MS011236 (gene) Bitter gourd (TR) v1

Overview


Name	MS011236
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	lipid droplet-associated hydrolase
Location	scaffold239: 314903 .. 318510 (-)
RNA-Seq Expression	MS011236
Synteny	MS011236

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTGAAGAAATTCTCAAGGTGTGCGTTCGTTCATATGGGTCGAGAAGTTGTTCATTCTTTCAGTAGAAGACATGCGGAGTTCCGTTTCTGCAATGTGTCAGGGTAATTCAGTCTCGTTTATGTTGATTGAAATTTCTCTTGAAACTGCACTTTTTGTAAATTGAATCGCCTCCATTTCTGGGGTTTTGATAGTTTCACCAATGAATTGCTGGAGATTCATTCGGATGATCCATCATTGCACGTATTGTTCATACCTGGAAATCCAGGTGCTTATGTATGCTTCTTTGGATGTGGGTGTTTCGATGTTTCTGTTGATTTTTCGTATAGTTTCTGAAGGTGGATTGTTTTGGGTTTCGTTAGGAATTATTTCATTTTATAAAGATTTTCTGGAATCGCTATATCAGTTGCTTGGAGGCCGAGTGTCTATAACAGGCAAGACATGAGTTGTTGACATTTATTCATATACCTATAATTTTTTTTTAAGGAAACATTAAATCTTTTCTGCTGTTATATTTGGGCTGACGCTTTTTCTTGTTCCATTTTTCCCTTTTTGGCTCTCCTTTTGTTTACTGTCAGCTATTGGGCATGTATCTCAAACGAAGAAGGTACTTGGATACGGGTCATATGAAGTTTATCTCTGTTATTTACATTTTAGCTTTCTTCTTTGTCTTCAGAATTACAAACGTCTGGACACAGAGGCTTAACTAGGGAGAGAGAGTGAAGTTTTTCAATTCAGAAATCTTTCTTAGTTGCTAATTGTCATAAAGTAATGAGGGAATAACATTTGGGAAAGATAGCAACATAATTTATCAAAAAAGTCGGATAAATTCAAATTTCAAGATAATTTCATTACTTTTTTGCATTATCTTACATATGGTAAGTTGCTTCTCTCTATCTGTATCATCTTTCCTTTGCTGAACATCTAAGACTATGTTATATAGATGATGATCAGAAATGTTACTTATTTGGATAGGAACAATGATAATTATCATTTACTTTTCAATGAAGGATGTTGATAGGTCTAAATGAGAATAGTTTAAGCAATGCATTGCCATGCTTTCCTATCAATATGCATGTTGATAATGTTTTTCCTCTAGAAATGATGGCTTTGTGCGCTGCATTTTTATATATGAACTATAAGTTGCTCTCTTTAATTATTGACTATGGTCAGACATGCTTAATTTTAGTGATTTTAAAGCAAATGGGTTGATGTTGCTCGGATGCATATTATTCAGTTTGTCCATATAAAATTCCCCAACGATTTTGTGTAATGGATATTGATAATTCTTCTTTTAGGACTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGGTAATTTTATGTTTTCTTTGATGATTTGATTGAGTTGTTAATGGGTTAAGGTGACAAGTGGTCCATGAAAAATTTGAGGCATTCCATGATACCATTGCAGAACTGTGAAACGAAGATCCTTTTACTCAAGATTATATTTCAAATCATTATTATCAGTTTCCTGCAGGTCCCTGCAATTTCATATTTGAATTGTTGATACCAGAATTCTGTATGGGCCTGAACTATATTCACCTTTATTTTGCAGATTGAATTTGTCAGACAAGAATTACCGAACAATGACATTCCACTACTACTGGTAACAATAGTTTTATGATTATATTGATGCTTTACTTTCACAATAAAAAAAAAAAAAGGAGTCGCAGGATGCAAAATTTTCAAAATTTCTTAGTTTGATAGTAAGCTCTACAATCTGTATAAATTTTCATTCTAGTTCAACCCAAAAACATTAGTTACTTGTGGTGCTTGTATGATTATTGTTAAATTGTTAACTAAAACAAGACAATGCACTTTATTGTATGTACGTGTACATTAAATGAATTATGAATATAATGAATTGGAAAAAAATATTATACGGGCCTTTAAATCGGAATTAAGCTGTTACATTTTTGCCAGTTTATTTGCTATGTTCGTTTTGGTTGCTGACTCGTCATTTCTATTTAAAGGTCGGACATTCTATTGGATCATATATCTCCATTGAATTGTTCCGTAGGTTACAAGATCAGGTATTGCCATAAACTCTTTATGCCTTCTATGTGTTTCCAACTTTCAAGAGATGGCTCAATGGCAATTTTTCTTGATCCTATAGGTGGTGTATTGCATTGGATTGCATCCATTTTTGATGATTAACAAACAATCTCGTCAGCAATTGCTTATTGGAAAATTTGCAGGGTATGGCCGTTAGTTTTTTTCTGATAACATGAATCACGCCTTCCTGCATTTACATTTTAGATTTTTTAGCAGCAATTTTATTGCATATCATGATTTTCAAAAATTCCCATTACATTTATATATTGATGAAAGGAGTTTGGCGTCCGGTGCCATAACAGCCATCTGCAGTATATAAGAGCATATAAGTGTGGTCATAAAACAGCCATTTTATAAGTTCGTGTTAGACTTTTGGTATTGCAAGTCGAGCCTTTCTGAGCACCACTAAGTTCATTTGGATTATCTGTCGTATTGTTTTCCTGCACCAACCAAAGGTTTAATGGCACTCTGTAAGGACTGGCTAGACAGTTTTTGTGTTGCAGATCCCCACTGCTATCGACTCTCTTTTCTGCTCTTACCGCATTGGTGGGTGCATTACCGAGACGAGCTCTAAGTTTTGTTGTGAGAAAAACTATTGCAAAGTCATGGTCAAATACTGCAACCGAGGCTGCTTGCTCTCACTTGCTAAAGGTGAAATCTAATTTATGGTTTCTAGTATGAGCTTTTGTTAGTATCCTCTTGTAACTGATGTATGTTCTGAGATTTGGAATATAATCTTTGTTTTCTACAGTACCATACCATGCGAAATGTGCTATTTATGGCGATGACAGAATTTAAAGAGGTACAGACTATGAACGAACAGTGAACACTTTAGTTTGGTGAACATGTCTACTATATATTGGATACCATGATGCTTCGACACTTTTTGCTTTTTATTGGAAGAAAAAGAAGTATAAGAAAGCATAATAAATTTCTGAGAGTTAAAAACTCCCGACTTACTTTCTAAGCACGTAAATGTACAAAGCCTAATATCAAGATCTAATCAAGTTTTCACATCTGATTGTTTATGCGCATTGTTTAGCTTTCAGAAGCACCAGATTGGGCATTTATGAAGAAAGCGAGTCAGAAATTATCATTTTTGTTTTGCATTGATGACCACTGGGCTCCAATGCATATGTATGAGGAGGTGAGATTTATACCATTTTTCCATCTTATGGATTCTATTCTGCTGCATCTATGTAGTTTCTCCAAATACTATTGCTGAAGAGATTCCATTTGTATCGTTATAATCGGTTCGAAATTAGTTTCGAAGTAGCGCGGTTGAAATACTAAAAAGGGGAGGGTCAGAAGGGCCAAAAACTATGACTGGAATGGTGATTTCAAGTTTTACTTTCTTTGCGTTTCTTATTTTCCCCACAGATTTCCAAGCAGGTCCCTGAGATTGATCTCTCAGTTGAAAGGGAGGGCCATACACATGCATTTTGCTGCTCAGAGGCTGCCTCACTGTATATTGCTCAACATGTAGCAAATTTGATCAAGAATCATCTCCCAGAT

mRNA sequence

AGATTGAAGAAATTCTCAAGGTGTGCGTTCGTTCATATGGGTCGAGAAGTTGTTCATTCTTTCAGTAGAAGACATGCGGAGTTCCGTTTCTGCAATGTGTCAGGTTTCACCAATGAATTGCTGGAGATTCATTCGGATGATCCATCATTGCACGTATTGTTCATACCTGGAAATCCAGGAATTATTTCATTTTATAAAGATTTTCTGGAATCGCTATATCAGTTGCTTGGAGGCCGAGACTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGATTGAATTTGTCAGACAAGAATTACCGAACAATGACATTCCACTACTACTGGTCGGACATTCTATTGGATCATATATCTCCATTGAATTGTTCCGTAGGTTACAAGATCAGGTGGTGTATTGCATTGGATTGCATCCATTTTTGATGATTAACAAACAATCTCGTCAGCAATTGCTTATTGGAAAATTTGCAGGATCCCCACTGCTATCGACTCTCTTTTCTGCTCTTACCGCATTGGTGGGTGCATTACCGAGACGAGCTCTAAGTTTTGTTGTGAGAAAAACTATTGCAAAGTCATGGTCAAATACTGCAACCGAGGCTGCTTGCTCTCACTTGCTAAAGTACCATACCATGCGAAATGTGCTATTTATGGCGATGACAGAATTTAAAGAGCTTTCAGAAGCACCAGATTGGGCATTTATGAAGAAAGCGAGTCAGAAATTATCATTTTTGTTTTGCATTGATGACCACTGGGCTCCAATGCATATGTATGAGGAGATTTCCAAGCAGGTCCCTGAGATTGATCTCTCAGTTGAAAGGGAGGGCCATACACATGCATTTTGCTGCTCAGAGGCTGCCTCACTGTATATTGCTCAACATGTAGCAAATTTGATCAAGAATCATCTCCCAGAT

Coding sequence (CDS)

AGATTGAAGAAATTCTCAAGGTGTGCGTTCGTTCATATGGGTCGAGAAGTTGTTCATTCTTTCAGTAGAAGACATGCGGAGTTCCGTTTCTGCAATGTGTCAGGTTTCACCAATGAATTGCTGGAGATTCATTCGGATGATCCATCATTGCACGTATTGTTCATACCTGGAAATCCAGGAATTATTTCATTTTATAAAGATTTTCTGGAATCGCTATATCAGTTGCTTGGAGGCCGAGACTGGGAAGGTGGAAGGTTATTTTCATTACAAGAACAAATTGATCATAAGATTGAATTTGTCAGACAAGAATTACCGAACAATGACATTCCACTACTACTGGTCGGACATTCTATTGGATCATATATCTCCATTGAATTGTTCCGTAGGTTACAAGATCAGGTGGTGTATTGCATTGGATTGCATCCATTTTTGATGATTAACAAACAATCTCGTCAGCAATTGCTTATTGGAAAATTTGCAGGATCCCCACTGCTATCGACTCTCTTTTCTGCTCTTACCGCATTGGTGGGTGCATTACCGAGACGAGCTCTAAGTTTTGTTGTGAGAAAAACTATTGCAAAGTCATGGTCAAATACTGCAACCGAGGCTGCTTGCTCTCACTTGCTAAAGTACCATACCATGCGAAATGTGCTATTTATGGCGATGACAGAATTTAAAGAGCTTTCAGAAGCACCAGATTGGGCATTTATGAAGAAAGCGAGTCAGAAATTATCATTTTTGTTTTGCATTGATGACCACTGGGCTCCAATGCATATGTATGAGGAGATTTCCAAGCAGGTCCCTGAGATTGATCTCTCAGTTGAAAGGGAGGGCCATACACATGCATTTTGCTGCTCAGAGGCTGCCTCACTGTATATTGCTCAACATGTAGCAAATTTGATCAAGAATCATCTCCCAGAT

Protein sequence

RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGRDWEGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLSTLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKNHLPD

Homology

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BLAST of MS011236 vs. NCBI nr
Match: XP_022132976.1 (lipid droplet-associated hydrolase [Momordica charantia])

HSP 1 Score: 616.3 bits (1588), Expect = 1.4e-172
Identity = 306/321 (95.33%), Postives = 307/321 (95.64%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           +LKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG
Sbjct: 36  KLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 95

Query: 61  IISFYKDFLESLYQLLGGR--------------DWEGGRLFSLQEQIDHKIEFVRQELPN 120
           IISFYKDFLESLYQLLGGR              DWEGGRLFSLQEQIDHKIEFVRQELPN
Sbjct: 96  IISFYKDFLESLYQLLGGRVSITAIGHVSQTKKDWEGGRLFSLQEQIDHKIEFVRQELPN 155

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
           NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS
Sbjct: 156 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 215

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK
Sbjct: 216 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 275

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS
Sbjct: 276 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 335

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EAASLYIAQHVANLIKNHLPD
Sbjct: 336 EAASLYIAQHVANLIKNHLPD 356

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BLAST of MS011236 vs. NCBI nr
Match: XP_023517065.1 (lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023517066.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023517067.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023517068.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527.3 bits (1357), Expect = 8.7e-146
Identity = 252/321 (78.50%), Postives = 284/321 (88.47%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLKKFSR AFVHMG+EV++ FSRR A+FR CNVSG+TNELLEIH+DDPSLHVLFIPGNPG
Sbjct: 30  RLKKFSRSAFVHMGQEVLNPFSRRRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPG 89

Query: 61  IISFYKDFLESLYQLLGGR--------------DWEGGRLFSLQEQIDHKIEFVRQELPN 120
           I+SFYKDF+ESLYQLLGGR              DWEGGRLFSLQEQIDHKIEFVRQEL N
Sbjct: 90  IVSFYKDFVESLYQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQN 149

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPLLLVGHSIGSYISIEL+R+ QDQVVYCIGLHPF+M+N+QSRQQ LIGK AGS LLS
Sbjct: 150 KDIPLLLVGHSIGSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLS 209

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFS+  AL+G LP R  SFVV KTI KSWS TA+EAAC++LLKYH MRN+LFMA+TEF+
Sbjct: 210 TLFSSFVALLGVLPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFE 269

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           +LSE PDWAFMKK+S+KLSFLFC+DDHWAPMH++EEISKQ+PE+D+SVEREGHTHAFCCS
Sbjct: 270 KLSETPDWAFMKKSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREGHTHAFCCS 329

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EA SLYIAQHVA+LIKNH+ D
Sbjct: 330 EAGSLYIAQHVASLIKNHISD 350

[Close]

BLAST of MS011236 vs. NCBI nr
Match: XP_004143334.1 (lipid droplet-associated hydrolase isoform X1 [Cucumis sativus] >KGN48293.1 hypothetical protein Csa_004459 [Cucumis sativus])

HSP 1 Score: 519.2 bits (1336), Expect = 2.4e-143
Identity = 249/321 (77.57%), Postives = 278/321 (86.60%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLK FSRC+F+HMG +V  SFS+R  EFR CNVSGFTNELLEIHSDDPSLHVLFIPGNPG
Sbjct: 22  RLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 81

Query: 61  IISFYKDFLESLYQLLGG--------------RDWEGGRLFSLQEQIDHKIEFVRQELPN 120
           IISFYKDFLESLYQLLGG              +DWEGGRLFSLQEQIDHK+EFVRQEL N
Sbjct: 82  IISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQN 141

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPLLLVGHS+GSYISIELFRR QD+ VYCIGLHPF+M+N++SRQQ  I K + SPLLS
Sbjct: 142 KDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLS 201

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFS+  AL+G LP +A SFVV+KTI KSWS TA+EAACSHLLKYH+MRNVL+MAMTEF 
Sbjct: 202 TLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFD 261

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           + SE PDWAFMKK SQKLSFLFC+DDHWAPMH+YEEISKQVPEIDLSVEREG++HAFCCS
Sbjct: 262 KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCS 321

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EAAS+YIAQ+VA+L+K HL D
Sbjct: 322 EAASMYIAQYVASLVKKHLSD 342

[Close]

BLAST of MS011236 vs. NCBI nr
Match: KAA0025227.1 (lipid droplet-associated hydrolase [Cucumis melo var. makuwa] >TYK07439.1 lipid droplet-associated hydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 517.7 bits (1332), Expect = 6.9e-143
Identity = 248/321 (77.26%), Postives = 277/321 (86.29%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLK FSRC+F+HMG +V+ SFS+R  EFR CNVSGFTNELLEIHSDDPSLHVLFIPGNPG
Sbjct: 22  RLKNFSRCSFLHMGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 81

Query: 61  IISFYKDFLESLYQLLGG--------------RDWEGGRLFSLQEQIDHKIEFVRQELPN 120
           IISFYKDF+ESLYQLLGG              +DWEGGRLFSLQEQIDHK+EFVRQEL N
Sbjct: 82  IISFYKDFVESLYQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQN 141

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPL+LVGHS+GSYISIELFRR QD+ VYCIGLHPF+M+NK+SRQQ  I K A SPLLS
Sbjct: 142 KDIPLILVGHSVGSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLS 201

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           T FS+  AL+G LP +A SFVVRKTI KSWS TA EAACSHLLKYH+MRNVL+MAMTEF+
Sbjct: 202 TFFSSFVALLGILPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFE 261

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           + SE PDWAFMKK SQKLSFLFC+DDHWAPMH+YEEI KQVPEIDLSVEREG++HAFCCS
Sbjct: 262 KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVEREGYSHAFCCS 321

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EAAS+YIAQ+VA+L+K HL D
Sbjct: 322 EAASIYIAQYVASLVKKHLSD 342

[Close]

BLAST of MS011236 vs. NCBI nr
Match: XP_022972584.1 (lipid droplet-associated hydrolase isoform X1 [Cucurbita maxima] >XP_022972585.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita maxima] >XP_022972586.1 lipid droplet-associated hydrolase isoform X1 [Cucurbita maxima])

HSP 1 Score: 515.4 bits (1326), Expect = 3.4e-142
Identity = 247/321 (76.95%), Postives = 281/321 (87.54%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLKKFSR AFVHMG+EV++ FSR  A+FR CNVSG+TNELLEIH+DDPSLHVLFIPGNPG
Sbjct: 31  RLKKFSRSAFVHMGQEVLNPFSRWRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPG 90

Query: 61  IISFYKDFLESLYQLLGGR--------------DWEGGRLFSLQEQIDHKIEFVRQELPN 120
           I+SFYK F+ESLYQLLGGR              DWEGGRLFSLQEQIDHKIEFVRQEL +
Sbjct: 91  IVSFYKYFVESLYQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQS 150

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPLLLVGHSIGSYISIEL+R+ QD+VVYCIGLHPF+M+N+QSRQQ LIGK AGS LLS
Sbjct: 151 KDIPLLLVGHSIGSYISIELYRKFQDRVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLS 210

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFS+  AL+G LP R  SFVV KTI KSWS TA+EAAC++LLKYH MRN+LFMA+TEF+
Sbjct: 211 TLFSSFVALLGVLPSRVSSFVVGKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFE 270

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           +LSE PDW FMKK+S+KLSFLFC+DDHWAPMH++EEISKQ+PE+D+SVEREGHTHAFCCS
Sbjct: 271 KLSETPDWGFMKKSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREGHTHAFCCS 330

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EA SLYIAQHVA+LIKNH+ D
Sbjct: 331 EAGSLYIAQHVASLIKNHISD 351

[Close]

BLAST of MS011236 vs. ExPASy Swiss-Prot
Match: Q5HZX7 (Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-21
Identity = 85/290 (29.31%), Postives = 137/290 (47.24%), Query Frame = 0


Query: 51  HVLF-IPGNPGIISFYKDFLESLYQLLGGR--DW-------------------------- 110
           H++F IPGNPG+  FY  F ++LY L+ G    W                          
Sbjct: 45  HLIFVIPGNPGLSPFYVPFAKALYSLVKGHFPVWIISHAGFCLTPKDKKILTAPQEPNAQ 104

Query: 111 EGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLH 170
           E   ++ L  QI+HKI F+R  +P  D+ L+ +GHS+GSYI++ +  R  +  V    +H
Sbjct: 105 EIEDIYGLNGQIEHKIAFLRAHVP-KDVKLIFIGHSVGSYIALHVMNRAPELPV----VH 164

Query: 171 PFLMINKQSR-QQLLIGKFAGSPLLS----TLFSALTALVGALPRRALSFVVRKTIAKSW 230
            FL+     R  +   GKFA +P L      L++A   +    P    SF+V+K + K  
Sbjct: 165 TFLLFPTIERMSESPNGKFA-TPFLCRFRYMLYAASYLIFKPCPEMIKSFIVQKVLEK-- 224

Query: 231 SNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFLFCIDDHWAP 290
            +  +E   + LL+   + N  ++   E   + +  D   +K+   KL+F +   D W P
Sbjct: 225 MSFKSELRLTDLLQPFCLANAAYLGGQEMIHVVKRDD-GIIKELLPKLTFYYGKTDGWCP 284

Query: 291 MHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKNHLP 307
           ++ YE++ +  PE +L +  +G  HAF      S  +A  VA+ I N LP
Sbjct: 285 VNYYEDMKRDFPEANLHLCEKGIPHAFVL--GFSQEMAAMVADWINNRLP 323

[Close]

BLAST of MS011236 vs. ExPASy Swiss-Prot
Match: Q5F477 (Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 7.7e-20
Identity = 89/323 (27.55%), Postives = 153/323 (47.37%), Query Frame = 0


Query: 25  HAEFRFCNVSGFTNELL------EIHSDD----PSLHVLFIPGNPGIISFYKDFLESLY- 84
           H EF +C   G    +L      ++  D+    P L  + IPGNPG+  +Y+ F+++LY 
Sbjct: 12  HEEFIYC--CGAATHVLKCGPWKDLSKDESKNLPRLLFMIIPGNPGLAGYYRTFIKALYC 71

Query: 85  -----------------------QLLGGRD-WEGGRLFSLQEQIDHKIEFVRQELPNNDI 144
                                  +++   D  E   +F L  Q++HK+ F+++ + + DI
Sbjct: 72  GLNQQYPVWVVSHAGHCKPPSGMEMIEDTDIKELEDVFGLNGQVEHKLNFLKKNV-SKDI 131

Query: 145 PLLLVGHSIGSYISIELFRRLQD-QVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLSTL 204
            L+L+ HSIG YI++E+ +R  + QV+  + L P +    QS Q  L+     +PLL  L
Sbjct: 132 KLVLIAHSIGCYITLEMMKRASELQVLRSVLLFPTIERMAQSPQGKLM-----TPLLCKL 191

Query: 205 FSAL---TALVGALPRRALSFVVRKTIAKSWSNTATEAACS---HLLKYHTMRNVLFMAM 264
             AL     L+  LP    + +VR   A     T  E++ +   +L     + N+L+MA 
Sbjct: 192 RYALYMPVYLLSFLPEGVKASLVR--FALRGMKTCDESSITTSVNLFSVDCIANILYMAS 251

Query: 265 TEFKELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHA 306
            E  ++ E  D   +K+  +KL F +   D W P + Y+EI    P+ D+ +  +G  HA
Sbjct: 252 QEMMKVVER-DSTTIKQNLKKLIFYYGTGDSWCPQNYYDEIKMDFPDGDIRLCEKGLRHA 311

[Close]

BLAST of MS011236 vs. ExPASy Swiss-Prot
Match: Q8BVA5 (Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.4e-18
Identity = 82/314 (26.11%), Postives = 138/314 (43.95%), Query Frame = 0


Query: 27  EFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGGR--DW--- 86
           E +      +TN   +     P   +  IPGNPG  +FY  F ++LY L+  R   W   
Sbjct: 22  ETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTLMKSRFPVWIIS 81

Query: 87  ------------------------EGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHS 146
                                   +   ++ L  QI+HKI F+R  +P  D+ L+L+GHS
Sbjct: 82  HAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVP-KDVKLILIGHS 141

Query: 147 IGSYISIELFRRLQDQVVYCIGLHPFLMINKQSR-QQLLIGKFAGSPLLS----TLFSAL 206
           +G+Y+++ + +R+ +  V     H FL+     R  +   GKFA +P L      L++  
Sbjct: 142 VGTYMTLHVMKRVLELPV----AHAFLLFPTIERMSESPNGKFA-TPFLCQFRYLLYATS 201

Query: 207 TALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAP 266
             L    P    SF+++K + +   N   E   + +L+   + N  ++   E  ++ +  
Sbjct: 202 YLLFKPCPEVIKSFIIQKLMGQ--MNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRD 261

Query: 267 DWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLY 307
           D   +K+   KL F +   D W P+  YE++ K  PE ++ +  +G  HAF      S  
Sbjct: 262 D-DIIKEFLPKLKFYYGKTDGWCPVKYYEDMKKDFPEGNIYLCEKGIPHAFVLD--FSQE 321

[Close]

BLAST of MS011236 vs. ExPASy Swiss-Prot
Match: Q9H6V9 (Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.2e-18
Identity = 77/292 (26.37%), Postives = 128/292 (43.84%), Query Frame = 0


Query: 48  PSLHVLFIPGNPGIISFYKDFLESLYQLLGGR--DW------------------------ 107
           P L +  IPGNPG  +FY  F ++LY L   R   W                        
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRRFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 108 --EGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELFRRLQDQVVYCIG 167
             E   ++ L  QI+HK+ F+R  +P  D+ L+L+GHSIGSY ++++ +R+ +  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSIGSYFTLQMLKRVPELPV---- 162

Query: 168 LHPFLMINKQSRQQLLIGKFAGSPLLS----TLFSALTALVGALPRRALSFVVRKTIAKS 227
           +  FL+     R          +PLL      L+     L+   P    S ++R+ +   
Sbjct: 163 IRAFLLFPTIERMSESPNGRIATPLLCWFRYVLYVTGYLLLKPCPETIKSLLIRRGL--Q 222

Query: 228 WSNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFLFCIDDHWA 287
             N   E +  ++L+   + N  ++   E  E+ +  D   +K+   KL+F +   D W 
Sbjct: 223 VMNLENEFSPLNILEPFCLANAAYLGGQEMMEVVKRDD-ETIKEHLCKLTFYYGTIDPWC 282

Query: 288 PMHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKNHLPD 308
           P   YE+I K  PE D+ +  +   HAF        +  Q +A++I + L D
Sbjct: 283 PKEYYEDIKKDFPEGDIRLCEKNIPHAFI------THFNQEMADMIADSLKD 320

[Close]

BLAST of MS011236 vs. ExPASy Swiss-Prot
Match: Q5R7E8 (Lipid droplet-associated hydrolase OS=Pongo abelii OX=9601 GN=LDAH PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 9.4e-18
Identity = 75/292 (25.68%), Postives = 130/292 (44.52%), Query Frame = 0


Query: 48  PSLHVLFIPGNPGIISFYKDFLESLYQL----------------LGGRD----------- 107
           P L +  IPGNPG  +FY  F ++LY L                L  +D           
Sbjct: 43  PKLLIFIIPGNPGFSAFYVPFAKALYSLTNRCFPVWTISHAGHALAPKDKKILTTSEDSN 102

Query: 108 -WEGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELFRRLQDQVVYCIG 167
             E   ++ L  QI+HK+ F+R  +P  D+ L+L+GHS+GSY ++++ +R+ +  V    
Sbjct: 103 AQEIKDIYGLNGQIEHKLAFLRTHVP-KDMKLVLIGHSVGSYFTLQMLKRVPELPV---- 162

Query: 168 LHPFLMINKQSRQQLLIGKFAGSPLLS----TLFSALTALVGALPRRALSFVVRKTIAKS 227
           +  FL+     R          +PLL      L+     L+   P +  S ++R+ +   
Sbjct: 163 IRAFLLFPTIERMSESPNGRIATPLLCWSRYVLYVTGYLLLKPCPEKIKSLLIRRGL--Q 222

Query: 228 WSNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFLFCIDDHWA 287
             N   E +  ++L+   + N   +   E  E+ +  D   +++   KL+F +   D W 
Sbjct: 223 VMNLENEFSPLNILEPFCLANAAHLGGQEMMEVVKRDD-ETIREHLCKLTFYYGTIDPWC 282

Query: 288 PMHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKNHLPD 308
           P   YE+I K  PE D+ +  +   HAF       ++  Q +A++I + L D
Sbjct: 283 PKEYYEDIKKDFPEGDIRLCEKNIPHAFI------IHFNQEMADMIADSLKD 320

[Close]

BLAST of MS011236 vs. ExPASy TrEMBL
Match: A0A6J1BTR5 (lipid droplet-associated hydrolase OS=Momordica charantia OX=3673 GN=LOC111005683 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 6.9e-173
Identity = 306/321 (95.33%), Postives = 307/321 (95.64%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           +LKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG
Sbjct: 36  KLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 95

Query: 61  IISFYKDFLESLYQLLGGR--------------DWEGGRLFSLQEQIDHKIEFVRQELPN 120
           IISFYKDFLESLYQLLGGR              DWEGGRLFSLQEQIDHKIEFVRQELPN
Sbjct: 96  IISFYKDFLESLYQLLGGRVSITAIGHVSQTKKDWEGGRLFSLQEQIDHKIEFVRQELPN 155

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
           NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS
Sbjct: 156 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 215

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK
Sbjct: 216 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 275

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS
Sbjct: 276 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 335

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EAASLYIAQHVANLIKNHLPD
Sbjct: 336 EAASLYIAQHVANLIKNHLPD 356

[Close]

BLAST of MS011236 vs. ExPASy TrEMBL
Match: A0A0A0KFF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 249/321 (77.57%), Postives = 278/321 (86.60%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLK FSRC+F+HMG +V  SFS+R  EFR CNVSGFTNELLEIHSDDPSLHVLFIPGNPG
Sbjct: 22  RLKNFSRCSFLHMGHQVPQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 81

Query: 61  IISFYKDFLESLYQLLGG--------------RDWEGGRLFSLQEQIDHKIEFVRQELPN 120
           IISFYKDFLESLYQLLGG              +DWEGGRLFSLQEQIDHK+EFVRQEL N
Sbjct: 82  IISFYKDFLESLYQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQN 141

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPLLLVGHS+GSYISIELFRR QD+ VYCIGLHPF+M+N++SRQQ  I K + SPLLS
Sbjct: 142 KDIPLLLVGHSVGSYISIELFRRFQDRAVYCIGLHPFMMVNRESRQQFFIEKLSRSPLLS 201

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFS+  AL+G LP +A SFVV+KTI KSWS TA+EAACSHLLKYH+MRNVL+MAMTEF 
Sbjct: 202 TLFSSFAALLGTLPIQASSFVVKKTIGKSWSRTASEAACSHLLKYHSMRNVLYMAMTEFD 261

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           + SE PDWAFMKK SQKLSFLFC+DDHWAPMH+YEEISKQVPEIDLSVEREG++HAFCCS
Sbjct: 262 KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEISKQVPEIDLSVEREGYSHAFCCS 321

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EAAS+YIAQ+VA+L+K HL D
Sbjct: 322 EAASMYIAQYVASLVKKHLSD 342

[Close]

BLAST of MS011236 vs. ExPASy TrEMBL
Match: A0A5A7SKD4 (Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001690 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.3e-143
Identity = 248/321 (77.26%), Postives = 277/321 (86.29%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLK FSRC+F+HMG +V+ SFS+R  EFR CNVSGFTNELLEIHSDDPSLHVLFIPGNPG
Sbjct: 22  RLKNFSRCSFLHMGHQVLQSFSKRRVEFRLCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 81

Query: 61  IISFYKDFLESLYQLLGG--------------RDWEGGRLFSLQEQIDHKIEFVRQELPN 120
           IISFYKDF+ESLYQLLGG              +DWEGGRLFSLQEQIDHK+EFVRQEL N
Sbjct: 82  IISFYKDFVESLYQLLGGHVSITAIGHICQTKKDWEGGRLFSLQEQIDHKVEFVRQELQN 141

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPL+LVGHS+GSYISIELFRR QD+ VYCIGLHPF+M+NK+SRQQ  I K A SPLLS
Sbjct: 142 KDIPLILVGHSVGSYISIELFRRFQDRAVYCIGLHPFMMVNKESRQQFFIEKLARSPLLS 201

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           T FS+  AL+G LP +A SFVVRKTI KSWS TA EAACSHLLKYH+MRNVL+MAMTEF+
Sbjct: 202 TFFSSFVALLGILPIQASSFVVRKTIGKSWSRTACEAACSHLLKYHSMRNVLYMAMTEFE 261

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           + SE PDWAFMKK SQKLSFLFC+DDHWAPMH+YEEI KQVPEIDLSVEREG++HAFCCS
Sbjct: 262 KFSETPDWAFMKKVSQKLSFLFCMDDHWAPMHVYEEIFKQVPEIDLSVEREGYSHAFCCS 321

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EAAS+YIAQ+VA+L+K HL D
Sbjct: 322 EAASIYIAQYVASLVKKHLSD 342

[Close]

BLAST of MS011236 vs. ExPASy TrEMBL
Match: A0A6J1I921 (lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471128 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.7e-142
Identity = 247/321 (76.95%), Postives = 281/321 (87.54%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLKKFSR AFVHMG+EV++ FSR  A+FR CNVSG+TNELLEIH+DDPSLHVLFIPGNPG
Sbjct: 31  RLKKFSRSAFVHMGQEVLNPFSRWRAQFRLCNVSGYTNELLEIHADDPSLHVLFIPGNPG 90

Query: 61  IISFYKDFLESLYQLLGGR--------------DWEGGRLFSLQEQIDHKIEFVRQELPN 120
           I+SFYK F+ESLYQLLGGR              DWEGGRLFSLQEQIDHKIEFVRQEL +
Sbjct: 91  IVSFYKYFVESLYQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQS 150

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPLLLVGHSIGSYISIEL+R+ QD+VVYCIGLHPF+M+N+QSRQQ LIGK AGS LLS
Sbjct: 151 KDIPLLLVGHSIGSYISIELYRKFQDRVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLS 210

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TLFS+  AL+G LP R  SFVV KTI KSWS TA+EAAC++LLKYH MRN+LFMA+TEF+
Sbjct: 211 TLFSSFVALLGVLPSRVSSFVVGKTIGKSWSRTASEAACTYLLKYHVMRNILFMALTEFE 270

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           +LSE PDW FMKK+S+KLSFLFC+DDHWAPMH++EEISKQ+PE+D+SVEREGHTHAFCCS
Sbjct: 271 KLSETPDWGFMKKSSRKLSFLFCMDDHWAPMHVFEEISKQIPEMDVSVEREGHTHAFCCS 330

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EA SLYIAQHVA+LIKNH+ D
Sbjct: 331 EAGSLYIAQHVASLIKNHISD 351

[Close]

BLAST of MS011236 vs. ExPASy TrEMBL
Match: A0A6J1HG44 (lipid droplet-associated hydrolase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463638 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.7e-142
Identity = 247/321 (76.95%), Postives = 280/321 (87.23%), Query Frame = 0


Query: 1   RLKKFSRCAFVHMGREVVHSFSRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPG 60
           RLKKFSR AFVHMG+EV++ F RR A+FR CNVSG+TNEL EIH+DDPSLHVLFIPGNPG
Sbjct: 30  RLKKFSRSAFVHMGQEVLNPFIRRRAQFRLCNVSGYTNELPEIHADDPSLHVLFIPGNPG 89

Query: 61  IISFYKDFLESLYQLLGGR--------------DWEGGRLFSLQEQIDHKIEFVRQELPN 120
           I+SFYKDF+ESLYQLLGGR              DWEGGRLFSLQEQIDHKIEFVRQEL N
Sbjct: 90  IVSFYKDFVESLYQLLGGRVSITAIGHLSQTTKDWEGGRLFSLQEQIDHKIEFVRQELQN 149

Query: 121 NDIPLLLVGHSIGSYISIELFRRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLS 180
            DIPLLLVGHSIGSYISIEL+R+ QDQVVYCIGLHPF+M+N+QSRQQ LIGK AGS LLS
Sbjct: 150 KDIPLLLVGHSIGSYISIELYRKFQDQVVYCIGLHPFMMVNRQSRQQFLIGKLAGSRLLS 209

Query: 181 TLFSALTALVGALPRRALSFVVRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFK 240
           TL S+  AL+G LP R  SFVV KTI KSWS TA+EAAC++LLKYH MRN+LFMA++EF+
Sbjct: 210 TLVSSFVALLGVLPSRVSSFVVSKTIGKSWSRTASEAACTYLLKYHVMRNILFMALSEFE 269

Query: 241 ELSEAPDWAFMKKASQKLSFLFCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCS 300
           +LSE PDWAFMKK+S+KLSFLFC+DDHWAPMH++EEI KQ+PE+D+SVEREGHTHAFCCS
Sbjct: 270 KLSETPDWAFMKKSSRKLSFLFCMDDHWAPMHVFEEIWKQIPEMDVSVEREGHTHAFCCS 329

Query: 301 EAASLYIAQHVANLIKNHLPD 308
           EA SLYIAQHVA+LIKNH+ D
Sbjct: 330 EAGSLYIAQHVASLIKNHISD 350

[Close]

BLAST of MS011236 vs. TAIR 10
Match: AT3G11620.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 368.2 bits (944), Expect = 6.3e-102
Identity = 172/296 (58.11%), Postives = 220/296 (74.32%), Query Frame = 0


Query: 22  SRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGG--- 81
           ++RH + R C VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG   
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 82  -----------RDWEGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELF 141
                      +DWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 142 RRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLSTLFSALTALVGALPRRALSFV 201
           ++  D+VVYCIGL+PFL +N+QS +Q LIGK A S +LS   S L A +  LP  A   +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 202 VRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFL 261
           V K+I  SWS+TA +A C+HL +YHTMRNVLFMA +EF+EL+  PDW FM++   KL+FL
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMRENQSKLAFL 250

Query: 262 FCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKN 304
           F IDDHW P+ ++EEISKQ P   LS+EREGHTH FCC+ A S ++AQHVA LIKN
Sbjct: 251 FGIDDHWGPLQLFEEISKQAPHTSLSIEREGHTHGFCCTVAGSAWVAQHVATLIKN 306

[Close]

BLAST of MS011236 vs. TAIR 10
Match: AT3G11620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 368.2 bits (944), Expect = 6.3e-102
Identity = 172/296 (58.11%), Postives = 220/296 (74.32%), Query Frame = 0


Query: 22  SRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGG--- 81
           ++RH + R C VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG   
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 82  -----------RDWEGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELF 141
                      +DWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 142 RRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLSTLFSALTALVGALPRRALSFV 201
           ++  D+VVYCIGL+PFL +N+QS +Q LIGK A S +LS   S L A +  LP  A   +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 202 VRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFKELSEAPDWAFMKKASQKLSFL 261
           V K+I  SWS+TA +A C+HL +YHTMRNVLFMA +EF+EL+  PDW FM++   KL+FL
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFRELAAEPDWDFMRENQSKLAFL 250

Query: 262 FCIDDHWAPMHMYEEISKQVPEIDLSVEREGHTHAFCCSEAASLYIAQHVANLIKN 304
           F IDDHW P+ ++EEISKQ P   LS+EREGHTH FCC+ A S ++AQHVA LIKN
Sbjct: 251 FGIDDHWGPLQLFEEISKQAPHTSLSIEREGHTHGFCCTVAGSAWVAQHVATLIKN 306

[Close]

BLAST of MS011236 vs. TAIR 10
Match: AT3G11620.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 125/221 (56.56%), Postives = 163/221 (73.76%), Query Frame = 0


Query: 22  SRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGG--- 81
           ++RH + R C VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG   
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 82  -----------RDWEGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELF 141
                      +DWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 142 RRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLSTLFSALTALVGALPRRALSFV 201
           ++  D+VVYCIGL+PFL +N+QS +Q LIGK A S +LS   S L A +  LP  A   +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 202 VRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFKEL 229
           V K+I  SWS+TA +A C+HL +YHTMRNVLFMA +EF+E+
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFREV 231

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BLAST of MS011236 vs. TAIR 10
Match: AT3G11620.4 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 125/221 (56.56%), Postives = 163/221 (73.76%), Query Frame = 0


Query: 22  SRRHAEFRFCNVSGFTNELLEIHSDDPSLHVLFIPGNPGIISFYKDFLESLYQLLGG--- 81
           ++RH + R C VSG   E++EI +++P+ HVLFIPGNPG++SFYKDFLESLY+ LGG   
Sbjct: 11  TKRHVKSRLCRVSGSMTEMMEIQAENPTFHVLFIPGNPGVVSFYKDFLESLYEFLGGNAS 70

Query: 82  -----------RDWEGGRLFSLQEQIDHKIEFVRQELPNNDIPLLLVGHSIGSYISIELF 141
                      +DWE GRLFS QEQIDHKI+F+RQEL +  +P++LVGHSIGSYIS+EL 
Sbjct: 71  VIAIGQISHTSKDWESGRLFSFQEQIDHKIDFIRQELESVKVPIILVGHSIGSYISLELL 130

Query: 142 RRLQDQVVYCIGLHPFLMINKQSRQQLLIGKFAGSPLLSTLFSALTALVGALPRRALSFV 201
           ++  D+VVYCIGL+PFL +N+QS +Q LIGK A S +LS   S L A +  LP  A   +
Sbjct: 131 KKFSDKVVYCIGLYPFLTLNQQSTKQSLIGKLAASSVLSATASFLIASLRLLPMSAARLL 190

Query: 202 VRKTIAKSWSNTATEAACSHLLKYHTMRNVLFMAMTEFKEL 229
           V K+I  SWS+TA +A C+HL +YHTMRNVLFMA +EF+E+
Sbjct: 191 VSKSIGASWSDTAVQATCTHLRQYHTMRNVLFMAKSEFREV 231

The following BLAST results are available for this feature:

BLAST of MS011236 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132976.1	1.4e-172	95.33	lipid droplet-associated hydrolase [Momordica charantia]	[more]
XP_023517065.1	8.7e-146	78.50	lipid droplet-associated hydrolase isoform X1 [Cucurbita pepo subsp. pepo] >XP_0...	[more]
XP_004143334.1	2.4e-143	77.57	lipid droplet-associated hydrolase isoform X1 [Cucumis sativus] >KGN48293.1 hypo...	[more]
KAA0025227.1	6.9e-143	77.26	lipid droplet-associated hydrolase [Cucumis melo var. makuwa] >TYK07439.1 lipid ...	[more]
XP_022972584.1	3.4e-142	76.95	lipid droplet-associated hydrolase isoform X1 [Cucurbita maxima] >XP_022972585.1...	[more]

BLAST of MS011236 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5HZX7	1.8e-21	29.31	Lipid droplet-associated hydrolase OS=Rattus norvegicus OX=10116 GN=Ldah PE=2 SV...	[more]
Q5F477	7.7e-20	27.55	Lipid droplet-associated hydrolase OS=Gallus gallus OX=9031 GN=LDAH PE=2 SV=2	[more]
Q8BVA5	1.4e-18	26.11	Lipid droplet-associated hydrolase OS=Mus musculus OX=10090 GN=Ldah PE=1 SV=1	[more]
Q9H6V9	3.2e-18	26.37	Lipid droplet-associated hydrolase OS=Homo sapiens OX=9606 GN=LDAH PE=1 SV=1	[more]
Q5R7E8	9.4e-18	25.68	Lipid droplet-associated hydrolase OS=Pongo abelii OX=9601 GN=LDAH PE=2 SV=1	[more]

BLAST of MS011236 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTR5	6.9e-173	95.33	lipid droplet-associated hydrolase OS=Momordica charantia OX=3673 GN=LOC11100568...	[more]
A0A0A0KFF6	1.1e-143	77.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454400 PE=3 SV=1	[more]
A0A5A7SKD4	3.3e-143	77.26	Lipid droplet-associated hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1I921	1.7e-142	76.95	lipid droplet-associated hydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1HG44	1.7e-142	76.95	lipid droplet-associated hydrolase isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of MS011236 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11620.2	6.3e-102	58.11	alpha/beta-Hydrolases superfamily protein	[more]
AT3G11620.1	6.3e-102	58.11	alpha/beta-Hydrolases superfamily protein	[more]
AT3G11620.3	1.1e-69	56.56	alpha/beta-Hydrolases superfamily protein	[more]
AT3G11620.4	1.1e-69	56.56	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019363	Lipid droplet-associated hydrolase	PFAM	PF10230	LIDHydrolase	coord: 84..284 e-value: 2.3E-48 score: 165.1 coord: 50..76 e-value: 6.7E-7 score: 29.1
IPR019363	Lipid droplet-associated hydrolase	PANTHER	PTHR13390	LIPASE	coord: 28..305
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 22..304 e-value: 6.8E-11 score: 44.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 46..298
None	No IPR available	PANTHER	PTHR13390:SF2	BNAA05G27450D PROTEIN	coord: 28..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011236.1	MS011236.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019915	lipid storage
cellular_component	GO:0005811	lipid droplet
molecular_function	GO:0016298	lipase activity