MS011194 (gene) Bitter gourd (TR) v1

Overview


Name	MS011194
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Germin-like protein
Location	scaffold239: 71470 .. 72108 (-)
RNA-Seq Expression	MS011194
Synteny	MS011194

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCGAAAATGTTCATACCCATTTTCTCATTTCACCTCATGATCATGATCATCACAATCGCCATGGCCTCAGACCCCGACCCACTTCAAGATTTCTGCATACCAAACAACAGGCCCACGCCCACTGGCCCGTGCAAGGACCCGACCGCCGTCACCGCCGACGACTTCGTGTTCTCCGGCGGGAGAGCCGCCGGCAACTTCTCGGGAACCGGGTTCGCCAGCGTGGCCGCAAACCCAGCTGTTTTCCCCGGGCTGAACACGCTGGGAATGTCGTTCGTACGCGCGGACTTGAAGGTGGGCGCGTGGAATCCGCCGCACGTGCACCCACGCGCGGCGGAGATGGTGTACGTGGTCCGGGGGAGGGTGTACGCCGGTTTTGTTGACTCGGCGAACCGGGTTTTCGCGAGGGTGGTGGAGGAGGGGGAGGTGATGGCGGTTCCGAGAGGGCTGCTGCATTTTCAGATGAACGTGGGGACGACGGCGGCGACGGTTTTTGGGTGTTTCAACAGCGAAAATCCGGGAGTCCAGAAGATGGGGTCAGCCATTTTTGGGTCTGGAATTAAAGAGGAGCTTTTGGAGAAGGCTTTTGGGTTGAGTTTGAAGCAGATTAGGAGGTTGAGGAGAAAGTTTCAGTCT

mRNA sequence

ATGTCTCCGAAAATGTTCATACCCATTTTCTCATTTCACCTCATGATCATGATCATCACAATCGCCATGGCCTCAGACCCCGACCCACTTCAAGATTTCTGCATACCAAACAACAGGCCCACGCCCACTGGCCCGTGCAAGGACCCGACCGCCGTCACCGCCGACGACTTCGTGTTCTCCGGCGGGAGAGCCGCCGGCAACTTCTCGGGAACCGGGTTCGCCAGCGTGGCCGCAAACCCAGCTGTTTTCCCCGGGCTGAACACGCTGGGAATGTCGTTCGTACGCGCGGACTTGAAGGTGGGCGCGTGGAATCCGCCGCACGTGCACCCACGCGCGGCGGAGATGGTGTACGTGGTCCGGGGGAGGGTGTACGCCGGTTTTGTTGACTCGGCGAACCGGGTTTTCGCGAGGGTGGTGGAGGAGGGGGAGGTGATGGCGGTTCCGAGAGGGCTGCTGCATTTTCAGATGAACGTGGGGACGACGGCGGCGACGGTTTTTGGGTGTTTCAACAGCGAAAATCCGGGAGTCCAGAAGATGGGGTCAGCCATTTTTGGGTCTGGAATTAAAGAGGAGCTTTTGGAGAAGGCTTTTGGGTTGAGTTTGAAGCAGATTAGGAGGTTGAGGAGAAAGTTTCAGTCT

Coding sequence (CDS)

ATGTCTCCGAAAATGTTCATACCCATTTTCTCATTTCACCTCATGATCATGATCATCACAATCGCCATGGCCTCAGACCCCGACCCACTTCAAGATTTCTGCATACCAAACAACAGGCCCACGCCCACTGGCCCGTGCAAGGACCCGACCGCCGTCACCGCCGACGACTTCGTGTTCTCCGGCGGGAGAGCCGCCGGCAACTTCTCGGGAACCGGGTTCGCCAGCGTGGCCGCAAACCCAGCTGTTTTCCCCGGGCTGAACACGCTGGGAATGTCGTTCGTACGCGCGGACTTGAAGGTGGGCGCGTGGAATCCGCCGCACGTGCACCCACGCGCGGCGGAGATGGTGTACGTGGTCCGGGGGAGGGTGTACGCCGGTTTTGTTGACTCGGCGAACCGGGTTTTCGCGAGGGTGGTGGAGGAGGGGGAGGTGATGGCGGTTCCGAGAGGGCTGCTGCATTTTCAGATGAACGTGGGGACGACGGCGGCGACGGTTTTTGGGTGTTTCAACAGCGAAAATCCGGGAGTCCAGAAGATGGGGTCAGCCATTTTTGGGTCTGGAATTAAAGAGGAGCTTTTGGAGAAGGCTTTTGGGTTGAGTTTGAAGCAGATTAGGAGGTTGAGGAGAAAGTTTCAGTCT

Protein sequence

MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTGPCKDPTAVTADDFVFSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS

Homology

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BLAST of MS011194 vs. NCBI nr
Match: XP_022925793.1 (germin-like protein subfamily 3 member 2 [Cucurbita moschata] >KAG7034505.1 Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322.4 bits (825), Expect = 2.9e-84
Identity = 162/215 (75.35%), Postives = 185/215 (86.05%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTG--PCKDPTAVTADDFV 60
           M  K+ IPIF+FHL   I+T  +ASDPDPLQDFCIPN +PT     PCK+ TAVTADDFV
Sbjct: 3   MCLKILIPIFAFHL---ILTTVVASDPDPLQDFCIPNPKPTKKDDLPCKNSTAVTADDFV 62

Query: 61  FSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYV 120
           FSGG++AG FS  GFA+VAANP++FPGLNTLGMSFVRAD K GA NPPHVHPRAAEMVYV
Sbjct: 63  FSGGKSAGPFSDNGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYV 122

Query: 121 VRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQK 180
           V+GRVYAGFVDSANRV+A+V+ EGEVM +PRGL+HFQMNVG   ATVFGCFNS+NPG Q+
Sbjct: 123 VQGRVYAGFVDSANRVYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQR 182

Query: 181 MGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS 214
           M SAIFGSGIKEELLEKAFGLS K +RR+RR+F+S
Sbjct: 183 MASAIFGSGIKEELLEKAFGLSSKDLRRMRRRFES 214

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BLAST of MS011194 vs. NCBI nr
Match: XP_022977900.1 (germin-like protein subfamily 3 member 2 [Cucurbita maxima])

HSP 1 Score: 320.9 bits (821), Expect = 8.6e-84
Identity = 161/215 (74.88%), Postives = 187/215 (86.98%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTG--PCKDPTAVTADDFV 60
           M  K+ IPIF+FHL   I+TI +ASDPDPLQDFCIPN + T     PCK+ TAVTADDFV
Sbjct: 3   MCLKILIPIFAFHL---ILTIVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFV 62

Query: 61  FSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYV 120
           FSGG++AG FS +GFA+VAANP++FPGLNTLGMSFVRAD K GA NPPHVHPRAAEMVYV
Sbjct: 63  FSGGKSAGPFSESGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYV 122

Query: 121 VRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQK 180
           V+GRVYAGFVDSANR++A+V+ EGEVM +PRGL+HFQMNVG   ATVFGCFNS+NPG Q+
Sbjct: 123 VQGRVYAGFVDSANRMYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQR 182

Query: 181 MGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS 214
           M SAIFGSGIKEELLEKAFGLS K++RR+RR+F+S
Sbjct: 183 MASAIFGSGIKEELLEKAFGLSSKELRRMRRRFES 214

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BLAST of MS011194 vs. NCBI nr
Match: KAG6604355.1 (Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 319.3 bits (817), Expect = 2.5e-83
Identity = 161/215 (74.88%), Postives = 184/215 (85.58%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTG--PCKDPTAVTADDFV 60
           M  K+ IPIF+FHL   I+T  +ASDPDPLQDFCIPN + T     PCK+ TAVTADDFV
Sbjct: 3   MCLKILIPIFAFHL---ILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFV 62

Query: 61  FSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYV 120
           FSGG++AG FS  GFA+VAANP++FPGLNTLGMSFVRAD K GA NPPHVHPRAAEMVYV
Sbjct: 63  FSGGKSAGPFSDNGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYV 122

Query: 121 VRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQK 180
           V+GRVYAGFVDSANRV+A+V+ EGEVM +PRGL+HFQMNVG   ATVFGCFNS+NPG Q+
Sbjct: 123 VQGRVYAGFVDSANRVYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQR 182

Query: 181 MGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS 214
           M SAIFGSGIKEELLEKAFGLS K +RR+RR+F+S
Sbjct: 183 MASAIFGSGIKEELLEKAFGLSSKDLRRMRRRFES 214

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BLAST of MS011194 vs. NCBI nr
Match: XP_023545026.1 (germin-like protein subfamily 3 member 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-81
Identity = 158/215 (73.49%), Postives = 183/215 (85.12%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTG--PCKDPTAVTADDFV 60
           M  K+ IPIF+FHL   I+T  +ASDPDPLQDFCIPN + T     PCK+ TAVTADDFV
Sbjct: 3   MCLKILIPIFAFHL---ILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFV 62

Query: 61  FSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYV 120
           FSGG++AG FS +GFASVAANP++FPGLNTLGMSFV AD K GA NPPHVHPRAAEMVYV
Sbjct: 63  FSGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYV 122

Query: 121 VRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQK 180
           V+GRVYAGFVDSANRV+A+V+ EGEVM +P+GL+HFQMNVG   A VFGCFNS+NPG Q+
Sbjct: 123 VQGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQR 182

Query: 181 MGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS 214
           M SAIFGSGIKEELLEKAFGLS  ++RR+RR+F+S
Sbjct: 183 MASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214

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BLAST of MS011194 vs. NCBI nr
Match: XP_008462565.1 (PREDICTED: germin-like protein subfamily 3 member 2 [Cucumis melo])

HSP 1 Score: 307.8 bits (787), Expect = 7.5e-80
Identity = 151/199 (75.88%), Postives = 173/199 (86.93%), Query Frame = 0


Query: 15  MIMIITIAMASDPDPLQDFCIPNNRPTPTGPCKDPTAVTADDFVFSGGRAAGNFSGTGFA 74
           +++I+TIAMASDPDPLQDFCIPN +  P   CK+ TAVTADDFVFSG ++AG FS TGFA
Sbjct: 10  ILLILTIAMASDPDPLQDFCIPNPKSFPNQLCKNSTAVTADDFVFSGAKSAGPFSETGFA 69

Query: 75  SVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAGFVDSANRV 134
           +VAANP  FPGLNTLGMS VR DLK GA NPPHVHPRAAEMVYVV+G+VYAGFVDS NRV
Sbjct: 70  TVAANPINFPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVQGKVYAGFVDSGNRV 129

Query: 135 FARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGSGIKEELLE 194
           +A+V+EEGEVM +PRG++HFQMNVG   ATVFGCFNS+NPG QKM +AIFGSGIKEELLE
Sbjct: 130 YAKVIEEGEVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMAAAIFGSGIKEELLE 189

Query: 195 KAFGLSLKQIRRLRRKFQS 214
           KAFGLS K+IRR++R F S
Sbjct: 190 KAFGLSSKEIRRMKRMFDS 208

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BLAST of MS011194 vs. ExPASy Swiss-Prot
Match: Q9SR72 (Germin-like protein subfamily 3 member 2 OS=Arabidopsis thaliana OX=3702 GN=At3g10080 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 2.7e-64
Identity = 122/206 (59.22%), Postives = 155/206 (75.24%), Query Frame = 0


Query: 14  LMIMIITIAMASDPDPLQDFCIPNNRPTP--------TGPCKDPTAVTADDFVFSGGRAA 73
           L+   +   +ASDPDP+QDFCIP    +P          PCK+ + VT +DFVFSG + A
Sbjct: 14  LLCFNVCFTLASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGLKTA 73

Query: 74  GNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYA 133
           GNF+ TGFA+V   P  FPGLNTLG+SFVRADLK G+ NPPH HPRA E+ ++V+GRVY+
Sbjct: 74  GNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGRVYS 133

Query: 134 GFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFG 193
           GFVDS N+V+A+V+EEGE+M  P+GL+HFQMNVG   AT+ G  NS+NPG+QK+ S +FG
Sbjct: 134 GFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNVGDVTATIVGGLNSQNPGIQKIPSVVFG 193

Query: 194 SGIKEELLEKAFGLSLKQIRRLRRKF 212
           SGI EELL KAFGLSLKQI  L+++F
Sbjct: 194 SGINEELLMKAFGLSLKQIGTLKKRF 219

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BLAST of MS011194 vs. ExPASy Swiss-Prot
Match: Q6ESF0 (Germin-like protein 2-4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0532500 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 3.4e-59
Identity = 117/208 (56.25%), Postives = 150/208 (72.12%), Query Frame = 0


Query: 14  LMIMIITIAMASDPDPLQDFCIPN---NRPT-----PTGPCKDPTAVTADDFVFSGGRAA 73
           L +++  +A   DPD +QDFC+P+    RP      P  PC+ P  +TA DF FSG RAA
Sbjct: 12  LAVLLPAMAARGDPDAVQDFCVPDAGRGRPVELAMLPAYPCRSPANLTAGDFAFSGVRAA 71

Query: 74  GNFS-GTGFASVAANPAVFPGLNTLGMSFVRADLK-VGAWNPPHVHPRAAEMVYVVRGRV 133
           GNFS  TGFA V+  PA FPGL+TLGMSF RADL   G  NPPH HPRA E   V+ GRV
Sbjct: 72  GNFSPETGFAGVSVTPAQFPGLHTLGMSFARADLSAAGGVNPPHYHPRATETALVLAGRV 131

Query: 134 YAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAI 193
           YAGFVDS  R+FA+V+E+GEVM  PR ++HFQ+NVG T ATV+G FNSENPG+ ++ + +
Sbjct: 132 YAGFVDSGGRLFAKVLEQGEVMVFPRAMVHFQLNVGDTPATVYGAFNSENPGIVRIPATV 191

Query: 194 FGSGIKEELLEKAFGLSLKQIRRLRRKF 212
           FGSGI+E +LE+AFGL+  ++RRL ++F
Sbjct: 192 FGSGIREAVLERAFGLTPAELRRLEKRF 219

[Close]

BLAST of MS011194 vs. ExPASy Swiss-Prot
Match: Q75HJ4 (Germin-like protein 3-8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0804700 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-38
Identity = 95/225 (42.22%), Postives = 126/225 (56.00%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFC---IPNNRPT--------PTGPCKDP 60
           MS     P+      + ++     +DP+P+QDFC   +P             P  PCK  
Sbjct: 1   MSRTSSAPLLVLSAALAVLASTCIADPEPVQDFCVAVVPRAGDAAAAACPAYPGFPCKPA 60

Query: 61  TAVTADDFVFSGGRAAGNFSGT-GFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHV 120
           + V +DDF F+G   A +     GF   AAN   FPGLNTLG+S  R DL  G  NP H 
Sbjct: 61  STVVSDDFFFAGLAVASDTDNRFGFNVTAANAETFPGLNTLGVSIGRVDLAPGGVNPLHS 120

Query: 121 HPRAAEMVYVVRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGC 180
           HPRA E+++VV GRV AGFV +A   +++V+ EGE   VPRG++HFQ NVG  AA V   
Sbjct: 121 HPRATELIHVVAGRVLAGFVSTAGEFYSKVLGEGETFVVPRGMIHFQYNVGGVAAQVITA 180

Query: 181 FNSENPGVQKMGSAIFGSG--IKEELLEKAFGLSLKQIRRLRRKF 212
           FNS+ PGV   GS +FGS   I + +L K+F +  K I+ L+ KF
Sbjct: 181 FNSQMPGVVAAGSTLFGSDPEIPDAVLAKSFQVDAKIIKLLKSKF 225

[Close]

BLAST of MS011194 vs. ExPASy Swiss-Prot
Match: Q9M8X6 (Germin-like protein subfamily 1 member 6 OS=Arabidopsis thaliana OX=3702 GN=At3g04200 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 4.3e-38
Identity = 96/216 (44.44%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 14  LMIMIITIAMAS------DPDPLQDFCI---PNNRPTPTGP-CKDPTAVTADDFVFSG-- 73
           L+  +I +A+A+      DP+PLQDFC+     NR    G  CKDP +VTA+DF +SG  
Sbjct: 9   LVTQVILLALATSFVSCYDPNPLQDFCVAASETNRVFVNGKFCKDPKSVTANDFSYSGLN 68

Query: 74  -GRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVR 133
             R   NF G+   +V  N    PGLNTLG+S  R D   G  NPPH+HPRA E++ V +
Sbjct: 69  IARNTTNFLGSNVTTVDVNK--IPGLNTLGVSLARLDFAQGGQNPPHIHPRATEILVVTK 128

Query: 134 GRVYAGFVDS---ANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQ 193
           G++  GFV S    NR+F +V++ G+V   P GL+HFQMNV  T A  F  F S+NPG  
Sbjct: 129 GKLLVGFVSSNQDNNRLFYKVLKRGDVFVFPIGLIHFQMNVRRTRAVAFAGFGSQNPGTI 188

Query: 194 KMGSAIFGS--GIKEELLEKAFGLSLKQIRRLRRKF 212
           ++  A+FGS   I +E+L KAF L +K +R L   F
Sbjct: 189 RIADAVFGSNPSIPQEVLAKAFQLDVKLVRFLHIVF 222

[Close]

BLAST of MS011194 vs. ExPASy Swiss-Prot
Match: Q10BU2 (Germin-like protein 3-7 OS=Oryza sativa subsp. japonica OX=39947 GN=GER7 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.8e-37
Identity = 87/208 (41.83%), Postives = 126/208 (60.58%), Query Frame = 0


Query: 15  MIMIITIAMASDPDPLQDFCIPNNR-------PTPTGPCKDPTAVTADDFVFSGGRAAGN 74
           ++ ++    A+DP+P+QDFC+   R         P  PCK  +AV +DDF F+G  AAG+
Sbjct: 25  VLAVLASTCAADPEPIQDFCVAVPRAGGEASPAYPGFPCKPASAVVSDDFFFAGLAAAGS 84

Query: 75  FSGTGFASV-AANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAG 134
                 AS+   N   FP LNTLG++  R DL  G  NP H HPRAAE+V+V+ GR+  G
Sbjct: 85  TDNPFGASLKPGNVEAFPALNTLGVAINRVDLAPGGVNPLHSHPRAAELVHVITGRMLVG 144

Query: 135 FVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGS 194
           FV +A + +++VV EGE  A+PRGL+HFQ N G  +A     FNS+ PGV    +A+FG+
Sbjct: 145 FVSTAGKYYSKVVGEGETFAIPRGLMHFQYNPGNASARAMTVFNSQLPGVVPAATALFGA 204

Query: 195 G--IKEELLEKAFGLSLKQIRRLRRKFQ 213
              I + +L K+F +  + I+ L+ KF+
Sbjct: 205 DPEIPDAVLAKSFQVDAEIIKLLKSKFK 232

[Close]

BLAST of MS011194 vs. ExPASy TrEMBL
Match: A0A6J1EG93 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433096 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.4e-84
Identity = 162/215 (75.35%), Postives = 185/215 (86.05%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTG--PCKDPTAVTADDFV 60
           M  K+ IPIF+FHL   I+T  +ASDPDPLQDFCIPN +PT     PCK+ TAVTADDFV
Sbjct: 3   MCLKILIPIFAFHL---ILTTVVASDPDPLQDFCIPNPKPTKKDDLPCKNSTAVTADDFV 62

Query: 61  FSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYV 120
           FSGG++AG FS  GFA+VAANP++FPGLNTLGMSFVRAD K GA NPPHVHPRAAEMVYV
Sbjct: 63  FSGGKSAGPFSDNGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYV 122

Query: 121 VRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQK 180
           V+GRVYAGFVDSANRV+A+V+ EGEVM +PRGL+HFQMNVG   ATVFGCFNS+NPG Q+
Sbjct: 123 VQGRVYAGFVDSANRVYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQR 182

Query: 181 MGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS 214
           M SAIFGSGIKEELLEKAFGLS K +RR+RR+F+S
Sbjct: 183 MASAIFGSGIKEELLEKAFGLSSKDLRRMRRRFES 214

[Close]

BLAST of MS011194 vs. ExPASy TrEMBL
Match: A0A6J1INL7 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111478053 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 4.1e-84
Identity = 161/215 (74.88%), Postives = 187/215 (86.98%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPTPTG--PCKDPTAVTADDFV 60
           M  K+ IPIF+FHL   I+TI +ASDPDPLQDFCIPN + T     PCK+ TAVTADDFV
Sbjct: 3   MCLKILIPIFAFHL---ILTIVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFV 62

Query: 61  FSGGRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYV 120
           FSGG++AG FS +GFA+VAANP++FPGLNTLGMSFVRAD K GA NPPHVHPRAAEMVYV
Sbjct: 63  FSGGKSAGPFSESGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYV 122

Query: 121 VRGRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQK 180
           V+GRVYAGFVDSANR++A+V+ EGEVM +PRGL+HFQMNVG   ATVFGCFNS+NPG Q+
Sbjct: 123 VQGRVYAGFVDSANRMYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQR 182

Query: 181 MGSAIFGSGIKEELLEKAFGLSLKQIRRLRRKFQS 214
           M SAIFGSGIKEELLEKAFGLS K++RR+RR+F+S
Sbjct: 183 MASAIFGSGIKEELLEKAFGLSSKELRRMRRRFES 214

[Close]

BLAST of MS011194 vs. ExPASy TrEMBL
Match: A0A1S3CIT4 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103500894 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.6e-80
Identity = 151/199 (75.88%), Postives = 173/199 (86.93%), Query Frame = 0


Query: 15  MIMIITIAMASDPDPLQDFCIPNNRPTPTGPCKDPTAVTADDFVFSGGRAAGNFSGTGFA 74
           +++I+TIAMASDPDPLQDFCIPN +  P   CK+ TAVTADDFVFSG ++AG FS TGFA
Sbjct: 10  ILLILTIAMASDPDPLQDFCIPNPKSFPNQLCKNSTAVTADDFVFSGAKSAGPFSETGFA 69

Query: 75  SVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAGFVDSANRV 134
           +VAANP  FPGLNTLGMS VR DLK GA NPPHVHPRAAEMVYVV+G+VYAGFVDS NRV
Sbjct: 70  TVAANPINFPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVQGKVYAGFVDSGNRV 129

Query: 135 FARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGSGIKEELLE 194
           +A+V+EEGEVM +PRG++HFQMNVG   ATVFGCFNS+NPG QKM +AIFGSGIKEELLE
Sbjct: 130 YAKVIEEGEVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMAAAIFGSGIKEELLE 189

Query: 195 KAFGLSLKQIRRLRRKFQS 214
           KAFGLS K+IRR++R F S
Sbjct: 190 KAFGLSSKEIRRMKRMFDS 208

[Close]

BLAST of MS011194 vs. ExPASy TrEMBL
Match: A0A0A0KHH1 (Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G452110 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.8e-79
Identity = 150/199 (75.38%), Postives = 172/199 (86.43%), Query Frame = 0


Query: 15  MIMIITIAMASDPDPLQDFCIPNNRPTPTGPCKDPTAVTADDFVFSGGRAAGNFSGTGFA 74
           +++I+TIA+ASDPDPLQDFCIPN +  P   CK+ TAVTADDFVFSG ++AG FSGTGFA
Sbjct: 9   ILLILTIAIASDPDPLQDFCIPNPKSFPDQLCKNSTAVTADDFVFSGAKSAGPFSGTGFA 68

Query: 75  SVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAGFVDSANRV 134
           +VAAN   FPGLNTLGMS VR DLK GA NPPHVHPRAAEMVYVV G+VYAGFVDS NRV
Sbjct: 69  TVAANSINFPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVEGKVYAGFVDSGNRV 128

Query: 135 FARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGSGIKEELLE 194
           +A+V+EEGEVM +PRG++HFQMNVG   ATVFGCFNS+NPG QKM +AIFGSGIKEELLE
Sbjct: 129 YAKVIEEGEVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMAAAIFGSGIKEELLE 188

Query: 195 KAFGLSLKQIRRLRRKFQS 214
           KAFGLS K+IRR++R F S
Sbjct: 189 KAFGLSSKEIRRMKRMFDS 207

[Close]

BLAST of MS011194 vs. ExPASy TrEMBL
Match: A0A5A7SGJ6 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001100 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 9.9e-78
Identity = 147/191 (76.96%), Postives = 165/191 (86.39%), Query Frame = 0


Query: 23  MASDPDPLQDFCIPNNRPTPTGPCKDPTAVTADDFVFSGGRAAGNFSGTGFASVAANPAV 82
           MASDPDPLQDFCIPN +  P   CK+ TAVTADDFVFSG ++AG FS TGFA+VAANP  
Sbjct: 1   MASDPDPLQDFCIPNPKSFPNQLCKNSTAVTADDFVFSGAKSAGPFSETGFATVAANPIN 60

Query: 83  FPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAGFVDSANRVFARVVEEG 142
           FPGLNTLGMS VR DLK GA NPPHVHPRAAEMVYVV+G+VYAGFVDS NRV+A+V+EEG
Sbjct: 61  FPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVQGKVYAGFVDSGNRVYAKVIEEG 120

Query: 143 EVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGSGIKEELLEKAFGLSLK 202
           EVM +PRG++HFQMNVG   ATVFGCFNS+NPG QKM +AIFGSGIKEELLEKAFGLS K
Sbjct: 121 EVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMAAAIFGSGIKEELLEKAFGLSSK 180

Query: 203 QIRRLRRKFQS 214
           +IRR++R F S
Sbjct: 181 EIRRMKRMFDS 191

[Close]

BLAST of MS011194 vs. TAIR 10
Match: AT3G10080.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 1.9e-65
Identity = 122/206 (59.22%), Postives = 155/206 (75.24%), Query Frame = 0


Query: 14  LMIMIITIAMASDPDPLQDFCIPNNRPTP--------TGPCKDPTAVTADDFVFSGGRAA 73
           L+   +   +ASDPDP+QDFCIP    +P          PCK+ + VT +DFVFSG + A
Sbjct: 14  LLCFNVCFTLASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGLKTA 73

Query: 74  GNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYA 133
           GNF+ TGFA+V   P  FPGLNTLG+SFVRADLK G+ NPPH HPRA E+ ++V+GRVY+
Sbjct: 74  GNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGRVYS 133

Query: 134 GFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFG 193
           GFVDS N+V+A+V+EEGE+M  P+GL+HFQMNVG   AT+ G  NS+NPG+QK+ S +FG
Sbjct: 134 GFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNVGDVTATIVGGLNSQNPGIQKIPSVVFG 193

Query: 194 SGIKEELLEKAFGLSLKQIRRLRRKF 212
           SGI EELL KAFGLSLKQI  L+++F
Sbjct: 194 SGINEELLMKAFGLSLKQIGTLKKRF 219

[Close]

BLAST of MS011194 vs. TAIR 10
Match: AT3G04200.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 3.1e-39
Identity = 96/216 (44.44%), Postives = 129/216 (59.72%), Query Frame = 0


Query: 14  LMIMIITIAMAS------DPDPLQDFCI---PNNRPTPTGP-CKDPTAVTADDFVFSG-- 73
           L+  +I +A+A+      DP+PLQDFC+     NR    G  CKDP +VTA+DF +SG  
Sbjct: 9   LVTQVILLALATSFVSCYDPNPLQDFCVAASETNRVFVNGKFCKDPKSVTANDFSYSGLN 68

Query: 74  -GRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVR 133
             R   NF G+   +V  N    PGLNTLG+S  R D   G  NPPH+HPRA E++ V +
Sbjct: 69  IARNTTNFLGSNVTTVDVNK--IPGLNTLGVSLARLDFAQGGQNPPHIHPRATEILVVTK 128

Query: 134 GRVYAGFVDS---ANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQ 193
           G++  GFV S    NR+F +V++ G+V   P GL+HFQMNV  T A  F  F S+NPG  
Sbjct: 129 GKLLVGFVSSNQDNNRLFYKVLKRGDVFVFPIGLIHFQMNVRRTRAVAFAGFGSQNPGTI 188

Query: 194 KMGSAIFGS--GIKEELLEKAFGLSLKQIRRLRRKF 212
           ++  A+FGS   I +E+L KAF L +K +R L   F
Sbjct: 189 RIADAVFGSNPSIPQEVLAKAFQLDVKLVRFLHIVF 222

[Close]

BLAST of MS011194 vs. TAIR 10
Match: AT5G38960.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.2e-37
Identity = 91/204 (44.61%), Postives = 116/204 (56.86%), Query Frame = 0


Query: 19  ITIAMASDPDPLQDFCIPNNRPTPT-----GPCKDPTAVTADDFVFSGGRAA--GNFSGT 78
           +  A+ASDP PLQDFCI  N P          CKDP  VTADDF FSG   A     S  
Sbjct: 17  LPFAIASDPSPLQDFCIGVNTPANALFVNGKFCKDPKLVTADDFYFSGLDKARTTESSPV 76

Query: 79  GFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVRGRVYAGFVDS- 138
           G      N    PGLNTLG+S VR D  +   NPPH HPRA E++ V  G ++ GF  S 
Sbjct: 77  GSNVTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSF 136

Query: 139 -ANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMGSAIFGS--G 198
             NR+F + + +G+V   P GL+HFQ+N+G   A  F   +S+NPGV  +G+ +FGS   
Sbjct: 137 PENRLFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVIIIGNTLFGSKPP 196

Query: 199 IKEELLEKAFGLSLKQIRRLRRKF 212
           I   +L KAF L  K I +L++KF
Sbjct: 197 IDPNVLAKAFQLDPKVIIQLQKKF 220

[Close]

BLAST of MS011194 vs. TAIR 10
Match: AT3G05930.1 (germin-like protein 8 )

HSP 1 Score: 151.0 bits (380), Expect = 1.1e-36
Identity = 88/213 (41.31%), Postives = 126/213 (59.15%), Query Frame = 0


Query: 7   IPIFSFHLMIMIITIAMA-SDPDPLQDFCI---PNNRPTPTGPCKDPTAVTADDFVFSG- 66
           IPIF   +  M++   MA +D + LQDFC+    N       PCKDP  VT +DF F G 
Sbjct: 6   IPIF---VTFMLVAAHMALADTNMLQDFCVADLSNGLKVNGYPCKDPAKVTPEDFYFIGL 65

Query: 67  -GRAAGNFSGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAEMVYVVR 126
              AA   S  G A   AN    PGLNTLG+S  R D   G  NPPH+HPRA+E ++V+ 
Sbjct: 66  ATAAATANSSMGSAVTGANVEKVPGLNTLGVSISRIDYAPGGLNPPHLHPRASEAIFVLE 125

Query: 127 GRVYAGFVDSANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSENPGVQKMG 186
           GR++ GF+ +  ++ ++ V +G+V   P+ LLHFQ N     A+V   F+S+ PG Q +G
Sbjct: 126 GRLFVGFLTTTGKLISKHVNKGDVFVFPKALLHFQQNPNKAPASVLAAFDSQLPGTQVVG 185

Query: 187 SAIFGSG--IKEELLEKAFGLSLKQIRRLRRKF 212
            ++FGS   I ++LL KAFG +  +I++++ KF
Sbjct: 186 PSLFGSNPPIPDDLLAKAFGAAAPEIQKIKGKF 215

[Close]

BLAST of MS011194 vs. TAIR 10
Match: AT5G38940.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 149.8 bits (377), Expect = 2.4e-36
Identity = 93/222 (41.89%), Postives = 126/222 (56.76%), Query Frame = 0


Query: 1   MSPKMFIPIFSFHLMIMIITIAMASDPDPLQDFCIPNNRPT----PTGP-CKDPTAVTAD 60
           M    F+ + S  L+ + + +A+ASDP  LQDFC+  N         G  CKDP  VTAD
Sbjct: 3   MKSLSFLAVLS--LLALTLPLAIASDPSQLQDFCVSANTSANGVFVNGKFCKDPKLVTAD 62

Query: 61  DFVFSGGRAAGNF-SGTGFASVAANPAVFPGLNTLGMSFVRADLKVGAWNPPHVHPRAAE 120
           DF FSG + A    S  G    A N     GLNTLG+S VR D  V   NPPH HPRA E
Sbjct: 63  DFFFSGLQTARPITSPVGSTVTAVNVNNLLGLNTLGISLVRIDYAVNGQNPPHTHPRATE 122

Query: 121 MVYVVRGRVYAGFVDS--ANRVFARVVEEGEVMAVPRGLLHFQMNVGTTAATVFGCFNSE 180
           ++ V +G +  GFV S   NR+F++V+ EG+V   P GL+HFQ N+G   A  F   +S+
Sbjct: 123 ILVVEQGTLLVGFVTSNPDNRLFSKVLNEGDVFVFPEGLIHFQANIGKAPAVAFAALSSQ 182

Query: 181 NPGVQKMGSAIFGS--GIKEELLEKAFGLSLKQIRRLRRKFQ 213
           NPGV  + + +FG+   I   +L KAF L+ + +  L+ KF+
Sbjct: 183 NPGVITIANTVFGANPAINPTILAKAFQLNPRVVMDLQTKFK 222

The following BLAST results are available for this feature:

BLAST of MS011194 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925793.1	2.9e-84	75.35	germin-like protein subfamily 3 member 2 [Cucurbita moschata] >KAG7034505.1 Germ...	[more]
XP_022977900.1	8.6e-84	74.88	germin-like protein subfamily 3 member 2 [Cucurbita maxima]	[more]
KAG6604355.1	2.5e-83	74.88	Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp....	[more]
XP_023545026.1	1.4e-81	73.49	germin-like protein subfamily 3 member 2 [Cucurbita pepo subsp. pepo]	[more]
XP_008462565.1	7.5e-80	75.88	PREDICTED: germin-like protein subfamily 3 member 2 [Cucumis melo]	[more]

BLAST of MS011194 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SR72	2.7e-64	59.22	Germin-like protein subfamily 3 member 2 OS=Arabidopsis thaliana OX=3702 GN=At3g...	[more]
Q6ESF0	3.4e-59	56.25	Germin-like protein 2-4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0532500...	[more]
Q75HJ4	1.5e-38	42.22	Germin-like protein 3-8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0804700...	[more]
Q9M8X6	4.3e-38	44.44	Germin-like protein subfamily 1 member 6 OS=Arabidopsis thaliana OX=3702 GN=At3g...	[more]
Q10BU2	4.8e-37	41.83	Germin-like protein 3-7 OS=Oryza sativa subsp. japonica OX=39947 GN=GER7 PE=2 SV...	[more]

BLAST of MS011194 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EG93	1.4e-84	75.35	Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433096 PE=3 SV=1	[more]
A0A6J1INL7	4.1e-84	74.88	Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111478053 PE=3 SV=1	[more]
A0A1S3CIT4	3.6e-80	75.88	Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103500894 PE=3 SV=1	[more]
A0A0A0KHH1	1.8e-79	75.38	Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G452110 PE=3 SV=1	[more]
A0A5A7SGJ6	9.9e-78	76.96	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G...	[more]

BLAST of MS011194 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10080.1	1.9e-65	59.22	RmlC-like cupins superfamily protein	[more]
AT3G04200.1	3.1e-39	44.44	RmlC-like cupins superfamily protein	[more]
AT5G38960.1	2.2e-37	44.61	RmlC-like cupins superfamily protein	[more]
AT3G05930.1	1.1e-36	41.31	germin-like protein 8	[more]
AT5G38940.1	2.4e-36	41.89	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 170..185 score: 42.88 coord: 137..157 score: 48.73 coord: 107..127 score: 49.72
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 60..205 e-value: 1.4E-41 score: 154.1
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 68..204 e-value: 1.2E-37 score: 128.8
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 25..213 e-value: 1.0E-67 score: 229.4
None	No IPR available	PANTHER	PTHR31238:SF8	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 2	coord: 10..212
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 10..212
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 26..211 e-value: 9.35734E-79 score: 232.101
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 55..212

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS011194.1	MS011194.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0030145	manganese ion binding