MS011228 (gene) Bitter gourd (TR) v1

Overview
NameMS011228
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPentatricopeptide repeat (PPR-like) superfamily protein
Locationscaffold239: 262786 .. 264366 (+)
RNA-Seq ExpressionMS011228
SyntenyMS011228
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCAAGACCCCCATTGACCTCAAGCAAGTCCATGCCCGTATCGTCCAGAAAGGACTCGAGCAAGATCATTTCCTCGTGACCCAATTCATTTCATCGTCCAATTCATTTGCCAACATCTTCTATTCCACATCCGTCTTTGATCGTGTTGTCTCACCCAGTACCTTTCTTTGGAACTCTCTTGTCCGTGGATACTGTAAGAAGTTGCATTTTGTTGATACGATTTCGCTATTTGTGCGTATGAAAAGGGAGGACGGCGTTCCTGATCGGTATACGTTCCCTTCGCTTCTCAAGGCGTGCGCGAGTGAGGGGAAGGTGATTGAAGGGATGGTCCTTCACGGATCCATCTTGAGATGTGGGGTTGATGGGGATTTATATGTGACGACGAGTTTAGTGAACGTGTATGGGAAGGGTGGGTTAGTCGACTGTGCTCGTAAGGTGTTTGATGGGATGTCGCAGAAGAATGTGGTTTCTTGGACGGCTATGATTGTTGGGTATTCGAATATTGGGAATTTGGTGGAGGCAAAGAAGCTTTTCGATTCGATGCCGGAGAGAAATGTGGCGTCGTGGAATGCACTCATAGGAGGGTGCATGAAAATGGGTGATCTAAAGAGCGCTGAGAAGGTATTCGATGAAATGCCAGAAAAGAATGTTGTATCTTTCACAACAATGATCGATGGGTATGCTAAAGCCGGCGACATGGTTTCTGCCAGGAAATTATTTCAACAAGCTCCTGAGAGAGATATCGTCGCATGGTCGGCTTTGATATCTGGGTATACACAAAATGGACATCCAAATGAGGCAGTCGAGACATTTCTCGAAATGGGTTTAAGGAATGTGAAACCTGATGAGTTTGTATTGGCAAGCTTGATGTCAGCTTGTTCCCAAGTGGGTAATTTGGATTTAGCTAAATGGGTTGATTCATATGCTACCAAGTTATTGATTGATCTTCGTGGAGCTCACGTTAGGGCAGCTCTTATAGACATGAACGCCAAGTGTGGAAACATGGAGAGAGCCATGAATCTGTTTGATGAAATGCCTAAAAGAGATCTAATTTCCTATTGTTCTATAATGCAAGGGTTGTCGATCCACGGGCGTGGCGATCAAGCTGTGTCACTCTTCGAGAGGATGCTGGATGAAGGTCTAACTCCCGATGAAGTAGCCTTCACAGTCATCTTGACAGCTTGTAGCCGTGCTGGGTTCGTGGATGAAGGTTGGCATTACTTTGAATTCATGAGGAGTAAATACTCCATGATTCCCTCTCCTGATCACTATGCATGTATTGTTGATCTTCTTAGTCGATCTGGGAGACTAAAAGCAGCTTACGAGCTTATAAAATCCGTGCCTGTTCAATCCCATGCTGGTGCATGGGGTGCACTGCTAGGGGCCTGCAAATTATATTGCAATTCTGAGCTTGCAGAAGTGGTCGCTTCTCGACTTATTGAGCTTGAGCCTCAAAATGCTGGCAACTATGTGCTGTTATCAAATATCTATGCTGCAGCAGAAAGGTGGTTGGATGTGTCTGTCGTAAGAAACCAAATGAATGAGAGAGGGGTTAGAAAAATACCTGGTTGCAGTTGGATT

mRNA sequence

TGCAAGACCCCCATTGACCTCAAGCAAGTCCATGCCCGTATCGTCCAGAAAGGACTCGAGCAAGATCATTTCCTCGTGACCCAATTCATTTCATCGTCCAATTCATTTGCCAACATCTTCTATTCCACATCCGTCTTTGATCGTGTTGTCTCACCCAGTACCTTTCTTTGGAACTCTCTTGTCCGTGGATACTGTAAGAAGTTGCATTTTGTTGATACGATTTCGCTATTTGTGCGTATGAAAAGGGAGGACGGCGTTCCTGATCGGTATACGTTCCCTTCGCTTCTCAAGGCGTGCGCGAGTGAGGGGAAGGTGATTGAAGGGATGGTCCTTCACGGATCCATCTTGAGATGTGGGGTTGATGGGGATTTATATGTGACGACGAGTTTAGTGAACGTGTATGGGAAGGGTGGGTTAGTCGACTGTGCTCGTAAGGTGTTTGATGGGATGTCGCAGAAGAATGTGAATGTTGTATCTTTCACAACAATGATCGATGGGTATGCTAAAGCCGGCGACATGGTTTCTGCCAGGAAATTATTTCAACAAGCTCCTGAGAGAGATATCGTCGCATGGTCGGCTTTGATATCTGGGTATACACAAAATGGACATCCAAATGAGGCAGTCGAGACATTTCTCGAAATGGGTTTAAGGAATGTGAAACCTGATGAGTTTGTATTGGCAAGCTTGATGTCAGCTTGTTCCCAAGTGGGTAATTTGGATTTAGCTAAATGGGTTGATTCATATGCTACCAAGTTATTGATTGATCTTCGTGGAGCTCACGTTAGGGCAGCTCTTATAGACATGAACGCCAAGTGTGGAAACATGGAGAGAGCCATGAATCTGTTTGATGAAATGCCTAAAAGAGATCTAATTTCCTATTGTTCTATAATGCAAGGGTTGTCGATCCACGGGCGTGGCGATCAAGCTGTGTCACTCTTCGAGAGGATGCTGGATGAAGGTCTAACTCCCGATGAAGTAGCCTTCACAGTCATCTTGACAGCTTGTAGCCGTGCTGGGTTCGTGGATGAAGGTTGGCATTACTTTGAATTCATGAGGAGTAAATACTCCATGATTCCCTCTCCTGATCACTATGCATGTATTGTTGATCTTCTTAGTCGATCTGGGAGACTAAAAGCAGCTTACGAGCTTATAAAATCCGTGCCTGTTCAATCCCATGCTGGTGCATGGGGTGCACTGCTAGGGGCCTGCAAATTATATTGCAATTCTGAGCTTGCAGAAGTGGTCGCTTCTCGACTTATTGAGCTTGAGCCTCAAAATGCTGGCAACTATGTGCTGTTATCAAATATCTATGCTGCAGCAGAAAGGTGGTTGGATGTGTCTGTCGTAAGAAACCAAATGAATGAGAGAGGGGTTAGAAAAATACCTGGTTGCAGTTGGATT

Coding sequence (CDS)

TGCAAGACCCCCATTGACCTCAAGCAAGTCCATGCCCGTATCGTCCAGAAAGGACTCGAGCAAGATCATTTCCTCGTGACCCAATTCATTTCATCGTCCAATTCATTTGCCAACATCTTCTATTCCACATCCGTCTTTGATCGTGTTGTCTCACCCAGTACCTTTCTTTGGAACTCTCTTGTCCGTGGATACTGTAAGAAGTTGCATTTTGTTGATACGATTTCGCTATTTGTGCGTATGAAAAGGGAGGACGGCGTTCCTGATCGGTATACGTTCCCTTCGCTTCTCAAGGCGTGCGCGAGTGAGGGGAAGGTGATTGAAGGGATGGTCCTTCACGGATCCATCTTGAGATGTGGGGTTGATGGGGATTTATATGTGACGACGAGTTTAGTGAACGTGTATGGGAAGGGTGGGTTAGTCGACTGTGCTCGTAAGGTGTTTGATGGGATGTCGCAGAAGAATGTGAATGTTGTATCTTTCACAACAATGATCGATGGGTATGCTAAAGCCGGCGACATGGTTTCTGCCAGGAAATTATTTCAACAAGCTCCTGAGAGAGATATCGTCGCATGGTCGGCTTTGATATCTGGGTATACACAAAATGGACATCCAAATGAGGCAGTCGAGACATTTCTCGAAATGGGTTTAAGGAATGTGAAACCTGATGAGTTTGTATTGGCAAGCTTGATGTCAGCTTGTTCCCAAGTGGGTAATTTGGATTTAGCTAAATGGGTTGATTCATATGCTACCAAGTTATTGATTGATCTTCGTGGAGCTCACGTTAGGGCAGCTCTTATAGACATGAACGCCAAGTGTGGAAACATGGAGAGAGCCATGAATCTGTTTGATGAAATGCCTAAAAGAGATCTAATTTCCTATTGTTCTATAATGCAAGGGTTGTCGATCCACGGGCGTGGCGATCAAGCTGTGTCACTCTTCGAGAGGATGCTGGATGAAGGTCTAACTCCCGATGAAGTAGCCTTCACAGTCATCTTGACAGCTTGTAGCCGTGCTGGGTTCGTGGATGAAGGTTGGCATTACTTTGAATTCATGAGGAGTAAATACTCCATGATTCCCTCTCCTGATCACTATGCATGTATTGTTGATCTTCTTAGTCGATCTGGGAGACTAAAAGCAGCTTACGAGCTTATAAAATCCGTGCCTGTTCAATCCCATGCTGGTGCATGGGGTGCACTGCTAGGGGCCTGCAAATTATATTGCAATTCTGAGCTTGCAGAAGTGGTCGCTTCTCGACTTATTGAGCTTGAGCCTCAAAATGCTGGCAACTATGTGCTGTTATCAAATATCTATGCTGCAGCAGAAAGGTGGTTGGATGTGTCTGTCGTAAGAAACCAAATGAATGAGAGAGGGGTTAGAAAAATACCTGGTTGCAGTTGGATT

Protein sequence

CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSLVRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLDLAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLIELEPQNAGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI
Homology
BLAST of MS011228 vs. NCBI nr
Match: XP_022132921.1 (putative pentatricopeptide repeat-containing protein At5g37570 [Momordica charantia])

HSP 1 Score: 922.5 bits (2383), Expect = 1.4e-264
Identity = 467/527 (88.61%), Postives = 467/527 (88.61%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL
Sbjct: 47  CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 106

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV
Sbjct: 107 VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 166

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV                         
Sbjct: 167 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNVVSWTAMIVGYSNIGNLVEAKKLFDS 226

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSARKLF
Sbjct: 227 MPERNVASWNALIGGCMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARKLF 286

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD
Sbjct: 287 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 346

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL
Sbjct: 347 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 406

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS
Sbjct: 407 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 466

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI
Sbjct: 467 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 526

BLAST of MS011228 vs. NCBI nr
Match: XP_022972648.1 (putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita maxima])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 420/527 (79.70%), Postives = 443/527 (84.06%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDL+Q+HAR+VQKGLEQDHFLVTQF+S+SNS AN+ YSTSVFDRV+SPSTFLWN+L
Sbjct: 39  CKTPIDLQQLHARLVQKGLEQDHFLVTQFVSTSNSIANVSYSTSVFDRVLSPSTFLWNTL 98

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYC+KL FVDTISLFVRMKRE+GVPDRYTFPSLLKACASEGKV EGM LHGSILRCGV
Sbjct: 99  VRGYCEKLQFVDTISLFVRMKREEGVPDRYTFPSLLKACASEGKVREGMALHGSILRCGV 158

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D DLYVTTSLVN+YGKGGL++CARKVFD MS+KNV                         
Sbjct: 159 DEDLYVTTSLVNLYGKGGLIECARKVFDRMSEKNVVSWTAMIVGYSSTGNLVEAKRLFDS 218

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSAR LF
Sbjct: 219 MPERNVASWNATIGGYMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARNLF 278

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+AP RDIVAWSALISGY QNG PNEAV+TFLEMG RNVKPDEFVL SLMSACSQVGNLD
Sbjct: 279 QKAPGRDIVAWSALISGYAQNGQPNEAVKTFLEMGSRNVKPDEFVLVSLMSACSQVGNLD 338

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKW DSYA K L+DLRGAHVRAALIDMNAKCGNMERAM+LF+EMPKRDLISYCS+MQGL
Sbjct: 339 LAKWADSYACKCLVDLRGAHVRAALIDMNAKCGNMERAMDLFEEMPKRDLISYCSVMQGL 398

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           SIHGRGDQAVSLFERML E LTPDEVAFTVILTACSRAG VDEGWHYFE M SKYSM+PS
Sbjct: 399 SIHGRGDQAVSLFERMLGEALTPDEVAFTVILTACSRAGLVDEGWHYFEIMMSKYSMVPS 458

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVP QSHAGAWGALLGACKLY NSELAEVVASRLI
Sbjct: 459 PDHYACIVDLLSRSGRLKAAYELIKSVPAQSHAGAWGALLGACKLYSNSELAEVVASRLI 518

BLAST of MS011228 vs. NCBI nr
Match: XP_022962851.1 (putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita moschata] >XP_022962852.1 putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita moschata] >XP_022962853.1 putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita moschata] >XP_022962854.1 putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita moschata])

HSP 1 Score: 839.3 bits (2167), Expect = 1.6e-239
Identity = 419/527 (79.51%), Postives = 446/527 (84.63%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDL+Q+HAR+VQKGLEQDHFLVTQF+S+SNS ANI YSTSVFDRV+SPSTFLWN+L
Sbjct: 26  CKTPIDLQQLHARLVQKGLEQDHFLVTQFVSTSNSIANISYSTSVFDRVLSPSTFLWNTL 85

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYC+KL FVDTISLFVRMKRE+GVPDRYTFPSLLKACASEGKV EGM LHGSILRCGV
Sbjct: 86  VRGYCEKLQFVDTISLFVRMKREEGVPDRYTFPSLLKACASEGKVREGMALHGSILRCGV 145

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D DLYVTTSLVN+YGKGGL++CARKVFD MS+KNV                         
Sbjct: 146 DEDLYVTTSLVNLYGKGGLIECARKVFDRMSEKNVVSWTSMIVGYSSTGNLVEAKRLFDS 205

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSAR LF
Sbjct: 206 MPERNVASWNAIIGGYMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARNLF 265

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+APERDIVAWSALISGY QNG PNEAV+TFLEMG RNVKPDEFVL SLMSACSQVGNLD
Sbjct: 266 QKAPERDIVAWSALISGYAQNGQPNEAVKTFLEMGSRNVKPDEFVLVSLMSACSQVGNLD 325

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYA+K L+DLRGAHVRAALIDMNAKCGNMERAM+LF+EMPKRDLISYCS+MQGL
Sbjct: 326 LAKWVDSYASKCLVDLRGAHVRAALIDMNAKCGNMERAMDLFEEMPKRDLISYCSVMQGL 385

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           S+HG GDQAVSLFERML E LTPDEVAFTVILTACSRAG VDEGWHYFE MRSKYSM+PS
Sbjct: 386 SLHGCGDQAVSLFERMLGEALTPDEVAFTVILTACSRAGLVDEGWHYFEIMRSKYSMVPS 445

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVP QSHAGAWGALLGACKLY +SELAEVVASRL+
Sbjct: 446 PDHYACIVDLLSRSGRLKAAYELIKSVPAQSHAGAWGALLGACKLYSDSELAEVVASRLM 505

BLAST of MS011228 vs. NCBI nr
Match: KAG7027181.1 (putative pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 839.3 bits (2167), Expect = 1.6e-239
Identity = 419/527 (79.51%), Postives = 446/527 (84.63%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDL+Q+HAR+VQKGLEQDHFLVTQF+S+SNS ANI YSTSVFDRV+SPSTFLWN+L
Sbjct: 26  CKTPIDLQQLHARLVQKGLEQDHFLVTQFVSTSNSIANISYSTSVFDRVLSPSTFLWNTL 85

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYC+KL FVDTISLFVRMKRE+GVPDRYTFPSLLKACASEGKV EGM LHGSILRCGV
Sbjct: 86  VRGYCEKLQFVDTISLFVRMKREEGVPDRYTFPSLLKACASEGKVREGMALHGSILRCGV 145

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D DLYVTTSLVN+YGKGGL++CARKVFD MS+KNV                         
Sbjct: 146 DEDLYVTTSLVNLYGKGGLIECARKVFDRMSEKNVVSWTAMIVGYSSTGNLVEAKRLFDS 205

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSAR LF
Sbjct: 206 MPERNVASWNAIIGGYMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARNLF 265

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+APERDIVAWSALISGY QNG PNEAV+TFLEMG RNVKPDEFVL SLMSACSQVGNLD
Sbjct: 266 QKAPERDIVAWSALISGYAQNGQPNEAVKTFLEMGSRNVKPDEFVLVSLMSACSQVGNLD 325

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYA+K L+DLRGAHVRAALIDMNAKCGNMERAM+LF+EMPKRDLISYCS+MQGL
Sbjct: 326 LAKWVDSYASKCLVDLRGAHVRAALIDMNAKCGNMERAMDLFEEMPKRDLISYCSVMQGL 385

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           S+HG GDQAVSLFERML E LTPDEVAFTVILTACSRAG VDEGWHYFE MRSKYSM+PS
Sbjct: 386 SLHGCGDQAVSLFERMLGEALTPDEVAFTVILTACSRAGLVDEGWHYFEIMRSKYSMVPS 445

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVP QSHAGAWGALLGACKLY +SELAEVVASRL+
Sbjct: 446 PDHYACIVDLLSRSGRLKAAYELIKSVPAQSHAGAWGALLGACKLYSDSELAEVVASRLM 505

BLAST of MS011228 vs. NCBI nr
Match: KAG6595166.1 (putative pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 839.3 bits (2167), Expect = 1.6e-239
Identity = 419/527 (79.51%), Postives = 446/527 (84.63%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDL+Q+HAR+VQKGLEQDHFLVTQF+S+SNS ANI YSTSVFDRV+SPSTFLWN+L
Sbjct: 27  CKTPIDLQQLHARLVQKGLEQDHFLVTQFVSTSNSIANISYSTSVFDRVLSPSTFLWNTL 86

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYC+KL FVDTISLFVRMKRE+GVPDRYTFPSLLKACASEGKV EGM LHGSILRCGV
Sbjct: 87  VRGYCEKLQFVDTISLFVRMKREEGVPDRYTFPSLLKACASEGKVREGMALHGSILRCGV 146

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D DLYVTTSLVN+YGKGGL++CARKVFD MS+KNV                         
Sbjct: 147 DEDLYVTTSLVNLYGKGGLIECARKVFDRMSEKNVVSWTAMIVGYSSTGNLVEAKRLFDS 206

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSAR LF
Sbjct: 207 MPERNVASWNAIIGGYMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARNLF 266

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+APERDIVAWSALISGY QNG PNEAV+TFLEMG RNVKPDEFVL SLMSACSQVGNLD
Sbjct: 267 QKAPERDIVAWSALISGYAQNGQPNEAVKTFLEMGSRNVKPDEFVLVSLMSACSQVGNLD 326

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYA+K L+DLRGAHVRAALIDMNAKCGNMERAM+LF+EMPKRDLISYCS+MQGL
Sbjct: 327 LAKWVDSYASKCLVDLRGAHVRAALIDMNAKCGNMERAMDLFEEMPKRDLISYCSVMQGL 386

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           S+HG GDQAVSLFERML E LTPDEVAFTVILTACSRAG VDEGWHYFE MRSKYSM+PS
Sbjct: 387 SLHGCGDQAVSLFERMLGEALTPDEVAFTVILTACSRAGLVDEGWHYFEIMRSKYSMVPS 446

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVP QSHAGAWGALLGACKLY +SELAEVVASRL+
Sbjct: 447 PDHYACIVDLLSRSGRLKAAYELIKSVPAQSHAGAWGALLGACKLYSDSELAEVVASRLM 506

BLAST of MS011228 vs. ExPASy Swiss-Prot
Match: Q9FHR3 (Putative pentatricopeptide repeat-containing protein At5g37570 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E37 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.1e-150
Identity = 270/529 (51.04%), Postives = 357/529 (67.49%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFI-SSSNSFANIFYSTSVFDRVVSPSTFLWNS 60
           CK+ I L Q+HARI++KGLEQD  L++ FI SSS+S +++ YS+SVF+RV SP T+LWN 
Sbjct: 20  CKSEIHLNQIHARIIRKGLEQDQNLISIFISSSSSSSSSLSYSSSVFERVPSPGTYLWNH 79

Query: 61  LVRGYCKKLHFVDTISLFVRMKRED-GVPDRYTFPSLLKACASEGKVIEGMVLHGSILRC 120
           L++GY  K  F +T+S+ +RM R     PD YTFP ++K C++ G+V  G  +HG +LR 
Sbjct: 80  LIKGYSNKFLFFETVSILMRMMRTGLARPDEYTFPLVMKVCSNNGQVRVGSSVHGLVLRI 139

Query: 121 GVDGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV----------------------- 180
           G D D+ V TS V+ YGK   +  ARKVF  M ++N                        
Sbjct: 140 GFDKDVVVGTSFVDFYGKCKDLFSARKVFGEMPERNAVSWTALVVAYVKSGELEEAKSMF 199

Query: 181 -------------------------------------NVVSFTTMIDGYAKAGDMVSARK 240
                                                +++S+T+MIDGYAK GDMVSAR 
Sbjct: 200 DLMPERNLGSWNALVDGLVKSGDLVNAKKLFDEMPKRDIISYTSMIDGYAKGGDMVSARD 259

Query: 241 LFQQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGN 300
           LF++A   D+ AWSALI GY QNG PNEA + F EM  +NVKPDEF++  LMSACSQ+G 
Sbjct: 260 LFEEARGVDVRAWSALILGYAQNGQPNEAFKVFSEMCAKNVKPDEFIMVGLMSACSQMGC 319

Query: 301 LDLAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQ 360
            +L + VDSY  + +      +V  ALIDMNAKCG+M+RA  LF+EMP+RDL+SYCS+M+
Sbjct: 320 FELCEKVDSYLHQRMNKFSSHYVVPALIDMNAKCGHMDRAAKLFEEMPQRDLVSYCSMME 379

Query: 361 GLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMI 420
           G++IHG G +A+ LFE+M+DEG+ PDEVAFTVIL  C ++  V+EG  YFE MR KYS++
Sbjct: 380 GMAIHGCGSEAIRLFEKMVDEGIVPDEVAFTVILKVCGQSRLVEEGLRYFELMRKKYSIL 439

Query: 421 PSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASR 468
            SPDHY+CIV+LLSR+G+LK AYELIKS+P ++HA AWG+LLG C L+ N+E+AEVVA  
Sbjct: 440 ASPDHYSCIVNLLSRTGKLKEAYELIKSMPFEAHASAWGSLLGGCSLHGNTEIAEVVARH 499

BLAST of MS011228 vs. ExPASy Swiss-Prot
Match: Q9FFG8 (Pentatricopeptide repeat-containing protein At5g44230 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H17 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.5e-96
Identity = 180/465 (38.71%), Postives = 284/465 (61.08%), Query Frame = 0

Query: 7   LKQVHARIVQKGLEQDHFLVTQFISSSNSFANIF--YSTSVFDRVVSPSTFLWNSLVRGY 66
           +KQ+H  +++KGL+Q  +++T+ I +          Y+  V + V   + FLW +++RGY
Sbjct: 65  IKQIHGHVLRKGLDQSCYILTKLIRTLTKLGVPMDPYARRVIEPVQFRNPFLWTAVIRGY 124

Query: 67  CKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDL 126
             +  F + I+++  M++E+  P  +TF +LLKAC +   +  G   H    R      +
Sbjct: 125 AIEGKFDEAIAMYGCMRKEEITPVSFTFSALLKACGTMKDLNLGRQFHAQTFRLRGFCFV 184

Query: 127 YVTTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAP 186
           YV  +++++Y K   +DCARKVFD M ++  +V+S+T +I  YA+ G+M  A +LF+  P
Sbjct: 185 YVGNTMIDMYVKCESIDCARKVFDEMPER--DVISWTELIAAYARVGNMECAAELFESLP 244

Query: 187 ERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLDLAKW 246
            +D+VAW+A+++G+ QN  P EA+E F  M    ++ DE  +A  +SAC+Q+G    A  
Sbjct: 245 TKDMVAWTAMVTGFAQNAKPQEALEYFDRMEKSGIRADEVTVAGYISACAQLGASKYADR 304

Query: 247 VDSYATKL-LIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGLSIH 306
               A K          + +ALIDM +KCGN+E A+N+F  M  +++ +Y S++ GL+ H
Sbjct: 305 AVQIAQKSGYSPSDHVVIGSALIDMYSKCGNVEEAVNVFMSMNNKNVFTYSSMILGLATH 364

Query: 307 GRGDQAVSLFERMLDE-GLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPSPD 366
           GR  +A+ LF  M+ +  + P+ V F   L ACS +G VD+G   F+ M   + + P+ D
Sbjct: 365 GRAQEALHLFHYMVTQTEIKPNTVTFVGALMACSHSGLVDQGRQVFDSMYQTFGVQPTRD 424

Query: 367 HYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLIEL 426
           HY C+VDLL R+GRL+ A ELIK++ V+ H G WGALLGAC+++ N E+AE+ A  L EL
Sbjct: 425 HYTCMVDLLGRTGRLQEALELIKTMSVEPHGGVWGALLGACRIHNNPEIAEIAAEHLFEL 484

Query: 427 EPQNAGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI 468
           EP   GNY+LLSN+YA+A  W  V  VR  + E+G++K P  SW+
Sbjct: 485 EPDIIGNYILLSNVYASAGDWGGVLRVRKLIKEKGLKKTPAVSWV 527

BLAST of MS011228 vs. ExPASy Swiss-Prot
Match: Q9LTV8 (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 7.6e-96
Identity = 186/532 (34.96%), Postives = 289/532 (54.32%), Query Frame = 0

Query: 7   LKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSLVRGYCK 66
           LKQ+HAR++  GL+   FL+T+ I +S+SF +I ++  VFD +  P  F WN+++RGY +
Sbjct: 37  LKQIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIFPWNAIIRGYSR 96

Query: 67  KLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDLYV 126
             HF D + ++  M+     PD +TFP LLKAC+    +  G  +H  + R G D D++V
Sbjct: 97  NNHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADVFV 156

Query: 127 TTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAPER 186
              L+ +Y K   +  AR VF+G+      +VS+T ++  YA+ G+ + A ++F Q  + 
Sbjct: 157 QNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKM 216

Query: 187 DI---------------------------------------------------------- 246
           D+                                                          
Sbjct: 217 DVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATA 276

Query: 247 ------------VAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQV 306
                       + W+A+ISGY +NG+  EA++ F EM  ++V+PD   + S +SAC+QV
Sbjct: 277 KILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQV 336

Query: 307 GNLDLAKWVDSYATKLLIDLR-GAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCS 366
           G+L+ A+ +  Y  +   D R    + +ALIDM AKCG++E A  +FD    RD++ + +
Sbjct: 337 GSLEQARSMYEYVGR--SDYRDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSA 396

Query: 367 IMQGLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKY 426
           ++ G  +HGR  +A+SL+  M   G+ P++V F  +L AC+ +G V EGW +F  M + +
Sbjct: 397 MIVGYGLHGRAREAISLYRAMERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRM-ADH 456

Query: 427 SMIPSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVV 468
            + P   HYAC++DLL R+G L  AYE+IK +PVQ     WGALL ACK + + EL E  
Sbjct: 457 KINPQQQHYACVIDLLGRAGHLDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGEYA 516

BLAST of MS011228 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 4.9e-95
Identity = 182/470 (38.72%), Postives = 276/470 (58.72%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFIS---SSNSFANIFYSTSVFDRVVSPSTFLW 60
           C    +LKQ+HAR+++ GL QD + +T+F+S   SS S   + Y+  VFD    P TFLW
Sbjct: 24  CSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQIVFDGFDRPDTFLW 83

Query: 61  NSLVRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILR 120
           N ++RG+        ++ L+ RM       + YTFPSLLKAC++     E   +H  I +
Sbjct: 84  NLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQITK 143

Query: 121 CGVDGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSAR 180
            G + D+Y   SL+N Y   G    A  +FD + +   + VS+ ++I GY KAG M  A 
Sbjct: 144 LGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEP--DDVSWNSVIKGYVKAGKMDIAL 203

Query: 181 KLFQQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVG 240
            LF++  E++ ++W+ +ISGY Q     EA++ F EM   +V+PD   LA+ +SAC+Q+G
Sbjct: 204 TLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQLG 263

Query: 241 NLDLAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIM 300
            L+  KW+ SY  K  I +    +   LIDM AKCG ME A+ +F  + K+ + ++ +++
Sbjct: 264 ALEQGKWIHSYLNKTRIRMDSV-LGCVLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALI 323

Query: 301 QGLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSM 360
            G + HG G +A+S F  M   G+ P+ + FT +LTACS  G V+EG   F  M   Y++
Sbjct: 324 SGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSMERDYNL 383

Query: 361 IPSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVAS 420
            P+ +HY CIVDLL R+G L  A   I+ +P++ +A  WGALL AC+++ N EL E +  
Sbjct: 384 KPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGE 443

Query: 421 RLIELEPQNAGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI 468
            LI ++P + G YV  +NI+A  ++W   +  R  M E+GV K+PGCS I
Sbjct: 444 ILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTI 490

BLAST of MS011228 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.4e-94
Identity = 179/461 (38.83%), Postives = 280/461 (60.74%), Query Frame = 0

Query: 8   KQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSLVRGYCKK 67
           + +H   V+  +  D F+    I    S  ++  +  VF  +       WNS++ G+ +K
Sbjct: 151 QSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQK 210

Query: 68  LHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDLYVT 127
                 + LF +M+ ED      T   +L ACA    +  G  +   I    V+ +L + 
Sbjct: 211 GSPDKALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLA 270

Query: 128 TSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAPERD 187
            +++++Y K G ++ A+++FD M +K  + V++TTM+DGYA + D  +AR++    P++D
Sbjct: 271 NAMLDMYTKCGSIEDAKRLFDAMEEK--DNVTWTTMLDGYAISEDYEAAREVLNSMPQKD 330

Query: 188 IVAWSALISGYTQNGHPNEAVETFLEMGL-RNVKPDEFVLASLMSACSQVGNLDLAKWVD 247
           IVAW+ALIS Y QNG PNEA+  F E+ L +N+K ++  L S +SAC+QVG L+L +W+ 
Sbjct: 331 IVAWNALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIH 390

Query: 248 SYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGLSIHGRG 307
           SY  K  I +   HV +ALI M +KCG++E++  +F+ + KRD+  + +++ GL++HG G
Sbjct: 391 SYIKKHGIRM-NFHVTSALIHMYSKCGDLEKSREVFNSVEKRDVFVWSAMIGGLAMHGCG 450

Query: 308 DQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPSPDHYAC 367
           ++AV +F +M +  + P+ V FT +  ACS  G VDE    F  M S Y ++P   HYAC
Sbjct: 451 NEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAESLFHQMESNYGIVPEEKHYAC 510

Query: 368 IVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLIELEPQN 427
           IVD+L RSG L+ A + I+++P+      WGALLGACK++ N  LAE+  +RL+ELEP+N
Sbjct: 511 IVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGACKIHANLNLAEMACTRLLELEPRN 570

Query: 428 AGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI 468
            G +VLLSNIYA   +W +VS +R  M   G++K PGCS I
Sbjct: 571 DGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKKEPGCSSI 608

BLAST of MS011228 vs. ExPASy TrEMBL
Match: A0A6J1BTM3 (putative pentatricopeptide repeat-containing protein At5g37570 OS=Momordica charantia OX=3673 GN=LOC111005644 PE=4 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 6.8e-265
Identity = 467/527 (88.61%), Postives = 467/527 (88.61%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL
Sbjct: 47  CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 106

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV
Sbjct: 107 VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 166

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV                         
Sbjct: 167 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNVVSWTAMIVGYSNIGNLVEAKKLFDS 226

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSARKLF
Sbjct: 227 MPERNVASWNALIGGCMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARKLF 286

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD
Sbjct: 287 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 346

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL
Sbjct: 347 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 406

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS
Sbjct: 407 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 466

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI
Sbjct: 467 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 526

BLAST of MS011228 vs. ExPASy TrEMBL
Match: A0A6J1IAM0 (putative pentatricopeptide repeat-containing protein At5g37570 OS=Cucurbita maxima OX=3661 GN=LOC111471183 PE=4 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.8e-240
Identity = 420/527 (79.70%), Postives = 443/527 (84.06%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDL+Q+HAR+VQKGLEQDHFLVTQF+S+SNS AN+ YSTSVFDRV+SPSTFLWN+L
Sbjct: 39  CKTPIDLQQLHARLVQKGLEQDHFLVTQFVSTSNSIANVSYSTSVFDRVLSPSTFLWNTL 98

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYC+KL FVDTISLFVRMKRE+GVPDRYTFPSLLKACASEGKV EGM LHGSILRCGV
Sbjct: 99  VRGYCEKLQFVDTISLFVRMKREEGVPDRYTFPSLLKACASEGKVREGMALHGSILRCGV 158

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D DLYVTTSLVN+YGKGGL++CARKVFD MS+KNV                         
Sbjct: 159 DEDLYVTTSLVNLYGKGGLIECARKVFDRMSEKNVVSWTAMIVGYSSTGNLVEAKRLFDS 218

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSAR LF
Sbjct: 219 MPERNVASWNATIGGYMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARNLF 278

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+AP RDIVAWSALISGY QNG PNEAV+TFLEMG RNVKPDEFVL SLMSACSQVGNLD
Sbjct: 279 QKAPGRDIVAWSALISGYAQNGQPNEAVKTFLEMGSRNVKPDEFVLVSLMSACSQVGNLD 338

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKW DSYA K L+DLRGAHVRAALIDMNAKCGNMERAM+LF+EMPKRDLISYCS+MQGL
Sbjct: 339 LAKWADSYACKCLVDLRGAHVRAALIDMNAKCGNMERAMDLFEEMPKRDLISYCSVMQGL 398

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           SIHGRGDQAVSLFERML E LTPDEVAFTVILTACSRAG VDEGWHYFE M SKYSM+PS
Sbjct: 399 SIHGRGDQAVSLFERMLGEALTPDEVAFTVILTACSRAGLVDEGWHYFEIMMSKYSMVPS 458

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVP QSHAGAWGALLGACKLY NSELAEVVASRLI
Sbjct: 459 PDHYACIVDLLSRSGRLKAAYELIKSVPAQSHAGAWGALLGACKLYSNSELAEVVASRLI 518

BLAST of MS011228 vs. ExPASy TrEMBL
Match: A0A6J1HI92 (putative pentatricopeptide repeat-containing protein At5g37570 OS=Cucurbita moschata OX=3662 GN=LOC111463222 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.6e-240
Identity = 419/527 (79.51%), Postives = 446/527 (84.63%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKTPIDL+Q+HAR+VQKGLEQDHFLVTQF+S+SNS ANI YSTSVFDRV+SPSTFLWN+L
Sbjct: 26  CKTPIDLQQLHARLVQKGLEQDHFLVTQFVSTSNSIANISYSTSVFDRVLSPSTFLWNTL 85

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           VRGYC+KL FVDTISLFVRMKRE+GVPDRYTFPSLLKACASEGKV EGM LHGSILRCGV
Sbjct: 86  VRGYCEKLQFVDTISLFVRMKREEGVPDRYTFPSLLKACASEGKVREGMALHGSILRCGV 145

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D DLYVTTSLVN+YGKGGL++CARKVFD MS+KNV                         
Sbjct: 146 DEDLYVTTSLVNLYGKGGLIECARKVFDRMSEKNVVSWTSMIVGYSSTGNLVEAKRLFDS 205

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDMVSAR LF
Sbjct: 206 MPERNVASWNAIIGGYMKMGDLKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMVSARNLF 265

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+APERDIVAWSALISGY QNG PNEAV+TFLEMG RNVKPDEFVL SLMSACSQVGNLD
Sbjct: 266 QKAPERDIVAWSALISGYAQNGQPNEAVKTFLEMGSRNVKPDEFVLVSLMSACSQVGNLD 325

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYA+K L+DLRGAHVRAALIDMNAKCGNMERAM+LF+EMPKRDLISYCS+MQGL
Sbjct: 326 LAKWVDSYASKCLVDLRGAHVRAALIDMNAKCGNMERAMDLFEEMPKRDLISYCSVMQGL 385

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           S+HG GDQAVSLFERML E LTPDEVAFTVILTACSRAG VDEGWHYFE MRSKYSM+PS
Sbjct: 386 SLHGCGDQAVSLFERMLGEALTPDEVAFTVILTACSRAGLVDEGWHYFEIMRSKYSMVPS 445

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
           PDHYACIVDLLSRSGRLKAAYELIKSVP QSHAGAWGALLGACKLY +SELAEVVASRL+
Sbjct: 446 PDHYACIVDLLSRSGRLKAAYELIKSVPAQSHAGAWGALLGACKLYSDSELAEVVASRLM 505

BLAST of MS011228 vs. ExPASy TrEMBL
Match: A0A5A7SLV1 (Putative pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001640 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.9e-231
Identity = 404/527 (76.66%), Postives = 435/527 (82.54%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKT IDL+QVH R++QKGLEQD FLVTQFIS+SNS A+I YSTSVFDRV+SPST LWNSL
Sbjct: 47  CKTHIDLQQVHVRLIQKGLEQDTFLVTQFISASNSVAHISYSTSVFDRVLSPSTILWNSL 106

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           V GYC KL FVD ISL+VRMKREDG PDRYTFPSLLK CASEGK++EGM LHGSILRCGV
Sbjct: 107 VSGYCAKLQFVDIISLYVRMKREDGAPDRYTFPSLLKVCASEGKMMEGMALHGSILRCGV 166

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D D+YVTTSLVN+YGKGGL+ CARKVFD MS+KNV                         
Sbjct: 167 DEDIYVTTSLVNLYGKGGLIGCARKVFDRMSEKNVVSWTAMIVGYSSVGNLVEAKRLFDL 226

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDM+SAR LF
Sbjct: 227 MPERNVASWNAIIGGYMKMGDVKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMLSARNLF 286

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+APERDI+AWSALISGYTQNG PNEAV+TFLEM  RNVKPD+FVL SLM ACSQ+GNLD
Sbjct: 287 QKAPERDIIAWSALISGYTQNGQPNEAVQTFLEMSSRNVKPDKFVLTSLMLACSQLGNLD 346

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYAT  L+DLRGAHVRAALIDMNAKCGNMERAM LF+EMPKRDLISYCS+MQGL
Sbjct: 347 LAKWVDSYATGCLVDLRGAHVRAALIDMNAKCGNMERAMYLFEEMPKRDLISYCSVMQGL 406

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           SIHG GDQAVSLFERMLDE LTPD+VAFTVILTACSRAG VDEGWHYFE MR+KYSM+PS
Sbjct: 407 SIHGHGDQAVSLFERMLDEDLTPDDVAFTVILTACSRAGLVDEGWHYFEMMRNKYSMVPS 466

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
            DHYACIVDLLSRSGRLK AYELIKS+PVQSHAGAWGALLGACKLYC+SELAEVVASRLI
Sbjct: 467 LDHYACIVDLLSRSGRLKEAYELIKSLPVQSHAGAWGALLGACKLYCDSELAEVVASRLI 526

BLAST of MS011228 vs. ExPASy TrEMBL
Match: A0A1S3CHC2 (putative pentatricopeptide repeat-containing protein At5g37570 OS=Cucumis melo OX=3656 GN=LOC103500922 PE=4 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.9e-231
Identity = 404/527 (76.66%), Postives = 435/527 (82.54%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSL 60
           CKT IDL+QVH R++QKGLEQD FLVTQFIS+SNS A+I YSTSVFDRV+SPST LWNSL
Sbjct: 47  CKTHIDLQQVHVRLIQKGLEQDTFLVTQFISASNSVAHISYSTSVFDRVLSPSTILWNSL 106

Query: 61  VRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGV 120
           V GYC KL FVD ISL+VRMKREDG PDRYTFPSLLK CASEGK++EGM LHGSILRCGV
Sbjct: 107 VSGYCAKLQFVDIISLYVRMKREDGAPDRYTFPSLLKVCASEGKMMEGMALHGSILRCGV 166

Query: 121 DGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV------------------------- 180
           D D+YVTTSLVN+YGKGGL+ CARKVFD MS+KNV                         
Sbjct: 167 DEDIYVTTSLVNLYGKGGLIGCARKVFDRMSEKNVVSWTAMIVGYSSVGNLVEAKRLFDL 226

Query: 181 -----------------------------------NVVSFTTMIDGYAKAGDMVSARKLF 240
                                              NVVSFTTMIDGYAKAGDM+SAR LF
Sbjct: 227 MPERNVASWNAIIGGYMKMGDVKSAEKVFDEMPEKNVVSFTTMIDGYAKAGDMLSARNLF 286

Query: 241 QQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLD 300
           Q+APERDI+AWSALISGYTQNG PNEAV+TFLEM  RNVKPD+FVL SLM ACSQ+GNLD
Sbjct: 287 QKAPERDIIAWSALISGYTQNGQPNEAVQTFLEMSSRNVKPDKFVLTSLMLACSQLGNLD 346

Query: 301 LAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGL 360
           LAKWVDSYAT  L+DLRGAHVRAALIDMNAKCGNMERAM LF+EMPKRDLISYCS+MQGL
Sbjct: 347 LAKWVDSYATGCLVDLRGAHVRAALIDMNAKCGNMERAMYLFEEMPKRDLISYCSVMQGL 406

Query: 361 SIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPS 420
           SIHG GDQAVSLFERMLDE LTPD+VAFTVILTACSRAG VDEGWHYFE MR+KYSM+PS
Sbjct: 407 SIHGHGDQAVSLFERMLDEDLTPDDVAFTVILTACSRAGLVDEGWHYFEMMRNKYSMVPS 466

Query: 421 PDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLI 468
            DHYACIVDLLSRSGRLK AYELIKS+PVQSHAGAWGALLGACKLYC+SELAEVVASRLI
Sbjct: 467 LDHYACIVDLLSRSGRLKEAYELIKSLPVQSHAGAWGALLGACKLYCDSELAEVVASRLI 526

BLAST of MS011228 vs. TAIR 10
Match: AT5G37570.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 534.6 bits (1376), Expect = 7.7e-152
Identity = 270/529 (51.04%), Postives = 357/529 (67.49%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFI-SSSNSFANIFYSTSVFDRVVSPSTFLWNS 60
           CK+ I L Q+HARI++KGLEQD  L++ FI SSS+S +++ YS+SVF+RV SP T+LWN 
Sbjct: 20  CKSEIHLNQIHARIIRKGLEQDQNLISIFISSSSSSSSSLSYSSSVFERVPSPGTYLWNH 79

Query: 61  LVRGYCKKLHFVDTISLFVRMKRED-GVPDRYTFPSLLKACASEGKVIEGMVLHGSILRC 120
           L++GY  K  F +T+S+ +RM R     PD YTFP ++K C++ G+V  G  +HG +LR 
Sbjct: 80  LIKGYSNKFLFFETVSILMRMMRTGLARPDEYTFPLVMKVCSNNGQVRVGSSVHGLVLRI 139

Query: 121 GVDGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNV----------------------- 180
           G D D+ V TS V+ YGK   +  ARKVF  M ++N                        
Sbjct: 140 GFDKDVVVGTSFVDFYGKCKDLFSARKVFGEMPERNAVSWTALVVAYVKSGELEEAKSMF 199

Query: 181 -------------------------------------NVVSFTTMIDGYAKAGDMVSARK 240
                                                +++S+T+MIDGYAK GDMVSAR 
Sbjct: 200 DLMPERNLGSWNALVDGLVKSGDLVNAKKLFDEMPKRDIISYTSMIDGYAKGGDMVSARD 259

Query: 241 LFQQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGN 300
           LF++A   D+ AWSALI GY QNG PNEA + F EM  +NVKPDEF++  LMSACSQ+G 
Sbjct: 260 LFEEARGVDVRAWSALILGYAQNGQPNEAFKVFSEMCAKNVKPDEFIMVGLMSACSQMGC 319

Query: 301 LDLAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQ 360
            +L + VDSY  + +      +V  ALIDMNAKCG+M+RA  LF+EMP+RDL+SYCS+M+
Sbjct: 320 FELCEKVDSYLHQRMNKFSSHYVVPALIDMNAKCGHMDRAAKLFEEMPQRDLVSYCSMME 379

Query: 361 GLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMI 420
           G++IHG G +A+ LFE+M+DEG+ PDEVAFTVIL  C ++  V+EG  YFE MR KYS++
Sbjct: 380 GMAIHGCGSEAIRLFEKMVDEGIVPDEVAFTVILKVCGQSRLVEEGLRYFELMRKKYSIL 439

Query: 421 PSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASR 468
            SPDHY+CIV+LLSR+G+LK AYELIKS+P ++HA AWG+LLG C L+ N+E+AEVVA  
Sbjct: 440 ASPDHYSCIVNLLSRTGKLKEAYELIKSMPFEAHASAWGSLLGGCSLHGNTEIAEVVARH 499

BLAST of MS011228 vs. TAIR 10
Match: AT5G44230.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-97
Identity = 180/465 (38.71%), Postives = 284/465 (61.08%), Query Frame = 0

Query: 7   LKQVHARIVQKGLEQDHFLVTQFISSSNSFANIF--YSTSVFDRVVSPSTFLWNSLVRGY 66
           +KQ+H  +++KGL+Q  +++T+ I +          Y+  V + V   + FLW +++RGY
Sbjct: 65  IKQIHGHVLRKGLDQSCYILTKLIRTLTKLGVPMDPYARRVIEPVQFRNPFLWTAVIRGY 124

Query: 67  CKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDL 126
             +  F + I+++  M++E+  P  +TF +LLKAC +   +  G   H    R      +
Sbjct: 125 AIEGKFDEAIAMYGCMRKEEITPVSFTFSALLKACGTMKDLNLGRQFHAQTFRLRGFCFV 184

Query: 127 YVTTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAP 186
           YV  +++++Y K   +DCARKVFD M ++  +V+S+T +I  YA+ G+M  A +LF+  P
Sbjct: 185 YVGNTMIDMYVKCESIDCARKVFDEMPER--DVISWTELIAAYARVGNMECAAELFESLP 244

Query: 187 ERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVGNLDLAKW 246
            +D+VAW+A+++G+ QN  P EA+E F  M    ++ DE  +A  +SAC+Q+G    A  
Sbjct: 245 TKDMVAWTAMVTGFAQNAKPQEALEYFDRMEKSGIRADEVTVAGYISACAQLGASKYADR 304

Query: 247 VDSYATKL-LIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGLSIH 306
               A K          + +ALIDM +KCGN+E A+N+F  M  +++ +Y S++ GL+ H
Sbjct: 305 AVQIAQKSGYSPSDHVVIGSALIDMYSKCGNVEEAVNVFMSMNNKNVFTYSSMILGLATH 364

Query: 307 GRGDQAVSLFERMLDE-GLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPSPD 366
           GR  +A+ LF  M+ +  + P+ V F   L ACS +G VD+G   F+ M   + + P+ D
Sbjct: 365 GRAQEALHLFHYMVTQTEIKPNTVTFVGALMACSHSGLVDQGRQVFDSMYQTFGVQPTRD 424

Query: 367 HYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLIEL 426
           HY C+VDLL R+GRL+ A ELIK++ V+ H G WGALLGAC+++ N E+AE+ A  L EL
Sbjct: 425 HYTCMVDLLGRTGRLQEALELIKTMSVEPHGGVWGALLGACRIHNNPEIAEIAAEHLFEL 484

Query: 427 EPQNAGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI 468
           EP   GNY+LLSN+YA+A  W  V  VR  + E+G++K P  SW+
Sbjct: 485 EPDIIGNYILLSNVYASAGDWGGVLRVRKLIKEKGLKKTPAVSWV 527

BLAST of MS011228 vs. TAIR 10
Match: AT3G12770.1 (mitochondrial editing factor 22 )

HSP 1 Score: 352.4 bits (903), Expect = 5.4e-97
Identity = 186/532 (34.96%), Postives = 289/532 (54.32%), Query Frame = 0

Query: 7   LKQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSLVRGYCK 66
           LKQ+HAR++  GL+   FL+T+ I +S+SF +I ++  VFD +  P  F WN+++RGY +
Sbjct: 37  LKQIHARLLVLGLQFSGFLITKLIHASSSFGDITFARQVFDDLPRPQIFPWNAIIRGYSR 96

Query: 67  KLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDLYV 126
             HF D + ++  M+     PD +TFP LLKAC+    +  G  +H  + R G D D++V
Sbjct: 97  NNHFQDALLMYSNMQLARVSPDSFTFPHLLKACSGLSHLQMGRFVHAQVFRLGFDADVFV 156

Query: 127 TTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAPER 186
              L+ +Y K   +  AR VF+G+      +VS+T ++  YA+ G+ + A ++F Q  + 
Sbjct: 157 QNGLIALYAKCRRLGSARTVFEGLPLPERTIVSWTAIVSAYAQNGEPMEALEIFSQMRKM 216

Query: 187 DI---------------------------------------------------------- 246
           D+                                                          
Sbjct: 217 DVKPDWVALVSVLNAFTCLQDLKQGRSIHASVVKMGLEIEPDLLISLNTMYAKCGQVATA 276

Query: 247 ------------VAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQV 306
                       + W+A+ISGY +NG+  EA++ F EM  ++V+PD   + S +SAC+QV
Sbjct: 277 KILFDKMKSPNLILWNAMISGYAKNGYAREAIDMFHEMINKDVRPDTISITSAISACAQV 336

Query: 307 GNLDLAKWVDSYATKLLIDLR-GAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCS 366
           G+L+ A+ +  Y  +   D R    + +ALIDM AKCG++E A  +FD    RD++ + +
Sbjct: 337 GSLEQARSMYEYVGR--SDYRDDVFISSALIDMFAKCGSVEGARLVFDRTLDRDVVVWSA 396

Query: 367 IMQGLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKY 426
           ++ G  +HGR  +A+SL+  M   G+ P++V F  +L AC+ +G V EGW +F  M + +
Sbjct: 397 MIVGYGLHGRAREAISLYRAMERGGVHPNDVTFLGLLMACNHSGMVREGWWFFNRM-ADH 456

Query: 427 SMIPSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVV 468
            + P   HYAC++DLL R+G L  AYE+IK +PVQ     WGALL ACK + + EL E  
Sbjct: 457 KINPQQQHYACVIDLLGRAGHLDQAYEVIKCMPVQPGVTVWGALLSACKKHRHVELGEYA 516

BLAST of MS011228 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 3.5e-96
Identity = 182/470 (38.72%), Postives = 276/470 (58.72%), Query Frame = 0

Query: 1   CKTPIDLKQVHARIVQKGLEQDHFLVTQFIS---SSNSFANIFYSTSVFDRVVSPSTFLW 60
           C    +LKQ+HAR+++ GL QD + +T+F+S   SS S   + Y+  VFD    P TFLW
Sbjct: 24  CSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISSTSSDFLPYAQIVFDGFDRPDTFLW 83

Query: 61  NSLVRGYCKKLHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILR 120
           N ++RG+        ++ L+ RM       + YTFPSLLKAC++     E   +H  I +
Sbjct: 84  NLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPSLLKACSNLSAFEETTQIHAQITK 143

Query: 121 CGVDGDLYVTTSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSAR 180
            G + D+Y   SL+N Y   G    A  +FD + +   + VS+ ++I GY KAG M  A 
Sbjct: 144 LGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEP--DDVSWNSVIKGYVKAGKMDIAL 203

Query: 181 KLFQQAPERDIVAWSALISGYTQNGHPNEAVETFLEMGLRNVKPDEFVLASLMSACSQVG 240
            LF++  E++ ++W+ +ISGY Q     EA++ F EM   +V+PD   LA+ +SAC+Q+G
Sbjct: 204 TLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQNSDVEPDNVSLANALSACAQLG 263

Query: 241 NLDLAKWVDSYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIM 300
            L+  KW+ SY  K  I +    +   LIDM AKCG ME A+ +F  + K+ + ++ +++
Sbjct: 264 ALEQGKWIHSYLNKTRIRMDSV-LGCVLIDMYAKCGEMEEALEVFKNIKKKSVQAWTALI 323

Query: 301 QGLSIHGRGDQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSM 360
            G + HG G +A+S F  M   G+ P+ + FT +LTACS  G V+EG   F  M   Y++
Sbjct: 324 SGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFYSMERDYNL 383

Query: 361 IPSPDHYACIVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVAS 420
            P+ +HY CIVDLL R+G L  A   I+ +P++ +A  WGALL AC+++ N EL E +  
Sbjct: 384 KPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVIWGALLKACRIHKNIELGEEIGE 443

Query: 421 RLIELEPQNAGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI 468
            LI ++P + G YV  +NI+A  ++W   +  R  M E+GV K+PGCS I
Sbjct: 444 ILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKEQGVAKVPGCSTI 490

BLAST of MS011228 vs. TAIR 10
Match: AT2G29760.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 348.2 bits (892), Expect = 1.0e-95
Identity = 179/461 (38.83%), Postives = 280/461 (60.74%), Query Frame = 0

Query: 8   KQVHARIVQKGLEQDHFLVTQFISSSNSFANIFYSTSVFDRVVSPSTFLWNSLVRGYCKK 67
           + +H   V+  +  D F+    I    S  ++  +  VF  +       WNS++ G+ +K
Sbjct: 151 QSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQK 210

Query: 68  LHFVDTISLFVRMKREDGVPDRYTFPSLLKACASEGKVIEGMVLHGSILRCGVDGDLYVT 127
                 + LF +M+ ED      T   +L ACA    +  G  +   I    V+ +L + 
Sbjct: 211 GSPDKALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLA 270

Query: 128 TSLVNVYGKGGLVDCARKVFDGMSQKNVNVVSFTTMIDGYAKAGDMVSARKLFQQAPERD 187
            +++++Y K G ++ A+++FD M +K  + V++TTM+DGYA + D  +AR++    P++D
Sbjct: 271 NAMLDMYTKCGSIEDAKRLFDAMEEK--DNVTWTTMLDGYAISEDYEAAREVLNSMPQKD 330

Query: 188 IVAWSALISGYTQNGHPNEAVETFLEMGL-RNVKPDEFVLASLMSACSQVGNLDLAKWVD 247
           IVAW+ALIS Y QNG PNEA+  F E+ L +N+K ++  L S +SAC+QVG L+L +W+ 
Sbjct: 331 IVAWNALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIH 390

Query: 248 SYATKLLIDLRGAHVRAALIDMNAKCGNMERAMNLFDEMPKRDLISYCSIMQGLSIHGRG 307
           SY  K  I +   HV +ALI M +KCG++E++  +F+ + KRD+  + +++ GL++HG G
Sbjct: 391 SYIKKHGIRM-NFHVTSALIHMYSKCGDLEKSREVFNSVEKRDVFVWSAMIGGLAMHGCG 450

Query: 308 DQAVSLFERMLDEGLTPDEVAFTVILTACSRAGFVDEGWHYFEFMRSKYSMIPSPDHYAC 367
           ++AV +F +M +  + P+ V FT +  ACS  G VDE    F  M S Y ++P   HYAC
Sbjct: 451 NEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAESLFHQMESNYGIVPEEKHYAC 510

Query: 368 IVDLLSRSGRLKAAYELIKSVPVQSHAGAWGALLGACKLYCNSELAEVVASRLIELEPQN 427
           IVD+L RSG L+ A + I+++P+      WGALLGACK++ N  LAE+  +RL+ELEP+N
Sbjct: 511 IVDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGACKIHANLNLAEMACTRLLELEPRN 570

Query: 428 AGNYVLLSNIYAAAERWLDVSVVRNQMNERGVRKIPGCSWI 468
            G +VLLSNIYA   +W +VS +R  M   G++K PGCS I
Sbjct: 571 DGAHVLLSNIYAKLGKWENVSELRKHMRVTGLKKEPGCSSI 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132921.11.4e-26488.61putative pentatricopeptide repeat-containing protein At5g37570 [Momordica charan... [more]
XP_022972648.11.2e-23979.70putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita maxima... [more]
XP_022962851.11.6e-23979.51putative pentatricopeptide repeat-containing protein At5g37570 [Cucurbita moscha... [more]
KAG7027181.11.6e-23979.51putative pentatricopeptide repeat-containing protein, partial [Cucurbita argyros... [more]
KAG6595166.11.6e-23979.51putative pentatricopeptide repeat-containing protein, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
Q9FHR31.1e-15051.04Putative pentatricopeptide repeat-containing protein At5g37570 OS=Arabidopsis th... [more]
Q9FFG81.5e-9638.71Pentatricopeptide repeat-containing protein At5g44230 OS=Arabidopsis thaliana OX... [more]
Q9LTV87.6e-9634.96Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX... [more]
Q9FJY74.9e-9538.72Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX... [more]
O823801.4e-9438.83Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1BTM36.8e-26588.61putative pentatricopeptide repeat-containing protein At5g37570 OS=Momordica char... [more]
A0A6J1IAM05.8e-24079.70putative pentatricopeptide repeat-containing protein At5g37570 OS=Cucurbita maxi... [more]
A0A6J1HI927.6e-24079.51putative pentatricopeptide repeat-containing protein At5g37570 OS=Cucurbita mosc... [more]
A0A5A7SLV14.9e-23176.66Putative pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa... [more]
A0A1S3CHC24.9e-23176.66putative pentatricopeptide repeat-containing protein At5g37570 OS=Cucumis melo O... [more]
Match NameE-valueIdentityDescription
AT5G37570.17.7e-15251.04Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT5G44230.11.1e-9738.71Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G12770.15.4e-9734.96mitochondrial editing factor 22 [more]
AT5G66520.13.5e-9638.72Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G29760.11.0e-9538.83Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 128..155
e-value: 0.0053
score: 16.9
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 158..189
e-value: 1.5E-6
score: 26.0
coord: 291..325
e-value: 1.1E-6
score: 26.4
coord: 326..354
e-value: 0.0025
score: 15.9
coord: 128..155
e-value: 0.0015
score: 16.6
coord: 189..223
e-value: 2.6E-6
score: 25.2
coord: 56..88
e-value: 3.5E-5
score: 21.7
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 52..100
e-value: 4.7E-9
score: 36.3
coord: 289..336
e-value: 6.4E-9
score: 35.9
coord: 187..233
e-value: 4.5E-9
score: 36.4
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 156..182
e-value: 3.5E-6
score: 26.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 53..87
score: 9.920034
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 324..354
score: 8.988323
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 156..186
score: 9.591195
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 187..221
score: 11.651919
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 289..323
score: 11.564229
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 259..467
e-value: 1.4E-35
score: 125.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 134..252
e-value: 3.9E-29
score: 103.3
coord: 2..133
e-value: 9.5E-17
score: 62.9
NoneNo IPR availablePANTHERPTHR24015:SF1943OJ991214_12.4 PROTEINcoord: 1..467
NoneNo IPR availablePANTHERPTHR24015OS07G0578800 PROTEIN-RELATEDcoord: 1..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011228.1MS011228.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding