MS011276 (gene) Bitter gourd (TR) v1

BLAST of MS011276 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 221.9 bits (564), Expect = 3.1e-54
Identity = 108/122 (88.52%), Postives = 113/122 (92.62%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASL 60
           MDPVAVAPAVQ NDALLNVQKPGGWEPIKNINDPYVQE+GRFAVMEHTH+    +CGAS 
Sbjct: 1   MDPVAVAPAVQQNDALLNVQKPGGWEPIKNINDPYVQELGRFAVMEHTHN----LCGASY 60

Query: 61  EFIRVVSGESQVVAGKKYCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 120
           EFIRVVSGESQVV GKKYCLVIEVKETIIIPSMPSSIK+FKAI+LDK WEKSW LLSFVP
Sbjct: 61  EFIRVVSGESQVVGGKKYCLVIEVKETIIIPSMPSSIKFFKAIMLDKAWEKSWALLSFVP 118

Query: 121 CE 123
           CE
Sbjct: 121 CE 118

BLAST of MS011276 vs. NCBI nr
Match: XP_022132703.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 137.1 bits (344), Expect = 1.0e-28
Identity = 80/126 (63.49%), Postives = 96/126 (76.19%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASL 60
           MD VAVAPA   NDAL +V+KPGGWEPIKNI DPYVQEMGRFAVME      G++  AS+
Sbjct: 32  MDSVAVAPA--QNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLV--ASM 91

Query: 61  EFIRVVSGESQVV-AGKKYCLVIEVK--ETIIIPSMP-SSIKYFKAIVLDKPWEKSWELL 120
           EF+ VVSGE Q+V AGK+Y LV+EVK  E II+   P SSI+ + A+VLDKP +KSW++L
Sbjct: 92  EFVGVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVL 151

Query: 121 SFVPCE 123
           SF P E
Sbjct: 152 SFAPLE 152

BLAST of MS011276 vs. NCBI nr
Match: XP_022132702.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 136.7 bits (343), Expect = 1.3e-28
Identity = 79/126 (62.70%), Postives = 95/126 (75.40%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASL 60
           MD VAVAPA   NDALL+++ PGGWEPIKNI DPYVQEMGRFAVME       +I  AS+
Sbjct: 1   MDSVAVAPA--QNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELI--ASM 60

Query: 61  EFIRVVSGESQVVA-GKKYCLVIEVK--ETIIIPSMP-SSIKYFKAIVLDKPWEKSWELL 120
           EFI VVSGE Q+VA GK+Y LV+EV+  E II+   P SSI+ + A+VLDKP +KSW++L
Sbjct: 61  EFIGVVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVL 120

Query: 121 SFVPCE 123
           SF P E
Sbjct: 121 SFAPLE 122

BLAST of MS011276 vs. NCBI nr
Match: WP_198843152.1 (hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical protein [Aureibaculum sp. A20])

HSP 1 Score: 128.3 bits (321), Expect = 4.7e-26
Identity = 71/125 (56.80%), Postives = 92/125 (73.60%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDAL-LNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGAS 60
           MD   +AP    NDAL  N  KPGGWEPIKNI+DPYVQEMGRF+VMEHTH     + GAS
Sbjct: 5   MDFEVIAPG--QNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTHK----LVGAS 64

Query: 61  LEFIRVVSGESQVVA-GKKYCLVIEVKETIII--PSMPSSIKYFKAIVLDKPWEKSWELL 120
           ++FIRVVSGE++ ++ GK+Y LV+EV E II   P +  S+K++ A VL+KP ++SW+L 
Sbjct: 65  MKFIRVVSGETRPMSEGKEYRLVVEVAEHIITCPPMLSVSVKFYLATVLEKPLDRSWQLE 123

Query: 121 SFVPC 122
           +FV C
Sbjct: 125 AFVSC 123

BLAST of MS011276 vs. NCBI nr
Match: XP_038882290.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 125.2 bits (313), Expect = 3.9e-25
Identity = 73/123 (59.35%), Postives = 91/123 (73.98%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALL-NVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGAS 60
           MD   VAP    NDALL N  +PG WEPIKNI+DPYVQEMGRFAVMEHTH     + GAS
Sbjct: 1   MDYEVVAPG--QNDALLQNRCEPGCWEPIKNIHDPYVQEMGRFAVMEHTHK----LVGAS 60

Query: 61  LEFIRVVSGESQVVA-GKKYCLVIEVKETIII--PSMPSSIKYFKAIVLDKPWEKSWELL 120
           L+FI VVSGE++ ++ GK+Y LV+EV E II   P +  SIK++ A VL+KP ++SW+L+
Sbjct: 61  LKFICVVSGETRPMSQGKEYRLVVEVAEQIITSPPMLSVSIKFYLATVLEKPIDRSWQLI 117

BLAST of MS011276 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.0e-11
Identity = 41/98 (41.84%), Postives = 51/98 (52.04%), Query Frame = 0

Query: 23  GGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLVI 82
           GGW PI N+ DP V E+G FAV E+          + L+F  VVSGE+QVV+G  Y L +
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSE-----SGLKFETVVSGETQVVSGTNYRLKV 88

Query: 83  EVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
              +           K + AIV DKPW K   L SF P
Sbjct: 89  AANDG------DGVSKNYLAIVWDKPWMKFRNLTSFEP 115

BLAST of MS011276 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 2.9e-10
Identity = 37/96 (38.54%), Postives = 53/96 (55.21%), Query Frame = 0

Query: 23  GGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLVI 82
           GGW PI+N+N   VQ++ +FAV EH    +       L++  VV G +QVVAG  Y LVI
Sbjct: 30  GGWRPIENLNSAEVQDVAQFAVSEHNKQAND-----ELQYQSVVRGYTQVVAGTNYRLVI 89

Query: 83  EVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSF 119
             K+  ++ +       ++A+V DKPW     L SF
Sbjct: 90  AAKDGAVVGN-------YEAVVWDKPWMHFRNLTSF 113

BLAST of MS011276 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 2.9e-10
Identity = 34/98 (34.69%), Postives = 52/98 (53.06%), Query Frame = 0

Query: 23  GGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLVI 82
           GGW PI ++ DP++QE+G +AV      +H  +    L F RV SGE QVV+G  Y LV+
Sbjct: 33  GGWSPITDVGDPHIQELGGWAV-----ERHASLSSDGLRFRRVTSGEQQVVSGMNYRLVV 92

Query: 83  EVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
              +         +   + A+V ++ W  + +L SF P
Sbjct: 93  SASDP------AGATASYVAVVYEQSWTNTRQLTSFKP 119

BLAST of MS011276 vs. ExPASy Swiss-Prot
Match: Q5N806 (Cysteine proteinase inhibitor 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0915200 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.4e-10
Identity = 39/103 (37.86%), Postives = 57/103 (55.34%), Query Frame = 0

Query: 20  QKPGGWEPIKNI-NDPYVQEMGRFAVMEHTHSKHGIICG-ASLEFIRVVSGESQVVAGKK 79
           ++ GG   ++++  D  VQE+GRF+V EH   +    CG   LEF RVV+ + QVV+G K
Sbjct: 48  RRVGGRTEVRDVEGDREVQELGRFSVEEHNRRRRSRDCGDVRLEFGRVVAAQRQVVSGLK 107

Query: 80  YCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
           Y L +   E           + F A+V+ KPW +S  LL+F P
Sbjct: 108 YYLRVAAAEEGAAGQNGGEPRVFDAVVVVKPWLESRTLLTFAP 150

BLAST of MS011276 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 6.4e-10
Identity = 36/97 (37.11%), Postives = 52/97 (53.61%), Query Frame = 0

Query: 22  PGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLV 81
           P GW PIKNI+DP++QE+GR+A+ E+      +     L F RV  GE QVV+G  Y L 
Sbjct: 20  PRGWSPIKNIDDPHIQELGRWAITENNR----VSPSDELTFHRVTGGEQQVVSGMNYRLE 79

Query: 82  IEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSF 119
           IE                + A+V ++ W  + +L+SF
Sbjct: 80  IEAAS-----GGGDVTGSYGAVVFEQEWSNTRKLISF 107

BLAST of MS011276 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.5e-54
Identity = 108/122 (88.52%), Postives = 113/122 (92.62%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASL 60
           MDPVAVAPAVQ NDALLNVQKPGGWEPIKNINDPYVQE+GRFAVMEHTH+    +CGAS 
Sbjct: 1   MDPVAVAPAVQQNDALLNVQKPGGWEPIKNINDPYVQELGRFAVMEHTHN----LCGASY 60

Query: 61  EFIRVVSGESQVVAGKKYCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 120
           EFIRVVSGESQVV GKKYCLVIEVKETIIIPSMPSSIK+FKAI+LDK WEKSW LLSFVP
Sbjct: 61  EFIRVVSGESQVVGGKKYCLVIEVKETIIIPSMPSSIKFFKAIMLDKAWEKSWALLSFVP 118

Query: 121 CE 123
           CE
Sbjct: 121 CE 118

BLAST of MS011276 vs. ExPASy TrEMBL
Match: A0A6J1BX23 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005501 PE=4 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.9e-29
Identity = 80/126 (63.49%), Postives = 96/126 (76.19%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASL 60
           MD VAVAPA   NDAL +V+KPGGWEPIKNI DPYVQEMGRFAVME      G++  AS+
Sbjct: 32  MDSVAVAPA--QNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLV--ASM 91

Query: 61  EFIRVVSGESQVV-AGKKYCLVIEVK--ETIIIPSMP-SSIKYFKAIVLDKPWEKSWELL 120
           EF+ VVSGE Q+V AGK+Y LV+EVK  E II+   P SSI+ + A+VLDKP +KSW++L
Sbjct: 92  EFVGVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVL 151

Query: 121 SFVPCE 123
           SF P E
Sbjct: 152 SFAPLE 152

BLAST of MS011276 vs. ExPASy TrEMBL
Match: A0A6J1BUK6 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005500 PE=4 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 6.3e-29
Identity = 79/126 (62.70%), Postives = 95/126 (75.40%), Query Frame = 0

Query: 1   MDPVAVAPAVQHNDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASL 60
           MD VAVAPA   NDALL+++ PGGWEPIKNI DPYVQEMGRFAVME       +I  AS+
Sbjct: 1   MDSVAVAPA--QNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELI--ASM 60

Query: 61  EFIRVVSGESQVVA-GKKYCLVIEVK--ETIIIPSMP-SSIKYFKAIVLDKPWEKSWELL 120
           EFI VVSGE Q+VA GK+Y LV+EV+  E II+   P SSI+ + A+VLDKP +KSW++L
Sbjct: 61  EFIGVVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLDKPSDKSWKVL 120

Query: 121 SFVPCE 123
           SF P E
Sbjct: 121 SFAPLE 122

BLAST of MS011276 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.4e-22
Identity = 61/109 (55.96%), Postives = 76/109 (69.72%), Query Frame = 0

Query: 13  NDALLNVQKPGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQV 72
           NDALL+  K   WEP+ N++D YVQE+GRFAVMEH H     +CG  + FI VVSGES++
Sbjct: 11  NDALLHKPKSCDWEPV-NVHDQYVQELGRFAVMEHVHG----LCGVGMTFICVVSGESKM 70

Query: 73  VA-GKKYCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
           V  GKKY LV+EVKE  ++P    +IK + A VLDK   KSWELL+F P
Sbjct: 71  VCEGKKYSLVVEVKEVPVLPHACPAIKLYVADVLDKQCAKSWELLAFKP 114

BLAST of MS011276 vs. ExPASy TrEMBL
Match: A0A6J1FCN5 (cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111442890 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 8.6e-18
Identity = 56/118 (47.46%), Postives = 74/118 (62.71%), Query Frame = 0

Query: 6   VAPAVQHNDALLNVQK---PGGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEF 65
           V  A   N+A+   +K    G W+PI++I DPYVQEMGRFAVMEH + +     G +L+F
Sbjct: 5   VEAAPGQNEAMFQDKKGIEVGQWKPIEDIKDPYVQEMGRFAVMEHDNMR----IGENLKF 64

Query: 66  IRVVSGESQVVAGKKYCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
           I V SGE QVV G  Y L+I+    + I S+      + AIV DKPWEK+W+L+ FVP
Sbjct: 65  ICVQSGEKQVVEGMNYRLLIKASNHLGISSL------YMAIVYDKPWEKTWKLIEFVP 112

BLAST of MS011276 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 1.4e-12
Identity = 41/98 (41.84%), Postives = 51/98 (52.04%), Query Frame = 0

Query: 23  GGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLVI 82
           GGW PI N+ DP V E+G FAV E+          + L+F  VVSGE+QVV+G  Y L +
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSE-----SGLKFETVVSGETQVVSGTNYRLKV 88

Query: 83  EVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
              +           K + AIV DKPW K   L SF P
Sbjct: 89  AANDG------DGVSKNYLAIVWDKPWMKFRNLTSFEP 115

BLAST of MS011276 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 3.2e-09
Identity = 35/96 (36.46%), Postives = 52/96 (54.17%), Query Frame = 0

Query: 23  GGWEPIKNINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLVI 82
           G  +PIKN++DP V  + ++A+ EH            L F++VV G +QVV+G KY L I
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHNKESK-----EKLVFVKVVEGTTQVVSGTKYDLKI 89

Query: 83  EVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSF 119
             K+          IK ++A+V++K W  S  L SF
Sbjct: 90  AAKDG------GGKIKNYEAVVVEKLWLHSKSLESF 114

BLAST of MS011276 vs. TAIR 10
Match: AT2G31980.1 (PHYTOCYSTATIN 2 )

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-06
Identity = 36/111 (32.43%), Postives = 53/111 (47.75%), Query Frame = 0

Query: 23  GGWEPIKNI-NDPYVQEMGRFAVMEHTH------------SKHGIICGASLEFIRVVSGE 82
           GG   + NI  +  +Q++GR+ V +               +K        L+F RVVS +
Sbjct: 37  GGKSGVPNIRTNREIQQLGRYCVEQFNQQAQNEQGNIGSIAKTDTAISNPLQFSRVVSAQ 96

Query: 83  SQVVAGKKYCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSFVP 121
            QVVAG KY L IEV +         S + F ++V+ +PW  S +LL F P
Sbjct: 97  KQVVAGLKYYLRIEVTQP------NGSTRMFDSVVVIQPWLHSKQLLGFTP 141

BLAST of MS011276 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 50.1 bits (118), Expect = 1.5e-06
Identity = 31/89 (34.83%), Postives = 50/89 (56.18%), Query Frame = 0

Query: 30  NINDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGESQVVAGKKYCLVIEVKETII 89
           N ND  V+ + RFAV EH  +++      +LE+ R++  ++QVVAG  + L +EV +   
Sbjct: 19  NANDLQVESLARFAVDEHNKNEN-----LTLEYKRLLGAKTQVVAGTMHHLTVEVAD--- 78

Query: 90  IPSMPSSIKYFKAIVLDKPWEKSWELLSF 119
                 + K ++A VL+K WE   +L SF
Sbjct: 79  ----GETNKVYEAKVLEKAWENLKQLESF 95

BLAST of MS011276 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 45.1 bits (105), Expect = 4.8e-05
Identity = 35/109 (32.11%), Postives = 51/109 (46.79%), Query Frame = 0

Query: 12  HNDALLNVQKPGGWEPIKN--INDPYVQEMGRFAVMEHTHSKHGIICGASLEFIRVVSGE 71
           +ND ++ + K GG+   KN       + ++  FAV EH   ++ +     LE  RV+   
Sbjct: 36  YNDNVVKM-KLGGFSDSKNDWNGGKEIDDIALFAVQEHNRRENAV-----LELARVLKAT 95

Query: 72  SQVVAGKKYCLVIEVKETIIIPSMPSSIKYFKAIVLDKPWEKSWELLSF 119
            QVVAGK Y L +EV E           K ++A V  KPW    +L  F
Sbjct: 96  EQVVAGKLYRLTLEVIEA-------GEKKIYEAKVWVKPWMNFKQLQEF 131