MS012226 (gene) Bitter gourd (TR) v1

Overview


Name	MS012226
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	SASA domain-containing protein
Location	scaffold797: 604394 .. 606519 (+)
RNA-Seq Expression	MS012226
Synteny	MS012226

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTATCATGGCGACGACGAGCGATCCAGATCCAGATCCCCAAAACAAGCAGATCTTCATCTTATCAGGGCAGAGCAACATGGCCGGGCGCGGCGGCGTGTCGAAGAAGCCCAAGCGCTGGGACGGCGTCGTTCCGCCGGAGTCGCAGCCGCACCCCTCGATACTCCGGCTGAGCGCGAAGCTGGAGTGGGAGGCGGCGCGTGAGCCCCTCCACGCGGACATCGACACGAAGAAGACGTGCGGGGTGGGGCCGGGGATGTCGTTCGCAAACGGCGTGAGGGAGCGCGTGGGAGTGGTGGCGCTGGTGCCGTGCGCGGTGGGGGGGACGGCCATCAAGGAGTGGGCGCGTGGAGAGAAGCTGTACGAGGACATGGTGAAGAGGGCGAGGGAGAGCGTGAGGGGCGGCGGAGAGATCAAGGCGCTTCTGTGGTTCCAAGGGGAGAGCGACACCAGTACGGAAGCTGATGCGGTCGCGTACCAGGGGAATATGGAGGAGTTCGTTGCTAATGTGCGCCGCGACCTCGCCTTGCCTTCCCTCCCCATAATTCAGGTACATCACATTTTTATTATCCCATTTATTTTTATTTTCATTATTTTTGGATGGATCTGGAAGATAATTTTCGTTTCTAAAATTTTGACACATTTTCGGAATATTAAGATAAGGACCGGATTCTTTTTCTATTTATCTTCTTTCTTGCTTTTAGTTTTGAATTCTTTTGGTAATTTTTTTATATGAAAATTGATTCCTCAAAATCTTTATGTTGTATTTAGTTTTTCTTTTTAATATTTTTTTATGCATTTTACGGTCAAAAAAGATAAAAAAAATGATGTTTTTTTTCTATGTTTTGAGAGCAAGAATAAGAAATCAGAAAATTGATTTTATTGTTTCTTAAATTTCTAGAACGATTTTTAAAAATAAGAAATGAGAAACAAATATCATTTCTCAAAAATTGAATTTCAAAGTTAGTTTGAATTATACTTCTAACGTTTAAAAAATCACACTGCTACTTGCCACGTTTACTGATAATTTTTACACTTAATCACCACGTCTTATTTTAAATATAAAGTTGTTTTTTTCTTTTTGATATAAAATTATTTAGGACATATTTAAAAAAAATGTGAAGAATAGAAGTGGACTTTTTTTATATGATAGAGTTATTATTGAAATTAACCAAAATAAAAAAGTTCTCAAATACTTGTTATTTTAGCTAAAATAAAATCAAGGAAATATCTTTCTATAATAAAAAAGAAGGAAAAGTAGTTACAAAATTTTATTATTCAGTGACATTAAAAAATATTTTGGACTGATTTGATTAAGAGGAAATCAGATCTCATTGCTAGGCAGCTATAAAATTTTACCGAGTAACAACTTAGTCCTTATATTTTAAGAAGTAAAATTTATAACAACTTAGTTTCTGACATAAAAAATCTCAGGTTGCAGTAACAATTTAATATTTTTAGTTTATAAACACATTTAGTTCTTCACTCAATTTATTGATCAACATATATAAGAAACATTATTGCATCTTTACGACATTTTTTATTACATGGACTAAATTATTATAAATTTGAAAGGACAGAGACTAAATTATAACAATTAAAAAGATAAATAGACGCAATTAAATGTCTTTTTCTTTTAACAATTTAGTCTCTAATTTTAGTACGTAACAATTTAGTATTTTCATTTTAAAATTTGTAACAACTATGAAAATTAAAATCTCATCGAAATTAAATGTCGATCTTCATTACATGACGACTTAGTCTTAAACAGATTTAGTCACTCATTAATTTATTGATGTACCTGTGTAAGAAATTCTATTAAATCCTAATAACATATTTTTCACAATAGAGACTAGACTGTTACAAATTTGAAAATACAGAGACTAAATCGTTATTTATTGAGAAAGTTAGTCGTGGTTGAATGTGAAATGGAAATAGGTGGCACTTGCATCTGGATTTGAGTACATTGAAAAAGTGAGAGAAGCACAATTGGGAATGAAAATAGAGAATGTGGTGTGTGTGGATGCAAAAGGGCTGCAACTCCAAGAAGACAACCTCCACCTCTCAACTCAGTCTCAGGTTGTTTTGGGTCAAATGCTGGCTCAGGCTTACCTCTCCCCAACCCAT

mRNA sequence

GGTATCATGGCGACGACGAGCGATCCAGATCCAGATCCCCAAAACAAGCAGATCTTCATCTTATCAGGGCAGAGCAACATGGCCGGGCGCGGCGGCGTGTCGAAGAAGCCCAAGCGCTGGGACGGCGTCGTTCCGCCGGAGTCGCAGCCGCACCCCTCGATACTCCGGCTGAGCGCGAAGCTGGAGTGGGAGGCGGCGCGTGAGCCCCTCCACGCGGACATCGACACGAAGAAGACGTGCGGGGTGGGGCCGGGGATGTCGTTCGCAAACGGCGTGAGGGAGCGCGTGGGAGTGGTGGCGCTGGTGCCGTGCGCGGTGGGGGGGACGGCCATCAAGGAGTGGGCGCGTGGAGAGAAGCTGTACGAGGACATGGTGAAGAGGGCGAGGGAGAGCGTGAGGGGCGGCGGAGAGATCAAGGCGCTTCTGTGGTTCCAAGGGGAGAGCGACACCAGTACGGAAGCTGATGCGGTCGCGTACCAGGGGAATATGGAGGAGTTCGTTGCTAATGTGCGCCGCGACCTCGCCTTGCCTTCCCTCCCCATAATTCAGGTGGCACTTGCATCTGGATTTGAGTACATTGAAAAAGTGAGAGAAGCACAATTGGGAATGAAAATAGAGAATGTGGTGTGTGTGGATGCAAAAGGGCTGCAACTCCAAGAAGACAACCTCCACCTCTCAACTCAGTCTCAGGTTGTTTTGGGTCAAATGCTGGCTCAGGCTTACCTCTCCCCAACCCAT

Coding sequence (CDS)

GGTATCATGGCGACGACGAGCGATCCAGATCCAGATCCCCAAAACAAGCAGATCTTCATCTTATCAGGGCAGAGCAACATGGCCGGGCGCGGCGGCGTGTCGAAGAAGCCCAAGCGCTGGGACGGCGTCGTTCCGCCGGAGTCGCAGCCGCACCCCTCGATACTCCGGCTGAGCGCGAAGCTGGAGTGGGAGGCGGCGCGTGAGCCCCTCCACGCGGACATCGACACGAAGAAGACGTGCGGGGTGGGGCCGGGGATGTCGTTCGCAAACGGCGTGAGGGAGCGCGTGGGAGTGGTGGCGCTGGTGCCGTGCGCGGTGGGGGGGACGGCCATCAAGGAGTGGGCGCGTGGAGAGAAGCTGTACGAGGACATGGTGAAGAGGGCGAGGGAGAGCGTGAGGGGCGGCGGAGAGATCAAGGCGCTTCTGTGGTTCCAAGGGGAGAGCGACACCAGTACGGAAGCTGATGCGGTCGCGTACCAGGGGAATATGGAGGAGTTCGTTGCTAATGTGCGCCGCGACCTCGCCTTGCCTTCCCTCCCCATAATTCAGGTGGCACTTGCATCTGGATTTGAGTACATTGAAAAAGTGAGAGAAGCACAATTGGGAATGAAAATAGAGAATGTGGTGTGTGTGGATGCAAAAGGGCTGCAACTCCAAGAAGACAACCTCCACCTCTCAACTCAGTCTCAGGTTGTTTTGGGTCAAATGCTGGCTCAGGCTTACCTCTCCCCAACCCAT

Protein sequence

GIMATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYLSPTH

Homology

[Close]

BLAST of MS012226 vs. NCBI nr
Match: XP_022148694.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 488.8 bits (1257), Expect = 2.7e-134
Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 0


Query: 3   MATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLE 62
           MATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLE
Sbjct: 1   MATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLE 60

Query: 63  WEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYE 122
           WEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYE
Sbjct: 61  WEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYE 120

Query: 123 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 182
           DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII
Sbjct: 121 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 180

Query: 183 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 242
           QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL
Sbjct: 181 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 240

Query: 243 SPTH 247
           SPTH
Sbjct: 241 SPTH 244

[Close]

BLAST of MS012226 vs. NCBI nr
Match: XP_023553837.1 (probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 414.8 bits (1065), Expect = 5.0e-112
Identity = 208/249 (83.53%), Postives = 222/249 (89.16%), Query Frame = 0


Query: 3   MATTSDPDP--------DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSI 62
           MA T+D DP         P NKQIFILSGQSNMAGRGGV KK  RWDGVVPPE+QPHPSI
Sbjct: 1   MAATTDLDPIQTNATPNPPPNKQIFILSGQSNMAGRGGVLKKLHRWDGVVPPEAQPHPSI 60

Query: 63  LRLSAKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEW 122
            RLSAKL WE A EPLHADIDTKKTCGVGPGM+FANGVRERVG VALVPCAVGGTAIKEW
Sbjct: 61  FRLSAKLHWEVAHEPLHADIDTKKTCGVGPGMAFANGVRERVGTVALVPCAVGGTAIKEW 120

Query: 123 ARGEKLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDL 182
           ARGEKLYEDMVKRAR SV+ GGEI+A+LWFQGESDTSTE DA AYQGNME FVANVRRDL
Sbjct: 121 ARGEKLYEDMVKRARHSVKDGGEIRAILWFQGESDTSTEHDADAYQGNMEAFVANVRRDL 180

Query: 183 ALPSLPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLG 242
           ALPSLPIIQVALASG +YIEKVREAQLGM++ENVVCVDAKGL+LQEDNLHL+TQ+QV+LG
Sbjct: 181 ALPSLPIIQVALASGVKYIEKVREAQLGMRVENVVCVDAKGLELQEDNLHLTTQAQVILG 240

Query: 243 QMLAQAYLS 244
           QMLA AYL+
Sbjct: 241 QMLADAYLT 249

[Close]

BLAST of MS012226 vs. NCBI nr
Match: XP_008455001.1 (PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo])

HSP 1 Score: 411.0 bits (1055), Expect = 7.3e-111
Identity = 204/245 (83.27%), Postives = 222/245 (90.61%), Query Frame = 0


Query: 3   MATTSDPDP----DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLS 62
           MATT+D DP     P NKQIFILSGQSNM+GRGGV KK ++WDGVVPPE+QPHPSI RLS
Sbjct: 1   MATTTDTDPIHADPPPNKQIFILSGQSNMSGRGGVLKKLRQWDGVVPPEAQPHPSIFRLS 60

Query: 63  AKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGE 122
           AK  WEAAREPLHADIDTKKTCGVGPGM FANGVRERVG VALVPCAVGGTAI+EWARGE
Sbjct: 61  AKKHWEAAREPLHADIDTKKTCGVGPGMVFANGVRERVGTVALVPCAVGGTAIREWARGE 120

Query: 123 KLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPS 182
           KLYE+MVKRARESV+GGGEIKA+LWFQGESDT+TE DA AY+GNME FVANVRRDLALPS
Sbjct: 121 KLYEEMVKRARESVKGGGEIKAILWFQGESDTTTEHDADAYRGNMEAFVANVRRDLALPS 180

Query: 183 LPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLA 242
           LPIIQVALASG +YIEKVREAQLGMK+EN+VCVDA GL+LQEDNLHL+T SQV+LGQML 
Sbjct: 181 LPIIQVALASGLKYIEKVREAQLGMKMENLVCVDAMGLELQEDNLHLTTPSQVILGQMLV 240

Query: 243 QAYLS 244
            AY +
Sbjct: 241 DAYFT 245

[Close]

BLAST of MS012226 vs. NCBI nr
Match: KAG7011640.1 (putative carbohydrate esterase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 410.6 bits (1054), Expect = 9.5e-111
Identity = 205/249 (82.33%), Postives = 221/249 (88.76%), Query Frame = 0


Query: 3   MATTSDPDP--------DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSI 62
           MA T+D DP         P NKQIFILSGQSNMAGRGGV KK  RWDGVVPPE+QPHPSI
Sbjct: 1   MAATTDLDPIQTNATTNPPPNKQIFILSGQSNMAGRGGVLKKLHRWDGVVPPEAQPHPSI 60

Query: 63  LRLSAKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEW 122
            RLSAKL WE A EPLHADIDTKKTCGVGPGM+FANGVRERVG VALVPCAVGGTAIKEW
Sbjct: 61  FRLSAKLHWEVAHEPLHADIDTKKTCGVGPGMAFANGVRERVGTVALVPCAVGGTAIKEW 120

Query: 123 ARGEKLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDL 182
           ARGEKLYEDMVKRAR SV+ GGEI+A+LWFQGESDTSTE DA AYQGNME FVANVRRDL
Sbjct: 121 ARGEKLYEDMVKRARHSVKDGGEIRAILWFQGESDTSTEHDADAYQGNMEAFVANVRRDL 180

Query: 183 ALPSLPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLG 242
            LPSLPIIQVALASG +YIE+VREAQLGM++ENVVCVDAKGL+L+EDNLHL+TQ+QV+LG
Sbjct: 181 TLPSLPIIQVALASGVKYIERVREAQLGMRVENVVCVDAKGLELKEDNLHLTTQAQVILG 240

Query: 243 QMLAQAYLS 244
           QMLA AYL+
Sbjct: 241 QMLADAYLT 249

[Close]

BLAST of MS012226 vs. NCBI nr
Match: XP_022952956.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 409.8 bits (1052), Expect = 1.6e-110
Identity = 205/249 (82.33%), Postives = 221/249 (88.76%), Query Frame = 0


Query: 3   MATTSDPDP--------DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSI 62
           MA T+D DP         P NKQIFILSGQSNMAGRGGV KK  RWDGVVPPE+QPHPSI
Sbjct: 1   MAATTDLDPIQTNAPPNPPPNKQIFILSGQSNMAGRGGVLKKLHRWDGVVPPEAQPHPSI 60

Query: 63  LRLSAKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEW 122
            RL AKL WE A EPLHADIDTKKTCGVGPGM+FANGVRERVG VALVPCAVGGTAIKEW
Sbjct: 61  FRLIAKLHWEVAHEPLHADIDTKKTCGVGPGMAFANGVRERVGTVALVPCAVGGTAIKEW 120

Query: 123 ARGEKLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDL 182
           ARGEKLYEDMVKRAR SV+ GGEI+A+LWFQGESDTSTE DA AYQGNME FVANVRRDL
Sbjct: 121 ARGEKLYEDMVKRARHSVKDGGEIRAILWFQGESDTSTEHDADAYQGNMEAFVANVRRDL 180

Query: 183 ALPSLPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLG 242
           ALPSLPIIQVALASG +YIE+VREAQLGM++ENVVCVDAKGL+L+EDNLHL+TQ+QV+LG
Sbjct: 181 ALPSLPIIQVALASGVKYIERVREAQLGMRVENVVCVDAKGLELKEDNLHLTTQAQVILG 240

Query: 243 QMLAQAYLS 244
           QMLA AYL+
Sbjct: 241 QMLADAYLT 249

[Close]

BLAST of MS012226 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-80
Identity = 158/241 (65.56%), Postives = 180/241 (74.69%), Query Frame = 0


Query: 11  PDPQNKQIFILSGQSNMAGRGGVSKKPKR----WDGVVPPESQPHPSILRLSAKLEWEAA 70
           P P N QIFILSGQSNMAGRGGV K        WD ++PPE  P+ SILRLSA L WE A
Sbjct: 18  PIPPN-QIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEA 77

Query: 71  REPLHADIDTKKTCGVGPGMSFANGVRERV----GVVALVPCAVGGTAIKEWARGEKLYE 130
            EPLH DIDT K CGVGPGM+FAN V+ R+     V+ LVPCA GGTAIKEW RG  LYE
Sbjct: 78  HEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYE 137

Query: 131 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 190
            MVKR  ES + GGEIKA+LW+QGESD     DA +Y  NM+  + N+R DL LPSLPII
Sbjct: 138 RMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPII 197

Query: 191 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 244
           QVA+ASG  YI+KVREAQLG+K+ NVVCVDAKGL L+ DNLHL+T++QV LG  LAQAYL
Sbjct: 198 QVAIASGGGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQAYL 257

[Close]

BLAST of MS012226 vs. ExPASy TrEMBL
Match: A0A6J1D4Q9 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111017295 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.3e-134
Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 0


Query: 3   MATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLE 62
           MATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLE
Sbjct: 1   MATTSDPDPDPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLSAKLE 60

Query: 63  WEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYE 122
           WEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYE
Sbjct: 61  WEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYE 120

Query: 123 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 182
           DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII
Sbjct: 121 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 180

Query: 183 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 242
           QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL
Sbjct: 181 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 240

Query: 243 SPTH 247
           SPTH
Sbjct: 241 SPTH 244

[Close]

BLAST of MS012226 vs. ExPASy TrEMBL
Match: A0A1S3BZF3 (probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103495282 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.5e-111
Identity = 204/245 (83.27%), Postives = 222/245 (90.61%), Query Frame = 0


Query: 3   MATTSDPDP----DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLS 62
           MATT+D DP     P NKQIFILSGQSNM+GRGGV KK ++WDGVVPPE+QPHPSI RLS
Sbjct: 1   MATTTDTDPIHADPPPNKQIFILSGQSNMSGRGGVLKKLRQWDGVVPPEAQPHPSIFRLS 60

Query: 63  AKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGE 122
           AK  WEAAREPLHADIDTKKTCGVGPGM FANGVRERVG VALVPCAVGGTAI+EWARGE
Sbjct: 61  AKKHWEAAREPLHADIDTKKTCGVGPGMVFANGVRERVGTVALVPCAVGGTAIREWARGE 120

Query: 123 KLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPS 182
           KLYE+MVKRARESV+GGGEIKA+LWFQGESDT+TE DA AY+GNME FVANVRRDLALPS
Sbjct: 121 KLYEEMVKRARESVKGGGEIKAILWFQGESDTTTEHDADAYRGNMEAFVANVRRDLALPS 180

Query: 183 LPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLA 242
           LPIIQVALASG +YIEKVREAQLGMK+EN+VCVDA GL+LQEDNLHL+T SQV+LGQML 
Sbjct: 181 LPIIQVALASGLKYIEKVREAQLGMKMENLVCVDAMGLELQEDNLHLTTPSQVILGQMLV 240

Query: 243 QAYLS 244
            AY +
Sbjct: 241 DAYFT 245

[Close]

BLAST of MS012226 vs. ExPASy TrEMBL
Match: A0A6J1GLP0 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111455478 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.8e-111
Identity = 205/249 (82.33%), Postives = 221/249 (88.76%), Query Frame = 0


Query: 3   MATTSDPDP--------DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSI 62
           MA T+D DP         P NKQIFILSGQSNMAGRGGV KK  RWDGVVPPE+QPHPSI
Sbjct: 1   MAATTDLDPIQTNAPPNPPPNKQIFILSGQSNMAGRGGVLKKLHRWDGVVPPEAQPHPSI 60

Query: 63  LRLSAKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEW 122
            RL AKL WE A EPLHADIDTKKTCGVGPGM+FANGVRERVG VALVPCAVGGTAIKEW
Sbjct: 61  FRLIAKLHWEVAHEPLHADIDTKKTCGVGPGMAFANGVRERVGTVALVPCAVGGTAIKEW 120

Query: 123 ARGEKLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDL 182
           ARGEKLYEDMVKRAR SV+ GGEI+A+LWFQGESDTSTE DA AYQGNME FVANVRRDL
Sbjct: 121 ARGEKLYEDMVKRARHSVKDGGEIRAILWFQGESDTSTEHDADAYQGNMEAFVANVRRDL 180

Query: 183 ALPSLPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLG 242
           ALPSLPIIQVALASG +YIE+VREAQLGM++ENVVCVDAKGL+L+EDNLHL+TQ+QV+LG
Sbjct: 181 ALPSLPIIQVALASGVKYIERVREAQLGMRVENVVCVDAKGLELKEDNLHLTTQAQVILG 240

Query: 243 QMLAQAYLS 244
           QMLA AYL+
Sbjct: 241 QMLADAYLT 249

[Close]

BLAST of MS012226 vs. ExPASy TrEMBL
Match: A0A6J1I4P5 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111470965 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.3e-110
Identity = 204/249 (81.93%), Postives = 221/249 (88.76%), Query Frame = 0


Query: 3   MATTSDPDP--------DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSI 62
           MA T+D  P         P NKQIFILSGQSNMAGRGGV KK  RWDGVVPPE+QPHPSI
Sbjct: 1   MAATTDLGPIQTNATSNPPPNKQIFILSGQSNMAGRGGVLKKLHRWDGVVPPEAQPHPSI 60

Query: 63  LRLSAKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEW 122
            RLSAKL WE A EPLHADID+KKTCGVGPGM+FANGVRERVG VALVPCAVGGTAI+EW
Sbjct: 61  FRLSAKLHWEVAHEPLHADIDSKKTCGVGPGMAFANGVRERVGTVALVPCAVGGTAIREW 120

Query: 123 ARGEKLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDL 182
           ARGEKLYEDMVKRAR SV+ GGEI+A+LWFQGESDTSTE DA AYQGNME FVANVRRDL
Sbjct: 121 ARGEKLYEDMVKRARHSVKDGGEIRAILWFQGESDTSTEHDADAYQGNMEAFVANVRRDL 180

Query: 183 ALPSLPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLG 242
           ALPSLPIIQVALASG +YIEKVREAQLGM++ENVVCVDAKGL+L+EDNLHL+TQ+QV+LG
Sbjct: 181 ALPSLPIIQVALASGIKYIEKVREAQLGMRVENVVCVDAKGLELKEDNLHLTTQAQVILG 240

Query: 243 QMLAQAYLS 244
           QMLA AYL+
Sbjct: 241 QMLADAYLT 249

[Close]

BLAST of MS012226 vs. ExPASy TrEMBL
Match: A0A0A0K303 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071670 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.9e-109
Identity = 202/245 (82.45%), Postives = 218/245 (88.98%), Query Frame = 0


Query: 3   MATTSDPDP----DPQNKQIFILSGQSNMAGRGGVSKKPKRWDGVVPPESQPHPSILRLS 62
           MATT+D DP     P NKQIFILSGQSNMAGRGGV KK +RWDGVVPPE+ PHPSI RLS
Sbjct: 1   MATTTDTDPIHTDPPPNKQIFILSGQSNMAGRGGVLKKLRRWDGVVPPEAHPHPSIFRLS 60

Query: 63  AKLEWEAAREPLHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGE 122
           AK  WEAA EPLHADIDTKKTCGVGPGM FANGVRERVG VALVPCAVGGTAI+EWARGE
Sbjct: 61  AKKHWEAACEPLHADIDTKKTCGVGPGMVFANGVRERVGTVALVPCAVGGTAIREWARGE 120

Query: 123 KLYEDMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPS 182
           KLYE+MVKRAR+SV+GGGEIKA+LWFQGESDTSTE DA AYQGNME  VANVRRDLALPS
Sbjct: 121 KLYEEMVKRARDSVKGGGEIKAILWFQGESDTSTEHDADAYQGNMEALVANVRRDLALPS 180

Query: 183 LPIIQVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLA 242
           LPIIQVALASG +Y +KVREAQLGMK+EN+VCVDA GL+LQEDNLHL+T SQV+LGQML 
Sbjct: 181 LPIIQVALASGLKYTDKVREAQLGMKMENLVCVDAMGLELQEDNLHLTTHSQVILGQMLV 240

Query: 243 QAYLS 244
            AY +
Sbjct: 241 DAYFT 245

[Close]

BLAST of MS012226 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 301.2 bits (770), Expect = 7.6e-82
Identity = 158/241 (65.56%), Postives = 180/241 (74.69%), Query Frame = 0


Query: 11  PDPQNKQIFILSGQSNMAGRGGVSKKPKR----WDGVVPPESQPHPSILRLSAKLEWEAA 70
           P P N QIFILSGQSNMAGRGGV K        WD ++PPE  P+ SILRLSA L WE A
Sbjct: 18  PIPPN-QIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEA 77

Query: 71  REPLHADIDTKKTCGVGPGMSFANGVRERV----GVVALVPCAVGGTAIKEWARGEKLYE 130
            EPLH DIDT K CGVGPGM+FAN V+ R+     V+ LVPCA GGTAIKEW RG  LYE
Sbjct: 78  HEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYE 137

Query: 131 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 190
            MVKR  ES + GGEIKA+LW+QGESD     DA +Y  NM+  + N+R DL LPSLPII
Sbjct: 138 RMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPII 197

Query: 191 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 244
           QVA+ASG  YI+KVREAQLG+K+ NVVCVDAKGL L+ DNLHL+T++QV LG  LAQAYL
Sbjct: 198 QVAIASGGGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQAYL 257

[Close]

BLAST of MS012226 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 301.2 bits (770), Expect = 7.6e-82
Identity = 158/241 (65.56%), Postives = 180/241 (74.69%), Query Frame = 0


Query: 11  PDPQNKQIFILSGQSNMAGRGGVSKKPKR----WDGVVPPESQPHPSILRLSAKLEWEAA 70
           P P N QIFILSGQSNMAGRGGV K        WD ++PPE  P+ SILRLSA L WE A
Sbjct: 18  PIPPN-QIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEA 77

Query: 71  REPLHADIDTKKTCGVGPGMSFANGVRERV----GVVALVPCAVGGTAIKEWARGEKLYE 130
            EPLH DIDT K CGVGPGM+FAN V+ R+     V+ LVPCA GGTAIKEW RG  LYE
Sbjct: 78  HEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGSHLYE 137

Query: 131 DMVKRARESVRGGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPII 190
            MVKR  ES + GGEIKA+LW+QGESD     DA +Y  NM+  + N+R DL LPSLPII
Sbjct: 138 RMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPII 197

Query: 191 QVALASGFEYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYL 244
           QVA+ASG  YI+KVREAQLG+K+ NVVCVDAKGL L+ DNLHL+T++QV LG  LAQAYL
Sbjct: 198 QVAIASGGGYIDKVREAQLGLKLSNVVCVDAKGLPLKSDNLHLTTEAQVQLGLSLAQAYL 257

[Close]

BLAST of MS012226 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 261.2 bits (666), Expect = 8.7e-70
Identity = 132/237 (55.70%), Postives = 174/237 (73.42%), Query Frame = 0


Query: 14  QNKQIFILSGQSNMAGRGGV----SKKPKRWDGVVPPESQPHPSILRLSAKLEWEAAREP 73
           +N  IFIL+GQSNMAGRGGV    +     WDGV+PPE + +PSILRL++KLEW+ A+EP
Sbjct: 27  RNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEP 86

Query: 74  LHADIDTKKTCGVGPGMSFANGVRERVGVVALVPCAVGGTAIKEWARGEKLYEDMVKRAR 133
           LH DID  KT GVGPGM FAN V  R G V LVPC++GGT + +W +GE LYE+ VKRA+
Sbjct: 87  LHVDIDINKTNGVGPGMPFANRVVNRFGQVGLVPCSIGGTKLSQWQKGEFLYEETVKRAK 146

Query: 134 ESVR--GGGEIKALLWFQGESDTSTEADAVAYQGNMEEFVANVRRDLALPSLPIIQVALA 193
            ++   GGG  +A+LW+QGESDT    DA  Y+  + +F +++R DL  P+LPIIQVALA
Sbjct: 147 AAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPIIQVALA 206

Query: 194 SGF-EYIEKVREAQLGMKIENVVCVDAKGLQLQEDNLHLSTQSQVVLGQMLAQAYLS 244
           +G   Y++ VR+AQL   +ENV CVDA+GL L+ D LHL+T SQV LG M+A+++L+
Sbjct: 207 TGAGPYLDAVRKAQLKTDLENVYCVDARGLPLEPDGLHLTTSSQVQLGHMIAESFLA 263

The following BLAST results are available for this feature:

BLAST of MS012226 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148694.1	2.7e-134	100.00	probable carbohydrate esterase At4g34215 [Momordica charantia]	[more]
XP_023553837.1	5.0e-112	83.53	probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo]	[more]
XP_008455001.1	7.3e-111	83.27	PREDICTED: probable carbohydrate esterase At4g34215 [Cucumis melo]	[more]
KAG7011640.1	9.5e-111	82.33	putative carbohydrate esterase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022952956.1	1.6e-110	82.33	probable carbohydrate esterase At4g34215 [Cucurbita moschata]	[more]

BLAST of MS012226 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9J9	1.1e-80	65.56	Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g...	[more]

BLAST of MS012226 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4Q9	1.3e-134	100.00	probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A1S3BZF3	3.5e-111	83.27	probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103495282...	[more]
A0A6J1GLP0	7.8e-111	82.33	probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1I4P5	2.3e-110	81.93	probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A0A0K303	1.9e-109	82.45	SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071670 PE=4 S...	[more]

BLAST of MS012226 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34215.1	7.6e-82	65.56	Domain of unknown function (DUF303)	[more]
AT4G34215.2	7.6e-82	65.56	Domain of unknown function (DUF303)	[more]
AT3G53010.1	8.7e-70	55.70	Domain of unknown function (DUF303)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005181	Sialate O-acetylesterase domain	PFAM	PF03629	SASA	coord: 16..242 e-value: 8.5E-90 score: 300.3
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 15..243 e-value: 3.7E-67 score: 228.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..51
None	No IPR available	PANTHER	PTHR31988:SF19	BNACNNG62850D PROTEIN	coord: 13..243
None	No IPR available	PANTHER	PTHR31988	ESTERASE, PUTATIVE (DUF303)-RELATED	coord: 13..243
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 17..244

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012226.1	MS012226.1	mRNA