MS011718 (gene) Bitter gourd (TR) v1

Overview
NameMS011718
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMonogalactosyldiacylglycerol synthase
Locationscaffold11: 157735 .. 160861 (+)
RNA-Seq ExpressionMS011718
SyntenyMS011718
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTTGCAACGCGGAAGTCTGTTACAGAGAAGGTGTTTCAGAGGGTGGGAGGGCATTATCAATGGGGTAGTAGTCGTAGATCATGTCGGAATGGTTGCAGAGAGGAGGATTATGACGACGATGGGAATTTGGAATTGGTTCAGATTGGGGCGCAGAGGACGAAGAATGTTCTTATTTTGATGAGTGATACAGGGGGTGGCCACAGGGCATCTGCGGAGGCGATTCGGGATGCGTTTCAGATTGAATTTGGAGACGAGTATCAAGTAACTCAATGATTTTCTCTTCTGGGTTTTGGAATTTTTCCTAACTTACACCTTTGATTCATGTTCGTTTGTTACCTTAAGCTGTGTTTAATCGTCAGATTTGTTGATTATTTGCTGAAAATCTTGTTGAAGATGGTCGGTTTTGGCTTCTAATTCACCTCTCTGTTTTATCTCGCCTATGTTTTACGTGGAAGTATTGGAAACGTGCCATTTGATGTTTGTCCTTAAAGTATAAGATCTTGCAATTTACACTTCATAACTTCTTGGTTACTCTGTCTGAGCCAAATTTGAACAGTTCCCAATACGTTTGGCCAATTAAAAAGGCAGAAACGTGTTTTGTTTTTATCATGGGAATTATCTTATGTAATTCTTGCTTCTCGCTGTGTACAAGTGATCTATTAGAAGAGATGAATGATGCTGGCTCTCGGTTTCTCACTTCTTTTTTCTTCTTCTTCTATCTTTCTTCTTTTTCAGATATTTGTGAAGGATGTTTGGAAAGAGTACACGGGTTGGCCTTTGAATGACATGGAGGGATCATACAAATTTATGGTAAAACATGTCCAGCTATGGAAGGTTGCATTTCATAGCACCTCCCCTCGGTGGATTCACTCACTCTATCTTGCTGCAATTGCCGCCTACTATGCAAAGTACATATAACCTTCTCTTGCACACTTTCTTCGGCTAATCTTTTCAATATATTCTCGAACACTATCACCATACTCTACTATTATTACAAGATTGGACAAGTGAGGTATGAATATCAAATGAGAAGGAAGTAAGAAATACATTGCTGAACTTTGAACTGATGTTCGAACGCTAAAACCAAAAACTCGAATCGTTGGGGTAATGTTTTGAATATATTTTCTAACACATTCTTATGATTTTCTTCCCCTTTTGACAATGATGTAGATCGTATCATATTTGAGCGAGCCATGGTGATTCATCATTTCAAACTCGTTTTATTAACCGTTTGACTAACTAAAACTTAATTCTAGTATGGGCTTGAGATGTCTGGGGATAATACTGTTCAAGTTCTCAGGTGGAAACTTAATAATTTAAAATTATTTGTCCCCCTAAAGCCTCTCTGAGGATTAGTTAGACGATCACGAGCATAATGCAAGTAGTGGAGTCGGTTATAGAACAATGTTCTTGCTAGAGATCTTGACTATGAACCATTGATGTTATGAGGTAACTTACATTGTGTGTAGAATGTAGAATGGTGTTTCTAACTTTGTAGTACCTTTTTTTCTGTTGAAGGGAGGTGGAGGCTGGTCTGATGGAGTACAGGCCAGATATTATCATCAGTGTTCATCCTCTGATGCAACATATTCCCTTATGGGTTCTGAAATGGCAAGGCCTTCAGAAGAAAGTGGTGTTTGTTACAGTCATTACAGACCTAAACACCTGCCATCCAACATGGTTAGTTTGCAAATTACCATTTGATTCCCCATTTTCAACTAATACCATTATCTTTTAGTTCTTTTTAGCTGACATTTGAACACCATATAAATAAATCCAGGTTTCATCCTGGAGTCAACAGATGTTACTGCCCATCAGATGCAGTAGCCAAGAGAGCCTTACTCGATGGCCTCGAAGAGTCCCAAGTTCGTGTCTACGGCCTACCGATCAGGCCATCCTTTGCTCGGGCGGTACTCCAGAAGGTAGATCTTTTCATTTTTATACAGCACAAACATGTGATTTAGTTTGATGGAGGTCTGATTCGCTCATGGTGGTTGTTCGGTTTCGTTTATTTTCCTACACAGGGTCAACTAAGACAAGAACTTGAAATGGATCCAGATCTACCAGCAGTGCTGCTGATGGGAGGAGGTGAAGGGATGGGTCCTGTGAAGGAAACAGCAAAAGCTCTTGCAGAATCCCTTTTTGATAAGAACAGTGAGAAGCCCATCGGGCAAATTGTTGTTATTTGTGGAAGAAACAAAGCTTTAGCATCTTCTCTTGAAGCTGGGGAATGGAAGATCCCAGTTAAGGTCAGTGGAAGTCCATTCCTTTTCCTTTTCTTTTGGCCTTGGGGCATCCATTACATCTCCTTTTTTCTCTCTTTTGCAGGTGAGAGGTTTCGAAAAACAGATGGAGAAATGGATGGGTGCTTGTGACTGTATAATAACCAAAGTATGTCTTTGCTTTATTTCTTGATATGCTCTTAAAAATTTGTTGTTTATAATCAAATGTATGGATACTTGAGCTAAAACAAATTTGTGTTTAAGTGGTCTGTGAATTTAAAACAACGTCTAATAAGTTTATGACCTATTAGATATAAAATGAAAGTTCAAACACTTGTTAGATAATTTTTAAAGTTTAAGAATCTGATAGACACAATTCTAGAAGTTTGAAGACTAAACTTGTAACGTAACCTATTGATACATACATGTGACGTATAGATAAACAAAATGGATAATGGATCATGTTGATTTTTTCGTTTATAGGCCGGTCCTGGAACAATTGCAGAAGCTTTGATTCGAGGGCTTCCGATTATTCTCAACGACTACATACCCGGGCAAGTAAGTGATTAAACTTGACACTTGCATCAAGCTTTAGACCACATTTACCATAACAAACAAATTGCAAAACTTTTCTCCTTTAGGAAAAAGGAAATGTTCCATATGTGGTAGACAATGGAGCTGGAGTGTTCACCAGAAGTCCCAAAGAAACAGCCAGAATCGTTGCTGAATGGTTCAGTAGAAATACAGAAGAACTCCACACAATGTCGCAGAATGCTCTCAAACTAGCACAACCTGAAGCCGTCTTCGACATTGTCCGGGACCTCCACGAGCTCGCCTGCCAGCGCGGTCCAATGGCGAACATCCCGTACATGTTGACATCGTCGTTTACAGGCCTAATC

mRNA sequence

ATGTCTGTTGCAACGCGGAAGTCTGTTACAGAGAAGGTGTTTCAGAGGGTGGGAGGGCATTATCAATGGGGTAGTAGTCGTAGATCATGTCGGAATGGTTGCAGAGAGGAGGATTATGACGACGATGGGAATTTGGAATTGGTTCAGATTGGGGCGCAGAGGACGAAGAATGTTCTTATTTTGATGAGTGATACAGGGGGTGGCCACAGGGCATCTGCGGAGGCGATTCGGGATGCGTTTCAGATTGAATTTGGAGACGAGTATCAAATATTTGTGAAGGATGTTTGGAAAGAGTACACGGGTTGGCCTTTGAATGACATGGAGGGATCATACAAATTTATGGTAAAACATGTCCAGCTATGGAAGGTTGCATTTCATAGCACCTCCCCTCGGTGGATTCACTCACTCTATCTTGCTGCAATTGCCGCCTACTATGCAAAGGAGGTGGAGGCTGGTCTGATGGAGTACAGGCCAGATATTATCATCAGTGTTCATCCTCTGATGCAACATATTCCCTTATGGGTTCTGAAATGGCAAGGCCTTCAGAAGAAAGTGGTGTTTGTTACAGTCATTACAGACCTAAACACCTGCCATCCAACATGGTTTCATCCTGGAGTCAACAGATGTTACTGCCCATCAGATGCAGTAGCCAAGAGAGCCTTACTCGATGGCCTCGAAGAGTCCCAAGTTCGTGTCTACGGCCTACCGATCAGGCCATCCTTTGCTCGGGCGGTACTCCAGAAGGGTCAACTAAGACAAGAACTTGAAATGGATCCAGATCTACCAGCAGTGCTGCTGATGGGAGGAGGTGAAGGGATGGGTCCTGTGAAGGAAACAGCAAAAGCTCTTGCAGAATCCCTTTTTGATAAGAACAGTGAGAAGCCCATCGGGCAAATTGTTGTTATTTGTGGAAGAAACAAAGCTTTAGCATCTTCTCTTGAAGCTGGGGAATGGAAGATCCCAGTTAAGGTGAGAGGTTTCGAAAAACAGATGGAGAAATGGATGGGTGCTTGTGACTGTATAATAACCAAAGCCGGTCCTGGAACAATTGCAGAAGCTTTGATTCGAGGGCTTCCGATTATTCTCAACGACTACATACCCGGGCAAGAAAAAGGAAATGTTCCATATGTGGTAGACAATGGAGCTGGAGTGTTCACCAGAAGTCCCAAAGAAACAGCCAGAATCGTTGCTGAATGGTTCAGTAGAAATACAGAAGAACTCCACACAATGTCGCAGAATGCTCTCAAACTAGCACAACCTGAAGCCGTCTTCGACATTGTCCGGGACCTCCACGAGCTCGCCTGCCAGCGCGGTCCAATGGCGAACATCCCGTACATGTTGACATCGTCGTTTACAGGCCTAATC

Coding sequence (CDS)

ATGTCTGTTGCAACGCGGAAGTCTGTTACAGAGAAGGTGTTTCAGAGGGTGGGAGGGCATTATCAATGGGGTAGTAGTCGTAGATCATGTCGGAATGGTTGCAGAGAGGAGGATTATGACGACGATGGGAATTTGGAATTGGTTCAGATTGGGGCGCAGAGGACGAAGAATGTTCTTATTTTGATGAGTGATACAGGGGGTGGCCACAGGGCATCTGCGGAGGCGATTCGGGATGCGTTTCAGATTGAATTTGGAGACGAGTATCAAATATTTGTGAAGGATGTTTGGAAAGAGTACACGGGTTGGCCTTTGAATGACATGGAGGGATCATACAAATTTATGGTAAAACATGTCCAGCTATGGAAGGTTGCATTTCATAGCACCTCCCCTCGGTGGATTCACTCACTCTATCTTGCTGCAATTGCCGCCTACTATGCAAAGGAGGTGGAGGCTGGTCTGATGGAGTACAGGCCAGATATTATCATCAGTGTTCATCCTCTGATGCAACATATTCCCTTATGGGTTCTGAAATGGCAAGGCCTTCAGAAGAAAGTGGTGTTTGTTACAGTCATTACAGACCTAAACACCTGCCATCCAACATGGTTTCATCCTGGAGTCAACAGATGTTACTGCCCATCAGATGCAGTAGCCAAGAGAGCCTTACTCGATGGCCTCGAAGAGTCCCAAGTTCGTGTCTACGGCCTACCGATCAGGCCATCCTTTGCTCGGGCGGTACTCCAGAAGGGTCAACTAAGACAAGAACTTGAAATGGATCCAGATCTACCAGCAGTGCTGCTGATGGGAGGAGGTGAAGGGATGGGTCCTGTGAAGGAAACAGCAAAAGCTCTTGCAGAATCCCTTTTTGATAAGAACAGTGAGAAGCCCATCGGGCAAATTGTTGTTATTTGTGGAAGAAACAAAGCTTTAGCATCTTCTCTTGAAGCTGGGGAATGGAAGATCCCAGTTAAGGTGAGAGGTTTCGAAAAACAGATGGAGAAATGGATGGGTGCTTGTGACTGTATAATAACCAAAGCCGGTCCTGGAACAATTGCAGAAGCTTTGATTCGAGGGCTTCCGATTATTCTCAACGACTACATACCCGGGCAAGAAAAAGGAAATGTTCCATATGTGGTAGACAATGGAGCTGGAGTGTTCACCAGAAGTCCCAAAGAAACAGCCAGAATCGTTGCTGAATGGTTCAGTAGAAATACAGAAGAACTCCACACAATGTCGCAGAATGCTCTCAAACTAGCACAACCTGAAGCCGTCTTCGACATTGTCCGGGACCTCCACGAGCTCGCCTGCCAGCGCGGTCCAATGGCGAACATCCCGTACATGTTGACATCGTCGTTTACAGGCCTAATC

Protein sequence

MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQPEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI
Homology
BLAST of MS011718 vs. NCBI nr
Match: XP_022143037.1 (monogalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 928.7 bits (2399), Expect = 1.9e-266
Identity = 451/455 (99.12%), Postives = 452/455 (99.34%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLI 60
           MSVATRKSVTEKVFQRVGGHYQ GSSRRSC NGCREEDYDDDGNLELVQIGAQRTKNVLI
Sbjct: 74  MSVATRKSVTEKVFQRVGGHYQCGSSRRSCGNGCREEDYDDDGNLELVQIGAQRTKNVLI 133

Query: 61  LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 120
           LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL
Sbjct: 134 LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 193

Query: 121 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 180
           WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG
Sbjct: 194 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 253

Query: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 240
           LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS
Sbjct: 254 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 313

Query: 241 FARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 300
           FARAVLQKGQLR+ELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV
Sbjct: 314 FARAVLQKGQLREELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 373

Query: 301 VICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360
           VICGRNKALASSLEAGEWKI VKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI
Sbjct: 374 VICGRNKALASSLEAGEWKISVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 433

Query: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 420
           ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP
Sbjct: 434 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 493

Query: 421 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI
Sbjct: 494 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 528

BLAST of MS011718 vs. NCBI nr
Match: XP_038889391.1 (monogalactosyldiacylglycerol synthase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 872.5 bits (2253), Expect = 1.6e-249
Identity = 419/456 (91.89%), Postives = 438/456 (96.05%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDY-DDDGNLELVQIGAQRTKNVL 60
           MSVAT +S+TEKVFQRVGGHYQW SS + C N  REEDY DDDG LE+VQIGA+RTKNVL
Sbjct: 1   MSVATPRSITEKVFQRVGGHYQWSSSNKICGNDFREEDYDDDDGTLEMVQIGAERTKNVL 60

Query: 61  ILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQ 120
           ILMSDTGGGHRASAEAIRDAF IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHVQ
Sbjct: 61  ILMSDTGGGHRASAEAIRDAFHIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHVQ 120

Query: 121 LWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQ 180
           LWKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKWQ
Sbjct: 121 LWKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKWQ 180

Query: 181 GLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRP 240
           GLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLDG+EESQVRVYGLPIRP
Sbjct: 181 GLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDGIEESQVRVYGLPIRP 240

Query: 241 SFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQI 300
           SFARAVLQK QLR+ELEMDPDLPAVLLMGGGEGMGPVK+TAKALAESLFDK +EKPIGQ+
Sbjct: 241 SFARAVLQKDQLREELEMDPDLPAVLLMGGGEGMGPVKKTAKALAESLFDKENEKPIGQL 300

Query: 301 VVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLP 360
           V+ICGRNK LASSLEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLP
Sbjct: 301 VIICGRNKGLASSLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLP 360

Query: 361 IILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQ 420
           IILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA+IVAEWFS  TE+LHTMSQNALKLAQ
Sbjct: 361 IILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAKIVAEWFSTKTEQLHTMSQNALKLAQ 420

Query: 421 PEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           PEAVFDIVRD+H+LACQRGPMA+IPYMLTSSFT LI
Sbjct: 421 PEAVFDIVRDIHDLACQRGPMAHIPYMLTSSFTALI 456

BLAST of MS011718 vs. NCBI nr
Match: XP_008457220.1 (PREDICTED: monogalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 867.5 bits (2240), Expect = 5.2e-248
Identity = 417/457 (91.25%), Postives = 438/457 (95.84%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDY--DDDGNLELVQIGAQRTKNV 60
           MSVAT +S+TEKVFQRVGGHYQW S+ +   N CREEDY  DDDG LE+VQIGA+RTKNV
Sbjct: 1   MSVATPRSITEKVFQRVGGHYQWSSTHKIFGNDCREEDYYDDDDGTLEMVQIGAERTKNV 60

Query: 61  LILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHV 120
           LILMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHV
Sbjct: 61  LILMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHV 120

Query: 121 QLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKW 180
           QLWKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKW
Sbjct: 121 QLWKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKW 180

Query: 181 QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIR 240
           QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLDG+EESQVRVYGLPIR
Sbjct: 181 QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDGIEESQVRVYGLPIR 240

Query: 241 PSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQ 300
           PSFARAVLQK QLR+ELEMD DLPAVLLMGGGEGMGPVK+TAKALAESLFDK +EKPIGQ
Sbjct: 241 PSFARAVLQKDQLREELEMDLDLPAVLLMGGGEGMGPVKKTAKALAESLFDKENEKPIGQ 300

Query: 301 IVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL 360
           +V+ICGRNKALAS+LEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL
Sbjct: 301 LVIICGRNKALASTLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL 360

Query: 361 PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLA 420
           PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA+IVAEWFS  TE+ HTMSQNALKLA
Sbjct: 361 PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAKIVAEWFSTKTEQFHTMSQNALKLA 420

Query: 421 QPEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           QPEAVFDIVRD+H+LACQRGPMANIPYMLTSSFT LI
Sbjct: 421 QPEAVFDIVRDIHDLACQRGPMANIPYMLTSSFTTLI 457

BLAST of MS011718 vs. NCBI nr
Match: XP_023538596.1 (monogalactosyldiacylglycerol synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.4 bits (2214), Expect = 5.4e-245
Identity = 414/455 (90.99%), Postives = 436/455 (95.82%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLI 60
           MSVAT +SVTEKVFQRVGGHYQ  S+ R C N  +EEDYDDDG LE+VQIGA+RTKNVLI
Sbjct: 1   MSVATPRSVTEKVFQRVGGHYQ-SSTHRICGNEFKEEDYDDDGTLEMVQIGAERTKNVLI 60

Query: 61  LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 120
           LMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHVQL
Sbjct: 61  LMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHVQL 120

Query: 121 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 180
           WKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKWQG
Sbjct: 121 WKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKWQG 180

Query: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 240
           LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLD +EES+VRVYGLPIRPS
Sbjct: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDDIEESRVRVYGLPIRPS 240

Query: 241 FARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 300
           FARAVLQK +LR+ELEMDP+LPAVLLMGGGEGMGPVK+TAKALAESL+DK++EK IGQIV
Sbjct: 241 FARAVLQKDELREELEMDPELPAVLLMGGGEGMGPVKKTAKALAESLYDKDNEKAIGQIV 300

Query: 301 VICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360
           +ICGRN+ LASSLEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI
Sbjct: 301 IICGRNQTLASSLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360

Query: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 420
           ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA IVA+WFS  TEELHTMSQNALKLAQP
Sbjct: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAAIVAQWFSTKTEELHTMSQNALKLAQP 420

Query: 421 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           EAVFDIVRD+HELACQRGPMANIPYMLTSSFT LI
Sbjct: 421 EAVFDIVRDIHELACQRGPMANIPYMLTSSFTDLI 454

BLAST of MS011718 vs. NCBI nr
Match: XP_022965723.1 (monogalactosyldiacylglycerol synthase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 855.9 bits (2210), Expect = 1.6e-244
Identity = 413/455 (90.77%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLI 60
           MSV T +SVTEKVFQRVGGHYQ  S+ R C N  +EEDYDDDG LE+VQIGA+RTKNVLI
Sbjct: 1   MSVVTPRSVTEKVFQRVGGHYQ-SSTHRICGNEFKEEDYDDDGTLEMVQIGAERTKNVLI 60

Query: 61  LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 120
           LMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHVQL
Sbjct: 61  LMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHVQL 120

Query: 121 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 180
           WKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKWQG
Sbjct: 121 WKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKWQG 180

Query: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 240
           LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLD +EES+VRVYGLPIRPS
Sbjct: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDDIEESRVRVYGLPIRPS 240

Query: 241 FARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 300
           FARAVLQK +LR+ELEMDP+LPAVLLMGGGEGMGPVK+TAKALAESL+DK++EK IGQIV
Sbjct: 241 FARAVLQKDELREELEMDPELPAVLLMGGGEGMGPVKKTAKALAESLYDKDNEKAIGQIV 300

Query: 301 VICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360
           +ICGRN+ LASSLEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI
Sbjct: 301 IICGRNQTLASSLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360

Query: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 420
           ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA IVA+WFS  TEELHTMSQNALKLAQP
Sbjct: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAAIVAQWFSTKTEELHTMSQNALKLAQP 420

Query: 421 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           EAVFDIVRD+HELACQRGPMANIPYMLTSSFT LI
Sbjct: 421 EAVFDIVRDIHELACQRGPMANIPYMLTSSFTDLI 454

BLAST of MS011718 vs. ExPASy Swiss-Prot
Match: O82730 (Monogalactosyldiacylglycerol synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MGD2 PE=1 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 2.6e-210
Identity = 360/465 (77.42%), Postives = 399/465 (85.81%), Query Frame = 0

Query: 5   TRKSVTEKVFQRVGGHYQWGSS----------RRSCRNGCREEDYD----DDGNLELVQI 64
           T  ++ EKV +RV G  +   S          R +  +  R + YD    D+ +LEL+QI
Sbjct: 4   TVMALAEKVLERVYGTSKSAVSVTSGDGEKTHRHTHHHIHRIKSYDDIDEDESSLELIQI 63

Query: 65  GAQRTKNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGS 124
           GA+RTKNVLILMSDTGGGHRASAEAIRDAF+IEFGD+Y++ VKDVWKEYTGWPLNDME S
Sbjct: 64  GAERTKNVLILMSDTGGGHRASAEAIRDAFKIEFGDKYRVIVKDVWKEYTGWPLNDMERS 123

Query: 125 YKFMVKHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQH 184
           YKFMVKHVQLWKVAFHSTSP+WIHS YLAAIAAYYAKEVEAGLMEY+P+IIISVHPLMQH
Sbjct: 124 YKFMVKHVQLWKVAFHSTSPKWIHSCYLAAIAAYYAKEVEAGLMEYKPEIIISVHPLMQH 183

Query: 185 IPLWVLKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQV 244
           IPLWVLKWQ LQK+V+FVTVITDLNTCHPTWFHPGVNRCYCPS  VAKRAL DGL+ESQV
Sbjct: 184 IPLWVLKWQELQKRVLFVTVITDLNTCHPTWFHPGVNRCYCPSQEVAKRALFDGLDESQV 243

Query: 245 RVYGLPIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDK 304
           RV+GLP+RPSFARAVL K  LR+ELEMD DL AVLLMGGGEGMGPVKETAKAL E L+DK
Sbjct: 244 RVFGLPVRPSFARAVLVKDDLRKELEMDQDLRAVLLMGGGEGMGPVKETAKALEEFLYDK 303

Query: 305 NSEKPIGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTI 364
            + KPIGQ+VVICGRNK LAS+LEA +WKIPVKVRGFE QMEKWMGACDCIITKAGPGTI
Sbjct: 304 ENRKPIGQMVVICGRNKKLASALEAIDWKIPVKVRGFETQMEKWMGACDCIITKAGPGTI 363

Query: 365 AEALIRGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTM 424
           AE+LIR LPIILNDYIPGQEKGNVPYVV+NGAGVFTRSPKETARIV EWFS  T+EL   
Sbjct: 364 AESLIRSLPIILNDYIPGQEKGNVPYVVENGAGVFTRSPKETARIVGEWFSTKTDELEQT 423

Query: 425 SQNALKLAQPEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           S NA KLAQPEAVFDIV+D+ EL+ QRGP+A++ Y LTSSF  L+
Sbjct: 424 SDNARKLAQPEAVFDIVKDIDELSEQRGPLASVSYNLTSSFASLV 468

BLAST of MS011718 vs. ExPASy Swiss-Prot
Match: Q0DWQ1 (Probable monogalactosyldiacylglycerol synthase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MGD3 PE=3 SV=2)

HSP 1 Score: 682.6 bits (1760), Expect = 3.1e-195
Identity = 317/412 (76.94%), Postives = 370/412 (89.81%), Query Frame = 0

Query: 40  DDDGNLELVQIGAQRTKNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEY 99
           +++G +ELVQ+GA R KNVLILMSDTGGGHRASA+AI+D F+IEFGD+Y++FVKD+ K++
Sbjct: 84  EEEGTVELVQLGANRAKNVLILMSDTGGGHRASAQAIKDPFRIEFGDDYRVFVKDLCKDH 143

Query: 100 TGWPLNDMEGSYKFMVKHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPD 159
            GWPLN+ME SYKFMVKHVQLWKVAFH+TSPRW+H  YLAA+A++YAK+VEAGL +Y+PD
Sbjct: 144 AGWPLNNMESSYKFMVKHVQLWKVAFHTTSPRWVHCFYLAALASFYAKKVEAGLKKYKPD 203

Query: 160 IIISVHPLMQHIPLWVLKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKR 219
           IIISVHPLMQHIPLWVLKWQGLQ +VVFVTVITDLNTCHPTWFH  VNRCYCPS+ VAKR
Sbjct: 204 IIISVHPLMQHIPLWVLKWQGLQNRVVFVTVITDLNTCHPTWFHADVNRCYCPSEEVAKR 263

Query: 220 ALLDGLEESQVRVYGLPIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKET 279
           A LD L+ SQ+RV+GLPIRPSF RAVL K  LR+ELE+DP+LPAVLLMGGGEGMGPVK+T
Sbjct: 264 AALDDLQPSQIRVFGLPIRPSFCRAVLVKDDLRKELELDPELPAVLLMGGGEGMGPVKKT 323

Query: 280 AKALAESLFDKNSEKPIGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACD 339
           AKAL ESLFDK   KPIGQ++VICGRNK L+SSL+A EWKIP+KVRGFE QMEKWMGACD
Sbjct: 324 AKALGESLFDKELGKPIGQLIVICGRNKTLSSSLQALEWKIPIKVRGFETQMEKWMGACD 383

Query: 340 CIITKAGPGTIAEALIRGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEW 399
           CIITKAGPGTIAEALIRGLPIILND+IPGQE GNVPYVVDNGAGVF++S +ETA++VA W
Sbjct: 384 CIITKAGPGTIAEALIRGLPIILNDFIPGQEVGNVPYVVDNGAGVFSKSSRETAKLVARW 443

Query: 400 FSRNTEELHTMSQNALKLAQPEAVFDIVRDLHELACQRGPMANIPYMLTSSF 452
           F  +++EL  MS+ ALKLAQPEAVFDIVRD+HEL+ ++G ++ I   LTSSF
Sbjct: 444 FGPDSDELKRMSEKALKLAQPEAVFDIVRDIHELSREQGVISQISSSLTSSF 495

BLAST of MS011718 vs. ExPASy Swiss-Prot
Match: Q9SI93 (Monogalactosyldiacylglycerol synthase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MGD3 PE=1 SV=2)

HSP 1 Score: 681.0 bits (1756), Expect = 9.1e-195
Identity = 322/442 (72.85%), Postives = 373/442 (84.39%), Query Frame = 0

Query: 8   SVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNL------------ELVQIGAQRT 67
           S+TEKVF+RV  ++   +      +  R  DY+ +  L            E+ Q+GA+R 
Sbjct: 13  SITEKVFRRVYSNFNISTVEDEYIHRQRSSDYEKESRLRKRGLEDKEEVMEMEQMGAERI 72

Query: 68  KNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMV 127
           K VLILMSDTGGGHRASAEAIRDAF+IEFGD+Y+I +KDVWKEYTGWPLNDME  YKFMV
Sbjct: 73  KTVLILMSDTGGGHRASAEAIRDAFKIEFGDDYRIIIKDVWKEYTGWPLNDMERQYKFMV 132

Query: 128 KHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWV 187
           KHV LW VAFH TSP+WIH  YL+A+AAYYAKE+EAGLMEY+PDIIISVHPLMQHIPLWV
Sbjct: 133 KHVGLWSVAFHGTSPKWIHKSYLSALAAYYAKEIEAGLMEYKPDIIISVHPLMQHIPLWV 192

Query: 188 LKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGL 247
           +KWQGL KKV+FVTVITDLNTCH TWFH GV+RCYCPS  VAKRAL+DGL++SQ+RV+GL
Sbjct: 193 MKWQGLHKKVIFVTVITDLNTCHRTWFHHGVSRCYCPSKEVAKRALVDGLDDSQIRVFGL 252

Query: 248 PIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKP 307
           P+RPSF R +L K +LR+ELE+D +LPAVLLMGGGEGMGPV++TA AL +SL++     P
Sbjct: 253 PVRPSFPRTILNKNELRKELEIDLNLPAVLLMGGGEGMGPVQKTALALGDSLYNSKESNP 312

Query: 308 IGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALI 367
           IGQ++VICGRNK LAS+L + EWKIPVKVRGFE QMEKWMGACDCIITKAGPGTIAEALI
Sbjct: 313 IGQLIVICGRNKVLASTLASHEWKIPVKVRGFETQMEKWMGACDCIITKAGPGTIAEALI 372

Query: 368 RGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNAL 427
            GLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA+IVA+WFS N EEL  MS+NAL
Sbjct: 373 CGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAKIVADWFSNNKEELKKMSENAL 432

Query: 428 KLAQPEAVFDIVRDLHELACQR 438
           KL+QPEAVFDIV+D+H L+ Q+
Sbjct: 433 KLSQPEAVFDIVKDIHHLSQQQ 454

BLAST of MS011718 vs. ExPASy Swiss-Prot
Match: Q6UTZ2 (Probable monogalactosyldiacylglycerol synthase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MGD2 PE=2 SV=2)

HSP 1 Score: 653.7 bits (1685), Expect = 1.6e-186
Identity = 302/397 (76.07%), Postives = 345/397 (86.90%), Query Frame = 0

Query: 56  KNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMV 115
           KNVLILMSDTGGGHRASAEA+RDAF++EFGD YQ+FV+D+ KEY GWPLNDME SYKFM+
Sbjct: 70  KNVLILMSDTGGGHRASAEALRDAFRLEFGDAYQVFVRDLGKEYGGWPLNDMERSYKFMI 129

Query: 116 KHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWV 175
           +HV+LWKVAFH TSPRW+H +YLAA+A +YA EV AG+M Y PDIIISVHPLMQHIPLWV
Sbjct: 130 RHVRLWKVAFHGTSPRWVHGMYLAALAYFYANEVVAGIMRYNPDIIISVHPLMQHIPLWV 189

Query: 176 LKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGL 235
           LKWQ L  KV FVTVITDLNTCHPTWFH GV RCYCPS  VAKRALL GLE SQ+RVYGL
Sbjct: 190 LKWQSLHPKVPFVTVITDLNTCHPTWFHHGVTRCYCPSAEVAKRALLRGLEPSQIRVYGL 249

Query: 236 PIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKP 295
           PIRPSF RAVL K +LR+EL+MDPDLPAVLLMGGGEGMGPV+ETA+AL++ L+D+   +P
Sbjct: 250 PIRPSFCRAVLDKDELRKELDMDPDLPAVLLMGGGEGMGPVEETARALSDELYDRRRRRP 309

Query: 296 IGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALI 355
           +GQIVVICGRN+ L S+L++  W +PVK+RGFEKQMEKWMGACDCIITKAGPGTIAEALI
Sbjct: 310 VGQIVVICGRNQVLRSTLQSSRWNVPVKIRGFEKQMEKWMGACDCIITKAGPGTIAEALI 369

Query: 356 RGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNAL 415
           RGLPIILND+IPGQE GNVPYVVDNGAGVF++ P+E AR VA WF+ +T EL   S NAL
Sbjct: 370 RGLPIILNDFIPGQEVGNVPYVVDNGAGVFSKDPREAARQVARWFTTHTNELRRYSLNAL 429

Query: 416 KLAQPEAVFDIVRDLHELACQRGPMANIPYMLTSSFT 453
           KLAQPEAVFDIV+D+H+L  Q   +  IPY LTSSF+
Sbjct: 430 KLAQPEAVFDIVKDIHKLQQQPATVTRIPYSLTSSFS 466

BLAST of MS011718 vs. ExPASy Swiss-Prot
Match: A2YTP9 (Probable monogalactosyldiacylglycerol synthase 2, chloroplastic OS=Oryza sativa subsp. indica OX=39946 GN=MGD2 PE=3 SV=2)

HSP 1 Score: 652.5 bits (1682), Expect = 3.5e-186
Identity = 302/397 (76.07%), Postives = 344/397 (86.65%), Query Frame = 0

Query: 56  KNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMV 115
           KNVLILMSDTGGGHRASAEA+RDAF++EFGD YQ+FV+D+ KEY GWPLNDME SYKFM+
Sbjct: 70  KNVLILMSDTGGGHRASAEALRDAFRLEFGDAYQVFVRDLGKEYGGWPLNDMERSYKFMI 129

Query: 116 KHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWV 175
           +HV+LWKVAFH TSPRW+H +YLAA+A +YA EV AG+M Y PDIIISVHPLMQHIPLWV
Sbjct: 130 RHVRLWKVAFHGTSPRWVHGMYLAALAYFYANEVVAGIMRYNPDIIISVHPLMQHIPLWV 189

Query: 176 LKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGL 235
           LKWQ L  KV FVTVITDLNTCHPTWFH GV RCYCPS  VAKRALL GLE SQ+RVYGL
Sbjct: 190 LKWQSLHPKVPFVTVITDLNTCHPTWFHHGVTRCYCPSAEVAKRALLRGLEPSQIRVYGL 249

Query: 236 PIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKP 295
           PIRPSF RAVL K +LR+EL+MDPDLPAVLLMGGGEGMGPV+ETA AL++ L+D+   +P
Sbjct: 250 PIRPSFCRAVLDKDELRKELDMDPDLPAVLLMGGGEGMGPVEETATALSDELYDRRRRRP 309

Query: 296 IGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALI 355
           +GQIVVICGRN+ L S+L++  W +PVK+RGFEKQMEKWMGACDCIITKAGPGTIAEALI
Sbjct: 310 VGQIVVICGRNQVLRSTLQSSRWNVPVKIRGFEKQMEKWMGACDCIITKAGPGTIAEALI 369

Query: 356 RGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNAL 415
           RGLPIILND+IPGQE GNVPYVVDNGAGVF++ P+E AR VA WF+ +T EL   S NAL
Sbjct: 370 RGLPIILNDFIPGQEVGNVPYVVDNGAGVFSKDPREAARQVARWFTTHTNELRRYSLNAL 429

Query: 416 KLAQPEAVFDIVRDLHELACQRGPMANIPYMLTSSFT 453
           KLAQPEAVFDIV+D+H+L  Q   +  IPY LTSSF+
Sbjct: 430 KLAQPEAVFDIVKDIHKLQQQPATVTRIPYSLTSSFS 466

BLAST of MS011718 vs. ExPASy TrEMBL
Match: A0A6J1CPM0 (Monogalactosyldiacylglycerol synthase OS=Momordica charantia OX=3673 GN=LOC111013024 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 9.3e-267
Identity = 451/455 (99.12%), Postives = 452/455 (99.34%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLI 60
           MSVATRKSVTEKVFQRVGGHYQ GSSRRSC NGCREEDYDDDGNLELVQIGAQRTKNVLI
Sbjct: 74  MSVATRKSVTEKVFQRVGGHYQCGSSRRSCGNGCREEDYDDDGNLELVQIGAQRTKNVLI 133

Query: 61  LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 120
           LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL
Sbjct: 134 LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 193

Query: 121 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 180
           WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG
Sbjct: 194 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 253

Query: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 240
           LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS
Sbjct: 254 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 313

Query: 241 FARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 300
           FARAVLQKGQLR+ELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV
Sbjct: 314 FARAVLQKGQLREELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 373

Query: 301 VICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360
           VICGRNKALASSLEAGEWKI VKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI
Sbjct: 374 VICGRNKALASSLEAGEWKISVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 433

Query: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 420
           ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP
Sbjct: 434 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 493

Query: 421 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI
Sbjct: 494 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 528

BLAST of MS011718 vs. ExPASy TrEMBL
Match: A0A1S3C6A4 (Monogalactosyldiacylglycerol synthase OS=Cucumis melo OX=3656 GN=LOC103496946 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.5e-248
Identity = 417/457 (91.25%), Postives = 438/457 (95.84%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDY--DDDGNLELVQIGAQRTKNV 60
           MSVAT +S+TEKVFQRVGGHYQW S+ +   N CREEDY  DDDG LE+VQIGA+RTKNV
Sbjct: 1   MSVATPRSITEKVFQRVGGHYQWSSTHKIFGNDCREEDYYDDDDGTLEMVQIGAERTKNV 60

Query: 61  LILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHV 120
           LILMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHV
Sbjct: 61  LILMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHV 120

Query: 121 QLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKW 180
           QLWKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKW
Sbjct: 121 QLWKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKW 180

Query: 181 QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIR 240
           QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLDG+EESQVRVYGLPIR
Sbjct: 181 QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDGIEESQVRVYGLPIR 240

Query: 241 PSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQ 300
           PSFARAVLQK QLR+ELEMD DLPAVLLMGGGEGMGPVK+TAKALAESLFDK +EKPIGQ
Sbjct: 241 PSFARAVLQKDQLREELEMDLDLPAVLLMGGGEGMGPVKKTAKALAESLFDKENEKPIGQ 300

Query: 301 IVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL 360
           +V+ICGRNKALAS+LEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL
Sbjct: 301 LVIICGRNKALASTLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL 360

Query: 361 PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLA 420
           PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA+IVAEWFS  TE+ HTMSQNALKLA
Sbjct: 361 PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAKIVAEWFSTKTEQFHTMSQNALKLA 420

Query: 421 QPEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           QPEAVFDIVRD+H+LACQRGPMANIPYMLTSSFT LI
Sbjct: 421 QPEAVFDIVRDIHDLACQRGPMANIPYMLTSSFTTLI 457

BLAST of MS011718 vs. ExPASy TrEMBL
Match: A0A6J1HPT6 (Monogalactosyldiacylglycerol synthase OS=Cucurbita maxima OX=3661 GN=LOC111465534 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 7.6e-245
Identity = 413/455 (90.77%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLI 60
           MSV T +SVTEKVFQRVGGHYQ  S+ R C N  +EEDYDDDG LE+VQIGA+RTKNVLI
Sbjct: 1   MSVVTPRSVTEKVFQRVGGHYQ-SSTHRICGNEFKEEDYDDDGTLEMVQIGAERTKNVLI 60

Query: 61  LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 120
           LMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHVQL
Sbjct: 61  LMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHVQL 120

Query: 121 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 180
           WKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKWQG
Sbjct: 121 WKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKWQG 180

Query: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 240
           LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLD +EES+VRVYGLPIRPS
Sbjct: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDDIEESRVRVYGLPIRPS 240

Query: 241 FARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 300
           FARAVLQK +LR+ELEMDP+LPAVLLMGGGEGMGPVK+TAKALAESL+DK++EK IGQIV
Sbjct: 241 FARAVLQKDELREELEMDPELPAVLLMGGGEGMGPVKKTAKALAESLYDKDNEKAIGQIV 300

Query: 301 VICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360
           +ICGRN+ LASSLEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI
Sbjct: 301 IICGRNQTLASSLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360

Query: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 420
           ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA IVA+WFS  TEELHTMSQNALKLAQP
Sbjct: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAAIVAQWFSTKTEELHTMSQNALKLAQP 420

Query: 421 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           EAVFDIVRD+HELACQRGPMANIPYMLTSSFT LI
Sbjct: 421 EAVFDIVRDIHELACQRGPMANIPYMLTSSFTDLI 454

BLAST of MS011718 vs. ExPASy TrEMBL
Match: A0A0A0LFA6 (Monogalactosyldiacylglycerol synthase OS=Cucumis sativus OX=3659 GN=Csa_2G008110 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.8e-244
Identity = 412/457 (90.15%), Postives = 433/457 (94.75%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDY--DDDGNLELVQIGAQRTKNV 60
           MSVAT +S+TEKVFQRVGGHYQW S+ +   N  R ED   DDDG LE+VQIGA+RTKNV
Sbjct: 1   MSVATPRSITEKVFQRVGGHYQWSSTHKFFGNDGRVEDCFDDDDGTLEMVQIGAERTKNV 60

Query: 61  LILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHV 120
           LILMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHV
Sbjct: 61  LILMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHV 120

Query: 121 QLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKW 180
           QLWKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKW
Sbjct: 121 QLWKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKW 180

Query: 181 QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIR 240
           QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRALLDG+EESQVRVYGLPIR
Sbjct: 181 QGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRALLDGIEESQVRVYGLPIR 240

Query: 241 PSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQ 300
           PSFARAVLQK QLR ELEMDPDLPAVLLMGGGEGMGPVK+TAKALAESL+DK +EKPIGQ
Sbjct: 241 PSFARAVLQKDQLRGELEMDPDLPAVLLMGGGEGMGPVKKTAKALAESLYDKENEKPIGQ 300

Query: 301 IVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL 360
           +V+ICGRNK LAS+LEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL
Sbjct: 301 LVIICGRNKPLASTLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGL 360

Query: 361 PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLA 420
           PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA+IVAEWFS  TE+ HT SQNALKLA
Sbjct: 361 PIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAKIVAEWFSTKTEQFHTTSQNALKLA 420

Query: 421 QPEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           QPEAVFDIVRD+H+LACQRGPMANIPYMLTSSFT LI
Sbjct: 421 QPEAVFDIVRDIHDLACQRGPMANIPYMLTSSFTTLI 457

BLAST of MS011718 vs. ExPASy TrEMBL
Match: A0A6J1FDA1 (Monogalactosyldiacylglycerol synthase OS=Cucurbita moschata OX=3662 GN=LOC111444335 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 5.5e-243
Identity = 410/455 (90.11%), Postives = 434/455 (95.38%), Query Frame = 0

Query: 1   MSVATRKSVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNLELVQIGAQRTKNVLI 60
           MSVAT +SVTEKVFQRV GHYQ  S+ R C +  +EEDYDDDG LE+VQIGA+RTKNVLI
Sbjct: 1   MSVATPRSVTEKVFQRVRGHYQ-SSTHRICGDEFKEEDYDDDGTLEMVQIGAERTKNVLI 60

Query: 61  LMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQL 120
           LMSDTGGGHRASAEAIRDAF+IEFGDEYQIFVKDVWKEYTGWPLNDME SYKFMVKHVQL
Sbjct: 61  LMSDTGGGHRASAEAIRDAFRIEFGDEYQIFVKDVWKEYTGWPLNDMERSYKFMVKHVQL 120

Query: 121 WKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQG 180
           WKVAFHSTSPRWIHS+YLAAIAAYYAKEVEAGLMEY+PDIIISVHPLMQHIPLWVLKWQG
Sbjct: 121 WKVAFHSTSPRWIHSVYLAAIAAYYAKEVEAGLMEYKPDIIISVHPLMQHIPLWVLKWQG 180

Query: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPS 240
           LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPS+AVAKRA LD +EES+VRVYGLPIRPS
Sbjct: 181 LQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSEAVAKRAFLDDIEESRVRVYGLPIRPS 240

Query: 241 FARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIV 300
           FARAVLQK +LR+ELEMDP+LPAVLLMGGGEGMGPVK+TAKALAESL+DK++EK IGQIV
Sbjct: 241 FARAVLQKDELREELEMDPELPAVLLMGGGEGMGPVKKTAKALAESLYDKDNEKAIGQIV 300

Query: 301 VICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360
           +ICGRN+ LAS+LEA EWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI
Sbjct: 301 IICGRNQTLASTLEAFEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPI 360

Query: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQP 420
           ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA IVA+WFS  TEELHTMSQNALKLAQP
Sbjct: 361 ILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAAIVAQWFSTKTEELHTMSQNALKLAQP 420

Query: 421 EAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           EAVFDIVRD+HELACQRGPMANIPYMLTSSFT LI
Sbjct: 421 EAVFDIVRDIHELACQRGPMANIPYMLTSSFTDLI 454

BLAST of MS011718 vs. TAIR 10
Match: AT5G20410.1 (monogalactosyldiacylglycerol synthase 2 )

HSP 1 Score: 732.6 bits (1890), Expect = 1.9e-211
Identity = 360/465 (77.42%), Postives = 399/465 (85.81%), Query Frame = 0

Query: 5   TRKSVTEKVFQRVGGHYQWGSS----------RRSCRNGCREEDYD----DDGNLELVQI 64
           T  ++ EKV +RV G  +   S          R +  +  R + YD    D+ +LEL+QI
Sbjct: 4   TVMALAEKVLERVYGTSKSAVSVTSGDGEKTHRHTHHHIHRIKSYDDIDEDESSLELIQI 63

Query: 65  GAQRTKNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGS 124
           GA+RTKNVLILMSDTGGGHRASAEAIRDAF+IEFGD+Y++ VKDVWKEYTGWPLNDME S
Sbjct: 64  GAERTKNVLILMSDTGGGHRASAEAIRDAFKIEFGDKYRVIVKDVWKEYTGWPLNDMERS 123

Query: 125 YKFMVKHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQH 184
           YKFMVKHVQLWKVAFHSTSP+WIHS YLAAIAAYYAKEVEAGLMEY+P+IIISVHPLMQH
Sbjct: 124 YKFMVKHVQLWKVAFHSTSPKWIHSCYLAAIAAYYAKEVEAGLMEYKPEIIISVHPLMQH 183

Query: 185 IPLWVLKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQV 244
           IPLWVLKWQ LQK+V+FVTVITDLNTCHPTWFHPGVNRCYCPS  VAKRAL DGL+ESQV
Sbjct: 184 IPLWVLKWQELQKRVLFVTVITDLNTCHPTWFHPGVNRCYCPSQEVAKRALFDGLDESQV 243

Query: 245 RVYGLPIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDK 304
           RV+GLP+RPSFARAVL K  LR+ELEMD DL AVLLMGGGEGMGPVKETAKAL E L+DK
Sbjct: 244 RVFGLPVRPSFARAVLVKDDLRKELEMDQDLRAVLLMGGGEGMGPVKETAKALEEFLYDK 303

Query: 305 NSEKPIGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTI 364
            + KPIGQ+VVICGRNK LAS+LEA +WKIPVKVRGFE QMEKWMGACDCIITKAGPGTI
Sbjct: 304 ENRKPIGQMVVICGRNKKLASALEAIDWKIPVKVRGFETQMEKWMGACDCIITKAGPGTI 363

Query: 365 AEALIRGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTM 424
           AE+LIR LPIILNDYIPGQEKGNVPYVV+NGAGVFTRSPKETARIV EWFS  T+EL   
Sbjct: 364 AESLIRSLPIILNDYIPGQEKGNVPYVVENGAGVFTRSPKETARIVGEWFSTKTDELEQT 423

Query: 425 SQNALKLAQPEAVFDIVRDLHELACQRGPMANIPYMLTSSFTGLI 456
           S NA KLAQPEAVFDIV+D+ EL+ QRGP+A++ Y LTSSF  L+
Sbjct: 424 SDNARKLAQPEAVFDIVKDIDELSEQRGPLASVSYNLTSSFASLV 468

BLAST of MS011718 vs. TAIR 10
Match: AT2G11810.1 (monogalactosyldiacylglycerol synthase type C )

HSP 1 Score: 681.0 bits (1756), Expect = 6.5e-196
Identity = 322/442 (72.85%), Postives = 373/442 (84.39%), Query Frame = 0

Query: 8   SVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNL------------ELVQIGAQRT 67
           S+TEKVF+RV  ++   +      +  R  DY+ +  L            E+ Q+GA+R 
Sbjct: 13  SITEKVFRRVYSNFNISTVEDEYIHRQRSSDYEKESRLRKRGLEDKEEVMEMEQMGAERI 72

Query: 68  KNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMV 127
           K VLILMSDTGGGHRASAEAIRDAF+IEFGD+Y+I +KDVWKEYTGWPLNDME  YKFMV
Sbjct: 73  KTVLILMSDTGGGHRASAEAIRDAFKIEFGDDYRIIIKDVWKEYTGWPLNDMERQYKFMV 132

Query: 128 KHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWV 187
           KHV LW VAFH TSP+WIH  YL+A+AAYYAKE+EAGLMEY+PDIIISVHPLMQHIPLWV
Sbjct: 133 KHVGLWSVAFHGTSPKWIHKSYLSALAAYYAKEIEAGLMEYKPDIIISVHPLMQHIPLWV 192

Query: 188 LKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGL 247
           +KWQGL KKV+FVTVITDLNTCH TWFH GV+RCYCPS  VAKRAL+DGL++SQ+RV+GL
Sbjct: 193 MKWQGLHKKVIFVTVITDLNTCHRTWFHHGVSRCYCPSKEVAKRALVDGLDDSQIRVFGL 252

Query: 248 PIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKP 307
           P+RPSF R +L K +LR+ELE+D +LPAVLLMGGGEGMGPV++TA AL +SL++     P
Sbjct: 253 PVRPSFPRTILNKNELRKELEIDLNLPAVLLMGGGEGMGPVQKTALALGDSLYNSKESNP 312

Query: 308 IGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALI 367
           IGQ++VICGRNK LAS+L + EWKIPVKVRGFE QMEKWMGACDCIITKAGPGTIAEALI
Sbjct: 313 IGQLIVICGRNKVLASTLASHEWKIPVKVRGFETQMEKWMGACDCIITKAGPGTIAEALI 372

Query: 368 RGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETARIVAEWFSRNTEELHTMSQNAL 427
            GLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETA+IVA+WFS N EEL  MS+NAL
Sbjct: 373 CGLPIILNDYIPGQEKGNVPYVVDNGAGVFTRSPKETAKIVADWFSNNKEELKKMSENAL 432

Query: 428 KLAQPEAVFDIVRDLHELACQR 438
           KL+QPEAVFDIV+D+H L+ Q+
Sbjct: 433 KLSQPEAVFDIVKDIHHLSQQQ 454

BLAST of MS011718 vs. TAIR 10
Match: AT4G31780.2 (monogalactosyl diacylglycerol synthase 1 )

HSP 1 Score: 540.4 bits (1391), Expect = 1.4e-153
Identity = 259/423 (61.23%), Postives = 323/423 (76.36%), Query Frame = 0

Query: 24  GSSRRSCRNGCREEDYD---DDGNLELVQIGAQRTKNVLILMSDTGGGHRASAEAIRDAF 83
           G    S  NG RE        +  L L  + A R K VLILMSDTGGGHRASAEAIR AF
Sbjct: 107 GVGLSSDENGIRENGTGGVLGEEGLPLNGVEADRPKKVLILMSDTGGGHRASAEAIRAAF 166

Query: 84  QIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQLWKVAFHSTSPRWIHSLYLAA 143
             EFGDEYQ+F+ D+W ++T WP N +  SY F+VKH  LWK+ ++ TSPR +H    AA
Sbjct: 167 NQEFGDEYQVFITDLWTDHTPWPFNQLPRSYNFLVKHGTLWKMTYYGTSPRIVHQSNFAA 226

Query: 144 IAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQGLQKKVVFVTVITDLNTCHPT 203
            + + A+E+  GLM+Y+PDIIISVHPLMQH+PL VL+ +GL KK+VF TVITDL+TCHPT
Sbjct: 227 TSTFIAREIAQGLMKYQPDIIISVHPLMQHVPLRVLRSKGLLKKIVFTTVITDLSTCHPT 286

Query: 204 WFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPSFARAVLQKGQLRQELEMDPD 263
           WFH  V RCYCPS  VAKRA   GLE SQ++VYGLP+RPSF + V  K +LR+EL MD +
Sbjct: 287 WFHKLVTRCYCPSTEVAKRAQKAGLETSQIKVYGLPVRPSFVKPVRPKVELRRELGMDEN 346

Query: 264 LPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIVVICGRNKALASSLEAGEWKI 323
           LPAVLLMGGGEGMGP++ TA+ALA++L+DKN  + +GQ+++ICGRNK L S L + +WKI
Sbjct: 347 LPAVLLMGGGEGMGPIEATARALADALYDKNLGEAVGQVLIICGRNKKLQSKLSSLDWKI 406

Query: 324 PVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPIILNDYIPGQEKGNVPYVVDN 383
           PV+V+GF  +ME+ MGACDCIITKAGPGTIAEA+IRGLPIILN YI GQE GNVPYVV+N
Sbjct: 407 PVQVKGFITKMEECMGACDCIITKAGPGTIAEAMIRGLPIILNGYIAGQEAGNVPYVVEN 466

Query: 384 GAGVFTRSPKETARIVAEWFSRNTEELHTMSQNALKLAQPEAVFDIVRDLHELACQRGPM 443
           G G F++SPKE ++IVA+WF   ++EL  MSQNAL+LA+PEAVF IV D+HEL  ++  +
Sbjct: 467 GCGKFSKSPKEISKIVADWFGPASKELEIMSQNALRLAKPEAVFKIVHDMHELVRKKNSL 526

BLAST of MS011718 vs. TAIR 10
Match: AT2G11810.2 (monogalactosyldiacylglycerol synthase type C )

HSP 1 Score: 522.3 bits (1344), Expect = 3.9e-148
Identity = 244/349 (69.91%), Postives = 287/349 (82.23%), Query Frame = 0

Query: 8   SVTEKVFQRVGGHYQWGSSRRSCRNGCREEDYDDDGNL------------ELVQIGAQRT 67
           S+TEKVF+RV  ++   +      +  R  DY+ +  L            E+ Q+GA+R 
Sbjct: 13  SITEKVFRRVYSNFNISTVEDEYIHRQRSSDYEKESRLRKRGLEDKEEVMEMEQMGAERI 72

Query: 68  KNVLILMSDTGGGHRASAEAIRDAFQIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMV 127
           K VLILMSDTGGGHRASAEAIRDAF+IEFGD+Y+I +KDVWKEYTGWPLNDME  YKFMV
Sbjct: 73  KTVLILMSDTGGGHRASAEAIRDAFKIEFGDDYRIIIKDVWKEYTGWPLNDMERQYKFMV 132

Query: 128 KHVQLWKVAFHSTSPRWIHSLYLAAIAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWV 187
           KHV LW VAFH TSP+WIH  YL+A+AAYYAKE+EAGLMEY+PDIIISVHPLMQHIPLWV
Sbjct: 133 KHVGLWSVAFHGTSPKWIHKSYLSALAAYYAKEIEAGLMEYKPDIIISVHPLMQHIPLWV 192

Query: 188 LKWQGLQKKVVFVTVITDLNTCHPTWFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGL 247
           +KWQGL KKV+FVTVITDLNTCH TWFH GV+RCYCPS  VAKRAL+DGL++SQ+RV+GL
Sbjct: 193 MKWQGLHKKVIFVTVITDLNTCHRTWFHHGVSRCYCPSKEVAKRALVDGLDDSQIRVFGL 252

Query: 248 PIRPSFARAVLQKGQLRQELEMDPDLPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKP 307
           P+RPSF R +L K +LR+ELE+D +LPAVLLMGGGEGMGPV++TA AL +SL++     P
Sbjct: 253 PVRPSFPRTILNKNELRKELEIDLNLPAVLLMGGGEGMGPVQKTALALGDSLYNSKESNP 312

Query: 308 IGQIVVICGRNKALASSLEAGEWKIPVKVRGFEKQMEKWMGACDCIITK 345
           IGQ++VICGRNK LAS+L + EWKIPVKVRGFE QMEKWMGACDCIITK
Sbjct: 313 IGQLIVICGRNKVLASTLASHEWKIPVKVRGFETQMEKWMGACDCIITK 361

BLAST of MS011718 vs. TAIR 10
Match: AT4G31780.1 (monogalactosyl diacylglycerol synthase 1 )

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-126
Identity = 217/349 (62.18%), Postives = 266/349 (76.22%), Query Frame = 0

Query: 24  GSSRRSCRNGCREEDYD---DDGNLELVQIGAQRTKNVLILMSDTGGGHRASAEAIRDAF 83
           G    S  NG RE        +  L L  + A R K VLILMSDTGGGHRASAEAIR AF
Sbjct: 107 GVGLSSDENGIRENGTGGVLGEEGLPLNGVEADRPKKVLILMSDTGGGHRASAEAIRAAF 166

Query: 84  QIEFGDEYQIFVKDVWKEYTGWPLNDMEGSYKFMVKHVQLWKVAFHSTSPRWIHSLYLAA 143
             EFGDEYQ+F+ D+W ++T WP N +  SY F+VKH  LWK+ ++ TSPR +H    AA
Sbjct: 167 NQEFGDEYQVFITDLWTDHTPWPFNQLPRSYNFLVKHGTLWKMTYYGTSPRIVHQSNFAA 226

Query: 144 IAAYYAKEVEAGLMEYRPDIIISVHPLMQHIPLWVLKWQGLQKKVVFVTVITDLNTCHPT 203
            + + A+E+  GLM+Y+PDIIISVHPLMQH+PL VL+ +GL KK+VF TVITDL+TCHPT
Sbjct: 227 TSTFIAREIAQGLMKYQPDIIISVHPLMQHVPLRVLRSKGLLKKIVFTTVITDLSTCHPT 286

Query: 204 WFHPGVNRCYCPSDAVAKRALLDGLEESQVRVYGLPIRPSFARAVLQKGQLRQELEMDPD 263
           WFH  V RCYCPS  VAKRA   GLE SQ++VYGLP+RPSF + V  K +LR+EL MD +
Sbjct: 287 WFHKLVTRCYCPSTEVAKRAQKAGLETSQIKVYGLPVRPSFVKPVRPKVELRRELGMDEN 346

Query: 264 LPAVLLMGGGEGMGPVKETAKALAESLFDKNSEKPIGQIVVICGRNKALASSLEAGEWKI 323
           LPAVLLMGGGEGMGP++ TA+ALA++L+DKN  + +GQ+++ICGRNK L S L + +WKI
Sbjct: 347 LPAVLLMGGGEGMGPIEATARALADALYDKNLGEAVGQVLIICGRNKKLQSKLSSLDWKI 406

Query: 324 PVKVRGFEKQMEKWMGACDCIITKAGPGTIAEALIRGLPIILNDYIPGQ 370
           PV+V+GF  +ME+ MGACDCIITKAGPGTIAEA+IRGLPIILN YI GQ
Sbjct: 407 PVQVKGFITKMEECMGACDCIITKAGPGTIAEAMIRGLPIILNGYIAGQ 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143037.11.9e-26699.12monogalactosyldiacylglycerol synthase 2, chloroplastic [Momordica charantia][more]
XP_038889391.11.6e-24991.89monogalactosyldiacylglycerol synthase 2, chloroplastic [Benincasa hispida][more]
XP_008457220.15.2e-24891.25PREDICTED: monogalactosyldiacylglycerol synthase 2, chloroplastic [Cucumis melo][more]
XP_023538596.15.4e-24590.99monogalactosyldiacylglycerol synthase 2, chloroplastic-like [Cucurbita pepo subs... [more]
XP_022965723.11.6e-24490.77monogalactosyldiacylglycerol synthase 2, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O827302.6e-21077.42Monogalactosyldiacylglycerol synthase 2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q0DWQ13.1e-19576.94Probable monogalactosyldiacylglycerol synthase 3, chloroplastic OS=Oryza sativa ... [more]
Q9SI939.1e-19572.85Monogalactosyldiacylglycerol synthase 3, chloroplastic OS=Arabidopsis thaliana O... [more]
Q6UTZ21.6e-18676.07Probable monogalactosyldiacylglycerol synthase 2, chloroplastic OS=Oryza sativa ... [more]
A2YTP93.5e-18676.07Probable monogalactosyldiacylglycerol synthase 2, chloroplastic OS=Oryza sativa ... [more]
Match NameE-valueIdentityDescription
A0A6J1CPM09.3e-26799.12Monogalactosyldiacylglycerol synthase OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A1S3C6A42.5e-24891.25Monogalactosyldiacylglycerol synthase OS=Cucumis melo OX=3656 GN=LOC103496946 PE... [more]
A0A6J1HPT67.6e-24590.77Monogalactosyldiacylglycerol synthase OS=Cucurbita maxima OX=3661 GN=LOC11146553... [more]
A0A0A0LFA63.8e-24490.15Monogalactosyldiacylglycerol synthase OS=Cucumis sativus OX=3659 GN=Csa_2G008110... [more]
A0A6J1FDA15.5e-24390.11Monogalactosyldiacylglycerol synthase OS=Cucurbita moschata OX=3662 GN=LOC111444... [more]
Match NameE-valueIdentityDescription
AT5G20410.11.9e-21177.42monogalactosyldiacylglycerol synthase 2 [more]
AT2G11810.16.5e-19672.85monogalactosyldiacylglycerol synthase type C [more]
AT4G31780.21.4e-15361.23monogalactosyl diacylglycerol synthase 1 [more]
AT2G11810.23.9e-14869.91monogalactosyldiacylglycerol synthase type C [more]
AT4G31780.11.1e-12662.18monogalactosyl diacylglycerol synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007235Glycosyl transferase, family 28, C-terminalPFAMPF04101Glyco_tran_28_Ccoord: 264..389
e-value: 2.6E-10
score: 40.5
IPR009695Diacylglycerol glucosyltransferase, N-terminalPFAMPF06925MGDG_synthcoord: 69..237
e-value: 7.5E-61
score: 205.1
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 246..418
e-value: 1.7E-30
score: 108.0
NoneNo IPR availablePANTHERPTHR43025MONOGALACTOSYLDIACYLGLYCEROL SYNTHASEcoord: 3..453
NoneNo IPR availablePANTHERPTHR43025:SF1MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTICcoord: 3..453
NoneNo IPR availableCDDcd17507GT28_Beta-DGS-likecoord: 58..433
e-value: 7.11731E-139
score: 400.926
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 58..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011718.1MS011718.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009247 glycolipid biosynthetic process
molecular_function GO:0016758 hexosyltransferase activity