MS010988 (gene) Bitter gourd (TR) v1

Overview


Name	MS010988
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	WRKY protein
Location	scaffold35: 3454528 .. 3455154 (+)
RNA-Seq Expression	MS010988
Synteny	MS010988

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATCGAAGAAGAAGGGAGAGAAGAAGGAGAGAGAGCCGAGATTTGCTTTCATGACAAAGAGCGAGGTGGATCATTTGGAGGATGGATATAGATGGAGAAAATATGGACAAAAAGCTGTCAAGAACAGCGCTTATCCCAGGTAATTTGATTCTCTAATTTCATGTAATTTTAAATTAAACTAATTAAAAGGCCATAAATAAATTAATTTTAATTTTCTGTTTGGTTCTTTTAGAAGCTATTACAGATGCACGACTCAGAAATGTGGGGTGAAGAAACGAGTGGAGAGATCATTTGAAGATCCATCCATTGTGATTACGACGTACGAAGGACAACACAACCACCCAATTCCGGCGACGTTAAGGGGAAACCTGTCGGTTGCCTCCGCGTTTCCGCCGTCCATGCTGACGCCAATGCCGGTGGTCGGTGCTGCCAGGTTTCTTCCAGATCAACTATTGTTGAGTAATAATAATAATGCTTCTGCAGGCCATAACCACGTCGCCGGCGCCGCCGCCTCCGCTTATTCACAAAGCAGCCTCGGCTATTCTTATGGCGGCCGGCAGCCGGAGTATGGGCTTCTGCAGGACATTTTTCCGGCTCCGTCGTCGTCGTTCTTTAACCGGCAGCCG

mRNA sequence

AAATCGAAGAAGAAGGGAGAGAAGAAGGAGAGAGAGCCGAGATTTGCTTTCATGACAAAGAGCGAGGTGGATCATTTGGAGGATGGATATAGATGGAGAAAATATGGACAAAAAGCTGTCAAGAACAGCGCTTATCCCAGAAGCTATTACAGATGCACGACTCAGAAATGTGGGGTGAAGAAACGAGTGGAGAGATCATTTGAAGATCCATCCATTGTGATTACGACGTACGAAGGACAACACAACCACCCAATTCCGGCGACGTTAAGGGGAAACCTGTCGGTTGCCTCCGCGTTTCCGCCGTCCATGCTGACGCCAATGCCGGTGGTCGGTGCTGCCAGGTTTCTTCCAGATCAACTATTGTTGAGTAATAATAATAATGCTTCTGCAGGCCATAACCACGTCGCCGGCGCCGCCGCCTCCGCTTATTCACAAAGCAGCCTCGGCTATTCTTATGGCGGCCGGCAGCCGGAGTATGGGCTTCTGCAGGACATTTTTCCGGCTCCGTCGTCGTCGTTCTTTAACCGGCAGCCG

Coding sequence (CDS)

AAATCGAAGAAGAAGGGAGAGAAGAAGGAGAGAGAGCCGAGATTTGCTTTCATGACAAAGAGCGAGGTGGATCATTTGGAGGATGGATATAGATGGAGAAAATATGGACAAAAAGCTGTCAAGAACAGCGCTTATCCCAGAAGCTATTACAGATGCACGACTCAGAAATGTGGGGTGAAGAAACGAGTGGAGAGATCATTTGAAGATCCATCCATTGTGATTACGACGTACGAAGGACAACACAACCACCCAATTCCGGCGACGTTAAGGGGAAACCTGTCGGTTGCCTCCGCGTTTCCGCCGTCCATGCTGACGCCAATGCCGGTGGTCGGTGCTGCCAGGTTTCTTCCAGATCAACTATTGTTGAGTAATAATAATAATGCTTCTGCAGGCCATAACCACGTCGCCGGCGCCGCCGCCTCCGCTTATTCACAAAGCAGCCTCGGCTATTCTTATGGCGGCCGGCAGCCGGAGTATGGGCTTCTGCAGGACATTTTTCCGGCTCCGTCGTCGTCGTTCTTTAACCGGCAGCCG

Protein sequence

KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVKKRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQLLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP

Homology

[Close]

BLAST of MS010988 vs. NCBI nr
Match: XP_022142261.1 (probable WRKY transcription factor 71 [Momordica charantia])

HSP 1 Score: 358.2 bits (918), Expect = 4.0e-95
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 116 KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 175

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL 120
           KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL
Sbjct: 176 KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL 235

Query: 121 LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP
Sbjct: 236 LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 293

[Close]

BLAST of MS010988 vs. NCBI nr
Match: XP_008439664.1 (PREDICTED: probable WRKY transcription factor 71 [Cucumis melo])

HSP 1 Score: 277.7 bits (709), Expect = 6.9e-71
Identity = 145/179 (81.01%), Postives = 154/179 (86.03%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEK+ERE R AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 90  KSKKKGEKREREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 149

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNHPIPATLRGNLS AS  FPPSMLTPMPVVG   FLP +
Sbjct: 150 KRVERSYEDPSIVITTYEGQHNHPIPATLRGNLSAASGTFPPSMLTPMPVVGGVGFLPAE 209

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           LL    +NAS+ +N   G  A+ YS +S  Y+Y GRQPEYGLLQDIFPAP SSFFNRQP
Sbjct: 210 LL----SNASS-NNQAVGGGATVYSHNSFDYTYNGRQPEYGLLQDIFPAP-SSFFNRQP 262

[Close]

BLAST of MS010988 vs. NCBI nr
Match: XP_038883206.1 (WRKY transcription factor 71 [Benincasa hispida])

HSP 1 Score: 276.2 bits (705), Expect = 2.0e-70
Identity = 144/178 (80.90%), Postives = 152/178 (85.39%), Query Frame = 0


Query: 2   SKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVKK 61
           SKKKGEKKERE R AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVKK
Sbjct: 90  SKKKGEKKEREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVKK 149

Query: 62  RVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQL 121
           RVERSFEDPSIVITTYEGQHNHPIPATLRGNLS AS AF PSML PMPVVG   FLP +L
Sbjct: 150 RVERSFEDPSIVITTYEGQHNHPIPATLRGNLSAASGAFQPSMLAPMPVVGGVGFLPAEL 209

Query: 122 LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           L    +NAS+ +N   G +AS YS S+  Y+Y GRQ E+GLLQDIFPAP SSFFNRQP
Sbjct: 210 L----SNASSNNNQAVGGSASIYSHSNFDYTYNGRQSEFGLLQDIFPAP-SSFFNRQP 262

[Close]

BLAST of MS010988 vs. NCBI nr
Match: KGN49371.1 (hypothetical protein Csa_003326 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.7e-69
Identity = 142/179 (79.33%), Postives = 151/179 (84.36%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEKKERE R AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 90  KSKKKGEKKEREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 149

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNH IPATLRGNLS AS  F PSMLTPMPVVG   FLP +
Sbjct: 150 KRVERSYEDPSIVITTYEGQHNHLIPATLRGNLSAASGTFSPSMLTPMPVVGGVGFLPAE 209

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           LL      ++AG+N   G  A+ YS ++  Y+Y GRQPEYGLLQDIFPAP SSFFNRQP
Sbjct: 210 LL------SNAGNNQAVGGGATVYSHNNFDYTYNGRQPEYGLLQDIFPAP-SSFFNRQP 261

[Close]

BLAST of MS010988 vs. NCBI nr
Match: NP_001292668.1 (probable WRKY transcription factor 71 [Cucumis sativus] >ADU52524.1 WRKY protein [Cucumis sativus])

HSP 1 Score: 269.2 bits (687), Expect = 2.5e-68
Identity = 140/179 (78.21%), Postives = 150/179 (83.80%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEKKERE   AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 90  KSKKKGEKKEREAGVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 149

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNH IPATLRGNLS AS  F PSMLTPMPVVG   FLP +
Sbjct: 150 KRVERSYEDPSIVITTYEGQHNHLIPATLRGNLSAASGTFSPSMLTPMPVVGGVGFLPAE 209

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           L      +++AG+N   G  A+ YS ++  Y+Y GRQPEYGLLQDIFPAP SSFFNRQP
Sbjct: 210 L------SSNAGNNQAVGGGATVYSHNNFDYTYNGRQPEYGLLQDIFPAP-SSFFNRQP 261

[Close]

BLAST of MS010988 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 2.3e-45
Identity = 105/189 (55.56%), Postives = 122/189 (64.55%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KK   KK+REPR +FMTKSEVDHLEDGYRWRKYGQKAVKNS YPRSYYRCTTQKC VK
Sbjct: 146 KTKKTEVKKQREPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 205

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL 120
           KRVERSF+DP++VITTYEGQHNHPIP  LRG+ + A+ F   ++TP        F  D  
Sbjct: 206 KRVERSFQDPTVVITTYEGQHNHPIPTNLRGSSAAAAMFSADLMTP------RSFAHDMF 265

Query: 121 LLSNNNNASAGHNHVAGAAASAYSQSSLG-----------YSYGGRQPEYGLLQDIFPAP 179
             +   N  +    VA A    Y QS  G           Y  GG   EY LL++IFP  
Sbjct: 266 RTAAYTNGGS----VAAALDYGYGQSGYGSVNSNPSSHQVYHQGG---EYELLREIFP-- 318

[Close]

BLAST of MS010988 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 6.8e-45
Identity = 102/175 (58.29%), Postives = 121/175 (69.14%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           + KKKGEKKERE R AFMTKSE+DHLEDGYRWRKYGQKAVKNS YPRSYYRCTTQKC VK
Sbjct: 110 QGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 169

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRG-----NLSVASAFPPSMLTPMPVVGAARF 120
           KRVERSF+DPSIVITTYEG+HNHPIP+TLRG     +L V      S+L   P     R 
Sbjct: 170 KRVERSFQDPSIVITTYEGKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFP-----RH 229

Query: 121 LPDQLLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQP--EYGLLQDIFPA 169
             D L++ +    S  +    G+ +  +   +  Y++   QP  +YGLLQDI P+
Sbjct: 230 HQDFLMMKH----SPANYQSVGSLSYEHGHGTSSYNFNNNQPVVDYGLLQDIVPS 275

[Close]

BLAST of MS010988 vs. ExPASy Swiss-Prot
Match: Q9FL26 (WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.8e-37
Identity = 92/184 (50.00%), Postives = 123/184 (66.85%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KKK EKK +EPR +FMTK+EVDHLEDGYRWRKYGQKAVKNS YPRSYYRCTTQKC VK
Sbjct: 158 KTKKKEEKK-KEPRVSFMTKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 217

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNL---SVASAFPPSMLTPMPVVGAARFLP 120
           KRVERS++DP++VITTYE QHNHPIP   R  +   + AS + PS         ++    
Sbjct: 218 KRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFSGTTASDYNPS---------SSPIFS 277

Query: 121 DQLLLSNNNNASAGHNHVAGAAAS---AYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFF 179
           D ++ +  + ++     V  A+ +   +Y Q   G+    ++ E+ LL+++FP   S FF
Sbjct: 278 DLIINTPRSFSNDDLFRVPYASVNVNPSYHQQQHGFHQ--QESEFELLKEMFP---SVFF 326

[Close]

BLAST of MS010988 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 91/188 (48.40%), Postives = 113/188 (60.11%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KKK +KK RE RFAF+TKS++D+L+DGYRWRKYGQKAVKNS YPRSYYRCTT  CGVK
Sbjct: 195 KAKKKNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVK 254

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSV----------ASAFPPSMLTPMPVV 120
           KRVERS +DPSIV+TTYEGQH HP P T RG++ +           +A   S   P P  
Sbjct: 255 KRVERSSDDPSIVMTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRY 314

Query: 121 GAARFLPDQLLLSNN-----NNASAGHNHVAGAAASA-----YSQSSLGYSYGGRQPEYG 169
              +      + +NN     N  S+    V    +S+     Y  S    S      ++G
Sbjct: 315 LLTQHHQPYNMYNNNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSIRDHG 374

[Close]

BLAST of MS010988 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.0e-33
Identity = 87/185 (47.03%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KK  +K++RE R AFMTKSEVDHLEDGYRWRKYGQKAVKNS +PRSYYRCTT  C VK
Sbjct: 148 KAKKNNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVK 207

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLR--------GNLSVASAFPPSMLTPMPVVGA 120
           KRVERSF DPS V+TTYEGQH H  P T R        G+   AS+         P+ G+
Sbjct: 208 KRVERSFRDPSTVVTTYEGQHTHISPLTSRPISTGGFFGSSGAASSLGNGCF-GFPIDGS 267

Query: 121 ARFLP--DQLLLSNNN-------NASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQ 169
               P   QL+  ++        +   G N    + A+ Y   +          + GLLQ
Sbjct: 268 TLISPQFQQLVQYHHQQQQQELMSCFGGVNEYLNSHANEYGDDNRVKKSRVLVKDNGLLQ 327

[Close]

BLAST of MS010988 vs. ExPASy TrEMBL
Match: A0A6J1CLP0 (probable WRKY transcription factor 71 OS=Momordica charantia OX=3673 GN=LOC111012424 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.0e-95
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 116 KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 175

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL 120
           KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL
Sbjct: 176 KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL 235

Query: 121 LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP
Sbjct: 236 LLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 293

[Close]

BLAST of MS010988 vs. ExPASy TrEMBL
Match: A0A1S3AZZ5 (probable WRKY transcription factor 71 OS=Cucumis melo OX=3656 GN=LOC103484390 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.4e-71
Identity = 145/179 (81.01%), Postives = 154/179 (86.03%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEK+ERE R AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 90  KSKKKGEKREREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 149

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNHPIPATLRGNLS AS  FPPSMLTPMPVVG   FLP +
Sbjct: 150 KRVERSYEDPSIVITTYEGQHNHPIPATLRGNLSAASGTFPPSMLTPMPVVGGVGFLPAE 209

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           LL    +NAS+ +N   G  A+ YS +S  Y+Y GRQPEYGLLQDIFPAP SSFFNRQP
Sbjct: 210 LL----SNASS-NNQAVGGGATVYSHNSFDYTYNGRQPEYGLLQDIFPAP-SSFFNRQP 262

[Close]

BLAST of MS010988 vs. ExPASy TrEMBL
Match: A0A0A0KNS1 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G522650 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 8.3e-70
Identity = 142/179 (79.33%), Postives = 151/179 (84.36%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEKKERE R AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 90  KSKKKGEKKEREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 149

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNH IPATLRGNLS AS  F PSMLTPMPVVG   FLP +
Sbjct: 150 KRVERSYEDPSIVITTYEGQHNHLIPATLRGNLSAASGTFSPSMLTPMPVVGGVGFLPAE 209

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           LL      ++AG+N   G  A+ YS ++  Y+Y GRQPEYGLLQDIFPAP SSFFNRQP
Sbjct: 210 LL------SNAGNNQAVGGGATVYSHNNFDYTYNGRQPEYGLLQDIFPAP-SSFFNRQP 261

[Close]

BLAST of MS010988 vs. ExPASy TrEMBL
Match: E7CEY1 (WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY46 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.2e-68
Identity = 140/179 (78.21%), Postives = 150/179 (83.80%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKKGEKKERE   AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 90  KSKKKGEKKEREAGVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 149

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNH IPATLRGNLS AS  F PSMLTPMPVVG   FLP +
Sbjct: 150 KRVERSYEDPSIVITTYEGQHNHLIPATLRGNLSAASGTFSPSMLTPMPVVGGVGFLPAE 209

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           L      +++AG+N   G  A+ YS ++  Y+Y GRQPEYGLLQDIFPAP SSFFNRQP
Sbjct: 210 L------SSNAGNNQAVGGGATVYSHNNFDYTYNGRQPEYGLLQDIFPAP-SSFFNRQP 261

[Close]

BLAST of MS010988 vs. ExPASy TrEMBL
Match: A0A6J1ED06 (probable WRKY transcription factor 71 OS=Cucurbita moschata OX=3662 GN=LOC111433209 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 6.6e-67
Identity = 141/180 (78.33%), Postives = 147/180 (81.67%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           KSKKK EKKEREPR AFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK
Sbjct: 93  KSKKKVEKKEREPRVAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 152

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVAS-AFPPSMLTPMPVVGAARFLPDQ 120
           KRVERS+EDPSIVITTYEGQHNHPIPATLRGNLS AS AFPPSML PMPVVG  R+LP Q
Sbjct: 153 KRVERSYEDPSIVITTYEGQHNHPIPATLRGNLSAASGAFPPSMLAPMPVVGGVRYLP-Q 212

Query: 121 LLLSNNNNASAGHNHVAGAAASAYSQ-SSLGYSYGGRQPEYGLLQDIFPAPSSSFFNRQP 179
           L+    NN S  +N   G   S YSQ SS  Y Y GRQ +YGLLQDIFP  +  F NRQP
Sbjct: 213 LM----NNTSVNNNQAVGGGESVYSQSSSFNYPYNGRQQDYGLLQDIFPT-APPFLNRQP 266

[Close]

BLAST of MS010988 vs. TAIR 10
Match: AT4G18170.1 (WRKY DNA-binding protein 28 )

HSP 1 Score: 183.3 bits (464), Expect = 1.7e-46
Identity = 105/189 (55.56%), Postives = 122/189 (64.55%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KK   KK+REPR +FMTKSEVDHLEDGYRWRKYGQKAVKNS YPRSYYRCTTQKC VK
Sbjct: 146 KTKKTEVKKQREPRVSFMTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 205

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSVASAFPPSMLTPMPVVGAARFLPDQL 120
           KRVERSF+DP++VITTYEGQHNHPIP  LRG+ + A+ F   ++TP        F  D  
Sbjct: 206 KRVERSFQDPTVVITTYEGQHNHPIPTNLRGSSAAAAMFSADLMTP------RSFAHDMF 265

Query: 121 LLSNNNNASAGHNHVAGAAASAYSQSSLG-----------YSYGGRQPEYGLLQDIFPAP 179
             +   N  +    VA A    Y QS  G           Y  GG   EY LL++IFP  
Sbjct: 266 RTAAYTNGGS----VAAALDYGYGQSGYGSVNSNPSSHQVYHQGG---EYELLREIFP-- 318

[Close]

BLAST of MS010988 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 181.8 bits (460), Expect = 4.8e-46
Identity = 102/175 (58.29%), Postives = 121/175 (69.14%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           + KKKGEKKERE R AFMTKSE+DHLEDGYRWRKYGQKAVKNS YPRSYYRCTTQKC VK
Sbjct: 110 QGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 169

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRG-----NLSVASAFPPSMLTPMPVVGAARF 120
           KRVERSF+DPSIVITTYEG+HNHPIP+TLRG     +L V      S+L   P     R 
Sbjct: 170 KRVERSFQDPSIVITTYEGKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFP-----RH 229

Query: 121 LPDQLLLSNNNNASAGHNHVAGAAASAYSQSSLGYSYGGRQP--EYGLLQDIFPA 169
             D L++ +    S  +    G+ +  +   +  Y++   QP  +YGLLQDI P+
Sbjct: 230 HQDFLMMKH----SPANYQSVGSLSYEHGHGTSSYNFNNNQPVVDYGLLQDIVPS 275

[Close]

BLAST of MS010988 vs. TAIR 10
Match: AT5G46350.1 (WRKY DNA-binding protein 8 )

HSP 1 Score: 157.1 bits (396), Expect = 1.3e-38
Identity = 92/184 (50.00%), Postives = 123/184 (66.85%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KKK EKK +EPR +FMTK+EVDHLEDGYRWRKYGQKAVKNS YPRSYYRCTTQKC VK
Sbjct: 158 KTKKKEEKK-KEPRVSFMTKTEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 217

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNL---SVASAFPPSMLTPMPVVGAARFLP 120
           KRVERS++DP++VITTYE QHNHPIP   R  +   + AS + PS         ++    
Sbjct: 218 KRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFSGTTASDYNPS---------SSPIFS 277

Query: 121 DQLLLSNNNNASAGHNHVAGAAAS---AYSQSSLGYSYGGRQPEYGLLQDIFPAPSSSFF 179
           D ++ +  + ++     V  A+ +   +Y Q   G+    ++ E+ LL+++FP   S FF
Sbjct: 278 DLIINTPRSFSNDDLFRVPYASVNVNPSYHQQQHGFHQ--QESEFELLKEMFP---SVFF 326

[Close]

BLAST of MS010988 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 154.5 bits (389), Expect = 8.3e-38
Identity = 91/188 (48.40%), Postives = 113/188 (60.11%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KKK +KK RE RFAF+TKS++D+L+DGYRWRKYGQKAVKNS YPRSYYRCTT  CGVK
Sbjct: 195 KAKKKNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVK 254

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLRGNLSV----------ASAFPPSMLTPMPVV 120
           KRVERS +DPSIV+TTYEGQH HP P T RG++ +           +A   S   P P  
Sbjct: 255 KRVERSSDDPSIVMTTYEGQHTHPFPMTPRGHIGMLTSPILDHGATTASSSSFSIPQPRY 314

Query: 121 GAARFLPDQLLLSNN-----NNASAGHNHVAGAAASA-----YSQSSLGYSYGGRQPEYG 169
              +      + +NN     N  S+    V    +S+     Y  S    S      ++G
Sbjct: 315 LLTQHHQPYNMYNNNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSIRDHG 374

[Close]

BLAST of MS010988 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 142.1 bits (357), Expect = 4.2e-34
Identity = 87/185 (47.03%), Postives = 107/185 (57.84%), Query Frame = 0


Query: 1   KSKKKGEKKEREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSAYPRSYYRCTTQKCGVK 60
           K+KK  +K++RE R AFMTKSEVDHLEDGYRWRKYGQKAVKNS +PRSYYRCTT  C VK
Sbjct: 148 KAKKNNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVK 207

Query: 61  KRVERSFEDPSIVITTYEGQHNHPIPATLR--------GNLSVASAFPPSMLTPMPVVGA 120
           KRVERSF DPS V+TTYEGQH H  P T R        G+   AS+         P+ G+
Sbjct: 208 KRVERSFRDPSTVVTTYEGQHTHISPLTSRPISTGGFFGSSGAASSLGNGCF-GFPIDGS 267

Query: 121 ARFLP--DQLLLSNNN-------NASAGHNHVAGAAASAYSQSSLGYSYGGRQPEYGLLQ 169
               P   QL+  ++        +   G N    + A+ Y   +          + GLLQ
Sbjct: 268 TLISPQFQQLVQYHHQQQQQELMSCFGGVNEYLNSHANEYGDDNRVKKSRVLVKDNGLLQ 327

The following BLAST results are available for this feature:

BLAST of MS010988 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142261.1	4.0e-95	100.00	probable WRKY transcription factor 71 [Momordica charantia]	[more]
XP_008439664.1	6.9e-71	81.01	PREDICTED: probable WRKY transcription factor 71 [Cucumis melo]	[more]
XP_038883206.1	2.0e-70	80.90	WRKY transcription factor 71 [Benincasa hispida]	[more]
KGN49371.1	1.7e-69	79.33	hypothetical protein Csa_003326 [Cucumis sativus]	[more]
NP_001292668.1	2.5e-68	78.21	probable WRKY transcription factor 71 [Cucumis sativus] >ADU52524.1 WRKY protein...	[more]

BLAST of MS010988 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VWJ2	2.3e-45	55.56	WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1	[more]
Q93WV4	6.8e-45	58.29	WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1	[more]
Q9FL26	1.8e-37	50.00	WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1	[more]
Q9FGZ4	1.2e-36	48.40	Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ...	[more]
O22900	6.0e-33	47.03	WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1	[more]

BLAST of MS010988 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLP0	2.0e-95	100.00	probable WRKY transcription factor 71 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A1S3AZZ5	3.4e-71	81.01	probable WRKY transcription factor 71 OS=Cucumis melo OX=3656 GN=LOC103484390 PE...	[more]
A0A0A0KNS1	8.3e-70	79.33	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G522650 PE=4 S...	[more]
E7CEY1	1.2e-68	78.21	WRKY protein OS=Cucumis sativus OX=3659 GN=WRKY46 PE=2 SV=1	[more]
A0A6J1ED06	6.6e-67	78.33	probable WRKY transcription factor 71 OS=Cucurbita moschata OX=3662 GN=LOC111433...	[more]

BLAST of MS010988 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18170.1	1.7e-46	55.56	WRKY DNA-binding protein 28	[more]
AT1G29860.1	4.8e-46	58.29	WRKY DNA-binding protein 71	[more]
AT5G46350.1	1.3e-38	50.00	WRKY DNA-binding protein 8	[more]
AT5G49520.1	8.3e-38	48.40	WRKY DNA-binding protein 48	[more]
AT2G47260.1	4.2e-34	47.03	WRKY DNA-binding protein 23	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 26..85 e-value: 1.6E-39 score: 147.3
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 27..84 e-value: 3.9E-26 score: 91.1
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 21..86 score: 31.456171
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 11..87 e-value: 1.9E-35 score: 122.5
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 18..86
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 1..169
None	No IPR available	PANTHER	PTHR31221:SF177	WRKY TRANSCRIPTION FACTOR 28-RELATED	coord: 1..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010988.1	MS010988.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding