MS012213 (gene) Bitter gourd (TR) v1

BLAST of MS012213 vs. NCBI nr
Match: XP_022148781.1 (triacylglycerol lipase 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 548.5 bits (1412), Expect = 3.5e-152
Identity = 271/306 (88.56%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS EQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD
Sbjct: 60  GDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 119

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL
Sbjct: 120 LAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 179

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGSR 180
           VRLMVDLHLDT+ L      LN +S      L+ L     +         GKNCYLNGSR
Sbjct: 180 VRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSR 239

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
           FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL
Sbjct: 240 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 299

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 300
           PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF
Sbjct: 300 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 359

BLAST of MS012213 vs. NCBI nr
Match: XP_022148780.1 (triacylglycerol lipase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 548.5 bits (1412), Expect = 3.5e-152
Identity = 271/306 (88.56%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS EQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD
Sbjct: 97  GDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 156

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL
Sbjct: 157 LAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 216

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGSR 180
           VRLMVDLHLDT+ L      LN +S      L+ L     +         GKNCYLNGSR
Sbjct: 217 VRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSR 276

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
           FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL
Sbjct: 277 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 336

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 300
           PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF
Sbjct: 337 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 396

BLAST of MS012213 vs. NCBI nr
Match: XP_022148777.1 (triacylglycerol lipase 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 528.9 bits (1361), Expect = 2.9e-146
Identity = 260/305 (85.25%), Postives = 270/305 (88.52%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS +QSLGFILA+NGFDVWIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYD
Sbjct: 97  GDAWFLNSADQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYD 156

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPL
Sbjct: 157 LAEMIDYINSLTDRKVYVVGHSQGTIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPL 216

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGSR 180
           VRLMVDLHLDT+ LA     LN +S      L+ L     N         GKNCYLNGSR
Sbjct: 217 VRLMVDLHLDTMILAMGFHELNFKSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGSR 276

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
           FD FFE+EPHPTSAKNL HLFQMIRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL
Sbjct: 277 FDIFFEYEPHPTSAKNLRHLFQMIRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 336

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 299
           PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 
Sbjct: 337 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFI 396

BLAST of MS012213 vs. NCBI nr
Match: XP_022148778.1 (triacylglycerol lipase 1-like isoform X3 [Momordica charantia])

HSP 1 Score: 524.2 bits (1349), Expect = 7.2e-145
Identity = 260/306 (84.97%), Postives = 270/306 (88.24%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS +QSLGFILA+NGFDVWIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYD
Sbjct: 60  GDAWFLNSADQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYD 119

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPL
Sbjct: 120 LAEMIDYINSLTDRKVYVVGHSQGTIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPL 179

Query: 121 VRLMVDLHLDT-VFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGS 180
           VRLMVDLHLDT + LA     LN +S      L+ L     N         GKNCYLNGS
Sbjct: 180 VRLMVDLHLDTQMILAMGFHELNFKSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGS 239

Query: 181 RFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 240
           RFD FFE+EPHPTSAKNL HLFQMIRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKS
Sbjct: 240 RFDIFFEYEPHPTSAKNLRHLFQMIRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 299

Query: 241 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 299
           LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF
Sbjct: 300 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 359

BLAST of MS012213 vs. NCBI nr
Match: XP_022148776.1 (triacylglycerol lipase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 524.2 bits (1349), Expect = 7.2e-145
Identity = 260/306 (84.97%), Postives = 270/306 (88.24%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS +QSLGFILA+NGFDVWIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYD
Sbjct: 97  GDAWFLNSADQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYD 156

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPL
Sbjct: 157 LAEMIDYINSLTDRKVYVVGHSQGTIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPL 216

Query: 121 VRLMVDLHLDT-VFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGS 180
           VRLMVDLHLDT + LA     LN +S      L+ L     N         GKNCYLNGS
Sbjct: 217 VRLMVDLHLDTQMILAMGFHELNFKSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGS 276

Query: 181 RFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 240
           RFD FFE+EPHPTSAKNL HLFQMIRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKS
Sbjct: 277 RFDIFFEYEPHPTSAKNLRHLFQMIRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 336

Query: 241 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 299
           LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF
Sbjct: 337 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 396

BLAST of MS012213 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 6.8e-106
Identity = 178/306 (58.17%), Postives = 236/306 (77.12%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GD WFLNS ++SLGFILA++GFDVW+GNVRGTR+S+GH++LS+ DK+FW+WSW+DLA+YD
Sbjct: 85  GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 144

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMI Y+ S+++ K+++VGHSQGTI+SF ALTQP +A+ VEAAALL PISYLDH+TAPL
Sbjct: 145 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 204

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFL-------TRHSYNAAGKNCYLNGSR 180
           V  MV +HLD + +A  L  +N +S  +++ ++ L       T    +  G NC  N S+
Sbjct: 205 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 264

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
            +++ ++EPHP+S KN+ HLFQMIRKGTF++YDYG   NLR YG SKPP F L  IP SL
Sbjct: 265 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 324

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 300
           P+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   AKEDVY  MI+FF
Sbjct: 325 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFF 384

BLAST of MS012213 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.2e-56
Identity = 121/313 (38.66%), Postives = 182/313 (58.15%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           G +W LN  +Q+L  ILA+ GFDVW+GN RGTR+S  H  L+   + FWNW+W++L  YD
Sbjct: 109 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 168

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L  M D+I+ LT +K++ +GHS GT+I F + ++  +  +V +AA+LSP++YL H+T   
Sbjct: 169 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTT-- 228

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNL--NFLTRHSYNA-----------AGKNC 180
             ++ D+   T FLA   SIL          L  +F+      A            GKNC
Sbjct: 229 --VIGDIAAKT-FLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNC 288

Query: 181 YLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFDL 240
            LN S  D F   EP  TS KN+ HL Q +R     +Y+YG  + N++ YG++ PPA+++
Sbjct: 289 CLNASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNI 348

Query: 241 RRIPKSLPLWMAYGGNDELSDWTDL-----QHTIKELDSVPELVYLENYGHVDFILSVRA 295
             IP  LPL+ +YGG D L+D  D+     Q    ++D +  + ++++Y H DFI+ V A
Sbjct: 349 SAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKM-NVQFVKDYAHADFIMGVTA 408

BLAST of MS012213 vs. ExPASy Swiss-Prot
Match: O61866 (Lipase lipl-5 OS=Caenorhabditis elegans OX=6239 GN=lipl-5 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.7e-36
Identity = 100/314 (31.85%), Postives = 158/314 (50.32%), Query Frame = 0

Query: 4   WFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLGE 63
           W +N  +QS GF+ A+ GFDVW+GN+RG  +S  H  L      FW+WSW+++A YDL  
Sbjct: 88  WVVNLPDQSAGFLFADAGFDVWLGNMRGNTYSMKHKDLKPSHSAFWDWSWDEMATYDLNA 147

Query: 64  MIDYINSLTDR-KVYVVGHSQGTIISFVALTQPD--IAKKVEAAALLSPISYLDHIT--- 123
           MI+++  +T +  VY +GHSQGT+  F  L++ D   AKK++    L+PI  + HI    
Sbjct: 148 MINHVLEVTGQDSVYYMGHSQGTLTMFSHLSKDDGSFAKKIKKFFALAPIGSVKHIKGFL 207

Query: 124 ---APLVRLMVDLHLDTVFLACYL--------------SILNVQSIQILRNLNFLTRHSY 183
              A    L  D   D      +L                L V++  +  N+ FL     
Sbjct: 208 SFFANYFSLEFDGWFDIFGAGEFLPNNWAMKLAAKDICGGLKVEA-DLCDNVLFLI---- 267

Query: 184 NAAGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSK 243
            A  ++   N +R   +   +P  TS +N+ H  QM+  G    YD+G   N + YG++ 
Sbjct: 268 -AGPESDQWNQTRVPVYATHDPAGTSTQNIVHWMQMVHHGGVPAYDWGTKTNKKKYGQAN 327

Query: 244 PPAFDLRRIPKSLPLWMAYGGNDELSDWTDL-QHTIKELDS--VPELVYLENYGHVDFIL 292
           PP +D   I K   +++ +   D L+D  D+  + +  L+   V +  +L +Y H+DF  
Sbjct: 328 PPEYDFTAI-KGTKIYLYWSDADWLADTPDVPDYLLTRLNPAIVAQNNHLPDYNHLDFTW 387

BLAST of MS012213 vs. ExPASy Swiss-Prot
Match: Q4R4S5 (Lysosomal acid lipase/cholesteryl ester hydrolase OS=Macaca fascicularis OX=9541 GN=LIPA PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.7e-36
Identity = 102/309 (33.01%), Postives = 163/309 (52.75%), Query Frame = 0

Query: 4   WFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLGE 63
           W  N    SLGFILA+ GFDVW+GN RG  WS  H +LS    +FW +S++++A YDL  
Sbjct: 95  WVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPA 154

Query: 64  MIDYI-NSLTDRKVYVVGHSQGTIISFVALTQ-PDIAKKVEAAALLSPISYLDHITAPLV 123
            I++I N     +VY VGHSQGT I F+A +Q P++AK+++    L+P+  +D  T+P+ 
Sbjct: 155 SINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALAPVVSVDFCTSPMA 214

Query: 124 R-------LMVDLHLDTVFL--ACYLSIL------NVQSIQILRNLNFLTRHSYNAAGKN 183
           +       L+ DL  D  FL  + +L  L      +V   ++  NL FL    +N     
Sbjct: 215 KLGRLPDLLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLL-CGFNERN-- 274

Query: 184 CYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYG-LLNNLRVYGRSKPPAFD 243
             LN SR D +    P  TS +N+ H  Q ++   F  +D+G    N   Y +S PP ++
Sbjct: 275 --LNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYN 334

Query: 244 LRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDV 295
           ++ +     +W   GG+D L+D  D+   + ++ ++     +  + H+DFI  + A   +
Sbjct: 335 VKDMLVPTAVWS--GGHDWLADVYDINILLTQITNLVFHESIPEWEHLDFIWGLDAPWRL 394

BLAST of MS012213 vs. ExPASy Swiss-Prot
Match: Q64194 (Lysosomal acid lipase/cholesteryl ester hydrolase OS=Rattus norvegicus OX=10116 GN=Lipa PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-35
Identity = 107/312 (34.29%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 4   WFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLGE 63
           W  N    SLGFILA+ GFDVW+GN RG  WS  H +LS    ++W +S++++A YDL  
Sbjct: 93  WVTNIDNNSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEYWAFSFDEMAKYDLPA 152

Query: 64  MIDYI-NSLTDRKVYVVGHSQGTIISFVALTQ-PDIAKKVEAAALLSPISYLDHITAPLV 123
            I+YI N     ++Y VGHSQG  I F+A +Q P++AKKV+    L+P+  L+  + P+V
Sbjct: 153 SINYILNKTGQEQLYNVGHSQGCTIGFIAFSQMPELAKKVKMFFALAPVLSLNFASGPMV 212

Query: 124 RL--MVDLHLDTVF----------LACYLS---ILNVQSIQILRNLNFLTRHSYNAAGKN 183
           +L  + DL L+ +F          +  +LS     +V   ++  N+ FL    +N   KN
Sbjct: 213 KLGRLPDLLLEDLFGQKQFLPQSAMVKWLSTHICTHVIMKELCANIFFLI-CGFNE--KN 272

Query: 184 CYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFD 243
             LN SR D +    P  TS +N+ H  Q+++      +D+G  + N   Y +S PP + 
Sbjct: 273 --LNMSRVDVYTTHCPAGTSVQNMVHWTQVVKYHKLQAFDWGSSDKNYFHYNQSYPPLYS 332

Query: 244 LRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLEN---YGHVDFILSVRAK 295
           ++ +     LW   GG D L+D +D+   + E   +P LVY +N   + H+DFI  + A 
Sbjct: 333 IKDMQLPTALWS--GGKDWLADTSDINILLTE---IPTLVYHKNIPEWDHLDFIWGLDAP 392

BLAST of MS012213 vs. ExPASy TrEMBL
Match: A0A6J1D3W4 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017353 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.7e-152
Identity = 271/306 (88.56%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS EQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD
Sbjct: 97  GDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 156

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL
Sbjct: 157 LAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 216

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGSR 180
           VRLMVDLHLDT+ L      LN +S      L+ L     +         GKNCYLNGSR
Sbjct: 217 VRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSR 276

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
           FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL
Sbjct: 277 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 336

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 300
           PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF
Sbjct: 337 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 396

BLAST of MS012213 vs. ExPASy TrEMBL
Match: A0A6J1D6F2 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017353 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.7e-152
Identity = 271/306 (88.56%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS EQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD
Sbjct: 60  GDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 119

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL
Sbjct: 120 LAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 179

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGSR 180
           VRLMVDLHLDT+ L      LN +S      L+ L     +         GKNCYLNGSR
Sbjct: 180 VRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSR 239

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
           FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL
Sbjct: 240 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 299

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 300
           PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF
Sbjct: 300 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 359

BLAST of MS012213 vs. ExPASy TrEMBL
Match: A0A6J1D5Z8 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.4e-146
Identity = 260/305 (85.25%), Postives = 270/305 (88.52%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS +QSLGFILA+NGFDVWIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYD
Sbjct: 97  GDAWFLNSADQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYD 156

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPL
Sbjct: 157 LAEMIDYINSLTDRKVYVVGHSQGTIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPL 216

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGSR 180
           VRLMVDLHLDT+ LA     LN +S      L+ L     N         GKNCYLNGSR
Sbjct: 217 VRLMVDLHLDTMILAMGFHELNFKSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGSR 276

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
           FD FFE+EPHPTSAKNL HLFQMIRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL
Sbjct: 277 FDIFFEYEPHPTSAKNLRHLFQMIRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 336

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 299
           PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 
Sbjct: 337 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFI 396

BLAST of MS012213 vs. ExPASy TrEMBL
Match: A0A6J1D523 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.5e-145
Identity = 260/306 (84.97%), Postives = 270/306 (88.24%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS +QSLGFILA+NGFDVWIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYD
Sbjct: 60  GDAWFLNSADQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYD 119

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPL
Sbjct: 120 LAEMIDYINSLTDRKVYVVGHSQGTIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPL 179

Query: 121 VRLMVDLHLDT-VFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGS 180
           VRLMVDLHLDT + LA     LN +S      L+ L     N         GKNCYLNGS
Sbjct: 180 VRLMVDLHLDTQMILAMGFHELNFKSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGS 239

Query: 181 RFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 240
           RFD FFE+EPHPTSAKNL HLFQMIRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKS
Sbjct: 240 RFDIFFEYEPHPTSAKNLRHLFQMIRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 299

Query: 241 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 299
           LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF
Sbjct: 300 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 359

BLAST of MS012213 vs. ExPASy TrEMBL
Match: A0A6J1D6E7 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.5e-145
Identity = 260/306 (84.97%), Postives = 270/306 (88.24%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GDAWFLNS +QSLGFILA+NGFDVWIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYD
Sbjct: 97  GDAWFLNSADQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYD 156

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMIDYINSLTDRKVYVVGHSQGTI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPL
Sbjct: 157 LAEMIDYINSLTDRKVYVVGHSQGTIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPL 216

