Homology
BLAST of MS010969 vs. NCBI nr
Match:
XP_022142085.1 (subtilisin-like protease SBT5.6 [Momordica charantia])
HSP 1 Score: 1558.9 bits (4035), Expect = 0.0e+00
Identity = 780/785 (99.36%), Postives = 780/785 (99.36%), Query Frame = 0
Query: 1 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 60
MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV
Sbjct: 32 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 91
Query: 61 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFS 120
KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVV VIRSEKYSTQTTRSWEFS
Sbjct: 92 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVRVIRSEKYSTQTTRSWEFS 151
Query: 121 GVEEDKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAF 180
GVEEDKPRLNDLVSRAKFGKDVVI MLDSGVWPKSKSFSDKGMGPIP SWRGICQTGPAF
Sbjct: 152 GVEEDKPRLNDLVSRAKFGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWRGICQTGPAF 211
Query: 181 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 240
QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF
Sbjct: 212 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 271
Query: 241 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 300
GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS
Sbjct: 272 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 331
Query: 301 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 360
IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE
Sbjct: 332 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 391
Query: 361 FYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 420
FYSPVILGNGFKIRGLSVAPS LKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA
Sbjct: 392 FYSPVILGNGFKIRGLSVAPSMLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 451
Query: 421 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 480
RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN
Sbjct: 452 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 511
Query: 481 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 540
IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA
Sbjct: 512 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 571
Query: 541 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 600
AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT
Sbjct: 572 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 631
Query: 601 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 660
TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK
Sbjct: 632 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 691
Query: 661 MAHSIDPSFTCPARARAHDLNYPSVAVPRLR-GGGGGVRVVRTVRNVGGSKSAYFFRASA 720
MAHSIDPSFTCPARARAHDLNYPSVAVPRLR GGGGGVRVVRTVRNVGGSKSAYFFRASA
Sbjct: 692 MAHSIDPSFTCPARARAHDLNYPSVAVPRLRGGGGGGVRVVRTVRNVGGSKSAYFFRASA 751
Query: 721 PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI 780
PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI
Sbjct: 752 PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI 811
Query: 781 AISSA 785
AISSA
Sbjct: 812 AISSA 816
BLAST of MS010969 vs. NCBI nr
Match:
XP_038882517.1 (subtilisin-like protease SBT5.6 [Benincasa hispida])
HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 648/787 (82.34%), Postives = 710/787 (90.22%), Query Frame = 0
Query: 1 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 60
MEK S F +LLLLL + QAS S N+ AYIVYFGEH GDK+W EIEA HHSYL SV
Sbjct: 1 MEKSSFFCFVLLLLLPFIQLQASSSHNH--KAYIVYFGEHHGDKSWEEIEARHHSYLMSV 60
Query: 61 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFS 120
K++EE+A+SSL+Y+YKHSIN FAA+L+ ++ASKLSE+D VVSVI S+KY TTRSWEFS
Sbjct: 61 KESEEDAKSSLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRMHTTRSWEFS 120
Query: 121 GVEEDKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAF 180
GVEEDKP +NDLV RA +GKDVVI MLDSGVWPKSKSFSDKGMGPIP SW+GICQTGPAF
Sbjct: 121 GVEEDKPTINDLVYRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGICQTGPAF 180
Query: 181 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 240
QSAHCNRKIIGARYYLKGYE++FGRLNET DYRSPCDKDGHGSHTAS AGGRRV+NVSAF
Sbjct: 181 QSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGRRVYNVSAF 240
Query: 241 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 300
GGVA GTASGGAP ARLAIYKVCWAIP QMK LGNVCFDTD+LAAMDDAIADGVDVLSLS
Sbjct: 241 GGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADGVDVLSLS 300
Query: 301 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 360
IGK+DPYNYTDDGIAIGALHAVK+DIVVSCSAGNYGPTPSALSN APWIITVGASTVDRE
Sbjct: 301 IGKSDPYNYTDDGIAIGALHAVKRDIVVSCSAGNYGPTPSALSNIAPWIITVGASTVDRE 360
Query: 361 FYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 420
FYSPVILGNG KI+GLSVAPSKLKRKKMYPLVYAGDI+KPHVP+NQSG+CVAGSLSHEKA
Sbjct: 361 FYSPVILGNGLKIKGLSVAPSKLKRKKMYPLVYAGDILKPHVPRNQSGLCVAGSLSHEKA 420
Query: 421 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 480
+GKIVLC+R EGISR+AGSLEVKRSGGAGMILGNVPAVGR+ HADPHFVPATAVSYEDAN
Sbjct: 421 KGKIVLCFRGEGISRFAGSLEVKRSGGAGMILGNVPAVGRRPHADPHFVPATAVSYEDAN 480
Query: 481 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 540
+ILKYIKSRTNPTA IVPPITIYGSRPAPAMANFSSRGPNP+DPH LKPDITAPGVDILA
Sbjct: 481 MILKYIKSRTNPTANIVPPITIYGSRPAPAMANFSSRGPNPVDPHFLKPDITAPGVDILA 540
Query: 541 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 600
AWS +DSPTKLPK LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHP+WSQAAIRSALMT
Sbjct: 541 AWSEQDSPTKLPKYLDPRIVKYNLYSGTSMSCPHVSAAAALLRAIHPSWSQAAIRSALMT 600
Query: 601 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 660
TATT NK G P+TDDAS ++PA+PFS+GSGHFRPSKAADPGLVYD+NYTDYL +LC LK
Sbjct: 601 TATTINKSGHPLTDDASPDNSPASPFSFGSGHFRPSKAADPGLVYDSNYTDYLHYLCGLK 660
Query: 661 MAHSIDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKSAYFFRAS 720
M +SIDPSFTCP RA HDLNYPS+AVPRLR G VR+ RTV NV GG KS YFF +
Sbjct: 661 M-NSIDPSFTCPPRALHPHDLNYPSIAVPRLR---GAVRIKRTVTNVGGGGKSVYFFHSE 720
Query: 721 APAGVSVRASPSVLYFSRMGQRKRFTITISGKAE--IGGVDGSGYSFGWFAWTDGIHFVR 780
AP GV+V ASP++LYF+R+G+RKRFTIT+SGK + + +GYSFGWFAWTDGIH+VR
Sbjct: 721 APPGVAVSASPNILYFNRVGERKRFTITVSGKVKNSNNNDNNNGYSFGWFAWTDGIHYVR 780
Query: 781 SPIAISS 784
SPIA+SS
Sbjct: 781 SPIAVSS 781
BLAST of MS010969 vs. NCBI nr
Match:
XP_004134922.1 (subtilisin-like protease SBT5.6 isoform X2 [Cucumis sativus] >KGN49345.1 hypothetical protein Csa_003109 [Cucumis sativus])
HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 639/795 (80.38%), Postives = 710/795 (89.31%), Query Frame = 0
Query: 1 MEKYSTFVC---LLLLLLLLL----FQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATH 60
MEK S+F+C LLLLLLLLL QQA+ S NN + AYIVYFGEH G+K+ EI+ H
Sbjct: 1 MEK-SSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERH 60
Query: 61 HSYLTSVKDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQT 120
HSYL VK++EE+A+S L+Y+YKHSIN FAA+L+ ++ASKLS++D VVSVI S+KY +T
Sbjct: 61 HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMET 120
Query: 121 TRSWEFSGVEEDKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGI 180
TRSWEFSGVEEDKP +NDLVSRA +GKDVVI MLDSGVWPKSKSFSDKGMGPIP SW+GI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGI 180
Query: 181 CQTGPAFQSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRR 240
CQTGPAFQSAHCNRKIIGARYYLKGYE++FGRLN+T DYRSPCDKDGHGSHTAS AGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRR 240
Query: 241 VFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADG 300
V+NVSAFGGVA GTASGGAP ARLAIYKVCWAIP QMK LGNVCFDTD+LAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300
Query: 301 VDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVG 360
VDVLSLSIGK++PYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSN APWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360
Query: 361 ASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAG 420
ASTVDREFYSPVILGNG KI+GLSVAPSKL+RKKMYPLVYAGDIM PH P+NQSG+CVAG
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420
Query: 421 SLSHEKARGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATA 480
SLSHEKA+GKIVLC+R EGISR+AGSLEV+RSGGAGMILGNVPAVGR+ HADPHFVPATA
Sbjct: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
Query: 481 VSYEDANIILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITA 540
VSYEDANIILKYIKSR NPTATIVPP+TIYGSRPAPAMANFSSRGPNPIDPH LKPDITA
Sbjct: 481 VSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540
Query: 541 PGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAA 600
PGVDILAAWS +DSPTKLPK LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHPTWSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600
Query: 601 IRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYL 660
IRSALMTT+TT NKYG+PITDD++ ++PATPFS+GSGHFRPSKAADPGLVYD+NYTDYL
Sbjct: 601 IRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660
Query: 661 VHLCALKMAHSIDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKS 720
+LC LKM +SIDPSF CP RA HDLNYPS+AVP+LR VR+ RTV NV GG K+
Sbjct: 661 HYLCGLKM-NSIDPSFKCPPRALHPHDLNYPSIAVPQLR---NVVRIKRTVTNVGGGGKN 720
Query: 721 AYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTITISGKA---EIGGVDGSGYSFGWFAW 780
YFF++ AP GV+V ASP++LYF+R+G+RK+FTITIS K G YSFGWFAW
Sbjct: 721 VYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAW 780
Query: 781 TDGIHFVRSPIAISS 784
+DGIH+VRSPIA+SS
Sbjct: 781 SDGIHYVRSPIAVSS 790
BLAST of MS010969 vs. NCBI nr
Match:
XP_022926980.1 (subtilisin-like protease SBT5.6 [Cucurbita moschata])
HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 632/781 (80.92%), Postives = 697/781 (89.24%), Query Frame = 0
Query: 5 STFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTE 64
S+ CLLLLLLLL S + AYIVYFGEH GDK+W EIE HHSYL SVKDTE
Sbjct: 4 SSLFCLLLLLLLL----PPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 63
Query: 65 EEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEE 124
+A+SSL+YSYKH+INGFAAVL+ ++ASKLSE++ VVSVI S+KY TTRSWEFSGVEE
Sbjct: 64 NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGVEE 123
Query: 125 DKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 184
DKPRL+DLVS+AK GKDVVI MLDSGVWPKSKSFSDKGMGP+P SW+GICQTGPAF+S+H
Sbjct: 124 DKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFESSH 183
Query: 185 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 244
CNRKIIGARYY+KGYE++FGRLNET D+RSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 184 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 243
Query: 245 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 304
RGTASGGAPG RLAIYKVCWAIP QMK LGNVCFDTDVLAAMDDAI+DGVDVLSLSIGK+
Sbjct: 244 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 303
Query: 305 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 364
DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN APWIITVGASTVDREFYSP
Sbjct: 304 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 363
Query: 365 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 424
VILGNG KI+GLSVAPSKL+R KMYPLVYA DI KPHVP+N+SG C+AGSLSH+KA+GKI
Sbjct: 364 VILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKGKI 423
Query: 425 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 484
VLCYR +GISRYAGSLEVKRSGGAGMILG VPAVG +LHADPHFVPATAVSY+DAN ILK
Sbjct: 424 VLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRILK 483
Query: 485 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 544
YIKS NPTATIVPPITIYGSRPAPAMANF+SRGP+ +DPH LKPDITAPGVDILAAWS
Sbjct: 484 YIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWSE 543
Query: 545 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 604
+DSPTKLPKS DPR+V YN+YSGTSMSCPHV+AAAALLRAIHPTWSQAAIRSALMTTATT
Sbjct: 544 KDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTATT 603
Query: 605 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMAHS 664
TNKYG PITDD++ ++PATPFS+GSGHFRP+KAADPGLVYDANYTDYL +LC LKM +S
Sbjct: 604 TNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM-NS 663
Query: 665 IDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKSAYFFRASAPAG 724
ID SF CP RA HDLNYPS+A+P+L+ GGVR+ RTV NV GG KS YFF + AP G
Sbjct: 664 IDRSFKCPPRALHPHDLNYPSIAIPKLK---GGVRIKRTVTNVGGGGKSVYFFESWAPPG 723
Query: 725 VSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPIAIS 784
V+VRASPSVLYF R+GQRKRFTIT+SGK V G+GYSFGWFAW+DG+H+VRSPIAIS
Sbjct: 724 VAVRASPSVLYFDRVGQRKRFTITVSGK-----VKGNGYSFGWFAWSDGVHYVRSPIAIS 771
BLAST of MS010969 vs. NCBI nr
Match:
XP_023517293.1 (subtilisin-like protease SBT5.6 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 631/781 (80.79%), Postives = 697/781 (89.24%), Query Frame = 0
Query: 5 STFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTE 64
S+ CLLLLLLLLL +S + AYIVYFGEH GDK+W EIE HHSYL SVKDTE
Sbjct: 4 SSLFCLLLLLLLLLLPPSSCLA--QKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 63
Query: 65 EEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEE 124
+A+SSL+YSYKH+INGFAAVL+ ++ASKLSE++ VVSVI S++Y TTRSWEFSGVEE
Sbjct: 64 NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKRYGMHTTRSWEFSGVEE 123
Query: 125 DKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 184
DKPRL+DLVS+AK GKDVVI MLDSGVWPKSKSFSDKGMGP+P SW+GICQTGP FQS H
Sbjct: 124 DKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPGFQSPH 183
Query: 185 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 244
CNRKIIGARYY+KGYE++FGRLNET D+RSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 184 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 243
Query: 245 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 304
RGTASGGAPG RLAIYKVCWAIP QMK LGNVCFDTDVLAAMDDAI+DGVDVLSLSIGK+
Sbjct: 244 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 303
Query: 305 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 364
DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN APWIITVGASTVDREFYSP
Sbjct: 304 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 363
Query: 365 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 424
VILGNG KI+GLSVAPSKL+R KMYPLVYA DI KPHVP+N+SG C+AGSLSH+KA+GKI
Sbjct: 364 VILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKGKI 423
Query: 425 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 484
VLCYR +GISRYAGSLEVKRSGGAGMILG VPAVG +LHADPHFVPATAVSY+DAN ILK
Sbjct: 424 VLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRILK 483
Query: 485 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 544
YIKS NPTATIVPPITIYGSRPAPAMA F+SRGP+ +DPH LKPDITAPGVDILAAWS
Sbjct: 484 YIKSHRNPTATIVPPITIYGSRPAPAMAYFTSRGPSLVDPHFLKPDITAPGVDILAAWSE 543
Query: 545 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 604
+DSPTKLPKS DPR+V YN+YSGTSMSCPHV+AAAALLRAIHPTWSQAAIRSALMTTATT
Sbjct: 544 KDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTATT 603
Query: 605 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMAHS 664
TNKYG PITDD++ ++PATPFS+GSGHFRP+KAADPGLVYDANYTDYL +LC LKM +S
Sbjct: 604 TNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM-NS 663
Query: 665 IDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKSAYFFRASAPAG 724
IDPSF CP RA HDLNYPS+A+P+L+ GGVR+ RTV NV GG K+ YFF + AP G
Sbjct: 664 IDPSFKCPPRALHPHDLNYPSIAIPKLK---GGVRIKRTVTNVGGGGKTVYFFESWAPPG 723
Query: 725 VSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPIAIS 784
V+VRASPSVLYF R+GQRKRFT+T+SGK V G+GYSFGWFAW+DGIH+VRSPIAIS
Sbjct: 724 VAVRASPSVLYFDRVGQRKRFTVTVSGK-----VKGNGYSFGWFAWSDGIHYVRSPIAIS 773
BLAST of MS010969 vs. ExPASy Swiss-Prot
Match:
Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)
HSP 1 Score: 848.6 bits (2191), Expect = 5.7e-245
Identity = 454/809 (56.12%), Postives = 574/809 (70.95%), Query Frame = 0
Query: 1 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 60
M+K ++ LL L+ LL S ++ YIVYFGEH+GDKA+HEIE HHSYL SV
Sbjct: 1 MKKLTSLFPLLFLIPLL------ASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSV 60
Query: 61 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRS--EKYSTQTTRSWE 120
K++EE+AR+SL+YSYKHSINGFAA L+ ++ASKL ++ VVSV +S KY TTRSWE
Sbjct: 61 KESEEDARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWE 120
Query: 121 FSGVEEDKP------RLND----------LVSRAKFGKDVVIAMLDSGVWPKSKSFSDKG 180
F G+EE++ R ND + +AK G +++ +LDSGVWP+SKSF+DKG
Sbjct: 121 FVGLEEEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKG 180
Query: 181 MGPIPNSWRGICQTGPAFQSAHCNRKIIGARYYLKGYENYFGRLNET--EDYRSPCDKDG 240
MGP+P SW+GICQTG AF S+HCNRKIIGARYY+KGYE Y+G N T +D+ SP D DG
Sbjct: 181 MGPVPKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDG 240
Query: 241 HGSHTASTAGGRRVFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDT 300
HGSHTASTA GRRV SA GG A+G+ASGGAP ARLAIYK CWA P K+ GN+C +
Sbjct: 241 HGSHTASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEE 300
Query: 301 DVLAAMDDAIADGVDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPS 360
D+LAA+DDAIADGV V+S+SIG +P+ +T DGIA+GALHAVK++IVV+ SAGN GP P
Sbjct: 301 DMLAAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPG 360
Query: 361 ALSNFAPWIITVGASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKP 420
LSN APWIITVGAST+DR F ++LGNG+ I+ S+ + K K PLVYA +++ P
Sbjct: 361 TLSNLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSI--TAFKMDKFAPLVYASNVVVP 420
Query: 421 HVPKNQSGVCVAGSLSHEKARGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGR 480
+ N++ C+ SL E GK+VLC R G SR +EVKR+GGAGMILGN+ A G
Sbjct: 421 GIALNETSQCLPNSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGN 480
Query: 481 KLHADPHFVPATAVSYEDANIILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPN 540
++ +D HFVP V+ + IL+YIK+ NP A I P T+Y + AP+M FSSRGPN
Sbjct: 481 EVPSDSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPN 540
Query: 541 PIDPHILKPDITAPGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAA 600
+DP+ILKPDITAPG+ ILAAWSG DSP+K+ S+D RV YN+YSGTSMSCPHVA A A
Sbjct: 541 VVDPNILKPDITAPGLYILAAWSGADSPSKM--SVDQRVAGYNIYSGTSMSCPHVAGAIA 600
Query: 601 LLRAIHPTWSQAAIRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAAD 660
LL+AIHP WS AAIRSALMTTA TN +PI D PA PF+ GSGHFRP+KAAD
Sbjct: 601 LLKAIHPKWSSAAIRSALMTTAWMTNDKKKPIQDTT---GLPANPFALGSGHFRPTKAAD 660
Query: 661 PGLVYDANYTDYLVHLCALKMAHSIDPSFTCPAR-ARAHDLNYPSVAVPRLRGGGGGVRV 720
PGLVYDA+Y YL++ C++ + +IDP+F CP++ ++ NYPS+AVP L+ V V
Sbjct: 661 PGLVYDASYRAYLLYGCSVNIT-NIDPTFKCPSKIPPGYNHNYPSIAVPNLK---KTVTV 720
Query: 721 VRTVRNV--GGSKSAYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTITISG-KAEIGGV 780
RTV NV G S S Y F P+G+SV+A P++L F+R+GQ++RF I I K ++
Sbjct: 721 KRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNA 780
Query: 781 DGSG-YSFGWFAWTDGIHFVRSPIAISSA 785
G Y FGWF+WTD +H VRSPIA+S A
Sbjct: 781 TEKGQYQFGWFSWTDKVHVVRSPIAVSLA 791
BLAST of MS010969 vs. ExPASy Swiss-Prot
Match:
F4KEL0 (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana OX=3702 GN=SBT5.5 PE=3 SV=2)
HSP 1 Score: 774.2 bits (1998), Expect = 1.4e-222
Identity = 430/806 (53.35%), Postives = 536/806 (66.50%), Query Frame = 0
Query: 7 FVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTEEE 66
F + L LLL S ++ YIVYFGEH+GDKA+HEIEA HHSYL SVK+TEE+
Sbjct: 5 FGIFIFLSLLLFLVPLLASCTKEKQVYIVYFGEHKGDKAFHEIEAHHHSYLQSVKETEED 64
Query: 67 ARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSE--KYSTQTTRSWEFSGVEE 126
A SSL+Y SINGFAA L+ ++AS+L E+ VVSV +S+ KY TTRSWEF G++E
Sbjct: 65 ATSSLLYRRASSINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKE 124
Query: 127 ----------DKPR----LND-------LVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGM 186
D PR +ND + AK G V++ ++DSGVWP+S+SF DKGM
Sbjct: 125 EEGEDYRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGM 184
Query: 187 GPIPNSWRGICQTGPAFQSAHCNRKIIGARYYLKGYENYFGRLN--ETEDYRSPCDKDGH 246
GPIP SW+GICQTG AF S+HCNRKIIGARYY +GYE Y+G N +D+ SP D DGH
Sbjct: 185 GPIPESWKGICQTGVAFNSSHCNRKIIGARYYARGYERYYGPFNAEANKDFLSPRDADGH 244
Query: 247 GSHTASTAGGRRVFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTD 306
GSHTASTA GRRV VSA GG+A GTASGGA ARLA+YK CWA+P + K N CFD D
Sbjct: 245 GSHTASTAVGRRVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDED 304
Query: 307 VLAAMDDAIADGVDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSA 366
+LAA DDAIADGV+V+S+SIG +P+ Y +DGIAIGALHAVK+DIVV+ SAGN GP
Sbjct: 305 MLAAFDDAIADGVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARET 364
Query: 367 LSNFAPWIITVGASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPH 426
LSN APWIITVGAS++DR F + LG+G+ S+ + LK PLVYA D++ P
Sbjct: 365 LSNPAPWIITVGASSLDRFFVGRLELGDGYVFESDSL--TTLKMDNYAPLVYAPDVVVPG 424
Query: 427 VPKNQSGVCVAGSLSHEKARGKIVLCYRAEGISRYAG-SLEVKRSGGAGMILGNVPAVGR 486
V +N + +C+ +LS + RGK+VLC R G G LEVKR+GG GMIL N
Sbjct: 425 VSRNDAMLCLPNALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDND 484
Query: 487 KLHADPHFVPATAVSYEDANIILKYIKSRTNPTATIVPPITI-YGSRPAPAMANFSSRGP 546
+ HFVP V + IL YI + P A I P T+ Y ++P ++ FSSR P
Sbjct: 485 AFDVESHFVPTALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPFSSRAP 544
Query: 547 NPIDPHILKPDITAPGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAA 606
N +D +ILKPDI APG++ILAAWSG DS +K S+D RV+ YNL SGTSMSCPHVA A
Sbjct: 545 NWVDANILKPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAI 604
Query: 607 ALLRAIHPTWSQAAIRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAA 666
ALL+++HPTWS AAIRSALMTTA+ TN+ PI D +PA PF+ GS HFRP+KAA
Sbjct: 605 ALLKSMHPTWSSAAIRSALMTTASMTNEDNEPIQD---YDGSPANPFALGSRHFRPTKAA 664
Query: 667 DPGLVYDANYTDYLVHLCALKMAHSIDPSFTCPAR-ARAHDLNYPSVAVPRLRGGGGGVR 726
PGLVYDA+Y YL++ C++ + ++DP+F CP+R ++LNYPS+++P L G R
Sbjct: 665 SPGLVYDASYQSYLLYCCSVGLT-NLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTR 724
Query: 727 VVRTVRNVGGSKSAYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTI--TISGKAEIGGV 783
V V G S S Y F A P GV V+A P+VL F ++GQ+KRF I T G
Sbjct: 725 TVTCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEA 784
BLAST of MS010969 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 602.1 bits (1551), Expect = 9.3e-171
Identity = 350/784 (44.64%), Postives = 476/784 (60.71%), Query Frame = 0
Query: 11 LLLLLLLLFQQASPSPNNDQAAYIVYFGEHR-----GDKAWHEIEATHHSYLTSVKDTEE 70
LLLLLL+ + D ++Y+VYFG H + A ++ TH+ +L S + E
Sbjct: 10 LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69
Query: 71 EARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEED 130
A ++ YSY INGFAA L + A ++S+ VVSV ++ TTRSW+F G+E +
Sbjct: 70 RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129
Query: 131 K-PRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 190
+ + +A+FG+D +IA LD+GVWP+SKSF D+G+GPIP+ W+GICQ + H
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQ-NQKDATFH 189
Query: 191 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 250
CNRK+IGARY+ KGY G LN + D SP D DGHGSHT STA G V VS F G
Sbjct: 190 CNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVPGVSIF-GQG 249
Query: 251 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 310
GTA GG+P AR+A YKVCW + GN C+D DVLAA D AI DG DV+S+S+G
Sbjct: 250 NGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGADVISVSLG-G 309
Query: 311 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 370
+P ++ +D +AIG+ HA KK IVV CSAGN GP S +SN APW ITVGAST+DREF S
Sbjct: 310 EPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASN 369
Query: 371 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 430
++LGNG +G S++ + L K YP++ + + + + +C GSL K +GKI
Sbjct: 370 LVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKI 429
Query: 431 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 490
++C R + R V GG GM+L N G L ADPH +PAT ++ +D+ + +
Sbjct: 430 LVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSR 489
Query: 491 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 550
YI P A I P T G +PAP MA+FSS+GP+ + P ILKPDITAPGV ++AA++G
Sbjct: 490 YISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTG 549
Query: 551 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 610
SPT + DPR + +N SGTSMSCPH++ A LL+ +P+WS AAIRSA+MTTAT
Sbjct: 550 AVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATI 609
Query: 611 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCAL----- 670
+ PI + + ATPFS+G+GH +P+ A +PGLVYD DYL LC+L
Sbjct: 610 MDDIPGPIQNAT---NMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNAS 669
Query: 671 KMAHSIDPSFTCPA-RARAHDLNYPSVAVPRLRGGGGGVRVVRTVRNVGGSKSAYFFRAS 730
+++ +FTC + + +LNYPS+ VP L V V RTV+NV G S Y + +
Sbjct: 670 QISVFSGNNFTCSSPKISLVNLNYPSITVPNLT--SSKVTVSRTVKNV-GRPSMYTVKVN 729
Query: 731 APAGVSVRASPSVLYFSRMGQRKRF-TITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRS 782
P GV V P+ L F+++G++K F I + K + GY FG W+D H VRS
Sbjct: 730 NPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV----AKGYVFGELVWSDKKHRVRS 770
BLAST of MS010969 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 592.0 bits (1525), Expect = 9.6e-168
Identity = 352/777 (45.30%), Postives = 482/777 (62.03%), Query Frame = 0
Query: 13 LLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTEEEARSSLI 72
LLL L F S S ++DQ YIV+ + + ++ + S L S+ D+ E L+
Sbjct: 13 LLLCLGFCHVS-SSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-----LL 72
Query: 73 YSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEEDKPRLNDL 132
Y+Y+++I+GF+ L+ EEA L V+SV+ +Y TTR+ F G++E DL
Sbjct: 73 YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE---HTADL 132
Query: 133 VSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAHCNRKIIGA 192
A DVV+ +LD+GVWP+SKS+SD+G GPIP+SW+G C+ G F ++ CNRK+IGA
Sbjct: 133 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 192
Query: 193 RYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVARGTASGGA 252
R++ +GYE+ G ++E+++ RSP D DGHG+HT+STA G V S G A GTA G A
Sbjct: 193 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASGTARGMA 252
Query: 253 PGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKADPYNYTDD 312
P AR+A+YKVCW LG CF +D+LAA+D AIAD V+VLS+S+G +Y D
Sbjct: 253 PRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGMSDYYRD 312
Query: 313 GIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSPVILGNGFK 372
G+AIGA A+++ I+VSCSAGN GP+ S+LSN APWI TVGA T+DR+F + ILGNG
Sbjct: 313 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 372
Query: 373 IRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKIVLCYRAEG 432
G+S+ + K+ P +YAG+ +C+ G+L EK +GKIV+C R
Sbjct: 373 FTGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIVMCDRGIN 432
Query: 433 ISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILKYIKSRTNP 492
G + VK +GG GMIL N A G +L AD H +PAT V + +II Y+ + NP
Sbjct: 433 ARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 492
Query: 493 TATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSGEDSPTKLP 552
TA+I T+ G +P+P +A FSSRGPN I P+ILKPD+ APGV+ILAAW+G PT L
Sbjct: 493 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGL- 552
Query: 553 KSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATTTNKYGRPI 612
+ D R V +N+ SGTSMSCPHV+ AALL+++HP WS AAIRSALMTTA T K G+P+
Sbjct: 553 -ASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPL 612
Query: 613 TDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK----MAHSID-P 672
D A+ P+TPF +G+GH P+ A +PGL+YD DYL LCAL S+
Sbjct: 613 LDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR 672
Query: 673 SFTC-PARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNVGGSKSAYFFRASAPAGVSV 732
++TC P+++ DLNYPS AV G G + RTV +VGG+ + S GV +
Sbjct: 673 NYTCDPSKSYSVADLNYPSFAVN--VDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKI 732
Query: 733 RASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPIAIS 783
P+VL F ++K +T+T + + GS SFG W+DG H V SP+AIS
Sbjct: 733 SVEPAVLNFKEANEKKSYTVTFT--VDSSKPSGSN-SFGSIEWSDGKHVVGSPVAIS 755
BLAST of MS010969 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 581.3 bits (1497), Expect = 1.7e-164
Identity = 341/783 (43.55%), Postives = 460/783 (58.75%), Query Frame = 0
Query: 11 LLLLLLLLFQQASPSPNNDQAAYIVYFGEHR-----GDKAWHEIEATHHSYLTSVKDTEE 70
LLLL+ L F A + +YIVY G H + +H ++L S + E
Sbjct: 24 LLLLVTLFFSPAFAL----KKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHE 83
Query: 71 EARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEED 130
A+ ++ YSYK INGFAA+L EA+++++ VVSV ++ TT SW F + ++
Sbjct: 84 NAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN 143
Query: 131 -KPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 190
+ L ++A +G+D +IA LD+GVWP+SKSFSD+G G +P W+G C +
Sbjct: 144 GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-----KDVP 203
Query: 191 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 250
CNRK+IGARY+ KGY Y G L Y + D DGHGSHT STA G V + F G+
Sbjct: 204 CNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF-GIG 263
Query: 251 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 310
GTASGG+P AR+A YKVCW + G CFD D+LAA++ AI DGVDVLS S+G
Sbjct: 264 NGTASGGSPKARVAAYKVCWP-----PVDGAECFDADILAAIEAAIEDGVDVLSASVG-G 323
Query: 311 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 370
D +Y DGIAIG+ HAVK + V CSAGN GP +SN APW+ITVGAS++DREF +
Sbjct: 324 DAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAF 383
Query: 371 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 430
V L NG +G S++ L +KMY L+ A D + + +C GSL +K +GKI
Sbjct: 384 VELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 443
Query: 431 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 490
++C R + +R ++ +G AGM+L N A G ++ +D H +PA+ + Y+D +
Sbjct: 444 LVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFS 503
Query: 491 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 550
Y+ S +P I P ++PAP MA+FSSRGPN I P ILKPDITAPGV+I+AA++
Sbjct: 504 YLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTE 563
Query: 551 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 610
PT L D R +N SGTSMSCPH++ LL+ +HP WS AAIRSA+MTT+ T
Sbjct: 564 ATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRT 623
Query: 611 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMAHS 670
N +P+ D++ A PFSYGSGH +P+KAA PGLVYD DYL LCA+ ++
Sbjct: 624 RNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNT 683
Query: 671 I------DPSFTCPARARAHDLNYPSVAVPRLRGGGGGVRVVRTVRNVGGSKSAYFFRAS 730
+ DP +TC A D NYPS+ VP L G + V R ++NV G + Y R
Sbjct: 684 VVQLFAEDPQYTCRQGANLLDFNYPSITVPNLT---GSITVTRKLKNV-GPPATYNARFR 743
Query: 731 APAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSP 782
P GV V P L F++ G+ K F +T+ V SGY FG WTD H+VRSP
Sbjct: 744 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLP----VTPSGYVFGELTWTDSHHYVRSP 774
BLAST of MS010969 vs. ExPASy TrEMBL
Match:
A0A6J1CJX5 (subtilisin-like protease SBT5.6 OS=Momordica charantia OX=3673 GN=LOC111012300 PE=3 SV=1)
HSP 1 Score: 1558.9 bits (4035), Expect = 0.0e+00
Identity = 780/785 (99.36%), Postives = 780/785 (99.36%), Query Frame = 0
Query: 1 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 60
MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV
Sbjct: 32 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 91
Query: 61 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFS 120
KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVV VIRSEKYSTQTTRSWEFS
Sbjct: 92 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVRVIRSEKYSTQTTRSWEFS 151
Query: 121 GVEEDKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAF 180
GVEEDKPRLNDLVSRAKFGKDVVI MLDSGVWPKSKSFSDKGMGPIP SWRGICQTGPAF
Sbjct: 152 GVEEDKPRLNDLVSRAKFGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWRGICQTGPAF 211
Query: 181 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 240
QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF
Sbjct: 212 QSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAF 271
Query: 241 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 300
GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS
Sbjct: 272 GGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLS 331
Query: 301 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 360
IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE
Sbjct: 332 IGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDRE 391
Query: 361 FYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 420
FYSPVILGNGFKIRGLSVAPS LKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA
Sbjct: 392 FYSPVILGNGFKIRGLSVAPSMLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKA 451
Query: 421 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 480
RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN
Sbjct: 452 RGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDAN 511
Query: 481 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 540
IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA
Sbjct: 512 IILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILA 571
Query: 541 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 600
AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT
Sbjct: 572 AWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMT 631
Query: 601 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 660
TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK
Sbjct: 632 TATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK 691
Query: 661 MAHSIDPSFTCPARARAHDLNYPSVAVPRLR-GGGGGVRVVRTVRNVGGSKSAYFFRASA 720
MAHSIDPSFTCPARARAHDLNYPSVAVPRLR GGGGGVRVVRTVRNVGGSKSAYFFRASA
Sbjct: 692 MAHSIDPSFTCPARARAHDLNYPSVAVPRLRGGGGGGVRVVRTVRNVGGSKSAYFFRASA 751
Query: 721 PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI 780
PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI
Sbjct: 752 PAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPI 811
Query: 781 AISSA 785
AISSA
Sbjct: 812 AISSA 816
BLAST of MS010969 vs. ExPASy TrEMBL
Match:
A0A0A0KHZ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520430 PE=3 SV=1)
HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 639/795 (80.38%), Postives = 710/795 (89.31%), Query Frame = 0
Query: 1 MEKYSTFVC---LLLLLLLLL----FQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATH 60
MEK S+F+C LLLLLLLLL QQA+ S NN + AYIVYFGEH G+K+ EI+ H
Sbjct: 1 MEK-SSFLCHCRLLLLLLLLLIGLFIQQAASSSNNQKKAYIVYFGEHHGEKSIEEIKERH 60
Query: 61 HSYLTSVKDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQT 120
HSYL VK++EE+A+S L+Y+YKHSIN FAA+L+ ++ASKLS++D VVSVI S+KY +T
Sbjct: 61 HSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSDLDEVVSVIESKKYRMET 120
Query: 121 TRSWEFSGVEEDKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGI 180
TRSWEFSGVEEDKP +NDLVSRA +GKDVVI MLDSGVWPKSKSFSDKGMGPIP SW+GI
Sbjct: 121 TRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPKSKSFSDKGMGPIPKSWKGI 180
Query: 181 CQTGPAFQSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRR 240
CQTGPAFQSAHCNRKIIGARYYLKGYE++FGRLN+T DYRSPCDKDGHGSHTAS AGGRR
Sbjct: 181 CQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNKTADYRSPCDKDGHGSHTASIAGGRR 240
Query: 241 VFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADG 300
V+NVSAFGGVA GTASGGAP ARLAIYKVCWAIP QMK LGNVCFDTD+LAAMDDAIADG
Sbjct: 241 VYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIADG 300
Query: 301 VDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVG 360
VDVLSLSIGK++PYNYTDDG+AIGALHAVKKDIVVSCSAGNYGPTPSALSN APWIITVG
Sbjct: 301 VDVLSLSIGKSEPYNYTDDGMAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITVG 360
Query: 361 ASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAG 420
ASTVDREFYSPVILGNG KI+GLSVAPSKL+RKKMYPLVYAGDIM PH P+NQSG+CVAG
Sbjct: 361 ASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVAG 420
Query: 421 SLSHEKARGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATA 480
SLSHEKA+GKIVLC+R EGISR+AGSLEV+RSGGAGMILGNVPAVGR+ HADPHFVPATA
Sbjct: 421 SLSHEKAKGKIVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPATA 480
Query: 481 VSYEDANIILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITA 540
VSYEDANIILKYIKSR NPTATIVPP+TIYGSRPAPAMANFSSRGPNPIDPH LKPDITA
Sbjct: 481 VSYEDANIILKYIKSRKNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDITA 540
Query: 541 PGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAA 600
PGVDILAAWS +DSPTKLPK LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHPTWSQAA
Sbjct: 541 PGVDILAAWSEQDSPTKLPKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQAA 600
Query: 601 IRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYL 660
IRSALMTT+TT NKYG+PITDD++ ++PATPFS+GSGHFRPSKAADPGLVYD+NYTDYL
Sbjct: 601 IRSALMTTSTTNNKYGQPITDDSTLDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDYL 660
Query: 661 VHLCALKMAHSIDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKS 720
+LC LKM +SIDPSF CP RA HDLNYPS+AVP+LR VR+ RTV NV GG K+
Sbjct: 661 HYLCGLKM-NSIDPSFKCPPRALHPHDLNYPSIAVPQLR---NVVRIKRTVTNVGGGGKN 720
Query: 721 AYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTITISGKA---EIGGVDGSGYSFGWFAW 780
YFF++ AP GV+V ASP++LYF+R+G+RK+FTITIS K G YSFGWFAW
Sbjct: 721 VYFFKSEAPRGVAVSASPNILYFNRVGERKKFTITISRKVNNNNRSSKKGEDYSFGWFAW 780
Query: 781 TDGIHFVRSPIAISS 784
+DGIH+VRSPIA+SS
Sbjct: 781 SDGIHYVRSPIAVSS 790
BLAST of MS010969 vs. ExPASy TrEMBL
Match:
A0A6J1EMK8 (subtilisin-like protease SBT5.6 OS=Cucurbita moschata OX=3662 GN=LOC111433935 PE=3 SV=1)
HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 632/781 (80.92%), Postives = 697/781 (89.24%), Query Frame = 0
Query: 5 STFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTE 64
S+ CLLLLLLLL S + AYIVYFGEH GDK+W EIE HHSYL SVKDTE
Sbjct: 4 SSLFCLLLLLLLL----PPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 63
Query: 65 EEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEE 124
+A+SSL+YSYKH+INGFAAVL+ ++ASKLSE++ VVSVI S+KY TTRSWEFSGVEE
Sbjct: 64 NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKKYGMHTTRSWEFSGVEE 123
Query: 125 DKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 184
DKPRL+DLVS+AK GKDVVI MLDSGVWPKSKSFSDKGMGP+P SW+GICQTGPAF+S+H
Sbjct: 124 DKPRLSDLVSKAKAGKDVVIGMLDSGVWPKSKSFSDKGMGPVPKSWKGICQTGPAFESSH 183
Query: 185 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 244
CNRKIIGARYY+KGYE++FGRLNET D+RSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 184 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 243
Query: 245 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 304
RGTASGGAPG RLAIYKVCWAIP QMK LGNVCFDTDVLAAMDDAI+DGVDVLSLSIGK+
Sbjct: 244 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 303
Query: 305 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 364
DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN APWIITVGASTVDREFYSP
Sbjct: 304 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 363
Query: 365 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 424
VILGNG KI+GLSVAPSKL+R KMYPLVYA DI KPHVP+N+SG C+AGSLSH+KA+GKI
Sbjct: 364 VILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPRNESGSCIAGSLSHKKAKGKI 423
Query: 425 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 484
VLCYR +GISRYAGSLEVKRSGGAGMILG VPAVG +LHADPHFVPATAVSY+DAN ILK
Sbjct: 424 VLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRILK 483
Query: 485 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 544
YIKS NPTATIVPPITIYGSRPAPAMANF+SRGP+ +DPH LKPDITAPGVDILAAWS
Sbjct: 484 YIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWSE 543
Query: 545 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 604
+DSPTKLPKS DPR+V YN+YSGTSMSCPHV+AAAALLRAIHPTWSQAAIRSALMTTATT
Sbjct: 544 KDSPTKLPKSFDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTATT 603
Query: 605 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMAHS 664
TNKYG PITDD++ ++PATPFS+GSGHFRP+KAADPGLVYDANYTDYL +LC LKM +S
Sbjct: 604 TNKYGHPITDDSTTDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM-NS 663
Query: 665 IDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKSAYFFRASAPAG 724
ID SF CP RA HDLNYPS+A+P+L+ GGVR+ RTV NV GG KS YFF + AP G
Sbjct: 664 IDRSFKCPPRALHPHDLNYPSIAIPKLK---GGVRIKRTVTNVGGGGKSVYFFESWAPPG 723
Query: 725 VSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPIAIS 784
V+VRASPSVLYF R+GQRKRFTIT+SGK V G+GYSFGWFAW+DG+H+VRSPIAIS
Sbjct: 724 VAVRASPSVLYFDRVGQRKRFTITVSGK-----VKGNGYSFGWFAWSDGVHYVRSPIAIS 771
BLAST of MS010969 vs. ExPASy TrEMBL
Match:
A0A1S3B024 (LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 OS=Cucumis melo OX=3656 GN=LOC103484415 PE=3 SV=1)
HSP 1 Score: 1266.9 bits (3277), Expect = 0.0e+00
Identity = 636/798 (79.70%), Postives = 706/798 (88.47%), Query Frame = 0
Query: 1 MEKYSTFVC----LLLLLLLLL----FQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEAT 60
MEK S+F+C LLLLLLLLL QQA+ S NN + AYIVYFGEH G+K+ EI+
Sbjct: 1 MEK-SSFLCHCHVLLLLLLLLLIGPFIQQAASSSNNQKKAYIVYFGEHHGEKSVEEIKER 60
Query: 61 HHSYLTSVKDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQ 120
HHSYL VK++EE+A+S L+Y+YKHSIN FAA+L+ ++ASKLSE+D VVSVI S+KY +
Sbjct: 61 HHSYLMYVKESEEDAKSCLLYNYKHSINAFAAILTPQQASKLSELDEVVSVIESKKYRME 120
Query: 121 TTRSWEFSGVEEDKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRG 180
TTRSWEFSGVEEDKP +NDLVSRA +GKDVVI MLDSGVWP SKSFSDKGMGP+P SW+G
Sbjct: 121 TTRSWEFSGVEEDKPTINDLVSRANYGKDVVIGMLDSGVWPNSKSFSDKGMGPLPKSWKG 180
Query: 181 ICQTGPAFQSAHCNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGR 240
ICQTGPAFQSAHCNRKIIGARYYLKGYE++FGRLNET DYRSPCDKDGHGSHTAS AGGR
Sbjct: 181 ICQTGPAFQSAHCNRKIIGARYYLKGYEHHFGRLNETADYRSPCDKDGHGSHTASIAGGR 240
Query: 241 RVFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIAD 300
RV+NVSAFGGVA GTASGGAP ARLAIYKVCWAIP QMK LGNVCFDTD+LAAMDDAIAD
Sbjct: 241 RVYNVSAFGGVAWGTASGGAPWARLAIYKVCWAIPNQMKALGNVCFDTDMLAAMDDAIAD 300
Query: 301 GVDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITV 360
GVDVLSLSIGK++PYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSN APWIITV
Sbjct: 301 GVDVLSLSIGKSEPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNVAPWIITV 360
Query: 361 GASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVA 420
GASTVDREFYSPVILGNG KI+GLSVAPSKL+RKKMYPLVYAGDIM PH P+NQSG+CVA
Sbjct: 361 GASTVDREFYSPVILGNGLKIKGLSVAPSKLERKKMYPLVYAGDIMNPHAPRNQSGLCVA 420
Query: 421 GSLSHEKARGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPAT 480
GSLSHEKA+GK VLC+R EGISR+AGSLEV+RSGGAGMILGNVPAVGR+ HADPHFVPAT
Sbjct: 421 GSLSHEKAKGKXVLCFRGEGISRFAGSLEVQRSGGAGMILGNVPAVGRRPHADPHFVPAT 480
Query: 481 AVSYEDANIILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDIT 540
AVSYEDAN+ILKYIKSRTNPTATIVPP+TIYGSRPAPAMANFSSRGPNPIDPH LKPDIT
Sbjct: 481 AVSYEDANVILKYIKSRTNPTATIVPPVTIYGSRPAPAMANFSSRGPNPIDPHFLKPDIT 540
Query: 541 APGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQA 600
APGVDILAAWS +DSPTKL K LDPR+V YNLYSGTSMSCPHV+AAAALLRAIHPTWSQA
Sbjct: 541 APGVDILAAWSEQDSPTKLSKYLDPRIVQYNLYSGTSMSCPHVSAAAALLRAIHPTWSQA 600
Query: 601 AIRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDY 660
AIRSALMTT+TT NK G+PITDD++ ++PATPFS+GSGHFRPSKAADPGLVYD+NYTDY
Sbjct: 601 AIRSALMTTSTTNNKSGQPITDDSTPDNSPATPFSFGSGHFRPSKAADPGLVYDSNYTDY 660
Query: 661 LVHLCALKMAHSIDPSFTCPAR-ARAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSK 720
L +LC LKM +SIDPSFTCP R HDLNYPS+AVP+LR VR+ RTV NV GG K
Sbjct: 661 LHYLCGLKM-NSIDPSFTCPPRPLHPHDLNYPSIAVPQLR---DVVRIKRTVTNVGGGGK 720
Query: 721 SAYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVD-----GSGYSFGW 780
S YFF++ AP GV+V ASP++LYF+R+G+RK+FTITIS K G YSFGW
Sbjct: 721 SVYFFKSEAPPGVAVSASPNILYFNRVGERKKFTITISRKVNSSSSSRRSKKGEEYSFGW 780
Query: 781 FAWTDGIHFVRSPIAISS 784
FAW+D IH+VRSPIA+S+
Sbjct: 781 FAWSDEIHYVRSPIAVST 793
BLAST of MS010969 vs. ExPASy TrEMBL
Match:
A0A6J1KU93 (subtilisin-like protease SBT5.6 OS=Cucurbita maxima OX=3661 GN=LOC111497250 PE=3 SV=1)
HSP 1 Score: 1256.9 bits (3251), Expect = 0.0e+00
Identity = 627/781 (80.28%), Postives = 690/781 (88.35%), Query Frame = 0
Query: 5 STFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTE 64
ST CLLLLLLL S + AYIVYFGEH GDK+W EIE HHSYL SVKDTE
Sbjct: 4 STLFCLLLLLLL-----PPSSCLAQKQAYIVYFGEHSGDKSWEEIEERHHSYLMSVKDTE 63
Query: 65 EEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEE 124
+A+SSL+YSYKH+INGFAAVL+ ++ASKLSE++ VVSVI S+ Y TTRSWEFSGV E
Sbjct: 64 NDAKSSLLYSYKHTINGFAAVLTQQQASKLSELEEVVSVIESKTYGMHTTRSWEFSGVVE 123
Query: 125 DKPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 184
DKPRL+DLVS+AK GKDVVI MLDSGVWPKSKSFSD GMGP+P SW+GICQTGP F+S H
Sbjct: 124 DKPRLSDLVSKAKSGKDVVIGMLDSGVWPKSKSFSDNGMGPVPKSWKGICQTGPGFESPH 183
Query: 185 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 244
CNRKIIGARYY+KGYE++FGRLNET D+RSPCD DGHGSHTAS AGGRRV+NVSAFGGVA
Sbjct: 184 CNRKIIGARYYVKGYEHHFGRLNETTDFRSPCDMDGHGSHTASIAGGRRVYNVSAFGGVA 243
Query: 245 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 304
RGTASGGAPG RLAIYKVCWAIP QMK LGNVCFDTDVLAAMDDAI+DGVDVLSLSIGK+
Sbjct: 244 RGTASGGAPGVRLAIYKVCWAIPNQMKSLGNVCFDTDVLAAMDDAISDGVDVLSLSIGKS 303
Query: 305 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 364
DPYNYTDDGIAIGALHAVK+DIVVSCSAGN GPTPS LSN APWIITVGASTVDREFYSP
Sbjct: 304 DPYNYTDDGIAIGALHAVKRDIVVSCSAGNSGPTPSTLSNVAPWIITVGASTVDREFYSP 363
Query: 365 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 424
VILGNG KI+GLSVAPSKL+R KMYPLVYA DI KPHVP+N+SG C+AGSLSH+KA+GKI
Sbjct: 364 VILGNGLKIKGLSVAPSKLRRNKMYPLVYAVDIQKPHVPQNESGSCIAGSLSHKKAKGKI 423
Query: 425 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 484
VLCYR +GISRYAGSLEVKRSGGAGMILG VPAVG +LHADPHFVPATAVSY+DAN ILK
Sbjct: 424 VLCYRGQGISRYAGSLEVKRSGGAGMILGLVPAVGARLHADPHFVPATAVSYDDANRILK 483
Query: 485 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 544
YIKS NPTATIVPPITIYGSRPAPAMANF+SRGP+ +DPH LKPDITAPGVDILAAWS
Sbjct: 484 YIKSHRNPTATIVPPITIYGSRPAPAMANFTSRGPSLVDPHFLKPDITAPGVDILAAWSE 543
Query: 545 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 604
+DSPT LPK LDPR+V YN+YSGTSMSCPHV+AAAALLRAIHPTWSQAAIRSALMTT+TT
Sbjct: 544 KDSPTXLPKFLDPRIVKYNIYSGTSMSCPHVSAAAALLRAIHPTWSQAAIRSALMTTSTT 603
Query: 605 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMAHS 664
TNKYG PITDD++ ++PATPFS+GSGHFRP+KAADPGLVYDANYTDYL +LC LKM +S
Sbjct: 604 TNKYGHPITDDSTSDNSPATPFSFGSGHFRPTKAADPGLVYDANYTDYLHYLCGLKM-NS 663
Query: 665 IDPSFTCPARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNV-GGSKSAYFFRASAPAG 724
ID SF CP RA HDLNYPS+A+P+L+ GGVR+ RTV NV GG KS YFF + AP G
Sbjct: 664 IDRSFKCPPRALHPHDLNYPSIAIPKLK---GGVRIKRTVTNVGGGGKSVYFFESWAPPG 723
Query: 725 VSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPIAIS 784
V+VRASPSVLYF R+GQRKRFT+T+SGK V GSGYSFGWFAW+DGIH+VRSPIAIS
Sbjct: 724 VAVRASPSVLYFDRVGQRKRFTVTVSGK-----VKGSGYSFGWFAWSDGIHYVRSPIAIS 770
BLAST of MS010969 vs. TAIR 10
Match:
AT5G45650.1 (subtilase family protein )
HSP 1 Score: 848.6 bits (2191), Expect = 4.0e-246
Identity = 454/809 (56.12%), Postives = 574/809 (70.95%), Query Frame = 0
Query: 1 MEKYSTFVCLLLLLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSV 60
M+K ++ LL L+ LL S ++ YIVYFGEH+GDKA+HEIE HHSYL SV
Sbjct: 1 MKKLTSLFPLLFLIPLL------ASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSV 60
Query: 61 KDTEEEARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRS--EKYSTQTTRSWE 120
K++EE+AR+SL+YSYKHSINGFAA L+ ++ASKL ++ VVSV +S KY TTRSWE
Sbjct: 61 KESEEDARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWE 120
Query: 121 FSGVEEDKP------RLND----------LVSRAKFGKDVVIAMLDSGVWPKSKSFSDKG 180
F G+EE++ R ND + +AK G +++ +LDSGVWP+SKSF+DKG
Sbjct: 121 FVGLEEEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKG 180
Query: 181 MGPIPNSWRGICQTGPAFQSAHCNRKIIGARYYLKGYENYFGRLNET--EDYRSPCDKDG 240
MGP+P SW+GICQTG AF S+HCNRKIIGARYY+KGYE Y+G N T +D+ SP D DG
Sbjct: 181 MGPVPKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDG 240
Query: 241 HGSHTASTAGGRRVFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDT 300
HGSHTASTA GRRV SA GG A+G+ASGGAP ARLAIYK CWA P K+ GN+C +
Sbjct: 241 HGSHTASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEE 300
Query: 301 DVLAAMDDAIADGVDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPS 360
D+LAA+DDAIADGV V+S+SIG +P+ +T DGIA+GALHAVK++IVV+ SAGN GP P
Sbjct: 301 DMLAAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPG 360
Query: 361 ALSNFAPWIITVGASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKP 420
LSN APWIITVGAST+DR F ++LGNG+ I+ S+ + K K PLVYA +++ P
Sbjct: 361 TLSNLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSI--TAFKMDKFAPLVYASNVVVP 420
Query: 421 HVPKNQSGVCVAGSLSHEKARGKIVLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGR 480
+ N++ C+ SL E GK+VLC R G SR +EVKR+GGAGMILGN+ A G
Sbjct: 421 GIALNETSQCLPNSLKPELVSGKVVLCLRGAG-SRIGKGMEVKRAGGAGMILGNIAANGN 480
Query: 481 KLHADPHFVPATAVSYEDANIILKYIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPN 540
++ +D HFVP V+ + IL+YIK+ NP A I P T+Y + AP+M FSSRGPN
Sbjct: 481 EVPSDSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPN 540
Query: 541 PIDPHILKPDITAPGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAA 600
+DP+ILKPDITAPG+ ILAAWSG DSP+K+ S+D RV YN+YSGTSMSCPHVA A A
Sbjct: 541 VVDPNILKPDITAPGLYILAAWSGADSPSKM--SVDQRVAGYNIYSGTSMSCPHVAGAIA 600
Query: 601 LLRAIHPTWSQAAIRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAAD 660
LL+AIHP WS AAIRSALMTTA TN +PI D PA PF+ GSGHFRP+KAAD
Sbjct: 601 LLKAIHPKWSSAAIRSALMTTAWMTNDKKKPIQDTT---GLPANPFALGSGHFRPTKAAD 660
Query: 661 PGLVYDANYTDYLVHLCALKMAHSIDPSFTCPAR-ARAHDLNYPSVAVPRLRGGGGGVRV 720
PGLVYDA+Y YL++ C++ + +IDP+F CP++ ++ NYPS+AVP L+ V V
Sbjct: 661 PGLVYDASYRAYLLYGCSVNIT-NIDPTFKCPSKIPPGYNHNYPSIAVPNLK---KTVTV 720
Query: 721 VRTVRNV--GGSKSAYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTITISG-KAEIGGV 780
RTV NV G S S Y F P+G+SV+A P++L F+R+GQ++RF I I K ++
Sbjct: 721 KRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNA 780
Query: 781 DGSG-YSFGWFAWTDGIHFVRSPIAISSA 785
G Y FGWF+WTD +H VRSPIA+S A
Sbjct: 781 TEKGQYQFGWFSWTDKVHVVRSPIAVSLA 791
BLAST of MS010969 vs. TAIR 10
Match:
AT5G45640.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 663.7 bits (1711), Expect = 1.8e-190
Identity = 379/744 (50.94%), Postives = 473/744 (63.58%), Query Frame = 0
Query: 78 SINGFAAVLSSEEASKLSEMDSVVSVIRSE--KYSTQTTRSWEFSGVEE----------D 137
SINGFAA L+ ++AS+L E+ VVSV +S+ KY TTRSWEF G++E D
Sbjct: 37 SINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGEDYRSDGD 96
Query: 138 KPR----LND-------LVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGIC 197
PR +ND + AK G V++ ++DSGVWP+S+SF DKGMGPIP SW+GIC
Sbjct: 97 APRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDSGVWPESRSFDDKGMGPIPESWKGIC 156
Query: 198 QTGPAFQSAHCNRKIIGARYYLKGYENYFGRLN--ETEDYRSPCDKDGHGSHTASTAGGR 257
QTG AF S+HCN RYY +GYE Y+G N +D+ SP D DGHGSHTASTA GR
Sbjct: 157 QTGVAFNSSHCN------RYYARGYERYYGPFNAEANKDFLSPRDADGHGSHTASTAVGR 216
Query: 258 RVFNVSAFGGVARGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIAD 317
RV VSA GG+A GTASGGA ARLA+YK CWA+P + K N CFD D+LAA DDAIAD
Sbjct: 217 RVDGVSALGGIAMGTASGGASLARLAVYKACWAVPNKEKYATNTCFDEDMLAAFDDAIAD 276
Query: 318 GVDVLSLSIGKADPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITV 377
GV+V+S+SIG +P+ Y +DGIAIGALHAVK+DIVV+ SAGN GP LSN APWIITV
Sbjct: 277 GVNVISISIGTVEPHTYLEDGIAIGALHAVKRDIVVAASAGNDGPARETLSNPAPWIITV 336
Query: 378 GASTVDREFYSPVILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVA 437
GAS++DR F + LG+G+ S+ + LK PLVYA D++ P V +N + +C+
Sbjct: 337 GASSLDRFFVGRLELGDGYVFESDSL--TTLKMDNYAPLVYAPDVVVPGVSRNDAMLCLP 396
Query: 438 GSLSHEKARGKIVLCYRAEGISRYAG-SLEVKRSGGAGMILGNVPAVGRKLHADPHFVPA 497
+LS + RGK+VLC R G G LEVKR+GG GMIL N + HFVP
Sbjct: 397 NALSPDHVRGKVVLCLRGYGSGSTIGKGLEVKRAGGVGMILAN-SRDNDAFDVESHFVPT 456
Query: 498 TAVSYEDANIILKYIKSRTNPTATIVPPIT----------IYGSRPAPAMANFSSRGPNP 557
V + IL YI + P A I P T +Y +PAP M +F
Sbjct: 457 ALVFSSTVDRILDYIYNTYEPVAFIKPAETVLYRNQPEDSVYPYKPAPFMTSF------- 516
Query: 558 IDPHILKPDITAPGVDILAAWSGEDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAAL 617
PDI APG++ILAAWSG DS +K S+D RV+ YNL SGTSMSCPHVA A AL
Sbjct: 517 ------LPDIIAPGLNILAAWSGADSASK--DSIDRRVLDYNLDSGTSMSCPHVAGAIAL 576
Query: 618 LRAIHPTWSQAAIRSALMTTATTTNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADP 677
L+++HPTWS AAIRSALMTTA+ TN+ PI D +PA PF+ GS HFRP+KAA P
Sbjct: 577 LKSMHPTWSSAAIRSALMTTASMTNEDNEPIQD---YDGSPANPFALGSRHFRPTKAASP 636
Query: 678 GLVYDANYTDYLVHLCALKMAHSIDPSFTCPAR-ARAHDLNYPSVAVPRLRGGGGGVRVV 737
GLVYDA+Y YL++ C++ + ++DP+F CP+R ++LNYPS+++P L G R V
Sbjct: 637 GLVYDASYQSYLLYCCSVGLT-NLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRTV 696
Query: 738 RTVRNVGGSKSAYFFRASAPAGVSVRASPSVLYFSRMGQRKRFTI--TISGKAEIGGVDG 783
V G S S Y F A P GV V+A P+VL F ++GQ+KRF I T G
Sbjct: 697 TCVGRTGNSTSVYVFNAQPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEARR 752
BLAST of MS010969 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 602.1 bits (1551), Expect = 6.6e-172
Identity = 350/784 (44.64%), Postives = 476/784 (60.71%), Query Frame = 0
Query: 11 LLLLLLLLFQQASPSPNNDQAAYIVYFGEHR-----GDKAWHEIEATHHSYLTSVKDTEE 70
LLLLLL+ + D ++Y+VYFG H + A ++ TH+ +L S + E
Sbjct: 10 LLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRE 69
Query: 71 EARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEED 130
A ++ YSY INGFAA L + A ++S+ VVSV ++ TTRSW+F G+E +
Sbjct: 70 RATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHN 129
Query: 131 K-PRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 190
+ + +A+FG+D +IA LD+GVWP+SKSF D+G+GPIP+ W+GICQ + H
Sbjct: 130 SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQ-NQKDATFH 189
Query: 191 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 250
CNRK+IGARY+ KGY G LN + D SP D DGHGSHT STA G V VS F G
Sbjct: 190 CNRKLIGARYFNKGYAAAVGHLNSSFD--SPRDLDGHGSHTLSTAAGDFVPGVSIF-GQG 249
Query: 251 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 310
GTA GG+P AR+A YKVCW + GN C+D DVLAA D AI DG DV+S+S+G
Sbjct: 250 NGTAKGGSPRARVAAYKVCWP-----PVKGNECYDADVLAAFDAAIHDGADVISVSLG-G 309
Query: 311 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 370
+P ++ +D +AIG+ HA KK IVV CSAGN GP S +SN APW ITVGAST+DREF S
Sbjct: 310 EPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASN 369
Query: 371 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 430
++LGNG +G S++ + L K YP++ + + + + +C GSL K +GKI
Sbjct: 370 LVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKI 429
Query: 431 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 490
++C R + R V GG GM+L N G L ADPH +PAT ++ +D+ + +
Sbjct: 430 LVCLRGQN-GRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSR 489
Query: 491 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 550
YI P A I P T G +PAP MA+FSS+GP+ + P ILKPDITAPGV ++AA++G
Sbjct: 490 YISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTG 549
Query: 551 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 610
SPT + DPR + +N SGTSMSCPH++ A LL+ +P+WS AAIRSA+MTTAT
Sbjct: 550 AVSPTN--EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATI 609
Query: 611 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCAL----- 670
+ PI + + ATPFS+G+GH +P+ A +PGLVYD DYL LC+L
Sbjct: 610 MDDIPGPIQNAT---NMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNAS 669
Query: 671 KMAHSIDPSFTCPA-RARAHDLNYPSVAVPRLRGGGGGVRVVRTVRNVGGSKSAYFFRAS 730
+++ +FTC + + +LNYPS+ VP L V V RTV+NV G S Y + +
Sbjct: 670 QISVFSGNNFTCSSPKISLVNLNYPSITVPNLT--SSKVTVSRTVKNV-GRPSMYTVKVN 729
Query: 731 APAGVSVRASPSVLYFSRMGQRKRF-TITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRS 782
P GV V P+ L F+++G++K F I + K + GY FG W+D H VRS
Sbjct: 730 NPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV----AKGYVFGELVWSDKKHRVRS 770
BLAST of MS010969 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 592.0 bits (1525), Expect = 6.8e-169
Identity = 352/777 (45.30%), Postives = 482/777 (62.03%), Query Frame = 0
Query: 13 LLLLLLFQQASPSPNNDQAAYIVYFGEHRGDKAWHEIEATHHSYLTSVKDTEEEARSSLI 72
LLL L F S S ++DQ YIV+ + + ++ + S L S+ D+ E L+
Sbjct: 13 LLLCLGFCHVS-SSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAE-----LL 72
Query: 73 YSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEEDKPRLNDL 132
Y+Y+++I+GF+ L+ EEA L V+SV+ +Y TTR+ F G++E DL
Sbjct: 73 YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE---HTADL 132
Query: 133 VSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAHCNRKIIGA 192
A DVV+ +LD+GVWP+SKS+SD+G GPIP+SW+G C+ G F ++ CNRK+IGA
Sbjct: 133 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 192
Query: 193 RYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVARGTASGGA 252
R++ +GYE+ G ++E+++ RSP D DGHG+HT+STA G V S G A GTA G A
Sbjct: 193 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLL-GYASGTARGMA 252
Query: 253 PGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKADPYNYTDD 312
P AR+A+YKVCW LG CF +D+LAA+D AIAD V+VLS+S+G +Y D
Sbjct: 253 PRARVAVYKVCW--------LGG-CFSSDILAAIDKAIADNVNVLSMSLG-GGMSDYYRD 312
Query: 313 GIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSPVILGNGFK 372
G+AIGA A+++ I+VSCSAGN GP+ S+LSN APWI TVGA T+DR+F + ILGNG
Sbjct: 313 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 372
Query: 373 IRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKIVLCYRAEG 432
G+S+ + K+ P +YAG+ +C+ G+L EK +GKIV+C R
Sbjct: 373 FTGVSLFKGEALPDKLLPFIYAGNASN----ATNGNLCMTGTLIPEKVKGKIVMCDRGIN 432
Query: 433 ISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILKYIKSRTNP 492
G + VK +GG GMIL N A G +L AD H +PAT V + +II Y+ + NP
Sbjct: 433 ARVQKGDV-VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 492
Query: 493 TATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSGEDSPTKLP 552
TA+I T+ G +P+P +A FSSRGPN I P+ILKPD+ APGV+ILAAW+G PT L
Sbjct: 493 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGL- 552
Query: 553 KSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATTTNKYGRPI 612
+ D R V +N+ SGTSMSCPHV+ AALL+++HP WS AAIRSALMTTA T K G+P+
Sbjct: 553 -ASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPL 612
Query: 613 TDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALK----MAHSID-P 672
D A+ P+TPF +G+GH P+ A +PGL+YD DYL LCAL S+
Sbjct: 613 LDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR 672
Query: 673 SFTC-PARA-RAHDLNYPSVAVPRLRGGGGGVRVVRTVRNVGGSKSAYFFRASAPAGVSV 732
++TC P+++ DLNYPS AV G G + RTV +VGG+ + S GV +
Sbjct: 673 NYTCDPSKSYSVADLNYPSFAVN--VDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKI 732
Query: 733 RASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSPIAIS 783
P+VL F ++K +T+T + + GS SFG W+DG H V SP+AIS
Sbjct: 733 SVEPAVLNFKEANEKKSYTVTFT--VDSSKPSGSN-SFGSIEWSDGKHVVGSPVAIS 755
BLAST of MS010969 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 581.3 bits (1497), Expect = 1.2e-165
Identity = 341/783 (43.55%), Postives = 460/783 (58.75%), Query Frame = 0
Query: 11 LLLLLLLLFQQASPSPNNDQAAYIVYFGEHR-----GDKAWHEIEATHHSYLTSVKDTEE 70
LLLL+ L F A + +YIVY G H + +H ++L S + E
Sbjct: 24 LLLLVTLFFSPAFAL----KKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHE 83
Query: 71 EARSSLIYSYKHSINGFAAVLSSEEASKLSEMDSVVSVIRSEKYSTQTTRSWEFSGVEED 130
A+ ++ YSYK INGFAA+L EA+++++ VVSV ++ TT SW F + ++
Sbjct: 84 NAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN 143
Query: 131 -KPRLNDLVSRAKFGKDVVIAMLDSGVWPKSKSFSDKGMGPIPNSWRGICQTGPAFQSAH 190
+ L ++A +G+D +IA LD+GVWP+SKSFSD+G G +P W+G C +
Sbjct: 144 GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH-----KDVP 203
Query: 191 CNRKIIGARYYLKGYENYFGRLNETEDYRSPCDKDGHGSHTASTAGGRRVFNVSAFGGVA 250
CNRK+IGARY+ KGY Y G L Y + D DGHGSHT STA G V + F G+
Sbjct: 204 CNRKLIGARYFNKGYLAYTG-LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVF-GIG 263
Query: 251 RGTASGGAPGARLAIYKVCWAIPKQMKILGNVCFDTDVLAAMDDAIADGVDVLSLSIGKA 310
GTASGG+P AR+A YKVCW + G CFD D+LAA++ AI DGVDVLS S+G
Sbjct: 264 NGTASGGSPKARVAAYKVCWP-----PVDGAECFDADILAAIEAAIEDGVDVLSASVG-G 323
Query: 311 DPYNYTDDGIAIGALHAVKKDIVVSCSAGNYGPTPSALSNFAPWIITVGASTVDREFYSP 370
D +Y DGIAIG+ HAVK + V CSAGN GP +SN APW+ITVGAS++DREF +
Sbjct: 324 DAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAF 383
Query: 371 VILGNGFKIRGLSVAPSKLKRKKMYPLVYAGDIMKPHVPKNQSGVCVAGSLSHEKARGKI 430
V L NG +G S++ L +KMY L+ A D + + +C GSL +K +GKI
Sbjct: 384 VELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 443
Query: 431 VLCYRAEGISRYAGSLEVKRSGGAGMILGNVPAVGRKLHADPHFVPATAVSYEDANIILK 490
++C R + +R ++ +G AGM+L N A G ++ +D H +PA+ + Y+D +
Sbjct: 444 LVCLRGDN-ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFS 503
Query: 491 YIKSRTNPTATIVPPITIYGSRPAPAMANFSSRGPNPIDPHILKPDITAPGVDILAAWSG 550
Y+ S +P I P ++PAP MA+FSSRGPN I P ILKPDITAPGV+I+AA++
Sbjct: 504 YLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTE 563
Query: 551 EDSPTKLPKSLDPRVVHYNLYSGTSMSCPHVAAAAALLRAIHPTWSQAAIRSALMTTATT 610
PT L D R +N SGTSMSCPH++ LL+ +HP WS AAIRSA+MTT+ T
Sbjct: 564 ATGPTDLDS--DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRT 623
Query: 611 TNKYGRPITDDASDHSAPATPFSYGSGHFRPSKAADPGLVYDANYTDYLVHLCALKMAHS 670
N +P+ D++ A PFSYGSGH +P+KAA PGLVYD DYL LCA+ ++
Sbjct: 624 RNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNT 683
Query: 671 I------DPSFTCPARARAHDLNYPSVAVPRLRGGGGGVRVVRTVRNVGGSKSAYFFRAS 730
+ DP +TC A D NYPS+ VP L G + V R ++NV G + Y R
Sbjct: 684 VVQLFAEDPQYTCRQGANLLDFNYPSITVPNLT---GSITVTRKLKNV-GPPATYNARFR 743
Query: 731 APAGVSVRASPSVLYFSRMGQRKRFTITISGKAEIGGVDGSGYSFGWFAWTDGIHFVRSP 782
P GV V P L F++ G+ K F +T+ V SGY FG WTD H+VRSP
Sbjct: 744 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLP----VTPSGYVFGELTWTDSHHYVRSP 774
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022142085.1 | 0.0e+00 | 99.36 | subtilisin-like protease SBT5.6 [Momordica charantia] | [more] |
| XP_038882517.1 | 0.0e+00 | 82.34 | subtilisin-like protease SBT5.6 [Benincasa hispida] | [more] |
| XP_004134922.1 | 0.0e+00 | 80.38 | subtilisin-like protease SBT5.6 isoform X2 [Cucumis sativus] >KGN49345.1 hypothe... | [more] |
| XP_022926980.1 | 0.0e+00 | 80.92 | subtilisin-like protease SBT5.6 [Cucurbita moschata] | [more] |
| XP_023517293.1 | 0.0e+00 | 80.79 | subtilisin-like protease SBT5.6 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9FK76 | 5.7e-245 | 56.12 | Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 S... | [more] |
| F4KEL0 | 1.4e-222 | 53.35 | Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana OX=3702 GN=SBT5.5 PE=3 S... | [more] |
| Q9ZSP5 | 9.3e-171 | 44.64 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| O65351 | 9.6e-168 | 45.30 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| F4JXC5 | 1.7e-164 | 43.55 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CJX5 | 0.0e+00 | 99.36 | subtilisin-like protease SBT5.6 OS=Momordica charantia OX=3673 GN=LOC111012300 P... | [more] |
| A0A0A0KHZ1 | 0.0e+00 | 80.38 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520430 PE=3 SV=1 | [more] |
| A0A6J1EMK8 | 0.0e+00 | 80.92 | subtilisin-like protease SBT5.6 OS=Cucurbita moschata OX=3662 GN=LOC111433935 PE... | [more] |
| A0A1S3B024 | 0.0e+00 | 79.70 | LOW QUALITY PROTEIN: subtilisin-like protease SBT5.6 OS=Cucumis melo OX=3656 GN=... | [more] |
| A0A6J1KU93 | 0.0e+00 | 80.28 | subtilisin-like protease SBT5.6 OS=Cucurbita maxima OX=3661 GN=LOC111497250 PE=3... | [more] |
