MS012219 (gene) Bitter gourd (TR) v1

Overview
NameMS012219
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCCHC-type domain-containing protein
Locationscaffold797: 525736 .. 525981 (-)
RNA-Seq ExpressionMS012219
SyntenyMS012219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCGGCAATGCTATTGGGGTGTGTGAGGATGTTGACTGTGATTCTGACGAGTATTGTTTGGGGGAAAGTCTGACTGTCAAAGTGAGATTTGACATAACAAAACCCTTGAGACGAGGTGTGAAGATCAATATTGATGGTCCTATGGGTAGCTGTTGGGTTCCAATCAAGTATGAGCGTTTACCAGATTTTTGTTATTTCTGTGGGATGATTGGCCATCTAGCTAAAACTTGTGATTCAAATGTTGAT

mRNA sequence

CTCGGCAATGCTATTGGGGTGTGTGAGGATGTTGACTGTGATTCTGACGAGTATTGTTTGGGGGAAAGTCTGACTGTCAAAGTGAGATTTGACATAACAAAACCCTTGAGACGAGGTGTGAAGATCAATATTGATGGTCCTATGGGTAGCTGTTGGGTTCCAATCAAGTATGAGCGTTTACCAGATTTTTGTTATTTCTGTGGGATGATTGGCCATCTAGCTAAAACTTGTGATTCAAATGTTGAT

Coding sequence (CDS)

CTCGGCAATGCTATTGGGGTGTGTGAGGATGTTGACTGTGATTCTGACGAGTATTGTTTGGGGGAAAGTCTGACTGTCAAAGTGAGATTTGACATAACAAAACCCTTGAGACGAGGTGTGAAGATCAATATTGATGGTCCTATGGGTAGCTGTTGGGTTCCAATCAAGTATGAGCGTTTACCAGATTTTTGTTATTTCTGTGGGATGATTGGCCATCTAGCTAAAACTTGTGATTCAAATGTTGAT

Protein sequence

LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERLPDFCYFCGMIGHLAKTCDSNVD
Homology
BLAST of MS012219 vs. NCBI nr
Match: XP_022156185.1 (uncharacterized protein LOC111023135 [Momordica charantia] >XP_022156186.1 uncharacterized protein LOC111023135 [Momordica charantia])

HSP 1 Score: 127.9 bits (320), Expect = 4.1e-26
Identity = 53/79 (67.09%), Postives = 63/79 (79.75%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           LGNAIG   DVDC+   +  G SL ++V  DITKPLRRG+KINIDGPMG CW+PI+YERL
Sbjct: 148 LGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPLRRGIKINIDGPMGGCWIPIQYERL 207

Query: 61  PDFCYFCGMIGHLAKTCDS 80
           PDFCYFCG+IGH +  CD+
Sbjct: 208 PDFCYFCGVIGHSSHDCDA 226

BLAST of MS012219 vs. NCBI nr
Match: XP_022132681.1 (uncharacterized protein LOC111005481 [Momordica charantia])

HSP 1 Score: 119.0 bits (297), Expect = 1.9e-23
Identity = 46/77 (59.74%), Postives = 61/77 (79.22%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           LGNAIG+ EDV+ +++ +C G  L V+VRFD+ KPL RG+K+N+DGPMG CW+PI+YERL
Sbjct: 149 LGNAIGLFEDVESNANNFCWGSCLRVRVRFDVMKPLHRGIKLNLDGPMGGCWIPIQYERL 208

Query: 61  PDFCYFCGMIGHLAKTC 78
           PDF Y CG + H+ K C
Sbjct: 209 PDFRYHCGRLDHILKDC 225

BLAST of MS012219 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 101.7 bits (252), Expect = 3.1e-18
Identity = 41/69 (59.42%), Postives = 53/69 (76.81%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           LGNA+G  E+ DCD      G +L V+V  DI+KPLRRG+K+N+DGP+G  W+PI+YERL
Sbjct: 149 LGNALGGFEEADCDDLNPDWGSNLRVRVMLDISKPLRRGIKLNLDGPIGGAWIPIQYERL 208

Query: 61  PDFCYFCGM 70
           PDFCY CG+
Sbjct: 209 PDFCYHCGL 217

BLAST of MS012219 vs. NCBI nr
Match: XP_042958115.1 (uncharacterized protein LOC122293643 [Carya illinoinensis])

HSP 1 Score: 94.0 bits (232), Expect = 6.6e-16
Identity = 40/77 (51.95%), Postives = 52/77 (67.53%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           +GN+IGV E++D + DE   GE + +++  D+TKPL RG  IN+ G  GSCWV   YERL
Sbjct: 124 IGNSIGVVEEIDVEKDEVAWGEFIKIRILLDVTKPLLRGKCINL-GNHGSCWVRFTYERL 183

Query: 61  PDFCYFCGMIGHLAKTC 78
           P FCY CG +GH  K C
Sbjct: 184 PTFCYGCGRLGHSHKDC 199

BLAST of MS012219 vs. NCBI nr
Match: XP_035547259.1 (uncharacterized protein LOC118348856 [Juglans regia])

HSP 1 Score: 91.3 bits (225), Expect = 4.2e-15
Identity = 37/80 (46.25%), Postives = 53/80 (66.25%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           +GN IG+ E+VD +  E   GE L ++VR +I++PL RG+ + + G  GS W+   YERL
Sbjct: 120 IGNKIGIVEEVDGEQSEVAWGEYLRIRVRVNISQPLMRGITVKL-GQFGSVWIRFAYERL 179

