MS012222 (gene) Bitter gourd (TR) v1

Overview


Name	MS012222
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNA pseudouridine synthase 4, mitochondrial
Location	scaffold797: 559105 .. 562410 (+)
RNA-Seq Expression	MS012222
Synteny	MS012222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTACTTCCGGCGCTCCTCCGTCGTTCCCTCATCCTTAGTGGCACCCCATCTTCCACCGCTCGCGGCGGCCACAGCCTCCATTTCTCGGTTTTTCACGAGCTGAAATCTACATTCGCCTTGAGCAACACCTCCGAAACCATTTCCGAAGAGAAGAATGAGAAGAAAGGATTTAATAGATGGTTCACGTTGCCTCCCTACACTGCGACTGTCGACGGCTCGGTTTTAGGTAATCGAATTCTGAACCAAGGATCTCAAGCAGAAGATGAAACTTCCACTACTGCAATCACCGCTCTGAAATGGGTGCTTCGCTGCTGCCCGGAGCTGCCAAGGAGCCTCGTACAGAAGCTATTTCGCCTTAGAAAGGTTCCGTCCTAAATCTCACTCAATACTCAGTTAAGTTAAAGCAATTGAATGATCCGTGAAGCTAGCTAAGATAATGCCTTCGGATTCTATCAATTGTCCGTCTTATCATGCAATTCAATGTTTATGTGGTAATGCATTGGCAAATTTCGAGTTGATTGTTAAACATCTTCCTCAGTTTTTATGAATCTTTTCCTTTTGGCATCTTTCAGATTCGAAGAGGTTCTTCTATGATTGAGAGTTCGGAATGTAATTTGGAAGCTCAAAACCAACTTAAAAGAGTAAGCAGTTATTTTCTTTCTTACATGGATATTAGTTACTATGTTTATGTTCTGATTCAGAGAACTCTGATTATGCACGCTGCAATTCATTGAAGGTTATCATAATGCTTTCCATACGGAAGAAACTTTCTTAGTTTCTTACTAACATTTTAAGTTATTGCAAGGTGGCAGCCAAGGATATTTTGAACGTAGGTGATCAAATTTTTCTCCCTATCTCGGTTCAAGTATTTCCTAGAGAGAGAGCACGGGATTATTGCAATAAAGAAGAAGAGAAATTTATGCATAGTCTAGTTTTGTATAAGGTTTTCACTTTTCACTGAATTTCCCCCTCTGTTAACAATTCTTAGTTCCTCCTGCTTCATGTGACCCACATTTATTATGTCAGGATCCAGCAATAATTGTTATCAATAAACCTCCTGGCTTGCCTGTTCAGGTATGGTGTCCAACTTGTACCATTGATCCTTATGAATAATCATGGTCGTAGAGTTAACAAAATTATAGCAAATAAAAATGTTTGTGCAGGGTGGTATTGGCATCACAAGGAGTTTAGACGAACTGGCTGCAAGTTGTCTAAGTTATGACTACTCAGAACCACCTCGGCTTGTAAGTTTTTCTTCAAATCATTGATTTTGTTGTATTCCTAATTCTTGAGCGTTTCCTTCTACATGTTGGAATCAATCAGAAAATAGTGTACTTGTGCTTGGAGTTAATTAATGGTGTTTGAACTTGACCTTTAAATGATGAACTCTGACATTGGTCATTTTGTTCAACTTCATTAAACAACATGCCATAAAATGATTTATATAAAATCATTTGTGGTGAAGTTTGTATCTTTGAACATAAAGTTCGTTTCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAACATTTGTGGTGTTTCTACTTTCTAGTTTGATCTTTCCCCTTTGGTGATGGGCGGTTCATATATTGAAATGTTTTATCTGAACAAAGATGTGGGCTTTGCTTTTGCCAGGTACATAGGCTTGACAGGGACAGTAGTGGCATCTTGGTAATGGGCAGGACTTCCACAAGTACTTCAGTTCTCCATTCTATCTTCCGTGAGAAAACTTTTGGGGCATCAAAACATGTACTTCTCTCCCTACCTAAAACAATATAACAATCTGGAGAAAAAAAAGGCAGAAGTATTGATATTATTAAAATTAACATGGTTATTTGTTTTACTTTTTCTTGCATCAAAGCATGTTGATAGTGAGAAAAAGATTTTGCAGAAAAAGTATTGGGCACTTGTAATTGGTTCTCCGAGATGTCCAAATGGATTGATTACAGCCCCACTAGGAAAGGTTTGTGTAAAATTGCTTTCAAGAAACTATCTTGGAAGTTTTATCTTATATTGATAACCATACATTGCATTGATTTCTTTGTGATTATTAACTGTTTCTTTCCTCATCTATTGTCTAGAAATTTATGTGCGCCCTCTAATTATTTTAGATTGTGCTGGACGATGGAAGATCTGAGAAAATTATGGTCATTGACAATGCCAAATCTATGGCTTTTCAGCAAGCAATTACTACTTATAGAGTGATTAAATCTCATCATGGTTTGCACTTCACCATTTTACACAGCATTTGTTGATTCGCATTTGATTTTTCAAGTGGAACATATTTTTAATCCGAATAGTCAAGTCATAATGGAATCATCGTATGAACATGCATCAGATTGTAGACTTAGTAGGTTTCTTAATTCTCTTTACTACTTGGGTAGTTTGTATTGGGACATGAGATCGTTTCTTCAGTATCCTGTTTATGATAAAGCTTGCAGCTGATCATTTGGCTGGGAAGGGATTTTTTAAAATCGAGGGGGTAGGAAATGTCTGAGAACTTGTACTCGTTATTTTTTCTCTGTAGTTCATAATGAGAAAATTTTTATACCATCACCGTCTAAGTTAGCTAGAAGACAGCTTTATGCTCAAGTAGTCAGTTACAGTTCTACTTACCTGGCTTTTAAAAGAATGCCTCACGAGTAAAAGAAAAGGCACTTTGCACTCTGTTATTTTTCATCACAACTCACGAGAATCAATCGTTCGAATAAATCCTATCTATTGAAACTAATTTCATTCATGTATAACTTGAATGCACCTATCTTAACTATGCTAGAGAAAACATCAGGCTCCCTTCATTTATTACATGGCCATAATGCAGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGGTGCCATCCAGCATCAATGTTTCCAGATACTATGAGCATTTAGTCTCTTTTTCTACATATTTTTGGCTGAATATGCCTTGCATTGTATGTGTTTAATGCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACACCGATAGTTGGCGACTACAAATATGGATGGCAAGCACATAGAAATTGGGAACCATTTCCTTCTAATTTCCACAAGAACCAAGGAAAGAAACAATCCCTTCCATTTGGTCTTGATTTTGAAAATGGAAGTGTTGCAGAAAATACCCCTCGCCTACACCTTCACTGTAAACAATTGGCTTTACCAAATGTTTCTGGCGCACTGCCTAGTGATTCTCTAGATCATGACTTCTCAGAATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCGGAGAAGTTGGGATATCATAAATAAT

mRNA sequence

ATGCTACTTCCGGCGCTCCTCCGTCGTTCCCTCATCCTTAGTGGCACCCCATCTTCCACCGCTCGCGGCGGCCACAGCCTCCATTTCTCGGTTTTTCACGAGCTGAAATCTACATTCGCCTTGAGCAACACCTCCGAAACCATTTCCGAAGAGAAGAATGAGAAGAAAGGATTTAATAGATGGTTCACGTTGCCTCCCTACACTGCGACTGTCGACGGCTCGGTTTTAGGTAATCGAATTCTGAACCAAGGATCTCAAGCAGAAGATGAAACTTCCACTACTGCAATCACCGCTCTGAAATGGGTGCTTCGCTGCTGCCCGGAGCTGCCAAGGAGCCTCGTACAGAAGCTATTTCGCCTTAGAAAGATTCGAAGAGGTTCTTCTATGATTGAGAGTTCGGAATGTAATTTGGAAGCTCAAAACCAACTTAAAAGAGTGGCAGCCAAGGATATTTTGAACGTAGGTGATCAAATTTTTCTCCCTATCTCGGTTCAAGTATTTCCTAGAGAGAGAGCACGGGATTATTGCAATAAAGAAGAAGAGAAATTTATGCATAGTCTAGTTTTGTATAAGGATCCAGCAATAATTGTTATCAATAAACCTCCTGGCTTGCCTGTTCAGGGTGGTATTGGCATCACAAGGAGTTTAGACGAACTGGCTGCAAGTTGTCTAAGTTATGACTACTCAGAACCACCTCGGCTTGTACATAGGCTTGACAGGGACAGTAGTGGCATCTTGGTAATGGGCAGGACTTCCACAAGTACTTCAGTTCTCCATTCTATCTTCCGTGAGAAAACTTTTGGGGCATCAAAACATCATGTTGATAGTGAGAAAAAGATTTTGCAGAAAAAGTATTGGGCACTTGTAATTGGTTCTCCGAGATGTCCAAATGGATTGATTACAGCCCCACTAGGAAAGATTGTGCTGGACGATGGAAGATCTGAGAAAATTATGGTCATTGACAATGCCAAATCTATGGCTTTTCAGCAAGCAATTACTACTTATAGAGTGATTAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACACCGATAGTTGGCGACTACAAATATGGATGGCAAGCACATAGAAATTGGGAACCATTTCCTTCTAATTTCCACAAGAACCAAGGAAAGAAACAATCCCTTCCATTTGGTCTTGATTTTGAAAATGGAAGTGTTGCAGAAAATACCCCTCGCCTACACCTTCACTGTAAACAATTGGCTTTACCAAATGTTTCTGGCGCACTGCCTAGTGATTCTCTAGATCATGACTTCTCAGAATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCGGAGAAGTTGGGATATCATAAATAAT

Coding sequence (CDS)

ATGCTACTTCCGGCGCTCCTCCGTCGTTCCCTCATCCTTAGTGGCACCCCATCTTCCACCGCTCGCGGCGGCCACAGCCTCCATTTCTCGGTTTTTCACGAGCTGAAATCTACATTCGCCTTGAGCAACACCTCCGAAACCATTTCCGAAGAGAAGAATGAGAAGAAAGGATTTAATAGATGGTTCACGTTGCCTCCCTACACTGCGACTGTCGACGGCTCGGTTTTAGGTAATCGAATTCTGAACCAAGGATCTCAAGCAGAAGATGAAACTTCCACTACTGCAATCACCGCTCTGAAATGGGTGCTTCGCTGCTGCCCGGAGCTGCCAAGGAGCCTCGTACAGAAGCTATTTCGCCTTAGAAAGATTCGAAGAGGTTCTTCTATGATTGAGAGTTCGGAATGTAATTTGGAAGCTCAAAACCAACTTAAAAGAGTGGCAGCCAAGGATATTTTGAACGTAGGTGATCAAATTTTTCTCCCTATCTCGGTTCAAGTATTTCCTAGAGAGAGAGCACGGGATTATTGCAATAAAGAAGAAGAGAAATTTATGCATAGTCTAGTTTTGTATAAGGATCCAGCAATAATTGTTATCAATAAACCTCCTGGCTTGCCTGTTCAGGGTGGTATTGGCATCACAAGGAGTTTAGACGAACTGGCTGCAAGTTGTCTAAGTTATGACTACTCAGAACCACCTCGGCTTGTACATAGGCTTGACAGGGACAGTAGTGGCATCTTGGTAATGGGCAGGACTTCCACAAGTACTTCAGTTCTCCATTCTATCTTCCGTGAGAAAACTTTTGGGGCATCAAAACATCATGTTGATAGTGAGAAAAAGATTTTGCAGAAAAAGTATTGGGCACTTGTAATTGGTTCTCCGAGATGTCCAAATGGATTGATTACAGCCCCACTAGGAAAGATTGTGCTGGACGATGGAAGATCTGAGAAAATTATGGTCATTGACAATGCCAAATCTATGGCTTTTCAGCAAGCAATTACTACTTATAGAGTGATTAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCCGAGGTATTAGGAACACCGATAGTTGGCGACTACAAATATGGATGGCAAGCACATAGAAATTGGGAACCATTTCCTTCTAATTTCCACAAGAACCAAGGAAAGAAACAATCCCTTCCATTTGGTCTTGATTTTGAAAATGGAAGTGTTGCAGAAAATACCCCTCGCCTACACCTTCACTGTAAACAATTGGCTTTACCAAATGTTTCTGGCGCACTGCCTAGTGATTCTCTAGATCATGACTTCTCAGAATTGGAATGTCTGGAGTTGGATGCTCCTTTGCCTTCATACATGCGGAGAAGTTGGGATATCATAAATAAT

Protein sequence

MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNRWFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRLRKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEEEKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRCPNGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTPRLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN

Homology

[Close]

BLAST of MS012222 vs. NCBI nr
Match: XP_022148845.1 (RNA pseudouridine synthase 4, mitochondrial [Momordica charantia])

HSP 1 Score: 953.7 bits (2464), Expect = 5.7e-274
Identity = 466/470 (99.15%), Postives = 469/470 (99.79%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRCPNGLI 300
           DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPR PNGLI
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRRPNGLI 300

Query: 301 TAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR 360
           TAPLGKIVLDDGRSEKIMVIDNA+SMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR
Sbjct: 301 TAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR 360

Query: 361 VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTPRL 420
           VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAEN+PRL
Sbjct: 361 VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSPRL 420

Query: 421 HLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           HLHCKQLALPNVSGALP+DSLDHDFSELECLELDAPLPSYMRRSWDIINN
Sbjct: 421 HLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

[Close]

BLAST of MS012222 vs. NCBI nr
Match: XP_038888757.1 (RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 809.7 bits (2090), Expect = 1.3e-230
Identity = 393/471 (83.44%), Postives = 429/471 (91.08%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LPAL+RRS+IL    SS A  G S HFS+FHELKST ALSNT+ET+S  KNE KG NR
Sbjct: 1   MVLPALIRRSVILRIPLSSAAPVGESFHFSIFHELKSTLALSNTTETVSHRKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV+GS+LGNRIL +G QAE E ST AITALKWV+RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVNGSLLGNRILKRGDQAEAEISTAAITALKWVIRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMI +SECNLEA++QLKRVAAKDILN+GDQIFLPISVQVFP E+ARDYCNKEE
Sbjct: 121 RKVRRGSSMIGNSECNLEARDQLKRVAAKDILNIGDQIFLPISVQVFPTEKARDYCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           EKF+HSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 EKFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTS+LHS+FREKTF ASKH  HVDSEKKILQK+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSLLHSVFREKTFEASKHANHVDSEKKILQKRYWALVIGSPRRSKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPLGK V+DDGRSE+IMVIDNA+SM FQQAIT Y+VIKSHHGYTWLELCP+TGRKHQ
Sbjct: 301 LITAPLGKFVMDDGRSEQIMVIDNAESMPFQQAITRYKVIKSHHGYTWLELCPVTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNW+PF SNFHKN+GKKQS PFGLDFE+GS+AEN P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSFPFGLDFESGSIAENNP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIIN 470
           RLHLHCKQL LP+VS AL +DS+D   S LECLELDAPLP YM+RSWDIIN
Sbjct: 421 RLHLHCKQLTLPDVSFALHNDSIDQKCSRLECLELDAPLPLYMQRSWDIIN 470

[Close]

BLAST of MS012222 vs. NCBI nr
Match: XP_023553811.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.4 bits (2084), Expect = 6.7e-230
Identity = 393/472 (83.26%), Postives = 429/472 (90.89%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LPA+LRRS+I    PSS A GG SLHFS+F E KSTFALSN +ET+SE+KNE KG NR
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TATV+GS LGNRI+N G+QAE ETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATVNGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SECNLEA++QLKRVAA DILN+GD++ LPISVQVFP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KF+HSLVLYKDPAI+ INKPPGLPVQGGIG+ RSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTSVLHSIFREKT GASKH  HVD EKKILQK+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKKILQKRYWALVIGSPRRTKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPL K+VLDDGRSE+IMV+DNA+SM FQ AITTY+VIK+HHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVVDNAESMPFQHAITTYKVIKTHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNW+PFP N HKNQGKKQSLPFGLDFE+GS+AEN P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENNP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           RLHLHCK L LP+VS AL +DSLDHD S LE LELDAPLPSYM+RSWDIINN
Sbjct: 421 RLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of MS012222 vs. NCBI nr
Match: KAG7011636.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 807.0 bits (2083), Expect = 8.7e-230
Identity = 393/472 (83.26%), Postives = 430/472 (91.10%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+ PA+LRRS+I    PSS A GG SLHFS+F E KSTFALS+ +ET+SE+KNE KG NR
Sbjct: 1   MVFPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNRI+N G+QAE ETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SECNLEA++QLKRVAA DILN+GD++ LPISVQVFP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KF+HSLVLYKDPAI+ INKPPGLPVQGGIG+ RSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTSVLHSIFREKT GASKH  HVDSEKKILQK+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPL K+VLDDGRSE+IMVIDNA+SM FQ AITTY+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNW+PFP N HKNQGKKQSLPFGLDFE+GS+AEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           RLHLHCKQL LP+VS AL +DSLDHD S LE LELDAPLPSYM+RSWDIINN
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of MS012222 vs. NCBI nr
Match: XP_022952780.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-229
Identity = 393/472 (83.26%), Postives = 430/472 (91.10%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LPA+LRRS+I    PSS A GG SLHFS+F E KST+ALS  +ET+SE+KNE KG NR
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNRI+N G+QAE ETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SECNLEA++QLKRVAA DILN+GD++ LPISVQVFP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KF+HSLVLYKDPAI+ INKPPGLPVQGGIG+ RSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTSVLHSIFREKT GASKH  HVDSEKKILQK+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPL K+VLDDGRSE+IMVIDNA+SM FQ AITTY+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNW+PFP N HKNQGKKQSLPFGLDFE+GS+AEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           RLHLHCKQL LP+VS AL +DSLDHD S LE LELDAPLPSYM+RSWDIINN
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of MS012222 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 7.1e-134
Identity = 254/478 (53.14%), Postives = 327/478 (68.41%), Query Frame = 0


Query: 2   LLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNRW 61
           L  A LRR L  S    ST +       +  H+   +++ + T ++            +W
Sbjct: 6   LATATLRRQLQSSSPTISTFKNPTKALSAAAHQSTRSYSTTQTDDS----------RGKW 65

Query: 62  FTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRLR 121
            TLPP++ T+DG+ +G  +L+ G   +  T  +  TAL+W+LRC P+LPR+LVQKLFRLR
Sbjct: 66  LTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLR 125

Query: 122 KIRRGSSM-IESSECNLEAQNQLKRVAAKDILNVGDQIFLPISV-----QVFPRERARDY 181
           ++RR  SM ++  E     ++QLKRVAAK+ LNVGD+I+LP+SV     Q  P ++    
Sbjct: 126 QVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIYLPLSVDNDTPQTPPAKKESFQ 185

Query: 182 CNKEEEKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLV 241
           C+ EE KF+ SLVLYKDPAIIV+NKP GL VQGG GI  S+DELAASCL +D SE PRLV
Sbjct: 186 CSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLV 245

Query: 242 HRLDRDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRC 301
           HRLDRD SG+LV+ RT T+ +VLHSIFREKT GAS + V    K L++KY ALVIG P  
Sbjct: 246 HRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVKSLKRKYMALVIGCPPR 305

Query: 302 PNGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSH-HGYTWLELCPLTG 361
             G I+APL K+V+DDG+SE+I V DN + ++ Q AIT YRVI+S  HGYTWLEL PLTG
Sbjct: 306 QRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTG 365

Query: 362 RKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVA 421
           RKHQLRVHCAEVLGTPIVGDYKYGWQAH+  EPF S+   N  K+ S PFGLD + G V+
Sbjct: 366 RKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS-ENNPTKQSSSPFGLDLDGGDVS 425

Query: 422 ENTPRLHLHCKQLALPNVSGALP--SDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
              P LHLH KQ+ LPN+S  L     S D D S+L+ L+ DAPLPS+M+ S++++ +
Sbjct: 426 SKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of MS012222 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.1e-81
Identity = 165/427 (38.64%), Postives = 257/427 (60.19%), Query Frame = 0


Query: 63  TLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRLRK 122
           +LPP+    DG  L  R            +   +TA+ WV     ++P+ +VQ  F  R 
Sbjct: 55  SLPPFPGGGDGDPLPGR---------KAAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRL 114

Query: 123 IRRGSSMIESSECNLEAQ-NQLKRVAAKDILNVGDQIFLPISV---QVFPR----ERARD 182
           +    S  E S   +++Q + LK++   D++  G +I LP+SV   ++  R      A  
Sbjct: 115 VYSECSDHEVSVETIKSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATL 174

Query: 183 YCNKEEEKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRL 242
           + NK+E +++  LV++KD AI+V+NKPP +P++G + +  S+D LAA+ LSY   E P+L
Sbjct: 175 HPNKDEIEYLRRLVIHKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKL 234

Query: 243 VHRLDRDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHV-DSEKKILQKKYWALVIGSP 302
           VHRLDR+SSG+L+ GRT  S + LH +F       +   V ++  +   ++YWALVIG+P
Sbjct: 235 VHRLDRESSGLLLFGRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTP 294

Query: 303 RCPNGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVI-KSHHGYTWLELCPL 362
           +   G+I+APL K++LDDG++E++++   +     Q+A+T YRV+  + HG +W+EL PL
Sbjct: 295 KEREGIISAPLSKVLLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPL 354

Query: 363 TGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPS-NFHKNQGKKQSL--PFGLDFE 422
           TGRKHQLRVHCAE LGTPIVGDYKYGW  H+ W+  P  +F    G+   L  P GL+ +
Sbjct: 355 TGRKHQLRVHCAEALGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQ 414

Query: 423 NGSVAENTPRLHLHCKQLALPNVSGALPSDSLDHD------FSELECLELDAPLPSYMRR 471
            GSV    P LHLHC+++ +PN++  L S+   H+        +   L   AP+P++M+ 
Sbjct: 415 KGSVLSKVPLLHLHCREMVIPNIAKFLSSNGEWHENGAPWSKEKPNLLRFIAPMPAHMKI 472

[Close]

BLAST of MS012222 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 3.8e-79
Identity = 162/417 (38.85%), Postives = 244/417 (58.51%), Query Frame = 0


Query: 82  NQGSQAEDETSTTAITALKWVLRCCPEL---------PRSLVQKLFRLRKIRRGSSMIES 141
           N G+  +D  S   +TA+ WV     E+          + LVQ  FR R+        ++
Sbjct: 67  NAGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DA 126

Query: 142 SECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARD-------YCNKEEEKFMH 201
           S    +    ++++   +++ +GD+I+LP+S+      +  D       Y N +E  ++ 
Sbjct: 127 SREKEDGAIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQ 186

Query: 202 SLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDRDSSGI 261
            LV +KD AIIV+NKPP LPV+G + I  S+D LAA+ LS+   E PRLVHRLDR++SG+
Sbjct: 187 RLVRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGL 246

Query: 262 LVMGRTSTSTSVLHSIFRE-KTFGASKHHVDSEKKILQKKYWALVIGSPRCPNGLITAPL 321
           LVMGRT  S   LHS+F + K   +S    +   + + ++YWALVIGSP+   GLI+APL
Sbjct: 247 LVMGRTKESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPL 306

Query: 322 GKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVI-KSHHGYTWLELCPLTGRKHQLRVHC 381
            K++LDDG+++++++   +   A Q AIT Y+V+    +G +W+EL P+T RKHQLRVHC
Sbjct: 307 SKVLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHC 366

Query: 382 AEVLGTPIVGDYKYGWQAHRNWEPFPS-NFHKNQGKKQSL--PFGLDFENGSVAENTPRL 441
           AE LGTPIVGDYKYGW  H+ W+  P  +     GK   L  P GLD + GSV    P L
Sbjct: 367 AEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLL 426

Query: 442 HLHCKQLALPNVSGALPSDSLDHD-------FSELECLELDAPLPSYMRRSWDIINN 471
           HLHC+++ LPN++  L   +             + + L   A +PS+M+ SW+++++
Sbjct: 427 HLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

[Close]

BLAST of MS012222 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.6e-77
Identity = 166/357 (46.50%), Postives = 224/357 (62.75%), Query Frame = 0


Query: 55  KKGFNRWFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLV 114
           ++G + W  LPP+ A +D +     I   G +  D       TA+KWV RCCP+LP SLV
Sbjct: 46  ERGKSPWLQLPPF-APLDAAAAARAISRGGGEGGD--GEQGATAIKWVRRCCPDLPTSLV 105

Query: 115 QKLFRLRKIRRGSSMIESSECNLEA-QNQLKRVAAKDILNVGDQIFLPISV-QVFPRERA 174
           QKLFRLRK+++     E S  +  A Q++L+RV+AKD L  GD +FLP+++ +    E+ 
Sbjct: 106 QKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVAEKT 165

Query: 175 RDYCNKEEEKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPP 234
           + + N+ E  F+  L +YKD AIIV+NKPPG+PVQGG+GI  S+D L AS    + SE P
Sbjct: 166 KKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSSEAP 225

Query: 235 RLVHRLDRDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGS 294
           RLVHRLDRD SGILV+GR   STS+LH+IFREKT  A     D  + +LQ+KY ALVIG+
Sbjct: 226 RLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTADAL---ADGTQHVLQRKYVALVIGT 285

Query: 295 PRCPNGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKS-HHGYTWLELCP 354
           PR P GL++APL KI+L DG+SE++ +  ++ + + Q A+T YRVI+S   GYTWLEL P
Sbjct: 286 PRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTWLELFP 345

Query: 355 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFP--SNFHKNQGKKQSLPFGL 407
            TGRKHQ        L   ++G      +  R+   FP      +N   K S P GL
Sbjct: 346 RTGRKHQ------RFLEPQLLGITSMDGKRIRSGCLFPCHGQLMRNCSGKGSFPLGL 389

[Close]

BLAST of MS012222 vs. ExPASy Swiss-Prot
Match: Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-28
Identity = 88/302 (29.14%), Postives = 142/302 (47.02%), Query Frame = 0


Query: 184 MHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDY---SEPPRLVHRLDR 243
           + S V+ +DP ++++NKP GLPV GG  + RS+  L  +   + +   +EP +L HRLDR
Sbjct: 47  LKSQVVREDPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAEPLKLCHRLDR 106

Query: 244 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRCPNGLI 303
           D++G L++ RT+ +   +    RE+               + + YWAL +G+P    G++
Sbjct: 107 DTTGALILARTTEAAERVQQALRERE--------------VHRVYWALCLGTPSPREGIL 166

Query: 304 TAPLGK------------------IVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHH 363
             P+ +                   V ++G  E++ V  +A      +A+T YR + +  
Sbjct: 167 DIPIMEKETSGPQKHYKMALSPRFRVSEEGAVERVRVPRSA-----HEAVTRYRTLGAAS 226

Query: 364 GYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSL 423
           G + +EL P+TG KHQLRVH A  L  PI+GD+KY        +  P +  +  G   ++
Sbjct: 227 GASLVELHPITGVKHQLRVHLALGLNCPILGDHKYSHWGRLAPQKPPDSVLRALG--LTV 286

Query: 424 PFGLDFENGSVAENTPRLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMR 465
           P             T  LHLH  QL LP+  G+ P             + L  PLP   R
Sbjct: 287 P----------QARTLSLHLHAVQLTLPSSDGSTP-------------IVLQCPLPYTFR 304

[Close]

BLAST of MS012222 vs. ExPASy TrEMBL
Match: A0A6J1D432 (RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017405 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 2.8e-274
Identity = 466/470 (99.15%), Postives = 469/470 (99.79%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRCPNGLI 300
           DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPR PNGLI
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRRPNGLI 300

Query: 301 TAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR 360
           TAPLGKIVLDDGRSEKIMVIDNA+SMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR
Sbjct: 301 TAPLGKIVLDDGRSEKIMVIDNAESMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQLR 360

Query: 361 VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTPRL 420
           VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAEN+PRL
Sbjct: 361 VHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENSPRL 420

Query: 421 HLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           HLHCKQLALPNVSGALP+DSLDHDFSELECLELDAPLPSYMRRSWDIINN
Sbjct: 421 HLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

[Close]

BLAST of MS012222 vs. ExPASy TrEMBL
Match: A0A6J1GMP9 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455375 PE=4 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 9.4e-230
Identity = 393/472 (83.26%), Postives = 430/472 (91.10%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LPA+LRRS+I    PSS A GG SLHFS+F E KST+ALS  +ET+SE+KNE KG NR
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TAT +GS LGNRI+N G+QAE ETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SECNLEA++QLKRVAA DILN+GD++ LPISVQVFP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KF+HSLVLYKDPAI+ INKPPGLPVQGGIG+ RSLD+LAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTSVLHSIFREKT GASKH  HVDSEKKILQK+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDSEKKILQKRYWALVIGSPRRTKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPL K+VLDDGRSE+IMVIDNA+SM FQ AITTY+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVIDNAESMPFQHAITTYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAHRNW+PFP N HKNQGKKQSLPFGLDFE+GS+AEN+P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESGSIAENSP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           RLHLHCKQL LP+VS AL +DSLDHD S LE LELDAPLPSYM+RSWDIINN
Sbjct: 421 RLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of MS012222 vs. ExPASy TrEMBL
Match: A0A6J1IBC5 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470950 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.8e-225
Identity = 389/473 (82.24%), Postives = 425/473 (89.85%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LPALLRRS+I    PSS A GG SLHFS+F E KSTFALSN +ET+SE+KNE KG NR
Sbjct: 1   MVLPALLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPP+TATV+GS LGNRI+N G+Q+E ETSTTAITALKWV RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPFTATVNGSALGNRIMNGGAQSEAETSTTAITALKWVFRCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SECNLEA++QLKRVAA DILN+GD++ LPISVQVFP ERA D CNKEE
Sbjct: 121 RKVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           +KF+HSLVLYKDPAI+ INKPPGLPVQGGIG+ RSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 KKFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTSVLHSIFREKT GASKH  HVD EK ILQK+YWALVIGSP    G
Sbjct: 241 DSSGILIMGRTSTSTSVLHSIFREKTLGASKHANHVDREKMILQKRYWALVIGSPSHTKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKS-MAFQQAITTYRVIKSHHGYTWLELCPLTGRKH 360
           LITAPL K+VLDDGRSE+IMV+DNA+S M FQ AITTY+VIKSHHGYTW+ELCPLTGRKH
Sbjct: 301 LITAPLVKMVLDDGRSEQIMVVDNAESIMPFQHAITTYKVIKSHHGYTWVELCPLTGRKH 360

Query: 361 QLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENT 420
           QLRVHCAE LGTPIVGDYKYGWQAHRN +PFP N HKNQGKKQSLPFGLDFE+GS+AEN 
Sbjct: 361 QLRVHCAEALGTPIVGDYKYGWQAHRNLKPFPHNSHKNQGKKQSLPFGLDFESGSIAENN 420

Query: 421 PRLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           PRLHLHCK L LP+VS AL +DSLDHD S LE LELDAPLPSYM+RSWDIINN
Sbjct: 421 PRLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 472

[Close]

BLAST of MS012222 vs. ExPASy TrEMBL
Match: A0A5D3C4F5 (RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00930 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.4e-224
Identity = 384/472 (81.36%), Postives = 421/472 (89.19%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LP L R S+IL  +PS T      L+FS+FH LKST ALSN SE +S++KNE KG NR
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV+ S+LGNRILN+G+QAE ETST AITALKWV+ CCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SE NLEA++QLKRVAAKDILNVGDQIFLPIS QVF  E+ RDYCNK+E
Sbjct: 121 RKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           E FMHSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSY YSEPPRLVHRLDR
Sbjct: 181 ETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTS+LHS+FREKTF ASKH  HVDSEKKIL K+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPLGKIVLDDGRSE+IMVIDNA+ M FQQAIT Y+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAH+NW+PF  NFHKNQGKKQ LPFGLDFE+GS+AE  P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           RLHLHCKQL LP+VS AL +DSLD D S LECLELDAPLPSYM++SWDIIN+
Sbjct: 421 RLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]

BLAST of MS012222 vs. ExPASy TrEMBL
Match: A0A1S3BZE7 (RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495275 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.4e-224
Identity = 384/472 (81.36%), Postives = 421/472 (89.19%), Query Frame = 0


Query: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60
           M+LP L R S+IL  +PS T      L+FS+FH LKST ALSN SE +S++KNE KG NR
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNE-KGSNR 60

Query: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120
           WFTLPPYTATV+ S+LGNRILN+G+QAE ETST AITALKWV+ CCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRL 120

Query: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180
           RK+RRGSSMIE+SE NLEA++QLKRVAAKDILNVGDQIFLPIS QVF  E+ RDYCNK+E
Sbjct: 121 RKVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDE 180

Query: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240
           E FMHSLVLYKDPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSY YSEPPRLVHRLDR
Sbjct: 181 ETFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDR 240

Query: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--HVDSEKKILQKKYWALVIGSPRCPNG 300
           DSSGIL+MGRTSTSTS+LHS+FREKTF ASKH  HVDSEKKIL K+YWALVIGSPR   G
Sbjct: 241 DSSGILIMGRTSTSTSLLHSVFREKTFDASKHANHVDSEKKILLKRYWALVIGSPRRSKG 300

Query: 301 LITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRKHQ 360
           LITAPLGKIVLDDGRSE+IMVIDNA+ M FQQAIT Y+VIKSHHGYTWLELCPLTGRKHQ
Sbjct: 301 LITAPLGKIVLDDGRSERIMVIDNAEPMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQ 360

Query: 361 LRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVAENTP 420
           LRVHCAEVLGTPIVGDYKYGWQAH+NW+PF  NFHKNQGKKQ LPFGLDFE+GS+AE  P
Sbjct: 361 LRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESGSIAETNP 420

Query: 421 RLHLHCKQLALPNVSGALPSDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
           RLHLHCKQL LP+VS AL +DSLD D S LECLELDAPLPSYM++SWDIIN+
Sbjct: 421 RLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]

BLAST of MS012222 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 478.8 bits (1231), Expect = 5.0e-135
Identity = 254/478 (53.14%), Postives = 327/478 (68.41%), Query Frame = 0


Query: 2   LLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNRW 61
           L  A LRR L  S    ST +       +  H+   +++ + T ++            +W
Sbjct: 6   LATATLRRQLQSSSPTISTFKNPTKALSAAAHQSTRSYSTTQTDDS----------RGKW 65

Query: 62  FTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRLR 121
            TLPP++ T+DG+ +G  +L+ G   +  T  +  TAL+W+LRC P+LPR+LVQKLFRLR
Sbjct: 66  LTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLR 125

Query: 122 KIRRGSSM-IESSECNLEAQNQLKRVAAKDILNVGDQIFLPISV-----QVFPRERARDY 181
           ++RR  SM ++  E     ++QLKRVAAK+ LNVGD+I+LP+SV     Q  P ++    
Sbjct: 126 QVRREMSMSVDGDELQ---RSQLKRVAAKESLNVGDRIYLPLSVDNDTPQTPPAKKESFQ 185

Query: 182 CNKEEEKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLV 241
           C+ EE KF+ SLVLYKDPAIIV+NKP GL VQGG GI  S+DELAASCL +D SE PRLV
Sbjct: 186 CSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLV 245

Query: 242 HRLDRDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSEKKILQKKYWALVIGSPRC 301
           HRLDRD SG+LV+ RT T+ +VLHSIFREKT GAS + V    K L++KY ALVIG P  
Sbjct: 246 HRLDRDCSGLLVLARTQTAATVLHSIFREKTTGASAYGVKKNVKSLKRKYMALVIGCPPR 305

Query: 302 PNGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSH-HGYTWLELCPLTG 361
             G I+APL K+V+DDG+SE+I V DN + ++ Q AIT YRVI+S  HGYTWLEL PLTG
Sbjct: 306 QRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTG 365

Query: 362 RKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFENGSVA 421
           RKHQLRVHCAEVLGTPIVGDYKYGWQAH+  EPF S+   N  K+ S PFGLD + G V+
Sbjct: 366 RKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS-ENNPTKQSSSPFGLDLDGGDVS 425

Query: 422 ENTPRLHLHCKQLALPNVSGALP--SDSLDHDFSELECLELDAPLPSYMRRSWDIINN 471
              P LHLH KQ+ LPN+S  L     S D D S+L+ L+ DAPLPS+M+ S++++ +
Sbjct: 426 SKQPHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of MS012222 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.7e-80
Identity = 162/417 (38.85%), Postives = 244/417 (58.51%), Query Frame = 0


Query: 82  NQGSQAEDETSTTAITALKWVLRCCPEL---------PRSLVQKLFRLRKIRRGSSMIES 141
           N G+  +D  S   +TA+ WV     E+          + LVQ  FR R+        ++
Sbjct: 67  NAGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRR--------DA 126

Query: 142 SECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARD-------YCNKEEEKFMH 201
           S    +    ++++   +++ +GD+I+LP+S+      +  D       Y N +E  ++ 
Sbjct: 127 SREKEDGAIPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQ 186

Query: 202 SLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDRDSSGI 261
            LV +KD AIIV+NKPP LPV+G + I  S+D LAA+ LS+   E PRLVHRLDR++SG+
Sbjct: 187 RLVRFKDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGL 246

Query: 262 LVMGRTSTSTSVLHSIFRE-KTFGASKHHVDSEKKILQKKYWALVIGSPRCPNGLITAPL 321
           LVMGRT  S   LHS+F + K   +S    +   + + ++YWALVIGSP+   GLI+APL
Sbjct: 247 LVMGRTKESIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPL 306

Query: 322 GKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVI-KSHHGYTWLELCPLTGRKHQLRVHC 381
            K++LDDG+++++++   +   A Q AIT Y+V+    +G +W+EL P+T RKHQLRVHC
Sbjct: 307 SKVLLDDGKTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHC 366

Query: 382 AEVLGTPIVGDYKYGWQAHRNWEPFPS-NFHKNQGKKQSL--PFGLDFENGSVAENTPRL 441
           AE LGTPIVGDYKYGW  H+ W+  P  +     GK   L  P GLD + GSV    P L
Sbjct: 367 AEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLL 426

Query: 442 HLHCKQLALPNVSGALPSDSLDHD-------FSELECLELDAPLPSYMRRSWDIINN 471
           HLHC+++ LPN++  L   +             + + L   A +PS+M+ SW+++++
Sbjct: 427 HLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSS 475

[Close]

BLAST of MS012222 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 74.3 bits (181), Expect = 2.9e-13
Identity = 82/344 (23.84%), Postives = 139/344 (40.41%), Query Frame = 0


Query: 64  LPPYTATVDGSVLGNRILNQGSQAED--ETSTTAITALKWVLRCCPELPRSLVQKLFRLR 123
           +P   A     ++  R+ N G + E+  + +   I    W+      + R+ VQ   RL 
Sbjct: 48  IPNLVACSPTEIIIPRVNNAGLRIEEIVDAAKGKIRLDSWISSRINGVSRARVQSSIRLG 107

Query: 124 KIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEEE 183
            +     +++    N+++               GD++   IS     +  A D       
Sbjct: 108 LVTVNGRVVDKVSHNVKS---------------GDEVNCTISELQPLKAEAEDIPLDIVY 167

Query: 184 KFMHSLVLYKDPAIIVINKPPGLP---VQGGIGITRSL---------------------- 243
           +  H LV+ K PA +V++  PG P   +  GI    SL                      
Sbjct: 168 EDKHVLVVNK-PAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDD 227

Query: 244 DELAASCLSYDYSEPPRLVHRLDRDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDS 303
           +E+  S  SY  S  P +VHRLD+ ++G+LV+ +   S + L   F+  T          
Sbjct: 228 EEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHT---------- 287

Query: 304 EKKILQKKYWALVIGSPRCPNGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYR 363
               +++ Y +L  G P  P G I  P+G+      R     +    +    + A + Y+
Sbjct: 288 ----IERVYVSLTTGVPSPPQGRIEIPIGRD--SSNRIRMAAIPGGVRGGRARHAASRYK 347

Query: 364 VIKSH--HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 379
           VI++    G   +E    TGR HQ+R H A+ +G P++GD  YG
Sbjct: 348 VIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYG 358

[Close]

BLAST of MS012222 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 72.8 bits (177), Expect = 8.3e-13
Identity = 62/201 (30.85%), Postives = 94/201 (46.77%), Query Frame = 0


Query: 188 VLYKDPAIIVINKPPGLPV-QGGIGITRSLDELAASCLSYD-------YSEPPRLVHRLD 247
           VLY+D  +I +NKP GL V  GG+   R++      C   +        S  P  VHRL 
Sbjct: 99  VLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLG 158

Query: 248 RDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSE---KKILQKKYWALVIGSPRCP 307
           R +SGIL+  +T  + + L + F E T      ++D E    + L K Y AL  G     
Sbjct: 159 RGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEED 218

Query: 308 NGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRK 367
             +I  P+G +V   G ++ + V       AF +     R  + +     +E+   +GR 
Sbjct: 219 EVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEI--QSGRP 278

Query: 368 HQLRVHCAEVLGTPIVGDYKY 378
           HQ+R+H A  +G P+VGD  Y
Sbjct: 279 HQIRIHLA-YMGHPLVGDPLY 295

[Close]

BLAST of MS012222 vs. TAIR 10
Match: AT3G52260.3 (Pseudouridine synthase family protein )

HSP 1 Score: 67.4 bits (163), Expect = 3.5e-11
Identity = 59/196 (30.10%), Postives = 91/196 (46.43%), Query Frame = 0


Query: 188 VLYKDPAIIVINKPPGLPV-QGGIGITRSLDELAASCLSYD-------YSEPPRLVHRLD 247
           VLY+D  +I +NKP GL V  GG+   R++      C   +        S  P  VHRL 
Sbjct: 129 VLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLG 188

Query: 248 RDSSGILVMGRTSTSTSVLHSIFREKTFGASKHHVDSE---KKILQKKYWALVIGSPRCP 307
           R +SGIL+  +T  + + L + F E T      ++D E    + L K Y AL  G     
Sbjct: 189 RGTSGILLCAKTKLAKTKLAAYFAEGTSLVGSGNLDQECGTGRKLSKIYRALADGIVEED 248

Query: 308 NGLITAPLGKIVLDDGRSEKIMVIDNAKSMAFQQAITTYRVIKSHHGYTWLELCPLTGRK 367
             +I  P+G +V   G ++ + V       AF +     R  + +     +E+   +GR 
Sbjct: 249 EVVIKQPIG-VVRYPGVAQGLYVASPEGKPAFSKVFVLERDREKNCSLVKVEI--QSGRP 308

Query: 368 HQLRVHCAEVLGTPIV 373
           HQ+R+H A  +G P+V
Sbjct: 309 HQIRIHLA-YMGHPLV 320

The following BLAST results are available for this feature:

BLAST of MS012222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148845.1	5.7e-274	99.15	RNA pseudouridine synthase 4, mitochondrial [Momordica charantia]	[more]
XP_038888757.1	1.3e-230	83.44	RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida]	[more]
XP_023553811.1	6.7e-230	83.26	RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
KAG7011636.1	8.7e-230	83.26	RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma sub...	[more]
XP_022952780.1	1.9e-229	83.26	RNA pseudouridine synthase 4, mitochondrial [Cucurbita moschata]	[more]

BLAST of MS012222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT72	7.1e-134	53.14	RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q0J4D4	3.1e-81	38.64	RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3...	[more]
Q5XET6	3.8e-79	38.85	RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q69K07	3.6e-77	46.50	RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3...	[more]
Q28C59	1.1e-28	29.14	Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN...	[more]

BLAST of MS012222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D432	2.8e-274	99.15	RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1GMP9	9.4e-230	83.26	RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IBC5	1.8e-225	82.24	RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A5D3C4F5	3.4e-224	81.36	RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BZE7	3.4e-224	81.36	RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495...	[more]

BLAST of MS012222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19440.1	5.0e-135	53.14	Pseudouridine synthase family protein	[more]
AT1G78910.1	2.7e-80	38.85	Pseudouridine synthase family protein	[more]
AT1G76050.2	2.9e-13	23.84	Pseudouridine synthase family protein	[more]
AT3G52260.2	8.3e-13	30.85	Pseudouridine synthase family protein	[more]
AT3G52260.3	3.5e-11	30.10	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 195..364 e-value: 3.2E-25 score: 89.1
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 195..429 e-value: 9.03288E-53 score: 174.063
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 176..436 e-value: 7.6E-53 score: 181.3
None	No IPR available	PANTHER	PTHR21600:SF53	RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIAL	coord: 50..468
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 50..468
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 183..466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012222.1	MS012222.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding