MS012205 (gene) Bitter gourd (TR) v1

BLAST of MS012205 vs. NCBI nr
Match: XP_022148718.1 (GDSL esterase/lipase At2g40250-like [Momordica charantia])

HSP 1 Score: 723.0 bits (1865), Expect = 1.3e-204
Identity = 360/367 (98.09%), Postives = 363/367 (98.91%), Query Frame = 0

Query: 1   MPHRNAAVFALFCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIR 60
           MPHRNAAVFALFC  IIPLPIIS PAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIR
Sbjct: 1   MPHRNAAVFALFCCFIIPLPIISRPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIR 60

Query: 61  ADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGS 120
           ADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGS
Sbjct: 61  ADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGS 120

Query: 121 AGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLL 180
           AGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLF+ISAGSNDLL
Sbjct: 121 AGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFLISAGSNDLL 180

Query: 181 YNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMES 240
           YNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMES
Sbjct: 181 YNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMES 240

Query: 241 LIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLAN 300
           LIP+TDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLAN
Sbjct: 241 LIPNTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLAN 300

Query: 301 PSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYAA 360
           PSKYGFEEKSRGCCGSGLVEMGPMC+AVTPTCTDASKFIFWDAVHPSQAANSILAN YAA
Sbjct: 301 PSKYGFEEKSRGCCGSGLVEMGPMCSAVTPTCTDASKFIFWDAVHPSQAANSILANRYAA 360

Query: 361 ITLSHLA 368
           ITLSHLA
Sbjct: 361 ITLSHLA 367

BLAST of MS012205 vs. NCBI nr
Match: XP_038887146.1 (GDSL esterase/lipase At2g40250-like [Benincasa hispida])

HSP 1 Score: 506.5 bits (1303), Expect = 1.9e-139
Identity = 259/374 (69.25%), Postives = 303/374 (81.02%), Query Frame = 0

Query: 1   MPHRNAAVFALFCSLIIPLPIISLPAASP-PAPAPHIP------AIFAFGDSTVDPGNNN 60
           M HRNA V AL  +++  +P    PA +P P+P+P  P      AIFAFGDSTVDPGNNN
Sbjct: 1   MSHRNAVVLALSITILFQIP---FPATAPWPSPSPSSPPSSQIRAIFAFGDSTVDPGNNN 60

Query: 61  HIVTLIRADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELL 120
           HI TL+RADHLPYGRALP+H ASGRFSDGKL TDY++S LG+ DLLPAYLD +L+D +LL
Sbjct: 61  HIATLLRADHLPYGRALPDHVASGRFSDGKLTTDYISSTLGLYDLLPAYLDSSLTDIDLL 120

Query: 121 GGASFGSAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVIS 180
           GG SFGSAG+GLDE+TAN SN LD+ SQL+YF+EA  RI+K+ GERKG E+VK GLFVIS
Sbjct: 121 GGVSFGSAGTGLDEVTANLSNVLDLESQLNYFEEAMVRIKKMVGERKGEEMVKKGLFVIS 180

Query: 181 AGSNDLLYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLP 240
            G+NDLLYN+Y+LP RMLQFSVS Y  FLL  L SVVQRLY KGAR+++VVGVPP+GCLP
Sbjct: 181 VGTNDLLYNLYMLPTRMLQFSVSGYHDFLLQKLRSVVQRLYKKGARKMVVVGVPPIGCLP 240

Query: 241 LQVTMESLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGP 300
           LQVTM SLIPS   HM+ R CN +QN+ CQAYN  LKAL+  LQA+LVGAKV YLD+YGP
Sbjct: 241 LQVTMGSLIPS--DHMMQRLCNKKQNNDCQAYNKKLKALTFKLQASLVGAKVVYLDVYGP 300

Query: 301 LMDMLANPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSI 360
           +MDM+ANPSKYGFEE S+GCCGSGL+EMGPMCN ++P C DASKFIFWDAVH +QAA SI
Sbjct: 301 IMDMVANPSKYGFEETSKGCCGSGLMEMGPMCNFLSPICYDASKFIFWDAVHLTQAAYSI 360

Query: 361 LANHYAAITLSHLA 368
           LAN  AA  L H +
Sbjct: 361 LANQSAATLLPHFS 369

BLAST of MS012205 vs. NCBI nr
Match: XP_030931282.1 (GDSL esterase/lipase At2g40250 [Quercus lobata])

HSP 1 Score: 419.1 bits (1076), Expect = 4.0e-113
Identity = 209/367 (56.95%), Postives = 268/367 (73.02%), Query Frame = 0

Query: 1   MPHRNAAVFALF-CSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLI 60
           M  R A  F +F  S ++ LPII      P    P+I A+FAFGDST+D GNNNHI T+ 
Sbjct: 1   MNSRKAIFFTIFLISFLVSLPII------PTTATPNITAVFAFGDSTIDSGNNNHITTIF 60

Query: 61  RADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFG 120
           R DH PYG+ LP+H +SGRFS+GKLATDY+ ++LG+  LLPAYLDP+L+D +LL G SFG
Sbjct: 61  RGDHQPYGQDLPDHVSSGRFSNGKLATDYIVASLGLKQLLPAYLDPSLTDNDLLTGVSFG 120

Query: 121 SAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDL 180
           SAG+GLD  T   S+ALD+ +QL YF+EA  RI K  G  K  +IVKN LF+IS GSND+
Sbjct: 121 SAGTGLDPETVALSHALDMQTQLKYFEEALQRIEKKVGAEKARKIVKNALFLISVGSNDM 180

Query: 181 LYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTME 240
           ++N Y +P R L++++  Y + LL  LESV++ LY  GARRI+V G+PP+GCLP+QVT+ 
Sbjct: 181 MFNFYDIPVRALEYTIDNYQNLLLQKLESVIKSLYGAGARRIVVAGLPPIGCLPMQVTLG 240

Query: 241 SLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLA 300
           S+IPS   HML R C N+QN H QAYNN L+ L+S LQA    AKV Y+DIY P+MDM+ 
Sbjct: 241 SIIPS--FHMLQRVCVNQQNFHSQAYNNKLQTLTSRLQAQHHRAKVLYVDIYNPMMDMIM 300

Query: 301 NPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYA 360
           NP KYGFE+  + CCG+GLVEMGP+CNA+   CTDASK++FWDAVHP+QAA  ++AN Y+
Sbjct: 301 NPHKYGFEQTLQACCGTGLVEMGPLCNAIDLKCTDASKYVFWDAVHPTQAAYRVIANLYS 359

Query: 361 AITLSHL 367
              L HL
Sbjct: 361 QNVLPHL 359

BLAST of MS012205 vs. NCBI nr
Match: KAF3976787.1 (hypothetical protein CMV_000042 [Castanea mollissima])

HSP 1 Score: 409.8 bits (1052), Expect = 2.4e-110
Identity = 207/367 (56.40%), Postives = 262/367 (71.39%), Query Frame = 0

Query: 1   MPHRNAAVFAL-FCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLI 60
           M  R A  F +   S ++ LPII      P    P+I  +FAFGDST+D GNNNHI T+ 
Sbjct: 1   MDSRKAIFFTISLISFLVSLPII------PTTATPNITGVFAFGDSTIDSGNNNHITTIF 60

Query: 61  RADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFG 120
           R DH PYG+ LP H +SGRFS+GKLATDY+  +LG+  LLPAYLDP+L+D +LL G SFG
Sbjct: 61  RGDHQPYGQDLPGHVSSGRFSNGKLATDYIVVSLGLKQLLPAYLDPSLTDNDLLTGVSFG 120

Query: 121 SAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDL 180
           SAG+GLD  T   S+ALD+ +QL+YF EA  RI K  G  K   IVKN LF+IS GSND+
Sbjct: 121 SAGTGLDPETVALSHALDMQTQLNYFHEALQRIEKKVGAEKARRIVKNALFLISVGSNDM 180

Query: 181 LYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTME 240
           ++N Y +P R L++++  Y + LL  LESV++ LY  GARRI+V G+PP+GCLP+QVT+ 
Sbjct: 181 MFNFYDIPVRALEYTIDNYHNLLLQKLESVIKSLYGAGARRIVVAGLPPIGCLPMQVTLG 240

Query: 241 SLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLA 300
           S+IPS   HML R C NRQN   QAYNN L+ L+S LQA    AKV Y+DIY P+MDM+ 
Sbjct: 241 SIIPS--FHMLQRVCVNRQNFDSQAYNNKLQTLTSRLQAQHHRAKVLYVDIYNPMMDMIM 300

Query: 301 NPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYA 360
           NP KYGFE+  + CCG+GLVEMGP+CNA+   CTDASK++FWDAVHP+QAA  ++AN Y+
Sbjct: 301 NPHKYGFEQTLQACCGTGLVEMGPLCNAIDLKCTDASKYVFWDAVHPTQAAYRVIANLYS 359

Query: 361 AITLSHL 367
              L HL
Sbjct: 361 QKVLPHL 359

BLAST of MS012205 vs. NCBI nr
Match: XP_023903713.1 (GDSL esterase/lipase At2g40250 [Quercus suber])

HSP 1 Score: 407.1 bits (1045), Expect = 1.6e-109
Identity = 206/367 (56.13%), Postives = 263/367 (71.66%), Query Frame = 0

Query: 1   MPHRNAAVFALF-CSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLI 60
           M  R A  F +F  S ++ LPII      P    P+I A+FAFGDST+D GNNNHI T+ 
Sbjct: 1   MDSRKAIFFTIFLISFLVSLPII------PTTATPNITAVFAFGDSTIDSGNNNHITTIF 60

Query: 61  RADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFG 120
           R DH PYG+ LP H +SGRFS+GKLATDY+ ++LG+  LLPAYLDP+L+D +LL G SFG
Sbjct: 61  RGDHQPYGQDLPGHVSSGRFSNGKLATDYIVASLGLKQLLPAYLDPSLTDNDLLTGVSFG 120

Query: 121 SAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDL 180
           SAG+GLD  T   S+ALD+ +QL YF+EA  R+ K  G  K  +IVKN LF IS GSND+
Sbjct: 121 SAGTGLDPETVALSHALDMQTQLKYFEEALQRMAKKIGAEKARKIVKNALFFISVGSNDM 180

Query: 181 LYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTME 240
           ++N Y +P R L++++  Y + LL  LESV++ LY  GARRI+V G+PP+GCLP+QVT+ 
Sbjct: 181 MFNFYDIPVRALEYTIDNYQNLLLQKLESVIKSLYGAGARRIVVAGLPPIGCLPMQVTLG 240

Query: 241 SLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLA 300
           S+IPS   HML R C N QN   QAYNN L+ L+S LQA    AKV Y+DIY P+MDM+ 
Sbjct: 241 SIIPS--FHMLQRVCVNHQNFDSQAYNNKLQNLTSRLQAQHHRAKVLYVDIYNPMMDMIM 300

Query: 301 NPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYA 360
           NP  YGFE+  + CCG+GLVEMGP+CNA+   CTDASK++FWDAVHP+QAA  ++AN Y+
Sbjct: 301 NPHIYGFEQTLQACCGTGLVEMGPLCNAIDLKCTDASKYLFWDAVHPTQAAYRVIANLYS 359

Query: 361 AITLSHL 367
              L HL
Sbjct: 361 QNVLPHL 359

BLAST of MS012205 vs. ExPASy Swiss-Prot
Match: Q9SIZ6 (GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 7.9e-88
Identity = 167/354 (47.18%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 3   HRNAAVFALFCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIRAD 62
           +++  +F  F   I+ L +++L  AS   P+P I A++AFGDSTVD GNNN+I TL +++
Sbjct: 4   NQHKPMFVTFLINILLLQLLNLTNAS---PSPPITALYAFGDSTVDSGNNNYIPTLFQSN 63

Query: 63  HLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAG 122
           H PYG++ P   ++GRFSDGKLATD++ S+LG+   LPAYL+P++   +LL G SF SAG
Sbjct: 64  HPPYGKSFPSKLSTGRFSDGKLATDFIVSSLGLKPTLPAYLNPSVKPVDLLTGVSFASAG 123

Query: 123 SGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYN 182
            GLD+ TA  S  + +  Q  YF+EA  +++ + G+ +   ++KN +FVISAG+ND+++N
Sbjct: 124 GGLDDRTAKSSLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGTNDMIFN 183

Query: 183 IYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLI 242
           +Y      L  SVS Y   LL  +E  VQRLY  GARRI + G+PP+GCLP+QVT+ S+ 
Sbjct: 184 VYDHVLGSL-ISVSDYQDSLLTKVEVFVQRLYEAGARRITIAGLPPIGCLPVQVTLTSI- 243

Query: 243 PSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPS 302
            +T     HR C   QN   + YN  L+ L   L     G+KV YLDIY PL+DM+ +P 
Sbjct: 244 -NTPRIFHHRICTEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPLIDMIKHPR 303

Query: 303 KYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           KYG EE  RGCCG+GL+E GP+C  ++ TC D SK++F+D+VHPSQ A S++A+
Sbjct: 304 KYGLEETLRGCCGTGLLEAGPLCQPLSRTCDDVSKYLFFDSVHPSQTAYSVIAS 351

BLAST of MS012205 vs. ExPASy Swiss-Prot
Match: Q9SJA9 (GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2 SV=2)

HSP 1 Score: 295.4 bits (755), Expect = 8.7e-79
Identity = 154/345 (44.64%), Postives = 219/345 (63.48%), Query Frame = 0

Query: 14  SLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNN-HIVTLIRADHLPYGRALPE 73
           +L I   + S  AA+     P  PAI  FGDSTVD GNNN H  T+ +A HLPYG  LP 
Sbjct: 10  TLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGIDLPN 69

Query: 74  HAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANF 133
           H ASGRF++GK+ +D +A+ L I   +P +L P LSD E++ G  F SAG+G D+ T+  
Sbjct: 70  HKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHTSLS 129

Query: 134 SNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQ 193
           + A+ +  Q   F    AR++ + G++K  EI+KN L VISAG ND + N Y +P R L+
Sbjct: 130 TQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSRRLE 189

Query: 194 F-SVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLH 253
           F  +S Y  F+L  L++ V+ LYS G R+IMV G+PPMGCLP+Q+T +            
Sbjct: 190 FPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAK-------FRNAL 249

Query: 254 RHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSR 313
           R C  ++N     YN  L+ L   ++A+L G+K+ Y ++Y P+MDM+ NPSKYGF+E  R
Sbjct: 250 RFCLEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKR 309

Query: 314 GCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           GCCG+G +E   MCNA +PTC + S+F+F+D++HPS+A  + + N
Sbjct: 310 GCCGTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATYNYMGN 347

BLAST of MS012205 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-76
Identity = 140/319 (43.89%), Postives = 200/319 (62.70%), Query Frame = 0