Query: 61  PDFCYFCGMIGHLAKTCDSN 81
           P+FCY CG +GH  K C+ N
Sbjct: 180 PNFCYMCGKLGHQQKDCEIN 198

BLAST of MS012219 vs. ExPASy TrEMBL
Match: A0A6J1DU55 (uncharacterized protein LOC111023135 OS=Momordica charantia OX=3673 GN=LOC111023135 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.0e-26
Identity = 53/79 (67.09%), Postives = 63/79 (79.75%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           LGNAIG   DVDC+   +  G SL ++V  DITKPLRRG+KINIDGPMG CW+PI+YERL
Sbjct: 148 LGNAIGNFVDVDCNEKGFSWGASLRIRVLIDITKPLRRGIKINIDGPMGGCWIPIQYERL 207

Query: 61  PDFCYFCGMIGHLAKTCDS 80
           PDFCYFCG+IGH +  CD+
Sbjct: 208 PDFCYFCGVIGHSSHDCDA 226

BLAST of MS012219 vs. ExPASy TrEMBL
Match: A0A6J1BSZ1 (uncharacterized protein LOC111005481 OS=Momordica charantia OX=3673 GN=LOC111005481 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 9.2e-24
Identity = 46/77 (59.74%), Postives = 61/77 (79.22%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           LGNAIG+ EDV+ +++ +C G  L V+VRFD+ KPL RG+K+N+DGPMG CW+PI+YERL
Sbjct: 149 LGNAIGLFEDVESNANNFCWGSCLRVRVRFDVMKPLHRGIKLNLDGPMGGCWIPIQYERL 208

Query: 61  PDFCYFCGMIGHLAKTC 78
           PDF Y CG + H+ K C
Sbjct: 209 PDFRYHCGRLDHILKDC 225

BLAST of MS012219 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.5e-18
Identity = 41/69 (59.42%), Postives = 53/69 (76.81%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           LGNA+G  E+ DCD      G +L V+V  DI+KPLRRG+K+N+DGP+G  W+PI+YERL
Sbjct: 149 LGNALGGFEEADCDDLNPDWGSNLRVRVMLDISKPLRRGIKLNLDGPIGGAWIPIQYERL 208

Query: 61  PDFCYFCGM 70
           PDFCY CG+
Sbjct: 209 PDFCYHCGL 217

BLAST of MS012219 vs. ExPASy TrEMBL
Match: A0A6P9EID8 (uncharacterized protein LOC118348856 OS=Juglans regia OX=51240 GN=LOC118348856 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.1e-15
Identity = 37/80 (46.25%), Postives = 53/80 (66.25%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           +GN IG+ E+VD +  E   GE L ++VR +I++PL RG+ + + G  GS W+   YERL
Sbjct: 120 IGNKIGIVEEVDGEQSEVAWGEYLRIRVRVNISQPLMRGITVKL-GQFGSVWIRFAYERL 179

Query: 61  PDFCYFCGMIGHLAKTCDSN 81
           P+FCY CG +GH  K C+ N
Sbjct: 180 PNFCYMCGKLGHQQKDCEIN 198

BLAST of MS012219 vs. ExPASy TrEMBL
Match: K3ZNG2 (CCHC-type domain-containing protein OS=Setaria italica OX=4555 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.5e-15
Identity = 37/77 (48.05%), Postives = 52/77 (67.53%), Query Frame = 0

Query: 1   LGNAIGVCEDVDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERL 60
           +G+  G   +VD +SD+   G  L VKVR DI  PLRRG+ +++   +G  W P++YE L
Sbjct: 144 IGDEFGKFLEVDFESDDLAAGRVLRVKVRLDIRLPLRRGITVDLGEGVGDRWCPVQYEFL 203

Query: 61  PDFCYFCGMIGHLAKTC 78
           P+FCY CG+IGH+ KTC
Sbjct: 204 PEFCYVCGIIGHVDKTC 220

BLAST of MS012219 vs. TAIR 10
Match: AT2G01050.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 43.1 bits (100), Expect = 1.2e-04
Identity = 23/71 (32.39%), Postives = 33/71 (46.48%), Query Frame = 0

Query: 11  VDCDSDEYCLGESLTVKVRFDITKPLRRGVKINIDGPMGSCWVPIKYERLPDFCYFCGMI 70
           VD ++  +  G    V +  ++ KPL+  V IN D         + YE L   C  CG+ 
Sbjct: 196 VDMNTINFDKGRFARVCIEVNLAKPLKGTVLINGDRYF------VAYEGLSKICSSCGIY 255

Query: 71  GHLAKTCDSNV 82
           GHL  +C  NV
Sbjct: 256 GHLVHSCPRNV 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156185.14.1e-2667.09uncharacterized protein LOC111023135 [Momordica charantia] >XP_022156186.1 uncha... [more]
XP_022132681.11.9e-2359.74uncharacterized protein LOC111005481 [Momordica charantia][more]
XP_022158377.13.1e-1859.42uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_042958115.16.6e-1651.95uncharacterized protein LOC122293643 [Carya illinoinensis][more]
XP_035547259.14.2e-1546.25uncharacterized protein LOC118348856 [Juglans regia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DU552.0e-2667.09uncharacterized protein LOC111023135 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1BSZ19.2e-2459.74uncharacterized protein LOC111005481 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1DX301.5e-1859.42uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6P9EID82.1e-1546.25uncharacterized protein LOC118348856 OS=Juglans regia OX=51240 GN=LOC118348856 P... [more]
K3ZNG23.5e-1548.05CCHC-type domain-containing protein OS=Setaria italica OX=4555 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G01050.11.2e-0432.39zinc ion binding;nucleic acid binding [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 30..78
e-value: 2.8E-16
score: 58.9
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 64..77
score: 9.224166
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 61..79

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS012219.1MS012219.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding