MS012048 (gene) Bitter gourd (TR) v1

Overview


Name	MS012048
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Werner Syndrome-like exonuclease
Location	scaffold54: 143364 .. 143981 (+)
RNA-Seq Expression	MS012048
Synteny	MS012048

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCACCAGCGCCAGCCACCGAATCCCCTCCGATTCCGACAATTTATTCGACGTCACCATAGATTCCGATCATATTCGCACTCTCCTCACATCTTCTCCGGCCATGGTCGATTCCTGGATCTCTGAAACCCTCGCGATTCGGACTCCACCTCTCATCGTTGGTTTGTACATCGAGTGGCGCCTCAACGCCAATCGCTCTCACAGAGACCGGGTCACCACATTGGAACTCTGTTTCAATCGCCGATGCCTCATTTTCCAACTGATTCACGCCTCCGAAATCCCTCGCTCTCTGTTCGATTTCTTGAACAACGAGTCGTTCAGTTTCGTCGGAGTTGGAATCGGCAGGCACGTCGAGAAGCTCTACAGCGATCACGGCTTGAGAGTGAGAAATGCTGTGGATCTCATGAGTTTGGTCGAGAATGTGATGGGGAGAAGAGATCTGAGATGTGCGGGATTGAGGGGCTTGGCGAAAGAGATTCTTGGAAAGATGATTTCTGAGCCGAAGGACGCGACGACGTGTAGGTGGGATAATGAATGGCTGACTATGAATCAGGTGTCGTATGCTTCTGTTGATGTGTTTCTGTCATCTGAGATTGGAAGACGCCTTTCGATT

mRNA sequence

ATGGCTACCACCAGCGCCAGCCACCGAATCCCCTCCGATTCCGACAATTTATTCGACGTCACCATAGATTCCGATCATATTCGCACTCTCCTCACATCTTCTCCGGCCATGGTCGATTCCTGGATCTCTGAAACCCTCGCGATTCGGACTCCACCTCTCATCGTTGGTTTGTACATCGAGTGGCGCCTCAACGCCAATCGCTCTCACAGAGACCGGGTCACCACATTGGAACTCTGTTTCAATCGCCGATGCCTCATTTTCCAACTGATTCACGCCTCCGAAATCCCTCGCTCTCTGTTCGATTTCTTGAACAACGAGTCGTTCAGTTTCGTCGGAGTTGGAATCGGCAGGCACGTCGAGAAGCTCTACAGCGATCACGGCTTGAGAGTGAGAAATGCTGTGGATCTCATGAGTTTGGTCGAGAATGTGATGGGGAGAAGAGATCTGAGATGTGCGGGATTGAGGGGCTTGGCGAAAGAGATTCTTGGAAAGATGATTTCTGAGCCGAAGGACGCGACGACGTGTAGGTGGGATAATGAATGGCTGACTATGAATCAGGTGTCGTATGCTTCTGTTGATGTGTTTCTGTCATCTGAGATTGGAAGACGCCTTTCGATT

Coding sequence (CDS)

ATGGCTACCACCAGCGCCAGCCACCGAATCCCCTCCGATTCCGACAATTTATTCGACGTCACCATAGATTCCGATCATATTCGCACTCTCCTCACATCTTCTCCGGCCATGGTCGATTCCTGGATCTCTGAAACCCTCGCGATTCGGACTCCACCTCTCATCGTTGGTTTGTACATCGAGTGGCGCCTCAACGCCAATCGCTCTCACAGAGACCGGGTCACCACATTGGAACTCTGTTTCAATCGCCGATGCCTCATTTTCCAACTGATTCACGCCTCCGAAATCCCTCGCTCTCTGTTCGATTTCTTGAACAACGAGTCGTTCAGTTTCGTCGGAGTTGGAATCGGCAGGCACGTCGAGAAGCTCTACAGCGATCACGGCTTGAGAGTGAGAAATGCTGTGGATCTCATGAGTTTGGTCGAGAATGTGATGGGGAGAAGAGATCTGAGATGTGCGGGATTGAGGGGCTTGGCGAAAGAGATTCTTGGAAAGATGATTTCTGAGCCGAAGGACGCGACGACGTGTAGGTGGGATAATGAATGGCTGACTATGAATCAGGTGTCGTATGCTTCTGTTGATGTGTTTCTGTCATCTGAGATTGGAAGACGCCTTTCGATT

Protein sequence

MATTSASHRIPSDSDNLFDVTIDSDHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIEWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNEWLTMNQVSYASVDVFLSSEIGRRLSI

Homology

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BLAST of MS012048 vs. NCBI nr
Match: XP_022147750.1 (Werner Syndrome-like exonuclease [Momordica charantia])

HSP 1 Score: 298.5 bits (763), Expect = 4.4e-77
Identity = 150/205 (73.17%), Postives = 165/205 (80.49%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIE 60
           MA +   H IPSDS NL+DVTIDSD I TLLTSSPAMVDSWISETLAI+TPPLIVGL IE
Sbjct: 1   MAISIVDHEIPSDSHNLYDVTIDSDQIHTLLTSSPAMVDSWISETLAIQTPPLIVGLDIE 60

Query: 61  WRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVE 120
           WR N N S+ + V TL+LCF+RRCLIFQLIHASEIPRSL DFL +ESF+FVGVGIG  VE
Sbjct: 61  WRPNRNSSYENPVATLQLCFDRRCLIFQLIHASEIPRSLTDFLTDESFTFVGVGIGEDVE 120

Query: 121 KLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNE 180
           KL  D+GL V N VDL +L EN MGR DL+ AGL+GLAKE+LGK IS+PK  T  RWDNE
Sbjct: 121 KLLCDYGLNVSNTVDLRNLAENAMGRGDLKNAGLKGLAKEVLGKEISKPKSVTMSRWDNE 180

Query: 181 WLTMNQVSYASVDVFLSSEIGRRLS 206
           WL  NQV YA VD FLSSEIGR LS
Sbjct: 181 WLVPNQVKYACVDAFLSSEIGRHLS 205

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BLAST of MS012048 vs. NCBI nr
Match: XP_011652174.1 (Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical protein Csa_001395 [Cucumis sativus])

HSP 1 Score: 248.4 bits (633), Expect = 5.2e-62
Identity = 124/205 (60.49%), Postives = 151/205 (73.66%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TLAI+TPPL+VGL I
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT+NQV YA +D F S EIG+ L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGKFL 204

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BLAST of MS012048 vs. NCBI nr
Match: XP_008443244.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exonuclease [Cucumis melo subsp. melo] >KAA0053896.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK25510.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 246.9 bits (629), Expect = 1.5e-61
Identity = 123/205 (60.00%), Postives = 150/205 (73.17%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

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BLAST of MS012048 vs. NCBI nr
Match: KAG6580879.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017635.1 Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 236.1 bits (601), Expect = 2.7e-58
Identity = 125/205 (60.98%), Postives = 145/205 (70.73%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T      PS S N FDVT +SD  I TLLT SP MVD WISETL  +TPPLIVGL I
Sbjct: 1   MALTIVDREDPSGSHNYFDVTFESDEPILTLLTISPLMVDDWISETLNTQTPPLIVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N  RS+ + V TL+LC  RRCLI QLI+A E P+SL++FL NES SFVGVGI    
Sbjct: 61  EWRPNI-RSYENPVATLQLCTGRRCLIVQLIYAPEFPKSLYEFLENESISFVGVGIEGDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+VR  VDL +L E+V GRR+L+ AGL+ LAKEILGK I +P+  T  RWD 
Sbjct: 121 EKLNCDYGLKVRKTVDLRNLAESVTGRRELKNAGLKSLAKEILGKEIRKPRGVTLSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT NQ+ YA  D F+S EIGR L
Sbjct: 181 EWLTHNQIKYACFDAFVSYEIGRHL 204

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BLAST of MS012048 vs. NCBI nr
Match: XP_038903327.1 (Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida])

HSP 1 Score: 235.0 bits (598), Expect = 6.0e-58
Identity = 117/188 (62.23%), Postives = 144/188 (76.60%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T   H++PSDS N FD+T+DSD  I TLLT+SP+MV++WISETLA++TPPL+VGL I
Sbjct: 1   MAITIVDHQVPSDSHNYFDITLDSDEPILTLLTNSPSMVENWISETLALQTPPLMVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QLIH  EIP+SLF+FL NES +FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCMGRRCLILQLIHTPEIPKSLFEFLENESLTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   VDL +L E+V GRRDL+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRVDLRNLAESVTGRRDLKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQV 188
           EWLT+NQ+
Sbjct: 181 EWLTLNQM 187

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BLAST of MS012048 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.8e-10
Identity = 42/155 (27.10%), Postives = 74/155 (47.74%), Query Frame = 0


Query: 54  IVGLYIEWRLNANRSHRDRVTTLELCFN-RRCLIFQLIHASEIPRSLFDFLNNESFSFVG 113
           ++G  IEW     +    +V  +++C + ++C +F +   +  P+ L   L +ES   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 114 VGIGRHVEKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCA---GLRGLAKEILGKMISEP 173
           VGI     KL SD+ L+++  ++L     + M  + LRC       GL K +  + + + 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIEL-----SEMANQKLRCKEKWTFNGLIKHLFKEQLYKR 191

Query: 174 KDATTCRWDNEWLTMNQVSYASVDVFLSSEIGRRL 205
           K      WD   LT +Q  YA+ D +    I ++L
Sbjct: 192 KSYRCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

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BLAST of MS012048 vs. ExPASy Swiss-Prot
Match: Q5UQM4 (Putative 3'-5' exonuclease R431 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_R431 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.5e-07
Identity = 47/191 (24.61%), Postives = 88/191 (46.07%), Query Frame = 0


Query: 25  DHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIEWRLNANRSHRDRVTTLELCFNRRC 84
           +++  ++TS   +VD+WI   +       I+GL  E  ++      ++++ ++L  ++  
Sbjct: 108 NNLDIIVTSDFQIVDNWIENNI-YDLKQEIIGLDTETLISGK---SEKISIIQLSTSKHN 167

Query: 85  LIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKL--YSDHGLRVRNAVDLMSLVEN 144
           +I Q+   + +P++L     +ES   VGV I    +KL  Y     +++  +DL  L + 
Sbjct: 168 IIIQVNQMNTLPQNLNKVFFDESIIKVGVAIDIDAKKLLQYFPTINQIKKTLDLSDLFKQ 227

Query: 145 VMGRRDL-----RCAGLRGLAKEILGKMISEPKDA--TTCRWDNEWLTMNQVSYASVDVF 204
               + +        GL+ LA  +L   I    D+      W+N  LT +QV YA  D +
Sbjct: 228 TNFTKHISINPKESIGLKILAAHVLDLYIENKGDSEIKKSNWNNPVLTSDQVKYAITDSY 287

Query: 205 LSSEIGRRLSI 207
           LS  I   L +
Sbjct: 288 LSLMIYNELQL 294

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BLAST of MS012048 vs. ExPASy Swiss-Prot
Match: B4JF25 (Werner Syndrome-like exonuclease OS=Drosophila grimshawi OX=7222 GN=WRNexo PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.4e-04
Identity = 43/182 (23.63%), Postives = 77/182 (42.31%), Query Frame = 0


Query: 31  LTSSPAMVDSWISETLAIRTPPLIVGLYIEWRLNANRSHRDRVTTLELCFNRR-CLIFQL 90
           + +S   V  W+ + + +   P+   +  EW  +  ++   + + +++C + R C ++QL
Sbjct: 115 IAASADEVMQWVEKQINMDVVPMAFDM--EWPFSF-QTGPGKSSVIQICVDERCCYVYQL 174

Query: 91  IHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYSDHGLR-----VRNAVDLMSLVENV- 150
              ++IP +L   +N+      GV I     KL  D         +   VDL      V 
Sbjct: 175 SKLNKIPAALAALINHPKVRLHGVNIKADFRKLERDFPEMSAEPLIEKCVDLGVWCNQVC 234

Query: 151 -MGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNEWLTMNQVSYASVDVFLSSEIGR 205
             G R      L  LA  I  K + + K     +W    L  NQ+ YA++DV++   I R
Sbjct: 235 ETGGR----WSLERLANFIAKKAMDKSKKVRMSKWHVIPLDENQLMYAAIDVYIGQVIYR 289

[Close]

BLAST of MS012048 vs. ExPASy Swiss-Prot
Match: B4N9D3 (Werner Syndrome-like exonuclease OS=Drosophila willistoni OX=7260 GN=WRNexo PE=3 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-04
Identity = 44/182 (24.18%), Postives = 75/182 (41.21%), Query Frame = 0


Query: 31  LTSSPAMVDSWISETLAIRTPPLIVGLYIEWRLNANRSHRDRVTTLELCFNRR-CLIFQL 90
           + +S   V  WI +   +   P+   +  EW  +  ++   + + +++C + R C ++QL
Sbjct: 140 IAASADDVIQWIDKQTTLDVVPMAFDM--EWPFSF-QTGPGKSSVIQVCVDERCCYVYQL 199

Query: 91  IHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYSDH-----GLRVRNAVDLMSLVENV- 150
               ++P +L   LN+      GV I     KL  D         +   VDL      + 
Sbjct: 200 SKLKKLPAALVALLNHPKVRLHGVNIKADFRKLQRDFPEVSADPLIEKCVDLGVWCNEIC 259

Query: 151 -MGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNEWLTMNQVSYASVDVFLSSEIGR 205
             G R      L  LA  I  K + + K     +W    L  NQ+ YA++DV++   I R
Sbjct: 260 ETGGR----WSLERLANFIAKKAMDKSKKVRMSKWHVIPLDENQLMYAAIDVYIGQVIYR 314

[Close]

BLAST of MS012048 vs. ExPASy Swiss-Prot
Match: B3LWP6 (Werner Syndrome-like exonuclease OS=Drosophila ananassae OX=7217 GN=WRNexo PE=3 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 2.3e-04
Identity = 37/137 (27.01%), Postives = 63/137 (45.99%), Query Frame = 0


Query: 74  TTLELCFNRR-CLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYSDH-----G 133
           + +++C + + C I+QL +  ++P +L   +N+      GV I     KL  D       
Sbjct: 179 SVIQICVDEKCCYIYQLTNLKKLPSALVALINHPKVRLHGVNIKADFRKLQRDFPEVSAD 238

Query: 134 LRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNEWLTMNQV 193
             +   VDL  +  NV+ +   R + L  LA  I  K + + K     +W    L  NQ+
Sbjct: 239 ALIEKCVDL-GVWCNVICQTGGRWS-LERLANFICRKAMDKSKKVRMSKWHVIPLDENQL 298

Query: 194 SYASVDVFLSSEIGRRL 205
            YA++DV++   I R L
Sbjct: 299 MYAAIDVYIGQVIYRDL 313

[Close]

BLAST of MS012048 vs. ExPASy TrEMBL
Match: A0A6J1D107 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016613 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.1e-77
Identity = 150/205 (73.17%), Postives = 165/205 (80.49%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDHIRTLLTSSPAMVDSWISETLAIRTPPLIVGLYIE 60
           MA +   H IPSDS NL+DVTIDSD I TLLTSSPAMVDSWISETLAI+TPPLIVGL IE
Sbjct: 1   MAISIVDHEIPSDSHNLYDVTIDSDQIHTLLTSSPAMVDSWISETLAIQTPPLIVGLDIE 60

Query: 61  WRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVE 120
           WR N N S+ + V TL+LCF+RRCLIFQLIHASEIPRSL DFL +ESF+FVGVGIG  VE
Sbjct: 61  WRPNRNSSYENPVATLQLCFDRRCLIFQLIHASEIPRSLTDFLTDESFTFVGVGIGEDVE 120

Query: 121 KLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNE 180
           KL  D+GL V N VDL +L EN MGR DL+ AGL+GLAKE+LGK IS+PK  T  RWDNE
Sbjct: 121 KLLCDYGLNVSNTVDLRNLAENAMGRGDLKNAGLKGLAKEVLGKEISKPKSVTMSRWDNE 180

Query: 181 WLTMNQVSYASVDVFLSSEIGRRLS 206
           WL  NQV YA VD FLSSEIGR LS
Sbjct: 181 WLVPNQVKYACVDAFLSSEIGRHLS 205

[Close]

BLAST of MS012048 vs. ExPASy TrEMBL
Match: A0A0A0LBN3 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819870 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 2.5e-62
Identity = 124/205 (60.49%), Postives = 151/205 (73.66%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           MA T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TLAI+TPPL+VGL I
Sbjct: 1   MAITIVDHEVPSDSHNYFDITFDTDEPILTLLTTSPSMVDGWISQTLAIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHMPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT+NQV YA +D F S EIG+ L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGKFL 204

[Close]

BLAST of MS012048 vs. ExPASy TrEMBL
Match: A0A5A7UI20 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006600 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.4e-62
Identity = 123/205 (60.00%), Postives = 150/205 (73.17%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MS012048 vs. ExPASy TrEMBL
Match: E5GBP0 (3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.4e-62
Identity = 123/205 (60.00%), Postives = 150/205 (73.17%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MS012048 vs. ExPASy TrEMBL
Match: A0A1S3B738 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.4e-62
Identity = 123/205 (60.00%), Postives = 150/205 (73.17%), Query Frame = 0


Query: 1   MATTSASHRIPSDSDNLFDVTIDSDH-IRTLLTSSPAMVDSWISETLAIRTPPLIVGLYI 60
           M+ T   H +PSDS N FD+T D+D  I TLLT+SP+MVD WIS+TL I+TPPL+VGL I
Sbjct: 1   MSITIVDHEVPSDSHNYFDITFDNDEPILTLLTTSPSMVDDWISQTLTIQTPPLLVGLDI 60

Query: 61  EWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHV 120
           EWR N NRS+ + V TL+LC  RRCLI QL+H  EIP+SLF+FL NESF+FVGVGI    
Sbjct: 61  EWRPN-NRSYDNPVATLQLCIGRRCLILQLLHIPEIPKSLFEFLENESFTFVGVGIDEDA 120

Query: 121 EKLYSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDN 180
           EKL  D+GL+V   +DL  L E+V GR +L+ AGL+ L KE+LGK I +PK  T  RWD 
Sbjct: 121 EKLNCDYGLKVGKRMDLRDLAESVTGRGELKNAGLKRLGKEVLGKEIHKPKSVTMSRWDQ 180

Query: 181 EWLTMNQVSYASVDVFLSSEIGRRL 205
           EWLT+NQV YA +D F S EIGR L
Sbjct: 181 EWLTLNQVKYACIDAFFSFEIGRFL 204

[Close]

BLAST of MS012048 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.8e-13
Identity = 53/197 (26.90%), Postives = 89/197 (45.18%), Query Frame = 0


Query: 30  LLTSSPAMVDSWISETLA---IRTPPLIVGLYIEWRLNA------------------NRS 89
           ++T  P+++  WI + L+     + PL+VG+ ++W  ++                   RS
Sbjct: 29  IVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRYRSDSPPVRYRS 88

Query: 90  HRDRV--------TTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVE 149
              RV         TL+LC   RC+I QL H   +P+ L +FL +  ++FVG+   +   
Sbjct: 89  DSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDYTFVGIWNSQDAG 148

Query: 150 KL-YSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILGKM-ISEPKDATTCRWD 196
           KL  S H L +   +DL   V +  GR   RC+    + +E LG   +   +  +   W 
Sbjct: 149 KLKRSKHELEIAVLLDLRKFVSDSSGRTMKRCS-FEKIVEENLGHSGVRLDRKVSRSDWR 208

[Close]

BLAST of MS012048 vs. TAIR 10
Match: AT3G12460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 5.3e-12
Identity = 49/209 (23.44%), Postives = 90/209 (43.06%), Query Frame = 0


Query: 18  FDVTIDSDHIRTLLTSSPAMVDSWISETL---AIRTPPLIVGLYIEWRLNANRSHRDRVT 77
           + V    + +   +T  P+++  WI +      + + PL+VG+ ++W  +        V+
Sbjct: 17  YSVDFFGEELFVTVTPDPSVIGQWIHDVFFHNRLSSHPLVVGVGVQWTPSGYHPASPPVS 76

Query: 78  --------------------------TLELCFNRRCLIFQLIHASEIPRSLFDFLNNESF 137
                                     TL+LC   RC+I QL +   +P+ L +FL +   
Sbjct: 77  YRSDSSPDSYRSDSPPVFYSSDPPADTLQLCVGNRCIIIQLRYCERVPQVLRNFLADRDN 136

Query: 138 SFVGVGIGRHVEKL-YSDHGLRVRNAVDLMSLVENVMGRRDLRCAGLRGLAKEILG-KMI 196
           +FVG+   +   KL  S H L +   +DL   V +  GRR +    L  + +E LG   +
Sbjct: 137 TFVGIWNSQDAGKLERSRHQLEIAELMDLREFVSDSSGRRSMYNYSLEKIVEENLGYPGV 196

[Close]

BLAST of MS012048 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 6.9e-12
Identity = 47/153 (30.72%), Postives = 76/153 (49.67%), Query Frame = 0


Query: 52  PLIVGLYIEWRLNANRSHRDRVTTLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFV 111
           PL+VGL ++W    +    D    L+LC   RCLI QL H   IP  L  FL +E+ +FV
Sbjct: 55  PLVVGLDVQWTPGGSDPPPD---ILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFV 114

Query: 112 GVGIGRHVEKLYSDHGLRVRNAVDLMSL--VENVMGRRDLRCAGLRGLAKEILG-KMISE 171
           GV   +   KL      R R+ +++  L  + + +  R L  +    + +E LG K + +
Sbjct: 115 GVWNSQDQGKLE-----RFRHQLEIWRLLDIRHYLPTRLLN-SSFEKIVEECLGYKGVRK 174

Query: 172 PKDATTCRWDNEWLTMNQVSYASVDVFLSSEIG 202
            K+     W    L+ +Q+  AS DV++  ++G
Sbjct: 175 DKEICMSNWGARSLSHDQIVQASDDVYVCCKLG 198

[Close]

BLAST of MS012048 vs. TAIR 10
Match: AT3G12470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 48/187 (25.67%), Postives = 87/187 (46.52%), Query Frame = 0


Query: 25  DHIRTLLTSSPAMVDSWISETLAIR----TPPLIVGLYIEWRLNANRSHRDRVTTLELCF 84
           D +   +T + +++  WI    +        PL+VG+ ++WR +++        TL+LC 
Sbjct: 24  DDLIVTVTPTASVIRRWIRSVRSYNRNHSVHPLVVGIGVQWRPDSS-DPDPPPKTLQLCV 83

Query: 85  NRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKLYS-DHGLRVRNAVDLMSL 144
             RCLI QL +   +P+ L  FL +   +FVGV  G+  +KL    H + +   +D+   
Sbjct: 84  GSRCLIIQLGYNYGLPKVLRTFLADPKTTFVGVWNGQDQKKLEKCRHRVEIGKLLDIRMF 143