Query: 121 VRLMVDLHLDT-VFLACYLSILNVQSIQILRNLNFLTRHSYNA-------AGKNCYLNGS 180
           VRLMVDLHLDT + LA     LN +S      L+ L     N         GKNCYLNGS
Sbjct: 217 VRLMVDLHLDTQMILAMGFHELNFKSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGS 276

Query: 181 RFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 240
           RFD FFE+EPHPTSAKNL HLFQMIRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKS
Sbjct: 277 RFDIFFEYEPHPTSAKNLRHLFQMIRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKS 336

Query: 241 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 299
           LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF
Sbjct: 337 LPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF 396

BLAST of MS012213 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 385.2 bits (988), Expect = 4.8e-107
Identity = 178/306 (58.17%), Postives = 236/306 (77.12%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           GD WFLNS ++SLGFILA++GFDVW+GNVRGTR+S+GH++LS+ DK+FW+WSW+DLA+YD
Sbjct: 85  GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 144

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L EMI Y+ S+++ K+++VGHSQGTI+SF ALTQP +A+ VEAAALL PISYLDH+TAPL
Sbjct: 145 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 204

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNLNFL-------TRHSYNAAGKNCYLNGSR 180
           V  MV +HLD + +A  L  +N +S  +++ ++ L       T    +  G NC  N S+
Sbjct: 205 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 264

Query: 181 FDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSL 240
            +++ ++EPHP+S KN+ HLFQMIRKGTF++YDYG   NLR YG SKPP F L  IP SL
Sbjct: 265 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 324

Query: 241 PLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFF 300
           P+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   AKEDVY  MI+FF
Sbjct: 325 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFF 384

BLAST of MS012213 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 220.7 bits (561), Expect = 1.6e-57
Identity = 121/313 (38.66%), Postives = 182/313 (58.15%), Query Frame = 0

Query: 1   GDAWFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYD 60
           G +W LN  +Q+L  ILA+ GFDVW+GN RGTR+S  H  L+   + FWNW+W++L  YD
Sbjct: 109 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 168

Query: 61  LGEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPL 120
           L  M D+I+ LT +K++ +GHS GT+I F + ++  +  +V +AA+LSP++YL H+T   
Sbjct: 169 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTT-- 228

Query: 121 VRLMVDLHLDTVFLACYLSILNVQSIQILRNL--NFLTRHSYNA-----------AGKNC 180
             ++ D+   T FLA   SIL          L  +F+      A            GKNC
Sbjct: 229 --VIGDIAAKT-FLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNC 288

Query: 181 YLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFDL 240
            LN S  D F   EP  TS KN+ HL Q +R     +Y+YG  + N++ YG++ PPA+++
Sbjct: 289 CLNASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNI 348

Query: 241 RRIPKSLPLWMAYGGNDELSDWTDL-----QHTIKELDSVPELVYLENYGHVDFILSVRA 295
             IP  LPL+ +YGG D L+D  D+     Q    ++D +  + ++++Y H DFI+ V A
Sbjct: 349 SAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKM-NVQFVKDYAHADFIMGVTA 408

BLAST of MS012213 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 8.8e-08
Identity = 78/322 (24.22%), Postives = 127/322 (39.44%), Query Frame = 0

Query: 4   WFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLGE 63
           W  N V  S  F   + G+DV++GN RG   S  H++ +   K+FW +S  +    D+  
Sbjct: 347 WVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPA 406

Query: 64  MIDYINSLTDR-------------------KVYVVGHSQGTIISFVALTQPDIAKK---V 123
           MI+ I+ +                      K+  + HS G     + +    I +K   +
Sbjct: 407 MIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRL 466

Query: 124 EAAALLSPISYLD--------------HITAPLVRLMVDLHLDTVFLACYLSIL--NVQS 183
               LLSP  + +               I+  L R++   ++ T F    L+ L  +  +
Sbjct: 467 SRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHN 526

Query: 184 IQILRNLNFLTRHSYNAAGKNCYLNGSRFDFFFEFEPHP-TSAKNLHHLFQMIRKGTFSR 243
              L  L   T  SY   G +    G      +     P  S +   HL Q+   G F  
Sbjct: 527 YPALGGL-VQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRM 586

Query: 244 YDYGLLN-NLRVYGRSKPPAFDLRRIPK--SLPLWMAYGGNDEL--SDWTDLQHTIKELD 281
           YDYG  + N+ VYG  +P   DL    K   +P+ +  G ND++  S      + +   D
Sbjct: 587 YDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMR-D 646

BLAST of MS012213 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 8.8e-08
Identity = 78/322 (24.22%), Postives = 127/322 (39.44%), Query Frame = 0

Query: 4   WFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLGE 63
           W  N V  S  F   + G+DV++GN RG   S  H++ +   K+FW +S  +    D+  
Sbjct: 278 WVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPA 337

Query: 64  MIDYINSLTDR-------------------KVYVVGHSQGTIISFVALTQPDIAKK---V 123
           MI+ I+ +                      K+  + HS G     + +    I +K   +
Sbjct: 338 MIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEKPHRL 397

Query: 124 EAAALLSPISYLD--------------HITAPLVRLMVDLHLDTVFLACYLSIL--NVQS 183
               LLSP  + +               I+  L R++   ++ T F    L+ L  +  +
Sbjct: 398 SRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLARDFHN 457

Query: 184 IQILRNLNFLTRHSYNAAGKNCYLNGSRFDFFFEFEPHP-TSAKNLHHLFQMIRKGTFSR 243
              L  L   T  SY   G +    G      +     P  S +   HL Q+   G F  
Sbjct: 458 YPALGGL-VQTLMSYVVGGDSSNWVGVLGLPHYNMNDMPAVSFRVAQHLAQIKHTGKFRM 517

Query: 244 YDYGLLN-NLRVYGRSKPPAFDLRRIPK--SLPLWMAYGGNDEL--SDWTDLQHTIKELD 281
           YDYG  + N+ VYG  +P   DL    K   +P+ +  G ND++  S      + +   D
Sbjct: 518 YDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRNDKVIRSSMVKKHYNVMR-D 577

BLAST of MS012213 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 7.4e-07
Identity = 78/324 (24.07%), Postives = 133/324 (41.05%), Query Frame = 0

Query: 4   WFLNSVEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLGE 63
           W  N V  S  F   + G+DV++GN RG   S  H+  +   K FW +S  + A  D+  
Sbjct: 345 WVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATEDIPA 404

Query: 64  MIDYINSL--TDRKVY-----------------VVGHSQGTIISFVALTQPDIAKK---V 123
           MI+ I+ +  ++ K+Y                 VV HS G     + +    I +K   +
Sbjct: 405 MIEKIHEIKTSELKLYQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKPHRL 464

Query: 124 EAAALLSPISYLDHITAPLVRLMVDLHLDTVFLACYLS------ILNVQSIQILRNLNFL 183
               LLSP  +  H  + +   +++     +FL   LS       +  +  ++L N    
Sbjct: 465 SRLILLSPAGF--HYDSNMCFTLMEYTF--LFLGPVLSRIVPAFYIPTKFFRMLLNKLAR 524

Query: 184 TRHSYNAAG------KNCYLNGSRFDF-------FFEFEPHP-TSAKNLHHLFQMIRKGT 243
             H+Y A G       +  + G   ++        +     P  S +   HL Q+   G 
Sbjct: 525 DFHNYPAVGGLVQTLMSYVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIKHSGK 584

Query: 244 FSRYDYGLLN-NLRVYGRSKPPAFDLRRIPK--SLPLWMAYGGNDELSDWTDLQHTIKEL 281
           F  +DYG  + N+ VYG  +P   DL        +P+ +  G  D++   + ++   + +
Sbjct: 585 FKMFDYGSSSANMDVYGSPEP--LDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYRVM 644