTAGTGATGATGAAGTGACTAGCACAGGACATTCTCCAGAGATGATCAATGAGCATTATGAGAAGGAAGAACAATGTCAAGAAACTACTACAGAAGTTGCTAGTTCTGATGGGCCAAGAAAAAGGCAGAGAGTGCATGATGGAGGGTCCATAAAATCACTGCCAATTGCTACTGTTGCTGGTGAAAGAGTTGAACTACAGAAGTTTGCCAGTGATGCAGAATCAAGCTGTGGCATGGTCCATGAGGAAGAAGCTGGAACAGATGTTGATTTCTGCACTTCTTCTTGCAAAAAAGACAGGATCAAGGAGACGTTGAGAGTGCTTGAGAGATTAGTTCCTGGTGCAAAGGGCAAGGACCCATTGTTGGTCATCGATGAAGCTATAGAATACTTGAAGTCCTTAAAACACGAAGCTAGCACGCTA

Protein sequence

MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCIGMQGFAIPPLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHYAGPSGMPVSKSTNGSYSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL
Homology
BLAST of MS012327 vs. NCBI nr
Match: XP_022984572.1 (transcription factor bHLH143-like [Cucurbita maxima])

HSP 1 Score: 560.5 bits (1443), Expect = 1.1e-155
Identity = 287/354 (81.07%), Postives = 303/354 (85.59%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHN +SMN  +EIKQQESL   IN GNC+  TC+ GMQGFAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNRDSMNAPVEIKQQESLQGNINCGNCILPTCVGGMQGFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSFKVEQ NVV  SRQCL PHFQNSLGTP P QK KES+HY  AGPSGMPVSKS NGS
Sbjct: 61  PLPSFKVEQLNVVQESRQCLPPHFQNSLGTPMPWQKGKESMHYAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPM PP+Y PST AE    G L EE  AG I+SVKYFPNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMYPPVYIPSTFAETKRCGWLEEEGAAGEINSVKYFPNTL 180

Query: 181 SNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQ 240
           SNENY+   ESEMHENTEEIDALLYSD DGTG SSDDEVTSTGHSPEMI++H EKEEQCQ
Sbjct: 181 SNENYVGDGESEMHENTEEIDALLYSDYDGTGCSSDDEVTSTGHSPEMIHDHCEKEEQCQ 240

Query: 241 ETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGT 300
           ETTTE ASS+GPRKRQRVHDGG +KSLPIAT +  RVELQKF +DAESSCGMVHEEE GT
Sbjct: 241 ETTTEAASSNGPRKRQRVHDGGYVKSLPIATASSARVELQKFVNDAESSCGMVHEEEVGT 300

Query: 301 DVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DVDFC SSCKKDRIKETLRVLE LVP AKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 DVDFCRSSCKKDRIKETLRVLESLVPDAKGKDPLLVIDEAIDYLKSLKHEASAL 354

BLAST of MS012327 vs. NCBI nr
Match: XP_022922510.1 (transcription factor bHLH143-like [Cucurbita moschata])

HSP 1 Score: 558.9 bits (1439), Expect = 3.1e-155
Identity = 287/354 (81.07%), Postives = 301/354 (85.03%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHN +SMN  +EIKQQESL   IN GNC+  TC+ GMQGFAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNRDSMNAPVEIKQQESLQRNINCGNCILPTCVGGMQGFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSFKVEQ NVV  SRQCL PHFQNSLGTP P QK KES+HY  AGPSGMPVSKS NGS
Sbjct: 61  PLPSFKVEQLNVVQESRQCLPPHFQNSLGTPMPWQKGKESMHYAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPMCPP+Y PST AE    G L EE  AG IDSVKYFPNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMCPPVYIPSTFAETKRCGWLEEEGAAGDIDSVKYFPNTL 180

Query: 181 SNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQ 240
           SNENY+A  ESEMHENTEEIDALLYSD D T  SSDDEVTSTGHSPEMI+EH EKEEQCQ
Sbjct: 181 SNENYVADGESEMHENTEEIDALLYSDYDCTSCSSDDEVTSTGHSPEMIHEHCEKEEQCQ 240

Query: 241 ETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGT 300
           ETTTE ASS+GPRKRQRVHDGG +KSLP  T +  RVELQK  +DAESSCGMVHEEEAGT
Sbjct: 241 ETTTEAASSNGPRKRQRVHDGGYVKSLPSTTASSARVELQKIVNDAESSCGMVHEEEAGT 300

Query: 301 DVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DVDFC SSCKKDRIKETLRVLE LVP AKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 DVDFCHSSCKKDRIKETLRVLESLVPDAKGKDPLLVIDEAIDYLKSLKHEASAL 354

BLAST of MS012327 vs. NCBI nr
Match: KAG6576874.1 (Transcription factor basic helix-loop-helix 143, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557.0 bits (1434), Expect = 1.2e-154
Identity = 286/354 (80.79%), Postives = 301/354 (85.03%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHN +SMN  +EIKQQESL   IN GNC+  TC+ GMQGFAIP
Sbjct: 73  MVGTDNSRLDFEHFAWQLHNRDSMNAPVEIKQQESLQRNINCGNCILPTCVGGMQGFAIP 132

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSFKVEQ NVV  SRQCL PHFQNSLGTP P QK KES+HY  AGPSGMPVSKS NGS
Sbjct: 133 PLPSFKVEQLNVVQESRQCLPPHFQNSLGTPMPWQKGKESMHYAHAGPSGMPVSKSNNGS 192

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPMCPP+Y PST AE    G L EE  AG I+SVKYFPNTL
Sbjct: 193 YPKGFLIFDQSGNQKRLMYAPMCPPVYIPSTFAETKRCGWLEEEGAAGDINSVKYFPNTL 252

Query: 181 SNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQ 240
           SNENY+A  ESEMHENTEEIDALLYSD D T  SSDDEVTSTGHSPEMI+EH EKEEQCQ
Sbjct: 253 SNENYVADGESEMHENTEEIDALLYSDYDCTSCSSDDEVTSTGHSPEMIHEHCEKEEQCQ 312

Query: 241 ETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGT 300
           ETTTE ASS+GPRKRQRVHDGG +KSLP  T +  RVELQK  +DAESSCGMVHEEEAGT
Sbjct: 313 ETTTEAASSNGPRKRQRVHDGGYVKSLPSTTASSARVELQKIVNDAESSCGMVHEEEAGT 372

Query: 301 DVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DVDFC SSCKKDRIKETLRVLE LVP AKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 373 DVDFCHSSCKKDRIKETLRVLESLVPDAKGKDPLLVIDEAIDYLKSLKHEASAL 426

BLAST of MS012327 vs. NCBI nr
Match: XP_023552238.1 (transcription factor bHLH143-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551.2 bits (1419), Expect = 6.4e-153
Identity = 284/354 (80.23%), Postives = 300/354 (84.75%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHN +SMN  +EIKQQESL   IN GNC+  T + GMQGFAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNRDSMNAPVEIKQQESLQRNINCGNCILPTRVGGMQGFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSFKVEQ NVV  SRQCL PHFQNSLGTP P QK KES+HY  AGPSGMPVSKS NGS
Sbjct: 61  PLPSFKVEQLNVVQESRQCLPPHFQNSLGTPMPWQKGKESMHYAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPMCPP+Y PST AE    G L EE  AG I+SVKYFPNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMCPPVYIPSTFAETKRCGWLEEEGAAGDINSVKYFPNTL 180

Query: 181 SNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQ 240
           SNENY+A  ESEMHENTEEIDALLYSD D T  SSDDEVTSTGHSPEMI+EH EK+EQCQ
Sbjct: 181 SNENYVADGESEMHENTEEIDALLYSDYDCTSCSSDDEVTSTGHSPEMIHEHCEKDEQCQ 240

Query: 241 ETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGT 300
           ETTTE ASS+GPRKRQRVHDGG +KSLP  T +  RVELQK  +DAESSCGMVHEEEAGT
Sbjct: 241 ETTTEAASSNGPRKRQRVHDGGYVKSLPSTTASSARVELQKIVNDAESSCGMVHEEEAGT 300

Query: 301 DVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DVDFC SSCKKDRIKETLRVLE LVP AKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 DVDFCHSSCKKDRIKETLRVLESLVPDAKGKDPLLVIDEAIDYLKSLKHEASAL 354

BLAST of MS012327 vs. NCBI nr
Match: XP_038903965.1 (transcription factor bHLH143-like [Benincasa hispida])

HSP 1 Score: 550.8 bits (1418), Expect = 8.4e-153
Identity = 284/355 (80.00%), Postives = 305/355 (85.92%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHNHNSMN S+EIKQQE+    IN GNC+FSTC+ GMQGFAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNHNSMNASVEIKQQENFQTNINHGNCIFSTCVGGMQGFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPS +VEQ NVV GS QCLSP F NSLGT  PCQK+KES+H+  AGPSGMPVSKS NGS
Sbjct: 61  PLPSLEVEQLNVVQGSHQCLSPDFLNSLGTFMPCQKKKESVHFAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEE-WIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPMCPP+Y PSTVAE    G LEE   AG I S+KYFPNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMCPPVYIPSTVAETKRCGWLEEKEAAGDIHSIKYFPNTL 180

Query: 181 SNENYIA-GEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQC 240
           ++ENY+A GE SEMHENTEEIDALLYSD DGTG SSDDEVTSTGHSPEMI+EH EKEEQC
Sbjct: 181 ASENYVADGESSEMHENTEEIDALLYSDYDGTGCSSDDEVTSTGHSPEMIDEHCEKEEQC 240

Query: 241 QETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAG 300
           QETTTEVASSDGPRKRQRV DGG IKSLP+A V+  RVE Q FA DAESSC MV +EE+G
Sbjct: 241 QETTTEVASSDGPRKRQRVLDGGYIKSLPVAMVSCARVESQNFADDAESSCAMVLKEESG 300

Query: 301 TDVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           T VDFC SSCKKDRIKETLRVLE LVPGAKGKDPLLVIDEAI+YLKSLKHEA+ L
Sbjct: 301 TYVDFCNSSCKKDRIKETLRVLENLVPGAKGKDPLLVIDEAIDYLKSLKHEANAL 355

BLAST of MS012327 vs. ExPASy Swiss-Prot
Match: Q9FY69 (Transcription factor bHLH143 OS=Arabidopsis thaliana OX=3702 GN=BHLH143 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.9e-31
Identity = 116/345 (33.62%), Postives = 170/345 (49.28%), Query Frame = 0

Query: 28  IEIKQQESLPPYINPGNCLFSTCIGMQGFAIPPLPSFKVEQTNVVHGSRQCLSPHFQNSL 87
           ++ KQQ+ LP  +NP  C+         +  P +P  ++ +        + L P FQ  L
Sbjct: 3   LDTKQQKWLPLGLNPQACVQDKA---TEYFRPGIPFPELGKVYAAEHQFRYLQPPFQALL 62

Query: 88  G--TPSPCQKEKESLHYAGPSGMPVSKSTNGSYSKGFLIFDQSGNQKRLMYAPMCPPLYF 147
                  C K+   L+    +G     +   S  K F++FDQSG Q RL+      PL F
Sbjct: 63  SRYDQQSCGKQVSCLNGRSSNGAAPEGALKSS-RKRFIVFDQSGEQTRLLQCGF--PLRF 122

Query: 148 PSTVAENHGPGLLEEWIAGGIDSVKYF-------PNTLSNENYIAGEE-SEMHENTEEID 207
           PS++    G       I G +   K F          L +E++  GEE SEMHE+TEEI+
Sbjct: 123 PSSMDAERGN------ILGALHPEKGFSKDHAIQEKILQHEDHENGEEDSEMHEDTEEIN 182

Query: 208 ALLYS-DDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETTTEV----ASSDGP-RKR 267
           ALLYS DDD   + SDDEV STGHSP  +     +++ C  TT E+    ++ DGP  KR
Sbjct: 183 ALLYSDDDDNDDWESDDEVMSTGHSPFTV-----EQQACNITTEELDETESTVDGPLLKR 242

Query: 268 QRVHDGGSIKSLP-------IATVAGERVELQKFASDAESSCGMVHEEEAGTDVDFCTSS 327
           Q++ D     S P       +  ++ E +     +S  E+  G+  E+            
Sbjct: 243 QKLLDHSYRDSSPSLVGTTKVKGLSDENLPESNISSKQETGSGLSDEQS----------- 302

Query: 328 CKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEAST 350
            +KD+I   LR+LE +VPGAKGK+ LL++DEAI+YLK LK   ++
Sbjct: 303 -RKDKIHTALRILESVVPGAKGKEALLLLDEAIDYLKLLKQSLNS 318

BLAST of MS012327 vs. ExPASy Swiss-Prot
Match: Q9FMF4 (Transcription factor SAC51 OS=Arabidopsis thaliana OX=3702 GN=SAC51 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.2e-29
Identity = 118/333 (35.44%), Postives = 159/333 (47.75%), Query Frame = 0

Query: 31  KQQESLPPYINPGNCLFSTCIGMQGFAIPPLPSFKVEQTNVVHGSRQCLS-PHFQNSLGT 90
           K+Q  LP  ++P  C F   +G     +P +P  ++ +        +CL  P FQ+ L  
Sbjct: 5   KRQRDLPLGLSPQAC-FKDIVGRS--VLPRIPLPELGKLYAAKLQARCLQPPPFQSLL-- 64

Query: 91  PSPCQKEKESL-----------HYAGPSGMPVSKSTNGSYSKGFLIFDQSGNQKRLMYAP 150
              C  +KES              A  +          S  K  LIFDQSG+Q RL+  P
Sbjct: 65  ---CSHDKESYGKRFSRSDMRSWCAAATTTTTPLGALESSQKRLLIFDQSGDQTRLLQCP 124

Query: 151 MCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSNENYIAGEESEMHENTEEIDA 210
              PL FPS  A    P  L E    GI+  K F       +   G ESEMHE+TEEI+A
Sbjct: 125 F--PLRFPSHAAAE--PVKLSE--LQGIE--KAFKEDGEEFHKSDGTESEMHEDTEEINA 184

Query: 211 LLYSDDD-GTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETTTEVASSDGPRKRQ----R 270
           LLYSDDD      SDDEV STGHSP      Y  E  C +   E+   DGP KRQ    +
Sbjct: 185 LLYSDDDYDDDCESDDEVMSTGHSP------YPNEGVCNK--RELEEIDGPCKRQKLLDK 244

Query: 271 VHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDVDFCTSSCKKDRIKET 330
           V++   + SL + T +  ++    F  D +         +  T         KKD+I+  
Sbjct: 245 VNNISDLSSL-VGTESSTQLNGSSFLKDKKLPESKTISTKEDTGSGLSNEQSKKDKIRTA 304

Query: 331 LRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHE 347
           L++LE +VPGAKG + LL++DEAI+YLK LK +
Sbjct: 305 LKILESVVPGAKGNEALLLLDEAIDYLKLLKRD 312

BLAST of MS012327 vs. ExPASy Swiss-Prot
Match: Q9FGB0 (Transcription factor bHLH145 OS=Arabidopsis thaliana OX=3702 GN=BHLH145 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.8e-15
Identity = 77/240 (32.08%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 110 VSKSTNGSYSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSV 169
           +SK+      K FL+FDQSG+Q  L+ A      +   T+ ++  P + EE      D  
Sbjct: 97  ISKAEEQCSQKRFLVFDQSGDQTTLLLASDIRKSF--ETLKQHACPDMKEELQRSNKD-- 156

Query: 170 KYFPNTLSNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHY 229
                           E ++ E++EE++ALLYS+D+    S +DEVTS  HSP ++    
Sbjct: 157 -----LFVCHGMQGNSEPDLKEDSEELNALLYSEDESGYCSEEDEVTSADHSPSIVVSGR 216

Query: 230 EKEEQCQETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMV 289
           E ++      T + S   P          + K   I   + E +       DAESSCG  
Sbjct: 217 EDQK------TFLGSYGQPL---------NAKKRKILETSNESMR------DAESSCGSC 276

Query: 290 HEEEAGTDVDFCTSSCK--KDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEA 348
                        SS K  +++I ET+ +L  +VPG +  DP+LVID AI+YLKSLK EA
Sbjct: 277 DNTRISFLKRSKLSSNKIGEEKIFETVSLLRSVVPGEELVDPILVIDRAIDYLKSLKMEA 306

BLAST of MS012327 vs. ExPASy Swiss-Prot
Match: Q9ASX9 (Transcription factor bHLH144 OS=Arabidopsis thaliana OX=3702 GN=BHLH144 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.2e-05
Identity = 58/231 (25.11%), Postives = 91/231 (39.39%), Query Frame = 0

Query: 120 KGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSNE 179
           K F+IFDQ+ ++ ++MY P        +    N       E++ G   +           
Sbjct: 53  KNFVIFDQTYDRSQVMYHPELTHKLMNTPSLNNLASTFQNEYVGGSYGNY---------G 112

Query: 180 NYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETT 239
           NY     S   E+  EIDALL +D+D      ++     G S E+        +    T 
Sbjct: 113 NYEQEVSSSYQEDPNEIDALLSADEDYEENDDNEGEEDGGDSEEVSTARTSSRDYGNTTA 172

Query: 240 TEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDVD 299
               SS G       ++  S K              Q  +  A SS    + +  G    
Sbjct: 173 ESCCSSYGYNNN---NNNNSRK--------------QSLSGSASSS----NNDGKG---- 232

Query: 300 FCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
                  + ++K+ + VL R+VPG +  +   V+DEA++YLKSLK EA  L
Sbjct: 233 -------RKKMKKMMGVLRRIVPGGEQMNTACVLDEAVQYLKSLKIEAQKL 242

BLAST of MS012327 vs. ExPASy TrEMBL
Match: A0A6J1J2I7 (transcription factor bHLH143-like OS=Cucurbita maxima OX=3661 GN=LOC111482828 PE=4 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 5.1e-156
Identity = 287/354 (81.07%), Postives = 303/354 (85.59%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHN +SMN  +EIKQQESL   IN GNC+  TC+ GMQGFAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNRDSMNAPVEIKQQESLQGNINCGNCILPTCVGGMQGFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSFKVEQ NVV  SRQCL PHFQNSLGTP P QK KES+HY  AGPSGMPVSKS NGS
Sbjct: 61  PLPSFKVEQLNVVQESRQCLPPHFQNSLGTPMPWQKGKESMHYAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPM PP+Y PST AE    G L EE  AG I+SVKYFPNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMYPPVYIPSTFAETKRCGWLEEEGAAGEINSVKYFPNTL 180

Query: 181 SNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQ 240
           SNENY+   ESEMHENTEEIDALLYSD DGTG SSDDEVTSTGHSPEMI++H EKEEQCQ
Sbjct: 181 SNENYVGDGESEMHENTEEIDALLYSDYDGTGCSSDDEVTSTGHSPEMIHDHCEKEEQCQ 240

Query: 241 ETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGT 300
           ETTTE ASS+GPRKRQRVHDGG +KSLPIAT +  RVELQKF +DAESSCGMVHEEE GT
Sbjct: 241 ETTTEAASSNGPRKRQRVHDGGYVKSLPIATASSARVELQKFVNDAESSCGMVHEEEVGT 300

Query: 301 DVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DVDFC SSCKKDRIKETLRVLE LVP AKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 DVDFCRSSCKKDRIKETLRVLESLVPDAKGKDPLLVIDEAIDYLKSLKHEASAL 354

BLAST of MS012327 vs. ExPASy TrEMBL
Match: A0A6J1E8Z4 (transcription factor bHLH143-like OS=Cucurbita moschata OX=3662 GN=LOC111430490 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-155
Identity = 287/354 (81.07%), Postives = 301/354 (85.03%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCI-GMQGFAIP 60
           MVG DNS+LDFEHFAWQLHN +SMN  +EIKQQESL   IN GNC+  TC+ GMQGFAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNRDSMNAPVEIKQQESLQRNINCGNCILPTCVGGMQGFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSFKVEQ NVV  SRQCL PHFQNSLGTP P QK KES+HY  AGPSGMPVSKS NGS
Sbjct: 61  PLPSFKVEQLNVVQESRQCLPPHFQNSLGTPMPWQKGKESMHYAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPMCPP+Y PST AE    G L EE  AG IDSVKYFPNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMCPPVYIPSTFAETKRCGWLEEEGAAGDIDSVKYFPNTL 180

Query: 181 SNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQ 240
           SNENY+A  ESEMHENTEEIDALLYSD D T  SSDDEVTSTGHSPEMI+EH EKEEQCQ
Sbjct: 181 SNENYVADGESEMHENTEEIDALLYSDYDCTSCSSDDEVTSTGHSPEMIHEHCEKEEQCQ 240

Query: 241 ETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGT 300
           ETTTE ASS+GPRKRQRVHDGG +KSLP  T +  RVELQK  +DAESSCGMVHEEEAGT
Sbjct: 241 ETTTEAASSNGPRKRQRVHDGGYVKSLPSTTASSARVELQKIVNDAESSCGMVHEEEAGT 300

Query: 301 DVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DVDFC SSCKKDRIKETLRVLE LVP AKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 DVDFCHSSCKKDRIKETLRVLESLVPDAKGKDPLLVIDEAIDYLKSLKHEASAL 354

BLAST of MS012327 vs. ExPASy TrEMBL
Match: A0A6J1KRG3 (transcription factor bHLH143-like OS=Cucurbita maxima OX=3661 GN=LOC111497979 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.5e-152
Identity = 281/353 (79.60%), Postives = 303/353 (85.84%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCIGMQGFAI-P 60
           MVG DNSQL F HFAWQL NH+SMN S+EIKQQESL  +INPGNC+FSTC+GMQG AI P
Sbjct: 1   MVGTDNSQLGFGHFAWQLRNHSSMNASVEIKQQESLRTHINPGNCIFSTCVGMQGLAIPP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHYAG-PSGMPVSKSTNGSY 120
           PLP+FKVE  NVV  S QCLSPHFQNSL  P P QKE+ESLHYAG PS MP+S S NGSY
Sbjct: 61  PLPNFKVELLNVVQRSNQCLSPHFQNSLSIPFPSQKEEESLHYAGPPSEMPLSISNNGSY 120

Query: 121 SKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLL-EEWIAGGIDSVKYFPNTLS 180
            KGFLIFDQSGNQKRLMYAPM PP++  STVA+N  PG L EE + G I+SVKYFP+TL 
Sbjct: 121 PKGFLIFDQSGNQKRLMYAPMSPPVH--STVADNKRPGWLEEEGVVGDINSVKYFPSTLF 180

Query: 181 NENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQE 240
           NENY+A  ESEMHENTEEIDALLYSD DGTG SSDDEVTSTGHSPEMINEHYEK E+CQE
Sbjct: 181 NENYVAAGESEMHENTEEIDALLYSDYDGTGCSSDDEVTSTGHSPEMINEHYEKNERCQE 240

Query: 241 TTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTD 300
           TTT+VASSDGPRKRQRVHDG S+KS  IATV+  RVELQKFA+DAES CGMVHEEEAGTD
Sbjct: 241 TTTKVASSDGPRKRQRVHDGESVKSPRIATVSRARVELQKFANDAESGCGMVHEEEAGTD 300

Query: 301 VDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           VDFC S CKKD+IKETLRVLE  VPGAKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 VDFCNSFCKKDKIKETLRVLENFVPGAKGKDPLLVIDEAIDYLKSLKHEASVL 351

BLAST of MS012327 vs. ExPASy TrEMBL
Match: A0A6J1FRS4 (transcription factor SAC51-like OS=Cucurbita moschata OX=3662 GN=LOC111447860 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.4e-150
Identity = 277/352 (78.69%), Postives = 301/352 (85.51%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCIGMQGFAI-P 60
           MVG DNSQL F HFAWQL NH+SMN S+EIKQQESL  +INPGNC+FSTC+GMQG AI P
Sbjct: 1   MVGTDNSQLGFGHFAWQLRNHSSMNASVEIKQQESLRTHINPGNCIFSTCVGMQGLAIPP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHYAG-PSGMPVSKSTNGSY 120
           PL +FKVE  N V  S QCLSPHFQNSL  P P QKE+ESLHYAG PS MP+S S NGSY
Sbjct: 61  PLRNFKVELLNAVQRSNQCLSPHFQNSLSIPFPSQKEEESLHYAGPPSEMPLSISNNGSY 120

Query: 121 SKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSN 180
            KGFLIFDQSGNQKRLMYAPM PP++  STVA+N  PG +EE + G I+SVKYFP+TL N
Sbjct: 121 PKGFLIFDQSGNQKRLMYAPMSPPVH--STVADNKRPGWVEEGVVGDINSVKYFPSTLFN 180

Query: 181 ENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQET 240
           ENY+A  ESEMHENTEEIDALLYSD DGTG SSDDEVTSTGHSPEMINEHYEK+E+CQET
Sbjct: 181 ENYVAAGESEMHENTEEIDALLYSDYDGTGCSSDDEVTSTGHSPEMINEHYEKDERCQET 240

Query: 241 TTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDV 300
           TT+VASSD PRKRQRVHDG S+KS  IATV+  RVELQKFA+DAES CGMVHEEEAGT+V
Sbjct: 241 TTKVASSDCPRKRQRVHDGESVKSPRIATVSRARVELQKFANDAESGCGMVHEEEAGTNV 300

Query: 301 DFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
           DFC S CKKDRIKETLRVLE  VPGAKGKDPLLVIDEAI+YLKSLKHEAS L
Sbjct: 301 DFCNSFCKKDRIKETLRVLENFVPGAKGKDPLLVIDEAIDYLKSLKHEASVL 350

BLAST of MS012327 vs. ExPASy TrEMBL
Match: B7SHL0 (Putative transcription factor OS=Cucumis sativus OX=3659 GN=Csa_4G113170 PE=2 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.2e-143
Identity = 273/349 (78.22%), Postives = 291/349 (83.38%), Query Frame = 0

Query: 1   MVGADNSQLDFEHFAWQLHNHNSMNTSIEIKQQESLPPYINPGNCLFSTCIG-MQGFAIP 60
           MVG DNS+LDFEHFAWQLHN+NSMN SIE KQQES    IN  NC+FS C+G MQ FAIP
Sbjct: 1   MVGTDNSRLDFEHFAWQLHNYNSMNASIETKQQESCQTSINHENCIFSKCMGRMQRFAIP 60

Query: 61  PLPSFKVEQTNVVHGSRQCLSPHFQNSLGTPSPCQKEKESLHY--AGPSGMPVSKSTNGS 120
           PLPSF+VEQ NV+ GSR CLSPHFQNS GT    Q EKES+HY  AGPSGMPVSKS NGS
Sbjct: 61  PLPSFEVEQLNVIQGSRHCLSPHFQNSRGTFISYQNEKESMHYAHAGPSGMPVSKSNNGS 120

Query: 121 YSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEE-WIAGGIDSVKYFPNTL 180
           Y KGFLIFDQSGNQKRLMYAPMC P+YFPS V EN   G LEE      I+SVKY PNTL
Sbjct: 121 YPKGFLIFDQSGNQKRLMYAPMC-PVYFPSIVTENKCCGWLEEKGAVRDINSVKYSPNTL 180

Query: 181 SNENYIA-GEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQC 240
           SNENY+A GE SEMHENTEEIDALLYSD DGTG SSDDEVTSTGHSPEMINEH EKEEQC
Sbjct: 181 SNENYVADGESSEMHENTEEIDALLYSDYDGTGCSSDDEVTSTGHSPEMINEHCEKEEQC 240

Query: 241 QETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAG 300
           QETTTEVASSD PRKRQR+HDGG IKSLPIAT +  RVE Q +A+DAESSCGMVH+EEAG
Sbjct: 241 QETTTEVASSDVPRKRQRLHDGGYIKSLPIATGSCARVESQNYANDAESSCGMVHKEEAG 300

Query: 301 TDVDFCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLK 345
            D+DFC  SCKKDRI+ETLRVLE LVPGAKGKDPLLVIDEAI Y + LK
Sbjct: 301 ADIDFCYCSCKKDRIEETLRVLESLVPGAKGKDPLLVIDEAINYFEVLK 348

BLAST of MS012327 vs. TAIR 10
Match: AT5G09460.1 (sequence-specific DNA binding transcription factors;transcription regulators )

HSP 1 Score: 136.7 bits (343), Expect = 3.5e-32
Identity = 116/345 (33.62%), Postives = 170/345 (49.28%), Query Frame = 0

Query: 28  IEIKQQESLPPYINPGNCLFSTCIGMQGFAIPPLPSFKVEQTNVVHGSRQCLSPHFQNSL 87
           ++ KQQ+ LP  +NP  C+         +  P +P  ++ +        + L P FQ  L
Sbjct: 3   LDTKQQKWLPLGLNPQACVQDKA---TEYFRPGIPFPELGKVYAAEHQFRYLQPPFQALL 62

Query: 88  G--TPSPCQKEKESLHYAGPSGMPVSKSTNGSYSKGFLIFDQSGNQKRLMYAPMCPPLYF 147
                  C K+   L+    +G     +   S  K F++FDQSG Q RL+      PL F
Sbjct: 63  SRYDQQSCGKQVSCLNGRSSNGAAPEGALKSS-RKRFIVFDQSGEQTRLLQCGF--PLRF 122

Query: 148 PSTVAENHGPGLLEEWIAGGIDSVKYF-------PNTLSNENYIAGEE-SEMHENTEEID 207
           PS++    G       I G +   K F          L +E++  GEE SEMHE+TEEI+
Sbjct: 123 PSSMDAERGN------ILGALHPEKGFSKDHAIQEKILQHEDHENGEEDSEMHEDTEEIN 182

Query: 208 ALLYS-DDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETTTEV----ASSDGP-RKR 267
           ALLYS DDD   + SDDEV STGHSP  +     +++ C  TT E+    ++ DGP  KR
Sbjct: 183 ALLYSDDDDNDDWESDDEVMSTGHSPFTV-----EQQACNITTEELDETESTVDGPLLKR 242

Query: 268 QRVHDGGSIKSLP-------IATVAGERVELQKFASDAESSCGMVHEEEAGTDVDFCTSS 327
           Q++ D     S P       +  ++ E +     +S  E+  G+  E+            
Sbjct: 243 QKLLDHSYRDSSPSLVGTTKVKGLSDENLPESNISSKQETGSGLSDEQS----------- 302

Query: 328 CKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEAST 350
            +KD+I   LR+LE +VPGAKGK+ LL++DEAI+YLK LK   ++
Sbjct: 303 -RKDKIHTALRILESVVPGAKGKEALLLLDEAIDYLKLLKQSLNS 318

BLAST of MS012327 vs. TAIR 10
Match: AT5G64340.1 (sequence-specific DNA binding transcription factors;transcription regulators )

HSP 1 Score: 132.1 bits (331), Expect = 8.6e-31
Identity = 118/333 (35.44%), Postives = 159/333 (47.75%), Query Frame = 0

Query: 31  KQQESLPPYINPGNCLFSTCIGMQGFAIPPLPSFKVEQTNVVHGSRQCLS-PHFQNSLGT 90
           K+Q  LP  ++P  C F   +G     +P +P  ++ +        +CL  P FQ+ L  
Sbjct: 5   KRQRDLPLGLSPQAC-FKDIVGRS--VLPRIPLPELGKLYAAKLQARCLQPPPFQSLL-- 64

Query: 91  PSPCQKEKESL-----------HYAGPSGMPVSKSTNGSYSKGFLIFDQSGNQKRLMYAP 150
              C  +KES              A  +          S  K  LIFDQSG+Q RL+  P
Sbjct: 65  ---CSHDKESYGKRFSRSDMRSWCAAATTTTTPLGALESSQKRLLIFDQSGDQTRLLQCP 124

Query: 151 MCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSNENYIAGEESEMHENTEEIDA 210
              PL FPS  A    P  L E    GI+  K F       +   G ESEMHE+TEEI+A
Sbjct: 125 F--PLRFPSHAAAE--PVKLSE--LQGIE--KAFKEDGEEFHKSDGTESEMHEDTEEINA 184

Query: 211 LLYSDDD-GTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETTTEVASSDGPRKRQ----R 270
           LLYSDDD      SDDEV STGHSP      Y  E  C +   E+   DGP KRQ    +
Sbjct: 185 LLYSDDDYDDDCESDDEVMSTGHSP------YPNEGVCNK--RELEEIDGPCKRQKLLDK 244

Query: 271 VHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDVDFCTSSCKKDRIKET 330
           V++   + SL + T +  ++    F  D +         +  T         KKD+I+  
Sbjct: 245 VNNISDLSSL-VGTESSTQLNGSSFLKDKKLPESKTISTKEDTGSGLSNEQSKKDKIRTA 304

Query: 331 LRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHE 347
           L++LE +VPGAKG + LL++DEAI+YLK LK +
Sbjct: 305 LKILESVVPGAKGNEALLLLDEAIDYLKLLKRD 312

BLAST of MS012327 vs. TAIR 10
Match: AT5G50010.1 (sequence-specific DNA binding transcription factors;transcription regulators )

HSP 1 Score: 84.0 bits (206), Expect = 2.7e-16
Identity = 77/240 (32.08%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 110 VSKSTNGSYSKGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSV 169
           +SK+      K FL+FDQSG+Q  L+ A      +   T+ ++  P + EE      D  
Sbjct: 97  ISKAEEQCSQKRFLVFDQSGDQTTLLLASDIRKSF--ETLKQHACPDMKEELQRSNKD-- 156

Query: 170 KYFPNTLSNENYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHY 229
                           E ++ E++EE++ALLYS+D+    S +DEVTS  HSP ++    
Sbjct: 157 -----LFVCHGMQGNSEPDLKEDSEELNALLYSEDESGYCSEEDEVTSADHSPSIVVSGR 216

Query: 230 EKEEQCQETTTEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMV 289
           E ++      T + S   P          + K   I   + E +       DAESSCG  
Sbjct: 217 EDQK------TFLGSYGQPL---------NAKKRKILETSNESMR------DAESSCGSC 276

Query: 290 HEEEAGTDVDFCTSSCK--KDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEA 348
                        SS K  +++I ET+ +L  +VPG +  DP+LVID AI+YLKSLK EA
Sbjct: 277 DNTRISFLKRSKLSSNKIGEEKIFETVSLLRSVVPGEELVDPILVIDRAIDYLKSLKMEA 306

BLAST of MS012327 vs. TAIR 10
Match: AT1G29950.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.7e-07
Identity = 58/231 (25.11%), Postives = 91/231 (39.39%), Query Frame = 0

Query: 120 KGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSNE 179
           K F+IFDQ+ ++ ++MY P        +    N       E++ G   +           
Sbjct: 53  KNFVIFDQTYDRSQVMYHPELTHKLMNTPSLNNLASTFQNEYVGGSYGNY---------G 112

Query: 180 NYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETT 239
           NY     S   E+  EIDALL +D+D      ++     G S E+        +    T 
Sbjct: 113 NYEQEVSSSYQEDPNEIDALLSADEDYEENDDNEGEEDGGDSEEVSTARTSSRDYGNTTA 172

Query: 240 TEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDVD 299
               SS G       ++  S K              Q  +  A SS    + +  G    
Sbjct: 173 ESCCSSYGYNNN---NNNNSRK--------------QSLSGSASSS----NNDGKG---- 232

Query: 300 FCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
                  + ++K+ + VL R+VPG +  +   V+DEA++YLKSLK EA  L
Sbjct: 233 -------RKKMKKMMGVLRRIVPGGEQMNTACVLDEAVQYLKSLKIEAQKL 242

BLAST of MS012327 vs. TAIR 10
Match: AT1G29950.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.7e-07
Identity = 58/231 (25.11%), Postives = 91/231 (39.39%), Query Frame = 0

Query: 120 KGFLIFDQSGNQKRLMYAPMCPPLYFPSTVAENHGPGLLEEWIAGGIDSVKYFPNTLSNE 179
           K F+IFDQ+ ++ ++MY P        +    N       E++ G   +           
Sbjct: 53  KNFVIFDQTYDRSQVMYHPELTHKLMNTPSLNNLASTFQNEYVGGSYGNY---------G 112

Query: 180 NYIAGEESEMHENTEEIDALLYSDDDGTGYSSDDEVTSTGHSPEMINEHYEKEEQCQETT 239
           NY     S   E+  EIDALL +D+D      ++     G S E+        +    T 
Sbjct: 113 NYEQEVSSSYQEDPNEIDALLSADEDYEENDDNEGEEDGGDSEEVSTARTSSRDYGNTTA 172

Query: 240 TEVASSDGPRKRQRVHDGGSIKSLPIATVAGERVELQKFASDAESSCGMVHEEEAGTDVD 299
               SS G       ++  S K              Q  +  A SS    + +  G    
Sbjct: 173 ESCCSSYGYNNN---NNNNSRK--------------QSLSGSASSS----NNDGKG---- 232

Query: 300 FCTSSCKKDRIKETLRVLERLVPGAKGKDPLLVIDEAIEYLKSLKHEASTL 351
                  + ++K+ + VL R+VPG +  +   V+DEA++YLKSLK EA  L
Sbjct: 233 -------RKKMKKMMGVLRRIVPGGEQMNTACVLDEAVQYLKSLKIEAQKL 242

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022984572.11.1e-15581.07transcription factor bHLH143-like [Cucurbita maxima][more]
XP_022922510.13.1e-15581.07transcription factor bHLH143-like [Cucurbita moschata][more]
KAG6576874.11.2e-15480.79Transcription factor basic helix-loop-helix 143, partial [Cucurbita argyrosperma... [more]
XP_023552238.16.4e-15380.23transcription factor bHLH143-like [Cucurbita pepo subsp. pepo][more]
XP_038903965.18.4e-15380.00transcription factor bHLH143-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FY694.9e-3133.62Transcription factor bHLH143 OS=Arabidopsis thaliana OX=3702 GN=BHLH143 PE=1 SV=... [more]
Q9FMF41.2e-2935.44Transcription factor SAC51 OS=Arabidopsis thaliana OX=3702 GN=SAC51 PE=2 SV=1[more]
Q9FGB03.8e-1532.08Transcription factor bHLH145 OS=Arabidopsis thaliana OX=3702 GN=BHLH145 PE=1 SV=... [more]
Q9ASX91.2e-0525.11Transcription factor bHLH144 OS=Arabidopsis thaliana OX=3702 GN=BHLH144 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1J2I75.1e-15681.07transcription factor bHLH143-like OS=Cucurbita maxima OX=3661 GN=LOC111482828 PE... [more]
A0A6J1E8Z41.5e-15581.07transcription factor bHLH143-like OS=Cucurbita moschata OX=3662 GN=LOC111430490 ... [more]
A0A6J1KRG34.5e-15279.60transcription factor bHLH143-like OS=Cucurbita maxima OX=3661 GN=LOC111497979 PE... [more]
A0A6J1FRS41.4e-15078.69transcription factor SAC51-like OS=Cucurbita moschata OX=3662 GN=LOC111447860 PE... [more]
B7SHL02.2e-14378.22Putative transcription factor OS=Cucumis sativus OX=3659 GN=Csa_4G113170 PE=2 SV... [more]
Match NameE-valueIdentityDescription
AT5G09460.13.5e-3233.62sequence-specific DNA binding transcription factors;transcription regulators [more]
AT5G64340.18.6e-3135.44sequence-specific DNA binding transcription factors;transcription regulators [more]
AT5G50010.12.7e-1632.08sequence-specific DNA binding transcription factors;transcription regulators [more]
AT1G29950.18.7e-0725.11basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G29950.28.7e-0725.11basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 333..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 231..256
NoneNo IPR availablePANTHERPTHR36066:SF8TRANSCRIPTION FACTOR SAC51coord: 8..350
NoneNo IPR availableCDDcd18917bHLH_AtSAC51_likecoord: 306..350
e-value: 1.03786E-15
score: 68.5936
IPR037546Transcription factor SAC51-likePANTHERPTHR36066TRANSCRIPTION FACTOR BHLH145coord: 8..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012327.1MS012327.1mRNA