MS011450 (gene) Bitter gourd (TR) v1

BLAST of MS011450 vs. NCBI nr
Match: XP_022132992.1 (small RNA degrading nuclease 1 [Momordica charantia])

HSP 1 Score: 1003.0 bits (2592), Expect = 9.0e-289
Identity = 510/511 (99.80%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK
Sbjct: 72  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE
Sbjct: 132 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK
Sbjct: 192 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEEA 360
           EDTPQIGMEKLLLHR+PIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEEA
Sbjct: 312 EDTPQIGMEKLLLHRVPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEEA 371

Query: 361 QQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVIE 420
           QQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVIE
Sbjct: 372 QQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVIE 431

Query: 421 NLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLKE 480
           NLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLKE
Sbjct: 432 NLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLKE 491

Query: 481 ELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           ELRRKDYELSILHKAITIVKKDNKKRKGKKK
Sbjct: 492 ELRRKDYELSILHKAITIVKKDNKKRKGKKK 522

BLAST of MS011450 vs. NCBI nr
Match: XP_038881525.1 (small RNA degrading nuclease 3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 873.2 bits (2255), Expect = 1.1e-249
Identity = 439/513 (85.58%), Postives = 480/513 (93.57%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGM+G EGDWKEFLNSHDPKFGA+MSDPSKR ND+LIAFLKTF+KEDD+
Sbjct: 12  VLVEMVRLTQKRGMKGSEGDWKEFLNSHDPKFGASMSDPSKRPNDLLIAFLKTFNKEDDM 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDK++QCNEKRNFVKQVSQ T + ESPEQ+LVRLTLEHPQYPLEYSFPSYDK WIVTK
Sbjct: 72  MFLDKIIQCNEKRNFVKQVSQKTPENESPEQKLVRLTLEHPQYPLEYSFPSYDKEWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKA KSNS+YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRK+I DYRT+
Sbjct: 132 FSQKSKAPKSNSIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKAITDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPG-TILVGHSLNNDLHALKLDHARVIDTSFIF 240
           ITGISPGDLDGV+CSL D+QKS+ KLLS G TILVGHSLNNDL ALKLDH+RVIDTSFIF
Sbjct: 192 ITGISPGDLDGVSCSLADVQKSITKLLSHGRTILVGHSLNNDLRALKLDHSRVIDTSFIF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVA 300
           KYSNGSIYRRPSL+KLCKS+LGY+LRKEGAPHNCL DA+AAMKLVL+KLESK DD I +A
Sbjct: 252 KYSNGSIYRRPSLSKLCKSILGYDLRKEGAPHNCLDDARAAMKLVLAKLESKADDAIAIA 311

Query: 301 DEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPE 360
           DEDTPQ GMEKLLLHRIPIHVPSE LAGVIRGDFTIELKP KKGQ GDIYSAFA+FKNP+
Sbjct: 312 DEDTPQTGMEKLLLHRIPIHVPSETLAGVIRGDFTIELKPPKKGQGGDIYSAFAVFKNPK 371

Query: 361 EAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEV 420
           EAQQAFE++EGS D+DSSGRPQKLI+FQ  SGS VSIYVRKMGQ DFA+KFSS+KRDLEV
Sbjct: 372 EAQQAFEEIEGSADEDSSGRPQKLIRFQ-HSGSIVSIYVRKMGQKDFADKFSSRKRDLEV 431

Query: 421 IENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESL 480
           IENLVM+KKQKTD  + +ETIANSSRCCDHVEE+E+LKQELKQKEDS+HCCDHLKEVESL
Sbjct: 432 IENLVMAKKQKTDHKMEMETIANSSRCCDHVEEVEKLKQELKQKEDSNHCCDHLKEVESL 491

Query: 481 KEELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           KEELRRKDYEL IL KAI IVKKD++++KGKK+
Sbjct: 492 KEELRRKDYELGILRKAIAIVKKDSRRKKGKKR 523

BLAST of MS011450 vs. NCBI nr
Match: KAG7034323.1 (Small RNA degrading nuclease 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 866.7 bits (2238), Expect = 1.0e-247
Identity = 439/511 (85.91%), Postives = 475/511 (92.95%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFL S DPKFGA+M DPSKR ND+LIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLISRDPKFGASMCDPSKRPNDLLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDK+LQCNEKRN VKQVSQ TSD ESPEQRLVRLTLEHPQYPLEYSFPSY+KGWIVTK
Sbjct: 72  MFLDKILQCNEKRNVVKQVSQKTSDNESPEQRLVRLTLEHPQYPLEYSFPSYEKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSN++YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELV PRK+IKDYRT+
Sbjct: 132 FSQKSKALKSNAIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVNPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDGVTCSL D+Q+S+ KLLS GTIL GHSLNNDL ALKLDH RVIDTSFIFK
Sbjct: 192 ITGISPGDLDGVTCSLVDVQRSITKLLSHGTILAGHSLNNDLQALKLDHTRVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGY+LR+EGA HNCL DA+AAMKLVL+KLESK DDVI + D
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYDLREEGASHNCLDDARAAMKLVLAKLESKADDVIAIVD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPEE 360
           EDTPQIGMEKLLLHRIPI+VPSE LAGVI GDFTIELKP KKGQ GDIYSAFAIFKNP+E
Sbjct: 312 EDTPQIGMEKLLLHRIPINVPSEALAGVIPGDFTIELKPPKKGQGGDIYSAFAIFKNPKE 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVI 420
           A+QAFEQ+EG+P++DSSGRPQKL++FQ  SGS VSI+VR MG NDFAEK SS+KRDLEVI
Sbjct: 372 ARQAFEQIEGNPNEDSSGRPQKLVRFQ-RSGSIVSIFVRTMGANDFAEKVSSRKRDLEVI 431

Query: 421 ENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLK 480
           ENLVMSKKQKTDQ I +ETI+ SSRCC+HVEE+ERLKQELKQKEDSSHCCDHLKEVESLK
Sbjct: 432 ENLVMSKKQKTDQKIEMETISKSSRCCNHVEEVERLKQELKQKEDSSHCCDHLKEVESLK 491

Query: 481 EELRRKDYELSILHKAITIVKKDNKKRKGKK 511
           E+LRRKDYEL+IL KAIT+ KK+N+KRKGKK
Sbjct: 492 EDLRRKDYELNILRKAITVGKKENRKRKGKK 521

BLAST of MS011450 vs. NCBI nr
Match: KAG6604160.1 (Small RNA degrading nuclease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 865.1 bits (2234), Expect = 2.9e-247
Identity = 438/511 (85.71%), Postives = 474/511 (92.76%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFL S DPKFGA+M DPSKR ND+LIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLISRDPKFGASMCDPSKRPNDLLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDK+LQCNEKRN VKQVSQ TSD ESPEQRLVRLTLEHPQYPLEYSFPSY+KGWIVTK
Sbjct: 72  MFLDKILQCNEKRNVVKQVSQKTSDNESPEQRLVRLTLEHPQYPLEYSFPSYEKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSN++YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELV PRK+IKDYRT+
Sbjct: 132 FSQKSKALKSNAIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVNPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDGVTCSL D+Q+S+ KLLS GTIL GHSLNNDL ALKLDH RVIDTSFIFK
Sbjct: 192 ITGISPGDLDGVTCSLVDVQRSITKLLSHGTILAGHSLNNDLQALKLDHTRVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGY+LR+EGA HNCL DA+AAMKLVL+KLESK DDVI + D
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYDLREEGASHNCLDDARAAMKLVLAKLESKADDVIAIVD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPEE 360
           EDTPQIGMEKL LHRIPI+VPSE LAGVI GDFTIELKP KKGQ GDIYSAFAIFKNP+E
Sbjct: 312 EDTPQIGMEKLFLHRIPINVPSEALAGVIPGDFTIELKPPKKGQGGDIYSAFAIFKNPKE 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVI 420
           A+QAFEQ+EG+P++DSSGRPQKL++FQ  SGS VSI+VR MG NDFAEK SS+KRDLEVI
Sbjct: 372 ARQAFEQIEGNPNEDSSGRPQKLVRFQ-RSGSIVSIFVRTMGANDFAEKVSSRKRDLEVI 431

Query: 421 ENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLK 480
           ENLVMSKKQKTDQ I +ETI+ SSRCC+HVEE+ERLKQELKQKEDSSHCCDHLKEVESLK
Sbjct: 432 ENLVMSKKQKTDQKIEMETISKSSRCCNHVEEVERLKQELKQKEDSSHCCDHLKEVESLK 491

Query: 481 EELRRKDYELSILHKAITIVKKDNKKRKGKK 511
           E+LRRKDYEL+IL KAIT+ KK+N+KRKGKK
Sbjct: 492 EDLRRKDYELNILRKAITVGKKENRKRKGKK 521

BLAST of MS011450 vs. NCBI nr
Match: XP_022977561.1 (small RNA degrading nuclease 3-like [Cucurbita maxima])

HSP 1 Score: 863.6 bits (2230), Expect = 8.5e-247
Identity = 437/511 (85.52%), Postives = 475/511 (92.95%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFL S DPKFGA+M DPSKR ND+LIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLISRDPKFGASMCDPSKRPNDLLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDK+LQCNEKRN VKQVSQ TSD ESPEQRLVRLTLEHPQYPLEYSFPSY+KGWIVTK
Sbjct: 72  MFLDKILQCNEKRNVVKQVSQKTSDNESPEQRLVRLTLEHPQYPLEYSFPSYEKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSN++YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELV PRK+IKDYRT+
Sbjct: 132 FSQKSKALKSNAIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVNPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDGVTCSL D+Q+S+ KLLS GTIL GHSLNNDL ALKLDH RVIDTSFIFK
Sbjct: 192 ITGISPGDLDGVTCSLVDVQRSITKLLSHGTILAGHSLNNDLQALKLDHTRVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGY+LR+EGA HNCL DA+AAMKLVL+KLESK DDVI + D
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYDLREEGASHNCLDDARAAMKLVLAKLESKADDVIAIVD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPEE 360
           EDTPQIGMEKLLLHRIPI+VPSE LAGVI GDFTIELKP K+GQ GDIYSAFA+FKN +E
Sbjct: 312 EDTPQIGMEKLLLHRIPINVPSEALAGVIPGDFTIELKPPKRGQGGDIYSAFAVFKNSKE 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVI 420
           A+QAFEQ+EG+P++DSSGRPQKL++FQ  SGS VSI+VR MG NDFAEK SS+KRDLEVI
Sbjct: 372 ARQAFEQIEGNPNEDSSGRPQKLVRFQ-RSGSIVSIFVRTMGANDFAEKVSSRKRDLEVI 431

Query: 421 ENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLK 480
           E+LVMSKKQKTDQ I +ETI+NSSRCC+HVEE+ERLKQELKQKEDSSHCCDHLKEVESLK
Sbjct: 432 ESLVMSKKQKTDQKIEMETISNSSRCCNHVEEVERLKQELKQKEDSSHCCDHLKEVESLK 491

Query: 481 EELRRKDYELSILHKAITIVKKDNKKRKGKK 511
           E+LRRKDYELSIL KAIT+ KK+N+KRKGKK
Sbjct: 492 EDLRRKDYELSILRKAITVGKKENRKRKGKK 521

BLAST of MS011450 vs. ExPASy Swiss-Prot
Match: Q8RXK2 (Small RNA degrading nuclease 3 OS=Arabidopsis thaliana OX=3702 GN=SDN3 PE=1 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.3e-120
Identity = 228/442 (51.58%), Postives = 309/442 (69.91%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           +LV++V+L QKRG+ G  G WKEFLN +D K G+++SDP++RSND+L+AFL TF K++DL
Sbjct: 12  LLVDLVKLVQKRGLEGENGGWKEFLNVYDKKLGSSLSDPARRSNDVLVAFLLTFKKKEDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
             + +V+QC   R  +++  Q T DKE+PEQRLVRLT+ H  YP  Y+FPSY + W VT+
Sbjct: 72  QLIARVMQCGANRELIEKFKQETPDKETPEQRLVRLTITHDDYPGNYTFPSYAEDWYVTE 131

Query: 121 F-SQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              +KSK +KS  M +IDCEMV CEDG++ LVRV  VDRDL+V +D+ VKP K + DY+T
Sbjct: 132 LGKKKSKVIKSTRMLSIDCEMVTCEDGSQALVRVGAVDRDLKVVLDKFVKPDKPVIDYKT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
           +ITG++  DL+  T S+ DIQK +++ LS GTILVGH L+NDL  L++DHARVIDTS++F
Sbjct: 192 DITGVTAEDLERATLSVADIQKKLRRFLSVGTILVGHGLHNDLQVLRIDHARVIDTSYVF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVI--- 300
           ++ +    +RPSLN LCKSVLG E+R +GA HNC+HDA AAMKLVL+ +E     +I   
Sbjct: 252 EFVDAPKTQRPSLNNLCKSVLGQEVRMDGAAHNCVHDAAAAMKLVLAAVEKGAATLIQPT 311

Query: 301 ---VVAD----EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYS 360
              +VA+    E   + G  +L LH+IP  VPSE+L GV+ G+FT+ +KP K   G   +
Sbjct: 312 EEMMVAEKRRQEARQEAGKAQLFLHKIPHDVPSEELHGVLSGNFTLVVKPPK--TGGYST 371

Query: 361 AFAIFKNPEEAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKF 420
           A   F +PEEA +AFE VEG   KD SG PQK    +LSSG  VS++VRKM Q+D   + 
Sbjct: 372 AVVDFSSPEEANEAFENVEGDVAKDKSGLPQKKAVLKLSSGLAVSLFVRKMVQDDSPCEI 431

Query: 421 SSKKRDLEVIENLVMSKKQKTD 432
           S+ +R     EN V SK+QKT+
Sbjct: 432 STSER-ARAEENNVSSKRQKTE 450

HSP 2 Score: 44.3 bits (103), Expect = 4.9e-03
Identity = 33/113 (29.20%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 400 MGQNDFAEKFSSKKRDLEVIENLVMSKKQKTDQNIAVETIANSSRC-CDHVEEIERLKQE 459
           + Q   A K SS       +  +V  + ++T +       AN++ C  DH++E+E LK++
Sbjct: 676 LAQKMVAFKLSSGSGACLYVRKMVQDESEETKE-------ANANHCEDDHLKEMEELKEK 735

Query: 460 LKQKEDSSHCCDHLKEVESLKEELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           LK  E +  C  H KE+E LK++L  K++++    K I  +K   +K++ K +
Sbjct: 736 LKAMEFAISCEGHSKEIEELKQKLNAKEHQIQAQDKIIANLKMKLEKKQSKSR 781

BLAST of MS011450 vs. ExPASy Swiss-Prot
Match: A3KPE8 (Small RNA degrading nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=SDN1 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 7.5e-113
Identity = 210/405 (51.85%), Postives = 288/405 (71.11%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VL E+V+L Q R +RG  G+WKEFL+ +D K   + SDPS+RS++ L+ FL TF K++DL
Sbjct: 12  VLDELVKLLQSRDLRGENGNWKEFLHVYD-KNADSPSDPSRRSHEDLVQFLTTFKKKEDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
               ++L+C+     ++ + Q + D+++PEQ LVRLT+EHP Y L+YSF  Y + W V+ 
Sbjct: 72  ----QLLKCHANHLLIENLKQESQDEDTPEQMLVRLTVEHPSYSLDYSFKPYSEDWFVSD 131

Query: 121 FSQK-SKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              K  K ++S +M A+DCEMVLCEDGTE LVRV +VDRDL+V +DE VKP K + DYRT
Sbjct: 132 VGMKMKKVMESTNMVAVDCEMVLCEDGTEGLVRVGVVDRDLKVILDEFVKPNKPVVDYRT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
           +ITGI+  D++  + S+ DIQ++++  LS GTILVGHSLN DL  LK+DH +VIDT+ +F
Sbjct: 192 DITGITAEDIENASLSVVDIQETLQPFLSTGTILVGHSLNRDLEVLKIDHPKVIDTALVF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVA 300
           KY N    RRPSLN LCKS+LGYE+RK G PH+C+HDA AAMKL L+ +E +VD  I  +
Sbjct: 252 KYPNTRKLRRPSLNNLCKSILGYEVRKTGVPHDCVHDASAAMKLALAVVEKRVDTTIKPS 311

Query: 301 DEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEE 360
            E   ++   KL LH+IP +VPSE+L  V+ G FT+++K +K  QG  Y AFA+F + E+
Sbjct: 312 KE-MLEVEKAKLFLHKIPNNVPSEELEQVLSGKFTLDVKQAKT-QGRYYCAFALFHSSED 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQND 405
           A QAFE ++G    DS G PQK++  +LSSGS  SIYVRKM Q++
Sbjct: 372 ADQAFEHIDGIEMTDSLGLPQKVVIIKLSSGSRASIYVRKMVQDE 409

BLAST of MS011450 vs. ExPASy Swiss-Prot
Match: Q9FFG1 (Small RNA degrading nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=SDN2 PE=2 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.6e-102
Identity = 211/440 (47.95%), Postives = 285/440 (64.77%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFG-ANMSDPSKRSNDMLIAFLKTFSKEDD 60
           VLV++V++AQK G++G  G WKEFL+ +D + G +++SDPSKR  D L+AFL T  K++D
Sbjct: 12  VLVKLVKVAQKLGLKGENGTWKEFLDFYDKQLGSSSLSDPSKRRKDDLVAFLTTLKKKED 71

Query: 61  LMFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVT 120
           L  L K L+ +   N V +  +  S  E+ EQRLVR+TL H +YPL+Y FPS  + W+ T
Sbjct: 72  LQLLAKSLKLD---NDVFEKFKKKSLDETAEQRLVRMTLTHDEYPLDYLFPSNAEDWVRT 131

Query: 121 KFSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              +K        M AIDCEMVLCEDG+E +VRV  VDRDL+V +DE VKP + + DYRT
Sbjct: 132 GLGKKKMEPTKIEMIAIDCEMVLCEDGSEAVVRVAAVDRDLKVILDEFVKPNQPVVDYRT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
            ITG++  DL+  T S+ DIQ+ +   +S  TILVG SLN+DL  LK+DHARVIDTS +F
Sbjct: 192 FITGLTAQDLEKATISVVDIQEKLLMFISEDTILVGQSLNHDLKVLKVDHARVIDTSLVF 251

Query: 241 KYSNGSIYR-----RPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDD 300
           KY+     R     RPSLN LCK +LGYE++KEG PHNC+HDA+AAMKLVL+ L++  + 
Sbjct: 252 KYNYDGTRRPLRLKRPSLNYLCKCILGYEVQKEGVPHNCVHDAEAAMKLVLAILDNGAET 311

Query: 301 VIVVADEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIF 360
            + ++ E   +    KL LHRIP +VP E+L GV+  D   E+KPSKK     YSA  +F
Sbjct: 312 SVPLSKE-MLEAEKSKLYLHRIPCNVPYEELNGVVSRDIPHEVKPSKKQDRHYYSAIVVF 371

Query: 361 KNPEEAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGS-TVSIYVRKMGQNDFAEKFSSKK 420
           K+PEEA QAFE + G   KDS G  QK I  + SS    + + VRKM ++D   +  ++ 
Sbjct: 372 KSPEEANQAFENIAGDFGKDSRGLSQKQIFLEPSSSEPRLYVLVRKMVEDDLVGEVIAE- 431

Query: 421 RDLEVIENLVMSKKQKTDQN 434
                 EN   SKK+K + +
Sbjct: 432 ------ENNASSKKRKRENH 440

BLAST of MS011450 vs. ExPASy Swiss-Prot
Match: Q9SN09 (Putative small RNA degrading nuclease 4 OS=Arabidopsis thaliana OX=3702 GN=SDN4 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.1e-71
Identity = 153/323 (47.37%), Postives = 200/323 (61.92%), Query Frame = 0

Query: 73  RNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTKFSQK-SKALKSN 132
           R  + +  + +   +SPEQRLVRLT E+PQY +++ F SY K W V+    K S  +  N
Sbjct: 14  RLMIAKFKKESPANDSPEQRLVRLTNENPQYNVDFLFHSYSKDWFVSDVGMKMSNVMIPN 73

Query: 133 SMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTEITGISPGDLDG 192
            M A+DCEMVLCEDGTE +VRV  VDR+L+V +DE VKP K + DYRT ITG++  D+  
Sbjct: 74  QMLALDCEMVLCEDGTEGVVRVGAVDRNLKVILDEFVKPHKPVVDYRTAITGVTAEDVQK 133

Query: 193 VTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFKYSNGSIYRRPS 252
            T SL DIQ+ ++  LS G IL+             DH  VIDTS +FKY N    RRPS
Sbjct: 134 ATLSLVDIQEKLRPFLSAGAILI-------------DHPIVIDTSLVFKYPNSRKLRRPS 193

Query: 253 LNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVADEDTPQIGMEKL 312
           LN LC SVLGYE++K G  H+C+HDA AAMKL L+ ++ +VD  I +    T +    +L
Sbjct: 194 LNTLCMSVLGYEVQKAGVSHHCVHDAAAAMKLALAVIKKRVDTTITL----TKEAEKSRL 253

Query: 313 LLHRIPIHVPSEDLAGVIR-----GDFTIELKPSKKGQGDIYSAFAIFKNPEEAQQAFEQ 372
            LHRIP H+ SE+L   +       +FTI++KP+K  QG  Y A  IF +  EA QAFE 
Sbjct: 254 FLHRIPHHLSSEELKKDLALKFFPKNFTIDVKPAKT-QGGYYCAVVIFGSSVEANQAFEN 313

Query: 373 VEGSPDKDSSGRPQKLIKFQLSS 390
           V G  + DSSG PQKLI   +S+
Sbjct: 314 VNGYKETDSSGLPQKLISCSIST 318

BLAST of MS011450 vs. ExPASy Swiss-Prot
Match: Q8L7M4 (Small RNA degrading nuclease 5 OS=Arabidopsis thaliana OX=3702 GN=SDN5 PE=2 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 8.2e-27
Identity = 88/254 (34.65%), Postives = 136/254 (53.54%), Query Frame = 0

Query: 58  DDLMFLDKVLQCNEKRNFVKQVSQSTSDK---ESPE------QRLVRLTLEHPQYPLEY- 117
           D++  +D +L C  ++   K V+ S        SPE      +  V LT + P +P+ Y 
Sbjct: 125 DEMRTIDTILTCKGRKK--KTVTSSVEPPPLVSSPEACNLMGKSFVELTKDIP-FPVSYY 184

Query: 118 --SFPSYDKGWIVTKFSQKSKALKSNS------MYAIDCEMVLCEDGTENLVRVCMVDRD 177
             S    ++     +  + +  L + S      + A+DCEM + ++G E L RV +VD  
Sbjct: 185 TLSQKEMEQNGYTFEKLELTPTLPAPSGSCPPEIVALDCEMCITKEGLE-LTRVTLVDIQ 244

Query: 178 LQVKIDELVKPRKSIKDYRTEITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLN 237
            QV +D+LV P   I DY T  +GI+   ++GVT +L DIQ+   KL+   TILVGHSL 
Sbjct: 245 GQVLLDKLVMPTNPITDYNTRYSGITAVMMEGVTTTLKDIQEEFLKLVFKETILVGHSLE 304

Query: 238 NDLHALKLDHARVIDTSFIFKYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQA 294
           NDL +LK+ H  VIDT+ ++K+ +G  Y +  L  L K  L  E+++  + H+   DA+A
Sbjct: 305 NDLLSLKISHNLVIDTAVLYKHPHGRSY-KTKLRILAKKFLAREIQESESGHDSAEDAKA 364

BLAST of MS011450 vs. ExPASy TrEMBL
Match: A0A6J1BVD3 (small RNA degrading nuclease 1 OS=Momordica charantia OX=3673 GN=LOC111005697 PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.3e-289
Identity = 510/511 (99.80%), Postives = 511/511 (100.00%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK
Sbjct: 72  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE
Sbjct: 132 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK
Sbjct: 192 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEEA 360
           EDTPQIGMEKLLLHR+PIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEEA
Sbjct: 312 EDTPQIGMEKLLLHRVPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEEA 371

Query: 361 QQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVIE 420
           QQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVIE
Sbjct: 372 QQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVIE 431

Query: 421 NLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLKE 480
           NLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLKE
Sbjct: 432 NLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLKE 491

Query: 481 ELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           ELRRKDYELSILHKAITIVKKDNKKRKGKKK
Sbjct: 492 ELRRKDYELSILHKAITIVKKDNKKRKGKKK 522

BLAST of MS011450 vs. ExPASy TrEMBL
Match: A0A6J1IKA1 (small RNA degrading nuclease 3-like OS=Cucurbita maxima OX=3661 GN=LOC111477856 PE=4 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.1e-247
Identity = 437/511 (85.52%), Postives = 475/511 (92.95%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFL S DPKFGA+M DPSKR ND+LIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLISRDPKFGASMCDPSKRPNDLLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDK+LQCNEKRN VKQVSQ TSD ESPEQRLVRLTLEHPQYPLEYSFPSY+KGWIVTK
Sbjct: 72  MFLDKILQCNEKRNVVKQVSQKTSDNESPEQRLVRLTLEHPQYPLEYSFPSYEKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSN++YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELV PRK+IKDYRT+
Sbjct: 132 FSQKSKALKSNAIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVNPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDGVTCSL D+Q+S+ KLLS GTIL GHSLNNDL ALKLDH RVIDTSFIFK
Sbjct: 192 ITGISPGDLDGVTCSLVDVQRSITKLLSHGTILAGHSLNNDLQALKLDHTRVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGY+LR+EGA HNCL DA+AAMKLVL+KLESK DDVI + D
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYDLREEGASHNCLDDARAAMKLVLAKLESKADDVIAIVD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPEE 360
           EDTPQIGMEKLLLHRIPI+VPSE LAGVI GDFTIELKP K+GQ GDIYSAFA+FKN +E
Sbjct: 312 EDTPQIGMEKLLLHRIPINVPSEALAGVIPGDFTIELKPPKRGQGGDIYSAFAVFKNSKE 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVI 420
           A+QAFEQ+EG+P++DSSGRPQKL++FQ  SGS VSI+VR MG NDFAEK SS+KRDLEVI
Sbjct: 372 ARQAFEQIEGNPNEDSSGRPQKLVRFQ-RSGSIVSIFVRTMGANDFAEKVSSRKRDLEVI 431

Query: 421 ENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLK 480
           E+LVMSKKQKTDQ I +ETI+NSSRCC+HVEE+ERLKQELKQKEDSSHCCDHLKEVESLK
Sbjct: 432 ESLVMSKKQKTDQKIEMETISNSSRCCNHVEEVERLKQELKQKEDSSHCCDHLKEVESLK 491

Query: 481 EELRRKDYELSILHKAITIVKKDNKKRKGKK 511
           E+LRRKDYELSIL KAIT+ KK+N+KRKGKK
Sbjct: 492 EDLRRKDYELSILRKAITVGKKENRKRKGKK 521

BLAST of MS011450 vs. ExPASy TrEMBL
Match: A0A6J1GE03 (small RNA degrading nuclease 3-like OS=Cucurbita moschata OX=3662 GN=LOC111453298 PE=4 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.4e-247
Identity = 436/511 (85.32%), Postives = 474/511 (92.76%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFL S DPKFGA+M DPSKR ND+LIAFLKTFSKEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLISRDPKFGASMCDPSKRPNDLLIAFLKTFSKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDK+LQCNEKRN VKQVSQ TSD ESPEQRLVRLTLEHPQYPLEYSFPSY+KGWIVTK
Sbjct: 72  MFLDKILQCNEKRNVVKQVSQKTSDNESPEQRLVRLTLEHPQYPLEYSFPSYEKGWIVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSKALKSN++YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELV PRK+IKDYRT+
Sbjct: 132 FSQKSKALKSNAIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVNPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIFK 240
           ITGISPGDLDG+TCSL D+Q+S+ KLLS GTIL GHSLNNDL ALKLDH RVIDTSFIFK
Sbjct: 192 ITGISPGDLDGITCSLVDVQRSITKLLSHGTILAGHSLNNDLQALKLDHTRVIDTSFIFK 251

Query: 241 YSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVAD 300
           YSNGSIYRRPSLNKLCKSVLGY+LR+EGA HNCL DA+AAMKLVL+KLESK DDVI + D
Sbjct: 252 YSNGSIYRRPSLNKLCKSVLGYDLREEGASHNCLDDARAAMKLVLAKLESKADDVIAIVD 311

Query: 301 EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPEE 360
           EDTPQIGMEKL LHRIPI+VPSE LAGVI GDFTIELKP KKGQ GDIYSAFAIFKNP+E
Sbjct: 312 EDTPQIGMEKLFLHRIPINVPSEALAGVIPGDFTIELKPPKKGQGGDIYSAFAIFKNPKE 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEVI 420
           A+QAFEQ+EG+P++DSSGRPQKL++FQ  SGS VSI+VR MG NDFAEK SS+KRDLEVI
Sbjct: 372 ARQAFEQIEGNPNEDSSGRPQKLVRFQ-RSGSIVSIFVRTMGANDFAEKVSSRKRDLEVI 431

Query: 421 ENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESLK 480
           ENLVMSKKQKTDQ I +ETI+ SSRCC+HVEE+ERLKQELKQKEDSSHCCDHLKEVESLK
Sbjct: 432 ENLVMSKKQKTDQKIEMETISKSSRCCNHVEEVERLKQELKQKEDSSHCCDHLKEVESLK 491

Query: 481 EELRRKDYELSILHKAITIVKKDNKKRKGKK 511
           E+LRRKDYEL+IL KAIT+ KK+N+KR+GKK
Sbjct: 492 EDLRRKDYELNILRKAITVGKKENRKREGKK 521

BLAST of MS011450 vs. ExPASy TrEMBL
Match: A0A5D3CPA3 (Small RNA degrading nuclease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004560 PE=4 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 5.0e-245
Identity = 435/513 (84.80%), Postives = 472/513 (92.01%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFLNS DPKFGA+MSDPSKR ND+LIAFLKTF+KEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLNSRDPKFGASMSDPSKRPNDLLIAFLKTFNKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDKVLQCNEKRNFVKQVSQ T   ESPEQ+LVRLTLEHPQYPLEYSFPSYDK W+VTK
Sbjct: 72  MFLDKVLQCNEKRNFVKQVSQITPGNESPEQKLVRLTLEHPQYPLEYSFPSYDKEWVVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSK   SN +YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRK+IKDYRT+
Sbjct: 132 FSQKSKGPNSNLIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPG-TILVGHSLNNDLHALKLDHARVIDTSFIF 240
           ITGISPGDLDGV+CSL D+QKS+ K LS G TILVGHSLNNDL ALKLDH+RVIDTSFIF
Sbjct: 192 ITGISPGDLDGVSCSLADVQKSITKFLSHGRTILVGHSLNNDLQALKLDHSRVIDTSFIF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVA 300
           KYSNGSIYRRPSL+KLCKSVLGY+LRKEGAPHNCL DAQAAMKLVL+KLESK DD I +A
Sbjct: 252 KYSNGSIYRRPSLSKLCKSVLGYDLRKEGAPHNCLDDAQAAMKLVLAKLESKADDTIAIA 311

Query: 301 DEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPE 360
           DEDTPQ+GMEKLLLHRIPI+VPSE LA  I GDFTIELKP KKGQ GDIYSA AIFKNP+
Sbjct: 312 DEDTPQVGMEKLLLHRIPINVPSEALARAIHGDFTIELKPPKKGQGGDIYSALAIFKNPK 371

Query: 361 EAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEV 420
           EAQQAFE++EGS D+DSSGRPQKLI+FQ  SGS VSIYVRKMGQ DFA+KFSSKKRDLEV
Sbjct: 372 EAQQAFEEIEGSADEDSSGRPQKLIRFQ-HSGSIVSIYVRKMGQKDFADKFSSKKRDLEV 431

Query: 421 IENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESL 480
           IEN+VMSKKQKTD  + +ETI+NSS+CC+HVEE+ERLKQELK+KEDS+HCCDH+ EVESL
Sbjct: 432 IENIVMSKKQKTDNKMEIETISNSSKCCNHVEEVERLKQELKRKEDSTHCCDHVNEVESL 491

Query: 481 KEELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           KEELRRKDYELSIL K ITIVKKD+KK+KGKK+
Sbjct: 492 KEELRRKDYELSILRKTITIVKKDSKKKKGKKR 523

BLAST of MS011450 vs. ExPASy TrEMBL
Match: A0A1S3B1K6 (small RNA degrading nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103484801 PE=4 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.4e-242
Identity = 431/512 (84.18%), Postives = 469/512 (91.60%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VLVEMVRL QKRGMRG EGDWKEFLNS DPKFGA+MSDPSKR ND+LIAFLKTF+KEDDL
Sbjct: 12  VLVEMVRLTQKRGMRGSEGDWKEFLNSRDPKFGASMSDPSKRPNDLLIAFLKTFNKEDDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
           MFLDKVLQCNEKRNFVKQVSQ T   ESPEQ+LVRLTLEHPQYPLEYSFPSYDK W+VTK
Sbjct: 72  MFLDKVLQCNEKRNFVKQVSQITPGNESPEQKLVRLTLEHPQYPLEYSFPSYDKEWVVTK 131

Query: 121 FSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRTE 180
           FSQKSK   SN +YAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRK+IKDYRT+
Sbjct: 132 FSQKSKGPNSNLIYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKAIKDYRTD 191

Query: 181 ITGISPGDLDGVTCSLDDIQKSMKKLLSPG-TILVGHSLNNDLHALKLDHARVIDTSFIF 240
           ITGISPGDLDGV+CSL D+QKS+ K LS G TILVGHSLNNDL ALKLDH+RVIDTSFIF
Sbjct: 192 ITGISPGDLDGVSCSLADVQKSITKFLSHGRTILVGHSLNNDLQALKLDHSRVIDTSFIF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVA 300
           KYSNGSIYRRPSL+KLCKSVLGY+LRKEGAPHNCL DAQAAMKLVL+KL SK DD I +A
Sbjct: 252 KYSNGSIYRRPSLSKLCKSVLGYDLRKEGAPHNCLDDAQAAMKLVLAKLGSKADDTIAIA 311

Query: 301 DEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQ-GDIYSAFAIFKNPE 360
           DEDTPQ+GMEKLLLHRIPI+VPSE LA  I GDFTIELKP KKGQ GDIYSA AIFKNP+
Sbjct: 312 DEDTPQVGMEKLLLHRIPINVPSEALARAIHGDFTIELKPPKKGQGGDIYSALAIFKNPK 371

Query: 361 EAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKFSSKKRDLEV 420
           EAQQAFE++EGS D+DSSGRPQKLI+FQ  SGS VSIYVRKMGQ DFA+KFSSKKRDLEV
Sbjct: 372 EAQQAFEEIEGSADEDSSGRPQKLIRFQ-HSGSIVSIYVRKMGQKDFADKFSSKKRDLEV 431

Query: 421 IENLVMSKKQKTDQNIAVETIANSSRCCDHVEEIERLKQELKQKEDSSHCCDHLKEVESL 480
           IEN+VMSKKQKTD  + +ETI+NSS+CC+HVEE+ERLKQELK+KEDS+HCCDH+ EVESL
Sbjct: 432 IENIVMSKKQKTDNKMEIETISNSSKCCNHVEEVERLKQELKRKEDSTHCCDHVNEVESL 491

Query: 481 KEELRRKDYELSILHKAITIVKKDNKKRKGKK 511
           KEELRRKDYELSIL K ITIVKKD+KK+K ++
Sbjct: 492 KEELRRKDYELSILRKTITIVKKDSKKKKERR 522

BLAST of MS011450 vs. TAIR 10
Match: AT5G67240.1 (small RNA degrading nuclease 3 )

HSP 1 Score: 434.9 bits (1117), Expect = 9.1e-122
Identity = 228/442 (51.58%), Postives = 309/442 (69.91%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           +LV++V+L QKRG+ G  G WKEFLN +D K G+++SDP++RSND+L+AFL TF K++DL
Sbjct: 12  LLVDLVKLVQKRGLEGENGGWKEFLNVYDKKLGSSLSDPARRSNDVLVAFLLTFKKKEDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
             + +V+QC   R  +++  Q T DKE+PEQRLVRLT+ H  YP  Y+FPSY + W VT+
Sbjct: 72  QLIARVMQCGANRELIEKFKQETPDKETPEQRLVRLTITHDDYPGNYTFPSYAEDWYVTE 131

Query: 121 F-SQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              +KSK +KS  M +IDCEMV CEDG++ LVRV  VDRDL+V +D+ VKP K + DY+T
Sbjct: 132 LGKKKSKVIKSTRMLSIDCEMVTCEDGSQALVRVGAVDRDLKVVLDKFVKPDKPVIDYKT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
           +ITG++  DL+  T S+ DIQK +++ LS GTILVGH L+NDL  L++DHARVIDTS++F
Sbjct: 192 DITGVTAEDLERATLSVADIQKKLRRFLSVGTILVGHGLHNDLQVLRIDHARVIDTSYVF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVI--- 300
           ++ +    +RPSLN LCKSVLG E+R +GA HNC+HDA AAMKLVL+ +E     +I   
Sbjct: 252 EFVDAPKTQRPSLNNLCKSVLGQEVRMDGAAHNCVHDAAAAMKLVLAAVEKGAATLIQPT 311

Query: 301 ---VVAD----EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYS 360
              +VA+    E   + G  +L LH+IP  VPSE+L GV+ G+FT+ +KP K   G   +
Sbjct: 312 EEMMVAEKRRQEARQEAGKAQLFLHKIPHDVPSEELHGVLSGNFTLVVKPPK--TGGYST 371

Query: 361 AFAIFKNPEEAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKF 420
           A   F +PEEA +AFE VEG   KD SG PQK    +LSSG  VS++VRKM Q+D   + 
Sbjct: 372 AVVDFSSPEEANEAFENVEGDVAKDKSGLPQKKAVLKLSSGLAVSLFVRKMVQDDSPCEI 431

Query: 421 SSKKRDLEVIENLVMSKKQKTD 432
           S+ +R     EN V SK+QKT+
Sbjct: 432 STSER-ARAEENNVSSKRQKTE 450

HSP 2 Score: 44.3 bits (103), Expect = 3.5e-04
Identity = 33/113 (29.20%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 400 MGQNDFAEKFSSKKRDLEVIENLVMSKKQKTDQNIAVETIANSSRC-CDHVEEIERLKQE 459
           + Q   A K SS       +  +V  + ++T +       AN++ C  DH++E+E LK++
Sbjct: 676 LAQKMVAFKLSSGSGACLYVRKMVQDESEETKE-------ANANHCEDDHLKEMEELKEK 735

Query: 460 LKQKEDSSHCCDHLKEVESLKEELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           LK  E +  C  H KE+E LK++L  K++++    K I  +K   +K++ K +
Sbjct: 736 LKAMEFAISCEGHSKEIEELKQKLNAKEHQIQAQDKIIANLKMKLEKKQSKSR 781

BLAST of MS011450 vs. TAIR 10
Match: AT3G50100.1 (small RNA degrading nuclease 1 )

HSP 1 Score: 409.1 bits (1050), Expect = 5.3e-114
Identity = 210/405 (51.85%), Postives = 288/405 (71.11%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           VL E+V+L Q R +RG  G+WKEFL+ +D K   + SDPS+RS++ L+ FL TF K++DL
Sbjct: 12  VLDELVKLLQSRDLRGENGNWKEFLHVYD-KNADSPSDPSRRSHEDLVQFLTTFKKKEDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
               ++L+C+     ++ + Q + D+++PEQ LVRLT+EHP Y L+YSF  Y + W V+ 
Sbjct: 72  ----QLLKCHANHLLIENLKQESQDEDTPEQMLVRLTVEHPSYSLDYSFKPYSEDWFVSD 131

Query: 121 FSQK-SKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              K  K ++S +M A+DCEMVLCEDGTE LVRV +VDRDL+V +DE VKP K + DYRT
Sbjct: 132 VGMKMKKVMESTNMVAVDCEMVLCEDGTEGLVRVGVVDRDLKVILDEFVKPNKPVVDYRT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
           +ITGI+  D++  + S+ DIQ++++  LS GTILVGHSLN DL  LK+DH +VIDT+ +F
Sbjct: 192 DITGITAEDIENASLSVVDIQETLQPFLSTGTILVGHSLNRDLEVLKIDHPKVIDTALVF 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVIVVA 300
           KY N    RRPSLN LCKS+LGYE+RK G PH+C+HDA AAMKL L+ +E +VD  I  +
Sbjct: 252 KYPNTRKLRRPSLNNLCKSILGYEVRKTGVPHDCVHDASAAMKLALAVVEKRVDTTIKPS 311

Query: 301 DEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIFKNPEE 360
            E   ++   KL LH+IP +VPSE+L  V+ G FT+++K +K  QG  Y AFA+F + E+
Sbjct: 312 KE-MLEVEKAKLFLHKIPNNVPSEELEQVLSGKFTLDVKQAKT-QGRYYCAFALFHSSED 371

Query: 361 AQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQND 405
           A QAFE ++G    DS G PQK++  +LSSGS  SIYVRKM Q++
Sbjct: 372 ADQAFEHIDGIEMTDSLGLPQKVVIIKLSSGSRASIYVRKMVQDE 409

BLAST of MS011450 vs. TAIR 10
Match: AT5G67240.2 (small RNA degrading nuclease 3 )

HSP 1 Score: 402.9 bits (1034), Expect = 3.8e-112
Identity = 219/442 (49.55%), Postives = 295/442 (66.74%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFGANMSDPSKRSNDMLIAFLKTFSKEDDL 60
           +LV++V+L QKRG+ G  G WKEFLN +D K G+++SDP++RSND+L+AFL TF K++DL
Sbjct: 12  LLVDLVKLVQKRGLEGENGGWKEFLNVYDKKLGSSLSDPARRSNDVLVAFLLTFKKKEDL 71

Query: 61  MFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVTK 120
             + +V+QC   R  +++  Q T DKE+PEQRLVRLT+ H  YP  Y+FPSY + W VT+
Sbjct: 72  QLIARVMQCGANRELIEKFKQETPDKETPEQRLVRLTITHDDYPGNYTFPSYAEDWYVTE 131

Query: 121 F-SQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              +KSK +KS  M +IDCEMV CEDG++ LVRV  VDRDL+V +D+ VKP K + DY+T
Sbjct: 132 LGKKKSKVIKSTRMLSIDCEMVTCEDGSQALVRVGAVDRDLKVVLDKFVKPDKPVIDYKT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
           +ITG++  DL+  T S+ DIQK +++ LS GTILVGH L+NDL  L++DHARVIDTS+  
Sbjct: 192 DITGVTAEDLERATLSVADIQKKLRRFLSVGTILVGHGLHNDLQVLRIDHARVIDTSY-- 251

Query: 241 KYSNGSIYRRPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDDVI--- 300
                             SVLG E+R +GA HNC+HDA AAMKLVL+ +E     +I   
Sbjct: 252 ------------------SVLGQEVRMDGAAHNCVHDAAAAMKLVLAAVEKGAATLIQPT 311

Query: 301 ---VVAD----EDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYS 360
              +VA+    E   + G  +L LH+IP  VPSE+L GV+ G+FT+ +KP K   G   +
Sbjct: 312 EEMMVAEKRRQEARQEAGKAQLFLHKIPHDVPSEELHGVLSGNFTLVVKPPK--TGGYST 371

Query: 361 AFAIFKNPEEAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGSTVSIYVRKMGQNDFAEKF 420
           A   F +PEEA +AFE VEG   KD SG PQK    +LSSG  VS++VRKM Q+D   + 
Sbjct: 372 AVVDFSSPEEANEAFENVEGDVAKDKSGLPQKKAVLKLSSGLAVSLFVRKMVQDDSPCEI 430

Query: 421 SSKKRDLEVIENLVMSKKQKTD 432
           S+ +R     EN V SK+QKT+
Sbjct: 432 STSER-ARAEENNVSSKRQKTE 430

HSP 2 Score: 44.3 bits (103), Expect = 3.5e-04
Identity = 33/113 (29.20%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 400 MGQNDFAEKFSSKKRDLEVIENLVMSKKQKTDQNIAVETIANSSRC-CDHVEEIERLKQE 459
           + Q   A K SS       +  +V  + ++T +       AN++ C  DH++E+E LK++
Sbjct: 656 LAQKMVAFKLSSGSGACLYVRKMVQDESEETKE-------ANANHCEDDHLKEMEELKEK 715

Query: 460 LKQKEDSSHCCDHLKEVESLKEELRRKDYELSILHKAITIVKKDNKKRKGKKK 512
           LK  E +  C  H KE+E LK++L  K++++    K I  +K   +K++ K +
Sbjct: 716 LKAMEFAISCEGHSKEIEELKQKLNAKEHQIQAQDKIIANLKMKLEKKQSKSR 761

BLAST of MS011450 vs. TAIR 10
Match: AT5G05540.1 (small RNA degrading nuclease 2 )

HSP 1 Score: 373.2 bits (957), Expect = 3.2e-103
Identity = 211/440 (47.95%), Postives = 285/440 (64.77%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFG-ANMSDPSKRSNDMLIAFLKTFSKEDD 60
           VLV++V++AQK G++G  G WKEFL+ +D + G +++SDPSKR  D L+AFL T  K++D
Sbjct: 12  VLVKLVKVAQKLGLKGENGTWKEFLDFYDKQLGSSSLSDPSKRRKDDLVAFLTTLKKKED 71

Query: 61  LMFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVT 120
           L  L K L+ +   N V +  +  S  E+ EQRLVR+TL H +YPL+Y FPS  + W+ T
Sbjct: 72  LQLLAKSLKLD---NDVFEKFKKKSLDETAEQRLVRMTLTHDEYPLDYLFPSNAEDWVRT 131

Query: 121 KFSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              +K        M AIDCEMVLCEDG+E +VRV  VDRDL+V +DE VKP + + DYRT
Sbjct: 132 GLGKKKMEPTKIEMIAIDCEMVLCEDGSEAVVRVAAVDRDLKVILDEFVKPNQPVVDYRT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
            ITG++  DL+  T S+ DIQ+ +   +S  TILVG SLN+DL  LK+DHARVIDTS +F
Sbjct: 192 FITGLTAQDLEKATISVVDIQEKLLMFISEDTILVGQSLNHDLKVLKVDHARVIDTSLVF 251

Query: 241 KYSNGSIYR-----RPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDD 300
           KY+     R     RPSLN LCK +LGYE++KEG PHNC+HDA+AAMKLVL+ L++  + 
Sbjct: 252 KYNYDGTRRPLRLKRPSLNYLCKCILGYEVQKEGVPHNCVHDAEAAMKLVLAILDNGAET 311

Query: 301 VIVVADEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELKPSKKGQGDIYSAFAIF 360
            + ++ E   +    KL LHRIP +VP E+L GV+  D   E+KPSKK     YSA  +F
Sbjct: 312 SVPLSKE-MLEAEKSKLYLHRIPCNVPYEELNGVVSRDIPHEVKPSKKQDRHYYSAIVVF 371

Query: 361 KNPEEAQQAFEQVEGSPDKDSSGRPQKLIKFQLSSGS-TVSIYVRKMGQNDFAEKFSSKK 420
           K+PEEA QAFE + G   KDS G  QK I  + SS    + + VRKM ++D   +  ++ 
Sbjct: 372 KSPEEANQAFENIAGDFGKDSRGLSQKQIFLEPSSSEPRLYVLVRKMVEDDLVGEVIAE- 431

Query: 421 RDLEVIENLVMSKKQKTDQN 434
                 EN   SKK+K + +
Sbjct: 432 ------ENNASSKKRKRENH 440

BLAST of MS011450 vs. TAIR 10
Match: AT5G05540.2 (small RNA degrading nuclease 2 )

HSP 1 Score: 321.2 bits (822), Expect = 1.5e-87
Identity = 173/344 (50.29%), Postives = 233/344 (67.73%), Query Frame = 0

Query: 1   VLVEMVRLAQKRGMRGGEGDWKEFLNSHDPKFG-ANMSDPSKRSNDMLIAFLKTFSKEDD 60
           VLV++V++AQK G++G  G WKEFL+ +D + G +++SDPSKR  D L+AFL T  K++D
Sbjct: 12  VLVKLVKVAQKLGLKGENGTWKEFLDFYDKQLGSSSLSDPSKRRKDDLVAFLTTLKKKED 71

Query: 61  LMFLDKVLQCNEKRNFVKQVSQSTSDKESPEQRLVRLTLEHPQYPLEYSFPSYDKGWIVT 120
           L  L K L+ +   N V +  +  S  E+ EQRLVR+TL H +YPL+Y FPS  + W+ T
Sbjct: 72  LQLLAKSLKLD---NDVFEKFKKKSLDETAEQRLVRMTLTHDEYPLDYLFPSNAEDWVRT 131

Query: 121 KFSQKSKALKSNSMYAIDCEMVLCEDGTENLVRVCMVDRDLQVKIDELVKPRKSIKDYRT 180
              +K        M AIDCEMVLCEDG+E +VRV  VDRDL+V +DE VKP + + DYRT
Sbjct: 132 GLGKKKMEPTKIEMIAIDCEMVLCEDGSEAVVRVAAVDRDLKVILDEFVKPNQPVVDYRT 191

Query: 181 EITGISPGDLDGVTCSLDDIQKSMKKLLSPGTILVGHSLNNDLHALKLDHARVIDTSFIF 240
            ITG++  DL+  T S+ DIQ+ +   +S  TILVG SLN+DL  LK+DHARVIDTS +F
Sbjct: 192 FITGLTAQDLEKATISVVDIQEKLLMFISEDTILVGQSLNHDLKVLKVDHARVIDTSLVF 251

Query: 241 KYSNGSIYR-----RPSLNKLCKSVLGYELRKEGAPHNCLHDAQAAMKLVLSKLESKVDD 300
           KY+     R     RPSLN LCK +LGYE++KEG PHNC+HDA+AAMKLVL+ L++  + 
Sbjct: 252 KYNYDGTRRPLRLKRPSLNYLCKCILGYEVQKEGVPHNCVHDAEAAMKLVLAILDNGAET 311

Query: 301 VIVVADEDTPQIGMEKLLLHRIPIHVPSEDLAGVIRGDFTIELK 339
            + ++ E   +    KL LHRIP +VP E+L GV+  D   E+K
Sbjct: 312 SVPLSKE-MLEAEKSKLYLHRIPCNVPYEELNGVVSRDIPHEVK 351