MS011004 (gene) Bitter gourd (TR) v1

Overview
NameMS011004
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNAD(P)-binding domain containing protein
Locationscaffold35: 3533097 .. 3534949 (+)
RNA-Seq ExpressionMS011004
SyntenyMS011004
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTTCTTTTCCTTCCCACCGCTACCACTTCCTCCTTCTGCCAATTCAACTCTCAACGTCTCCATTTTTATCCTCTCTGCAAATCCTTTCTCGCCCTTTCCTACTTCTCCTCCAAGAAATCCCCCTCCCTCTCCATTCTCAACTCCAACAAGGTAATTCTTATTACTATTCTATCTCTGGTTGCCTCTGCGAAGTTGTTCTACCACCATTCACTGTTTAGTTACGAGAAAATGCTGGATTTCCATAACTTAACGAGTTGTTTAGGGCGTAAATCTTACTCGCACTCTCAAGTGGGCTATTAGACTCGAGTTTCCTCTCCGCCGATCGCGCATATGATGTTGTGCAAAGGATCGGGAGGCCCCGAGTTTTAAATTTATGTTCATAATGTTCTTCGCGAGGTTTTTATGATCTCTAGTAATCTATTCTAAGCTCGATTTCCTGTTGGCAGATGGAAGGAAGTGAAATTACGGAAGAAGTTGCAGAGAAACAAAATGAGGACAATTTAAACGTGAAGAAAAAGATATTTGTGGCCGGTGCATCGGGAAGTACCGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGTTTTGAAGTCAAGGCCGGGGTTCGAGACGTAAGCAAGGCGAAAACTACTCTTTCCCCTGGCAATCCTGATCTTCAAATTGTAAGTAAGTTTGTCCATTTTAAAAATTCCGATTCCATGGTAATTTGTTAGGTTGCGGAATATCTTGATTTCTTTCAGTTCTTAATATGTTAATAACATTTCAGGTGAAAGCTGATGTGACTGAAGGCTCTGCTAAGTTAGCAGAAGTCATAGGTTCCGATTCAGAAGCGGTAATCTGTGCTACAGGGTTTCGCCCAGGCTGGGATTTATTTGCGCCATGGAAGGTTTGCTCCTTACCATGAAACTATATTTGAAACATCAAACATAATTGTGAAAGTAGGAGCATCATCAAGTTAATGACCAGCTTGCTAATGCCAAGGGTTGAGTAGAGTAGTAAATTTACTCTTGGGTTGGACCTTGTCTCATGGATTTTAGACCCCTTTTGGATCCATTTAGATTAACCATCTGTGATAAAGATTGCTTGAGTTTAAAGTCTCTGTTCAAAGGTTTTGAAGTGCCAAAGAAAAAGTTGGAAAATATCTTGATGTGAATTGTGAAATTACCAGTAACTGCACACAGATTTCAAACAATTTCAGAATTCACATCAGTGAACCATAATATCCAAAGATTTCAAAACCCCAGGAACTTGTGTATGCTGAATATTCAACGCGGTGCTATCGTTACGCCATATTCTTCAAAGGTATAATCTAGAAACGATTACATTGCTGCAGGTTGACAACTATGGCACTGTAAACCTGGTTGAAGCATGTCGTCAGCTGGGCATAAACAGATTCATTCTCATCAGTTCAATTTTAGTCAATGGAGCTGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTTCTGAATGTTTTTGGACTCACCTTGATAGCCAAGCTTCAGGCGGAGCAGCATATCAGGAAATCGGGTATTAACTACACGATTATTAGGCCTGGAGGTTTGAAAAATGATCCTCCCACTGGAAATTTAGTCATGACAGCAGAAGTAATCACATTCTCCCTTCTTCTTGATCATTGCTTCTCTATGATATTTATGTGGCTAACTGAATCGTGGTTGTTTGTAGGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTCGAGGCATTGGTCCATTCTGAAGCCTCTTACAAGGTCGTTGAAATAGTTTCTCGTGCTGATGCACCGAAGCGTACGTATGAGGATCTTTTTGGGTCCATCAAGCAGCGT

mRNA sequence

ATGGCCTTTCTTTTCCTTCCCACCGCTACCACTTCCTCCTTCTGCCAATTCAACTCTCAACGTCTCCATTTTTATCCTCTCTGCAAATCCTTTCTCGCCCTTTCCTACTTCTCCTCCAAGAAATCCCCCTCCCTCTCCATTCTCAACTCCAACAAGATGGAAGGAAGTGAAATTACGGAAGAAGTTGCAGAGAAACAAAATGAGGACAATTTAAACGTGAAGAAAAAGATATTTGTGGCCGGTGCATCGGGAAGTACCGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGTTTTGAAGTCAAGGCCGGGGTTCGAGACGTAAGCAAGGCGAAAACTACTCTTTCCCCTGGCAATCCTGATCTTCAAATTGTGAAAGCTGATGTGACTGAAGGCTCTGCTAAGTTAGCAGAAGTCATAGGTTCCGATTCAGAAGCGGTAATCTGTGCTACAGGGTTTCGCCCAGGCTGGGATTTATTTGCGCCATGGAAGGTTGACAACTATGGCACTGTAAACCTGGTTGAAGCATGTCGTCAGCTGGGCATAAACAGATTCATTCTCATCAGTTCAATTTTAGTCAATGGAGCTGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTTCTGAATGTTTTTGGACTCACCTTGATAGCCAAGCTTCAGGCGGAGCAGCATATCAGGAAATCGGGTATTAACTACACGATTATTAGGCCTGGAGGTTTGAAAAATGATCCTCCCACTGGAAATTTAGTCATGACAGCAGAAGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTCGAGGCATTGGTCCATTCTGAAGCCTCTTACAAGGTCGTTGAAATAGTTTCTCGTGCTGATGCACCGAAGCGTACGTATGAGGATCTTTTTGGGTCCATCAAGCAGCGT

Coding sequence (CDS)

ATGGCCTTTCTTTTCCTTCCCACCGCTACCACTTCCTCCTTCTGCCAATTCAACTCTCAACGTCTCCATTTTTATCCTCTCTGCAAATCCTTTCTCGCCCTTTCCTACTTCTCCTCCAAGAAATCCCCCTCCCTCTCCATTCTCAACTCCAACAAGATGGAAGGAAGTGAAATTACGGAAGAAGTTGCAGAGAAACAAAATGAGGACAATTTAAACGTGAAGAAAAAGATATTTGTGGCCGGTGCATCGGGAAGTACCGGAAAAAGGATTGTGGAGCAGTTGCTGGCAAGGGGTTTTGAAGTCAAGGCCGGGGTTCGAGACGTAAGCAAGGCGAAAACTACTCTTTCCCCTGGCAATCCTGATCTTCAAATTGTGAAAGCTGATGTGACTGAAGGCTCTGCTAAGTTAGCAGAAGTCATAGGTTCCGATTCAGAAGCGGTAATCTGTGCTACAGGGTTTCGCCCAGGCTGGGATTTATTTGCGCCATGGAAGGTTGACAACTATGGCACTGTAAACCTGGTTGAAGCATGTCGTCAGCTGGGCATAAACAGATTCATTCTCATCAGTTCAATTTTAGTCAATGGAGCTGCAATGGGGCAGATTCTCAACCCAGCTTACATCTTTCTGAATGTTTTTGGACTCACCTTGATAGCCAAGCTTCAGGCGGAGCAGCATATCAGGAAATCGGGTATTAACTACACGATTATTAGGCCTGGAGGTTTGAAAAATGATCCTCCCACTGGAAATTTAGTCATGACAGCAGAAGATACTCTTTATGAAGGAAGCATATCAAGAGATTTAGTTGCAGAAGTAGCTGTCGAGGCATTGGTCCATTCTGAAGCCTCTTACAAGGTCGTTGAAATAGTTTCTCGTGCTGATGCACCGAAGCGTACGTATGAGGATCTTTTTGGGTCCATCAAGCAGCGT

Protein sequence

MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYEDLFGSIKQR
Homology
BLAST of MS011004 vs. NCBI nr
Match: XP_022146656.1 (uncharacterized protein At2g34460, chloroplastic [Momordica charantia])

HSP 1 Score: 594.7 bits (1532), Expect = 4.5e-166
Identity = 307/309 (99.35%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE
Sbjct: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAE IGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE
Sbjct: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 309

BLAST of MS011004 vs. NCBI nr
Match: XP_022977850.1 (uncharacterized protein At2g34460, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-148
Identity = 283/309 (91.59%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP A TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNAITSSFFQSNSQRLHFCPLCKSFLAVS--SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTLIAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVM AEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMAAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 307

BLAST of MS011004 vs. NCBI nr
Match: XP_022925949.1 (uncharacterized protein At2g34460, chloroplastic [Cucurbita moschata] >KAG6581421.1 putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034702.1 putative protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-148
Identity = 283/309 (91.59%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP   TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNTITSSFFQSNSQRLHFCPLCKSFLAVS-SSSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTLIAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 308

BLAST of MS011004 vs. NCBI nr
Match: XP_023544344.1 (uncharacterized protein At2g34460, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 533.9 bits (1374), Expect = 9.3e-148
Identity = 281/309 (90.94%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP   TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNTITSSFFQSNSQRLHFCPLCKSFLAVS-SSSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIV+ADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTLIAKLQAE+HIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEKHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 308

BLAST of MS011004 vs. NCBI nr
Match: XP_038881265.1 (uncharacterized protein At2g34460, chloroplastic [Benincasa hispida])

HSP 1 Score: 518.5 bits (1334), Expect = 4.1e-143
Identity = 274/309 (88.67%), Postives = 288/309 (93.20%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP A TSSF    SQRLHF PLCKSFL +S FSS + PS+SIL+S KMEG+EITE
Sbjct: 1   MAFLSLPNAITSSF----SQRLHFCPLCKSFLPVS-FSSNRFPSISILSSTKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ EDNLN+K+KIFVAGA+GSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGN 
Sbjct: 61  EVAEKQIEDNLNMKRKIFVAGATGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNS 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
            LQIVKADVTEGSAKLAE IGSDSEAVICATGFRPGWDLFAPWKVDN+GTVNLVEACR+L
Sbjct: 121 ALQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNFGTVNLVEACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTLIAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMT EDTLYEGSISRDLV EVAVEALVHS+ASYKVVEIVSRADAPKR+Y+
Sbjct: 241 LKNEPPTGNLVMTPEDTLYEGSISRDLVGEVAVEALVHSQASYKVVEIVSRADAPKRSYD 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 304

BLAST of MS011004 vs. ExPASy Swiss-Prot
Match: Q8H124 (Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g34460 PE=1 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 6.2e-102
Identity = 193/276 (69.93%), Postives = 228/276 (82.61%), Query Frame = 0

Query: 32  LALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIV 91
           L L + S+      S    NK   S  T    EK   +N    KK+FVAGA+G TGKRIV
Sbjct: 5   LLLRHSSAVFFSQSSFFTKNKSFRS-FTSIKMEKGEAENAVKTKKVFVAGATGQTGKRIV 64

Query: 92  EQLLARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEVIGSDSEAVICAT 151
           EQLL+RGF VKAGVRDV KAKT+    +P LQIV+ADVTEG  KLAEVIG DS+AVICAT
Sbjct: 65  EQLLSRGFAVKAGVRDVEKAKTSFK-DDPSLQIVRADVTEGPDKLAEVIGDDSQAVICAT 124

Query: 152 GFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNV 211
           GFRPG+D+F PWKVDN+GTVNLV+ACR+ G+ +F+L+SSILVNGAAMGQILNPAY+FLN+
Sbjct: 125 GFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVLVSSILVNGAAMGQILNPAYLFLNL 184

Query: 212 FGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEV 271
           FGLTL+AKLQAE++I+KSGINYTI+RPGGLKNDPPTGN+VM  EDTLYEGSISRDLVAEV
Sbjct: 185 FGLTLVAKLQAEKYIKKSGINYTIVRPGGLKNDPPTGNVVMEPEDTLYEGSISRDLVAEV 244

Query: 272 AVEALVHSEASYKVVEIVSRADAPKRTYEDLFGSIK 308
           AVEAL+  E+S+KVVEIV+RA+APKR+Y+DLF S+K
Sbjct: 245 AVEALLQEESSFKVVEIVARAEAPKRSYKDLFASVK 278

BLAST of MS011004 vs. ExPASy Swiss-Prot
Match: Q8SKU2 (Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 3.8e-27
Identity = 103/310 (33.23%), Postives = 157/310 (50.65%), Query Frame = 0

Query: 19  SQRLHF--YPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKK 78
           S+  HF  YPL  +       SS  S S     ++    S   E + EK +  + N+   
Sbjct: 34  SKYSHFMRYPLTTTLTNNRIRSSSSSSSSIRAQASGSTKSSTAEGIPEKTDSKDDNL--- 93

Query: 79  IFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKA----------KTTLSPGNPDLQIVK 138
           +FVAGA+G  G R V +L+  GF+V+AGVR+  KA          K   + G  +  + K
Sbjct: 94  VFVAGATGKVGSRTVRELIKLGFKVRAGVRNAQKAGALVQSVKQLKLDGASGGGE-AVEK 153

Query: 139 ADVTEGSAKLAEVIGS---DSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQLG 198
            ++ E   + A+ IGS   ++  VICA G   +  +D+  P ++D   T NLV+A     
Sbjct: 154 LEIVECDLEKADQIGSALGNASTVICAIGASEKEIFDITGPCRIDYRATKNLVDAATVAK 213

Query: 199 INRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGL 258
           +N FIL++S+  N   +     PA I LN+F   LI K +AE+ +  SGI YTI+RPGG+
Sbjct: 214 VNHFILVTSLGTNKFGL-----PAAI-LNLFWGVLIWKRKAEEALLASGIPYTIVRPGGM 273

Query: 259 KND----PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASY-KVVEIVSRADAPK 307
           +        T N+ ++ EDTL+ G +S   VAE+      + + SY K+VE+++   AP 
Sbjct: 274 ERPTDAYKETHNVTLSTEDTLFGGQVSNLQVAELMAIMAKNPDLSYCKIVEVIAETTAPL 333

BLAST of MS011004 vs. ExPASy Swiss-Prot
Match: Q8H0U5 (Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.1e-25
Identity = 96/298 (32.21%), Postives = 148/298 (49.66%), Query Frame = 0

Query: 35  SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKK--IFVAGASGSTGKRIVE 94
           S     +S  LS+  S  +  S +  E     +  NLN K++  +FVAGA+G  G R V 
Sbjct: 44  SVSGKSRSFDLSLRASGPIRASSVVTEA----SPTNLNSKEEDLVFVAGATGKVGSRTVR 103

Query: 95  QLLARGFEVKAGVRDVSKAKTTLSP------GNPD--------LQIVKADVTEGSAKLAE 154
           +LL  GF V+AGVR   +A + +         N D        L+IV+ D+ E    +  
Sbjct: 104 ELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVEKLEIVECDL-EKKDSIQP 163

Query: 155 VIGSDSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGA 214
            +G+ S  +IC  G   +   D+  P+++D   T NLV+A     +N FIL++S+  N  
Sbjct: 164 ALGNAS-VIICCIGASEKEISDITGPYRIDYLATKNLVDAATSAKVNNFILVTSLGTNKF 223

Query: 215 AMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKND----PPTGNLVM 274
                  PA I LN+F   L  K +AE+ + +SG+NY I+RPGG++        T NL +
Sbjct: 224 GF-----PAAI-LNLFWGVLCWKRKAEEALIESGLNYAIVRPGGMERPTDAYKETHNLTL 283

Query: 275 TAEDTLYEGSISRDLVAEVAVEALVHSEASY-KVVEIVSRADAPKRTYEDLFGSIKQR 310
             +DTL+ G +S   VAE+      + + S+ K+VE+V+   AP    E L   I  +
Sbjct: 284 ALDDTLFGGQVSNLQVAELLACMAKNPQLSFSKIVEVVAETTAPLTPIEKLLEKIPSK 329

BLAST of MS011004 vs. ExPASy Swiss-Prot
Match: Q10A77 (Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.8e-22
Identity = 83/254 (32.68%), Postives = 130/254 (51.18%), Query Frame = 0

Query: 77  IFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTL-------------SPGNPDLQ 136
           +F+AGA+G  G R V + +  GF V+AGVR   +A + +             SP    L+
Sbjct: 75  VFIAGATGKVGSRAVREFIKLGFRVRAGVRSAQRASSLVQSVEQLKVDDDATSPAE-RLE 134

Query: 137 IVKADV-TEGSAKLAEVIGSDSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQL 196
           IV+ D+  +  + +   IG ++  V+C+ G   +   D+  P+++D   T NLV+A    
Sbjct: 135 IVECDLEKQAQSDIVSAIG-NAAIVVCSIGASEKDILDVTGPYRIDYMATNNLVQAATAA 194

Query: 197 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 256
            +  FIL++S+  N     +I  PA++ LN+F   L  K +AE+ +  SG+ YTI+RPGG
Sbjct: 195 KVEHFILVTSLGTN-----RIGFPAFL-LNLFWGVLCWKRRAEEALIGSGLPYTIVRPGG 254

Query: 257 LKND----PPTGNLVMTAEDTLYEGSISRDLVAE-VAVEALVHSEASYKVVEIVSRADAP 310
           ++        T NLV+  EDT   G +S   VAE +A  A     A  KVVE ++   AP
Sbjct: 255 MERPTDAFKETHNLVVAVEDTYVGGLVSNLQVAELIACIASNRRTAYCKVVEAIAETTAP 314

BLAST of MS011004 vs. ExPASy Swiss-Prot
Match: O07609 (Uncharacterized sugar epimerase YhfK OS=Bacillus subtilis (strain 168) OX=224308 GN=yhfK PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.3e-14
Identity = 64/222 (28.83%), Postives = 100/222 (45.05%), Query Frame = 0

Query: 76  KIFVAGASGSTGKRIVEQLLAR-GFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSA 135
           K+F+ GA+G  G+R+V          ++A VR   + K +L     +  +      EGS 
Sbjct: 2   KVFLIGANGQIGQRLVSLFQDNPDHSIRAMVRK-EEQKASLEAAGAEAVLAN---LEGSP 61

Query: 136 KLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILV- 195
           +         +A+I   G            VD  G    +EA    GI RFI++S++   
Sbjct: 62  EEIAAAAKGCDAIIFTAGSGGSTGYDKTLLVDLDGAAKAIEAAAIAGIKRFIMVSALQAH 121

Query: 196 NGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMT 255
           N     + L P Y          +AK  A++ +  SG+ YTIIRPGGL+N+P TG   ++
Sbjct: 122 NRENWNEALKPYY----------VAKHYADKILEASGLTYTIIRPGGLRNEPGTG--TVS 181

Query: 256 AEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAP 296
           A   L  G ISRD VA+  + +L       +  ++ +  D P
Sbjct: 182 AAKDLERGFISRDDVAKTVIASLDEKNTENRAFDL-TEGDTP 206

BLAST of MS011004 vs. ExPASy TrEMBL
Match: A0A6J1D008 (uncharacterized protein At2g34460, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015805 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.2e-166
Identity = 307/309 (99.35%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE
Sbjct: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAE IGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTL+AKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLMAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE
Sbjct: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 309

BLAST of MS011004 vs. ExPASy TrEMBL
Match: A0A6J1INH1 (uncharacterized protein At2g34460, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478024 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.0e-149
Identity = 283/309 (91.59%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP A TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNAITSSFFQSNSQRLHFCPLCKSFLAVS--SSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTLIAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVM AEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMAAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 307

BLAST of MS011004 vs. ExPASy TrEMBL
Match: A0A6J1EDM3 (uncharacterized protein At2g34460, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111433212 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.0e-149
Identity = 283/309 (91.59%), Postives = 293/309 (94.82%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MAFL LP   TSSF Q NSQRLHF PLCKSFLA+S  SSKK  S+SILNS KMEG+EITE
Sbjct: 1   MAFLSLPNTITSSFFQSNSQRLHFCPLCKSFLAVS-SSSKKFASISILNSYKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVAEKQ+EDNLNVKKKIFVAGASGSTGKRIVEQLLARGF VKAGVRDVSKAKTTLSPGNP
Sbjct: 61  EVAEKQSEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFAVKAGVRDVSKAKTTLSPGNP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
           DLQIVKADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN+GTVNLV+ACR+L
Sbjct: 121 DLQIVKADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNFGTVNLVQACREL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYIFLNV GLTLIAKLQAEQHIRKSGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVLGLTLIAKLQAEQHIRKSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQR 310
           DLFGSIKQR
Sbjct: 301 DLFGSIKQR 308

BLAST of MS011004 vs. ExPASy TrEMBL
Match: A0A5D3CMY6 (DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008920 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.0e-140
Identity = 266/308 (86.36%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1    MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
            MA LFLP A TSSF +FNSQRLHF PLC SFL+LS FSS +  S+SILNSNKMEG+EITE
Sbjct: 828  MASLFLPNAITSSFSKFNSQRLHFCPLCNSFLSLS-FSSNRFSSISILNSNKMEGNEITE 887

Query: 61   EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
            EVA+ Q+E+N NVK+KIFVAGASGSTGK+IVEQLLARGF VKAGVRDVSKAKTTLSP NP
Sbjct: 888  EVAQTQSEENSNVKRKIFVAGASGSTGKKIVEQLLARGFAVKAGVRDVSKAKTTLSPANP 947

Query: 121  DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
             LQIV+ADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN GTVNLVEACR L
Sbjct: 948  ALQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNLGTVNLVEACRGL 1007

Query: 181  GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
            GINRFILISSILVNGAAMGQILNPAYI LN  GL LIAKLQAE+HIR SGINYTIIRPGG
Sbjct: 1008 GINRFILISSILVNGAAMGQILNPAYIILNALGLVLIAKLQAEKHIRNSGINYTIIRPGG 1067

Query: 241  LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
            LKN+PPTGNLVMT EDTLYEGSISRDLVAEVAVEAL+HS+ASYKVVEIVSRADAPKR+YE
Sbjct: 1068 LKNEPPTGNLVMTPEDTLYEGSISRDLVAEVAVEALLHSQASYKVVEIVSRADAPKRSYE 1127

Query: 301  DLFGSIKQ 309
            DLFGS+KQ
Sbjct: 1128 DLFGSVKQ 1134

BLAST of MS011004 vs. ExPASy TrEMBL
Match: A0A5A7U9X6 (NAD(P)-binding Rossmann-fold superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001550 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.0e-140
Identity = 266/308 (86.36%), Postives = 283/308 (91.88%), Query Frame = 0

Query: 1   MAFLFLPTATTSSFCQFNSQRLHFYPLCKSFLALSYFSSKKSPSLSILNSNKMEGSEITE 60
           MA LFLP A TSSF +FNSQRLHF PLC SFL+LS FSS +  S+SILNSNKMEG+EITE
Sbjct: 1   MASLFLPNAITSSFSKFNSQRLHFCPLCNSFLSLS-FSSNRFSSISILNSNKMEGNEITE 60

Query: 61  EVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP 120
           EVA+ Q+E+N NVK+KIFVAGASGSTGK+IVEQLLARGF VKAGVRDVSKAKTTLSP NP
Sbjct: 61  EVAQTQSEENSNVKRKIFVAGASGSTGKKIVEQLLARGFAVKAGVRDVSKAKTTLSPANP 120

Query: 121 DLQIVKADVTEGSAKLAEVIGSDSEAVICATGFRPGWDLFAPWKVDNYGTVNLVEACRQL 180
            LQIV+ADVTEGSAKLAE IGSDSEAVICATGFR GWDLFAPWKVDN GTVNLVEACR L
Sbjct: 121 ALQIVRADVTEGSAKLAEAIGSDSEAVICATGFRRGWDLFAPWKVDNLGTVNLVEACRGL 180

Query: 181 GINRFILISSILVNGAAMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGG 240
           GINRFILISSILVNGAAMGQILNPAYI LN  GL LIAKLQAE+HIR SGINYTIIRPGG
Sbjct: 181 GINRFILISSILVNGAAMGQILNPAYIILNALGLVLIAKLQAEKHIRNSGINYTIIRPGG 240

Query: 241 LKNDPPTGNLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVSRADAPKRTYE 300
           LKN+PPTGNLVMT EDTLYEGSISRDLVAEVAVEAL+HS+ASYKVVEIVSRADAPKR+YE
Sbjct: 241 LKNEPPTGNLVMTPEDTLYEGSISRDLVAEVAVEALLHSQASYKVVEIVSRADAPKRSYE 300

Query: 301 DLFGSIKQ 309
           DLFGS+KQ
Sbjct: 301 DLFGSVKQ 307

BLAST of MS011004 vs. TAIR 10
Match: AT2G34460.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.4e-103
Identity = 193/276 (69.93%), Postives = 228/276 (82.61%), Query Frame = 0

Query: 32  LALSYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIV 91
           L L + S+      S    NK   S  T    EK   +N    KK+FVAGA+G TGKRIV
Sbjct: 5   LLLRHSSAVFFSQSSFFTKNKSFRS-FTSIKMEKGEAENAVKTKKVFVAGATGQTGKRIV 64

Query: 92  EQLLARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEVIGSDSEAVICAT 151
           EQLL+RGF VKAGVRDV KAKT+    +P LQIV+ADVTEG  KLAEVIG DS+AVICAT
Sbjct: 65  EQLLSRGFAVKAGVRDVEKAKTSFK-DDPSLQIVRADVTEGPDKLAEVIGDDSQAVICAT 124

Query: 152 GFRPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMGQILNPAYIFLNV 211
           GFRPG+D+F PWKVDN+GTVNLV+ACR+ G+ +F+L+SSILVNGAAMGQILNPAY+FLN+
Sbjct: 125 GFRPGFDIFTPWKVDNFGTVNLVDACRKQGVEKFVLVSSILVNGAAMGQILNPAYLFLNL 184

Query: 212 FGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNDPPTGNLVMTAEDTLYEGSISRDLVAEV 271
           FGLTL+AKLQAE++I+KSGINYTI+RPGGLKNDPPTGN+VM  EDTLYEGSISRDLVAEV
Sbjct: 185 FGLTLVAKLQAEKYIKKSGINYTIVRPGGLKNDPPTGNVVMEPEDTLYEGSISRDLVAEV 244

Query: 272 AVEALVHSEASYKVVEIVSRADAPKRTYEDLFGSIK 308
           AVEAL+  E+S+KVVEIV+RA+APKR+Y+DLF S+K
Sbjct: 245 AVEALLQEESSFKVVEIVARAEAPKRSYKDLFASVK 278

BLAST of MS011004 vs. TAIR 10
Match: AT3G18890.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 4.3e-26
Identity = 96/298 (32.21%), Postives = 148/298 (49.66%), Query Frame = 0

Query: 35  SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKK--IFVAGASGSTGKRIVE 94
           S     +S  LS+  S  +  S +  E     +  NLN K++  +FVAGA+G  G R V 
Sbjct: 44  SVSGKSRSFDLSLRASGPIRASSVVTEA----SPTNLNSKEEDLVFVAGATGKVGSRTVR 103

Query: 95  QLLARGFEVKAGVRDVSKAKTTLSP------GNPD--------LQIVKADVTEGSAKLAE 154
           +LL  GF V+AGVR   +A + +         N D        L+IV+ D+ E    +  
Sbjct: 104 ELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVEKLEIVECDL-EKKDSIQP 163

Query: 155 VIGSDSEAVICATGF--RPGWDLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGA 214
            +G+ S  +IC  G   +   D+  P+++D   T NLV+A     +N FIL++S+  N  
Sbjct: 164 ALGNAS-VIICCIGASEKEISDITGPYRIDYLATKNLVDAATSAKVNNFILVTSLGTNKF 223

Query: 215 AMGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKND----PPTGNLVM 274
                  PA I LN+F   L  K +AE+ + +SG+NY I+RPGG++        T NL +
Sbjct: 224 GF-----PAAI-LNLFWGVLCWKRKAEEALIESGLNYAIVRPGGMERPTDAYKETHNLTL 283

Query: 275 TAEDTLYEGSISRDLVAEVAVEALVHSEASY-KVVEIVSRADAPKRTYEDLFGSIKQR 310
             +DTL+ G +S   VAE+      + + S+ K+VE+V+   AP    E L   I  +
Sbjct: 284 ALDDTLFGGQVSNLQVAELLACMAKNPQLSFSKIVEVVAETTAPLTPIEKLLEKIPSK 329

BLAST of MS011004 vs. TAIR 10
Match: AT2G37660.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 5.0e-14
Identity = 80/294 (27.21%), Postives = 140/294 (47.62%), Query Frame = 0

Query: 37  FSSKKSPSLSILNSNKMEGS--EITEEVAEKQNEDNLNVKKKIFVAGASGSTGKRIVEQL 96
           F S  S S SI   +K  G    ++  V+     + L V     V GA G TG+ + ++L
Sbjct: 41  FRSLVSDSTSICGPSKFTGKNRRVSVTVSAAATTEPLTV----LVTGAGGRTGQIVYKKL 100

Query: 97  LARGFEVKAGVRDVSKAKTTLSPGNPDLQIVKADVTEGSAKLAEVIGSDSEAVICAT--G 156
             R  +  A  R + + K +    N + ++   D+ + ++    V G D+  ++ +    
Sbjct: 101 KERSEQFVA--RGLVRTKESKEKINGEDEVFIGDIRDTASIAPAVEGIDALVILTSAVPQ 160

Query: 157 FRPGWDLFA-------------PWKVDNYGTVNLVEACRQLGINRFILISSILVNGAAMG 216
            +PG+D                P +VD  G  N ++A +  G+ + +L+ S  + G  + 
Sbjct: 161 MKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAAKAAGVKQIVLVGS--MGGTNIN 220

Query: 217 QILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKN-DPPTGNLVMTAEDTL 276
             LN +    N+    L+ K +AEQ++  SGI YTIIR GGL++ D     L++  +D L
Sbjct: 221 HPLN-SIGNANI----LVWKRKAEQYLADSGIPYTIIRAGGLQDKDGGIRELLVGKDDEL 280

Query: 277 YE---GSISRDLVAEVAVEALVHSEASYKVVEIVSRAD---APKRTYEDLFGSI 307
            E    +I+R  VAEV V+AL   EA +K +++ S+ +    P + ++ LF  +
Sbjct: 281 LETETRTIARADVAEVCVQALQLEEAKFKALDLASKPEGTGTPTKDFKALFTQV 321

BLAST of MS011004 vs. TAIR 10
Match: AT4G31530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 6.1e-12
Identity = 87/293 (29.69%), Postives = 126/293 (43.00%), Query Frame = 0

Query: 26  PLCKSFLAL--SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGAS 85
           PL  S L L  S+F  +   SLS   S+       TEE  +  +  + +  K + V G +
Sbjct: 27  PLFTSPLPLPSSFFLVRNEASLS---SSITPVQAFTEEAVDTSDLAS-SSSKLVLVVGGT 86

Query: 86  GSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP-DLQIVKADVTEGSAKLAEVIGS 145
           G  G+ +V  LL R    +  +RD+ KA       +   LQ+VK D T  +  L   +  
Sbjct: 87  GGVGQLVVASLLKRNIRSRLLLRDLDKATKLFGKQDEYSLQVVKGD-TRNAEDLDPSMFE 146

Query: 146 DSEAVICATGF----RPGW-DLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAA 205
               VIC TG        W +   P KVD  G  NL+ A     + R +L+SS+   G  
Sbjct: 147 GVTHVICTTGTTAFPSKRWNEENTPEKVDWEGVKNLISALPS-SVKRVVLVSSV---GVT 206

Query: 206 MGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNDPPTG--------- 265
               L   +  +N+FG+ L  K   E  +R SG+ +TIIRPG L + P T          
Sbjct: 207 KSNEL--PWSIMNLFGV-LKYKKMGEDFLRDSGLPFTIIRPGRLTDGPYTSYDLNTLLKA 266

Query: 266 -----NLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVS-RADAP 296
                  V+  +     G +SR +VAE  ++AL       K  EI S + D P
Sbjct: 267 TAGERRAVVIGQGDNLVGEVSRLVVAEACIQALDIEFTQGKAYEINSVKGDGP 307

BLAST of MS011004 vs. TAIR 10
Match: AT4G31530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.4e-11
Identity = 85/287 (29.62%), Postives = 123/287 (42.86%), Query Frame = 0

Query: 26  PLCKSFLAL--SYFSSKKSPSLSILNSNKMEGSEITEEVAEKQNEDNLNVKKKIFVAGAS 85
           PL  S L L  S+F  +   SLS   S+       TEE  +  +  + +  K + V G +
Sbjct: 27  PLFTSPLPLPSSFFLVRNEASLS---SSITPVQAFTEEAVDTSDLAS-SSSKLVLVVGGT 86

Query: 86  GSTGKRIVEQLLARGFEVKAGVRDVSKAKTTLSPGNP-DLQIVKADVTEGSAKLAEVIGS 145
           G  G+ +V  LL R    +  +RD+ KA       +   LQ+VK D T  +  L   +  
Sbjct: 87  GGVGQLVVASLLKRNIRSRLLLRDLDKATKLFGKQDEYSLQVVKGD-TRNAEDLDPSMFE 146

Query: 146 DSEAVICATGF----RPGW-DLFAPWKVDNYGTVNLVEACRQLGINRFILISSILVNGAA 205
               VIC TG        W +   P KVD  G  NL+ A     + R +L+SS+   G  
Sbjct: 147 GVTHVICTTGTTAFPSKRWNEENTPEKVDWEGVKNLISALPS-SVKRVVLVSSV---GVT 206

Query: 206 MGQILNPAYIFLNVFGLTLIAKLQAEQHIRKSGINYTIIRPGGLKNDPPTG--------- 265
               L   +  +N+FG+ L  K   E  +R SG+ +TIIRPG L + P T          
Sbjct: 207 KSNEL--PWSIMNLFGV-LKYKKMGEDFLRDSGLPFTIIRPGRLTDGPYTSYDLNTLLKA 266

Query: 266 -----NLVMTAEDTLYEGSISRDLVAEVAVEALVHSEASYKVVEIVS 291
                  V+  +     G +SR +VAE  ++AL       K  EI S
Sbjct: 267 TAGERRAVVIGQGDNLVGEVSRLVVAEACIQALDIEFTQGKAYEINS 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146656.14.5e-16699.35uncharacterized protein At2g34460, chloroplastic [Momordica charantia][more]
XP_022977850.11.4e-14891.59uncharacterized protein At2g34460, chloroplastic-like [Cucurbita maxima][more]
XP_022925949.11.4e-14891.59uncharacterized protein At2g34460, chloroplastic [Cucurbita moschata] >KAG658142... [more]
XP_023544344.19.3e-14890.94uncharacterized protein At2g34460, chloroplastic-like [Cucurbita pepo subsp. pep... [more]
XP_038881265.14.1e-14388.67uncharacterized protein At2g34460, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8H1246.2e-10269.93Uncharacterized protein At2g34460, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q8SKU23.8e-2733.23Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2[more]
Q8H0U56.1e-2532.21Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1[more]
Q10A772.8e-2232.68Protein TIC 62, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=TIC62 ... [more]
O076096.3e-1428.83Uncharacterized sugar epimerase YhfK OS=Bacillus subtilis (strain 168) OX=224308... [more]
Match NameE-valueIdentityDescription
A0A6J1D0082.2e-16699.35uncharacterized protein At2g34460, chloroplastic OS=Momordica charantia OX=3673 ... [more]
A0A6J1INH17.0e-14991.59uncharacterized protein At2g34460, chloroplastic-like OS=Cucurbita maxima OX=366... [more]
A0A6J1EDM37.0e-14991.59uncharacterized protein At2g34460, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
A0A5D3CMY62.0e-14086.36DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008920 ... [more]
A0A5A7U9X62.0e-14086.36NAD(P)-binding Rossmann-fold superfamily protein OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT2G34460.14.4e-10369.93NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G18890.14.3e-2632.21NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37660.15.0e-1427.21NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G31530.16.1e-1229.69NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G31530.21.4e-1129.62NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 55..300
e-value: 1.6E-49
score: 170.5
NoneNo IPR availablePANTHERPTHR15020:SF39SUBFAMILY NOT NAMEDcoord: 53..309
NoneNo IPR availablePANTHERPTHR15020FLAVIN REDUCTASE-RELATEDcoord: 53..309
NoneNo IPR availableCDDcd05243SDR_a5coord: 76..291
e-value: 1.85419E-58
score: 184.747
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 81..278
e-value: 2.1E-44
score: 151.6
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 75..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS011004.1MS011004.1mRNA