MS011346 (gene) Bitter gourd (TR) v1

Overview


Name	MS011346
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	WRKY domain-containing protein
Location	scaffold239: 1201177 .. 1202653 (+)
RNA-Seq Expression	MS011346
Synteny	MS011346

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTCCCCTCAAAACCCTAGTTTCCTCAATCACCATCACTTTCAGGACTCCTCCTCCTCATTCATGGATTTCTTCGATTTCTCGGGTTACCCGCCACCCGATTTCATTGTCGAAGAAGCCGAAACGACGACGTTGCCGGAGGCAAGAATCGGCAGGCGGAGCATACCCATGACGACGGAAGCAACTTTCACAGACAATAATACCATGCAAGTGAAATTAAAAATTATAGCTTTATCTTGCATATAGTTAATAATTTTAATAAACCCCATGCATGTATTTATACATATATATCATATTGTTAAAGTTTTCATAATTAATATATATTTGTGTGCGTTTTTGCAGAGAGGAGTGGTGCGAGACGAAGGGTGTGAAGAGAAAAAAAGAGGGAGGAGGTGGGTGCAGCAATAGGGTTGCATTTAGAACCAAGTCGGAGCTGGAGATTTTGGATGATGGCTTCAAGTGGAGAAAGTATGGCAAGAAATCTGTCAAGGATAGCCCTAATCCAAGGTAAATAATACACATCCTAGTTAAATTAGTTAAAGACATTTATTTTTACTAAAATATCAAAACTTTAAATCATAAGTATGTTGAGTTAAAAAAAAAAAATTACATATTTCTAGGAAGAGAGAGATGCGGCTAGATACACTTGAATTAAGAACGTGTGGTAAAGGTTTATATAGTGGTGGAAAGTTGAATGTGTCATGAAGATGATGATTGGAGGGAGAAGTGGAAACTCCAATATTTATTTATGATATTATTCTATTAATTATAGTCTTCTTTTATATCACTTCACTTACCTTTAAGACCAAACTAATATGCTTTACTTTTTGTTCTTGTTTTACTTGTATGGATAATGTGGAGTTTAATGTATGGATAATAACAACTTAAAAGATATATAATAGTAATTTAATTAAGTTTTGCCACCATGCCTAAATTAAATAAAATATGATGTAAAATCTTAAAAACTACTCTAAAAAAATATCCATCTTTTATTTCGTTGTGTATGTGTTATAATTCCTAGAAACATAACTATGTGGAAAGTGAAAACTGTACATTTGAAAGAGAGAAAACTTTGGTTCATATATATTTCAAGATATAAGTGTAAAGTAGTATAAGAAGATTAAAAATTATATCGATTTGAACTATATGGCAGTGTCGATCCGTTTAATATGTATATATATATATGGTGTTGTAAATGATAAATATTTGTGAAGTTTATTGATGATTTTGATTTTGGATGTAATGTGCAATTGTTAGGAATTACTACAAATGCTCAAGTGGAGGTTGTGGAGTGAAGAAGAGAGTAGAGAGAGACAGGGATGATTCAAGCTATGTTATTACAACATATGAAGGAGTTCACAACCATGAGAGCCCTTTCTTGGTTTATTGCAATGATCCAACATTTCACCCTCATGCCACTTGGCCTTCCACTTTCCACCACTCTTCTCCGCCCTACTCTTCTACTACTACCCTT

mRNA sequence

ATGAACTCCCCTCAAAACCCTAGTTTCCTCAATCACCATCACTTTCAGGACTCCTCCTCCTCATTCATGGATTTCTTCGATTTCTCGGGTTACCCGCCACCCGATTTCATTGTCGAAGAAGCCGAAACGACGACGTTGCCGGAGGCAAGAATCGGCAGGCGGAGCATACCCATGACGACGGAAGCAACTTTCACAGACAATAATACCATGCAAGAGTGGTGCGAGACGAAGGGTGTGAAGAGAAAAAAAGAGGGAGGAGGTGGGTGCAGCAATAGGGTTGCATTTAGAACCAAGTCGGAGCTGGAGATTTTGGATGATGGCTTCAAGTGGAGAAAGTATGGCAAGAAATCTGTCAAGGATAGCCCTAATCCAAGGAATTACTACAAATGCTCAAGTGGAGGTTGTGGAGTGAAGAAGAGAGTAGAGAGAGACAGGGATGATTCAAGCTATGTTATTACAACATATGAAGGAGTTCACAACCATGAGAGCCCTTTCTTGGTTTATTGCAATGATCCAACATTTCACCCTCATGCCACTTGGCCTTCCACTTTCCACCACTCTTCTCCGCCCTACTCTTCTACTACTACCCTT

Coding sequence (CDS)

ATGAACTCCCCTCAAAACCCTAGTTTCCTCAATCACCATCACTTTCAGGACTCCTCCTCCTCATTCATGGATTTCTTCGATTTCTCGGGTTACCCGCCACCCGATTTCATTGTCGAAGAAGCCGAAACGACGACGTTGCCGGAGGCAAGAATCGGCAGGCGGAGCATACCCATGACGACGGAAGCAACTTTCACAGACAATAATACCATGCAAGAGTGGTGCGAGACGAAGGGTGTGAAGAGAAAAAAAGAGGGAGGAGGTGGGTGCAGCAATAGGGTTGCATTTAGAACCAAGTCGGAGCTGGAGATTTTGGATGATGGCTTCAAGTGGAGAAAGTATGGCAAGAAATCTGTCAAGGATAGCCCTAATCCAAGGAATTACTACAAATGCTCAAGTGGAGGTTGTGGAGTGAAGAAGAGAGTAGAGAGAGACAGGGATGATTCAAGCTATGTTATTACAACATATGAAGGAGTTCACAACCATGAGAGCCCTTTCTTGGTTTATTGCAATGATCCAACATTTCACCCTCATGCCACTTGGCCTTCCACTTTCCACCACTCTTCTCCGCCCTACTCTTCTACTACTACCCTT

Protein sequence

MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTTEATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATWPSTFHHSSPPYSSTTTL

Homology

[Close]

BLAST of MS011346 vs. NCBI nr
Match: XP_022133095.1 (probable WRKY transcription factor 51 [Momordica charantia])

HSP 1 Score: 405.2 bits (1040), Expect = 3.2e-109
Identity = 192/197 (97.46%), Postives = 194/197 (98.48%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTT 60
           MNSPQNPS LNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAET TLPEARIGRRSIPMTT
Sbjct: 1   MNSPQNPSCLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETMTLPEARIGRRSIPMTT 60

Query: 61  EATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120
           EATFTDNNT++EWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD
Sbjct: 61  EATFTDNNTIEEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120

Query: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180
           SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW
Sbjct: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180

Query: 181 PSTFHHSSPPYSSTTTL 198
           PSTFH SSPPYSSTTTL
Sbjct: 181 PSTFHRSSPPYSSTTTL 197

[Close]

BLAST of MS011346 vs. NCBI nr
Match: XP_038883139.1 (probable WRKY transcription factor 75 [Benincasa hispida])

HSP 1 Score: 261.2 bits (666), Expect = 7.4e-66
Identity = 139/198 (70.20%), Postives = 152/198 (76.77%), Query Frame = 0


Query: 1   MNSPQNPS-FLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMT 60
           MNSPQN S F ++H FQDSSSS MDF +FSGYP PDF +E  E T++             
Sbjct: 1   MNSPQNLSFFFDNHQFQDSSSSSMDFLNFSGYPTPDFHLEAMEATSI------------- 60

Query: 61  TEATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVK 120
                 DNNT+  WCE KGV+RKKE GGGC+NRVAF TKSELEILDDGFKWRKYGKKSVK
Sbjct: 61  ------DNNTIDGWCEAKGVERKKERGGGCNNRVAFITKSELEILDDGFKWRKYGKKSVK 120

Query: 121 DSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDP-TFHPHA 180
           +SP+ RNYYKCSSGGC VKKRVERDRDDSSYVITTYEGVHNHESPFL+YCNDP  FHPH 
Sbjct: 121 NSPHFRNYYKCSSGGCVVKKRVERDRDDSSYVITTYEGVHNHESPFLMYCNDPILFHPH- 177

Query: 181 TWPSTFHHSSPPYSSTTT 197
             P   + SSPPYSSTTT
Sbjct: 181 -HPLCPNTSSPPYSSTTT 177

[Close]

BLAST of MS011346 vs. NCBI nr
Match: XP_022963170.1 (probable WRKY transcription factor 51 [Cucurbita moschata])

HSP 1 Score: 252.3 bits (643), Expect = 3.5e-63
Identity = 130/199 (65.33%), Postives = 151/199 (75.88%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTT 60
           MNS QNP+      F+DSS+SFMD  DFSGY  PDF + E E+T LPEA IG RS  M  
Sbjct: 3   MNSLQNPT-----DFEDSSTSFMDLLDFSGYLLPDFWL-EPESTVLPEATIGSRSRSMEE 62

Query: 61  EATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120
             +  D  ++ EW E +GVKRK++G GG  ++VAFRTKSELE+L DGFKWRKYGKKS+KD
Sbjct: 63  ATSTMDKTSIDEWHERRGVKRKQKGRGGGCHKVAFRTKSELEVLQDGFKWRKYGKKSIKD 122

Query: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180
           +PNPRNYYKCSS GC VKKRVERDR+DSSYVITTYEG+H+HESPF   CNDP FH H + 
Sbjct: 123 NPNPRNYYKCSSNGCRVKKRVERDREDSSYVITTYEGIHSHESPFTKCCNDPIFHSHGSC 182

Query: 181 PST--FHHSSPPYSSTTTL 198
           P +   HHSSPP SSTTTL
Sbjct: 183 PDSTYLHHSSPPCSSTTTL 195

[Close]

BLAST of MS011346 vs. NCBI nr
Match: XP_023518785.1 (probable WRKY transcription factor 51 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.1 bits (640), Expect = 7.7e-63
Identity = 131/199 (65.83%), Postives = 152/199 (76.38%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTT 60
           MNS QNP+      F+DSSSSFMD  DFSGY  PDF + E ETT  PEA IG R+  M  
Sbjct: 1   MNSLQNPT-----QFEDSSSSFMDLLDFSGYLLPDFWL-EPETTAFPEAIIGGRTRSMEE 60

Query: 61  EATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120
             +  D N++ EW E +GVKRK++G GG  ++VAFRTKSELEIL DGFKWRKYGKKS+K+
Sbjct: 61  ATSTMDKNSIDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKN 120

Query: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180
           +PNPRNYYKCSS GCGVKKRVERDR+DSSYVITTYEG+H+HESPF   CNDP FH H + 
Sbjct: 121 NPNPRNYYKCSSSGCGVKKRVERDREDSSYVITTYEGIHSHESPFSKCCNDPIFHSHGSC 180

Query: 181 PST--FHHSSPPYSSTTTL 198
           P++   H SSPP SSTTTL
Sbjct: 181 PNSTYLHLSSPPCSSTTTL 193

[Close]

BLAST of MS011346 vs. NCBI nr
Match: XP_004141964.1 (probable WRKY transcription factor 51 [Cucumis sativus] >KGN48423.1 hypothetical protein Csa_004416 [Cucumis sativus])

HSP 1 Score: 250.0 bits (637), Expect = 1.7e-62
Identity = 144/205 (70.24%), Postives = 156/205 (76.10%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQ----DSSSSFMDFFDFSGYPPPDFIVEEAETTT--LPEARIGRR 60
           MNS QNP+F   HH Q     SSSS MDF +FSGYP PDF + EAETTT  L EA  G  
Sbjct: 1   MNSLQNPNFFFDHHQQFDQDHSSSSIMDFLNFSGYPLPDFGL-EAETTTFSLSEAETGDG 60

Query: 61  SIPMTTEATFTDNNTMQE-WCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKY 120
           S  M  +AT  DNNT+ + W E KGVKRKK    G +NRVAF TKSELEILDDGFKWRKY
Sbjct: 61  SGSM--KATSIDNNTIDDGWFEGKGVKRKKPRENGRTNRVAFITKSELEILDDGFKWRKY 120

Query: 121 GKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCN-DP 180
           GKKSVK+SP+PRNYYKCSSG CGVKKRVERDRDDSSYVITTYEGVHNHESPFL+YCN   
Sbjct: 121 GKKSVKNSPHPRNYYKCSSGECGVKKRVERDRDDSSYVITTYEGVHNHESPFLMYCNGSK 180

Query: 181 TFHPHATWPSTFHHSSPPYSSTTTL 198
            FHPH   P++   SSPPYSSTTTL
Sbjct: 181 LFHPHPICPNS---SSPPYSSTTTL 199

[Close]

BLAST of MS011346 vs. ExPASy Swiss-Prot
Match: Q93WU9 (Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.3e-32
Identity = 88/190 (46.32%), Postives = 116/190 (61.05%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSS--SSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPM 60
           MN  QNPS  N  +F D +  + FMD  DFS      F ++E     L E  I   +  +
Sbjct: 1   MNISQNPS-PNFTYFSDENFINPFMDNNDFSNL--MFFDIDEGGNNGLIEEEISSPTSIV 60

Query: 61  TTEATFTDNN-------TMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWR 120
           ++E TFT  +       T+ +   T    ++ +      +RVAFRT+S+++++DDGFKWR
Sbjct: 61  SSE-TFTGESGGSGSATTLSKKESTNRGSKESDQTKETGHRVAFRTRSKIDVMDDGFKWR 120

Query: 121 KYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCND 180
           KYGKKSVK++ N RNYYKCSS GC VKKRVERD DD++YVITTYEGVHNHES   VY N+
Sbjct: 121 KYGKKSVKNNINKRNYYKCSSEGCSVKKRVERDGDDAAYVITTYEGVHNHESLSNVYYNE 180

[Close]

BLAST of MS011346 vs. ExPASy Swiss-Prot
Match: Q8VWQ5 (Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.8e-29
Identity = 62/89 (69.66%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 75  ETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGG 134
           + +  K KK+  G    RVAF+T+SE+E+LDDGFKWRKYGKK VK+SP+PRNYYKCS  G
Sbjct: 87  DNQNKKEKKKIKG----RVAFKTRSEVEVLDDGFKWRKYGKKMVKNSPHPRNYYKCSVDG 146

Query: 135 CGVKKRVERDRDDSSYVITTYEGVHNHES 164
           C VKKRVERDRDD S+VITTYEG HNH S
Sbjct: 147 CPVKKRVERDRDDPSFVITTYEGSHNHSS 171

[Close]

BLAST of MS011346 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.1e-23
Identity = 76/197 (38.58%), Postives = 99/197 (50.25%), Query Frame = 0


Query: 2   NSPQNP-SFLN----HHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSI 61
           +S  NP SFL+     H F  SS+S    F F  +P P+     +  T LP  +     +
Sbjct: 86  HSSNNPNSFLDLLRQDHQFASSSNS--SSFSFDAFPLPNNNNNTSFFTDLPLPQAESSEV 145

Query: 62  PMTT------------------------EATFTDNNTMQEWCETKGVK-----RKKEGGG 121
             TT                        E T  D     +  E KG K     +KK    
Sbjct: 146 VNTTPTSPNSTSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKAKKKNQKK 205

Query: 122 GCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDD 165
               R AF TKS+++ LDDG++WRKYG+K+VK+SP PR+YY+C++ GCGVKKRVER  DD
Sbjct: 206 AREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDD 265

[Close]

BLAST of MS011346 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.0e-22
Identity = 51/88 (57.95%), Postives = 66/88 (75.00%), Query Frame = 0


Query: 77  KGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGGCG 136
           KG K+++E       RVAF TKSE++ L+DG++WRKYG+K+VK+SP PR+YY+C++  C 
Sbjct: 114 KGEKKERE------VRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCN 173

Query: 137 VKKRVERDRDDSSYVITTYEGVHNHESP 165
           VKKRVER   D S VITTYEG HNH  P
Sbjct: 174 VKKRVERSFQDPSIVITTYEGKHNHPIP 195

[Close]

BLAST of MS011346 vs. ExPASy Swiss-Prot
Match: Q6VWJ6 (WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.2e-22
Identity = 54/113 (47.79%), Postives = 68/113 (60.18%), Query Frame = 0


Query: 52  GRRSIPMTTEATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWR 111
           G R  P        D  + +   E+ G+     G      RV  +T SE++ILDDG++WR
Sbjct: 310 GGRPYPGDDATEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSEVDILDDGYRWR 369

Query: 112 KYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESP 165
           KYG+K VK +PNPR+YYKC+S GC V+K VER   D   VITTYEG HNHE P
Sbjct: 370 KYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHNHEVP 422

[Close]

BLAST of MS011346 vs. ExPASy TrEMBL
Match: A0A6J1BUC3 (probable WRKY transcription factor 51 OS=Momordica charantia OX=3673 GN=LOC111005775 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.5e-109
Identity = 192/197 (97.46%), Postives = 194/197 (98.48%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTT 60
           MNSPQNPS LNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAET TLPEARIGRRSIPMTT
Sbjct: 1   MNSPQNPSCLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETMTLPEARIGRRSIPMTT 60

Query: 61  EATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120
           EATFTDNNT++EWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD
Sbjct: 61  EATFTDNNTIEEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120

Query: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180
           SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW
Sbjct: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180

Query: 181 PSTFHHSSPPYSSTTTL 198
           PSTFH SSPPYSSTTTL
Sbjct: 181 PSTFHRSSPPYSSTTTL 197

[Close]

BLAST of MS011346 vs. ExPASy TrEMBL
Match: A0A6J1HH83 (probable WRKY transcription factor 51 OS=Cucurbita moschata OX=3662 GN=LOC111463457 PE=4 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.7e-63
Identity = 130/199 (65.33%), Postives = 151/199 (75.88%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTT 60
           MNS QNP+      F+DSS+SFMD  DFSGY  PDF + E E+T LPEA IG RS  M  
Sbjct: 3   MNSLQNPT-----DFEDSSTSFMDLLDFSGYLLPDFWL-EPESTVLPEATIGSRSRSMEE 62

Query: 61  EATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120
             +  D  ++ EW E +GVKRK++G GG  ++VAFRTKSELE+L DGFKWRKYGKKS+KD
Sbjct: 63  ATSTMDKTSIDEWHERRGVKRKQKGRGGGCHKVAFRTKSELEVLQDGFKWRKYGKKSIKD 122

Query: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180
           +PNPRNYYKCSS GC VKKRVERDR+DSSYVITTYEG+H+HESPF   CNDP FH H + 
Sbjct: 123 NPNPRNYYKCSSNGCRVKKRVERDREDSSYVITTYEGIHSHESPFTKCCNDPIFHSHGSC 182

Query: 181 PST--FHHSSPPYSSTTTL 198
           P +   HHSSPP SSTTTL
Sbjct: 183 PDSTYLHHSSPPCSSTTTL 195

[Close]

BLAST of MS011346 vs. ExPASy TrEMBL
Match: A0A0A0KFX2 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486960 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 8.3e-63
Identity = 144/205 (70.24%), Postives = 156/205 (76.10%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQ----DSSSSFMDFFDFSGYPPPDFIVEEAETTT--LPEARIGRR 60
           MNS QNP+F   HH Q     SSSS MDF +FSGYP PDF + EAETTT  L EA  G  
Sbjct: 1   MNSLQNPNFFFDHHQQFDQDHSSSSIMDFLNFSGYPLPDFGL-EAETTTFSLSEAETGDG 60

Query: 61  SIPMTTEATFTDNNTMQE-WCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKY 120
           S  M  +AT  DNNT+ + W E KGVKRKK    G +NRVAF TKSELEILDDGFKWRKY
Sbjct: 61  SGSM--KATSIDNNTIDDGWFEGKGVKRKKPRENGRTNRVAFITKSELEILDDGFKWRKY 120

Query: 121 GKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCN-DP 180
           GKKSVK+SP+PRNYYKCSSG CGVKKRVERDRDDSSYVITTYEGVHNHESPFL+YCN   
Sbjct: 121 GKKSVKNSPHPRNYYKCSSGECGVKKRVERDRDDSSYVITTYEGVHNHESPFLMYCNGSK 180

Query: 181 TFHPHATWPSTFHHSSPPYSSTTTL 198
            FHPH   P++   SSPPYSSTTTL
Sbjct: 181 LFHPHPICPNS---SSPPYSSTTTL 199

[Close]

BLAST of MS011346 vs. ExPASy TrEMBL
Match: A0A1S3AZZ8 (probable WRKY transcription factor 51 OS=Cucumis melo OX=3656 GN=LOC103484689 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 3.2e-62
Identity = 139/203 (68.47%), Postives = 155/203 (76.35%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHH---FQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIP 60
           MNS QNP+F   HH    QDSSSSFMDF +FSGYP PDF + EAETT    +  G     
Sbjct: 1   MNSLQNPTFFFDHHQQLDQDSSSSFMDFLNFSGYPLPDFGL-EAETTMFSLSEAGTGDGS 60

Query: 61  MTTEATFTDNNTMQE-WCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKK 120
            + +AT  DNNT+ + W E KGVKRKKE G GC+++VAF TKSELEILDDG+KWRKYGKK
Sbjct: 61  RSMKATSIDNNTIDDGWFEGKGVKRKKERGNGCNHKVAFITKSELEILDDGYKWRKYGKK 120

Query: 121 SVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTF-H 180
           SVK+SP+PRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFL+Y N     H
Sbjct: 121 SVKNSPHPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLMYSNGSKLCH 180

Query: 181 PHATWPSTFHHSSP-PYSSTTTL 198
           PH   P   + SSP PYSSTTTL
Sbjct: 181 PHPQ-PICPNSSSPDPYSSTTTL 201

[Close]

BLAST of MS011346 vs. ExPASy TrEMBL
Match: A0A6J1KRV9 (probable WRKY transcription factor 51 OS=Cucurbita maxima OX=3661 GN=LOC111497082 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.3e-59
Identity = 125/199 (62.81%), Postives = 146/199 (73.37%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPMTT 60
           MNS QNP+      F+D SSSFMD  DFSGYP PDF + E E   LPEA IG +S  M  
Sbjct: 3   MNSLQNPT-----QFEDLSSSFMDLLDFSGYPLPDFWL-EPEPAVLPEATIGGQSRSMEE 62

Query: 61  EATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120
             +  D N++ EW E + VKR++ G GG  ++VAFRTKSELEIL DGFKWRKYGKKS+K+
Sbjct: 63  ATSRMDKNSIDEWRERRDVKREQNGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKN 122

Query: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCNDPTFHPHATW 180
           +PNPRNYYKC S GC VKKRVERDR+DSSYVITTYEG+H+HESP    CNDP FH H + 
Sbjct: 123 NPNPRNYYKCLSSGCRVKKRVERDREDSSYVITTYEGIHSHESPITKCCNDPIFHSHGSC 182

Query: 181 PST--FHHSSPPYSSTTTL 198
           P++   H SSPP SSTTTL
Sbjct: 183 PNSTYLHLSSPPSSSTTTL 195

[Close]

BLAST of MS011346 vs. TAIR 10
Match: AT5G64810.1 (WRKY DNA-binding protein 51 )

HSP 1 Score: 139.4 bits (350), Expect = 3.0e-33
Identity = 88/190 (46.32%), Postives = 116/190 (61.05%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSS--SSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSIPM 60
           MN  QNPS  N  +F D +  + FMD  DFS      F ++E     L E  I   +  +
Sbjct: 1   MNISQNPS-PNFTYFSDENFINPFMDNNDFSNL--MFFDIDEGGNNGLIEEEISSPTSIV 60

Query: 61  TTEATFTDNN-------TMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWR 120
           ++E TFT  +       T+ +   T    ++ +      +RVAFRT+S+++++DDGFKWR
Sbjct: 61  SSE-TFTGESGGSGSATTLSKKESTNRGSKESDQTKETGHRVAFRTRSKIDVMDDGFKWR 120

Query: 121 KYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPFLVYCND 180
           KYGKKSVK++ N RNYYKCSS GC VKKRVERD DD++YVITTYEGVHNHES   VY N+
Sbjct: 121 KYGKKSVKNNINKRNYYKCSSEGCSVKKRVERDGDDAAYVITTYEGVHNHESLSNVYYNE 180

[Close]

BLAST of MS011346 vs. TAIR 10
Match: AT5G26170.1 (WRKY DNA-binding protein 50 )

HSP 1 Score: 129.0 bits (323), Expect = 4.1e-30
Identity = 62/89 (69.66%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 75  ETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGG 134
           + +  K KK+  G    RVAF+T+SE+E+LDDGFKWRKYGKK VK+SP+PRNYYKCS  G
Sbjct: 87  DNQNKKEKKKIKG----RVAFKTRSEVEVLDDGFKWRKYGKKMVKNSPHPRNYYKCSVDG 146

Query: 135 CGVKKRVERDRDDSSYVITTYEGVHNHES 164
           C VKKRVERDRDD S+VITTYEG HNH S
Sbjct: 147 CPVKKRVERDRDDPSFVITTYEGSHNHSS 171

[Close]

BLAST of MS011346 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-24
Identity = 76/197 (38.58%), Postives = 99/197 (50.25%), Query Frame = 0


Query: 2   NSPQNP-SFLN----HHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETTTLPEARIGRRSI 61
           +S  NP SFL+     H F  SS+S    F F  +P P+     +  T LP  +     +
Sbjct: 86  HSSNNPNSFLDLLRQDHQFASSSNS--SSFSFDAFPLPNNNNNTSFFTDLPLPQAESSEV 145

Query: 62  PMTT------------------------EATFTDNNTMQEWCETKGVK-----RKKEGGG 121
             TT                        E T  D     +  E KG K     +KK    
Sbjct: 146 VNTTPTSPNSTSVSSSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKAKKKNQKK 205

Query: 122 GCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDD 165
               R AF TKS+++ LDDG++WRKYG+K+VK+SP PR+YY+C++ GCGVKKRVER  DD
Sbjct: 206 AREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDD 265

[Close]

BLAST of MS011346 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 106.3 bits (264), Expect = 2.9e-23
Identity = 51/88 (57.95%), Postives = 66/88 (75.00%), Query Frame = 0


Query: 77  KGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGGCG 136
           KG K+++E       RVAF TKSE++ L+DG++WRKYG+K+VK+SP PR+YY+C++  C 
Sbjct: 114 KGEKKERE------VRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCN 173

Query: 137 VKKRVERDRDDSSYVITTYEGVHNHESP 165
           VKKRVER   D S VITTYEG HNH  P
Sbjct: 174 VKKRVERSFQDPSIVITTYEGKHNHPIP 195

[Close]

BLAST of MS011346 vs. TAIR 10
Match: AT2G21900.1 (WRKY DNA-binding protein 59 )

HSP 1 Score: 104.8 bits (260), Expect = 8.3e-23
Identity = 79/215 (36.74%), Postives = 110/215 (51.16%), Query Frame = 0


Query: 1   MNSPQNPSFLNHHHFQDSSSSFMDFFDFSGYPPP-DFIVEE--------------AETTT 60
           MN P NP+         SS+ F +FF F  +    + I+EE              + +  
Sbjct: 1   MNYPSNPN--------PSSTDFTEFFKFDDFDDTFEKIMEEIGREDHSSSPTLSWSSSEK 60

Query: 61  LPEARIGRRSIPMTTEATFTDNNTMQEWCETKGVKRKKEGGGGCSNRVAFRTKSELE--- 120
           L  A I   S   T+ AT   +  + +  E K  KR KE          F+TKS ++   
Sbjct: 61  LVAAEI--TSPLQTSLATSPMSFEIGDKDEIKKRKRHKED----PIIHVFKTKSSIDEKV 120

Query: 121 ILDDGFKWRKYGKKSVKDSPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHE 180
            LDDG+KWRKYGKK +  SP PR+Y+KCSS  C VKK++ERD ++  Y++TTYEG HNH 
Sbjct: 121 ALDDGYKWRKYGKKPITGSPFPRHYHKCSSPDCNVKKKIERDTNNPDYILTTYEGRHNHP 180

Query: 181 SPFLVYCNDPTFHPHA--TW------PSTFHHSSP 190
           SP +VYC+   F  ++   W        +F HS+P
Sbjct: 181 SPSVVYCDSDDFDLNSLNNWSFQTANTYSFSHSAP 201

The following BLAST results are available for this feature:

BLAST of MS011346 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133095.1	3.2e-109	97.46	probable WRKY transcription factor 51 [Momordica charantia]	[more]
XP_038883139.1	7.4e-66	70.20	probable WRKY transcription factor 75 [Benincasa hispida]	[more]
XP_022963170.1	3.5e-63	65.33	probable WRKY transcription factor 51 [Cucurbita moschata]	[more]
XP_023518785.1	7.7e-63	65.83	probable WRKY transcription factor 51 [Cucurbita pepo subsp. pepo]	[more]
XP_004141964.1	1.7e-62	70.24	probable WRKY transcription factor 51 [Cucumis sativus] >KGN48423.1 hypothetical...	[more]

BLAST of MS011346 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WU9	4.3e-32	46.32	Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 ...	[more]
Q8VWQ5	5.8e-29	69.66	Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 ...	[more]
Q9FGZ4	2.1e-23	38.58	Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ...	[more]
Q93WV4	4.0e-22	57.95	WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1	[more]
Q6VWJ6	5.2e-22	47.79	WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1	[more]

BLAST of MS011346 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUC3	1.5e-109	97.46	probable WRKY transcription factor 51 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A6J1HH83	1.7e-63	65.33	probable WRKY transcription factor 51 OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]
A0A0A0KFX2	8.3e-63	70.24	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486960 PE=4 S...	[more]
A0A1S3AZZ8	3.2e-62	68.47	probable WRKY transcription factor 51 OS=Cucumis melo OX=3656 GN=LOC103484689 PE...	[more]
A0A6J1KRV9	3.3e-59	62.81	probable WRKY transcription factor 51 OS=Cucurbita maxima OX=3661 GN=LOC11149708...	[more]

BLAST of MS011346 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64810.1	3.0e-33	46.32	WRKY DNA-binding protein 51	[more]
AT5G26170.1	4.1e-30	69.66	WRKY DNA-binding protein 50	[more]
AT5G49520.1	1.5e-24	38.58	WRKY DNA-binding protein 48	[more]
AT1G29860.1	2.9e-23	57.95	WRKY DNA-binding protein 71	[more]
AT2G21900.1	8.3e-23	36.74	WRKY DNA-binding protein 59	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 104..163 e-value: 3.2E-34 score: 129.7
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 105..162 e-value: 3.8E-23 score: 81.5
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 99..164 score: 31.100372
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 91..157 e-value: 3.5E-31 score: 108.7
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 97..164
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 58..170
None	No IPR available	PANTHER	PTHR31221:SF219	WRKY TRANSCRIPTION FACTOR 50-RELATED	coord: 58..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011346.1	MS011346.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding