MS011435 (gene) Bitter gourd (TR) v1

Overview


Name	MS011435
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mitogen-activated protein kinase kinase kinase 17
Location	scaffold239: 1923059 .. 1924093 (+)
RNA-Seq Expression	MS011435
Synteny	MS011435

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACTGGGTTCGAGGACATGAAATTGGACATGGCAGTTTCGCCACCATCAATCTCGCGCTGCCTTCTAAAACTTCTGCTGCTCAATTTCCATCATTAATGGCGGTTAAATCATCTGGAGCTGTCTGCTCTTCCTCGCTTAAGAACGAGAAGCAAGTTTTGGATCTTCTCGGTGATTGCCCACAAATTGTTCGGTGTTTTGGCGAGAGCTGCACCCTCGAGAATGGCGAGCAGTTTTATAATTTGTTTCTGGAGTATGCTAGCGGCGGGAGTTTAGCTGATAGGATACAGACCCGCGGTGGTCGCTTGCCGGAATTTGAGGTTAGGAGGTATACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGGAAAAGGATTTGTTCACTGCGATGTAAAACCCCGGAATATTCTCGTGTTCGATGACGGGGATGTTAAAATTGCCGATTTCGGATTGTCGAAGAAAGCGGGAAAAAACAGAGCAGAAACAGAGCAAGAGAGGAAATTCCAGCTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGGTGAGTCATAACGAGTACGAGTCACCTTGTGATATATGGGCCGTTGGATGCGGGGTGGTGGAGATGTTGACCGGAAAACCCGCCTGGAATTGCCGCCCGGAATCCAACATCTACTCTATATTGCTTCGAATTGGAATCGGAGAGGAGTTGCCGGAATTTCCTAAAGATCTCTCGGAGGAAGGGACGGATTTTCTCGGAAAATGCTTCATCAAGGATCCGACGAAGAGATGGACGGCCGAGATGCTTTTGAATCACCCATTCGTAGCAGACGCCGGAAAGTCTGCTCCACTGACGGCCATGGAGGAAGCATCGACATCGCCGAGGTGCCCTTTCGATTTCGAAGATTGGGCATCGGTTGAGTCGCAGGAGATGGATCAAAGATTTTGGTCGAATTCGTCGTTTTCGTCTTCCCCGACGGAGCGGCTGCTTCAGCTGGTGGACAACGAGGTAGTTGATTGGTCTGTCGCTGACAGTTGGGTCACCGTCAGA

mRNA sequence

ATGGACTGGGTTCGAGGACATGAAATTGGACATGGCAGTTTCGCCACCATCAATCTCGCGCTGCCTTCTAAAACTTCTGCTGCTCAATTTCCATCATTAATGGCGGTTAAATCATCTGGAGCTGTCTGCTCTTCCTCGCTTAAGAACGAGAAGCAAGTTTTGGATCTTCTCGGTGATTGCCCACAAATTGTTCGGTGTTTTGGCGAGAGCTGCACCCTCGAGAATGGCGAGCAGTTTTATAATTTGTTTCTGGAGTATGCTAGCGGCGGGAGTTTAGCTGATAGGATACAGACCCGCGGTGGTCGCTTGCCGGAATTTGAGGTTAGGAGGTATACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGGAAAAGGATTTGTTCACTGCGATGTAAAACCCCGGAATATTCTCGTGTTCGATGACGGGGATGTTAAAATTGCCGATTTCGGATTGTCGAAGAAAGCGGGAAAAAACAGAGCAGAAACAGAGCAAGAGAGGAAATTCCAGCTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGGTGAGTCATAACGAGTACGAGTCACCTTGTGATATATGGGCCGTTGGATGCGGGGTGGTGGAGATGTTGACCGGAAAACCCGCCTGGAATTGCCGCCCGGAATCCAACATCTACTCTATATTGCTTCGAATTGGAATCGGAGAGGAGTTGCCGGAATTTCCTAAAGATCTCTCGGAGGAAGGGACGGATTTTCTCGGAAAATGCTTCATCAAGGATCCGACGAAGAGATGGACGGCCGAGATGCTTTTGAATCACCCATTCGTAGCAGACGCCGGAAAGTCTGCTCCACTGACGGCCATGGAGGAAGCATCGACATCGCCGAGGTGCCCTTTCGATTTCGAAGATTGGGCATCGGTTGAGTCGCAGGAGATGGATCAAAGATTTTGGTCGAATTCGTCGTTTTCGTCTTCCCCGACGGAGCGGCTGCTTCAGCTGGTGGACAACGAGGTAGTTGATTGGTCTGTCGCTGACAGTTGGGTCACCGTCAGA

Coding sequence (CDS)

ATGGACTGGGTTCGAGGACATGAAATTGGACATGGCAGTTTCGCCACCATCAATCTCGCGCTGCCTTCTAAAACTTCTGCTGCTCAATTTCCATCATTAATGGCGGTTAAATCATCTGGAGCTGTCTGCTCTTCCTCGCTTAAGAACGAGAAGCAAGTTTTGGATCTTCTCGGTGATTGCCCACAAATTGTTCGGTGTTTTGGCGAGAGCTGCACCCTCGAGAATGGCGAGCAGTTTTATAATTTGTTTCTGGAGTATGCTAGCGGCGGGAGTTTAGCTGATAGGATACAGACCCGCGGTGGTCGCTTGCCGGAATTTGAGGTTAGGAGGTATACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGGAAAAGGATTTGTTCACTGCGATGTAAAACCCCGGAATATTCTCGTGTTCGATGACGGGGATGTTAAAATTGCCGATTTCGGATTGTCGAAGAAAGCGGGAAAAAACAGAGCAGAAACAGAGCAAGAGAGGAAATTCCAGCTGAGAGGCTCTCCTCTGTACATGTCGCCGGAATCGGTGAGTCATAACGAGTACGAGTCACCTTGTGATATATGGGCCGTTGGATGCGGGGTGGTGGAGATGTTGACCGGAAAACCCGCCTGGAATTGCCGCCCGGAATCCAACATCTACTCTATATTGCTTCGAATTGGAATCGGAGAGGAGTTGCCGGAATTTCCTAAAGATCTCTCGGAGGAAGGGACGGATTTTCTCGGAAAATGCTTCATCAAGGATCCGACGAAGAGATGGACGGCCGAGATGCTTTTGAATCACCCATTCGTAGCAGACGCCGGAAAGTCTGCTCCACTGACGGCCATGGAGGAAGCATCGACATCGCCGAGGTGCCCTTTCGATTTCGAAGATTGGGCATCGGTTGAGTCGCAGGAGATGGATCAAAGATTTTGGTCGAATTCGTCGTTTTCGTCTTCCCCGACGGAGCGGCTGCTTCAGCTGGTGGACAACGAGGTAGTTGATTGGTCTGTCGCTGACAGTTGGGTCACCGTCAGA

Protein sequence

MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDCPQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLRYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSPESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWASVESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR

Homology

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BLAST of MS011435 vs. NCBI nr
Match: XP_022132904.1 (mitogen-activated protein kinase kinase kinase 17 [Momordica charantia])

HSP 1 Score: 714.9 bits (1844), Expect = 3.3e-202
Identity = 343/345 (99.42%), Postives = 343/345 (99.42%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC
Sbjct: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR
Sbjct: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIHGKGFVHCDVKP NILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP
Sbjct: 121 YIHGKGFVHCDVKPGNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS
Sbjct: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWAS 300
           EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWAS
Sbjct: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWAS 300

Query: 301 VESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           VESQEMDQRFWSNSSFSSSPTERLLQLVDNE VDWSVADSWVTVR
Sbjct: 301 VESQEMDQRFWSNSSFSSSPTERLLQLVDNEAVDWSVADSWVTVR 345

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BLAST of MS011435 vs. NCBI nr
Match: XP_038883122.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 603.2 bits (1554), Expect = 1.4e-168
Identity = 294/356 (82.58%), Postives = 314/356 (88.20%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKT-SAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLG- 60
           M+WVRG EIGHGSFATIN A PSKT S  QFP LMAVKSSG VCS+SLKNEKQVLDLLG 
Sbjct: 1   MEWVRGDEIGHGSFATINFATPSKTYSGHQFPPLMAVKSSGVVCSASLKNEKQVLDLLGD 60

Query: 61  DCPQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEG 120
           DCPQI+RCFGESC+ ENGE+ YNLFLEYASGGSLADRIQ+RGGRLPEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGESCSYENGEEIYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAET-EQERKFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVFDDGD KIADFGLSKKAGK RAET E+  +FQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKKRAETGEEAGRFQLRGSPLY 180

Query: 181 MSPESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPK 240
           MSPESV  NEYESPCDIWAVGC VVEMLTGKPAWNCRPESNIYS+LL+IGIGEELPE PK
Sbjct: 181 MSPESVKDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNIYSLLLKIGIGEELPEIPK 240

Query: 241 DLSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFED 300
           DLSEEG DFLGKC +KDP KRWTA+MLL HPFVAD+G++ PLTA++E STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVADSGRTVPLTAVDEPSTSPRCPFDFED 300

Query: 301 WASVESQEMDQRF------WSNSSFS--SSPTERLLQLVDNEVVDWSVADSWVTVR 346
           WAS+ SQE DQRF      W NSS S  SSP ERLLQLVDN  VDWSV ++WVTVR
Sbjct: 301 WASIHSQEADQRFEEESNCWLNSSCSWLSSPRERLLQLVDNGPVDWSVTNNWVTVR 356

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BLAST of MS011435 vs. NCBI nr
Match: XP_023517683.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo] >XP_023522778.1 mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 579.7 bits (1493), Expect = 1.7e-161
Identity = 281/354 (79.38%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           MDW+RGHEIG GSFATINLA+PS TS AQFP LMAVKSS AV S+SLKNEK+VLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSTAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQI+RCFGESC++E G++ YNLFLEYASGGSLADRIQ+ GG LPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIH KGFVHCDVKPRNILVF DGD KIADFGLSKKAGKNRAET +E KFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESV+ NEYESPCDIWAVGC VVEMLTGKPAWN   ESNIYS+LL+IGIGEELP+ PK +S
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSA-PLTAMEEASTSPRCPFDFEDWA 300
           EEG DFLGKC +KDP KRWTAEMLLNHPF+AD+G S+ PLT  +E STSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SVESQEMDQRF------WSNSS--FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           S+ SQE+DQ+F      W +SS  +S SPTERLLQLVDN  VDW + DSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

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BLAST of MS011435 vs. NCBI nr
Match: KAG7027055.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 578.2 bits (1489), Expect = 4.8e-161
Identity = 280/354 (79.10%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           MDW+RGHEIG GSFATINLA+PS TS AQFP LMAVKSS AV S+SLKNEK+VLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQI+RCFGESC++E G++ YNLFLEYASGGSLADRIQ+ GG LPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIH KGFVHCDVKPRNILVF DG+ KIADFGLSKKAGKNRAET +E KFQLRGSPLYMSP
Sbjct: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESV+ NEYESPCDIWAVGC VVEMLTGKPAWN   ESNIYS+LL+IGIGEELP+ PK +S
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSA-PLTAMEEASTSPRCPFDFEDWA 300
           EEG DFLGKC +KDP KRWTAEMLLNHPF+AD+G S+ PLT  +E STSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SVESQEMDQRF------WSNSS--FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           S+ SQE+DQ+F      W +SS  +S SPTERLLQLVDN  VDW + DSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

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BLAST of MS011435 vs. NCBI nr
Match: KAG6595031.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.8 bits (1488), Expect = 6.3e-161
Identity = 280/354 (79.10%), Postives = 308/354 (87.01%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           MDW+RGHEIG GSFATINLA+PS TS AQFP LMAVKSS AV S+SLKNEK+VLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQI+RCFGESC++E G++ YNLFLEYASGGSLADRIQ+ GG LPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIH KGFVHCDVKPRNILVF DG+ KIADFGLSKKAGKNRAET +E KFQLRGSPLYMSP
Sbjct: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESV+ NEYESPCDIWAVGC VVEMLTGKPAWN   ESNIYS+LL+IGIGEELP+ PK +S
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSA-PLTAMEEASTSPRCPFDFEDWA 300
           EEG DFLGKC +KDP KRWTAEMLLNHPF+AD+G S+ PLT  +E STSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SVESQEMDQRF------WSNSS--FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           S+ SQE+DQ+F      W +SS  +S SPTERLLQLVDN  VDW + DSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

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BLAST of MS011435 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.8e-102
Identity = 188/353 (53.26%), Postives = 247/353 (69.97%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           M+WVRG  IG G+F+T++ A  S+ S   FP+L+AVKS+ A  ++SL NEK VLD LGDC
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNS-GDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGR-LPEFEVRRYTRTILEGL 120
           P+I+RC+GE  T+ENGE+ +NL LEYAS GSLA  ++  GG  LPE  VRR+T ++L GL
Sbjct: 61  PEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMS 180
           R+IH KGF HCD+K  NIL+F+DG VKIADFGL+ +   +   T   +  ++RG+PLYM+
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDL--TALRKSVEIRGTPLYMA 180

Query: 181 PESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDL 240
           PE V+ NEY S  D+WA+GC VVEM +GK AW+ +  S+  S+L+RIG+G+ELP+ P+ L
Sbjct: 181 PECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEML 240

Query: 241 SEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVA-----DAGKSAPLTAMEEAST--SPRCP 300
           SEEG DFL KCF+KDP KRWTAEMLLNH FV      D  ++  +   +E     SP+CP
Sbjct: 241 SEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCP 300

Query: 301 FDFEDWASVESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           F+F+DW   +S  +D    SN SF  SP ERL  LV   + DWSV  SW+TVR
Sbjct: 301 FEFDDW---DSFTLD----SNPSF-DSPVERLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of MS011435 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.2e-55
Identity = 120/310 (38.71%), Postives = 165/310 (53.23%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           M+W RG  +G GS AT+  A    +       ++AVKSS    S  L+ E ++L  L   
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSD-----EILAVKSSEVHRSEFLQREAKILSSLSS- 60

Query: 61  PQIVRCFGESCTLE-NGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGL 120
           P ++   G     E NG   YNL +EYA  G+L D     GGR+ E  V +YTR IL+GL
Sbjct: 61  PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMS 180
            YIH KG VHCDVK  N+++ + G+ KIADFG +K+        +   +  + G+P +M+
Sbjct: 121 EYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKR-------VDPVFESPVMGTPAFMA 180

Query: 181 PESVSHNEYESPCDIWAVGCGVVEMLTGKPAW-NCRPESNIYSILLRIGIGEELPEFPKD 240
           PE     +     DIWAVGC ++EM+TG P W       +  S+L R+G   E PE P  
Sbjct: 181 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 240

Query: 241 LSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDW 300
           L+EE  DFL KC  ++  +RWTA  LLNHPF+       P+      S SP    D   W
Sbjct: 241 LAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQTFW 297

Query: 301 ASVESQEMDQ 309
            SVE +E ++
Sbjct: 301 RSVEEEEEEE 297

[Close]

BLAST of MS011435 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.6e-53
Identity = 135/356 (37.92%), Postives = 189/356 (53.09%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           M+W RG  +G GS AT++ A     +  +    +AVKS+    S  L+ E ++L  L + 
Sbjct: 1   MNWTRGKTLGRGSTATVSAA-----TCHESGETLAVKSAEFHRSEFLQREAKILSSL-NS 60

Query: 61  PQIVRCFGESCTLE----NGE-QFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTI 120
           P ++   G   T E    NGE   Y+L +EYA  G+L D     GG + E  V +YTR I
Sbjct: 61  PYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQI 120

Query: 121 LEGLRYIH-GKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGS 180
           L GL YIH  KG  HCD+K  N+LV ++G+ KIADFG +K         E E    +RG+
Sbjct: 121 LLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAK-------WVEPEITEPVRGT 180

Query: 181 PLYMSPESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPE 240
           P +M+PE+          DIWAVGC V+EM+TG   W     ++  S+L R+G   ELPE
Sbjct: 181 PAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPE 240

Query: 241 FPKDLSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFD 300
            P  L+E+  DFLGKC  K+ T+RWTA  LLNHPF+ +     P       + SP    D
Sbjct: 241 LPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVN---KEPELVTGLVTNSPTSVTD 300

Query: 301 FEDWASVESQEMDQRFWSNSSFSSSPTERL--LQLVDNEVVD--WSV-ADSWVTVR 346
              W SVE +  + R   +S +     ER+  L  + + VV+  W +  + W+TVR
Sbjct: 301 QMFWRSVEEEVSEDR---SSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

[Close]

BLAST of MS011435 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.0e-40
Identity = 96/278 (34.53%), Postives = 146/278 (52.52%), Query Frame = 0


Query: 3   WVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC-- 62
           W +G  +G G+F  + L   S+         + V S        LK   Q ++LL     
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCH 273

Query: 63  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 122
           P IV+ +G     E  E+  +++LEY SGGS+   ++  G    E  ++ YTR IL GL 
Sbjct: 274 PNIVQYYGS----ELSEETLSVYLEYVSGGSIHKLLKDYGS-FTEPVIQNYTRQILAGLA 333

Query: 123 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 182
           Y+HG+  VH D+K  NILV  +G++K+ADFG++K        T        +GSP +M+P
Sbjct: 334 YLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHV------TAFSTMLSFKGSPYWMAP 393

Query: 183 ESV-SHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDL 242
           E V S N Y    DIW++GC ++EM T KP W+   +    + + +IG  ++ PE P  L
Sbjct: 394 EVVMSQNGYTHAVDIWSLGCTILEMATSKPPWS---QFEGVAAIFKIGNSKDTPEIPDHL 453

Query: 243 SEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSA 278
           S +  +F+  C  ++PT R TA  LL HPF+ +  + A
Sbjct: 454 SNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVA 477

[Close]

BLAST of MS011435 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.7e-39
Identity = 100/281 (35.59%), Postives = 145/281 (51.60%), Query Frame = 0


Query: 3   WVRGHEIGHGSFATINLALPSKTS---AAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGD 62
           W +G  IG G+F ++ +A  S+T    A +   L       A C   L+ E ++L  L  
Sbjct: 346 WKKGKLIGRGTFGSVYVASNSETGALCAMKEVELFPDDPKSAECIKQLEQEIKLLSNL-Q 405

Query: 63  CPQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGL 122
            P IV+ FG     E  E  + ++LEY   GS+   I+   G + E  VR +TR IL GL
Sbjct: 406 HPNIVQYFGS----ETVEDRFFIYLEYVHPGSINKYIRDHCGTMTESVVRNFTRHILSGL 465

Query: 123 RYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMS 182
            Y+H K  VH D+K  N+LV   G VK+ADFG++K     RA+        L+GSP +M+
Sbjct: 466 AYLHNKKTVHRDIKGANLLVDASGVVKLADFGMAKHLTGQRAD------LSLKGSPYWMA 525

Query: 183 PESV-------SHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEEL 242
           PE +       S+ +     DIW++GC ++EM TGKP W     S          +  + 
Sbjct: 526 PELMQAVMQKDSNPDLAFAVDIWSLGCTIIEMFTGKPPW-----SEFEGAAAMFKVMRDS 585

Query: 243 PEFPKDLSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADA 274
           P  P+ +S EG DFL  CF ++P +R TA MLL H F+ ++
Sbjct: 586 PPIPESMSPEGKDFLRLCFQRNPAERPTASMLLEHRFLKNS 610

[Close]

BLAST of MS011435 vs. ExPASy TrEMBL
Match: A0A6J1BUE3 (mitogen-activated protein kinase kinase kinase 17 OS=Momordica charantia OX=3673 GN=LOC111005631 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.6e-202
Identity = 343/345 (99.42%), Postives = 343/345 (99.42%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC
Sbjct: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR
Sbjct: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIHGKGFVHCDVKP NILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP
Sbjct: 121 YIHGKGFVHCDVKPGNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS
Sbjct: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWAS 300
           EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWAS
Sbjct: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWAS 300

Query: 301 VESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           VESQEMDQRFWSNSSFSSSPTERLLQLVDNE VDWSVADSWVTVR
Sbjct: 301 VESQEMDQRFWSNSSFSSSPTERLLQLVDNEAVDWSVADSWVTVR 345

[Close]

BLAST of MS011435 vs. ExPASy TrEMBL
Match: A0A6J1HG32 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111463177 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 2.0e-160
Identity = 279/354 (78.81%), Postives = 307/354 (86.72%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           MDW+RGHEIG GSFATINLA+PS TS AQFP LMAVKSS AV S+SLKNEK+VLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQI+RCFGESC++E G++ YNLFLEYASGGSLADRIQ+ GG LPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIH KGFVHCDVKPRNILVF DG+ KIADFGLSKKAGKNRAET +E KFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESV+ NEYESPCDIWAVGC VVEMLTGKPAWN   ESNIYS+LL+IGIGEELP+ PK +S
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSA-PLTAMEEASTSPRCPFDFEDWA 300
           EEG DFLGKC +KDP KRWTAEMLLNHPF+AD+G S+ PLT  +E STSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SVESQEMDQRF------WSNSS--FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           S+ SQE+DQ+F      W +SS  +S SPTERLLQLVD   VDW + DSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDKGPVDWVITDSWVTVR 354

[Close]

BLAST of MS011435 vs. ExPASy TrEMBL
Match: A0A5D3CM36 (Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004750 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.4e-160
Identity = 278/356 (78.09%), Postives = 304/356 (85.39%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLG-D 60
           MDWVRGHEIGHGSFATINLA PS  S  +FP LMAVKSS  VCSSSLKNEKQ+LDLLG D
Sbjct: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60

Query: 61  CPQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGL 120
           CPQI+RCFGESC++ENGE+ YNLFLEYA+GGSLADRIQ+ GGRLPEFEVRRYTRTILEGL
Sbjct: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAET-EQERKFQLRGSPLYM 180
           RYIHGKGFVHCDVKPRNILVF DGD KIADFGLSKKAGKNR ET E+  +FQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180

Query: 181 SPESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKD 240
           SPES++ NEYESPCDIWAVGC VVEM TGKPAWNCRPESN++++L++IGIGEELPE PKD
Sbjct: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 LSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDW 300
           LSEEG DFL KC +KDP KRWTA+MLL HPFVAD+G+  PL  M+E STSPRCPFDFEDW
Sbjct: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDW 300

Query: 301 ASVESQEMDQR------FWSNSS---FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           AS+ SQE D R       W NSS    SSSP ERLLQLV+   VDWSV D+WV VR
Sbjct: 301 ASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

[Close]

BLAST of MS011435 vs. ExPASy TrEMBL
Match: A0A1S3B1I0 (mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN=LOC103484781 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.4e-160
Identity = 278/356 (78.09%), Postives = 304/356 (85.39%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLG-D 60
           MDWVRGHEIGHGSFATINLA PS  S  +FP LMAVKSS  VCSSSLKNEKQ+LDLLG D
Sbjct: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60

Query: 61  CPQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGL 120
           CPQI+RCFGESC++ENGE+ YNLFLEYA+GGSLADRIQ+ GGRLPEFEVRRYTRTILEGL
Sbjct: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAET-EQERKFQLRGSPLYM 180
           RYIHGKGFVHCDVKPRNILVF DGD KIADFGLSKKAGKNR ET E+  +FQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180

Query: 181 SPESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKD 240
           SPES++ NEYESPCDIWAVGC VVEM TGKPAWNCRPESN++++L++IGIGEELPE PKD
Sbjct: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 LSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDW 300
           LSEEG DFL KC +KDP KRWTA+MLL HPFVAD+G+  PL  M+E STSPRCPFDFEDW
Sbjct: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFEDW 300

Query: 301 ASVESQEMDQR------FWSNSS---FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           AS+ SQE D R       W NSS    SSSP ERLLQLV+   VDWSV D+WV VR
Sbjct: 301 ASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

[Close]

BLAST of MS011435 vs. ExPASy TrEMBL
Match: A0A6J1KQ57 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111497247 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.4e-159
Identity = 277/354 (78.25%), Postives = 305/354 (86.16%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           M+W+RGHEIG GSFATINLA+PS TS AQFP LMAVKSS AV S+SLKNEK+VLDLLGDC
Sbjct: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           PQI+RCFGESC++E G++ YNLFLEYASGGSLADRIQ+ GG LPEFEVRR+TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           YIH KGFVHCDVKPRNILVF DGD KIADFGLSKKAGKNRAET +E KFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDLS 240
           ESV+ +EYESPCDIWAVGC VVEMLTGKPAWN   E NIYS+LL+IGIGEELP+ PK +S
Sbjct: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSA-PLTAMEEASTSPRCPFDFEDWA 300
           EEG DFLGKC +KDP KRWTAEMLLNHPF+AD+G S+ PLT   E  TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300

Query: 301 SVESQEMDQRF------WSNSS--FSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           S+ S EMDQ+F      W +SS  +S SPTERLLQLVDN  VDW + DSWVTVR
Sbjct: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

[Close]

BLAST of MS011435 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.5e-112
Identity = 200/348 (57.47%), Postives = 254/348 (72.99%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           M+W+R   IGHGSF+T++LA  S +S+  FPSLMAVKSSG VCS++L+NE+ VLD LGDC
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
            +IVRCFGE  T+ENGE+ YNLFLEYASGGSLADRI++ G  LPEFEVRR+TR+I++GL 
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           +IHG GF HCD+K  N+LVF DGDVKI+DFGL+K+          E   ++RG+PLYM+P
Sbjct: 121 HIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKR-------RSGEVCVEIRGTPLYMAP 180

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPE---SNIYSILLRIGIGEELPEFPK 240
           ESV+H E+ESP DIWA+GC VVEM +GK AW C  +   +N+ S+L+RIG G+E+P  P 
Sbjct: 181 ESVNHGEFESPADIWALGCSVVEMSSGKTAW-CLEDGVMNNVMSLLVRIGSGDEVPRIPV 240

Query: 241 DLSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFED 300
           +LSEEG DF+ KCF+K+  +RWTAEMLL+HPF+A   +S      E  S SPR PFDF  
Sbjct: 241 ELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESG--EEDEACSVSPRNPFDFPG 300

Query: 301 WASVESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           W SV+S   D   +   S   SP ER+  LV  +V DWSV+  WV VR
Sbjct: 301 WNSVQSPVNDSVMF--GSLVGSPEERISGLVSEKVPDWSVSCDWVNVR 336

[Close]

BLAST of MS011435 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 375.9 bits (964), Expect = 3.4e-104
Identity = 191/356 (53.65%), Postives = 243/356 (68.26%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLG-D 60
           M+W+RG  IG+G+F+T++LA  S   + +FP LMAVKS+ +  ++SL NEK VLD LG D
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  CPQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGR-LPEFEVRRYTRTILEG 120
           C +IVRCFGE  T+ENGE+ +NLFLEYAS GSL   ++   G  +PE  VRR+T ++L G
Sbjct: 61  CNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYM 180
           LR+IH  GF HCD+K  NIL+F DG VKIADFGL+K+ G     T      Q+RG+PLYM
Sbjct: 121 LRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGD---LTALNYGVQIRGTPLYM 180

Query: 181 SPESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKD 240
           +PESV+ NEY S  D+WA+GC VVEM +GK AW+ +  SN  S+LLRIG+G+E+P  P++
Sbjct: 181 APESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEE 240

Query: 241 LSEEGTDFLGKCFIKDPTKRWTAEMLLNHPFV-ADAGKSAPL--------TAMEEASTSP 300
           LSE+G DFL KCF+KDP KRWTAEMLLNHPFV  D      +           E+ STSP
Sbjct: 241 LSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSP 300

Query: 301 RCPFDFEDWASVESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           RCPF+F DW SV          S S    SP ER+  LV + + DWSV +SWVTVR
Sbjct: 301 RCPFEFPDWVSVS---------SGSQTIDSPDERVASLVTDMIPDWSVTNSWVTVR 344

[Close]

BLAST of MS011435 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 374.0 bits (959), Expect = 1.3e-103
Identity = 188/353 (53.26%), Postives = 247/353 (69.97%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           M+WVRG  IG G+F+T++ A  S+ S   FP+L+AVKS+ A  ++SL NEK VLD LGDC
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNS-GDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 60

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGR-LPEFEVRRYTRTILEGL 120
           P+I+RC+GE  T+ENGE+ +NL LEYAS GSLA  ++  GG  LPE  VRR+T ++L GL
Sbjct: 61  PEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RYIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMS 180
           R+IH KGF HCD+K  NIL+F+DG VKIADFGL+ +   +   T   +  ++RG+PLYM+
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDL--TALRKSVEIRGTPLYMA 180

Query: 181 PESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESNIYSILLRIGIGEELPEFPKDL 240
           PE V+ NEY S  D+WA+GC VVEM +GK AW+ +  S+  S+L+RIG+G+ELP+ P+ L
Sbjct: 181 PECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEML 240

Query: 241 SEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVA-----DAGKSAPLTAMEEAST--SPRCP 300
           SEEG DFL KCF+KDP KRWTAEMLLNH FV      D  ++  +   +E     SP+CP
Sbjct: 241 SEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCP 300

Query: 301 FDFEDWASVESQEMDQRFWSNSSFSSSPTERLLQLVDNEVVDWSVADSWVTVR 346
           F+F+DW   +S  +D    SN SF  SP ERL  LV   + DWSV  SW+TVR
Sbjct: 301 FEFDDW---DSFTLD----SNPSF-DSPVERLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of MS011435 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 132/358 (36.87%), Postives = 193/358 (53.91%), Query Frame = 0


Query: 1   MDWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDC 60
           ++W RG  IG GS AT+++A+ S         L AVKS+    SS L+ E+ +L  L   
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSS------GELFAVKSADLSSSSLLQKEQSILSTLSS- 62

Query: 61  PQIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLR 120
           P +V+  G   T E+    YN+ +EY SGG+L D I+  GG+LPE E+R YTR IL GL 
Sbjct: 63  PHMVKYIGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLV 122

Query: 121 YIHGKGFVHCDVKPRNILVFDDGDVKIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 180
           Y+H +G VHCD+K  N+LV ++G +KIAD G +K   K+          +  G+P +M+P
Sbjct: 123 YLHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSVDKS----------EFSGTPAFMAP 182

Query: 181 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESN-IYSILLRIGIGEELPEFPKDL 240
           E     E   P D+WA+GC ++EM+TG   W   PE N + + + +IG   E P  P  +
Sbjct: 183 EVARGEEQRFPADVWALGCTMIEMMTGSSPW---PELNDVVAAMYKIGFSGESPAIPAWI 242

Query: 241 SEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWA 300
           S++  DFL  C  +D  +RWT E LL HPF+ D  +S     ++  ++SP    D   W 
Sbjct: 243 SDKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKTSSPSTVLDQRFWD 302

Query: 301 SVESQ-------EMDQRFWSNSSFSSSPTERLLQLVDNE---VVDWSVAD--SWVTVR 346
           S ES        + +  F   S    SP +R+ +L  +E   ++DW   D   W+ VR
Sbjct: 303 SCESSKSHLVSIDHEDPFAEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

[Close]

BLAST of MS011435 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 229.9 bits (585), Expect = 3.0e-60
Identity = 137/360 (38.06%), Postives = 197/360 (54.72%), Query Frame = 0


Query: 2   DWVRGHEIGHGSFATINLALPSKTSAAQFPSLMAVKSSGAVCSSSLKNEKQVLDLLGDCP 61
           +W+RG  IG GS AT++L +   T++  F    AVKS+    S+ L+ E+ +L  L   P
Sbjct: 5   NWIRGPIIGRGSTATVSLGI---TNSGDF---FAVKSAEFSSSAFLQREQSILSKLSS-P 64

Query: 62  QIVRCFGESCTLENGEQFYNLFLEYASGGSLADRIQTRGGRLPEFEVRRYTRTILEGLRY 121
            IV+  G + T EN +  YNL +EY SGGSL D I+  GG+LPE  +R YTR IL+GL Y
Sbjct: 65  YIVKYIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMY 124

Query: 122 IHGKGFVHCDVKPRNILVFDDGDV-KIADFGLSKKAGKNRAETEQERKFQLRGSPLYMSP 181
           +H +G VHCDVK +N+++   G++ KI D G +K         E+    +  G+P +MSP
Sbjct: 125 LHDQGIVHCDVKSQNVMI--GGEIAKIVDLGCAK-------TVEENENLEFSGTPAFMSP 184

Query: 182 ESVSHNEYESPCDIWAVGCGVVEMLTGKPAWNCRPESN-IYSILLRIGIGEELPEFPKDL 241
           E     E   P D+WA+GC V+EM TG   W   PE N + + + +IG   E P  P  L
Sbjct: 185 EVARGEEQSFPADVWALGCTVIEMATGSSPW---PELNDVVAAIYKIGFTGESPVIPVWL 244

Query: 242 SEEGTDFLGKCFIKDPTKRWTAEMLLNHPFVADAGKSAPLTAMEEASTSPRCPFDFEDWA 301
           SE+G DFL KC  KDP +RWT E LL HPF+ +    +  T     S+SP    D   W 
Sbjct: 245 SEKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWD 304

Query: 302 SVES-------QEMDQRFWSNSSF----SSSPTERLLQLVDNE---VVDWSVADSWVTVR 346
             E+       ++ +  F ++++F     S P +R+ +L  +E     DW   + W+ VR
Sbjct: 305 LCETSRSRFIKEDHEDPFANSTNFLWDDDSLPGDRIKKLAGDESSGEPDWE-TNGWIEVR 344

The following BLAST results are available for this feature:

BLAST of MS011435 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132904.1	3.3e-202	99.42	mitogen-activated protein kinase kinase kinase 17 [Momordica charantia]	[more]
XP_038883122.1	1.4e-168	82.58	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_023517683.1	1.7e-161	79.38	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe...	[more]
KAG7027055.1	4.8e-161	79.10	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
KAG6595031.1	6.3e-161	79.10	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]

BLAST of MS011435 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	1.8e-102	53.26	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	2.2e-55	38.71	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	1.6e-53	37.92	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
F4HRJ4	2.0e-40	34.53	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]
Q9C5H5	1.7e-39	35.59	Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of MS011435 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUE3	1.6e-202	99.42	mitogen-activated protein kinase kinase kinase 17 OS=Momordica charantia OX=3673...	[more]
A0A6J1HG32	2.0e-160	78.81	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A5D3CM36	4.4e-160	78.09	Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuw...	[more]
A0A1S3B1I0	4.4e-160	78.09	mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN...	[more]
A0A6J1KQ57	6.4e-159	78.25	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36...	[more]

BLAST of MS011435 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	1.5e-112	57.47	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	3.4e-104	53.65	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	1.3e-103	53.26	mitogen-activated protein kinase kinase kinase 20	[more]
AT4G26890.1	1.1e-62	36.87	mitogen-activated protein kinase kinase kinase 16	[more]
AT5G55090.1	3.0e-60	38.06	mitogen-activated protein kinase kinase kinase 15	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..270 e-value: 1.7E-67 score: 240.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 5..270 e-value: 3.1E-55 score: 187.4
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 3..270 score: 39.947643
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..93 e-value: 6.7E-9 score: 37.9
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 94..322 e-value: 2.3E-54 score: 186.4
None	No IPR available	PIRSR	PIRSR037993-2	PIRSR037993-2	coord: 92..277 e-value: 2.8E-12 score: 44.0
None	No IPR available	PANTHER	PTHR48011:SF18	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20	coord: 1..318
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..318
None	No IPR available	CDD	cd06606	STKc_MAPKKK	coord: 2..270 e-value: 3.77237E-116 score: 334.874
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 9..37
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 127..139
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..321

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011435.1	MS011435.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity