MS010974 (gene) Bitter gourd (TR) v1

BLAST of MS010974 vs. NCBI nr
Match: XP_022142067.1 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase [Momordica charantia])

HSP 1 Score: 749.2 bits (1933), Expect = 1.7e-212
Identity = 368/370 (99.46%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTD+DPLIVPVPDGESEPSVPVVDI
Sbjct: 3   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDRDPLIVPVPDGESEPSVPVVDI 62

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL
Sbjct: 63  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 122

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST
Sbjct: 123 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 182

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA
Sbjct: 183 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 242

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNET+GLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK
Sbjct: 243 LTILYQLNETKGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 302

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM
Sbjct: 303 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 362

Query: 361 KNVGDQDHVC 371
           KNVGDQDHVC
Sbjct: 363 KNVGDQDHVC 372

BLAST of MS010974 vs. NCBI nr
Match: XP_038883132.1 (protein SRG1-like [Benincasa hispida] >XP_038883136.1 protein SRG1-like [Benincasa hispida])

HSP 1 Score: 588.6 bits (1516), Expect = 3.8e-164
Identity = 292/370 (78.92%), Postives = 324/370 (87.57%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   +FG+S+IVPSV ELAK  I KIPLRYQR DQDP I  VP GES P VPVVD+
Sbjct: 1   MESNTEMVDFGKSIIVPSVLELAKQSIPKIPLRYQRADQDPPI--VPGGESGPPVPVVDL 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
            RL +GDSAS   E+ KLHSAC EWGFFQ++NHGVPTSLL+EFR E+ESFFNLPY EKKL
Sbjct: 61  RRLAIGDSAS--PEIDKLHSACKEWGFFQIINHGVPTSLLDEFRREIESFFNLPYDEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN E+QEGFGQLFVVS+EQKLDWSDMF +TTLPL+LRNPH+F KLPPKLRETLEAYS 
Sbjct: 121 LWQNSESQEGFGQLFVVSDEQKLDWSDMFKITTLPLSLRNPHLFHKLPPKLRETLEAYSI 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           E+KKLA++IL  LA ALKMD EEMRELFRDG Q++RMNYYPPCP+PDKAIGFS HSDADA
Sbjct: 181 EIKKLAIVILDHLAGALKMDVEEMRELFRDGVQSVRMNYYPPCPEPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R  GRW+AVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVS NCSK
Sbjct: 241 LTILYQLNEAEGLQIRKGGRWVAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSLNCSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATFYSSNL+SELGPA SL+GP+N A+FRRV LEKY K+FFARKLEGKSYLE+MR+
Sbjct: 301 ERLSVATFYSSNLNSELGPAKSLVGPHNPAVFRRVMLEKYFKDFFARKLEGKSYLEHMRI 360

Query: 361 KNVGDQDHVC 371
           +N G  DHVC
Sbjct: 361 ENEG--DHVC 364

BLAST of MS010974 vs. NCBI nr
Match: XP_022978722.1 (protein SRG1-like [Cucurbita maxima])

HSP 1 Score: 588.2 bits (1515), Expect = 5.0e-164
Identity = 290/370 (78.38%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   NFG S+IVPSV ELAK P+ KIPLRY+R DQDP +  V D +S PSVP+VD+
Sbjct: 1   MESNTEIVNFGTSIIVPSVLELAKQPLAKIPLRYERADQDPPM--VSDRDSGPSVPIVDL 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRL +GD  SAA+EL KLHSAC EWGFFQ++NH VPTSLLEEFR+E+ESFFNLPY EKKL
Sbjct: 61  HRLAVGD--SAASELDKLHSACKEWGFFQIINHEVPTSLLEEFRMEIESFFNLPYAEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN +NQEGFGQLFVVSEEQKLDWSDMF++TTLPL+LR P +FQKLPPKLRETLEAYST
Sbjct: 121 LWQNSQNQEGFGQLFVVSEEQKLDWSDMFYITTLPLDLREPRLFQKLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLA++IL  LA ALKMD EEMRELFRDG Q+MR+NYYPPCP+PDKAIGFS HSDADA
Sbjct: 181 EVKKLAIVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRW+AVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVS N SK
Sbjct: 241 LTILYQLNEAEGLQIRKDGRWVAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSLNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATF+SSNL+S+LGPANSL+GP N A+FRRV LEKY K+FFARKLEGKSYLE MR+
Sbjct: 301 ERLSVATFHSSNLNSDLGPANSLVGPQNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRI 360

Query: 361 KNVGDQDHVC 371
           +N+G  DH C
Sbjct: 361 ENMG--DHAC 364

BLAST of MS010974 vs. NCBI nr
Match: XP_022925778.1 (protein SRG1-like [Cucurbita moschata] >KAG6581393.1 Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585.9 bits (1509), Expect = 2.5e-163
Identity = 290/370 (78.38%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   NFG S+IVPSV ELAK P+ KIPLRY+R DQDP +  V D +S PSVPVVD+
Sbjct: 1   MESNTEIVNFGTSIIVPSVLELAKQPLSKIPLRYERADQDPPM--VSDRDSGPSVPVVDL 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRL +GDSA AA+EL KLHSAC EWGFFQ++NH V TSLLEEFR+E+ESFFNLPY EKKL
Sbjct: 61  HRLAVGDSA-AASELDKLHSACKEWGFFQIINHEVSTSLLEEFRMEIESFFNLPYAEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN +NQEGFGQLFVVSEEQKLDWSDMF++TTLPL+LR P +FQKLPPKLRETLEAYST
Sbjct: 121 LWQNSQNQEGFGQLFVVSEEQKLDWSDMFYITTLPLDLREPRLFQKLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLA++IL  LA ALKMD EEMRELFRDG Q+MR+NYYPPCP+PDKAIGFS HSDADA
Sbjct: 181 EVKKLAIVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRW++VKPLPNAFV NIGDIMEIVSNGVYKSIEHRVS N SK
Sbjct: 241 LTILYQLNEAEGLQIRKDGRWVSVKPLPNAFVANIGDIMEIVSNGVYKSIEHRVSLNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATF+SSNL+SELGPANSL+GP+N A+FRRV LEKY K+FFARKLEGKSYLE MR+
Sbjct: 301 ERLSVATFHSSNLNSELGPANSLVGPHNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRI 360

Query: 361 KNVGDQDHVC 371
           +N+G  DH C
Sbjct: 361 ENMG--DHAC 365

BLAST of MS010974 vs. NCBI nr
Match: XP_023544157.1 (protein SRG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.9 bits (1504), Expect = 9.4e-163
Identity = 289/370 (78.11%), Postives = 324/370 (87.57%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   NFG S+IVPSV ELAK P+ KIPLRY+R DQDP   P+   +S PSVPVVD+
Sbjct: 1   MESNTEIVNFGTSIIVPSVLELAKQPLAKIPLRYKRADQDP---PMVSDDSGPSVPVVDL 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRL +GD  SAA+EL KLHSAC EWGFFQ++NH V TSLLEEFR+E+ESFFNLPY EK L
Sbjct: 61  HRLAVGD--SAASELDKLHSACKEWGFFQIINHEVSTSLLEEFRMEIESFFNLPYAEKNL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN +NQEGFGQLFVVSEEQKLDWSDMF++TTLPL+LR P +FQKLPPKLRETLEAYST
Sbjct: 121 LWQNSQNQEGFGQLFVVSEEQKLDWSDMFYITTLPLDLREPRLFQKLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLA++IL  LA ALKMD EEMRELFRDG Q+MR+NYYPPCP+PDKAIGFS HSDADA
Sbjct: 181 EVKKLAIVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRW+AVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVS N SK
Sbjct: 241 LTILYQLNEAEGLQIRKDGRWVAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSLNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATF+SSNL+SELGPANSL+GP+N A+FRRV LEKY K+FFARKLEGKSYLE MR+
Sbjct: 301 ERLSVATFHSSNLNSELGPANSLVGPHNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRI 360

Query: 361 KNVGDQDHVC 371
           +N+G  DH C
Sbjct: 361 ENMG--DHAC 363

BLAST of MS010974 vs. ExPASy Swiss-Prot
Match: D4N501 (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum OX=3469 GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 6.4e-106
Identity = 190/356 (53.37%), Postives = 261/356 (73.31%), Query Frame = 0

Query: 11  GRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDG--ESEPSVPVVDIHRLVLGDS 70
           G  + +PSVQELAK  + +IP RY  T ++ L +PV     +   +VPV+DI  L+  + 
Sbjct: 10  GNGMSIPSVQELAKLTLAEIPSRYICTVEN-LQLPVGASVIDDHETVPVIDIENLISSEP 69

Query: 71  ASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQ 130
            +   EL +LHSAC EWGFFQVVNHGV TSL++  + +++ FFNL  +EK    Q   + 
Sbjct: 70  VTEKLELDRLHSACKEWGFFQVVNHGVDTSLVDNVKSDIQGFFNLSMNEKIKYGQKDGDV 129

Query: 131 EGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAML 190
           EGFGQ FV SE+Q LDW+D+F + TLPL+LR PH+F KLP  LRET+E+YS+E+KKL+M+
Sbjct: 130 EGFGQAFVASEDQTLDWADIFMILTLPLHLRKPHLFSKLPLPLRETIESYSSEMKKLSMV 189

Query: 191 ILGRLAVALKMDGEEMREL---FRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTILY 250
           +  ++  AL++   E++E+   F+D  Q MRMNYYPPCPQP+ AIG +PHSD   LTIL 
Sbjct: 190 LFEKMEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILL 249

Query: 251 QLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSV 310
           QLNE EGLQ++N GRWI+VKPLPNAFVVN+GD++EI++NG+Y+S++HR   N +KERLS+
Sbjct: 250 QLNEVEGLQIKNEGRWISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSI 309

Query: 311 ATFYSSNLDSELGPANSLIGPNNRAIFRR-VPLEKYLKEFFARKLEGKSYLEYMRM 361
           ATF+  NL+SE+GP +SLI PN  A+FR      + ++EF +RKL+GKS+L+ MRM
Sbjct: 310 ATFHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

BLAST of MS010974 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-102
Identity = 178/351 (50.71%), Postives = 255/351 (72.65%), Query Frame = 0

Query: 13  SVIVPSVQELAKH-PILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRLVLGDSASA 72
           S++VPSVQE+ K   I  +P RY R+DQD   V   D + +  +P++D+ RL    S + 
Sbjct: 11  SILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVD-DDFDVKIEIPIIDMKRLC--SSTTM 70

Query: 73  AAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQEGF 132
            +E++KL  AC EWGFFQ+VNHG+ +S L++ + E++ FFNLP  EKK  WQ P+  EGF
Sbjct: 71  DSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGF 130

Query: 133 GQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLILG 192
           GQ FVVSE+QKLDW+D+FF T  P+ LR PH+F KLP   R+TLE YS+EV+ +A +++ 
Sbjct: 131 GQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIA 190

Query: 193 RLAVALKMDGEEMRELFR--DGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTILYQLNE 252
           ++A AL++  EE+ +LF   D  Q+MRMNYYPPCPQPD+ IG +PHSD+  LT+L Q+N+
Sbjct: 191 KMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVND 250

Query: 253 TEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSVATFY 312
            EGLQ++ +G+W+ VKPLPNAF+VNIGD++EI++NG Y+SIEHR   N  KERLS+ATF+
Sbjct: 251 VEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFH 310

Query: 313 SSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
           +  +  E+GPA SL+     A F+R+ +++Y    F+R L+GK+YL+ +R+
Sbjct: 311 NVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of MS010974 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 5.6e-102
Identity = 178/351 (50.71%), Postives = 250/351 (71.23%), Query Frame = 0

Query: 11  GRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRLVLGDSAS 70
           G  + +PSVQELAK  + +IP RY  T + PL         + +VPV+D+  L+  +   
Sbjct: 10  GNGLSIPSVQELAKLTLAEIPSRYTCTGESPLNNIGASVTDDETVPVIDLQNLLSPEPVV 69

Query: 71  AAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQEG 130
              EL KLHSAC EWGFFQ+VNHGV   L++  + E++ FFNLP +EK    Q   + EG
Sbjct: 70  GKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLPMNEKTKYGQQDGDFEG 129

Query: 131 FGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLIL 190
           FGQ ++ SE+Q+LDW+++F M +LPL+LR PH+F +LP   RETLE+Y +++KKL+ ++ 
Sbjct: 130 FGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRETLESYLSKMKKLSTVVF 189

Query: 191 GRLAVALKM-DGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTILYQLNE 250
             L  +L++ + + M +LF DG QTMRMNYYPPCP+P+  +G + HSD   LTIL QLNE
Sbjct: 190 EMLEKSLQLVEIKGMTDLFEDGLQTMRMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNE 249

Query: 251 TEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSVATFY 310
            EGLQ+R   RWI++KPLP+AF+VN+GDI+EI++NG+Y+S+EHR   N +KERLS+ATF+
Sbjct: 250 VEGLQIRKEERWISIKPLPDAFIVNVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFH 309

Query: 311 SSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
            S L+SE+GP +SL+ P   A+F+R   E  LKE  +RKL+GKS+L+YMRM
Sbjct: 310 DSKLESEIGPISSLVTPETPALFKRGRYEDILKENLSRKLDGKSFLDYMRM 360

BLAST of MS010974 vs. ExPASy Swiss-Prot
Match: D4N500 (Thebaine 6-O-demethylase OS=Papaver somniferum OX=3469 GN=T6ODM PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 7.1e-97
Identity = 180/358 (50.28%), Postives = 246/358 (68.72%), Query Frame = 0

Query: 11  GRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGES----EPSVPVVDIHRLVLG 70
           G  + +PSVQELAK  + +IP RY   +++ L+   P G S      ++PV+DI  L+  
Sbjct: 10  GNGMEIPSVQELAKLTLAEIPSRYVCANENLLL---PMGASVINDHETIPVIDIENLLSP 69

Query: 71  DSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPE 130
           +      EL +LH AC EWGFFQVVNHGV  SL++  + E++ FFNL   EK    Q   
Sbjct: 70  EPIIGKLELDRLHFACKEWGFFQVVNHGVDASLVDSVKSEIQGFFNLSMDEKTKYEQEDG 129

Query: 131 NQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLA 190
           + EGFGQ F+ SE+Q LDW+D+F M TLPL+LR PH+F KLP  LRET+E+YS+E+KKL+
Sbjct: 130 DVEGFGQGFIESEDQTLDWADIFMMFTLPLHLRKPHLFSKLPVPLRETIESYSSEMKKLS 189

Query: 191 MLILGRLAVALKMDGEE---MRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTI 250
           M++  ++  AL++   E   M E+F DG Q MRMNYYPPCPQP+ AIG + HSD   LTI
Sbjct: 190 MVLFNKMEKALQVQAAEIKGMSEVFIDGTQAMRMNYYPPCPQPNLAIGLTSHSDFGGLTI 249

Query: 251 LYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERL 310
           L Q+NE EGLQ++  G WI+VKPLPNAFVVN+GDI+EI++NG+Y S++HR   N + ERL
Sbjct: 250 LLQINEVEGLQIKREGTWISVKPLPNAFVVNVGDILEIMTNGIYHSVDHRAVVNSTNERL 309

Query: 311 SVATFYSSNLDSELGPANSLIGPNNRAIFRR-VPLEKYLKEFFARKLEGKSYLEYMRM 361
           S+ATF+  +L+S +GP +SLI P   A+F+        ++E   RKL+GKS+L+ MR+
Sbjct: 310 SIATFHDPSLESVIGPISSLITPETPALFKSGSTYGDLVEECKTRKLDGKSFLDSMRI 364

BLAST of MS010974 vs. ExPASy Swiss-Prot
Match: A2A1A0 (S-norcoclaurine synthase 1 OS=Coptis japonica OX=3442 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 2.1e-72
Identity = 145/355 (40.85%), Postives = 214/355 (60.28%), Query Frame = 0

Query: 11  GRSVIVPSVQELAKHPILKIPLRYQRTD-QDPLIVPVPDGESEPSVPVVDIHRLVLGDSA 70
           G S+ V +VQ LA   +  +P RY R + +   +VP+   ++   +PV+D+ RL+  D  
Sbjct: 9   GGSLPVENVQVLAGKELKNLPNRYVRPELEHDDVVPI---DNSLEIPVIDLSRLL--DQQ 68

Query: 71  SAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQE 130
            A  EL K HSAC +WGFFQ++NHGV   ++E+ +++ E FF LP+ EK    Q P   E
Sbjct: 69  YACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPFKEKNAYRQLPNGME 128

Query: 131 GFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLI 190
           G+GQ FV SEEQKLDW+DM F+ T P+  RN   +   P   RET+E YS E++K+AM +
Sbjct: 129 GYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETMEKYSMELQKVAMCL 188

Query: 191 LGRLAVALKMDGE----EMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTILY 250
            G +A  L ++ E     +R +F    + +     P      + +G SPHSDA  LT+L 
Sbjct: 189 TGMMAKNLGLESEILTKPLRTVFNREDELL-----PSMSSCGEGLGLSPHSDATGLTLLI 248

Query: 251 QLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSV 310
           Q+NE  GL ++ + +W+ +KP+  AFVVNIGD++EI+SNG+YKSIEHR   N  KERLS+
Sbjct: 249 QVNEVNGLHIKKDEKWVPIKPILGAFVVNIGDVIEIMSNGIYKSIEHRAVINTDKERLSI 308

Query: 311 ATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
           A F+     +++GP   L+   N   ++ +  E YL      KL+GKS L+ M++
Sbjct: 309 AAFHDPEYGTKIGPLPDLV-KENGVKYKTIDYEDYLIRSSNIKLDGKSLLDQMKL 352

BLAST of MS010974 vs. ExPASy TrEMBL
Match: A0A6J1CLK9 (probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Momordica charantia OX=3673 GN=LOC111012286 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.2e-213
Identity = 368/370 (99.46%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTD+DPLIVPVPDGESEPSVPVVDI
Sbjct: 3   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDRDPLIVPVPDGESEPSVPVVDI 62

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL
Sbjct: 63  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 122

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST
Sbjct: 123 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 182

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA
Sbjct: 183 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 242

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNET+GLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK
Sbjct: 243 LTILYQLNETKGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 302

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM
Sbjct: 303 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 362

Query: 361 KNVGDQDHVC 371
           KNVGDQDHVC
Sbjct: 363 KNVGDQDHVC 372

BLAST of MS010974 vs. ExPASy TrEMBL
Match: A0A6J1ILW1 (protein SRG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478593 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.4e-164
Identity = 290/370 (78.38%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   NFG S+IVPSV ELAK P+ KIPLRY+R DQDP +  V D +S PSVP+VD+
Sbjct: 1   MESNTEIVNFGTSIIVPSVLELAKQPLAKIPLRYERADQDPPM--VSDRDSGPSVPIVDL 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRL +GD  SAA+EL KLHSAC EWGFFQ++NH VPTSLLEEFR+E+ESFFNLPY EKKL
Sbjct: 61  HRLAVGD--SAASELDKLHSACKEWGFFQIINHEVPTSLLEEFRMEIESFFNLPYAEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN +NQEGFGQLFVVSEEQKLDWSDMF++TTLPL+LR P +FQKLPPKLRETLEAYST
Sbjct: 121 LWQNSQNQEGFGQLFVVSEEQKLDWSDMFYITTLPLDLREPRLFQKLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLA++IL  LA ALKMD EEMRELFRDG Q+MR+NYYPPCP+PDKAIGFS HSDADA
Sbjct: 181 EVKKLAIVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRW+AVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVS N SK
Sbjct: 241 LTILYQLNEAEGLQIRKDGRWVAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSLNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATF+SSNL+S+LGPANSL+GP N A+FRRV LEKY K+FFARKLEGKSYLE MR+
Sbjct: 301 ERLSVATFHSSNLNSDLGPANSLVGPQNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRI 360

Query: 361 KNVGDQDHVC 371
           +N+G  DH C
Sbjct: 361 ENMG--DHAC 364

BLAST of MS010974 vs. ExPASy TrEMBL
Match: A0A6J1EG75 (protein SRG1-like OS=Cucurbita moschata OX=3662 GN=LOC111433084 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.2e-163
Identity = 290/370 (78.38%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   NFG S+IVPSV ELAK P+ KIPLRY+R DQDP +  V D +S PSVPVVD+
Sbjct: 1   MESNTEIVNFGTSIIVPSVLELAKQPLSKIPLRYERADQDPPM--VSDRDSGPSVPVVDL 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRL +GDSA AA+EL KLHSAC EWGFFQ++NH V TSLLEEFR+E+ESFFNLPY EKKL
Sbjct: 61  HRLAVGDSA-AASELDKLHSACKEWGFFQIINHEVSTSLLEEFRMEIESFFNLPYAEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN +NQEGFGQLFVVSEEQKLDWSDMF++TTLPL+LR P +FQKLPPKLRETLEAYST
Sbjct: 121 LWQNSQNQEGFGQLFVVSEEQKLDWSDMFYITTLPLDLREPRLFQKLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLA++IL  LA ALKMD EEMRELFRDG Q+MR+NYYPPCP+PDKAIGFS HSDADA
Sbjct: 181 EVKKLAIVILDHLAKALKMDVEEMRELFRDGVQSMRLNYYPPCPEPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRW++VKPLPNAFV NIGDIMEIVSNGVYKSIEHRVS N SK
Sbjct: 241 LTILYQLNEAEGLQIRKDGRWVSVKPLPNAFVANIGDIMEIVSNGVYKSIEHRVSLNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATF+SSNL+SELGPANSL+GP+N A+FRRV LEKY K+FFARKLEGKSYLE MR+
Sbjct: 301 ERLSVATFHSSNLNSELGPANSLVGPHNPAVFRRVMLEKYFKDFFARKLEGKSYLEQMRI 360

Query: 361 KNVGDQDHVC 371
           +N+G  DH C
Sbjct: 361 ENMG--DHAC 365

BLAST of MS010974 vs. ExPASy TrEMBL
Match: A0A0A0KLU1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G520990 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 3.0e-162
Identity = 291/370 (78.65%), Postives = 322/370 (87.03%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   +FG S++VPSV ELAK PI KI LRY+R DQDP I  VP GES PSVPVVDI
Sbjct: 1   MESNTQMVDFGTSIVVPSVIELAKRPIPKISLRYERLDQDPPI--VPGGESGPSVPVVDI 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           HRL +G SAS   E+  LHSAC EWGFFQ++NHGV T+LLEEFR+EVESFFNLPY EKKL
Sbjct: 61  HRLAIGGSAS--PEIDTLHSACKEWGFFQIINHGVSTTLLEEFRMEVESFFNLPYDEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQN ENQEGFGQLFVVSEEQKLDWSDMF++TTLPLNLR PH+FQ+LPPKLRETLEAYST
Sbjct: 121 LWQNSENQEGFGQLFVVSEEQKLDWSDMFYITTLPLNLRKPHLFQRLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLAM+ILG LA ALKMD EEMRELF DG Q++RMNYYPPCP PDKAIGFS HSDADA
Sbjct: 181 EVKKLAMVILGHLAEALKMDVEEMRELFGDGVQSVRMNYYPPCPVPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRW++VKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSN SK
Sbjct: 241 LTILYQLNEVEGLQIRKDGRWVSVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATFYSSN++SELGPA SLIGP+N A+FRRV LEKY ++FFARKLE KSYLE+MR+
Sbjct: 301 ERLSVATFYSSNINSELGPAKSLIGPHNPAVFRRVLLEKYFRDFFARKLERKSYLEHMRI 360

Query: 361 KNVGDQDHVC 371
           +  G  DH C
Sbjct: 361 ETEG--DHSC 364

BLAST of MS010974 vs. ExPASy TrEMBL
Match: A0A5D3CMW3 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008600 PE=3 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 2.0e-158
Identity = 287/370 (77.57%), Postives = 319/370 (86.22%), Query Frame = 0

Query: 1   LNSETGTGNFGRSVIVPSVQELAKHPILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDI 60
           + S T   +FG S+IVPSV EL K  I KIPLRY+R DQDP I   P  ES PSVPVVDI
Sbjct: 1   MESNTQMVDFGTSIIVPSVLELTKRAIPKIPLRYERLDQDPPI--DPGRESGPSVPVVDI 60

Query: 61  HRLVLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKL 120
           H L +G SAS   E+ KLHSAC EWGFFQ++NHGV T+LLEEFR+EVESFFNLPY EKKL
Sbjct: 61  HGLAVGGSAS--PEIDKLHSACKEWGFFQIINHGVSTTLLEEFRMEVESFFNLPYDEKKL 120

Query: 121 LWQNPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYST 180
           LWQ+ +NQEGFGQLFVVSEEQKLDWSDMF++TTLPLNLR PH+FQ+LPPKLRETLEAYST
Sbjct: 121 LWQDSKNQEGFGQLFVVSEEQKLDWSDMFYITTLPLNLRKPHLFQRLPPKLRETLEAYST 180

Query: 181 EVKKLAMLILGRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADA 240
           EVKKLAM+IL  LA ALKMD EEMRELF DG Q++RMNYYPPCP PDKAIGFS HSDADA
Sbjct: 181 EVKKLAMVILDHLAGALKMDVEEMRELFGDGVQSVRMNYYPPCPVPDKAIGFSAHSDADA 240

Query: 241 LTILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSK 300
           LTILYQLNE EGLQ+R +GRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSN SK
Sbjct: 241 LTILYQLNEVEGLQIRKDGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNFSK 300

Query: 301 ERLSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 360
           ERLSVATFYS NL+SE+GPA SLIGP+N A+F+RV LEKY ++FFARKLEGKSYLE+MR+
Sbjct: 301 ERLSVATFYSLNLNSEVGPAKSLIGPHNPAVFQRVMLEKYFRDFFARKLEGKSYLEHMRI 360

Query: 361 KNVGDQDHVC 371
           ++ G  DH C
Sbjct: 361 ESEG--DHSC 364

BLAST of MS010974 vs. TAIR 10
Match: AT4G25300.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 387.1 bits (993), Expect = 1.6e-107
Identity = 190/350 (54.29%), Postives = 251/350 (71.71%), Query Frame = 0

Query: 13  SVIVPSVQELAKHP-ILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRLVLGDSASA 72
           S+IVPSVQE+ K   I  +P RY R+DQD   + V  G     +P++D+   +L  S S 
Sbjct: 10  SIIVPSVQEMVKEKMITTVPPRYVRSDQDVAEIAVDSG-LRNQIPIIDMS--LLCSSTSM 69

Query: 73  AAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQEGF 132
            +E+ KL SAC EWGFFQ+VNHG+ +S L + + EV+ FFNLP  EKK LWQ P+  EGF
Sbjct: 70  DSEIDKLDSACKEWGFFQLVNHGMESSFLNKVKSEVQDFFNLPMEEKKNLWQQPDEIEGF 129

Query: 133 GQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLILG 192
           GQ+FVVSEEQKLDW+DMFF+T  P+ LR PH+F KLP   R+TL+ YS EVK +A ++LG
Sbjct: 130 GQVFVVSEEQKLDWADMFFLTMQPVRLRKPHLFPKLPLPFRDTLDMYSAEVKSIAKILLG 189

Query: 193 RLAVALKMDGEEMRELFRDG-HQTMRMNYYPPCPQPDKAIGFSPHSDADALTILYQLNET 252
           ++AVALK+  EEM +LF D   Q +R+NYYP CP+PDK IG +PHSD+  LTIL Q NE 
Sbjct: 190 KIAVALKIKPEEMDKLFDDELGQRIRLNYYPRCPEPDKVIGLTPHSDSTGLTILLQANEV 249

Query: 253 EGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSVATFYS 312
           EGLQ++ N +W++VKPLPNA VVN+GDI+EI++NG Y+SIEHR   N  KERLSVA F++
Sbjct: 250 EGLQIKKNAKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKERLSVAAFHN 309

Query: 313 SNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
             L  E+GP  SL+  +  A F+ V  E+Y    F+R+L+GK+YL+ MR+
Sbjct: 310 IGLGKEIGPMRSLVERHKAAFFKSVTTEEYFNGLFSRELDGKAYLDVMRL 356

BLAST of MS010974 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.2e-104
Identity = 185/358 (51.68%), Postives = 256/358 (71.51%), Query Frame = 0

Query: 6   GTGNFGRSVIVPSVQELAKHPILK--IPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRL 65
           G G    SVIVPSVQE+ K  ++   +P RY R+DQ+     +  GE++  +P++D+   
Sbjct: 3   GKGVTFSSVIVPSVQEMVKEKVITTVLPPRYVRSDQEKGEAAIDSGENQ--IPIIDMS-- 62

Query: 66  VLGDSASAAAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQ 125
           +L  S S  +E+ KL  AC EWGFFQ+VNHG+    L++F+ +++ FFNLP  EKK LWQ
Sbjct: 63  LLSSSTSMDSEIDKLDFACKEWGFFQLVNHGMD---LDKFKSDIQDFFNLPMEEKKKLWQ 122

Query: 126 NPENQEGFGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVK 185
            P + EGFGQ FV SEEQKLDW+D+FF+T  P+ LR PH+F KLP   R+TL+ YS E+K
Sbjct: 123 QPGDIEGFGQAFVFSEEQKLDWADVFFLTMQPVPLRKPHLFPKLPLPFRDTLDTYSAELK 182

Query: 186 KLAMLILGRLAVALKMDGEEMRELFRDG-HQTMRMNYYPPCPQPDKAIGFSPHSDADALT 245
            +A ++  +LA ALK+  EEM +LF D   Q +RMNYYPPCP+PDKAIG +PHSDA  LT
Sbjct: 183 SIAKVLFAKLASALKIKPEEMEKLFDDELGQRIRMNYYPPCPEPDKAIGLTPHSDATGLT 242

Query: 246 ILYQLNETEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKER 305
           IL Q+NE EGLQ++ +G+W++VKPLPNA VVN+GDI+EI++NG Y+SIEHR   N  KER
Sbjct: 243 ILLQVNEVEGLQIKKDGKWVSVKPLPNALVVNVGDILEIITNGTYRSIEHRGVVNSEKER 302

Query: 306 LSVATFYSSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
           LSVA+F+++    E+GP  SL+  +  A+F+ +  E+Y    F+R+L+GK+YL+ MR+
Sbjct: 303 LSVASFHNTGFGKEIGPMRSLVERHKGALFKTLTTEEYFHGLFSRELDGKAYLDVMRI 353

BLAST of MS010974 vs. TAIR 10
Match: AT1G17020.1 (senescence-related gene 1 )

HSP 1 Score: 374.8 bits (961), Expect = 8.1e-104
Identity = 178/351 (50.71%), Postives = 255/351 (72.65%), Query Frame = 0

Query: 13  SVIVPSVQELAKH-PILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRLVLGDSASA 72
           S++VPSVQE+ K   I  +P RY R+DQD   V   D + +  +P++D+ RL    S + 
Sbjct: 11  SILVPSVQEMVKEKTITTVPPRYVRSDQDKTEVD-DDFDVKIEIPIIDMKRLC--SSTTM 70

Query: 73  AAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQEGF 132
            +E++KL  AC EWGFFQ+VNHG+ +S L++ + E++ FFNLP  EKK  WQ P+  EGF
Sbjct: 71  DSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGF 130

Query: 133 GQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLILG 192
           GQ FVVSE+QKLDW+D+FF T  P+ LR PH+F KLP   R+TLE YS+EV+ +A +++ 
Sbjct: 131 GQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIA 190

Query: 193 RLAVALKMDGEEMRELFR--DGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTILYQLNE 252
           ++A AL++  EE+ +LF   D  Q+MRMNYYPPCPQPD+ IG +PHSD+  LT+L Q+N+
Sbjct: 191 KMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVND 250

Query: 253 TEGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSVATFY 312
            EGLQ++ +G+W+ VKPLPNAF+VNIGD++EI++NG Y+SIEHR   N  KERLS+ATF+
Sbjct: 251 VEGLQIKKDGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFH 310

Query: 313 SSNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
           +  +  E+GPA SL+     A F+R+ +++Y    F+R L+GK+YL+ +R+
Sbjct: 311 NVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYLDALRI 358

BLAST of MS010974 vs. TAIR 10
Match: AT1G17010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 374.0 bits (959), Expect = 1.4e-103
Identity = 180/351 (51.28%), Postives = 248/351 (70.66%), Query Frame = 0

Query: 13  SVIVPSVQELAKHP-ILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRLVLGDSASA 72
           S++VPSVQE+ K   I  +P RY R DQD   V V D      +P++D++RL    S + 
Sbjct: 11  SILVPSVQEMVKDKMITTVPPRYVRYDQDKTEVVVHDSGLISEIPIIDMNRLC--SSTAV 70

Query: 73  AAELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQEGF 132
            +E++KL  AC E+GFFQ+VNHG+  S L++ + E++ FFNLP  EKK LWQ P   EGF
Sbjct: 71  DSEVEKLDFACKEYGFFQLVNHGIDPSFLDKIKSEIQDFFNLPMEEKKKLWQTPAVMEGF 130

Query: 133 GQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLILG 192
           GQ FVVSE+QKLDW+D+FF+   P+ LR  H+F KLP   R+TL+ YST VK +A ++L 
Sbjct: 131 GQAFVVSEDQKLDWADLFFLIMQPVQLRKRHLFPKLPLPFRDTLDMYSTRVKSIAKILLA 190

Query: 193 RLAVALKMDGEEMRELFRDG-HQTMRMNYYPPCPQPDKAIGFSPHSDADALTILYQLNET 252
           ++A AL++  EE+ E+F D   Q+MRMNYYPPCPQP+   G  PHSDA  LTIL Q+NE 
Sbjct: 191 KMAKALQIKPEEVEEIFGDDMMQSMRMNYYPPCPQPNLVTGLIPHSDAVGLTILLQVNEV 250

Query: 253 EGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSVATFYS 312
           +GLQ++ NG+W  VKPL NAF+VN+GD++EI++NG Y+SIEHR   N  KERLS+ATF++
Sbjct: 251 DGLQIKKNGKWFFVKPLQNAFIVNVGDVLEIITNGTYRSIEHRAMVNLEKERLSIATFHN 310

Query: 313 SNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRMK 362
           + +D E+GPA SL+     A FR +  + YL   F+R+L+GK+YL+ MR++
Sbjct: 311 TGMDKEIGPARSLVQRQEAAKFRSLKTKDYLNGLFSRELKGKAYLDAMRIE 359

BLAST of MS010974 vs. TAIR 10
Match: AT1G78550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.6e-96
Identity = 172/350 (49.14%), Postives = 239/350 (68.29%), Query Frame = 0

Query: 13  SVIVPSVQELAKHP-ILKIPLRYQRTDQDPLIVPVPDGESEPSVPVVDIHRLVLGDSASA 72
           S+IVP V E+ K      IP RY R DQ+   + + D      +PV+D+ RL    S SA
Sbjct: 11  SLIVPFVLEIVKEKNFTTIPPRYVRVDQEKTEI-LNDSSLSSEIPVIDMTRLC---SVSA 70

Query: 73  A-AELQKLHSACSEWGFFQVVNHGVPTSLLEEFRIEVESFFNLPYHEKKLLWQNPENQEG 132
             +EL+KL  AC +WGFFQ+VNHG+ +S LE+   EV+ FFNLP  EK+ LWQ     EG
Sbjct: 71  MDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLPMKEKQKLWQRSGEFEG 130

Query: 133 FGQLFVVSEEQKLDWSDMFFMTTLPLNLRNPHIFQKLPPKLRETLEAYSTEVKKLAMLIL 192
           FGQ+ +VSE QKLDW DMF +TT P+  R  H+F KLPP  RETLE YS+EVK +A ++ 
Sbjct: 131 FGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRETLETYSSEVKSIAKILF 190

Query: 193 GRLAVALKMDGEEMRELFRDGHQTMRMNYYPPCPQPDKAIGFSPHSDADALTILYQLNET 252
            ++A  L++  EEM +LF D  Q++++NYYPPCPQPD+ +G + HSDA  LTIL Q+N+ 
Sbjct: 191 AKMASVLEIKHEEMEDLFDDVWQSIKINYYPPCPQPDQVMGLTQHSDAAGLTILLQVNQV 250

Query: 253 EGLQVRNNGRWIAVKPLPNAFVVNIGDIMEIVSNGVYKSIEHRVSSNCSKERLSVATFYS 312
           EGLQ++ +G+W+ VKPL +A VVN+G+I+EI++NG Y+SIEHR   N  KERLSVA F+S
Sbjct: 251 EGLQIKKDGKWVVVKPLRDALVVNVGEILEIITNGRYRSIEHRAVVNSEKERLSVAMFHS 310

Query: 313 SNLDSELGPANSLIGPNNRAIFRRVPLEKYLKEFFARKLEGKSYLEYMRM 361
              ++ + PA SL+    + +F+ +  ++Y   FF +KL GKS+L+ MR+
Sbjct: 311 PGKETIIRPAKSLVDRQKQCLFKSMSTQEYFDAFFTQKLNGKSHLDLMRI 356