Query: 37  PAIFAFGDSTVDPGNNNHIVTLIRADHLPYGRALPEHAASGRFSDGKLATDYLASALGIN 96
           PAI  FGDST+D GNNN+I T IRA+  PYG   P H A+GRFS+GKL  D++AS +GI 
Sbjct: 36  PAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIK 95

Query: 97  DLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVA 156
           D +P +LDP LSD +++ G  F SAGSG D LT   ++ L +  Q D       R+ ++ 
Sbjct: 96  DTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQIV 155

Query: 157 GERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSK 216
           G+ K   IV   L ++S+G+ND   N+Y  P R  +  V  Y  F+L N+ + VQ LY  
Sbjct: 156 GDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDI 215

Query: 217 GARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNL 276
           G R+IMV+G+PP+GCLP+Q+TM             R C ++QN   Q +N  LK   + +
Sbjct: 216 GCRKIMVLGLPPVGCLPIQMTMAM------QKQNERRCIDKQNSDSQEFNQKLKNSLTEM 275

Query: 277 QANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDAS 336
           Q+NL G+ + Y DIYG L DM  NP +YG +E +RGCCG+G +E+  +CNA+T  C + +
Sbjct: 276 QSNLTGSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPN 335

Query: 337 KFIFWDAVHPSQAANSILA 356
           +++FWD +HPSQ A  +++
Sbjct: 336 QYLFWDDIHPSQIAYIVIS 348

BLAST of MS012205 vs. ExPASy Swiss-Prot
Match: Q9SIQ3 (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.4e-76
Identity = 143/333 (42.94%), Postives = 211/333 (63.36%), Query Frame = 0

Query: 26  AASPPAPAPHIPAIFAFGDSTVDPGNNNH-IVTLIRADHLPYGRALPEHAASGRFSDGKL 85
           AA+     P  PAI  FGDSTVD GNNN+ + T+ RA+H PYG  LP+  A+GRFS+GKL
Sbjct: 23  AAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKL 82

Query: 86  ATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANFSNALDIWSQLDY 145
            +D +A+ L I + +P +L P LSD ++L G  F SAG+G D+LT+  + A+ +  Q + 
Sbjct: 83  ISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNM 142

Query: 146 FDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQFS-VSQYLHFLL 205
           F    AR++ + G++K  EI+ N   V+SAG ND + N Y +P R L++  +S Y  F+L
Sbjct: 143 FKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFIL 202

Query: 206 HNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLHRHCNNRQNHHCQ 265
             LE+ V+ LYS G R ++V G+PPMGCLP+ +T +          + R C    N    
Sbjct: 203 KRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAK-------FRNIFRFCLEHHNKDSV 262

Query: 266 AYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSRGCCGSGLVEMGP 325
            YN  L+ L   ++A+L G+K  Y D+Y P+M+M+ NPSKYGF+E  RGCCG+G +E   
Sbjct: 263 LYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSF 322

Query: 326 MCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           MCN  +P C + S+F+F+D++HPS+A  +++ N
Sbjct: 323 MCNVFSPVCQNRSEFLFFDSIHPSEATYNVIGN 348

BLAST of MS012205 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 288.1 bits (736), Expect = 1.4e-76
Identity = 144/338 (42.60%), Postives = 213/338 (63.02%), Query Frame = 0

Query: 21  IISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNH-IVTLIRADHLPYGRALPEHAASGRF 80
           + S  AA+     P  PAI  FGDSTVD GNNN+ + T+ RA+H PYG  LP+  A+GRF
Sbjct: 18  LASCDAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRF 77

Query: 81  SDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANFSNALDIW 140
           S+GKL +D +A+ L I + +P +L P LSD ++L G  F SAG+G D+LT+  + A+ + 
Sbjct: 78  SNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVS 137

Query: 141 SQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQFS-VSQY 200
            Q + F    AR++ + G++K  EI+ N   V+SAG ND + N Y +P R L++  +S Y
Sbjct: 138 EQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYDIPSRRLEYPFISGY 197

Query: 201 LHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLHRHCNNRQ 260
             F+L  LE+ V+ LYS G R ++V G+PPMGCLP+ +T +          + R C    
Sbjct: 198 QDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAK-------FRNIFRFCLEHH 257

Query: 261 NHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSRGCCGSGL 320
           N     YN  L+ L   ++A+L G+K  Y D+Y P+M+M+ NPSKYGF+E  RGCCG+G 
Sbjct: 258 NKDSVLYNEKLQKLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGF 317

Query: 321 VEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           +E   MCN  +P C + S+F+F+D++HPS+A  +++ N
Sbjct: 318 LETSFMCNVFSPVCQNRSEFMFFDSIHPSEATYNVIGN 348

BLAST of MS012205 vs. ExPASy TrEMBL
Match: A0A6J1D4W0 (GDSL esterase/lipase At2g40250-like OS=Momordica charantia OX=3673 GN=LOC111017313 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 6.2e-205
Identity = 360/367 (98.09%), Postives = 363/367 (98.91%), Query Frame = 0

Query: 1   MPHRNAAVFALFCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIR 60
           MPHRNAAVFALFC  IIPLPIIS PAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIR
Sbjct: 1   MPHRNAAVFALFCCFIIPLPIISRPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIR 60

Query: 61  ADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGS 120
           ADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGS
Sbjct: 61  ADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGS 120

Query: 121 AGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLL 180
           AGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLF+ISAGSNDLL
Sbjct: 121 AGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFLISAGSNDLL 180

Query: 181 YNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMES 240
           YNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMES
Sbjct: 181 YNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMES 240

Query: 241 LIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLAN 300
           LIP+TDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLAN
Sbjct: 241 LIPNTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLAN 300

Query: 301 PSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYAA 360
           PSKYGFEEKSRGCCGSGLVEMGPMC+AVTPTCTDASKFIFWDAVHPSQAANSILAN YAA
Sbjct: 301 PSKYGFEEKSRGCCGSGLVEMGPMCSAVTPTCTDASKFIFWDAVHPSQAANSILANRYAA 360

Query: 361 ITLSHLA 368
           ITLSHLA
Sbjct: 361 ITLSHLA 367

BLAST of MS012205 vs. ExPASy TrEMBL
Match: A0A7N2MGH0 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.9e-113
Identity = 209/367 (56.95%), Postives = 268/367 (73.02%), Query Frame = 0

Query: 1   MPHRNAAVFALF-CSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLI 60
           M  R A  F +F  S ++ LPII      P    P+I A+FAFGDST+D GNNNHI T+ 
Sbjct: 1   MNSRKAIFFTIFLISFLVSLPII------PTTATPNITAVFAFGDSTIDSGNNNHITTIF 60

Query: 61  RADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFG 120
           R DH PYG+ LP+H +SGRFS+GKLATDY+ ++LG+  LLPAYLDP+L+D +LL G SFG
Sbjct: 61  RGDHQPYGQDLPDHVSSGRFSNGKLATDYIVASLGLKQLLPAYLDPSLTDNDLLTGVSFG 120

Query: 121 SAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDL 180
           SAG+GLD  T   S+ALD+ +QL YF+EA  RI K  G  K  +IVKN LF+IS GSND+
Sbjct: 121 SAGTGLDPETVALSHALDMQTQLKYFEEALQRIEKKVGAEKARKIVKNALFLISVGSNDM 180

Query: 181 LYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTME 240
           ++N Y +P R L++++  Y + LL  LESV++ LY  GARRI+V G+PP+GCLP+QVT+ 
Sbjct: 181 MFNFYDIPVRALEYTIDNYQNLLLQKLESVIKSLYGAGARRIVVAGLPPIGCLPMQVTLG 240

Query: 241 SLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLA 300
           S+IPS   HML R C N+QN H QAYNN L+ L+S LQA    AKV Y+DIY P+MDM+ 
Sbjct: 241 SIIPS--FHMLQRVCVNQQNFHSQAYNNKLQTLTSRLQAQHHRAKVLYVDIYNPMMDMIM 300

Query: 301 NPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYA 360
           NP KYGFE+  + CCG+GLVEMGP+CNA+   CTDASK++FWDAVHP+QAA  ++AN Y+
Sbjct: 301 NPHKYGFEQTLQACCGTGLVEMGPLCNAIDLKCTDASKYVFWDAVHPTQAAYRVIANLYS 359

Query: 361 AITLSHL 367
              L HL
Sbjct: 361 QNVLPHL 359

BLAST of MS012205 vs. ExPASy TrEMBL
Match: A0A2G5D7W8 (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02600214v1 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.8e-103
Identity = 203/369 (55.01%), Postives = 262/369 (71.00%), Query Frame = 0

Query: 4   RNAAVFALFCSLIIPLPIISLPAAS----PPAPAPHIPAIFAFGDSTVDPGNNNHIVT-L 63
           ++A  F LF + I+   ++ L A++       P+  I A+FAFGDST+DPGNN+HI T L
Sbjct: 2   KSAIFFFLFLASIVSHHVVPLHASAAVNDSKIPSYGISAVFAFGDSTLDPGNNDHIGTIL 61

Query: 64  IRADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASF 123
            R DHLPYGR LP HAASGRFS+GKL  D++ S LG+ +LLPAYLD  ++D ELL G SF
Sbjct: 62  FRGDHLPYGRDLPHHAASGRFSNGKLMLDFVVSNLGLKNLLPAYLDRGVNDRELLTGVSF 121

Query: 124 GSAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSND 183
            SAG+GLD +TA  SN L + +QLDYF +A  RIRK  G     +IV N LF I  G+ND
Sbjct: 122 ASAGTGLDAVTAAISNVLPMSAQLDYFQQALGRIRKAVGSETADQIVNNALFTIGVGTND 181

Query: 184 LLYNIYLLPKRMLQF-SVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVT 243
           L++N Y LP R  QF +VS+Y  FLL  LE+VVQRLY  GAR+  + G+PP+GCLP+QVT
Sbjct: 182 LMWNFYDLPTRRGQFITVSRYHDFLLGRLEAVVQRLYRMGARKFSISGLPPIGCLPIQVT 241

Query: 244 MESLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDM 303
             SL+PST  HML R C  +QN   Q+YN  L++L S LQA L G++VAY+DIY PL+DM
Sbjct: 242 AGSLVPST--HMLQRICVEQQNLDSQSYNAKLQSLISRLQARLPGSRVAYVDIYNPLVDM 301

Query: 304 LANPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANH 363
           + NP KYGF+E +RGCCG+G+ EMG +CN + PTC DASKF+FWDAVHP+QA   +LA+ 
Sbjct: 302 IHNPHKYGFQEATRGCCGTGIFEMGAVCNGLVPTCPDASKFVFWDAVHPTQATYQVLADS 361

Query: 364 YAAITLSHL 367
           ++A  +  L
Sbjct: 362 FSANVVPRL 368

BLAST of MS012205 vs. ExPASy TrEMBL
Match: A0A7J6VY45 (GDSL esterase/lipase OS=Thalictrum thalictroides OX=46969 GN=FRX31_020374 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 3.1e-103
Identity = 199/369 (53.93%), Postives = 257/369 (69.65%), Query Frame = 0

Query: 3   HRNAAVFALFCSL---IIPLPIISLPAASPPAPAPH-IPAIFAFGDSTVDPGNNNHIVTL 62
           +++   F L C L   ++PL   +        PA H I A+FAFGDST+DPGNN+HI T+
Sbjct: 5   YKSVIFFFLACFLCHHVLPLGASASVNEDSKIPAQHGISAVFAFGDSTLDPGNNDHIATV 64

Query: 63  IRADHLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASF 122
            R DHLPYGR LP H ASGRF++GKL  D++   LG+ DLLPAYLD  ++D ELL G SF
Sbjct: 65  FRGDHLPYGRDLPRHQASGRFTNGKLMLDFVVKNLGLKDLLPAYLDRRVTDRELLTGVSF 124

Query: 123 GSAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSND 182
            SAG+GLD+LTA  SN L + +QLD+F +A  RI +  G     EIV N LF I  G+ND
Sbjct: 125 ASAGTGLDDLTATISNVLPMSAQLDHFQQALGRIHRAVGTEIADEIVNNALFTIGVGTND 184

Query: 183 LLYNIYLLPKRMLQF-SVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVT 242
           L++N Y LP R  +F +VS Y  FLL  LESVVQRLY  GAR+  + G+PP+GCLP+QVT
Sbjct: 185 LIWNFYDLPTRRGEFLTVSSYHDFLLGRLESVVQRLYRMGARKFSISGLPPIGCLPVQVT 244

Query: 243 MESLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDM 302
             S++PST  H+L R C  +QN   Q+YN  L+ L S LQA L G+KVAY+DIY PL+DM
Sbjct: 245 AGSILPST--HLLQRVCVGQQNFDSQSYNAKLQGLISRLQARLPGSKVAYVDIYNPLVDM 304

Query: 303 LANPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANH 362
           + NP KYGF+E +RGCCG+G++EMG +CN++ PTC DASKF+FWDAVHP+QA   +LA+ 
Sbjct: 305 IHNPHKYGFQETTRGCCGTGMLEMGSLCNSLVPTCPDASKFVFWDAVHPTQATYQVLAHS 364

Query: 363 YAAITLSHL 367
           + A  +  L
Sbjct: 365 FMANVVPRL 371

BLAST of MS012205 vs. ExPASy TrEMBL
Match: A0A1U7ZSA1 (GDSL esterase/lipase At2g40250-like OS=Nelumbo nucifera OX=4432 GN=LOC104597364 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 3.1e-103
Identity = 192/362 (53.04%), Postives = 246/362 (67.96%), Query Frame = 0

Query: 5   NAAVFALFCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIRADHL 64
           ++A F++F         + +P  + P+    I A++AFGDST+DPGNNNH+ TL R DH 
Sbjct: 7   SSAAFSIFLIFSASFLFLVVPLNAAPS---KISAVYAFGDSTLDPGNNNHLGTLFRGDHP 66

Query: 65  PYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSG 124
           PYGR  P   A+GRF+DG+L TD+  S+LGI DLLPAYLDP +S+ +LL G SF SAG+G
Sbjct: 67  PYGRDFPGQIATGRFTDGRLMTDFTVSSLGIKDLLPAYLDPRVSNLDLLTGVSFASAGTG 126

Query: 125 LDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIY 184
           +D+LTA  SN   + +QL YF++  AR++   G      IV++ LF I  G+ND+++N Y
Sbjct: 127 IDDLTATISNVAHMSTQLHYFEQCLARMQSTVGREAASRIVEDALFAIGIGTNDMIFNFY 186

Query: 185 LLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPS 244
            LP R  QFS+  Y  FLLHNLES V RLYS GARR  + G+PP+GCLP+Q T  S++P 
Sbjct: 187 DLPTRRGQFSLPAYHDFLLHNLESFVMRLYSMGARRFSISGLPPIGCLPVQETAGSILPI 246

Query: 245 TDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKY 304
              HML R CN+ +N   QAYN  L+AL   LQA L G + AY+DIY PL+DM+ NP KY
Sbjct: 247 PGGHMLQRVCNDAENQDSQAYNAKLQALVLRLQATLPGTRFAYVDIYNPLLDMITNPGKY 306

Query: 305 GFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILANHYAAITLS 364
           GF E  RGCCG+GLVEMG MCNA TP C D SKF+FWDAVHP+Q    ILAN + A  + 
Sbjct: 307 GFVETRRGCCGTGLVEMGGMCNAATPVCPDPSKFVFWDAVHPTQTTYQILANIFTATVIP 365

Query: 365 HL 367
           HL
Sbjct: 367 HL 365

BLAST of MS012205 vs. TAIR 10
Match: AT2G40250.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 5.6e-89
Identity = 167/354 (47.18%), Postives = 238/354 (67.23%), Query Frame = 0

Query: 3   HRNAAVFALFCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNNHIVTLIRAD 62
           +++  +F  F   I+ L +++L  AS   P+P I A++AFGDSTVD GNNN+I TL +++
Sbjct: 4   NQHKPMFVTFLINILLLQLLNLTNAS---PSPPITALYAFGDSTVDSGNNNYIPTLFQSN 63

Query: 63  HLPYGRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAG 122
           H PYG++ P   ++GRFSDGKLATD++ S+LG+   LPAYL+P++   +LL G SF SAG
Sbjct: 64  HPPYGKSFPSKLSTGRFSDGKLATDFIVSSLGLKPTLPAYLNPSVKPVDLLTGVSFASAG 123

Query: 123 SGLDELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYN 182
            GLD+ TA  S  + +  Q  YF+EA  +++ + G+ +   ++KN +FVISAG+ND+++N
Sbjct: 124 GGLDDRTAKSSLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGTNDMIFN 183

Query: 183 IYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLI 242
           +Y      L  SVS Y   LL  +E  VQRLY  GARRI + G+PP+GCLP+QVT+ S+ 
Sbjct: 184 VYDHVLGSL-ISVSDYQDSLLTKVEVFVQRLYEAGARRITIAGLPPIGCLPVQVTLTSI- 243

Query: 243 PSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPS 302
            +T     HR C   QN   + YN  L+ L   L     G+KV YLDIY PL+DM+ +P 
Sbjct: 244 -NTPRIFHHRICTEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPLIDMIKHPR 303

Query: 303 KYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           KYG EE  RGCCG+GL+E GP+C  ++ TC D SK++F+D+VHPSQ A S++A+
Sbjct: 304 KYGLEETLRGCCGTGLLEAGPLCQPLSRTCDDVSKYLFFDSVHPSQTAYSVIAS 351

BLAST of MS012205 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 295.4 bits (755), Expect = 6.2e-80
Identity = 154/345 (44.64%), Postives = 219/345 (63.48%), Query Frame = 0

Query: 14  SLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNN-HIVTLIRADHLPYGRALPE 73
           +L I   + S  AA+     P  PAI  FGDSTVD GNNN H  T+ +A HLPYG  LP 
Sbjct: 10  TLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGIDLPN 69

Query: 74  HAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANF 133
           H ASGRF++GK+ +D +A+ L I   +P +L P LSD E++ G  F SAG+G D+ T+  
Sbjct: 70  HKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHTSLS 129

Query: 134 SNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQ 193
           + A+ +  Q   F    AR++ + G++K  EI+KN L VISAG ND + N Y +P R L+
Sbjct: 130 TQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSRRLE 189

Query: 194 F-SVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLH 253
           F  +S Y  F+L  L++ V+ LYS G R+IMV G+PPMGCLP+Q+T +            
Sbjct: 190 FPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAK-------FRNAL 249

Query: 254 RHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSR 313
           R C  ++N     YN  L+ L   ++A+L G+K+ Y ++Y P+MDM+ NPSKYGF+E  R
Sbjct: 250 RFCLEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKR 309

Query: 314 GCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           GCCG+G +E   MCNA +PTC + S+F+F+D++HPS+A  + + N
Sbjct: 310 GCCGTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATYNYMGN 347

BLAST of MS012205 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 7.6e-78
Identity = 140/319 (43.89%), Postives = 200/319 (62.70%), Query Frame = 0

Query: 37  PAIFAFGDSTVDPGNNNHIVTLIRADHLPYGRALPEHAASGRFSDGKLATDYLASALGIN 96
           PAI  FGDST+D GNNN+I T IRA+  PYG   P H A+GRFS+GKL  D++AS +GI 
Sbjct: 36  PAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIK 95

Query: 97  DLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANFSNALDIWSQLDYFDEATARIRKVA 156
           D +P +LDP LSD +++ G  F SAGSG D LT   ++ L +  Q D       R+ ++ 
Sbjct: 96  DTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQIV 155

Query: 157 GERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQFSVSQYLHFLLHNLESVVQRLYSK 216
           G+ K   IV   L ++S+G+ND   N+Y  P R  +  V  Y  F+L N+ + VQ LY  
Sbjct: 156 GDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDI 215

Query: 217 GARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLHRHCNNRQNHHCQAYNNGLKALSSNL 276
           G R+IMV+G+PP+GCLP+Q+TM             R C ++QN   Q +N  LK   + +
Sbjct: 216 GCRKIMVLGLPPVGCLPIQMTMAM------QKQNERRCIDKQNSDSQEFNQKLKNSLTEM 275

Query: 277 QANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSRGCCGSGLVEMGPMCNAVTPTCTDAS 336
           Q+NL G+ + Y DIYG L DM  NP +YG +E +RGCCG+G +E+  +CNA+T  C + +
Sbjct: 276 QSNLTGSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPN 335

Query: 337 KFIFWDAVHPSQAANSILA 356
           +++FWD +HPSQ A  +++
Sbjct: 336 QYLFWDDIHPSQIAYIVIS 348

BLAST of MS012205 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.9e-78
Identity = 143/333 (42.94%), Postives = 211/333 (63.36%), Query Frame = 0

Query: 26  AASPPAPAPHIPAIFAFGDSTVDPGNNNH-IVTLIRADHLPYGRALPEHAASGRFSDGKL 85
           AA+     P  PAI  FGDSTVD GNNN+ + T+ RA+H PYG  LP+  A+GRFS+GKL
Sbjct: 23  AAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKL 82

Query: 86  ATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSGLDELTANFSNALDIWSQLDY 145
            +D +A+ L I + +P +L P LSD ++L G  F SAG+G D+LT+  + A+ +  Q + 
Sbjct: 83  ISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNM 142

Query: 146 FDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIYLLPKRMLQFS-VSQYLHFLL 205
           F    AR++ + G++K  EI+ N   V+SAG ND + N Y +P R L++  +S Y  F+L
Sbjct: 143 FKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFIL 202

Query: 206 HNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPSTDHHMLHRHCNNRQNHHCQ 265
             LE+ V+ LYS G R ++V G+PPMGCLP+ +T +          + R C    N    
Sbjct: 203 KRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAK-------FRNIFRFCLEHHNKDSV 262

Query: 266 AYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKYGFEEKSRGCCGSGLVEMGP 325
            YN  L+ L   ++A+L G+K  Y D+Y P+M+M+ NPSKYGF+E  RGCCG+G +E   
Sbjct: 263 LYNEKLQNLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSF 322

Query: 326 MCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           MCN  +P C + S+F+F+D++HPS+A  +++ N
Sbjct: 323 MCNVFSPVCQNRSEFLFFDSIHPSEATYNVIGN 348

BLAST of MS012205 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.1e-76
Identity = 149/351 (42.45%), Postives = 216/351 (61.54%), Query Frame = 0

Query: 8   VFALFCSLIIPLPIISLPAASPPAPAPHIPAIFAFGDSTVDPGNNN-HIVTLIRADHLPY 67
           VF LF + +    ++S   A+     P  PAI  FGDSTVD GNNN H  T+ +A HLPY
Sbjct: 8   VFGLFVATL----LVSCNVAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPY 67

Query: 68  GRALPEHAASGRFSDGKLATDYLASALGINDLLPAYLDPTLSDGELLGGASFGSAGSGLD 127
           G  LP H A+GR+S+GK+ +D +AS L I +L+P +L P +S  +++ G SF SAG+G D
Sbjct: 68  GVDLPGHEANGRYSNGKVISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYD 127

Query: 128 ELTANFSNALDIWSQLDYFDEATARIRKVAGERKGGEIVKNGLFVISAGSNDLLYNIYLL 187
           + ++  S A+ +  Q   F    AR++ + G++K  EI+ N L VISAG ND + N Y +
Sbjct: 128 DRSSLSSKAIPVSQQPSMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDI 187

Query: 188 PKRMLQF-SVSQYLHFLLHNLESVVQRLYSKGARRIMVVGVPPMGCLPLQVTMESLIPST 247
           P R L++ ++  Y  F+L  L+  V+ LYS G R I+V G+PPMGCLP+Q+T +      
Sbjct: 188 PTRRLEYPTIHGYQEFILKRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKM----- 247

Query: 248 DHHMLHRHCNNRQNHHCQAYNNGLKALSSNLQANLVGAKVAYLDIYGPLMDMLANPSKYG 307
               + R C  ++N     YN  L      +QA+L G+   Y ++Y PLMDM+ NPSKYG
Sbjct: 248 --RNILRFCVEQENKDSVLYNQKLVKKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKYG 307

Query: 308 FEEKSRGCCGSGLVEMGPMCNAVTPTCTDASKFIFWDAVHPSQAANSILAN 357
           F+E  +GCCG+G +E   MCN +T TC + S  +FWD++HPS+AA + + N
Sbjct: 308 FKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHLFWDSIHPSEAAYNYIGN 347