MS011391 (gene) Bitter gourd (TR) v1

BLAST of MS011391 vs. NCBI nr
Match: XP_022133332.1 (tRNase Z TRZ1 [Momordica charantia])

HSP 1 Score: 585.5 bits (1508), Expect = 2.6e-163
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA
Sbjct: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           IGQNFLFISHGHMDHIGGLPLYVATRGLY MEPPTIILPKSIKTDVENLLEVHRRLDHSE
Sbjct: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYNMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL
Sbjct: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY
Sbjct: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQ IAALPPPLAGRVFALTEGF
Sbjct: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQTIAALPPPLAGRVFALTEGF 296

BLAST of MS011391 vs. NCBI nr
Match: XP_022979026.1 (tRNase Z TRZ1 [Cucurbita maxima])

HSP 1 Score: 542.3 bits (1396), Expect = 2.5e-150
Identity = 269/296 (90.88%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           ME GTGSSSKS DSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           I QNFL ISHGHMDHIGGLPLYVATRGLYKMEPPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGL+VGEE SLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGL GNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTI++PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHL EIINHA+KFKNKA+L+IHFSARYTTEEIQQA+AALPPPLAGRV ALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

BLAST of MS011391 vs. NCBI nr
Match: KAG6604210.1 (tRNase Z TRZ1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.3 bits (1396), Expect = 2.5e-150
Identity = 269/296 (90.88%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           ME GTGSSSKS DSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           I QNFL ISHGHMDHIGGLPLYVATRGLYKMEPPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGL+VGEE SLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGL GNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTI++PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHL EIINHA+KFKNKA+L+IHFSARYTTEEIQQA+AALPPPLAGRV ALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

BLAST of MS011391 vs. NCBI nr
Match: XP_022950835.1 (tRNase Z TRZ1 [Cucurbita moschata])

HSP 1 Score: 540.8 bits (1392), Expect = 7.3e-150
Identity = 268/296 (90.54%), Postives = 279/296 (94.26%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           ME GTGSSSKS DSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           I QNFL ISHGHMDHIGGLPLYVATRGLYKMEPPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGL+VGEE SLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGL GNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTI++PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHL EIINHA+KFKNKA+L+IHFSARYTTEEIQQA+A LPPPLAGRV ALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVATLPPPLAGRVSALTEGF 296

BLAST of MS011391 vs. NCBI nr
Match: XP_038882012.1 (tRNase Z TRZ1 isoform X1 [Benincasa hispida])

HSP 1 Score: 540.4 bits (1391), Expect = 9.6e-150
Identity = 267/296 (90.20%), Postives = 281/296 (94.93%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           M  GTG SSKS DSDPME+ KGMQIEGYPIKGLSIGGHETCVIFPTL+LAFDIGRCPQKA
Sbjct: 27  MAAGTGCSSKSLDSDPMESCKGMQIEGYPIKGLSIGGHETCVIFPTLSLAFDIGRCPQKA 86

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           I Q+FLFISHGHMDHIGGLPLYVATRGLYKM+PPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 87  ISQDFLFISHGHMDHIGGLPLYVATRGLYKMKPPTIIVPKSIKNDVENLLEVHRRLDQSE 146

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGL+VGEELSLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGLSGNEIKKL
Sbjct: 147 LRCNLIGLEVGEELSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLSGNEIKKL 206

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSG E+TYTI+EPEVAFTGDTTSDFIVDENNID+LRAKILVMESTFLEN+VTVEHAREY
Sbjct: 207 RLSGAEITYTITEPEVAFTGDTTSDFIVDENNIDILRAKILVMESTFLENRVTVEHAREY 266

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHL EIINHA+KFKNKA+LLIHFSARYTTEEIQQAIA +PPPLAGRV AL EGF
Sbjct: 267 GHTHLFEIINHAEKFKNKAILLIHFSARYTTEEIQQAIATIPPPLAGRVSALMEGF 322

BLAST of MS011391 vs. ExPASy Swiss-Prot
Match: Q8LGU7 (tRNase Z TRZ1 OS=Arabidopsis thaliana OX=3702 GN=TRZ1 PE=1 SV=3)

HSP 1 Score: 448.7 bits (1153), Expect = 5.0e-125
Identity = 209/280 (74.64%), Postives = 250/280 (89.29%), Query Frame = 0

Query: 17  MEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAIGQNFLFISHGHMDHI 76
           ME KK MQIEGYPI+GLSIGGHETC+IFP+L +AFDIGRCP +AI Q+FLFISH HMDHI
Sbjct: 1   MEKKKAMQIEGYPIEGLSIGGHETCIIFPSLRIAFDIGRCPHRAISQDFLFISHSHMDHI 60

Query: 77  GGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSELRCNLIGLDVGEELSL 136
           GGLP+YVATRGLYKM+PPTII+P SIK  VE+L EVHR+LD SEL+ NL+GLD+GEE  +
Sbjct: 61  GGLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSELKHNLVGLDIGEEFII 120

Query: 137 RRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKLRLSGVEVTYTISEPEV 196
           R+DLK +AFKT+HVIQSQGY+VYS K KLKKEY+GLSGNEIK L++SGVE+T +I  PEV
Sbjct: 121 RKDLKVKAFKTFHVIQSQGYVVYSTKYKLKKEYIGLSGNEIKNLKVSGVEITDSIITPEV 180

Query: 197 AFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREYGHTHLLEIINHADKFK 256
           AFTGDTTSDF+VDE N D L+AK+LVMESTFL++ V+VEHAR+YGH H+ EI+NHA+KF+
Sbjct: 181 AFTGDTTSDFVVDETNADALKAKVLVMESTFLDDSVSVEHARDYGHIHISEIVNHAEKFE 240

Query: 257 NKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           NKA+LLIHFSARYT +EI+ A++ALPPPL GRVFALT+GF
Sbjct: 241 NKAILLIHFSARYTVKEIEDAVSALPPPLEGRVFALTQGF 280

BLAST of MS011391 vs. ExPASy Swiss-Prot
Match: Q8L633 (tRNase Z TRZ2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TRZ2 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.8e-90
Identity = 154/277 (55.60%), Postives = 206/277 (74.37%), Query Frame = 0

Query: 20  KKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAIGQNFLFISHGHMDHIGGL 79
           +KG+++E Y I+G+S+GGHETCVI P L   FDIGRCP +AI Q FLFI+H H+DHIGGL
Sbjct: 73  RKGVELESYAIEGISVGGHETCVIVPELKCVFDIGRCPSRAIQQKFLFITHAHLDHIGGL 132

Query: 80  PLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSELRCNLIGLDVGEELSLRRD 139
           P+YVA+RGLY +EPP I +P SIK DVE LLE+HR +   EL   LI L VGE   LR D
Sbjct: 133 PMYVASRGLYNLEPPKIFVPPSIKEDVEKLLEIHRTMGQVELNVELIPLAVGETYELRND 192

Query: 140 LKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKLRLSGVEVTYTISEPEVAFT 199
           +  R F T+HVI SQGY++YSV++KL+K+Y  L G +I+K++ SGVE+T TI  PE+AFT
Sbjct: 193 IVVRPFATHHVIPSQGYVIYSVRKKLQKQYAHLKGKQIEKIKKSGVEITDTILSPEIAFT 252

Query: 200 GDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREYGHTHLLEIINHADKFKNKA 259
           GDTTS++++D  N D LRAK+L+ E+TFL+   + EHA+  GHTH+ +II +A   ++K 
Sbjct: 253 GDTTSEYMLDPRNADALRAKVLITEATFLDESFSTEHAQALGHTHISQIIENAKWIRSKT 312

Query: 260 VLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           VLL HFS+RY  EEI++A+  L   ++ +V  LTEGF
Sbjct: 313 VLLTHFSSRYHVEEIREAVLKLQSKVSAKVIPLTEGF 349

BLAST of MS011391 vs. ExPASy Swiss-Prot
Match: P60193 (Nuclear ribonuclease Z (Fragment) OS=Triticum aestivum OX=4565 GN=ELAC PE=1 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.0e-70
Identity = 120/175 (68.57%), Postives = 151/175 (86.29%), Query Frame = 0

Query: 18  EAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAIGQNFLFISHGHMDHIG 77
           +AK  ++IEGYP++G+SIGGHETCVIFPTL+LAFDIGRCPQ+A+ Q+FLFISH H+DHIG
Sbjct: 2   KAKPRLEIEGYPVEGISIGGHETCVIFPTLSLAFDIGRCPQRAVAQDFLFISHAHLDHIG 61

Query: 78  GLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSELRCNLIGLDVGEELSLR 137
           GLP+YVATRGLY++ PPTI +PK ++  VE L +VHR +D SEL   L+ LD+GEE  LR
Sbjct: 62  GLPMYVATRGLYRLRPPTIFVPKYLRELVERLFDVHRAMDQSELNHTLVPLDIGEEYELR 121

Query: 138 RDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKLRLSGVEVTYTIS 193
           RDLK RAFKTYH I SQGY++YSVKQKLK++YLGL G+EIK+L+LSGVE+T T++
Sbjct: 122 RDLKVRAFKTYHTIPSQGYVIYSVKQKLKQDYLGLPGSEIKRLKLSGVEITNTVT 176

BLAST of MS011391 vs. ExPASy Swiss-Prot
Match: Q973F1 (Ribonuclease Z OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=rnz PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.4e-10
Identity = 63/221 (28.51%), Postives = 106/221 (47.96%), Query Frame = 0

Query: 68  ISHGHMDHIGGLPLYVATRGLYKMEPPTIIL-PKSIKTDVENLLEVHRRLDHSELRCNLI 127
           I+H H DH+ GLP  + T G+Y  +   +++ PK +K  +E++ +      + E++    
Sbjct: 58  ITHMHGDHVLGLPGMIETMGMYSRKESLLLMGPKELKEFLEDIFKKTYFYPNFEIQI--- 117

Query: 128 GLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKLRL---- 187
            +D  E+     ++K   F+T H I+SQGYL +  K +LK +   L    IK  R+    
Sbjct: 118 -IDKYED----ENIKISTFETCHTIESQGYL-FEEKDRLKIDIDKLRKEGIKDWRIIRML 177

Query: 188 ---SGVEVTYTISEPE----------VAFTGDTTSDFIVDENNIDVLR-AKILVMESTFL 247
                VE+   +  PE          +A+TGDT       E  I+ ++   +L+ +STF+
Sbjct: 178 KEGKRVEINGKVLLPEDYLIVKKGIRIAYTGDTGP----CEKVINAVKDVDLLIHDSTFI 237

Query: 248 ENKVTVEHAREYGHTHLLEIINHADKFKNKAVLLIHFSARY 270
           + K     A +YGH++  +    A K   K + L H S RY
Sbjct: 238 DEK----EAYKYGHSNSYDAAYVALKANVKRLALFHISPRY 261

BLAST of MS011391 vs. ExPASy Swiss-Prot
Match: A4YHP1 (Ribonuclease Z OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) OX=399549 GN=rnz PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.4e-10
Identity = 73/240 (30.42%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 64  NFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIIL-PKSIKTDVENLLEVHRRLDHSELR 123
           N + I+H H DH+ GLP  + T G+Y  +    IL P+ +K   + L E   R   S   
Sbjct: 54  NVIAITHLHADHVLGLPSLIQTMGMYDRKERLYILGPEGLK---DLLTETFERTYFSP-- 113

Query: 124 CNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSV------KQKLKKEYLGLSGNE 183
            N     V    S  + ++ R F+T HV+ SQGYLV          ++L++E  G++   
Sbjct: 114 -NFPIEFVSSYES--QGIRVRPFRTCHVVPSQGYLVEEKDTANLDAERLRRE--GVTDWR 173

Query: 184 IKKLRLSGVEVTY--TISEPE----------VAFTGDTTSDFIVDENNIDVLRAKILVME 243
           + ++   G EV +   + +PE          +A+TGDT     V  +   V    +L+ +
Sbjct: 174 VMRMLKEGKEVPWGDRVLKPEDYLIVKRGIRIAYTGDTRPCETVINS---VKGVDLLLHD 233

Query: 244 STFLENKVTVEHAREYGHTHLLEIINHADKFKNKAVLLIHFSARY--TTEEIQQAIAALP 283
           STF +       A EYGH+   E    A + + K + LIH SARY  T+E ++QA    P
Sbjct: 234 STFEQG----IDASEYGHSTSTEAATVAREAEVKRLALIHISARYRDTSEMLKQARRIFP 276

BLAST of MS011391 vs. ExPASy TrEMBL
Match: A0A6J1BWD5 (tRNase Z TRZ1 OS=Momordica charantia OX=3673 GN=LOC111005940 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.3e-163
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA
Sbjct: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           IGQNFLFISHGHMDHIGGLPLYVATRGLY MEPPTIILPKSIKTDVENLLEVHRRLDHSE
Sbjct: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYNMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL
Sbjct: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY
Sbjct: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQ IAALPPPLAGRVFALTEGF
Sbjct: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQTIAALPPPLAGRVFALTEGF 296

BLAST of MS011391 vs. ExPASy TrEMBL
Match: A0A6J1IPL7 (tRNase Z TRZ1 OS=Cucurbita maxima OX=3661 GN=LOC111478792 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.2e-150
Identity = 269/296 (90.88%), Postives = 280/296 (94.59%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           ME GTGSSSKS DSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERMKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           I QNFL ISHGHMDHIGGLPLYVATRGLYKMEPPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGL+VGEE SLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGL GNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTI++PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHL EIINHA+KFKNKA+L+IHFSARYTTEEIQQA+AALPPPLAGRV ALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVAALPPPLAGRVSALTEGF 296

BLAST of MS011391 vs. ExPASy TrEMBL
Match: A0A6J1GGV3 (tRNase Z TRZ1 OS=Cucurbita moschata OX=3662 GN=LOC111453818 PE=4 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.5e-150
Identity = 268/296 (90.54%), Postives = 279/296 (94.26%), Query Frame = 0

Query: 1   METGTGSSSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKA 60
           ME GTGSSSKS DSD  E  KGMQIEGYPIKGLSIGGHETC+IFPTLNLAFDIGRCPQKA
Sbjct: 1   MEAGTGSSSKSLDSDLKERTKGMQIEGYPIKGLSIGGHETCIIFPTLNLAFDIGRCPQKA 60

Query: 61  IGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSE 120
           I QNFL ISHGHMDHIGGLPLYVATRGLYKMEPPTII+PKSIK DVENLLEVHRRLD SE
Sbjct: 61  ISQNFLLISHGHMDHIGGLPLYVATRGLYKMEPPTIIVPKSIKNDVENLLEVHRRLDQSE 120

Query: 121 LRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKL 180
           LRCNLIGL+VGEE SLRRDLK RAFKTYHVI SQGYL+YSVKQKLKKEYLGL GNEIKKL
Sbjct: 121 LRCNLIGLEVGEEFSLRRDLKVRAFKTYHVIDSQGYLLYSVKQKLKKEYLGLPGNEIKKL 180

Query: 181 RLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREY 240
           RLSGVEVTYTI++PEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHAREY
Sbjct: 181 RLSGVEVTYTITDPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHAREY 240

Query: 241 GHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           GHTHL EIINHA+KFKNKA+L+IHFSARYTTEEIQQA+A LPPPLAGRV ALTEGF
Sbjct: 241 GHTHLFEIINHAEKFKNKAILMIHFSARYTTEEIQQAVATLPPPLAGRVSALTEGF 296

BLAST of MS011391 vs. ExPASy TrEMBL
Match: A0A5A7V6W7 (Nuclear ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00700 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 6.9e-146
Identity = 261/297 (87.88%), Postives = 278/297 (93.60%), Query Frame = 0

Query: 1   METGTGS-SSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           M  GT + SSK+ DS+PME  KG+QI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AIGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHS 120
           AI Q+FLFISHGHMDHIGGLPLYVATRGLYKM PPTI++PKSIK DVENLLEVHRRLD S
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKK 180
           ELRCNLIGL+VGEELSLRRDLK R F+TYHVI SQGYL+YSVKQKLKK+YLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHARE 240
           LRLSGVE+TYTI+EPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           YGHTHL EIINHA KFKNKA+LLIHFSARYTTEEIQ+AI+ LPPPLAGRV ALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPPLAGRVSALTEGF 297

BLAST of MS011391 vs. ExPASy TrEMBL
Match: A0A1S3C5G3 (nuclear ribonuclease Z OS=Cucumis melo OX=3656 GN=LOC103497261 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 260/297 (87.54%), Postives = 277/297 (93.27%), Query Frame = 0

Query: 1   METGTGS-SSKSSDSDPMEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60
           M  GT + SSK+ DS+PME  KG+QI GYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK
Sbjct: 1   MAAGTANFSSKNLDSNPMEMSKGIQIAGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQK 60

Query: 61  AIGQNFLFISHGHMDHIGGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHS 120
           AI Q+FLFISHGHMDHIGGLPLYVATRGLYKM PPTI++PKSIK DVENLLEVHRRLD S
Sbjct: 61  AISQDFLFISHGHMDHIGGLPLYVATRGLYKMRPPTIVVPKSIKNDVENLLEVHRRLDQS 120

Query: 121 ELRCNLIGLDVGEELSLRRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKK 180
           ELRCNLIGL+VGEELSLRRDLK R F+TYHVI SQGYL+YSVKQKLKK+YLGLSGNEIKK
Sbjct: 121 ELRCNLIGLEVGEELSLRRDLKVRVFRTYHVIDSQGYLLYSVKQKLKKDYLGLSGNEIKK 180

Query: 181 LRLSGVEVTYTISEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHARE 240
           LRLSGVE+TYTI+EPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLEN+VTVEHARE
Sbjct: 181 LRLSGVEITYTITEPEVAFTGDTTSDFIVDENNIDVLRAKILVMESTFLENRVTVEHARE 240

Query: 241 YGHTHLLEIINHADKFKNKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           YGHTHL EIINHA KFKNKA+LLIHFSARYTTEEIQ+AI+ LPP LAGRV ALTEGF
Sbjct: 241 YGHTHLFEIINHAKKFKNKAILLIHFSARYTTEEIQEAISTLPPLLAGRVSALTEGF 297

BLAST of MS011391 vs. TAIR 10
Match: AT1G74700.1 (tRNAse Z1 )

HSP 1 Score: 448.7 bits (1153), Expect = 3.5e-126
Identity = 209/280 (74.64%), Postives = 250/280 (89.29%), Query Frame = 0

Query: 17  MEAKKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAIGQNFLFISHGHMDHI 76
           ME KK MQIEGYPI+GLSIGGHETC+IFP+L +AFDIGRCP +AI Q+FLFISH HMDHI
Sbjct: 1   MEKKKAMQIEGYPIEGLSIGGHETCIIFPSLRIAFDIGRCPHRAISQDFLFISHSHMDHI 60

Query: 77  GGLPLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSELRCNLIGLDVGEELSL 136
           GGLP+YVATRGLYKM+PPTII+P SIK  VE+L EVHR+LD SEL+ NL+GLD+GEE  +
Sbjct: 61  GGLPMYVATRGLYKMKPPTIIVPASIKETVESLFEVHRKLDSSELKHNLVGLDIGEEFII 120

Query: 137 RRDLKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKLRLSGVEVTYTISEPEV 196
           R+DLK +AFKT+HVIQSQGY+VYS K KLKKEY+GLSGNEIK L++SGVE+T +I  PEV
Sbjct: 121 RKDLKVKAFKTFHVIQSQGYVVYSTKYKLKKEYIGLSGNEIKNLKVSGVEITDSIITPEV 180

Query: 197 AFTGDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREYGHTHLLEIINHADKFK 256
           AFTGDTTSDF+VDE N D L+AK+LVMESTFL++ V+VEHAR+YGH H+ EI+NHA+KF+
Sbjct: 181 AFTGDTTSDFVVDETNADALKAKVLVMESTFLDDSVSVEHARDYGHIHISEIVNHAEKFE 240

Query: 257 NKAVLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           NKA+LLIHFSARYT +EI+ A++ALPPPL GRVFALT+GF
Sbjct: 241 NKAILLIHFSARYTVKEIEDAVSALPPPLEGRVFALTQGF 280

BLAST of MS011391 vs. TAIR 10
Match: AT2G04530.1 (Metallo-hydrolase/oxidoreductase superfamily protein )

HSP 1 Score: 332.0 bits (850), Expect = 4.8e-91
Identity = 154/277 (55.60%), Postives = 206/277 (74.37%), Query Frame = 0

Query: 20  KKGMQIEGYPIKGLSIGGHETCVIFPTLNLAFDIGRCPQKAIGQNFLFISHGHMDHIGGL 79
           +KG+++E Y I+G+S+GGHETCVI P L   FDIGRCP +AI Q FLFI+H H+DHIGGL
Sbjct: 73  RKGVELESYAIEGISVGGHETCVIVPELKCVFDIGRCPSRAIQQKFLFITHAHLDHIGGL 132

Query: 80  PLYVATRGLYKMEPPTIILPKSIKTDVENLLEVHRRLDHSELRCNLIGLDVGEELSLRRD 139
           P+YVA+RGLY +EPP I +P SIK DVE LLE+HR +   EL   LI L VGE   LR D
Sbjct: 133 PMYVASRGLYNLEPPKIFVPPSIKEDVEKLLEIHRTMGQVELNVELIPLAVGETYELRND 192

Query: 140 LKARAFKTYHVIQSQGYLVYSVKQKLKKEYLGLSGNEIKKLRLSGVEVTYTISEPEVAFT 199
           +  R F T+HVI SQGY++YSV++KL+K+Y  L G +I+K++ SGVE+T TI  PE+AFT
Sbjct: 193 IVVRPFATHHVIPSQGYVIYSVRKKLQKQYAHLKGKQIEKIKKSGVEITDTILSPEIAFT 252

Query: 200 GDTTSDFIVDENNIDVLRAKILVMESTFLENKVTVEHAREYGHTHLLEIINHADKFKNKA 259
           GDTTS++++D  N D LRAK+L+ E+TFL+   + EHA+  GHTH+ +II +A   ++K 
Sbjct: 253 GDTTSEYMLDPRNADALRAKVLITEATFLDESFSTEHAQALGHTHISQIIENAKWIRSKT 312

Query: 260 VLLIHFSARYTTEEIQQAIAALPPPLAGRVFALTEGF 297
           VLL HFS+RY  EEI++A+  L   ++ +V  LTEGF
Sbjct: 313 VLLTHFSSRYHVEEIREAVLKLQSKVSAKVIPLTEGF 349