MS011477 (gene) Bitter gourd (TR) v1

BLAST of MS011477 vs. NCBI nr
Match: XP_038881674.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 703/761 (92.38%), Postives = 734/761 (96.45%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAE 91
           MA PLWIF++ CFF    SMAAA+KKTYIVH+AKY+MP++F+HHLHWYDSSL+SVSDTAE
Sbjct: 1   MANPLWIFLLLCFF-SVPSMAAAQKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60

Query: 92  MIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLYP 151
           MIYAYNNVVHGFSTRLT EEA+RLES+P ILAVVPEMRYELHTTRTPQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120

Query: 152 ESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARY 211
           ESNS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 212 FSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARA 271
           FSKGYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240

Query: 272 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG 331
           RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG 300

Query: 332 ILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQ 391
           IL+SCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK L 
Sbjct: 301 ILVSCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKALP 360

Query: 392 ATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 451
            TLLPFIYA NASN+ NGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 452 VLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 511
           VLANTA NGEELVADSHLLPATAVGQKSGDI+RKYLISEPNPTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIMRKYLISEPNPTVTILFEGTKLGIEPSPV 480

Query: 512 VAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSC 571
           VAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL IDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLPIDDRRVDFNIISGTSMSC 540

Query: 572 PHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVD 631
           PHVSGLAAL+KGAHPDWSPAAIRSALMTTAYTAY++GQK+QD+ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYRNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 632 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFAV 691
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLARRDFTCD+ KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660

Query: 692 VFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEKK 751
           VF+G L G G GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720

Query: 752 SYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           SYTVTFTT +GSAAPPS EGFGRIEWSDGK VVGSPIAFSW
Sbjct: 721 SYTVTFTTVTGSAAPPSAEGFGRIEWSDGKQVVGSPIAFSW 760

BLAST of MS011477 vs. NCBI nr
Match: XP_023543760.1 (subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 691/762 (90.68%), Postives = 735/762 (96.46%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGR-LSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTA 91
           M KP+W+F++FCFF G  ++ AAA+KKTY+VH+AKY+MP  F+HHLHWYD+SLKSVSDTA
Sbjct: 1   MEKPVWVFLLFCFFSGPFMAAAAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDTA 60

Query: 92  EMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLY 151
           +MIYAY  VVHGFSTRLT EEA+RLE++P +LAVVPEMRYELHTTRTPQFLGLDKNADLY
Sbjct: 61  DMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADLY 120

Query: 152 PESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGAR 211
           PES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGAR
Sbjct: 121 PESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180

Query: 212 YFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAAR 271
           +FS+GYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAAR
Sbjct: 181 FFSRGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240

Query: 272 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEK 331
           ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAMEK
Sbjct: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300

Query: 332 GILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPL 391
           GILISCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK L
Sbjct: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKSL 360

Query: 392 QATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 451
             TLLPFIYA NASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIG
Sbjct: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIG 420

Query: 452 MVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSP 511
           MVLANTA NGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPSP
Sbjct: 421 MVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPSP 480

Query: 512 VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMS 571
           VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGL ID+RRVDFNIISGTSMS
Sbjct: 481 VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSMS 540

Query: 572 CPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHV 631
           CPHVSGLAAL+KGAH DWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGHV
Sbjct: 541 CPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 600

Query: 632 DPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFA 691
           DPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCD+GKKYSVN+LNYPSFA
Sbjct: 601 DPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSFA 660

Query: 692 VVFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK 751
           VVF+G L G   GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK
Sbjct: 661 VVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK 720

Query: 752 KSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           KSYTVTFTTA+GS+APPS EGFGRIEW+DGKHVVGSPIAFSW
Sbjct: 721 KSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSW 762

BLAST of MS011477 vs. NCBI nr
Match: KAG6604148.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1401.3 bits (3626), Expect = 0.0e+00
Identity = 693/763 (90.83%), Postives = 733/763 (96.07%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMA--AAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 91
           M KP+WIF++FCFF G    A  AA+KKTY+VH+AKY+MP  F+HHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 92  AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADL 151
           A+MIYAY  VVHGFSTRLT EEA+RLE++P +LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 152 YPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGA 211
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 212 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAA 271
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 272 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAME 331
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 332 KGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 391
           KGILISCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 392 LQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 451
           L  TLLPFIYA NASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 452 GMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 511
           GMVLANTA NGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 512 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSM 571
           PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 572 SCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGH 631
           SCPHVSGLAAL+KGAH DWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 632 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSF 691
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 692 AVVFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 751
           AVVF+G L G   GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 752 KKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           KKSYTVTFTTA+GS+APPS EGFGRIEW+DGKHVVGSPIAFSW
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSW 763

BLAST of MS011477 vs. NCBI nr
Match: KAG7034309.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 692/763 (90.69%), Postives = 733/763 (96.07%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMA--AAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 91
           M KP+WIF++FCFF G    A  AA+KKTY+VH+AKY+MP  F+HHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 92  AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADL 151
           A+MIYAY  VVHGFSTRLT EEA+RLE++P +LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 152 YPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGA 211
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 212 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAA 271
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 272 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAME 331
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+G+FAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGSFAAME 300

Query: 332 KGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 391
           KGILISCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 392 LQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 451
           L  TLLPFIYA NASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 452 GMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 511
           GMVLANTA NGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 512 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSM 571
           PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 572 SCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGH 631
           SCPHVSGLAAL+KGAH DWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 632 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSF 691
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 692 AVVFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 751
           AVVF+G L G   GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 752 KKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           KKSYTVTFTTA+GS+APPS EGFGRIEW+DGKHVVGSPIAFSW
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSW 763

BLAST of MS011477 vs. NCBI nr
Match: XP_022977447.1 (subtilisin-like protease SBT1.7 [Cucurbita maxima])

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 693/763 (90.83%), Postives = 734/763 (96.20%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYG--RLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 91
           M KP+ IF++FCFF G    + AAA+KKTY+VH+AKY+MP  F+HHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVRIFLLFCFFSGPFMAAAAAAQKKTYVVHMAKYQMPLTFEHHLHWYDASLKSVSDT 60

Query: 92  AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADL 151
           A+MIYAY  VVHGFSTRLT EEA+RLE++P +LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAKRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 152 YPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGA 211
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 212 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAA 271
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 272 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAME 331
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 332 KGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 391
           KGILISCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGKP
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKP 360

Query: 392 LQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 451
           L  TLLPFIYA NASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LSGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 452 GMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 511
           GMVLANTA NGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 512 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSM 571
           PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDERRVDFNIISGTSM 540

Query: 572 SCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGH 631
           SCPHVSGLAAL+KGAH DWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 632 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSF 691
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 692 AVVFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 751
           AVVF+G L G   GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 752 KKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           KKSYTVTFTTA+GS+ PPS EGFGRIEW+DGKHVVGSPIAFSW
Sbjct: 721 KKSYTVTFTTATGSSVPPSAEGFGRIEWTDGKHVVGSPIAFSW 763

BLAST of MS011477 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 543/755 (71.92%), Postives = 637/755 (84.37%), Query Frame = 0

Query: 39  FVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAEMIYAYNN 98
           F++ C  +  +S +++++ TYIVH+AK +MP +FD H +WYDSSL+S+SD+AE++Y Y N
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYEN 71

Query: 99  VVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKN-ADLYPESNSAS 158
            +HGFSTRLT EEA  L ++P +++V+PE RYELHTTRTP FLGLD++ ADL+PE+ S S
Sbjct: 72  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 131

Query: 159 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARYFSKGYE 218
           +V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNFTA+ CNRKLIGAR+F++GYE
Sbjct: 132 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 191

Query: 219 ATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARARVAAYK 278
           +T+GPIDESKESRSPRDDDGHGTHTS+TAAGSVVE ASL GYASGTARGMA RARVA YK
Sbjct: 192 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 251

Query: 279 VCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGILISCS 338
           VCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+GIL+SCS
Sbjct: 252 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 311

Query: 339 AGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQATLLPF 398
           AGNAGPS  SLSN APWITTVGAGTLDRDFPA   LG+GKNF+GVSL++G+ L   LLPF
Sbjct: 312 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 371

Query: 399 IYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTA 458
           IYAGNASNA+NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM+LANTA
Sbjct: 372 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 431

Query: 459 TNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSS 518
            NGEELVAD+HLLPAT VG+K+GDIIR Y+ ++PNPT +I   GT +G++PSPVVAAFSS
Sbjct: 432 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSS 491

Query: 519 RGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSCPHVSGL 578
           RGPNSITP +LKPD+IAPGVNILA W+ A GP+GLA D RRV+FNIISGTSMSCPHVSGL
Sbjct: 492 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 551

Query: 579 AALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVDPVSALN 638
           AAL+K  HP+WSPAAIRSALMTTAY  YK G+ L D+ATGKPSTPFDHGAGHV P +A N
Sbjct: 552 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATN 611

Query: 639 PGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFAVVFEGAL 698
           PGL+YDLT +DYL FLCALNYTS QI S++RR++TCD  K YSV DLNYPSFAV  +G  
Sbjct: 612 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV- 671

Query: 699 SGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEKKSYTVTF 758
                     K+TRT+T+VG  GTY V ++SET  VKISVEP  L+F +ANEKKSYTVTF
Sbjct: 672 -------GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF 731

Query: 759 TTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           T    S+ P  +  FG IEWSDGKHVVGSP+A SW
Sbjct: 732 TV--DSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MS011477 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 7.0e-235
Identity = 422/759 (55.60%), Postives = 545/759 (71.81%), Query Frame = 0

Query: 38  IFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAEMIYAYN 97
           I +I  F +  L +    KKTYI+ V     P++F  H  WY S L S S    ++Y Y 
Sbjct: 11  ITIITTFLF--LLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYT 70

Query: 98  NVVHGFSTRLTPEEA-RRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLYPESNSA 157
              HGFS  L   EA   L S   IL +  +  Y LHTTRTP+FLGL+    ++   +S+
Sbjct: 71  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 130

Query: 158 SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARYFSKGY 217
           + VIIGVLDTGVWPES+SFDDT +  +PS WKGECESG++F +  CN+KLIGAR FSKG+
Sbjct: 131 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 190

Query: 218 E-ATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARARVAA 277
           + A+ G     +ES SPRD DGHGTHTSTTAAGS V NAS  GYA+GTARGMA RARVA 
Sbjct: 191 QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVAT 250

Query: 278 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGILIS 337
           YKVCW+ GCF SDILAA+++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +S
Sbjct: 251 YKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVS 310

Query: 338 CSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQATLL 397
           CSAGN+GP+  S++N APW+ TVGAGTLDRDFPA+ +LG+GK  +GVSLY G  +    L
Sbjct: 311 CSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPL 370

Query: 398 PFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLAN 457
             +Y  N  N+S+ NLC+ G+L    V GKIV CDRGVN RV+KGAVV+ AGG+GM++AN
Sbjct: 371 ELVY--NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMAN 430

Query: 458 TATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAF 517
           TA +GEELVADSHLLPA AVG+K+GD++R+Y+ S+  PT  ++F+GT L ++PSPVVAAF
Sbjct: 431 TAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAF 490

Query: 518 SSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSCPHVS 577
           SSRGPN++TP++LKPD+I PGVNILAGWS A+GP+GL  D RR  FNI+SGTSMSCPH+S
Sbjct: 491 SSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHIS 550

Query: 578 GLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVDPVSA 637
           GLA L+K AHP+WSP+AI+SALMTTAY    +   L D A    S P+ HG+GHVDP  A
Sbjct: 551 GLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKA 610

Query: 638 LNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYS-VNDLNYPSFAVVFE 697
           L+PGLVYD++ ++Y+ FLC+L+YT   I ++ +R  + +  KK+S    LNYPSF+V+F 
Sbjct: 611 LSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRP-SVNCSKKFSDPGQLNYPSFSVLF- 670

Query: 698 GALSGQGGGSTVVKHTRTLTNVGSPGT-YKVSISSETKLVKISVEPESLSFTKANEKKSY 757
                  GG  VV++TR +TNVG+  + YKV+++     V ISV+P  LSF    EKK Y
Sbjct: 671 -------GGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRY 730

Query: 758 TVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           TVTF +  G +     E FG I WS+ +H V SP+AFSW
Sbjct: 731 TVTFVSKKGVSMTNKAE-FGSITWSNPQHEVRSPVAFSW 751

BLAST of MS011477 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 6.6e-225
Identity = 399/780 (51.15%), Postives = 529/780 (67.82%), Query Frame = 0

Query: 32  MAKPLWIFVI---FCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVS- 91
           + KP    ++     F     +   + KKTY++H+ K  MP  + +HL WY S + SV+ 
Sbjct: 7   LQKPFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQ 66

Query: 92  --------DTAEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQ 151
                   +   ++Y Y    HG + +LT EEA RLE    ++AV+PE RYELHTTR+P 
Sbjct: 67  HKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPT 126

Query: 152 FLGLDKNAD--LYPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF 211
           FLGL++     ++ E  +  +V++GVLDTG+WPES+SF+DTG+ PVP++W+G CE+G  F
Sbjct: 127 FLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRF 186

Query: 212 TAANCNRKLIGARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLF 271
              NCNRK++GAR F +GYEA  G IDE  E +SPRD DGHGTHT+ T AGS V+ A+LF
Sbjct: 187 LKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLF 246

Query: 272 GYASGTARGMAARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYK 331
           G+A GTARGMA +ARVAAYKVCW GGCFSSDIL+A+++AV D V VLS+SLGGG+S Y +
Sbjct: 247 GFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSR 306

Query: 332 DSVATGAFAAMEKGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGK 391
           DS++   F AME G+ +SCSAGN GP P SL+N +PWITTVGA T+DRDFPA V +G  +
Sbjct: 307 DSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMR 366

Query: 392 NFSGVSLYRGKPL--QATLLPFIYAG-NASNASNGNLCMTGTLIPEKVAGKIVFCDRGVN 451
            F GVSLY+G+ +  +    P +Y G NAS+    + C+ G L    VAGKIV CDRGV 
Sbjct: 367 TFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVT 426

Query: 452 PRVQKGAVVKAAGGIGMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPT 511
           PRVQKG VVK AGGIGMVL NTATNGEELVADSH+LPA AVG+K G +I++Y ++    T
Sbjct: 427 PRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKAT 486

Query: 512 VTILFEGTKLGIEPSPVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAI 571
            ++   GT++GI+PSPVVAAFSSRGPN ++ ++LKPD++APGVNILA W+  + PS L+ 
Sbjct: 487 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 546

Query: 572 DDRRVDFNIISGTSMSCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDL 631
           D RRV FNI+SGTSMSCPHVSG+AAL+K  HPDWSPAAI+SALMTTAY      + L D 
Sbjct: 547 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 606

Query: 632 ATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARR-DFTC 691
           +   PS+P+DHGAGH+DP+ A +PGLVYD+   +Y  FLC  + + SQ+    +  + TC
Sbjct: 607 SGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC 666

Query: 692 DAGKKYSVNDLNYPSFAVVFEGALSGQGGGSTVVKHTRTLTNVGSP-GTYKVSISSETKL 751
                 +  +LNYP+ + +F      +      +   RT+TNVG    +YKVS+ S  K 
Sbjct: 667 KHTLAKNPGNLNYPAISALFP-----ENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKG 726

Query: 752 VKISVEPESLSFTKANEKKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
             ++V+P++L+FT  ++K SYTVTF T      P     FG + W    H V SP+  +W
Sbjct: 727 ASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE----FGGLVWKSTTHKVRSPVIITW 776

BLAST of MS011477 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 1.1e-224
Identity = 411/779 (52.76%), Postives = 534/779 (68.55%), Query Frame = 0

Query: 30  IAMAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 89
           ++++   ++F +   F+   S ++   ++YIVHV +   P  F  H +W+ S L+S+  +
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSS 63

Query: 90  ---AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKN 149
              A ++Y+Y+  VHGFS RL+P +   L   P +++V+P+   E+HTT TP FLG  +N
Sbjct: 64  PQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN 123

Query: 150 ADLYPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKL 209
           + L+  SN   +VI+GVLDTG+WPE  SF D+GLGP+PS+WKGECE G +F A++CNRKL
Sbjct: 124 SGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKL 183

Query: 210 IGARYFSKGYEATLGPIDE--SKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTA 269
           IGAR F +GY        +  +KESRSPRD +GHGTHT++TAAGSVV NASL+ YA GTA
Sbjct: 184 IGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTA 243

Query: 270 RGMAARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGISDYYKDSVAT 329
            GMA++AR+AAYK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A 
Sbjct: 244 TGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAI 303

Query: 330 GAFAAMEKGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGV 389
           GAF A   GI++SCSAGN+GP+P + +N APWI TVGA T+DR+F A    GDGK F+G 
Sbjct: 304 GAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGT 363

Query: 390 SLYRGKPLQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAV 449
           SLY G+ L  + L  +Y+G+  +     LC  G L    V GKIV CDRG N RV+KG+ 
Sbjct: 364 SLYAGESLPDSQLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 423

Query: 450 VKAAGGIGMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGT 509
           VK AGG GM+LANTA +GEEL ADSHL+PAT VG K+GD IR Y+ +  +PT  I F GT
Sbjct: 424 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 483

Query: 510 KLG-IEPSPVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDF 569
            +G   PSP VAAFSSRGPN +TP +LKPD+IAPGVNILAGW+  VGP+ L ID RRV F
Sbjct: 484 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 543

Query: 570 NIISGTSMSCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPST 629
           NIISGTSMSCPHVSGLAAL++ AHPDWSPAAI+SAL+TTAY    SG+ ++DLATGK S 
Sbjct: 544 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 603

Query: 630 PFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDF---TCDAGKK 689
            F HGAGHVDP  ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C+  K 
Sbjct: 604 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 663

Query: 690 YSVNDLNYPSFAVVFEGALSGQGGGSTVVKHTRTLTNVGS--PGTYKVSISSETKLVKIS 749
            +  DLNYPSF+VVF  A +G+     VVK+ R + NVGS     Y+V + S    V+I 
Sbjct: 664 RTAGDLNYPSFSVVF--ASTGE-----VVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEID 723

Query: 750 VEPESLSFTKANEKKSYTVTFTT---ASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           V P  L+F+K      Y VTF +     G  + P  E FG IEW+DG+HVV SP+A  W
Sbjct: 724 VSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHE-FGSIEWTDGEHVVKSPVAVQW 769

BLAST of MS011477 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 2.9e-220
Identity = 399/773 (51.62%), Postives = 525/773 (67.92%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSV-SDTA 91
           MA   + F +        S +++   TYIVHV     P  F  H HWY SSL S+ S   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60

Query: 92  EMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGL--DKNAD 151
            +I+ Y+ V HGFS RLT ++A +L   P +++V+PE    LHTTR+P+FLGL     A 
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120

Query: 152 LYPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIG 211
           L  ES+  S+++IGV+DTGVWPE  SFDD GLGPVP  WKG+C +  +F  + CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180

Query: 212 ARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMA 271
           AR+F  GYEAT G ++E+ E RSPRD DGHGTHT++ +AG  V  AS  GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240

Query: 272 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAM 331
            +AR+AAYKVCW  GC+ SDILAA + AV D V+V+S+S+GG +  YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300

Query: 332 EKGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGK 391
           ++GI +S SAGN GP   +++N APW+TTVGAGT+DRDFPA V LG+GK  SGVS+Y G 
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360

Query: 392 PLQ-ATLLPFIYAGN--ASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 451
            L    + P +Y G+    +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+ 
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420

Query: 452 AGGIGMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLI------SEPNPTVTILF 511
            GG+GM++AN   +GE LVAD H+LPAT+VG   GD IR+Y+       S  +PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480

Query: 512 EGTKLGIEPSPVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRV 571
           +GT+LGI P+PVVA+FS+RGPN  TP++LKPD+IAPG+NILA W   +GPSG+  D+RR 
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540

Query: 572 DFNIISGTSMSCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKP 631
           +FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT   SG+ + D +TG  
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600

Query: 632 STPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKY 691
           S+  D+G+GHV P  A++PGLVYD+T  DY+NFLC  NYT + I ++ RR   CD  ++ 
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660

Query: 692 S-VNDLNYPSFAVVFEGALSGQGGGSTVVKH-TRTLTNVG-SPGTYKVSISSETKLVKIS 751
             V +LNYPSF+VVF+     Q G S +  H  RT+TNVG S   Y++ I    +   ++
Sbjct: 661 GHVGNLNYPSFSVVFQ-----QYGESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVT 720

Query: 752 VEPESLSFTKANEKKSYTVTFTTASGSAAPPSTE-GFGRIEWSDGKHVVGSPI 789
           VEPE LSF +  +K S+ V   T     +P +T    G I WSDGK  V SP+
Sbjct: 721 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of MS011477 vs. ExPASy TrEMBL
Match: A0A6J1IIG3 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3 SV=1)

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 693/763 (90.83%), Postives = 734/763 (96.20%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYG--RLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 91
           M KP+ IF++FCFF G    + AAA+KKTY+VH+AKY+MP  F+HHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVRIFLLFCFFSGPFMAAAAAAQKKTYVVHMAKYQMPLTFEHHLHWYDASLKSVSDT 60

Query: 92  AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADL 151
           A+MIYAY  VVHGFSTRLT EEA+RLE++P +LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAKRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 152 YPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGA 211
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 212 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAA 271
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 272 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAME 331
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 332 KGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 391
           KGILISCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGKP
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKP 360

Query: 392 LQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 451
           L  TLLPFIYA NASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LSGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 452 GMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 511
           GMVLANTA NGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 512 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSM 571
           PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDERRVDFNIISGTSM 540

Query: 572 SCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGH 631
           SCPHVSGLAAL+KGAH DWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 632 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSF 691
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 692 AVVFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 751
           AVVF+G L G   GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 752 KKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           KKSYTVTFTTA+GS+ PPS EGFGRIEW+DGKHVVGSPIAFSW
Sbjct: 721 KKSYTVTFTTATGSSVPPSAEGFGRIEWTDGKHVVGSPIAFSW 763

BLAST of MS011477 vs. ExPASy TrEMBL
Match: A0A6J1GEZ1 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE=3 SV=1)

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 691/763 (90.56%), Postives = 732/763 (95.94%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMA--AAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 91
           M KP+WIF++FCFF G    A  AA+KKTY+VH+AKY+MP  F+HHLHWYD+SLKSVSDT
Sbjct: 1   MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 92  AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADL 151
           A+MIYAY  VVHGFSTRLT EEA+RLE++P +LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 152 YPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGA 211
           YPES S SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNF+A+NCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 212 RYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAA 271
           R+FSKGYEATLGPIDESKESRSPRDDDGHGTHT+TTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 272 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAME 331
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 332 KGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKP 391
           KGILISCSAGNAGPSPYSLSNT+PWITTVGAGTLDRDFPAYVS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 392 LQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 451
           L  TLLPFIYA NASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 452 GMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 511
           GMVLANTA NGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 512 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSM 571
           PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 572 SCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGH 631
           SCPHVSGLAAL+KGAH DWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 632 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSF 691
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARRD+TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 692 AVVFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 751
           AVVF+G L G   GS+VVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 752 KKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           KKSYTVTFTT +GS+APPS EGFGRIEW+DGKHVVGSPIAFSW
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSW 763

BLAST of MS011477 vs. ExPASy TrEMBL
Match: A0A5D3CR08 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004280 PE=3 SV=1)

HSP 1 Score: 1380.5 bits (3572), Expect = 0.0e+00
Identity = 681/761 (89.49%), Postives = 726/761 (95.40%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAE 91
           MA P+W F++ C F         +KKTYIVH+AKY+MP++F+HHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 92  MIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLYP 151
           MIYAYNNVVHGFSTRLT EEA+RLE++P ILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 152 ESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARY 211
           ESNS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 212 FSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARA 271
           FSKGYEATLGPIDESKESRSPRDDDGHGTHT++TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 272 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG 331
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGG+SDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 332 ILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQ 391
           IL+SCSAGNAGPSP+SLSNT+PWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK L 
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 392 ATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 451
            TLLPFIYA NASN+ NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 452 VLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 511
           VLANTA NGEELVADSHLLPATAVGQKSGDIIRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 512 VAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSC 571
           VAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 572 PHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVD 631
           PHVSGLAAL+KGAHPDWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 632 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFAV 691
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCD+ KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 692 VFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEKK 751
           VF+G L G G GS+VVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 752 SYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           SYTVTF T++GSAAP S EGFGRIEWSDGKHVVGSPIAFSW
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSW 760

BLAST of MS011477 vs. ExPASy TrEMBL
Match: A0A1S3B0Y2 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=1)

HSP 1 Score: 1380.5 bits (3572), Expect = 0.0e+00
Identity = 681/761 (89.49%), Postives = 726/761 (95.40%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAE 91
           MA P+W F++ C F         +KKTYIVH+AKY+MP++F+HHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 92  MIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLYP 151
           MIYAYNNVVHGFSTRLT EEA+RLE++P ILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 152 ESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARY 211
           ESNS SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKLIGAR+
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 212 FSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARA 271
           FSKGYEATLGPIDESKESRSPRDDDGHGTHT++TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 272 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG 331
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGG+SDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 332 ILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQ 391
           IL+SCSAGNAGPSP+SLSNT+PWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK L 
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 392 ATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 451
            TLLPFIYA NASN+ NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 452 VLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 511
           VLANTA NGEELVADSHLLPATAVGQKSGDIIRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 512 VAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSC 571
           VAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 572 PHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVD 631
           PHVSGLAAL+KGAHPDWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 632 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFAV 691
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCD+ KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 692 VFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEKK 751
           VF+G L G G GS+VVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFT ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 752 SYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           SYTVTF T++GSAAP S EGFGRIEWSDGKHVVGSPIAFSW
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSW 760

BLAST of MS011477 vs. ExPASy TrEMBL
Match: A0A0A0KIG4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1)

HSP 1 Score: 1376.3 bits (3561), Expect = 0.0e+00
Identity = 678/761 (89.09%), Postives = 727/761 (95.53%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAE 91
           MA P+W+F++ CFF    SMA  +KKTYIVH+AKY+MP++F+HHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWMFLLLCFF-SVPSMAVGDKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 92  MIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLYP 151
           MIYAYNNVVHGFSTRLT EEA+RLE++P ILAVVPEM YELHTTR+P+FLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMIYELHTTRSPEFLGLDKNANLYP 120

Query: 152 ESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARY 211
           ESNS SEVIIGVLDTG+ PESKSFDDTGLGPVPSSWKGECESGTNF+A+NCNRKL+GAR+
Sbjct: 121 ESNSVSEVIIGVLDTGISPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLVGARF 180

Query: 212 FSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARA 271
           FSKGYEATLGPIDESKESRSPRDDDGHGTHT++TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 272 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG 331
           RVAAYKVCWAGGCFSSDI+AAI+KAV+DNVNVLSMSLGGG+SDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAIDKAVDDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 332 ILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQ 391
           IL+SCSAGNAGPSP+SLSNT+PWITTVGAGTLDRDFPAYVSLGD KNFSGVSLYRGK L 
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 392 ATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 451
            TLLPFIYA NASN+ NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 452 VLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 511
           VLANTA NGEELVADSHLLPATAVGQKSGD IRKYL+S+P+PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDTIRKYLVSDPSPTVTILFEGTKLGIEPSPV 480

Query: 512 VAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSC 571
           VAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 572 PHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVD 631
           PHVSGLAAL+KGAHPDWSPAAIRSALMTTAYTAYK+GQK+QD+ATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 632 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFAV 691
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR+DFTCD+ KKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 692 VFEGALSGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEKK 751
           VFEG L G G GS+VVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESLSFT AN+KK
Sbjct: 661 VFEGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKK 720

Query: 752 SYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           SYTVTFTT + SAAP S E FGRIEWSDGKHVVGSPIAFSW
Sbjct: 721 SYTVTFTTTTSSAAPTSAEAFGRIEWSDGKHVVGSPIAFSW 760

BLAST of MS011477 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 1112.8 bits (2877), Expect = 0.0e+00
Identity = 543/755 (71.92%), Postives = 637/755 (84.37%), Query Frame = 0

Query: 39  FVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAEMIYAYNN 98
           F++ C  +  +S +++++ TYIVH+AK +MP +FD H +WYDSSL+S+SD+AE++Y Y N
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYEN 71

Query: 99  VVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKN-ADLYPESNSAS 158
            +HGFSTRLT EEA  L ++P +++V+PE RYELHTTRTP FLGLD++ ADL+PE+ S S
Sbjct: 72  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 131

Query: 159 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARYFSKGYE 218
           +V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNFTA+ CNRKLIGAR+F++GYE
Sbjct: 132 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 191

Query: 219 ATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARARVAAYK 278
           +T+GPIDESKESRSPRDDDGHGTHTS+TAAGSVVE ASL GYASGTARGMA RARVA YK
Sbjct: 192 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 251

Query: 279 VCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGILISCS 338
           VCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+GIL+SCS
Sbjct: 252 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 311

Query: 339 AGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQATLLPF 398
           AGNAGPS  SLSN APWITTVGAGTLDRDFPA   LG+GKNF+GVSL++G+ L   LLPF
Sbjct: 312 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 371

Query: 399 IYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTA 458
           IYAGNASNA+NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM+LANTA
Sbjct: 372 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 431

Query: 459 TNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSS 518
            NGEELVAD+HLLPAT VG+K+GDIIR Y+ ++PNPT +I   GT +G++PSPVVAAFSS
Sbjct: 432 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSS 491

Query: 519 RGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSCPHVSGL 578
           RGPNSITP +LKPD+IAPGVNILA W+ A GP+GLA D RRV+FNIISGTSMSCPHVSGL
Sbjct: 492 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 551

Query: 579 AALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVDPVSALN 638
           AAL+K  HP+WSPAAIRSALMTTAY  YK G+ L D+ATGKPSTPFDHGAGHV P +A N
Sbjct: 552 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATN 611

Query: 639 PGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYSVNDLNYPSFAVVFEGAL 698
           PGL+YDLT +DYL FLCALNYTS QI S++RR++TCD  K YSV DLNYPSFAV  +G  
Sbjct: 612 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV- 671

Query: 699 SGQGGGSTVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEKKSYTVTF 758
                     K+TRT+T+VG  GTY V ++SET  VKISVEP  L+F +ANEKKSYTVTF
Sbjct: 672 -------GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF 731

Query: 759 TTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           T    S+ P  +  FG IEWSDGKHVVGSP+A SW
Sbjct: 732 TV--DSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MS011477 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 815.1 bits (2104), Expect = 5.0e-236
Identity = 422/759 (55.60%), Postives = 545/759 (71.81%), Query Frame = 0

Query: 38  IFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDTAEMIYAYN 97
           I +I  F +  L +    KKTYI+ V     P++F  H  WY S L S S    ++Y Y 
Sbjct: 11  ITIITTFLF--LLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYT 70

Query: 98  NVVHGFSTRLTPEEA-RRLESRPEILAVVPEMRYELHTTRTPQFLGLDKNADLYPESNSA 157
              HGFS  L   EA   L S   IL +  +  Y LHTTRTP+FLGL+    ++   +S+
Sbjct: 71  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSS 130

Query: 158 SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIGARYFSKGY 217
           + VIIGVLDTGVWPES+SFDDT +  +PS WKGECESG++F +  CN+KLIGAR FSKG+
Sbjct: 131 NGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGF 190

Query: 218 E-ATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMAARARVAA 277
           + A+ G     +ES SPRD DGHGTHTSTTAAGS V NAS  GYA+GTARGMA RARVA 
Sbjct: 191 QMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVAT 250

Query: 278 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGILIS 337
           YKVCW+ GCF SDILAA+++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +S
Sbjct: 251 YKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVS 310

Query: 338 CSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGKPLQATLL 397
           CSAGN+GP+  S++N APW+ TVGAGTLDRDFPA+ +LG+GK  +GVSLY G  +    L
Sbjct: 311 CSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPL 370

Query: 398 PFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLAN 457
             +Y  N  N+S+ NLC+ G+L    V GKIV CDRGVN RV+KGAVV+ AGG+GM++AN
Sbjct: 371 ELVY--NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMAN 430

Query: 458 TATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAF 517
           TA +GEELVADSHLLPA AVG+K+GD++R+Y+ S+  PT  ++F+GT L ++PSPVVAAF
Sbjct: 431 TAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAF 490

Query: 518 SSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDFNIISGTSMSCPHVS 577
           SSRGPN++TP++LKPD+I PGVNILAGWS A+GP+GL  D RR  FNI+SGTSMSCPH+S
Sbjct: 491 SSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHIS 550

Query: 578 GLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPSTPFDHGAGHVDPVSA 637
           GLA L+K AHP+WSP+AI+SALMTTAY    +   L D A    S P+ HG+GHVDP  A
Sbjct: 551 GLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKA 610

Query: 638 LNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKYS-VNDLNYPSFAVVFE 697
           L+PGLVYD++ ++Y+ FLC+L+YT   I ++ +R  + +  KK+S    LNYPSF+V+F 
Sbjct: 611 LSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRP-SVNCSKKFSDPGQLNYPSFSVLF- 670

Query: 698 GALSGQGGGSTVVKHTRTLTNVGSPGT-YKVSISSETKLVKISVEPESLSFTKANEKKSY 757
                  GG  VV++TR +TNVG+  + YKV+++     V ISV+P  LSF    EKK Y
Sbjct: 671 -------GGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRY 730

Query: 758 TVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           TVTF +  G +     E FG I WS+ +H V SP+AFSW
Sbjct: 731 TVTFVSKKGVSMTNKAE-FGSITWSNPQHEVRSPVAFSW 751

BLAST of MS011477 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 781.9 bits (2018), Expect = 4.7e-226
Identity = 399/780 (51.15%), Postives = 529/780 (67.82%), Query Frame = 0

Query: 32  MAKPLWIFVI---FCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVS- 91
           + KP    ++     F     +   + KKTY++H+ K  MP  + +HL WY S + SV+ 
Sbjct: 7   LQKPFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQ 66

Query: 92  --------DTAEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQ 151
                   +   ++Y Y    HG + +LT EEA RLE    ++AV+PE RYELHTTR+P 
Sbjct: 67  HKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPT 126

Query: 152 FLGLDKNAD--LYPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNF 211
           FLGL++     ++ E  +  +V++GVLDTG+WPES+SF+DTG+ PVP++W+G CE+G  F
Sbjct: 127 FLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRF 186

Query: 212 TAANCNRKLIGARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLF 271
              NCNRK++GAR F +GYEA  G IDE  E +SPRD DGHGTHT+ T AGS V+ A+LF
Sbjct: 187 LKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLF 246

Query: 272 GYASGTARGMAARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYK 331
           G+A GTARGMA +ARVAAYKVCW GGCFSSDIL+A+++AV D V VLS+SLGGG+S Y +
Sbjct: 247 GFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSR 306

Query: 332 DSVATGAFAAMEKGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGK 391
           DS++   F AME G+ +SCSAGN GP P SL+N +PWITTVGA T+DRDFPA V +G  +
Sbjct: 307 DSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMR 366

Query: 392 NFSGVSLYRGKPL--QATLLPFIYAG-NASNASNGNLCMTGTLIPEKVAGKIVFCDRGVN 451
            F GVSLY+G+ +  +    P +Y G NAS+    + C+ G L    VAGKIV CDRGV 
Sbjct: 367 TFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVT 426

Query: 452 PRVQKGAVVKAAGGIGMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPT 511
           PRVQKG VVK AGGIGMVL NTATNGEELVADSH+LPA AVG+K G +I++Y ++    T
Sbjct: 427 PRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKAT 486

Query: 512 VTILFEGTKLGIEPSPVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAI 571
            ++   GT++GI+PSPVVAAFSSRGPN ++ ++LKPD++APGVNILA W+  + PS L+ 
Sbjct: 487 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 546

Query: 572 DDRRVDFNIISGTSMSCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDL 631
           D RRV FNI+SGTSMSCPHVSG+AAL+K  HPDWSPAAI+SALMTTAY      + L D 
Sbjct: 547 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 606

Query: 632 ATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARR-DFTC 691
           +   PS+P+DHGAGH+DP+ A +PGLVYD+   +Y  FLC  + + SQ+    +  + TC
Sbjct: 607 SGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC 666

Query: 692 DAGKKYSVNDLNYPSFAVVFEGALSGQGGGSTVVKHTRTLTNVGSP-GTYKVSISSETKL 751
                 +  +LNYP+ + +F      +      +   RT+TNVG    +YKVS+ S  K 
Sbjct: 667 KHTLAKNPGNLNYPAISALFP-----ENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKG 726

Query: 752 VKISVEPESLSFTKANEKKSYTVTFTTASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
             ++V+P++L+FT  ++K SYTVTF T      P     FG + W    H V SP+  +W
Sbjct: 727 ASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE----FGGLVWKSTTHKVRSPVIITW 776

BLAST of MS011477 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 781.2 bits (2016), Expect = 8.0e-226
Identity = 411/779 (52.76%), Postives = 534/779 (68.55%), Query Frame = 0

Query: 30  IAMAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSVSDT 89
           ++++   ++F +   F+   S ++   ++YIVHV +   P  F  H +W+ S L+S+  +
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSS 63

Query: 90  ---AEMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGLDKN 149
              A ++Y+Y+  VHGFS RL+P +   L   P +++V+P+   E+HTT TP FLG  +N
Sbjct: 64  PQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN 123

Query: 150 ADLYPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKL 209
           + L+  SN   +VI+GVLDTG+WPE  SF D+GLGP+PS+WKGECE G +F A++CNRKL
Sbjct: 124 SGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKL 183

Query: 210 IGARYFSKGYEATLGPIDE--SKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTA 269
           IGAR F +GY        +  +KESRSPRD +GHGTHT++TAAGSVV NASL+ YA GTA
Sbjct: 184 IGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTA 243

Query: 270 RGMAARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGISDYYKDSVAT 329
            GMA++AR+AAYK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A 
Sbjct: 244 TGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAI 303

Query: 330 GAFAAMEKGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGV 389
           GAF A   GI++SCSAGN+GP+P + +N APWI TVGA T+DR+F A    GDGK F+G 
Sbjct: 304 GAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGT 363

Query: 390 SLYRGKPLQATLLPFIYAGNASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAV 449
           SLY G+ L  + L  +Y+G+  +     LC  G L    V GKIV CDRG N RV+KG+ 
Sbjct: 364 SLYAGESLPDSQLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 423

Query: 450 VKAAGGIGMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGT 509
           VK AGG GM+LANTA +GEEL ADSHL+PAT VG K+GD IR Y+ +  +PT  I F GT
Sbjct: 424 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 483

Query: 510 KLG-IEPSPVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRVDF 569
            +G   PSP VAAFSSRGPN +TP +LKPD+IAPGVNILAGW+  VGP+ L ID RRV F
Sbjct: 484 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 543

Query: 570 NIISGTSMSCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKPST 629
           NIISGTSMSCPHVSGLAAL++ AHPDWSPAAI+SAL+TTAY    SG+ ++DLATGK S 
Sbjct: 544 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 603

Query: 630 PFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDF---TCDAGKK 689
            F HGAGHVDP  ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C+  K 
Sbjct: 604 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 663

Query: 690 YSVNDLNYPSFAVVFEGALSGQGGGSTVVKHTRTLTNVGS--PGTYKVSISSETKLVKIS 749
            +  DLNYPSF+VVF  A +G+     VVK+ R + NVGS     Y+V + S    V+I 
Sbjct: 664 RTAGDLNYPSFSVVF--ASTGE-----VVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEID 723

Query: 750 VEPESLSFTKANEKKSYTVTFTT---ASGSAAPPSTEGFGRIEWSDGKHVVGSPIAFSW 793
           V P  L+F+K      Y VTF +     G  + P  E FG IEW+DG+HVV SP+A  W
Sbjct: 724 VSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHE-FGSIEWTDGEHVVKSPVAVQW 769

BLAST of MS011477 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 766.5 bits (1978), Expect = 2.0e-221
Identity = 399/773 (51.62%), Postives = 525/773 (67.92%), Query Frame = 0

Query: 32  MAKPLWIFVIFCFFYGRLSMAAAEKKTYIVHVAKYRMPDAFDHHLHWYDSSLKSV-SDTA 91
           MA   + F +        S +++   TYIVHV     P  F  H HWY SSL S+ S   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60

Query: 92  EMIYAYNNVVHGFSTRLTPEEARRLESRPEILAVVPEMRYELHTTRTPQFLGL--DKNAD 151
            +I+ Y+ V HGFS RLT ++A +L   P +++V+PE    LHTTR+P+FLGL     A 
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120

Query: 152 LYPESNSASEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFTAANCNRKLIG 211
           L  ES+  S+++IGV+DTGVWPE  SFDD GLGPVP  WKG+C +  +F  + CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180

Query: 212 ARYFSKGYEATLGPIDESKESRSPRDDDGHGTHTSTTAAGSVVENASLFGYASGTARGMA 271
           AR+F  GYEAT G ++E+ E RSPRD DGHGTHT++ +AG  V  AS  GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240

Query: 272 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAM 331
            +AR+AAYKVCW  GC+ SDILAA + AV D V+V+S+S+GG +  YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300

Query: 332 EKGILISCSAGNAGPSPYSLSNTAPWITTVGAGTLDRDFPAYVSLGDGKNFSGVSLYRGK 391
           ++GI +S SAGN GP   +++N APW+TTVGAGT+DRDFPA V LG+GK  SGVS+Y G 
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360

Query: 392 PLQ-ATLLPFIYAGN--ASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 451
            L    + P +Y G+    +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+ 
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420

Query: 452 AGGIGMVLANTATNGEELVADSHLLPATAVGQKSGDIIRKYLI------SEPNPTVTILF 511
            GG+GM++AN   +GE LVAD H+LPAT+VG   GD IR+Y+       S  +PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480

Query: 512 EGTKLGIEPSPVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDDRRV 571
           +GT+LGI P+PVVA+FS+RGPN  TP++LKPD+IAPG+NILA W   +GPSG+  D+RR 
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540

Query: 572 DFNIISGTSMSCPHVSGLAALVKGAHPDWSPAAIRSALMTTAYTAYKSGQKLQDLATGKP 631
           +FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT   SG+ + D +TG  
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600

Query: 632 STPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARRDFTCDAGKKY 691
           S+  D+G+GHV P  A++PGLVYD+T  DY+NFLC  NYT + I ++ RR   CD  ++ 
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660

Query: 692 S-VNDLNYPSFAVVFEGALSGQGGGSTVVKH-TRTLTNVG-SPGTYKVSISSETKLVKIS 751
             V +LNYPSF+VVF+     Q G S +  H  RT+TNVG S   Y++ I    +   ++
Sbjct: 661 GHVGNLNYPSFSVVFQ-----QYGESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVT 720

Query: 752 VEPESLSFTKANEKKSYTVTFTTASGSAAPPSTE-GFGRIEWSDGKHVVGSPI 789
           VEPE LSF +  +K S+ V   T     +P +T    G I WSDGK  V SP+
Sbjct: 721 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767