MS012307 (gene) Bitter gourd (TR) v1

BLAST of MS012307 vs. NCBI nr
Match: XP_022150014.1 (subtilisin-like protease SBT1.1 [Momordica charantia] >XP_022150015.1 subtilisin-like protease SBT1.1 [Momordica charantia] >XP_022150016.1 subtilisin-like protease SBT1.1 [Momordica charantia])

HSP 1 Score: 1472.6 bits (3811), Expect = 0.0e+00
Identity = 751/768 (97.79%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDT KMPATDPEQWYTSMIHSVNELPSL+
Sbjct: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTTKMPATDPEQWYTSMIHSVNELPSLN 60

Query: 61  TDEGEASNTAEILYVYKTALS---GNILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNF 120
           TDEGEASNTAE+LYVYKTALS     +    LDAISKIRGFVAAIPDELLQLHTTHSSNF
Sbjct: 61  TDEGEASNTAEVLYVYKTALSXFAAKLTTRKLDAISKIRGFVAAIPDELLQLHTTHSSNF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS
Sbjct: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA
Sbjct: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI
Sbjct: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ
Sbjct: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLYSGKNTNQLPLVYNKTAG QEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV
Sbjct: 361 GSSLYSGKNTNQLPLVYNKTAGPQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT
Sbjct: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           RYGSRAPRVAAFSSRGPNPIQRTI+KPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI
Sbjct: 481 RYGSRAPRVAAFSSRGPNPIQRTIMKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
           AFAFGSGHVDPEKASDPGLVYDI PRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK
Sbjct: 601 AFAFGSGHVDPEKASDPGLVYDITPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
           PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF
Sbjct: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           KRLGEKLSYKVSFIS GKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ
Sbjct: 721 KRLGEKLSYKVSFISLGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 768

BLAST of MS012307 vs. NCBI nr
Match: XP_023544740.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 582/768 (75.78%), Postives = 665/768 (86.59%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REV + LSI +ATS+  AAVDQ+++IIHMDT KM A  PEQWYT++I S+NE+ SL 
Sbjct: 1   MGFREVCLFLSIFLATST--AAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLH 60

Query: 61  TDEGEASNTAEILYVYKTALSGNILNID---LDAISKIRGFVAAIPDELLQLHTTHSSNF 120
            D+ EASN A+ILYVYKTA+SG    +    L ++SK  GF+AA P+ELLQLHTTHS  F
Sbjct: 61  -DQQEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGL+  HGLWNSS LASDI++G+LDTGIWPEH+SF DKG+P VP++WKG C+ G KFSPS
Sbjct: 121 LGLQRQHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQPGQKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGA A++KGYE+IVGRLN T T+RSPRDSDGHGTHTASTAAGN+V KA F++QA
Sbjct: 181 NCNRKLIGAMAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MG A GMRFTSRIAAYKVCW  GCANADILAAIDRAVADGVDVLSLSLGG A+ FY+D I
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           +IA FGAVRNGVFVSCSAGNSGPS STVSN+APWIMTVAASYTDRTFP TVKLG+GQVF+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFE 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLY+G N  QLPLVYN TAG QEAN+CTPGSL+P+MVKGKIVVCERGT SRTAKGEQV
Sbjct: 361 GSSLYTGNNIGQLPLVYNNTAGGQEANVCTPGSLVPSMVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGA MILINT+LEGEELFADPHVLPA +LGASAG+AI ++I+SSK QPK  I F GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIGFEGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           ++G+RAPRVAAFSSRGP+ I   +IKPDVTAPGVNILAAWP I SPSELESDKRRVLFN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNV 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYT DN+M  ISDVGS SG+PAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
            FAFGSGHVDPEKASDPGL+YDI P+DYL+Y CSLNY S+QI L+SR NF+CPS+    +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLDYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
           PG+LNYPSFSV M +KKA NVSVTLKRTVTNVG PR DY+VKI +PKG+ I VKP+KLSF
Sbjct: 661 PGNLNYPSFSVFM-KKKAKNVSVTLKRTVTNVGGPRSDYSVKIKNPKGIVISVKPEKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           +R G+KLSY+VSF++ GK +     SFGSLVW+SGKY+VRSP+AVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGKYAVRSPIAVTWK 764

BLAST of MS012307 vs. NCBI nr
Match: XP_022990941.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022990948.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 582/768 (75.78%), Postives = 666/768 (86.72%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REV + LSI +ATS  AAAVDQ+++IIHMDT KM A  PEQWYT++I S+N++ SLD
Sbjct: 1   MGFREVCLFLSIFLATS--AAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLD 60

Query: 61  TDEGEASNTAEILYVYKTALSGNILNID---LDAISKIRGFVAAIPDELLQLHTTHSSNF 120
            D+ EAS+ A+ILYVYKTA+SG    +    L ++SK  GF+AA P+ELLQLHTTHS  F
Sbjct: 61  -DQEEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGL+ +HGLWNSS LASDI++G+LDTGIWPEH+SF DKG+P VP++WKG C+ G KFSPS
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGA A++KGYE+IVGRLN T T+RSPRDSDGHGTHTASTAAGN+V KA F++QA
Sbjct: 181 NCNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MG A GMRFTSRIAAYKVCW  GCANADILAAIDRAVADGVDVLSLSLGG A+ FY+D I
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           +IA FGAVRNGVFVSCSAGNSGPS STVSN+APWIMTVAASYTDRTFP TVKLG+GQVF+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFE 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLYSG +  QLPLVYN TAG +EAN+CT GSL+P++VKGKIVVCERGT SRTAKGEQV
Sbjct: 361 GSSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGA MILINT+LEGEELFADPHVLPA +LGASAG+AI ++I+SSK QPK  I F GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           ++G+RAPRVAAFSSRGP+ I   +IKPDVTAPGVNILAAWP I SPSELESDKRRVLFNI
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNI 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYT DN+M  ISDVGS SG+PAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
            FAFGSGHVDPEKASDPGL+YDI P+DYLNY CSLNY S+QI L+SR NF+CPS+    +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
           PG+LNYPSFSV M +KKA NVSVTLKRTVTNVG PR DYTVKI +PKG+ I V+P+KLSF
Sbjct: 661 PGNLNYPSFSVFM-KKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           +R G+KLSY+VSF++ GK +  G  SFGSLVW+SGKY VRSP+AVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of MS012307 vs. NCBI nr
Match: XP_038893069.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038893070.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 584/770 (75.84%), Postives = 660/770 (85.71%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REVW+ LSIM+A   A AAVDQ+T+IIHMDT KM  T+PEQWYT+MI S+NELPSLD
Sbjct: 1   MGFREVWMFLSIMLAI--ATAAVDQQTYIIHMDTTKMTTTNPEQWYTAMIDSLNELPSLD 60

Query: 61  TD-EGEASNTAEILYVYKTALSGNILNID---LDAISKIRGFVAAIPDELLQLHTTHSSN 120
            + + EAS+TAEILYVYKTALSG    +    LD++SKI GF+AA P+ELLQLHTTHS  
Sbjct: 61  DENKEEASDTAEILYVYKTALSGFAAKLSRKKLDSLSKIPGFLAATPNELLQLHTTHSPQ 120

Query: 121 FLGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSP 180
           FLGLE DHGLWNSS LASDII+G+LDTGIWPEH+SF DKG+  VP +WKG+C+ GPKFSP
Sbjct: 121 FLGLERDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPSKWKGICQAGPKFSP 180

Query: 181 SNCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQ 240
           SNCN+KLIGA+A++KGYE+IVG LN+T T+RSPRDSDGHGTHTASTAAG++V KA F++Q
Sbjct: 181 SNCNKKLIGASAYIKGYEAIVGTLNETGTFRSPRDSDGHGTHTASTAAGSMVNKASFFNQ 240

Query: 241 AMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDG 300
            MG A GM +TSRIAAYKVCWP GCANADILAA+D AVADGVDVLSLSLGG A  FYRD 
Sbjct: 241 GMGVATGMMYTSRIAAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDN 300

Query: 301 ISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVF 360
           I+IAAFGA++NGVFVSCSAGNSGP  STV N APWIMTVAASYTDRTFPTTVKLG+GQVF
Sbjct: 301 IAIAAFGAIQNGVFVSCSAGNSGPFISTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVF 360

Query: 361 QGSSLYSGKNTNQLPLVYNKTAG-RQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGE 420
           +GSSLY GKN N LPLVYN TAG  QE N CT GSL P MVKGKIV+CERG+ SRT KGE
Sbjct: 361 EGSSLYYGKNINYLPLVYNNTAGDGQETNFCTAGSLDPTMVKGKIVLCERGSNSRTDKGE 420

Query: 421 QVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFG 480
           QVKLAGGA MILINT+ EGEELFADPHVLPAT+LGASAGKAI  +IASSK Q K S+ F 
Sbjct: 421 QVKLAGGAGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKSQAKASMAFE 480

Query: 481 GTRYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLF 540
           GT+YGSRAPRVAAFSSRGP+ +   ++KPDVTAPGVNILAAWP I+SPSEL+SDKRRVLF
Sbjct: 481 GTKYGSRAPRVAAFSSRGPSFVGPDVMKPDVTAPGVNILAAWPPIVSPSELDSDKRRVLF 540

Query: 541 NIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRP 600
           NIISGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAY TDNKM L+SDVG  SG P
Sbjct: 541 NIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKMNLVSDVGHPSGGP 600

Query: 601 ANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAF 660
           A+ FAFGSGHVDPEKASDPGLVYDI P+DY+NYLCSL Y S+QIAL+SR NF+C S+   
Sbjct: 601 ADPFAFGSGHVDPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTL 660

Query: 661 LKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKL 720
           L+P DLNYPSFSV M +KKA NVS+T KRTVTNVG PR DYTVKIN+PKG+ I VKP+KL
Sbjct: 661 LQPRDLNYPSFSVFM-KKKAKNVSITSKRTVTNVGIPRSDYTVKINNPKGIRITVKPEKL 720

Query: 721 SFKRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           SF  LGEKLS++VSF++ G  ++    SFG LVWLSGKY+VRSP+AVTWQ
Sbjct: 721 SFGSLGEKLSFQVSFVALGGKEALSKFSFGDLVWLSGKYAVRSPIAVTWQ 767

BLAST of MS012307 vs. NCBI nr
Match: XP_022941552.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022941553.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 578/768 (75.26%), Postives = 664/768 (86.46%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REV + LSI +ATS+  AAVDQ+++IIHMDT KM A  PEQWYT++I S+NE+ SL+
Sbjct: 1   MGFREVCLFLSIFLATST--AAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLE 60

Query: 61  TDEGEASNTAEILYVYKTALSGNILNID---LDAISKIRGFVAAIPDELLQLHTTHSSNF 120
            D+ EASN A+ILYVYKTA+SG    +    L ++SK  GF+AA P+ELLQLHTTHS  F
Sbjct: 61  -DQEEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGL+ +HGLWNSS LASDI++G+LDTGIWPEH+SF DKG+P VP++WKG C+ G KFSPS
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGA A++KGYE+IVGRLN T T+RSPRDSDGHGTHTASTAAGN+V KA F++QA
Sbjct: 181 NCNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MG A GMRFTSRIAAYKVCW  GCANADILAAIDRAVADGVDVLSLSLGG A+ FY+D I
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           +IA FGAVRNGVFVSCSAGNSGPS STVSN+APWIMTVAASYTDR+FP TVKLG+GQVF+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFE 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLYSG N  QLPLVYN TAG ++AN+CT GSL+P+MVKGKIVVCERGT SRTAKGEQV
Sbjct: 361 GSSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGA MILINT+LEGEELFADPHVLPA +LGASAG+AI ++I+SSK QPK  I F GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           ++G+RAPRVAAFSSRGP+ I   +IKPDVTAPGVNILAAWP I SPSE+ESDKRRVLFN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNV 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYT DN+M  ISDVGS SG+PAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
            FAFGSGHVDPEKASDPGL+YDI P+DYLNYLCSLNY S+QI L+SR NF+CPS+    +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
            G LNYPSFSV M +KKA NVSVTLKRTVTNVG PR DY+VKI +PKG+ I VKP+KLSF
Sbjct: 661 AGKLNYPSFSVFM-KKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           +R G+KLSY+VSF++ GK +     SFGSLVW+SG Y+VRSP+AVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of MS012307 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 5.4e-232
Identity = 418/746 (56.03%), Postives = 536/746 (71.85%), Query Frame = 0

Query: 25  QRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLDTDEGEASNTAEILYVYKTALSG-- 84
           ++T++IH  T     T  +   TS+ +S+ +  +++ D+    +  EI Y+Y+ A+SG  
Sbjct: 41  KQTYVIHTVT-----TSTKHIVTSLFNSL-QTENINDDD---FSLPEIHYIYENAMSGFS 100

Query: 85  -NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNFLGLELDHGLWNSSTLASDIIVGVL 144
             + +  LD +   +GF++A PDELL LHTT+S  FLGLE   GLWN ++L+SD+I+G++
Sbjct: 101 ATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLV 160

Query: 145 DTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPSNCNRKLIGAAAFLKGYESIVGRLN 204
           DTGI PEHVSF D  +  VP RW+G C++G  FS S CN+K+IGA+AF KGYESIVG++N
Sbjct: 161 DTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKIN 220

Query: 205 QTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQAMGAAAGMRFTSRIAAYKVCWPAGC 264
           +T  +RS RD+ GHGTHTASTAAG++V KA ++ QA G A+GMRFTSRIAAYK CW  GC
Sbjct: 221 ETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGC 280

Query: 265 ANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGISIAAFGAVRNGVFVSCSAGNSGPS 324
           A+ D++AAIDRA+ DGVDV+SLSLGG + PFY D I+IA FGA++  +FVSCSAGNSGP+
Sbjct: 281 ASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPT 340

Query: 325 PSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQGSSLYSGKNTNQLPLVYNKTAGRQ 384
            STVSN APW+MTVAASYTDRTFP  V++G+ +   GSSLY GK+   LPL +N+TAG +
Sbjct: 341 ASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEE 400

Query: 385 E-ANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQVKLAGGAAMILINTELEGEELFAD 444
             A  C   SL   +V+GKIV+C RG   RTAKGE+VK +GGAAM+L++TE EGEEL AD
Sbjct: 401 SGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLAD 460

Query: 445 PHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGTRYGSRAPRVAAFSSRGPNPIQRT 504
           PHVLPA SLG S GK +  ++A + +    S+ F GT YG+ AP VAAFSSRGP+     
Sbjct: 461 PHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGTAYGATAPMVAAFSSRGPSVAGPE 520

Query: 505 IIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKAAHK 564
           I KPD+ APG+NILA W    SPS L SD RRV FNIISGTSM+CPH+SG+AAL+K+ H 
Sbjct: 521 IAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHG 580

Query: 565 DWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGR-PANAFAFGSGHVDPEKASDPGLVYD 624
           DWSPA IKSA+MTTA  TDN+ R I D G+      A AFAFG+G+VDP +A DPGLVYD
Sbjct: 581 DWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYD 640

Query: 625 IMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLKPGDLNYPSFSVIMDQKKATNVS 684
               DYLNYLCSLNYTS +I L S  N++C S    L PGDLNYPSF+V +    A   +
Sbjct: 641 TSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNL-VNGANLKT 700

Query: 685 VTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSFKRLGEKLSYKVSFISSGKGKSF 744
           V  KRTVTNVGSP  +Y V +  PKGV + V+P+ L F++  E+LSY V++ +     S 
Sbjct: 701 VRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEASRNS- 760

Query: 745 GDSSFGSLVWLSGKYSVRSPVAVTWQ 766
             SSFG LVW+  KY+VRSP+AVTW+
Sbjct: 761 SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of MS012307 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 2.4e-192
Identity = 374/777 (48.13%), Postives = 486/777 (62.55%), Query Frame = 0

Query: 10  LSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPE--QWYTSMIHSVNELPSLDTDEGEAS 69
           L  + + SS+A++ +  T+I+H+D    P+  P    WYTS + S+   P          
Sbjct: 10  LLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------- 69

Query: 70  NTAEILYVYKTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNFLGLELDH 129
               I++ Y T   G    + + D   +      ++ IP+++  LHTT S  FLGL    
Sbjct: 70  --PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 129

Query: 130 --GLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPSNCNRK 189
             GL   S   SD+++GV+DTG+WPE  SF D+G+  VP +WKG C     F  S CNRK
Sbjct: 130 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 189

Query: 190 LIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQAMGAAA 249
           L+GA  F  GYE+  G++N+T  +RSPRDSDGHGTHTAS +AG  V+ A     A G AA
Sbjct: 190 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 249

Query: 250 GMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGISIAAF 309
           GM   +R+AAYKVCW +GC ++DILAA D AVADGVDV+SLS+GG   P+Y D I+I AF
Sbjct: 250 GMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAF 309

Query: 310 GAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQGSSLY 369
           GA+  G+FVS SAGN GP   TV+NVAPW+ TV A   DR FP  VKLG+G++  G S+Y
Sbjct: 310 GAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVY 369

Query: 370 SGKNTN---QLPLVYNKT---AGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQ 429
            G   +     PLVY  +        ++LC  GSL P +VKGKIV+C+RG  SR  KGE 
Sbjct: 370 GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 429

Query: 430 VKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIAS-----SKDQPKGS 489
           V+  GG  MI+ N   +GE L AD HVLPATS+GAS G  I+++I+      S   P  +
Sbjct: 430 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 489

Query: 490 ITFGGTRYGSR-APRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDK 549
           I F GTR G R AP VA+FS+RGPNP    I+KPDV APG+NILAAWP  I PS + SD 
Sbjct: 490 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 549

Query: 550 RRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGS 609
           RR  FNI+SGTSM+CPHVSGLAALLKAAH DWSPAAI+SAL+TTAYT DN    + D   
Sbjct: 550 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--E 609

Query: 610 TSGRPANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCP 669
           ++G  ++   +GSGHV P KA DPGLVYDI   DY+N+LC+ NYT + I  ++R    C 
Sbjct: 610 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 669

Query: 670 SRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIV 729
                   G+LNYPSFSV+  Q   + +S    RTVTNVG     Y +KI  P+G  + V
Sbjct: 670 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 729

Query: 730 KPQKLSFKRLGEKLSYKVSFISSGKGKSFGDSSF--GSLVWLSGKYSVRSPVAVTWQ 766
           +P+KLSF+R+G+KLS+ V   ++    S G ++   G +VW  GK +V SP+ VT Q
Sbjct: 730 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of MS012307 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 2.7e-191
Identity = 363/776 (46.78%), Postives = 496/776 (63.92%), Query Frame = 0

Query: 6   VWVVLSIMVATSSAAAAVD---QRTFIIHMD--TMKMPATDPEQWYTSMIHSVNELPSLD 65
           ++++LSI +    A        ++T++IHMD   M +P T+  QWY+S I+SV +  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  TDEGEASNTAEILYVYKTA---LSGNILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNF 125
             + E  N   ILY Y+TA   L+  +   + + + +  G VA IP+   +LHTT S  F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGLELDHG--LWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFS 185
           LGLE      +W       D++VGVLDTGIWPE  SF+D G+  VP  W+G CE G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 PSNCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYD 245
             NCNRK++GA  F +GYE+  G++++   Y+SPRD DGHGTHTA+T AG+ V  A  + 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRD 305
            A G A GM   +R+AAYKVCW  GC ++DIL+A+D+AVADGV VLS+SLGG  + + RD
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 GISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQV 365
            +SIA FGA+  GVFVSCSAGN GP P +++NV+PWI TV AS  DR FP TVK+G+ + 
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FQGSSLYSGK----NTNQLPLVY--NKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTIS 425
           F+G SLY G+       Q PLVY     +     + C  G+L    V GKIV+C+RG   
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RTAKGEQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPK 485
           R  KG+ VK AGG  M+L NT   GEEL AD H+LPA ++G   GK IK++  +SK +  
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KAT 491

Query: 486 GSITFGGTRYGSR-APRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELES 545
            S+   GTR G + +P VAAFSSRGPN +   I+KPD+ APGVNILAAW   ++PS L S
Sbjct: 492 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 551

Query: 546 DKRRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDV 605
           D RRV FNI+SGTSMSCPHVSG+AAL+K+ H DWSPAAIKSALMTTAY  DN  + ++D 
Sbjct: 552 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD- 611

Query: 606 GSTSGRPANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFS 665
            ++   P++ +  G+GH+DP +A+DPGLVYDI P++Y  +LC+ + + SQ+ + ++ +  
Sbjct: 612 -ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 671

Query: 666 CPSRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAI 725
                    PG+LNYP+ S +  +      ++TL+RTVTNVG     Y V ++  KG ++
Sbjct: 672 TCKHTLAKNPGNLNYPAISALFPENTHVK-AMTLRRTVTNVGPHISSYKVSVSPFKGASV 731

Query: 726 IVKPQKLSFKRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTW 765
            V+P+ L+F    +KLSY V+F +  + K      FG LVW S  + VRSPV +TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTFRTRFRMKR---PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MS012307 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 3.9e-190
Identity = 371/758 (48.94%), Postives = 492/758 (64.91%), Query Frame = 0

Query: 19  AAAAVDQRTFIIHMDTMKMPATDP--EQWYTSMIHSVNELPSLDTDEGEASNTAEILYVY 78
           ++++ DQ T+I+HM   +MP++      WY S + S+             S++AE+LY Y
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYTY 82

Query: 79  KTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNFLGL-ELDHGLWNSSTL 138
           + A+ G    +   + D++    G ++ +P+   +LHTT +  FLGL E    L+  +  
Sbjct: 83  ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGS 142

Query: 139 ASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPSNCNRKLIGAAAFLKG 198
            SD++VGVLDTG+WPE  S+ D+G   +P  WKG CE G  F+ S CNRKLIGA  F +G
Sbjct: 143 YSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARG 202

Query: 199 YESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQAMGAAAGMRFTSRIAA 258
           YES +G ++++   RSPRD DGHGTHT+STAAG++V  A     A G A GM   +R+A 
Sbjct: 203 YESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAV 262

Query: 259 YKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGISIAAFGAVRNGVFVS 318
           YKVCW  GC ++DILAAID+A+AD V+VLS+SLGG  + +YRDG++I AF A+  G+ VS
Sbjct: 263 YKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVS 322

Query: 319 CSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQGSSLYSGK--NTNQL 378
           CSAGN+GPS S++SNVAPWI TV A   DR FP    LG+G+ F G SL+ G+      L
Sbjct: 323 CSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLL 382

Query: 379 PLVY-NKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQVKLAGGAAMILIN 438
           P +Y    +     NLC  G+LIP  VKGKIV+C+RG  +R  KG+ VK AGG  MIL N
Sbjct: 383 PFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILAN 442

Query: 439 TELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGTRYGSR-APRVAA 498
           T   GEEL AD H+LPAT++G  AG  I+ ++ +  + P  SI+  GT  G + +P VAA
Sbjct: 443 TAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAA 502

Query: 499 FSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNIISGTSMSCPHV 558
           FSSRGPN I   I+KPD+ APGVNILAAW     P+ L SD RRV FNIISGTSMSCPHV
Sbjct: 503 FSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHV 562

Query: 559 SGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPANAFAFGSGHVDP 618
           SGLAALLK+ H +WSPAAI+SALMTTAY T    + + D+   +G+P+  F  G+GHV P
Sbjct: 563 SGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVSP 622

Query: 619 EKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSC-PSRGAFLKPGDLNYPSFS 678
             A++PGL+YD+   DYL +LC+LNYTS QI  +SR N++C PS+       DLNYPSF+
Sbjct: 623 TTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS--YSVADLNYPSFA 682

Query: 679 VIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINS-PKGVAIIVKPQKLSFKRLGEKLSY 738
           V +D   A   +    RTVT+VG   G Y+VK+ S   GV I V+P  L+FK   EK SY
Sbjct: 683 VNVDGVGAYKYT----RTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 742

Query: 739 KVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTW 765
            V+F +    K  G +SFGS+ W  GK+ V SPVA++W
Sbjct: 743 TVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MS012307 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 6.2e-188
Identity = 374/785 (47.64%), Postives = 497/785 (63.31%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVA--TSSAAAAVDQRTFIIHMDTMKMPA--TDPEQWYTSMIHSVNEL 60
           + L  ++ V  +++   + S++++    ++I+H+     P+  +    W+ S++ S   L
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS---L 63

Query: 61  PSLDTDEGEASNTAEILYVYKTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTH 120
           PS       +   A +LY Y  A+ G    +  I   A+ +    ++ IPD+  ++HTTH
Sbjct: 64  PS-------SPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTH 123

Query: 121 SSNFLGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPK 180
           +  FLG   + GLW++S    D+IVGVLDTGIWPEH SF D G+  +P  WKG CE GP 
Sbjct: 124 TPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPD 183

Query: 181 FSPSNCNRKLIGAAAFLKGYESIVGRLNQTAT--YRSPRDSDGHGTHTASTAAGNLVYKA 240
           F  S+CNRKLIGA AF +GY +      + A    RSPRD++GHGTHTASTAAG++V  A
Sbjct: 184 FPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANA 243

Query: 241 GFYDQAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLG--GRA 300
             Y  A G A GM   +RIAAYK+CW  GC ++DILAA+D+AVADGV V+SLS+G  G A
Sbjct: 244 SLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSA 303

Query: 301 APFYRDGISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVK 360
             ++ D I+I AFGA R+G+ VSCSAGNSGP+P T +N+APWI+TV AS  DR F     
Sbjct: 304 PEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAI 363

Query: 361 LGSGQVFQGSSLYSGKN--TNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGT 420
            G G+VF G+SLY+G++   +QL LVY+   G   + LC PG L  ++V+GKIV+C+RG 
Sbjct: 364 TGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGG 423

Query: 421 ISRTAKGEQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQ 480
            +R  KG  VKLAGGA MIL NT   GEEL AD H++PAT +GA AG  I+ +I +S D 
Sbjct: 424 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DS 483

Query: 481 PKGSITFGGTRYGSR--APRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSE 540
           P   I+F GT  G    +PRVAAFSSRGPN +   I+KPDV APGVNILA W  ++ P++
Sbjct: 484 PTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTD 543

Query: 541 LESDKRRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLI 600
           L+ D RRV FNIISGTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N    I
Sbjct: 544 LDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPI 603

Query: 601 SDVGSTSGRPANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRE 660
            D+   +G+ +N+F  G+GHVDP KA +PGLVYDI  ++Y+ +LC++ Y    I +  ++
Sbjct: 604 EDL--ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQD 663

Query: 661 N--FSCPSRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGS-PRGDYTVKINS 720
              +           GDLNYPSFSV+     +T   V  KR V NVGS     Y V + S
Sbjct: 664 PTLYDACETSKLRTAGDLNYPSFSVVF---ASTGEVVKYKRVVKNVGSNVDAVYEVGVKS 723

Query: 721 PKGVAIIVKPQKLSFKRLGEKLSYKVSFISSGKGKSFGD---SSFGSLVWLSGKYSVRSP 765
           P  V I V P KL+F +    L Y+V+F S   G   G      FGS+ W  G++ V+SP
Sbjct: 724 PANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSP 769

BLAST of MS012307 vs. ExPASy TrEMBL
Match: A0A6J1D8S5 (subtilisin-like protease SBT1.1 OS=Momordica charantia OX=3673 GN=LOC111018293 PE=3 SV=1)

HSP 1 Score: 1472.6 bits (3811), Expect = 0.0e+00
Identity = 751/768 (97.79%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDT KMPATDPEQWYTSMIHSVNELPSL+
Sbjct: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTTKMPATDPEQWYTSMIHSVNELPSLN 60

Query: 61  TDEGEASNTAEILYVYKTALS---GNILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNF 120
           TDEGEASNTAE+LYVYKTALS     +    LDAISKIRGFVAAIPDELLQLHTTHSSNF
Sbjct: 61  TDEGEASNTAEVLYVYKTALSXFAAKLTTRKLDAISKIRGFVAAIPDELLQLHTTHSSNF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS
Sbjct: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA
Sbjct: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI
Sbjct: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ
Sbjct: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLYSGKNTNQLPLVYNKTAG QEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV
Sbjct: 361 GSSLYSGKNTNQLPLVYNKTAGPQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT
Sbjct: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           RYGSRAPRVAAFSSRGPNPIQRTI+KPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI
Sbjct: 481 RYGSRAPRVAAFSSRGPNPIQRTIMKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
           AFAFGSGHVDPEKASDPGLVYDI PRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK
Sbjct: 601 AFAFGSGHVDPEKASDPGLVYDITPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
           PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF
Sbjct: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           KRLGEKLSYKVSFIS GKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ
Sbjct: 721 KRLGEKLSYKVSFISLGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 768

BLAST of MS012307 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 582/768 (75.78%), Postives = 666/768 (86.72%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REV + LSI +ATS  AAAVDQ+++IIHMDT KM A  PEQWYT++I S+N++ SLD
Sbjct: 1   MGFREVCLFLSIFLATS--AAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLD 60

Query: 61  TDEGEASNTAEILYVYKTALSGNILNID---LDAISKIRGFVAAIPDELLQLHTTHSSNF 120
            D+ EAS+ A+ILYVYKTA+SG    +    L ++SK  GF+AA P+ELLQLHTTHS  F
Sbjct: 61  -DQEEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGL+ +HGLWNSS LASDI++G+LDTGIWPEH+SF DKG+P VP++WKG C+ G KFSPS
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGA A++KGYE+IVGRLN T T+RSPRDSDGHGTHTASTAAGN+V KA F++QA
Sbjct: 181 NCNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MG A GMRFTSRIAAYKVCW  GCANADILAAIDRAVADGVDVLSLSLGG A+ FY+D I
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           +IA FGAVRNGVFVSCSAGNSGPS STVSN+APWIMTVAASYTDRTFP TVKLG+GQVF+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFE 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLYSG +  QLPLVYN TAG +EAN+CT GSL+P++VKGKIVVCERGT SRTAKGEQV
Sbjct: 361 GSSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGA MILINT+LEGEELFADPHVLPA +LGASAG+AI ++I+SSK QPK  I F GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           ++G+RAPRVAAFSSRGP+ I   +IKPDVTAPGVNILAAWP I SPSELESDKRRVLFNI
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNI 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYT DN+M  ISDVGS SG+PAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
            FAFGSGHVDPEKASDPGL+YDI P+DYLNY CSLNY S+QI L+SR NF+CPS+    +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
           PG+LNYPSFSV M +KKA NVSVTLKRTVTNVG PR DYTVKI +PKG+ I V+P+KLSF
Sbjct: 661 PGNLNYPSFSVFM-KKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           +R G+KLSY+VSF++ GK +  G  SFGSLVW+SGKY VRSP+AVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of MS012307 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 578/768 (75.26%), Postives = 664/768 (86.46%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REV + LSI +ATS+  AAVDQ+++IIHMDT KM A  PEQWYT++I S+NE+ SL+
Sbjct: 1   MGFREVCLFLSIFLATST--AAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLE 60

Query: 61  TDEGEASNTAEILYVYKTALSGNILNID---LDAISKIRGFVAAIPDELLQLHTTHSSNF 120
            D+ EASN A+ILYVYKTA+SG    +    L ++SK  GF+AA P+ELLQLHTTHS  F
Sbjct: 61  -DQEEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGL+ +HGLWNSS LASDI++G+LDTGIWPEH+SF DKG+P VP++WKG C+ G KFSPS
Sbjct: 121 LGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCNRKLIGA A++KGYE+IVGRLN T T+RSPRDSDGHGTHTASTAAGN+V KA F++QA
Sbjct: 181 NCNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQA 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MG A GMRFTSRIAAYKVCW  GCANADILAAIDRAVADGVDVLSLSLGG A+ FY+D I
Sbjct: 241 MGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           +IA FGAVRNGVFVSCSAGNSGPS STVSN+APWIMTVAASYTDR+FP TVKLG+GQVF+
Sbjct: 301 AIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFE 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQV 420
           GSSLYSG N  QLPLVYN TAG ++AN+CT GSL+P+MVKGKIVVCERGT SRTAKGEQV
Sbjct: 361 GSSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQV 420

Query: 421 KLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGT 480
           KLAGGA MILINT+LEGEELFADPHVLPA +LGASAG+AI ++I+SSK QPK  I F GT
Sbjct: 421 KLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGT 480

Query: 481 RYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNI 540
           ++G+RAPRVAAFSSRGP+ I   +IKPDVTAPGVNILAAWP I SPSE+ESDKRRVLFN+
Sbjct: 481 KFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNV 540

Query: 541 ISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPAN 600
           ISGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYT DN+M  ISDVGS SG+PAN
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPAN 600

Query: 601 AFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLK 660
            FAFGSGHVDPEKASDPGL+YDI P+DYLNYLCSLNY S+QI L+SR NF+CPS+    +
Sbjct: 601 PFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQ 660

Query: 661 PGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSF 720
            G LNYPSFSV M +KKA NVSVTLKRTVTNVG PR DY+VKI +PKG+ I VKP+KLSF
Sbjct: 661 AGKLNYPSFSVFM-KKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSF 720

Query: 721 KRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           +R G+KLSY+VSF++ GK +     SFGSLVW+SG Y+VRSP+AVTW+
Sbjct: 721 RRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of MS012307 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 576/769 (74.90%), Postives = 654/769 (85.05%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLD 60
           MG REVW VLSIM+A SS  A VDQ+T+IIHMDT KM   +PEQWYT +I SVNEL SLD
Sbjct: 1   MGFREVW-VLSIMLAISS--AVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLD 60

Query: 61  TDEGEASNTAEILYVYKTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNF 120
            +E EASN AEILYVYKTALSG    + +  L ++SKI GF+AA P+ELLQLHTTHS  F
Sbjct: 61  DNE-EASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQF 120

Query: 121 LGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPS 180
           LGL+ DHGLWN S LASDII+G+LDTGIWPEH+SF DKG+  VP +WKG+C+ GP+FS S
Sbjct: 121 LGLQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSS 180

Query: 181 NCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQA 240
           NCN+KLIGA+A++KGYE+IVGRLN+T T+RSPRDSDGHGTHTASTAAG++V  A FY+Q 
Sbjct: 181 NCNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQG 240

Query: 241 MGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGI 300
           MG A+GMRFTSRI AYKVCWP GCANADILAA+D AVADGVDVLSLSLGG ++ FY+D I
Sbjct: 241 MGVASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNI 300

Query: 301 SIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQ 360
           +IAAFGA++ GVFVSCSAGNSGPSPSTV N APWIMTVAASYTDRTFPTTVKLG+GQVF+
Sbjct: 301 AIAAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFE 360

Query: 361 GSSLYSGKNTNQLPLVYNKTAG-RQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQ 420
           GSSLY GK+ N+LPLVYN TAG  QE N+C  GSL P+MVKGKIV+CERGTISRT KGEQ
Sbjct: 361 GSSLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQ 420

Query: 421 VKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGG 480
           VKLAGG  MILINT+ EGEELFADPHVLPAT+LGASAGKAI  +IASSK Q K SI F G
Sbjct: 421 VKLAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEG 480

Query: 481 TRYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFN 540
           T+YGS+APRVAAFSSRGP+ +   +IKPDVTAPGVNILAAWP I+SPSEL SD RRV+FN
Sbjct: 481 TKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFN 540

Query: 541 IISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPA 600
           IISGTSMSCPHVSGLAALLK+AH DWSPAAIKSALMTTAY TD+KM LISDVG  +G PA
Sbjct: 541 IISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPA 600

Query: 601 NAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFL 660
             F FGSGHVDPEKASDPGL+YDI P+DY+NYLCSL Y SSQIAL+SR N +C S+   +
Sbjct: 601 TPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVV 660

Query: 661 KPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLS 720
           KPGDLNYPSFSV M +KKA  VS+TLKRTVTNVG  R DYTVKIN+PKGV +IVKP+KLS
Sbjct: 661 KPGDLNYPSFSVFM-KKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLS 720

Query: 721 FKRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           F  LGE+LSYKVSF+S G  ++    SFGSLVW+SGKY+VRSP+ VTWQ
Sbjct: 721 FGSLGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of MS012307 vs. ExPASy TrEMBL
Match: A0A6J1JRF2 (subtilisin-like protease SBT1.1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487664 PE=3 SV=1)

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 576/771 (74.71%), Postives = 654/771 (84.82%), Query Frame = 0

Query: 1   MGLREVWVV-LSIMVATSSAAAAVDQRTFIIHMDTMKMPA-TDPEQWYTSMIHSVNELPS 60
           MG REVWV+ LSIM+A S  AAAVDQ+T+I+HMDT KM A T PEQWY++MIHSVN++ S
Sbjct: 13  MGFREVWVLCLSIMLAAS--AAAVDQQTYIVHMDTTKMVATTSPEQWYSAMIHSVNKISS 72

Query: 61  LDTDEGEASNTAEILYVYKTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTHSS 120
           L  DE EASN AEILYVYKTA+SG    +    L ++SKI GF+AA PDELLQLHTTHS 
Sbjct: 73  LHQDEEEASNAAEILYVYKTAISGFAAKLTTKKLHSLSKIPGFLAATPDELLQLHTTHSP 132

Query: 121 NFLGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFS 180
            FLGL  +HGLWNSS  ASDII+G++D+GIWPEH+SF DKG+P VP++WKG CE GPKFS
Sbjct: 133 QFLGLHREHGLWNSSNSASDIIIGLIDSGIWPEHISFQDKGLPPVPKKWKGACEAGPKFS 192

Query: 181 PSNCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYD 240
           PSNCN+KL+GA A++ GYE+IVGRLN T T+RSPRDS+GHGTHTASTAAGN+V KA  Y 
Sbjct: 193 PSNCNKKLVGARAYIDGYEAIVGRLNVTGTFRSPRDSNGHGTHTASTAAGNIVNKASLYS 252

Query: 241 QAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRD 300
           Q  G A G+ FTSRIAAYK CWP GCANADILAA+DRAVADGVD+LSLSLGG    FY+D
Sbjct: 253 QGKGVATGISFTSRIAAYKACWPMGCANADILAAMDRAVADGVDILSLSLGGGVTAFYKD 312

Query: 301 GISIAAFGAVRNGVFVSCSAGNSGP-SPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQ 360
            I+IAAFGAV+NGVFVSCSAGN G    S VSN APWIMTVAASYTDRTFPTTVKLG+GQ
Sbjct: 313 NIAIAAFGAVQNGVFVSCSAGNFGSLFRSAVSNAAPWIMTVAASYTDRTFPTTVKLGNGQ 372

Query: 361 VFQGSSLYSGKNTNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKG 420
           VF+GSSLYSG+NT +LPLVYN T G QEANLCTPGSL+PAMVKGKIVVC RG   R AKG
Sbjct: 373 VFEGSSLYSGQNTAELPLVYNNTVGGQEANLCTPGSLVPAMVKGKIVVCGRGKNPRVAKG 432

Query: 421 EQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITF 480
           EQVKLAGGA MILINT+L+GEELFA+PHVLPATSLGASA KA+  +IASSK+QP  SI F
Sbjct: 433 EQVKLAGGAGMILINTQLDGEELFAEPHVLPATSLGASATKAMLDYIASSKNQPTASIGF 492

Query: 481 GGTRYGSRAPRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVL 540
            GT+YGSRAP+VAAFSSRGP+ I   +IKPDVTAPGVNILAAWP I+SPSELE DKRRVL
Sbjct: 493 QGTKYGSRAPKVAAFSSRGPSLIGPDVIKPDVTAPGVNILAAWPPIVSPSELEFDKRRVL 552

Query: 541 FNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGR 600
           FNIISGTSMSCPHVSGLAAL+K+AH +WSPAAIKSALMTTAYTTDNKM  ISDV   +G 
Sbjct: 553 FNIISGTSMSCPHVSGLAALIKSAHNNWSPAAIKSALMTTAYTTDNKMSPISDVSHPNGE 612

Query: 601 PANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGA 660
           PA+ FA GSGHVDPEKASDPGLVYDI P DYLNYLCSLNY+S+QI LLS+ NFSCPS+  
Sbjct: 613 PADPFAIGSGHVDPEKASDPGLVYDIAPGDYLNYLCSLNYSSTQIGLLSKGNFSCPSKRE 672

Query: 661 FLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQK 720
            ++PGDLNYPSFSVIM   KA NVSVTLKRTVTNVG P  DY+V+IN+PKGV +IVKP++
Sbjct: 673 VVEPGDLNYPSFSVIM---KAKNVSVTLKRTVTNVGYPMSDYSVQINNPKGVEMIVKPKR 732

Query: 721 LSFKRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTWQ 766
           LSF+R GEKLSYKVSF++  + +  G+ SFGS+VW+SGKY VRSPVAVTWQ
Sbjct: 733 LSFERSGEKLSYKVSFVALEEAEDLGEFSFGSVVWVSGKYRVRSPVAVTWQ 778

BLAST of MS012307 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 805.4 bits (2079), Expect = 3.8e-233
Identity = 418/746 (56.03%), Postives = 536/746 (71.85%), Query Frame = 0

Query: 25  QRTFIIHMDTMKMPATDPEQWYTSMIHSVNELPSLDTDEGEASNTAEILYVYKTALSG-- 84
           ++T++IH  T     T  +   TS+ +S+ +  +++ D+    +  EI Y+Y+ A+SG  
Sbjct: 41  KQTYVIHTVT-----TSTKHIVTSLFNSL-QTENINDDD---FSLPEIHYIYENAMSGFS 100

Query: 85  -NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNFLGLELDHGLWNSSTLASDIIVGVL 144
             + +  LD +   +GF++A PDELL LHTT+S  FLGLE   GLWN ++L+SD+I+G++
Sbjct: 101 ATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLV 160

Query: 145 DTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPSNCNRKLIGAAAFLKGYESIVGRLN 204
           DTGI PEHVSF D  +  VP RW+G C++G  FS S CN+K+IGA+AF KGYESIVG++N
Sbjct: 161 DTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKIN 220

Query: 205 QTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQAMGAAAGMRFTSRIAAYKVCWPAGC 264
           +T  +RS RD+ GHGTHTASTAAG++V KA ++ QA G A+GMRFTSRIAAYK CW  GC
Sbjct: 221 ETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGC 280

Query: 265 ANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGISIAAFGAVRNGVFVSCSAGNSGPS 324
           A+ D++AAIDRA+ DGVDV+SLSLGG + PFY D I+IA FGA++  +FVSCSAGNSGP+
Sbjct: 281 ASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPT 340

Query: 325 PSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQGSSLYSGKNTNQLPLVYNKTAGRQ 384
            STVSN APW+MTVAASYTDRTFP  V++G+ +   GSSLY GK+   LPL +N+TAG +
Sbjct: 341 ASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEE 400

Query: 385 E-ANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQVKLAGGAAMILINTELEGEELFAD 444
             A  C   SL   +V+GKIV+C RG   RTAKGE+VK +GGAAM+L++TE EGEEL AD
Sbjct: 401 SGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLAD 460

Query: 445 PHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGTRYGSRAPRVAAFSSRGPNPIQRT 504
           PHVLPA SLG S GK +  ++A + +    S+ F GT YG+ AP VAAFSSRGP+     
Sbjct: 461 PHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGTAYGATAPMVAAFSSRGPSVAGPE 520

Query: 505 IIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKAAHK 564
           I KPD+ APG+NILA W    SPS L SD RRV FNIISGTSM+CPH+SG+AAL+K+ H 
Sbjct: 521 IAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHG 580

Query: 565 DWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGR-PANAFAFGSGHVDPEKASDPGLVYD 624
           DWSPA IKSA+MTTA  TDN+ R I D G+      A AFAFG+G+VDP +A DPGLVYD
Sbjct: 581 DWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYD 640

Query: 625 IMPRDYLNYLCSLNYTSSQIALLSRENFSCPSRGAFLKPGDLNYPSFSVIMDQKKATNVS 684
               DYLNYLCSLNYTS +I L S  N++C S    L PGDLNYPSF+V +    A   +
Sbjct: 641 TSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNL-VNGANLKT 700

Query: 685 VTLKRTVTNVGSPRGDYTVKINSPKGVAIIVKPQKLSFKRLGEKLSYKVSFISSGKGKSF 744
           V  KRTVTNVGSP  +Y V +  PKGV + V+P+ L F++  E+LSY V++ +     S 
Sbjct: 701 VRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDAEASRNS- 760

Query: 745 GDSSFGSLVWLSGKYSVRSPVAVTWQ 766
             SSFG LVW+  KY+VRSP+AVTW+
Sbjct: 761 SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of MS012307 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 673.7 bits (1737), Expect = 1.7e-193
Identity = 374/777 (48.13%), Postives = 486/777 (62.55%), Query Frame = 0

Query: 10  LSIMVATSSAAAAVDQRTFIIHMDTMKMPATDPE--QWYTSMIHSVNELPSLDTDEGEAS 69
           L  + + SS+A++ +  T+I+H+D    P+  P    WYTS + S+   P          
Sbjct: 10  LLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP---------- 69

Query: 70  NTAEILYVYKTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNFLGLELDH 129
               I++ Y T   G    + + D   +      ++ IP+++  LHTT S  FLGL    
Sbjct: 70  --PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTD 129

Query: 130 --GLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPSNCNRK 189
             GL   S   SD+++GV+DTG+WPE  SF D+G+  VP +WKG C     F  S CNRK
Sbjct: 130 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 189

Query: 190 LIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQAMGAAA 249
           L+GA  F  GYE+  G++N+T  +RSPRDSDGHGTHTAS +AG  V+ A     A G AA
Sbjct: 190 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 249

Query: 250 GMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGISIAAF 309
           GM   +R+AAYKVCW +GC ++DILAA D AVADGVDV+SLS+GG   P+Y D I+I AF
Sbjct: 250 GMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAF 309

Query: 310 GAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQGSSLY 369
           GA+  G+FVS SAGN GP   TV+NVAPW+ TV A   DR FP  VKLG+G++  G S+Y
Sbjct: 310 GAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVY 369

Query: 370 SGKNTN---QLPLVYNKT---AGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQ 429
            G   +     PLVY  +        ++LC  GSL P +VKGKIV+C+RG  SR  KGE 
Sbjct: 370 GGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 429

Query: 430 VKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIAS-----SKDQPKGS 489
           V+  GG  MI+ N   +GE L AD HVLPATS+GAS G  I+++I+      S   P  +
Sbjct: 430 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 489

Query: 490 ITFGGTRYGSR-APRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDK 549
           I F GTR G R AP VA+FS+RGPNP    I+KPDV APG+NILAAWP  I PS + SD 
Sbjct: 490 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 549

Query: 550 RRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGS 609
           RR  FNI+SGTSM+CPHVSGLAALLKAAH DWSPAAI+SAL+TTAYT DN    + D   
Sbjct: 550 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--E 609

Query: 610 TSGRPANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSCP 669
           ++G  ++   +GSGHV P KA DPGLVYDI   DY+N+LC+ NYT + I  ++R    C 
Sbjct: 610 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 669

Query: 670 SRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAIIV 729
                   G+LNYPSFSV+  Q   + +S    RTVTNVG     Y +KI  P+G  + V
Sbjct: 670 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 729

Query: 730 KPQKLSFKRLGEKLSYKVSFISSGKGKSFGDSSF--GSLVWLSGKYSVRSPVAVTWQ 766
           +P+KLSF+R+G+KLS+ V   ++    S G ++   G +VW  GK +V SP+ VT Q
Sbjct: 730 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of MS012307 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 670.2 bits (1728), Expect = 1.9e-192
Identity = 363/776 (46.78%), Postives = 496/776 (63.92%), Query Frame = 0

Query: 6   VWVVLSIMVATSSAAAAVD---QRTFIIHMD--TMKMPATDPEQWYTSMIHSVNELPSLD 65
           ++++LSI +    A        ++T++IHMD   M +P T+  QWY+S I+SV +  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  TDEGEASNTAEILYVYKTA---LSGNILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNF 125
             + E  N   ILY Y+TA   L+  +   + + + +  G VA IP+   +LHTT S  F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGLELDHG--LWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFS 185
           LGLE      +W       D++VGVLDTGIWPE  SF+D G+  VP  W+G CE G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 PSNCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYD 245
             NCNRK++GA  F +GYE+  G++++   Y+SPRD DGHGTHTA+T AG+ V  A  + 
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRD 305
            A G A GM   +R+AAYKVCW  GC ++DIL+A+D+AVADGV VLS+SLGG  + + RD
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 GISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQV 365
            +SIA FGA+  GVFVSCSAGN GP P +++NV+PWI TV AS  DR FP TVK+G+ + 
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FQGSSLYSGK----NTNQLPLVY--NKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTIS 425
           F+G SLY G+       Q PLVY     +     + C  G+L    V GKIV+C+RG   
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RTAKGEQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPK 485
           R  KG+ VK AGG  M+L NT   GEEL AD H+LPA ++G   GK IK++  +SK +  
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KAT 491

Query: 486 GSITFGGTRYGSR-APRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELES 545
            S+   GTR G + +P VAAFSSRGPN +   I+KPD+ APGVNILAAW   ++PS L S
Sbjct: 492 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 551

Query: 546 DKRRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDV 605
           D RRV FNI+SGTSMSCPHVSG+AAL+K+ H DWSPAAIKSALMTTAY  DN  + ++D 
Sbjct: 552 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTD- 611

Query: 606 GSTSGRPANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFS 665
            ++   P++ +  G+GH+DP +A+DPGLVYDI P++Y  +LC+ + + SQ+ + ++ +  
Sbjct: 612 -ASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 671

Query: 666 CPSRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSPKGVAI 725
                    PG+LNYP+ S +  +      ++TL+RTVTNVG     Y V ++  KG ++
Sbjct: 672 TCKHTLAKNPGNLNYPAISALFPENTHVK-AMTLRRTVTNVGPHISSYKVSVSPFKGASV 731

Query: 726 IVKPQKLSFKRLGEKLSYKVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTW 765
            V+P+ L+F    +KLSY V+F +  + K      FG LVW S  + VRSPV +TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTFRTRFRMKR---PEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MS012307 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 666.4 bits (1718), Expect = 2.8e-191
Identity = 371/758 (48.94%), Postives = 492/758 (64.91%), Query Frame = 0

Query: 19  AAAAVDQRTFIIHMDTMKMPATDP--EQWYTSMIHSVNELPSLDTDEGEASNTAEILYVY 78
           ++++ DQ T+I+HM   +MP++      WY S + S+             S++AE+LY Y
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-------------SDSAELLYTY 82

Query: 79  KTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTHSSNFLGL-ELDHGLWNSSTL 138
           + A+ G    +   + D++    G ++ +P+   +LHTT +  FLGL E    L+  +  
Sbjct: 83  ENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGS 142

Query: 139 ASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPKFSPSNCNRKLIGAAAFLKG 198
            SD++VGVLDTG+WPE  S+ D+G   +P  WKG CE G  F+ S CNRKLIGA  F +G
Sbjct: 143 YSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARG 202

Query: 199 YESIVGRLNQTATYRSPRDSDGHGTHTASTAAGNLVYKAGFYDQAMGAAAGMRFTSRIAA 258
           YES +G ++++   RSPRD DGHGTHT+STAAG++V  A     A G A GM   +R+A 
Sbjct: 203 YESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAV 262

Query: 259 YKVCWPAGCANADILAAIDRAVADGVDVLSLSLGGRAAPFYRDGISIAAFGAVRNGVFVS 318
           YKVCW  GC ++DILAAID+A+AD V+VLS+SLGG  + +YRDG++I AF A+  G+ VS
Sbjct: 263 YKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVS 322

Query: 319 CSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVKLGSGQVFQGSSLYSGK--NTNQL 378
           CSAGN+GPS S++SNVAPWI TV A   DR FP    LG+G+ F G SL+ G+      L
Sbjct: 323 CSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLL 382

Query: 379 PLVY-NKTAGRQEANLCTPGSLIPAMVKGKIVVCERGTISRTAKGEQVKLAGGAAMILIN 438
           P +Y    +     NLC  G+LIP  VKGKIV+C+RG  +R  KG+ VK AGG  MIL N
Sbjct: 383 PFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILAN 442

Query: 439 TELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQPKGSITFGGTRYGSR-APRVAA 498
           T   GEEL AD H+LPAT++G  AG  I+ ++ +  + P  SI+  GT  G + +P VAA
Sbjct: 443 TAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAA 502

Query: 499 FSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSELESDKRRVLFNIISGTSMSCPHV 558
           FSSRGPN I   I+KPD+ APGVNILAAW     P+ L SD RRV FNIISGTSMSCPHV
Sbjct: 503 FSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHV 562

Query: 559 SGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLISDVGSTSGRPANAFAFGSGHVDP 618
           SGLAALLK+ H +WSPAAI+SALMTTAY T    + + D+   +G+P+  F  G+GHV P
Sbjct: 563 SGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVSP 622

Query: 619 EKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRENFSC-PSRGAFLKPGDLNYPSFS 678
             A++PGL+YD+   DYL +LC+LNYTS QI  +SR N++C PS+       DLNYPSF+
Sbjct: 623 TTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS--YSVADLNYPSFA 682

Query: 679 VIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINS-PKGVAIIVKPQKLSFKRLGEKLSY 738
           V +D   A   +    RTVT+VG   G Y+VK+ S   GV I V+P  L+FK   EK SY
Sbjct: 683 VNVDGVGAYKYT----RTVTSVGG-AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSY 742

Query: 739 KVSFISSGKGKSFGDSSFGSLVWLSGKYSVRSPVAVTW 765
            V+F +    K  G +SFGS+ W  GK+ V SPVA++W
Sbjct: 743 TVTF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MS012307 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 659.1 bits (1699), Expect = 4.4e-189
Identity = 374/785 (47.64%), Postives = 497/785 (63.31%), Query Frame = 0

Query: 1   MGLREVWVVLSIMVA--TSSAAAAVDQRTFIIHMDTMKMPA--TDPEQWYTSMIHSVNEL 60
           + L  ++ V  +++   + S++++    ++I+H+     P+  +    W+ S++ S   L
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS---L 63

Query: 61  PSLDTDEGEASNTAEILYVYKTALSG---NILNIDLDAISKIRGFVAAIPDELLQLHTTH 120
           PS       +   A +LY Y  A+ G    +  I   A+ +    ++ IPD+  ++HTTH
Sbjct: 64  PS-------SPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTH 123

Query: 121 SSNFLGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRRWKGVCEQGPK 180
           +  FLG   + GLW++S    D+IVGVLDTGIWPEH SF D G+  +P  WKG CE GP 
Sbjct: 124 TPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPD 183

Query: 181 FSPSNCNRKLIGAAAFLKGYESIVGRLNQTAT--YRSPRDSDGHGTHTASTAAGNLVYKA 240
           F  S+CNRKLIGA AF +GY +      + A    RSPRD++GHGTHTASTAAG++V  A
Sbjct: 184 FPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANA 243

Query: 241 GFYDQAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSLSLG--GRA 300
             Y  A G A GM   +RIAAYK+CW  GC ++DILAA+D+AVADGV V+SLS+G  G A
Sbjct: 244 SLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSA 303

Query: 301 APFYRDGISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRTFPTTVK 360
             ++ D I+I AFGA R+G+ VSCSAGNSGP+P T +N+APWI+TV AS  DR F     
Sbjct: 304 PEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAI 363

Query: 361 LGSGQVFQGSSLYSGKN--TNQLPLVYNKTAGRQEANLCTPGSLIPAMVKGKIVVCERGT 420
            G G+VF G+SLY+G++   +QL LVY+   G   + LC PG L  ++V+GKIV+C+RG 
Sbjct: 364 TGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGG 423

Query: 421 ISRTAKGEQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIASSKDQ 480
            +R  KG  VKLAGGA MIL NT   GEEL AD H++PAT +GA AG  I+ +I +S D 
Sbjct: 424 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DS 483

Query: 481 PKGSITFGGTRYGSR--APRVAAFSSRGPNPIQRTIIKPDVTAPGVNILAAWPAIISPSE 540
           P   I+F GT  G    +PRVAAFSSRGPN +   I+KPDV APGVNILA W  ++ P++
Sbjct: 484 PTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTD 543

Query: 541 LESDKRRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMRLI 600
           L+ D RRV FNIISGTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N    I
Sbjct: 544 LDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPI 603

Query: 601 SDVGSTSGRPANAFAFGSGHVDPEKASDPGLVYDIMPRDYLNYLCSLNYTSSQIALLSRE 660
            D+   +G+ +N+F  G+GHVDP KA +PGLVYDI  ++Y+ +LC++ Y    I +  ++
Sbjct: 604 EDL--ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQD 663

Query: 661 N--FSCPSRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGS-PRGDYTVKINS 720
              +           GDLNYPSFSV+     +T   V  KR V NVGS     Y V + S
Sbjct: 664 PTLYDACETSKLRTAGDLNYPSFSVVF---ASTGEVVKYKRVVKNVGSNVDAVYEVGVKS 723

Query: 721 PKGVAIIVKPQKLSFKRLGEKLSYKVSFISSGKGKSFGD---SSFGSLVWLSGKYSVRSP 765
           P  V I V P KL+F +    L Y+V+F S   G   G      FGS+ W  G++ V+SP
Sbjct: 724 PANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSP 769