Query: 145 VENVMGRRDLRCAGLRGLAKEILGKMISEPKDATTCRWDNEWLTMNQVSYASVDVFLSSE 204
           V +  G R   C+    + KE LG+ +    D   C   ++W   N   Y  +   + S 
Sbjct: 144 VRDSRGARMCFCS-FEQIVKERLGR-VGVRLDPAICM--SDWGVYNLNHYQVLQASIESY 203

Query: 205 IGRRLSI 207
           +  +L++
Sbjct: 204 VCFKLAV 205

[Close]

BLAST of MS012048 vs. TAIR 10
Match: AT3G12440.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 6.5e-10
Identity = 41/131 (31.30%), Postives = 66/131 (50.38%), Query Frame = 0


Query: 75  TLELCFNRRCLIFQLIHASEIPRSLFDFLNNESFSFVGVGIGRHVEKL-YSDHGLRVRNA 134
           TL+LC   RC+I QL + + +P  L  FL + + +FVG    +   KL  S H L +   
Sbjct: 218 TLQLCVGNRCIIIQLFYCNRVPHVLRRFLGDRNHTFVGFWNSQDAGKLKRSRHRLEIAEL 277

Query: 135 VDLMSLVENVMGRRDLRCAGLRGLAKEILG-KMISEPKDATTCRWDNEWLTMNQVSYASV 194
           +DL + V +  G R L+      +  E LG + +   K      W    L+ +Q+  AS+
Sbjct: 278 LDLRNHVVDSRG-RGLKGRSFEVVVGECLGYRGVRLEKAIGMSDWSVYDLSYDQILQASI 337

Query: 195 DVFLSSEIGRR 204
           DV++ S++G R
Sbjct: 338 DVYVCSKLGFR 347

The following BLAST results are available for this feature:

BLAST of MS012048 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147750.1	4.4e-77	73.17	Werner Syndrome-like exonuclease [Momordica charantia]	[more]
XP_011652174.1	5.2e-62	60.49	Werner Syndrome-like exonuclease [Cucumis sativus] >KGN59430.1 hypothetical prot...	[more]
XP_008443244.1	1.5e-61	60.00	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo] >ADN33882.1 3'-5' exo...	[more]
KAG6580879.1	2.7e-58	60.98	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_038903327.1	6.0e-58	62.23	Werner Syndrome-like exonuclease isoform X2 [Benincasa hispida]	[more]

BLAST of MS012048 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O93530	4.8e-10	27.10	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
Q5UQM4	8.5e-07	24.61	Putative 3'-5' exonuclease R431 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN...	[more]
B4JF25	1.4e-04	23.63	Werner Syndrome-like exonuclease OS=Drosophila grimshawi OX=7222 GN=WRNexo PE=3 ...	[more]
B4N9D3	1.8e-04	24.18	Werner Syndrome-like exonuclease OS=Drosophila willistoni OX=7260 GN=WRNexo PE=3...	[more]
B3LWP6	2.3e-04	27.01	Werner Syndrome-like exonuclease OS=Drosophila ananassae OX=7217 GN=WRNexo PE=3 ...	[more]

BLAST of MS012048 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D107	2.1e-77	73.17	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016613 ...	[more]
A0A0A0LBN3	2.5e-62	60.49	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5A7UI20	7.4e-62	60.00	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
E5GBP0	7.4e-62	60.00	3'-5' exonuclease OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A1S3B738	7.4e-62	60.00	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103486879 PE=4 SV...	[more]

BLAST of MS012048 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12430.1	4.8e-13	26.90	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12460.1	5.3e-12	23.44	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	6.9e-12	30.72	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12470.1	1.7e-10	25.67	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12440.1	6.5e-10	31.30	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 40..199 e-value: 1.7E-10 score: 40.9
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 14..205 e-value: 2.3E-45 score: 156.5
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 10..205
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 10..205
None	No IPR available	CDD	cd06141	WRN_exo	coord: 32..204 e-value: 1.13996E-46 score: 149.651
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 46..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS012048.1	MS012048.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding