MS011758 (gene) Bitter gourd (TR) v1

Overview


Name	MS011758
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold11: 446282 .. 448007 (+)
RNA-Seq Expression	MS011758
Synteny	MS011758

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGCAATACTAAAAGCATGGAAAGCAGCATGTAGCTCTAAAACAGAAAGTTCTACAGTGCTAATTCCCAAGAGGCATACACTTCTAGTAAAACCAATTGTTTTGAGAGGTCCATGCAGGGCAAACAGCATCACTTTCATGGTAAAGAAAAAGCAGGGGCTTTCCCCTTTATGATTAAGTCTTCCCTAGATACTACCTGTCTTCAGGTAGATGCAAATTTTCACAGATACATGGAGAGATTGTGGCACCAACCTCACCAGGCGAATGGACGCGGACTGACCAGAGTCAGTGGCTGGCTTTTGAAGGGGTAAGCAGGCTCAATGTCACTGGCACCGGCATTGGCAAAATAGATGGAAGAGGTACAAGATGGTGGAGCCAATCATGTAGGGATCACCCAGACTTGGTAAGTAAATCATCAGATAAGAAAAACTTTCCAATGCATATAAAGTAATATCCAGTGTCTGATGTTTCTTCTACATTTTTCAGAAGGGCTGCACCTCATTAGCACCTACTGTAAGTATTTTCTGCACAAAGCAATTTTCTAGCATCTGCTAGAGAGAGTGAGAGAGTATTTGATGAACTTACTAAATCTCGGGCATTATTGCAGGCAATGAAGATTATTTCATGCAACAACACCAGCTTGAGCAACATTCACTTTATTAACAGCTCACAAACACATGTACTAATAATGGGGAGCAAGAGAGTCGAAATTAAAAATTTGGTAATCACAGCTCCAGAAAGCAGCCCAAACACTGATGGCATTCACATTCATTCCTCTCACAATGTTATTATAAAGAACACAACAATAGGCACAGGTAATTTGGATGGAATTATTTCCAGAAAGATCGGCTTTAACAAGATTAACAAGTATCGACATTTTTAATTAAGAAAATAAATAACATTAATTGTGACGAGCAGGTGATGATTGTGTATCTATAGGAGATTACACCTCTAACGTGAATATCTCACACATCAGATGTGGACCTGGTCATGGAATAAGGTAGGTTCCCTATTCAGATAAGGAACTCCGTAAAACACGCTTTAGAACAAGAACACGGAAAAATATAGAACAAAATTCTTTGTTTCAATAGCTCATTTCGTATACTATGTGAACCTGAAAATAGCATTGGAAGCTTGGGAAGAAGCGGCAATTTTGTACAAGTAGAGGATATTAGAGTGAGTAATGTCTACTTCAAAGGAACTACCAATGGAGCTCGGATTAAGACTTGGCAGGTAATGGAAATTCTACTCATTAATTTATAAAATAAACTGTAAAGCTTACACTAACACTTCCAAAAGATTGGTAGGGGTAACATCCGAAGGGTCACATTTCAGAATCTCTATTTTGAATCAGTCAATAACCCCTTTATTATAGACCAAAACTACGGTAACCTAGGGGGACTCAGCAACGAAAAGGTAAAAGAAACTGAGCTTCTTTACTAGATTAGTTTTAGTTTTTTCATAATTCAGTGCTCAGGAAATTTTGAACTTGCCCCAGCAAACCGGAGTTCATATAGCCAATGTATCCATCAATAACCTTTTTGGGACATCAATCAGCAATGTGGCTATCAATCTCAATTGCAGTAGGTCTGTTGCCTGCACTGGTATACTGTTAAAGTCGATACAGCTCAAATCTGCAAATAGTGGACAAAAGGTCACCTCAAGTTGCCTCAATGCCCATGGATTTTCCTTTGGAGTGGTACAACCTGAACCCTGTCTTCAGATA

mRNA sequence

CAGGCAATACTAAAAGCATGGAAAGCAGCATGTAGCTCTAAAACAGAAAGTTCTACAGTGCTAATTCCCAAGAGGCATACACTTCTAGTAAAACCAATTGTTTTGAGAGGTCCATGCAGGGCAAACAGCATCACTTTCATGATACATGGAGAGATTGTGGCACCAACCTCACCAGGCGAATGGACGCGGACTGACCAGAGTCAGTGGCTGGCTTTTGAAGGGGTAAGCAGGCTCAATGTCACTGGCACCGGCATTGGCAAAATAGATGGAAGAGGTACAAGATGGTGGAGCCAATCATGTAGGGATCACCCAGACTTGGCAATGAAGATTATTTCATGCAACAACACCAGCTTGAGCAACATTCACTTTATTAACAGCTCACAAACACATGTACTAATAATGGGGAGCAAGAGAGTCGAAATTAAAAATTTGGTAATCACAGCTCCAGAAAGCAGCCCAAACACTGATGGCATTCACATTCATTCCTCTCACAATGTTATTATAAAGAACACAACAATAGGCACAGCAGGTGATGATTGTGTATCTATAGGAGATTACACCTCTAACGTGAATATCTCACACATCAGATGTGGACCTGGTCATGGAATAAGCATTGGAAGCTTGGGAAGAAGCGGCAATTTTGTACAAGTAGAGGATATTAGAGTGAGTAATGTCTACTTCAAAGGAACTACCAATGGAGCTCGGATTAAGACTTGGCAGATTGGTAGGGGTAACATCCGAAGGGTCACATTTCAGAATCTCTATTTTGAATCAGTCAATAACCCCTTTATTATAGACCAAAACTACGGTAACCTAGGGGGACTCAGCAACGAAAAGCAAACCGGAGTTCATATAGCCAATGTATCCATCAATAACCTTTTTGGGACATCAATCAGCAATGTGGCTATCAATCTCAATTGCAGTAGGTCTGTTGCCTGCACTGGTATACTGTTAAAGTCGATACAGCTCAAATCTGCAAATAGTGGACAAAAGGTCACCTCAAGTTGCCTCAATGCCCATGGATTTTCCTTTGGAGTGGTACAACCTGAACCCTGTCTTCAGATA

Coding sequence (CDS)

CAGGCAATACTAAAAGCATGGAAAGCAGCATGTAGCTCTAAAACAGAAAGTTCTACAGTGCTAATTCCCAAGAGGCATACACTTCTAGTAAAACCAATTGTTTTGAGAGGTCCATGCAGGGCAAACAGCATCACTTTCATGATACATGGAGAGATTGTGGCACCAACCTCACCAGGCGAATGGACGCGGACTGACCAGAGTCAGTGGCTGGCTTTTGAAGGGGTAAGCAGGCTCAATGTCACTGGCACCGGCATTGGCAAAATAGATGGAAGAGGTACAAGATGGTGGAGCCAATCATGTAGGGATCACCCAGACTTGGCAATGAAGATTATTTCATGCAACAACACCAGCTTGAGCAACATTCACTTTATTAACAGCTCACAAACACATGTACTAATAATGGGGAGCAAGAGAGTCGAAATTAAAAATTTGGTAATCACAGCTCCAGAAAGCAGCCCAAACACTGATGGCATTCACATTCATTCCTCTCACAATGTTATTATAAAGAACACAACAATAGGCACAGCAGGTGATGATTGTGTATCTATAGGAGATTACACCTCTAACGTGAATATCTCACACATCAGATGTGGACCTGGTCATGGAATAAGCATTGGAAGCTTGGGAAGAAGCGGCAATTTTGTACAAGTAGAGGATATTAGAGTGAGTAATGTCTACTTCAAAGGAACTACCAATGGAGCTCGGATTAAGACTTGGCAGATTGGTAGGGGTAACATCCGAAGGGTCACATTTCAGAATCTCTATTTTGAATCAGTCAATAACCCCTTTATTATAGACCAAAACTACGGTAACCTAGGGGGACTCAGCAACGAAAAGCAAACCGGAGTTCATATAGCCAATGTATCCATCAATAACCTTTTTGGGACATCAATCAGCAATGTGGCTATCAATCTCAATTGCAGTAGGTCTGTTGCCTGCACTGGTATACTGTTAAAGTCGATACAGCTCAAATCTGCAAATAGTGGACAAAAGGTCACCTCAAGTTGCCTCAATGCCCATGGATTTTCCTTTGGAGTGGTACAACCTGAACCCTGTCTTCAGATA

Protein sequence

QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLAMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPCLQI

Homology

[Close]

BLAST of MS011758 vs. NCBI nr
Match: XP_022143115.1 (probable polygalacturonase At1g80170 [Momordica charantia])

HSP 1 Score: 703.0 bits (1813), Expect = 1.3e-198
Identity = 353/363 (97.25%), Postives = 353/363 (97.25%), Query Frame = 0


Query: 2   AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW 61
           AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW
Sbjct: 47  AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW 106

Query: 62  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKIIS 121
           TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL         AMKIIS
Sbjct: 107 TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLKGCTSLAPTAMKIIS 166

Query: 122 CNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTT 181
           CNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTT
Sbjct: 167 CNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTT 226

Query: 182 IGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTN 241
           IGT GDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTN
Sbjct: 227 IGT-GDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTN 286

Query: 242 GARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINN 301
           GARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINN
Sbjct: 287 GARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINN 346

Query: 302 LFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC 356
           LFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC
Sbjct: 347 LFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC 406

[Close]

BLAST of MS011758 vs. NCBI nr
Match: KAA0061603.1 (putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 503.4 bits (1295), Expect = 1.6e-138
Identity = 263/387 (67.96%), Postives = 292/387 (75.45%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFM------------- 60
           QAI KAWKAAC+SKT+SSTVLIPKR T++VKPI+L GPCRA SITFM             
Sbjct: 46  QAISKAWKAACNSKTKSSTVLIPKRQTVMVKPILLSGPCRAKSITFMVKKNRDFPLMIKS 105

Query: 61  ---------------IHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRG 120
                          I+G IVAPTSP EW  TD+S WLAF+G+SRL++TG+GIGKIDGRG
Sbjct: 106 FLEYYLSSGRCKLSQINGVIVAPTSPKEWAGTDKSLWLAFKGISRLSITGSGIGKIDGRG 165

Query: 121 TRWWSQSCRDHPDL----AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITA 180
           T WW+QSCRDHP L    A KIISCN+T+LSN+HFINSSQ HVL+M  +R EIKNLVITA
Sbjct: 166 TGWWNQSCRDHPSLPLLQATKIISCNDTNLSNLHFINSSQAHVLLMECRRTEIKNLVITA 225

Query: 181 PESSPNTDGIHIHSSHNVIIKNTTIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSL 240
           PESSPNTDGIHIHSSHN+IIKNTTIGT GDDCVS GDYTS+VNISHI+CGPGHGISIGSL
Sbjct: 226 PESSPNTDGIHIHSSHNIIIKNTTIGT-GDDCVSTGDYTSHVNISHIKCGPGHGISIGSL 285

Query: 241 GRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQN 300
           G+SGNFVQVEDI VSN+YFKGTTN ARIKTWQ                            
Sbjct: 286 GKSGNFVQVEDISVSNIYFKGTTNRARIKTWQ---------------------------- 345

Query: 301 YGNLGGLSNEKQTGVHIANVSINNLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANS 356
                      Q+GV IANVSI NLFGTS+SNVAINLNCSRSVACTGILLKSIQL+S NS
Sbjct: 346 -----------QSGVRIANVSIKNLFGTSMSNVAINLNCSRSVACTGILLKSIQLQSVNS 392

[Close]

BLAST of MS011758 vs. NCBI nr
Match: ONH98318.1 (hypothetical protein PRUPE_7G242600 [Prunus persica])

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-130
Identity = 234/364 (64.29%), Postives = 282/364 (77.47%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW AAC+S+TE+ T+++P +  LL  PIV  GPCRA SI F+I G ++AP SPG 
Sbjct: 15  QAFLKAWNAACTSETENPTLIVPPKRFLL-NPIVFSGPCRAKSINFLIFGRLLAPDSPGA 74

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKII 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L         A+K++
Sbjct: 75  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPTAVKLV 134

Query: 121 SCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNT 180
           SC N+S+S IHF+NSSQTHVLI     + I+N++I APE SPNTDGIHI +SHNV+I N 
Sbjct: 135 SCKNSSISEIHFLNSSQTHVLIRDCDGINIENVLIEAPERSPNTDGIHISASHNVVITNA 194

Query: 181 TIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTT 240
            IGT GDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY +GTT
Sbjct: 195 IIGT-GDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYLQGTT 254

Query: 241 NGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSIN 300
           NGARIKTWQ+GRG +RRVTF++++F SV NP IIDQNY N  G   E +TGVHI +VS N
Sbjct: 255 NGARIKTWQVGRGYVRRVTFEHIFFGSVKNPIIIDQNYCNKSGACKEMETGVHITDVSFN 314

Query: 301 NLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEP 356
            L+GTS S VA+NLNCSRSVACT I LKSI L+SA +GQ VTS+C NAHG + GV+QP+P
Sbjct: 315 QLYGTSSSRVAMNLNCSRSVACTSIYLKSIYLRSALAGQNVTSNCTNAHGVASGVIQPDP 374

[Close]

BLAST of MS011758 vs. NCBI nr
Match: VVA10736.1 (PREDICTED: polygalacturonase [Prunus dulcis])

HSP 1 Score: 476.9 bits (1226), Expect = 1.6e-130
Identity = 232/364 (63.74%), Postives = 284/364 (78.02%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW AAC+S+TE+ T+++P++  LL  PIV  GPCRA +I F+I G ++AP SPG 
Sbjct: 15  QAFLKAWNAACTSETENPTLIVPRKRFLL-NPIVFSGPCRAENINFLIFGRLLAPDSPGA 74

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKII 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L         A+K++
Sbjct: 75  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPTAVKLV 134

Query: 121 SCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNT 180
           SC N+S+S IHF+NSSQTHVLI  S  + I+N++I APE SPNTDGIHI +SHNV+I N 
Sbjct: 135 SCKNSSISEIHFLNSSQTHVLIRDSDGINIENVLIEAPERSPNTDGIHISASHNVVITNA 194

Query: 181 TIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTT 240
            IGT GDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY +GTT
Sbjct: 195 IIGT-GDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYLQGTT 254

Query: 241 NGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSIN 300
           NGARIKTWQ+GRG +R+VTF++++F SV NP IIDQNY N  G   E +TGVHI +VS N
Sbjct: 255 NGARIKTWQVGRGYVRQVTFEHIFFGSVKNPIIIDQNYCNKSGACKEMETGVHITDVSFN 314

Query: 301 NLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEP 356
            L+GTS S VA+NLNCSRSVACT I LKSI ++SA +GQ VTS+C NAHG + GV+QP+P
Sbjct: 315 QLYGTSSSRVAMNLNCSRSVACTSIYLKSIYIRSALAGQNVTSNCTNAHGVASGVIQPDP 374

[Close]

BLAST of MS011758 vs. NCBI nr
Match: XP_020423968.1 (probable polygalacturonase At1g80170 [Prunus persica])

HSP 1 Score: 476.1 bits (1224), Expect = 2.7e-130
Identity = 233/364 (64.01%), Postives = 282/364 (77.47%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A LKAW AAC+S+TE+ T+++P +  LL  PIV  GPCRA SI F+I G ++AP SPG 
Sbjct: 49  EAFLKAWNAACTSETENPTLIVPPKRFLL-NPIVFSGPCRAKSINFLIFGRLLAPDSPGA 108

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKII 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L         A+K++
Sbjct: 109 WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPTAVKLV 168

Query: 121 SCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNT 180
           SC N+S+S IHF+NSSQTHVLI     + I+N++I APE SPNTDGIHI +SHNV+I N 
Sbjct: 169 SCKNSSISEIHFLNSSQTHVLIRDCDGINIENVLIEAPERSPNTDGIHISASHNVVITNA 228

Query: 181 TIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTT 240
            IGT GDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY +GTT
Sbjct: 229 IIGT-GDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYLQGTT 288

Query: 241 NGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSIN 300
           NGARIKTWQ+GRG +RRVTF++++F SV NP IIDQNY N  G   E +TGVHI +VS N
Sbjct: 289 NGARIKTWQVGRGYVRRVTFEHIFFGSVKNPIIIDQNYCNKSGACKEMETGVHITDVSFN 348

Query: 301 NLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEP 356
            L+GTS S VA+NLNCSRSVACT I LKSI L+SA +GQ VTS+C NAHG + GV+QP+P
Sbjct: 349 QLYGTSSSRVAMNLNCSRSVACTSIYLKSIYLRSALAGQNVTSNCTNAHGVASGVIQPDP 408

[Close]

BLAST of MS011758 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.3e-76
Identity = 151/360 (41.94%), Postives = 215/360 (59.72%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A   AWK ACSSK + + +L+P+ +T L++PI L GPC+A  +T  I G I+AP  P  
Sbjct: 70  KAFADAWKTACSSKVK-TRILVPENYTCLLRPIDLSGPCKAR-LTLQISGTIIAPNDPDV 129

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDH-------PDLAMKIISC 120
           W   ++ +WL F G+SRL V G   G ++G G  WW +SC+ +          A+    C
Sbjct: 130 WEGLNRRKWLYFHGLSRLTVEGG--GTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKC 189

Query: 121 NNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTI 180
            N  + N++ I+S Q H+ +   +RV I  L + AP +SPNTDGIHI  S  ++I NTT+
Sbjct: 190 KNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTV 249

Query: 181 GTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNG 240
            T GDDC+SI   ++ ++IS+I CGPGHGISIGSLG+S ++ +V DI V       T NG
Sbjct: 250 ST-GDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANG 309

Query: 241 ARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNL 300
            RIKTWQ G G + ++ F+N+   +V+NP IIDQ Y +       + + + I N+S  ++
Sbjct: 310 VRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHV 369

Query: 301 FGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPCL 354
            GTS S  AI ++CS S  C  ILL+ I L+ +N      S C  A+G S G V P PCL
Sbjct: 370 RGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPPPCL 424

[Close]

BLAST of MS011758 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.5e-71
Identity = 148/359 (41.23%), Postives = 212/359 (59.05%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW+A CS  T     ++P   T +++P+  +G C++  +   + G++VAP S G 
Sbjct: 41  QAFLKAWEAVCSG-TGDGQFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAP-SKGN 100

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLAMKIISCNNTSLSN 120
           W + D+ QW+ F  +  L + G   G+I+G+G+ WW    +     A+K  SCNN  LS 
Sbjct: 101 W-KGDKDQWILFTDIEGLVIEGD--GEINGQGSSWWEH--KGSRPTALKFRSCNNLRLSG 160

Query: 121 IHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTAGDDC 180
           +  ++S   H+ I     V I +L I APESSPNTDGI + +S NV+I++  I T GDDC
Sbjct: 161 LTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIAT-GDDC 220

Query: 181 VSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQ 240
           ++I   TSN++IS I CGPGHGISIGSLG+ G    VE++ V N  F+GT NGARIKTWQ
Sbjct: 221 IAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQ 280

Query: 241 IGRGNIRRVTFQNLYFESVNNPFIIDQNY--GNLGGLSNEKQTGVHIANVSINNLFGTSI 300
            G G  R +TF  +  ++V NP IIDQ Y  G+     + K + V ++ V  +N  GTS 
Sbjct: 281 GGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSK 340

Query: 301 SNVAINLNCSRSVACTGILLKSIQLKSAN--SGQKVTSSCLNAHGFSFGVVQPEPCLQI 356
           S   ++  CS  V CT I L+ +++++A+  SGQ     CLN  G S   V    CL++
Sbjct: 341 SEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLEL 391

[Close]

BLAST of MS011758 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 258.8 bits (660), Expect = 8.7e-68
Identity = 135/356 (37.92%), Postives = 209/356 (58.71%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A +KAW+AACSS T    ++ PK    ++K +   GPC+++ I F I+G I A  +P +
Sbjct: 87  KAFMKAWEAACSS-TGIVYIVAPKNRDYMLKAVTFSGPCKSSLIIFKIYGRIEAWENPSD 146

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL-------AMKIISC 120
           +   ++  W+ FE V+ L V G   G+IDG G  WW +SC+ +P L       A+  + C
Sbjct: 147 Y--KERRHWIVFENVNNLRVEGG--GRIDGNGHIWWPKSCKINPQLPCLGAPTAVTFVEC 206

Query: 121 NNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTI 180
           NN  +SNI   N+ Q H+     K V+  NL++T+P  SPNTDGIH+  + N++I+++ +
Sbjct: 207 NNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIV 266

Query: 181 GTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNG 240
            T GDDC+SI   + NV  + I CGPGHGISIGSLG   +   V ++ V+     GTTNG
Sbjct: 267 RT-GDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNG 326

Query: 241 ARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNL 300
            RIKTWQ G G  + + FQ++  ++V NP II+Q+Y +      E+++ V ++NV   N+
Sbjct: 327 VRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNI 386

Query: 301 FGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQP 350
            GTS   +A+   CS+++ C GI +++++L          +SC N    + G V P
Sbjct: 387 QGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKASCSNVKLDTRGNVSP 436

[Close]

BLAST of MS011758 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 5.7e-67
Identity = 146/356 (41.01%), Postives = 200/356 (56.18%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A  KAWKAACSS T S+ +L+PK++  LV+PI   GPC+ + +T  I+G I A     +
Sbjct: 106 KAFEKAWKAACSS-TSSAVLLVPKKN-YLVRPISFSGPCK-SGLTMQIYGTIEASDDRSD 165

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL-------AMKIISC 120
           + R D   WL F+ V  L V G   G I+G G  WW  SC+ +  L       A+     
Sbjct: 166 Y-RKDGRHWLVFDSVQNLRVEGG--GTINGNGKIWWQNSCKTNKALPCKDAPTALTFYKS 225

Query: 121 NNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTI 180
            +  + N+   N+ Q HV       V+  NL++TAPE+SPNTDGIH+  + N+ I +  I
Sbjct: 226 KHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVI 285

Query: 181 GTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNG 240
           GT GDDC+SI + +  V ++ I CGPGHGISIGSLG   +   V D+ V+     GTTNG
Sbjct: 286 GT-GDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNG 345

Query: 241 ARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNL 300
            RIKTWQ G G+   + FQN+   +V NP IIDQNY +      E+ + V + NV   N+
Sbjct: 346 VRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNI 405

Query: 301 FGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQP 350
            GT  SNVAI  +CS+   C GI+L+ + L+    G    + C N      GVV P
Sbjct: 406 KGTCASNVAITFDCSKRFPCQGIVLEDVDLE-IEGGAAAKALCNNVELSETGVVSP 453

[Close]

BLAST of MS011758 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 8.2e-66
Identity = 142/357 (39.78%), Postives = 204/357 (57.14%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA  KAWK ACS+    +T L+PK  T L+K    RGPC++    F I G + A T   +
Sbjct: 85  QAFKKAWKKACST-NGVTTFLVPKGKTYLLKSTRFRGPCKSLR-NFQILGTLSASTKRSD 144

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCR-------DHPDLAMKIISC 120
           +   D++ WL  E V+ L++ G   G I+G G  WW  SC+            A+ + + 
Sbjct: 145 Y--KDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNL 204

Query: 121 NNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTI 180
            N ++ N+   N+ Q  + I    +VE+ N+ ITAP  SPNTDGIHI ++ N+ + N+ I
Sbjct: 205 KNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDI 264

Query: 181 GTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNG 240
           GT GDDC+SI D T N+ I  + CGPGHGISIGSLG   +   V  I V    F  + NG
Sbjct: 265 GT-GDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNG 324

Query: 241 ARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNL 300
            RIKT+Q G G  + + FQN+  E+V NP IIDQ+Y +      ++++ V + NV   N+
Sbjct: 325 VRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNI 384

Query: 301 FGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPE 351
            GTS ++VAI LNCS    C GI+L+++++K        T+SC NA+  + G V P+
Sbjct: 385 SGTSATDVAITLNCSEKYPCQGIVLENVKIKGG------TASCKNANVKNQGTVSPK 429

[Close]

BLAST of MS011758 vs. ExPASy TrEMBL
Match: A0A6J1CMV4 (probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111013063 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 6.5e-199
Identity = 353/363 (97.25%), Postives = 353/363 (97.25%), Query Frame = 0


Query: 2   AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW 61
           AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW
Sbjct: 47  AILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEW 106

Query: 62  TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKIIS 121
           TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL         AMKIIS
Sbjct: 107 TRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLKGCTSLAPTAMKIIS 166

Query: 122 CNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTT 181
           CNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTT
Sbjct: 167 CNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTT 226

Query: 182 IGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTN 241
           IGT GDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTN
Sbjct: 227 IGT-GDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTN 286

Query: 242 GARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINN 301
           GARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINN
Sbjct: 287 GARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINN 346

Query: 302 LFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC 356
           LFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC
Sbjct: 347 LFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC 406

[Close]

BLAST of MS011758 vs. ExPASy TrEMBL
Match: A0A5A7V0D8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001680 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 7.5e-139
Identity = 263/387 (67.96%), Postives = 292/387 (75.45%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFM------------- 60
           QAI KAWKAAC+SKT+SSTVLIPKR T++VKPI+L GPCRA SITFM             
Sbjct: 46  QAISKAWKAACNSKTKSSTVLIPKRQTVMVKPILLSGPCRAKSITFMVKKNRDFPLMIKS 105

Query: 61  ---------------IHGEIVAPTSPGEWTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRG 120
                          I+G IVAPTSP EW  TD+S WLAF+G+SRL++TG+GIGKIDGRG
Sbjct: 106 FLEYYLSSGRCKLSQINGVIVAPTSPKEWAGTDKSLWLAFKGISRLSITGSGIGKIDGRG 165

Query: 121 TRWWSQSCRDHPDL----AMKIISCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITA 180
           T WW+QSCRDHP L    A KIISCN+T+LSN+HFINSSQ HVL+M  +R EIKNLVITA
Sbjct: 166 TGWWNQSCRDHPSLPLLQATKIISCNDTNLSNLHFINSSQAHVLLMECRRTEIKNLVITA 225

Query: 181 PESSPNTDGIHIHSSHNVIIKNTTIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSL 240
           PESSPNTDGIHIHSSHN+IIKNTTIGT GDDCVS GDYTS+VNISHI+CGPGHGISIGSL
Sbjct: 226 PESSPNTDGIHIHSSHNIIIKNTTIGT-GDDCVSTGDYTSHVNISHIKCGPGHGISIGSL 285

Query: 241 GRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQN 300
           G+SGNFVQVEDI VSN+YFKGTTN ARIKTWQ                            
Sbjct: 286 GKSGNFVQVEDISVSNIYFKGTTNRARIKTWQ---------------------------- 345

Query: 301 YGNLGGLSNEKQTGVHIANVSINNLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANS 356
                      Q+GV IANVSI NLFGTS+SNVAINLNCSRSVACTGILLKSIQL+S NS
Sbjct: 346 -----------QSGVRIANVSIKNLFGTSMSNVAINLNCSRSVACTGILLKSIQLQSVNS 392

[Close]

BLAST of MS011758 vs. ExPASy TrEMBL
Match: M5WCW5 (Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa021427mg PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.2e-133
Identity = 233/355 (65.63%), Postives = 282/355 (79.44%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A LKAW AAC+S+TE+ T+++P +  LL  PIV  GPCRA SI F+I G ++AP SPG 
Sbjct: 33  EAFLKAWNAACTSETENPTLIVPPKRFLL-NPIVFSGPCRAKSINFLIFGRLLAPDSPGA 92

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLAMKIISCNNTSLSN 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP LA+K++SC N+S+S 
Sbjct: 93  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLAVKLVSCKNSSISE 152

Query: 121 IHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTAGDDC 180
           IHF+NSSQTHVLI     + I+N++I APE SPNTDGIHI +SHNV+I N  IGT GDDC
Sbjct: 153 IHFLNSSQTHVLIRDCDGINIENVLIEAPERSPNTDGIHISASHNVVITNAIIGT-GDDC 212

Query: 181 VSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQ 240
           VSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY +GTTNGARIKTWQ
Sbjct: 213 VSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYLQGTTNGARIKTWQ 272

Query: 241 IGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNLFGTSISN 300
           +GRG +RRVTF++++F SV NP IIDQNY N  G   E +TGVHI +VS N L+GTS S 
Sbjct: 273 VGRGYVRRVTFEHIFFGSVKNPIIIDQNYCNKSGACKEMETGVHITDVSFNQLYGTSSSR 332

Query: 301 VAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPCLQI 356
           VA+NLNCSRSVACT I LKSI L+SA +GQ VTS+C NAHG + GV+QP+PCLQI
Sbjct: 333 VAMNLNCSRSVACTSIYLKSIYLRSALAGQNVTSNCTNAHGVASGVIQPDPCLQI 383

[Close]

BLAST of MS011758 vs. ExPASy TrEMBL
Match: A0A251NG61 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G242600 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.8e-131
Identity = 234/364 (64.29%), Postives = 282/364 (77.47%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW AAC+S+TE+ T+++P +  LL  PIV  GPCRA SI F+I G ++AP SPG 
Sbjct: 15  QAFLKAWNAACTSETENPTLIVPPKRFLL-NPIVFSGPCRAKSINFLIFGRLLAPDSPGA 74

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKII 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L         A+K++
Sbjct: 75  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPTAVKLV 134

Query: 121 SCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNT 180
           SC N+S+S IHF+NSSQTHVLI     + I+N++I APE SPNTDGIHI +SHNV+I N 
Sbjct: 135 SCKNSSISEIHFLNSSQTHVLIRDCDGINIENVLIEAPERSPNTDGIHISASHNVVITNA 194

Query: 181 TIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTT 240
            IGT GDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY +GTT
Sbjct: 195 IIGT-GDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYLQGTT 254

Query: 241 NGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSIN 300
           NGARIKTWQ+GRG +RRVTF++++F SV NP IIDQNY N  G   E +TGVHI +VS N
Sbjct: 255 NGARIKTWQVGRGYVRRVTFEHIFFGSVKNPIIIDQNYCNKSGACKEMETGVHITDVSFN 314

Query: 301 NLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEP 356
            L+GTS S VA+NLNCSRSVACT I LKSI L+SA +GQ VTS+C NAHG + GV+QP+P
Sbjct: 315 QLYGTSSSRVAMNLNCSRSVACTSIYLKSIYLRSALAGQNVTSNCTNAHGVASGVIQPDP 374

[Close]

BLAST of MS011758 vs. ExPASy TrEMBL
Match: A0A5E4E632 (PREDICTED: polygalacturonase OS=Prunus dulcis OX=3755 GN=ALMOND_2B007689 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 7.5e-131
Identity = 232/364 (63.74%), Postives = 284/364 (78.02%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW AAC+S+TE+ T+++P++  LL  PIV  GPCRA +I F+I G ++AP SPG 
Sbjct: 15  QAFLKAWNAACTSETENPTLIVPRKRFLL-NPIVFSGPCRAENINFLIFGRLLAPDSPGA 74

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL---------AMKII 120
           W   D SQWLAF GVS LN+ G   G+I+GRG  WW QSCRDHP L         A+K++
Sbjct: 75  WKELDPSQWLAFNGVSGLNIAGP--GRINGRGKAWWDQSCRDHPRLVGCSTLAPTAVKLV 134

Query: 121 SCNNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNT 180
           SC N+S+S IHF+NSSQTHVLI  S  + I+N++I APE SPNTDGIHI +SHNV+I N 
Sbjct: 135 SCKNSSISEIHFLNSSQTHVLIRDSDGINIENVLIEAPERSPNTDGIHISASHNVVITNA 194

Query: 181 TIGTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTT 240
            IGT GDDCVSIGD+TSN+ IS+++CGPGHGISIGSLGRSGNFVQVE+I VS VY +GTT
Sbjct: 195 IIGT-GDDCVSIGDHTSNIVISYVKCGPGHGISIGSLGRSGNFVQVENIHVSKVYLQGTT 254

Query: 241 NGARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSIN 300
           NGARIKTWQ+GRG +R+VTF++++F SV NP IIDQNY N  G   E +TGVHI +VS N
Sbjct: 255 NGARIKTWQVGRGYVRQVTFEHIFFGSVKNPIIIDQNYCNKSGACKEMETGVHITDVSFN 314

Query: 301 NLFGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEP 356
            L+GTS S VA+NLNCSRSVACT I LKSI ++SA +GQ VTS+C NAHG + GV+QP+P
Sbjct: 315 QLYGTSSSRVAMNLNCSRSVACTSIYLKSIYIRSALAGQNVTSNCTNAHGVASGVIQPDP 374

[Close]

BLAST of MS011758 vs. TAIR 10
Match: AT1G80140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.3e-79
Identity = 155/337 (45.99%), Postives = 209/337 (62.02%), Query Frame = 0


Query: 19  TVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGEWTRTDQS--QWLAFEGVS 78
           +V +P     L+  +   GPC    + F+I GE++A + P +W   +     WL F+ V 
Sbjct: 3   SVNVPAGKVFLLNSLHFTGPCIPKPLLFIIDGEMIAQSDPQKWGNGENGVIPWLIFDQVD 62

Query: 79  RLNVTGTGIGKIDGRGTRWWSQSCRDHPDLAMKIISCNNTSLSNIHFINSSQTHVLIMGS 138
            L + G G+  +DG+G  WW   CRDHP   M   +C N +L ++ F NS+QTHVL+MGS
Sbjct: 63  GLAIVGRGL--LDGQGKSWWDIHCRDHPGPMMTFSNCGNVTLKSLRFRNSAQTHVLVMGS 122

Query: 139 KRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTAGDDCVSIGDYTSNVNISHIR 198
           + V I ++ IT+PE+SPNTDGIHI SS  V I ++ I T GDDCVSIGD  +N+N++ + 
Sbjct: 123 QNVYIDDIKITSPEASPNTDGIHITSSTAVSINHSDIAT-GDDCVSIGDQVNNLNVTFMN 182

Query: 199 CGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQIGRGNIRRVTFQNLYF 258
           CGPGHG+SIGSLGR G  V VE+IRVS+V F GTTNGARIKTW  G G +R + F ++ F
Sbjct: 183 CGPGHGVSIGSLGRGGTEVTVENIRVSHVNFTGTTNGARIKTWPGGTGYVRGIEFFDIRF 242

Query: 259 ESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNLFGTSISNVAINLNCS-RSVACTG 318
            SV NP IIDQ YG       E   GVHI  V    + GTS + VA+ L CS  SV C+ 
Sbjct: 243 SSVQNPIIIDQFYG-CAPTCVETMKGVHIEKVRYMKMSGTSATKVAMKLECSGESVPCSN 302

Query: 319 ILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPC 353
           + ++ I L  A+    V+S C  AHG + G+++P  C
Sbjct: 303 LFMRDIDLSPADGIDSVSSLCSFAHGSAQGIIRPLSC 335

[Close]

BLAST of MS011758 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.6e-77
Identity = 151/360 (41.94%), Postives = 215/360 (59.72%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A   AWK ACSSK + + +L+P+ +T L++PI L GPC+A  +T  I G I+AP  P  
Sbjct: 70  KAFADAWKTACSSKVK-TRILVPENYTCLLRPIDLSGPCKAR-LTLQISGTIIAPNDPDV 129

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDH-------PDLAMKIISC 120
           W   ++ +WL F G+SRL V G   G ++G G  WW +SC+ +          A+    C
Sbjct: 130 WEGLNRRKWLYFHGLSRLTVEGG--GTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKC 189

Query: 121 NNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTI 180
            N  + N++ I+S Q H+ +   +RV I  L + AP +SPNTDGIHI  S  ++I NTT+
Sbjct: 190 KNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTV 249

Query: 181 GTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNG 240
            T GDDC+SI   ++ ++IS+I CGPGHGISIGSLG+S ++ +V DI V       T NG
Sbjct: 250 ST-GDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANG 309

Query: 241 ARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNL 300
            RIKTWQ G G + ++ F+N+   +V+NP IIDQ Y +       + + + I N+S  ++
Sbjct: 310 VRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHV 369

Query: 301 FGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQPEPCL 354
            GTS S  AI ++CS S  C  ILL+ I L+ +N      S C  A+G S G V P PCL
Sbjct: 370 RGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPPPCL 424

[Close]

BLAST of MS011758 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.6e-72
Identity = 148/359 (41.23%), Postives = 212/359 (59.05%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW+A CS  T     ++P   T +++P+  +G C++  +   + G++VAP S G 
Sbjct: 41  QAFLKAWEAVCSG-TGDGQFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAP-SKGN 100

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLAMKIISCNNTSLSN 120
           W + D+ QW+ F  +  L + G   G+I+G+G+ WW    +     A+K  SCNN  LS 
Sbjct: 101 W-KGDKDQWILFTDIEGLVIEGD--GEINGQGSSWWEH--KGSRPTALKFRSCNNLRLSG 160

Query: 121 IHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTAGDDC 180
           +  ++S   H+ I     V I +L I APESSPNTDGI + +S NV+I++  I T GDDC
Sbjct: 161 LTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIAT-GDDC 220

Query: 181 VSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQ 240
           ++I   TSN++IS I CGPGHGISIGSLG+ G    VE++ V N  F+GT NGARIKTWQ
Sbjct: 221 IAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQ 280

Query: 241 IGRGNIRRVTFQNLYFESVNNPFIIDQNY--GNLGGLSNEKQTGVHIANVSINNLFGTSI 300
            G G  R +TF  +  ++V NP IIDQ Y  G+     + K + V ++ V  +N  GTS 
Sbjct: 281 GGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSK 340

Query: 301 SNVAINLNCSRSVACTGILLKSIQLKSAN--SGQKVTSSCLNAHGFSFGVVQPEPCLQI 356
           S   ++  CS  V CT I L+ +++++A+  SGQ     CLN  G S   V    CL++
Sbjct: 341 SEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLEL 391

[Close]

BLAST of MS011758 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 5.1e-71
Identity = 147/359 (40.95%), Postives = 210/359 (58.50%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           QA LKAW+A CS  T     ++P   T +++P+  +G C++  +   + G++VAP S G 
Sbjct: 40  QAFLKAWEAVCSG-TGDGQFVVPAGMTFMLQPLKFQGSCKSTPVFVQMLGKLVAP-SKGN 99

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDLAMKIISCNNTSLSN 120
           W + D+ QW+ F  +  L + G   G+I+G+G+ WW     +H        SCNN  LS 
Sbjct: 100 W-KGDKDQWILFTDIEGLVIEGD--GEINGQGSSWW-----EHKGSRPTFRSCNNLRLSG 159

Query: 121 IHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTIGTAGDDC 180
           +  ++S   H+ I     V I +L I APESSPNTDGI + +S NV+I++  I T GDDC
Sbjct: 160 LTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIAT-GDDC 219

Query: 181 VSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNGARIKTWQ 240
           ++I   TSN++IS I CGPGHGISIGSLG+ G    VE++ V N  F+GT NGARIKTWQ
Sbjct: 220 IAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQ 279

Query: 241 IGRGNIRRVTFQNLYFESVNNPFIIDQNY--GNLGGLSNEKQTGVHIANVSINNLFGTSI 300
            G G  R +TF  +  ++V NP IIDQ Y  G+     + K + V ++ V  +N  GTS 
Sbjct: 280 GGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSK 339

Query: 301 SNVAINLNCSRSVACTGILLKSIQLKSAN--SGQKVTSSCLNAHGFSFGVVQPEPCLQI 356
           S   ++  CS  V CT I L+ +++++A+  SGQ     CLN  G S   V    CL++
Sbjct: 340 SEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLEL 387

[Close]

BLAST of MS011758 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 6.2e-69
Identity = 135/356 (37.92%), Postives = 209/356 (58.71%), Query Frame = 0


Query: 1   QAILKAWKAACSSKTESSTVLIPKRHTLLVKPIVLRGPCRANSITFMIHGEIVAPTSPGE 60
           +A +KAW+AACSS T    ++ PK    ++K +   GPC+++ I F I+G I A  +P +
Sbjct: 87  KAFMKAWEAACSS-TGIVYIVAPKNRDYMLKAVTFSGPCKSSLIIFKIYGRIEAWENPSD 146

Query: 61  WTRTDQSQWLAFEGVSRLNVTGTGIGKIDGRGTRWWSQSCRDHPDL-------AMKIISC 120
           +   ++  W+ FE V+ L V G   G+IDG G  WW +SC+ +P L       A+  + C
Sbjct: 147 Y--KERRHWIVFENVNNLRVEGG--GRIDGNGHIWWPKSCKINPQLPCLGAPTAVTFVEC 206

Query: 121 NNTSLSNIHFINSSQTHVLIMGSKRVEIKNLVITAPESSPNTDGIHIHSSHNVIIKNTTI 180
           NN  +SNI   N+ Q H+     K V+  NL++T+P  SPNTDGIH+  + N++I+++ +
Sbjct: 207 NNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIV 266

Query: 181 GTAGDDCVSIGDYTSNVNISHIRCGPGHGISIGSLGRSGNFVQVEDIRVSNVYFKGTTNG 240
            T GDDC+SI   + NV  + I CGPGHGISIGSLG   +   V ++ V+     GTTNG
Sbjct: 267 RT-GDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNG 326

Query: 241 ARIKTWQIGRGNIRRVTFQNLYFESVNNPFIIDQNYGNLGGLSNEKQTGVHIANVSINNL 300
            RIKTWQ G G  + + FQ++  ++V NP II+Q+Y +      E+++ V ++NV   N+
Sbjct: 327 VRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNI 386

Query: 301 FGTSISNVAINLNCSRSVACTGILLKSIQLKSANSGQKVTSSCLNAHGFSFGVVQP 350
            GTS   +A+   CS+++ C GI +++++L          +SC N    + G V P
Sbjct: 387 QGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKASCSNVKLDTRGNVSP 436

The following BLAST results are available for this feature:

BLAST of MS011758 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143115.1	1.3e-198	97.25	probable polygalacturonase At1g80170 [Momordica charantia]	[more]
KAA0061603.1	1.6e-138	67.96	putative polygalacturonase [Cucumis melo var. makuwa]	[more]
ONH98318.1	1.2e-130	64.29	hypothetical protein PRUPE_7G242600 [Prunus persica]	[more]
VVA10736.1	1.6e-130	63.74	PREDICTED: polygalacturonase [Prunus dulcis]	[more]
XP_020423968.1	2.7e-130	64.01	probable polygalacturonase At1g80170 [Prunus persica]	[more]

BLAST of MS011758 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AJ5	2.3e-76	41.94	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
Q9LW07	6.5e-71	41.23	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q9SFB7	8.7e-68	37.92	Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2	[more]
P35336	5.7e-67	41.01	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]
O23147	8.2e-66	39.78	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]

BLAST of MS011758 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMV4	6.5e-199	97.25	probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A5A7V0D8	7.5e-139	67.96	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
M5WCW5	6.2e-133	65.63	Uncharacterized protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa021427m...	[more]
A0A251NG61	5.8e-131	64.29	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G242600 PE=3 SV=1	[more]
A0A5E4E632	7.5e-131	63.74	PREDICTED: polygalacturonase OS=Prunus dulcis OX=3755 GN=ALMOND_2B007689 PE=3 SV...	[more]

BLAST of MS011758 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80140.1	1.3e-79	45.99	Pectin lyase-like superfamily protein	[more]
AT1G80170.1	1.6e-77	41.94	Pectin lyase-like superfamily protein	[more]
AT3G15720.1	4.6e-72	41.23	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	5.1e-71	40.95	Pectin lyase-like superfamily protein	[more]
AT3G07970.1	6.2e-69	37.92	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 187..207 e-value: 5000.0 score: 2.3 coord: 246..287 e-value: 6200.0 score: 1.6 coord: 163..185 e-value: 160.0 score: 11.1 coord: 217..238 e-value: 120.0 score: 11.5 coord: 136..162 e-value: 310.0 score: 10.1
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 14..340 e-value: 5.8E-77 score: 258.9
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 194..207
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..352 e-value: 8.1E-99 score: 333.4
None	No IPR available	PANTHER	PTHR31375:SF229	POLYGALACTURONASE-LIKE	coord: 1..345
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..345
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 1..353

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011758.1	MS011758.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity