MS010881 (gene) Bitter gourd (TR) v1

Overview


Name	MS010881
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ethylene-responsive transcription factor 4-like
Location	scaffold35: 2713451 .. 2714161 (-)
RNA-Seq Expression	MS010881
Synteny	MS010881

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCCGAGGGAGAAGGCGGTGGCCGTCAAGGGTAACGGCGGGAGCGTTAAGGAAGTGCATTTCAGAGGAGTGAGGAAGAGGCCTTGGGGGAGATACGCCGCTGAGATCAGAGATCCGTGCAAGAAGAGCCGCGTCTGGCTCGGAACCTTCGACACGGCGGAGGAGGCCGCCCGTGCCTACGATACTGCCGCTAGAGAGTTCCGCGGCGCCAAGGCCAAGACCAATTTCCCCTTGCCCTCCCAAGATCTTCTAAACCTTAACAATAAGATTAACAACATCAACAATCAGAGCCCCAGTCAGAGTAGCACGGTTGAGTCCTCCAGCCGGGAGCAAGCTCTGATGGTTGATTCCTCCCCTTTGAATCTCAATCTCGGCCACGGAATCGCCGGAGCCATCAGTATTCCGTTCCAGCGCTACCAAATTCCCATGATCGGAAACGTTTTCGCCCGCGGCATACCGGCATCGAATCACCTTCTGTATTTCGATGCCGCTTTTCGTTCCGGAATGATCAAGAGTCATCATCAGAACCAACGGCTACCCTTCGATCACCATCACGAAGCGGTGAGTGATTTCCGGCGCGATTTCGCAAGCGGCGGCGTACAGAGTGACTCCGATTCCTCCTCCGTCGTCGACATGAACGGCCAGGACCTCAAGCCAAGAGGAAGCGGCCTCGATTTCGATCTGAACTTTCCCCCACCAGAAACCGCC

mRNA sequence

ATGGCGCCGAGGGAGAAGGCGGTGGCCGTCAAGGGTAACGGCGGGAGCGTTAAGGAAGTGCATTTCAGAGGAGTGAGGAAGAGGCCTTGGGGGAGATACGCCGCTGAGATCAGAGATCCGTGCAAGAAGAGCCGCGTCTGGCTCGGAACCTTCGACACGGCGGAGGAGGCCGCCCGTGCCTACGATACTGCCGCTAGAGAGTTCCGCGGCGCCAAGGCCAAGACCAATTTCCCCTTGCCCTCCCAAGATCTTCTAAACCTTAACAATAAGATTAACAACATCAACAATCAGAGCCCCAGTCAGAGTAGCACGGTTGAGTCCTCCAGCCGGGAGCAAGCTCTGATGGTTGATTCCTCCCCTTTGAATCTCAATCTCGGCCACGGAATCGCCGGAGCCATCAGTATTCCGTTCCAGCGCTACCAAATTCCCATGATCGGAAACGTTTTCGCCCGCGGCATACCGGCATCGAATCACCTTCTGTATTTCGATGCCGCTTTTCGTTCCGGAATGATCAAGAGTCATCATCAGAACCAACGGCTACCCTTCGATCACCATCACGAAGCGGTGAGTGATTTCCGGCGCGATTTCGCAAGCGGCGGCGTACAGAGTGACTCCGATTCCTCCTCCGTCGTCGACATGAACGGCCAGGACCTCAAGCCAAGAGGAAGCGGCCTCGATTTCGATCTGAACTTTCCCCCACCAGAAACCGCC

Coding sequence (CDS)

ATGGCGCCGAGGGAGAAGGCGGTGGCCGTCAAGGGTAACGGCGGGAGCGTTAAGGAAGTGCATTTCAGAGGAGTGAGGAAGAGGCCTTGGGGGAGATACGCCGCTGAGATCAGAGATCCGTGCAAGAAGAGCCGCGTCTGGCTCGGAACCTTCGACACGGCGGAGGAGGCCGCCCGTGCCTACGATACTGCCGCTAGAGAGTTCCGCGGCGCCAAGGCCAAGACCAATTTCCCCTTGCCCTCCCAAGATCTTCTAAACCTTAACAATAAGATTAACAACATCAACAATCAGAGCCCCAGTCAGAGTAGCACGGTTGAGTCCTCCAGCCGGGAGCAAGCTCTGATGGTTGATTCCTCCCCTTTGAATCTCAATCTCGGCCACGGAATCGCCGGAGCCATCAGTATTCCGTTCCAGCGCTACCAAATTCCCATGATCGGAAACGTTTTCGCCCGCGGCATACCGGCATCGAATCACCTTCTGTATTTCGATGCCGCTTTTCGTTCCGGAATGATCAAGAGTCATCATCAGAACCAACGGCTACCCTTCGATCACCATCACGAAGCGGTGAGTGATTTCCGGCGCGATTTCGCAAGCGGCGGCGTACAGAGTGACTCCGATTCCTCCTCCGTCGTCGACATGAACGGCCAGGACCTCAAGCCAAGAGGAAGCGGCCTCGATTTCGATCTGAACTTTCCCCCACCAGAAACCGCC

Protein sequence

MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSPLNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA

Homology

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BLAST of MS010881 vs. NCBI nr
Match: XP_022142019.1 (ethylene-responsive transcription factor 4-like [Momordica charantia])

HSP 1 Score: 473.8 bits (1218), Expect = 8.8e-130
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120
           YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP
Sbjct: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120

Query: 121 LNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRL 180
           LNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRL
Sbjct: 121 LNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRL 180

Query: 181 PFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA 238
           PFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA
Sbjct: 181 PFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA 237

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BLAST of MS010881 vs. NCBI nr
Match: KAG6604462.1 (Ethylene-responsive transcription factor 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 402.5 bits (1033), Expect = 2.5e-108
Identity = 209/248 (84.27%), Postives = 220/248 (88.71%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKANVGNVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNI-NNQSPSQSSTVESSSREQALMVDSS 120
           YD AAREFRG KAKTNFPLPS DLLNLNNKINNI NNQSPSQSSTVESSSREQ LMVDSS
Sbjct: 61  YDKAAREFRGVKAKTNFPLPSDDLLNLNNKINNINNNQSPSQSSTVESSSREQPLMVDSS 120

Query: 121 PLNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSH 180
           PLNLNL HG+     AGA++ PFQRYQIPMIG VF+RGIP SNH+LYFDAA R+GMIK+H
Sbjct: 121 PLNLNLSHGMPGLTAAGAVTFPFQRYQIPMIGEVFSRGIPPSNHVLYFDAALRAGMIKTH 180

Query: 181 -HQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRG----SGLDFDL 238
            H NQRL FDHHHEAVSDFRR++A GGVQSDSDSSSVVDMNGQDLKPR      GLD DL
Sbjct: 181 PHPNQRLRFDHHHEAVSDFRREYAGGGVQSDSDSSSVVDMNGQDLKPRSGGGVGGLDLDL 240

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BLAST of MS010881 vs. NCBI nr
Match: XP_023545040.1 (ethylene-responsive transcription factor 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 402.5 bits (1033), Expect = 2.5e-108
Identity = 209/248 (84.27%), Postives = 220/248 (88.71%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKANVGNVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNI-NNQSPSQSSTVESSSREQALMVDSS 120
           YD AAREFRG KAKTNFPLPS DLLNLNNKINNI NNQSPSQSSTVESSSREQ LMVDSS
Sbjct: 61  YDKAAREFRGVKAKTNFPLPSDDLLNLNNKINNINNNQSPSQSSTVESSSREQPLMVDSS 120

Query: 121 PLNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSH 180
           PLNLNL HG+     AGA++ PFQRYQIPMIG VF+RGIP SNH+LYFDAA R+GMIK+H
Sbjct: 121 PLNLNLAHGMPGLTAAGAVTFPFQRYQIPMIGEVFSRGIPPSNHVLYFDAALRAGMIKTH 180

Query: 181 -HQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRG----SGLDFDL 238
            H NQRL FDHHHEAVSDFRR++A GGVQSDSDSSSVVDMNGQDLKPR      GLD DL
Sbjct: 181 PHPNQRLRFDHHHEAVSDFRREYAGGGVQSDSDSSSVVDMNGQDLKPRSGGGVGGLDLDL 240

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BLAST of MS010881 vs. NCBI nr
Match: XP_022925660.1 (ethylene-responsive transcription factor 4-like [Cucurbita moschata] >KAG7034605.1 Ethylene-responsive transcription factor 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 402.1 bits (1032), Expect = 3.3e-108
Identity = 209/249 (83.94%), Postives = 220/249 (88.35%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKANVGNVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNI-NNQSPSQSSTVESSSREQALMVDSS 120
           YD AAREFRG KAKTNFPLPS DLLNLNNKINNI NNQSPSQSSTVESSSREQ LMVDSS
Sbjct: 61  YDKAAREFRGVKAKTNFPLPSDDLLNLNNKINNINNNQSPSQSSTVESSSREQPLMVDSS 120

Query: 121 PLNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSH 180
           PLNLNL HG+     AGA++ PFQRYQIPMIG VF+RGIP SNH+LYFDAA R+GMIK+H
Sbjct: 121 PLNLNLAHGMPGLTAAGAVTFPFQRYQIPMIGEVFSRGIPPSNHVLYFDAALRAGMIKTH 180

Query: 181 -HQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRG-----SGLDFD 238
            H NQRL FDHHHEAVSDFRR++A GGVQSDSDSSSVVDMNGQDLKPR       GLD D
Sbjct: 181 PHPNQRLRFDHHHEAVSDFRREYAGGGVQSDSDSSSVVDMNGQDLKPRSGGGGVGGLDLD 240

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BLAST of MS010881 vs. NCBI nr
Match: XP_022978752.1 (ethylene-responsive transcription factor 4-like [Cucurbita maxima])

HSP 1 Score: 399.4 bits (1025), Expect = 2.1e-107
Identity = 206/247 (83.40%), Postives = 218/247 (88.26%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKANVGNVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120
           YD AAREFRG KAKTNFP+PS DLLNLNNKIN  NNQSPSQSSTVESSSREQ LMVDSSP
Sbjct: 61  YDKAAREFRGVKAKTNFPMPSYDLLNLNNKINISNNQSPSQSSTVESSSREQPLMVDSSP 120

Query: 121 LNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSH- 180
           LNLNLGHG+      GA++ PFQRYQIPMIG VF+RGIP SNH+LYFDAA R+GMIK+H 
Sbjct: 121 LNLNLGHGMPGLTSTGAVTFPFQRYQIPMIGEVFSRGIPPSNHVLYFDAALRAGMIKTHP 180

Query: 181 HQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRG----SGLDFDLN 238
           H NQRL FDHH EAVSDFRR++A GGVQSDSDSSSVVDMNGQDLKPRG     GLD DLN
Sbjct: 181 HPNQRLRFDHHPEAVSDFRREYAGGGVQSDSDSSSVVDMNGQDLKPRGGVGVGGLDLDLN 240

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BLAST of MS010881 vs. ExPASy Swiss-Prot
Match: Q9LW49 (Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ERF4 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 6.3e-38
Identity = 111/239 (46.44%), Postives = 135/239 (56.49%), Query Frame = 0


Query: 10  VKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFR 69
           VK NG  VKEVH+RGVRKRPWGRYAAEIRDP KKSRVWLGTFDTAEEAA+AYDTAAREFR
Sbjct: 17  VKTNG--VKEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFR 76

Query: 70  GAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMV-DSSPLNLNLGHG 129
           G KAKTNFPLPS+             NQS S SST+ESSS E  +     +PL L+L   
Sbjct: 77  GPKAKTNFPLPSE-------------NQSTSHSSTMESSSGETGIHAPPHAPLELDLTRR 136

Query: 130 I-------------AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHH 189
           +             +G +  P   +Q P +       +P    +L FD+ +R G++    
Sbjct: 137 LGSVAADGGDNCRRSGEVGYPI-FHQQPTVA-----VLPNGQPVLLFDSLWRPGVVNRPQ 196

Query: 190 QNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPP 235
               +P       V+       +G   + SDSSSVV+ N  D K    G+D DLN  PP
Sbjct: 197 PYHVMPMAMGFNGVN-------AGVDPTVSDSSSVVEENQYDGK---RGIDLDLNLAPP 224

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BLAST of MS010881 vs. ExPASy Swiss-Prot
Match: Q40477 (Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 3.1e-37
Identity = 112/239 (46.86%), Postives = 137/239 (57.32%), Query Frame = 0


Query: 10  VKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFR 69
           VK +G  VKEVH+RGVRKRPWGRYAAEIRDP KKSRVWLGTFDTAEEAA+AYDTAAREFR
Sbjct: 17  VKTDG--VKEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFR 76

Query: 70  GAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMV-DSSPLNLNLGHG 129
           G KAKTNFP P++             NQSPS SSTVESSS E  +     +PL L+L   
Sbjct: 77  GPKAKTNFPSPTE-------------NQSPSHSSTVESSSGENGVHAPPHAPLELDLTRR 136

Query: 130 I-------------AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHH 189
           +             +G +  P   +Q P +       +P    +L FD+ +R+G++    
Sbjct: 137 LGSVAADGGDNCRRSGEVGYPI-FHQQPTVA-----VLPNGQPVLLFDSLWRAGVV---- 196

Query: 190 QNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPP 235
            N+  P+   H     F    A  G  + SDSSS V+ N  D K    G+D DLN  PP
Sbjct: 197 -NRPQPY---HVTPMGFNGVNAGVG-PTVSDSSSAVEENQYDGK---RGIDLDLNLAPP 222

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BLAST of MS010881 vs. ExPASy Swiss-Prot
Match: O80340 (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.0e-36
Identity = 115/238 (48.32%), Postives = 137/238 (57.56%), Query Frame = 0


Query: 16  SVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFRGAKAKT 75
           + KE+ +RGVRKRPWGRYAAEIRDP KK+RVWLGTFDTAEEAARAYDTAAR+FRGAKAKT
Sbjct: 19  NAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKAKT 78

Query: 76  NFPLPSQDLLNLNN-KINNINNQSPSQSSTVESSSREQALMVDSS-------PLNLNLGH 135
           NFP      L L++ K+     +SPSQSST++ +S    L+V S+        L L+LG 
Sbjct: 79  NFP----TFLELSDQKVPTGFARSPSQSSTLDCAS-PPTLVVPSATAGNVPPQLELSLGG 138

Query: 136 GIAGAISIPFQRYQIPM-----------IGNVFARGIPASNHLLYFDAAFRSGMIKSHHQ 195
           G  G+       YQIPM           IGNV  RG P         +AFRS ++     
Sbjct: 139 GGGGSC------YQIPMSRPVYFLDLMGIGNV-GRGQPPP-----VTSAFRSPVV----- 198

Query: 196 NQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPP 235
                    H A         + G QSDSDSSSVVD  G  ++ R   LD DLN PPP
Sbjct: 199 ---------HVATK------MACGAQSDSDSSSVVDFEG-GMEKRSQLLDLDLNLPPP 218

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BLAST of MS010881 vs. ExPASy Swiss-Prot
Match: Q9FE67 (Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana OX=3702 GN=ERF9 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 9.1e-29
Identity = 73/122 (59.84%), Postives = 88/122 (72.13%), Query Frame = 0


Query: 1   MAPRE---KAVAV-KGNGGSV-------KEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLG 60
           MAPR+   +++AV +G GG         KEVHFRGVRKRPWGRYAAEIRDP KK+RVWLG
Sbjct: 1   MAPRQANGRSIAVSEGGGGKTMTMTTMRKEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLG 60

Query: 61  TFDTAEEAARAYDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSS 112
           TFDTAEEAARAYDTAAREFRG+KAKTNFPLP +     +  +N+        + T  +++
Sbjct: 61  TFDTAEEAARAYDTAAREFRGSKAKTNFPLPGE-----STTVNDGGENDSYVNRTTVTTA 117

[Close]

BLAST of MS010881 vs. ExPASy Swiss-Prot
Match: Q9C5I3 (Ethylene-responsive transcription factor 11 OS=Arabidopsis thaliana OX=3702 GN=ERF11 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.5e-28
Identity = 82/142 (57.75%), Postives = 90/142 (63.38%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAP  K  AVK N G+   V +RGVRKRPWGRYAAEIRDP KKSRVWLGTFDT EEAARA
Sbjct: 1   MAPTVKTAAVKTNEGN--GVRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120
           YD  A EFRGAKAKTNFP       N+N    ++  QS SQSSTVESS            
Sbjct: 61  YDKRAIEFRGAKAKTNFP-----CYNINAHCLSL-TQSLSQSSTVESSFP---------- 120

Query: 121 LNLNLGHGIAGAISIPFQRYQI 143
            NLNLG     +   PF + Q+
Sbjct: 121 -NLNLGSDSVSS-RFPFPKIQV 122

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BLAST of MS010881 vs. ExPASy TrEMBL
Match: A0A6J1CKD6 (ethylene-responsive transcription factor 4-like OS=Momordica charantia OX=3673 GN=LOC111012250 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.3e-130
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120
           YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP
Sbjct: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120

Query: 121 LNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRL 180
           LNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRL
Sbjct: 121 LNLNLGHGIAGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRL 180

Query: 181 PFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA 238
           PFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA
Sbjct: 181 PFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPPETA 237

[Close]

BLAST of MS010881 vs. ExPASy TrEMBL
Match: A0A6J1EIT5 (ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111433009 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.6e-108
Identity = 209/249 (83.94%), Postives = 220/249 (88.35%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKANVGNVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNI-NNQSPSQSSTVESSSREQALMVDSS 120
           YD AAREFRG KAKTNFPLPS DLLNLNNKINNI NNQSPSQSSTVESSSREQ LMVDSS
Sbjct: 61  YDKAAREFRGVKAKTNFPLPSDDLLNLNNKINNINNNQSPSQSSTVESSSREQPLMVDSS 120

Query: 121 PLNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSH 180
           PLNLNL HG+     AGA++ PFQRYQIPMIG VF+RGIP SNH+LYFDAA R+GMIK+H
Sbjct: 121 PLNLNLAHGMPGLTAAGAVTFPFQRYQIPMIGEVFSRGIPPSNHVLYFDAALRAGMIKTH 180

Query: 181 -HQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRG-----SGLDFD 238
            H NQRL FDHHHEAVSDFRR++A GGVQSDSDSSSVVDMNGQDLKPR       GLD D
Sbjct: 181 PHPNQRLRFDHHHEAVSDFRREYAGGGVQSDSDSSSVVDMNGQDLKPRSGGGGVGGLDLD 240

[Close]

BLAST of MS010881 vs. ExPASy TrEMBL
Match: A0A6J1ILY8 (ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111478617 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.0e-107
Identity = 206/247 (83.40%), Postives = 218/247 (88.26%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKANVGNVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120
           YD AAREFRG KAKTNFP+PS DLLNLNNKIN  NNQSPSQSSTVESSSREQ LMVDSSP
Sbjct: 61  YDKAAREFRGVKAKTNFPMPSYDLLNLNNKINISNNQSPSQSSTVESSSREQPLMVDSSP 120

Query: 121 LNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSH- 180
           LNLNLGHG+      GA++ PFQRYQIPMIG VF+RGIP SNH+LYFDAA R+GMIK+H 
Sbjct: 121 LNLNLGHGMPGLTSTGAVTFPFQRYQIPMIGEVFSRGIPPSNHVLYFDAALRAGMIKTHP 180

Query: 181 HQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRG----SGLDFDLN 238
           H NQRL FDHH EAVSDFRR++A GGVQSDSDSSSVVDMNGQDLKPRG     GLD DLN
Sbjct: 181 HPNQRLRFDHHPEAVSDFRREYAGGGVQSDSDSSSVVDMNGQDLKPRGGVGVGGLDLDLN 240

[Close]

BLAST of MS010881 vs. ExPASy TrEMBL
Match: A0A5A7UGN5 (Ethylene-responsive transcription factor 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007640 PE=4 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 7.6e-103
Identity = 208/247 (84.21%), Postives = 213/247 (86.23%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDP KKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKPNVGNVKEVHFRGVRKRPWGRYAAEIRDPSKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQD-LLNLNNKINNI-NNQSPSQSSTVESSSREQALMVDS 120
           YD AAREFRG KAKTNFPLPS D LLNLNNKINNI NNQSPSQSSTVESSSREQALMVDS
Sbjct: 61  YDNAAREFRGVKAKTNFPLPSDDKLLNLNNKINNINNNQSPSQSSTVESSSREQALMVDS 120

Query: 121 SPLNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKS 180
           SPL+LNLGHGI     AG IS PFQRYQIPMIG VF RGIP SNH+LYFDAA R+GMI S
Sbjct: 121 SPLDLNLGHGIGGLTTAGPISFPFQRYQIPMIGEVFTRGIPPSNHVLYFDAALRTGMINS 180

Query: 181 HHQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSG---LDFDLN 238
            H NQRL FD   EAVSDFRRDFA  GVQSDSDSSSVVDMNGQDLKPRG     LD DLN
Sbjct: 181 -HPNQRLHFDRIREAVSDFRRDFAGSGVQSDSDSSSVVDMNGQDLKPRGGSGGRLDLDLN 240

[Close]

BLAST of MS010881 vs. ExPASy TrEMBL
Match: A0A1S3B0A2 (ethylene-responsive transcription factor 4-like OS=Cucumis melo OX=3656 GN=LOC103484503 PE=4 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 7.6e-103
Identity = 208/247 (84.21%), Postives = 213/247 (86.23%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAPREKAVAVK N G+VKEVHFRGVRKRPWGRYAAEIRDP KKSRVWLGTFDTAEEAARA
Sbjct: 1   MAPREKAVAVKPNVGNVKEVHFRGVRKRPWGRYAAEIRDPSKKSRVWLGTFDTAEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQD-LLNLNNKINNI-NNQSPSQSSTVESSSREQALMVDS 120
           YD AAREFRG KAKTNFPLPS D LLNLNNKINNI NNQSPSQSSTVESSSREQALMVDS
Sbjct: 61  YDNAAREFRGVKAKTNFPLPSDDKLLNLNNKINNINNNQSPSQSSTVESSSREQALMVDS 120

Query: 121 SPLNLNLGHGI-----AGAISIPFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKS 180
           SPL+LNLGHGI     AG IS PFQRYQIPMIG VF RGIP SNH+LYFDAA R+GMI S
Sbjct: 121 SPLDLNLGHGIGGLTTAGPISFPFQRYQIPMIGEVFTRGIPPSNHVLYFDAALRTGMINS 180

Query: 181 HHQNQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSG---LDFDLN 238
            H NQRL FD   EAVSDFRRDFA  GVQSDSDSSSVVDMNGQDLKPRG     LD DLN
Sbjct: 181 -HPNQRLHFDRIREAVSDFRRDFAGSGVQSDSDSSSVVDMNGQDLKPRGGSGGRLDLDLN 240

[Close]

BLAST of MS010881 vs. TAIR 10
Match: AT3G15210.1 (ethylene responsive element binding factor 4 )

HSP 1 Score: 154.1 bits (388), Expect = 1.4e-37
Identity = 115/238 (48.32%), Postives = 137/238 (57.56%), Query Frame = 0


Query: 16  SVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFRGAKAKT 75
           + KE+ +RGVRKRPWGRYAAEIRDP KK+RVWLGTFDTAEEAARAYDTAAR+FRGAKAKT
Sbjct: 19  NAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKAKT 78

Query: 76  NFPLPSQDLLNLNN-KINNINNQSPSQSSTVESSSREQALMVDSS-------PLNLNLGH 135
           NFP      L L++ K+     +SPSQSST++ +S    L+V S+        L L+LG 
Sbjct: 79  NFP----TFLELSDQKVPTGFARSPSQSSTLDCAS-PPTLVVPSATAGNVPPQLELSLGG 138

Query: 136 GIAGAISIPFQRYQIPM-----------IGNVFARGIPASNHLLYFDAAFRSGMIKSHHQ 195
           G  G+       YQIPM           IGNV  RG P         +AFRS ++     
Sbjct: 139 GGGGSC------YQIPMSRPVYFLDLMGIGNV-GRGQPPP-----VTSAFRSPVV----- 198

Query: 196 NQRLPFDHHHEAVSDFRRDFASGGVQSDSDSSSVVDMNGQDLKPRGSGLDFDLNFPPP 235
                    H A         + G QSDSDSSSVVD  G  ++ R   LD DLN PPP
Sbjct: 199 ---------HVATK------MACGAQSDSDSSSVVDFEG-GMEKRSQLLDLDLNLPPP 218

[Close]

BLAST of MS010881 vs. TAIR 10
Match: AT5G44210.1 (erf domain protein 9 )

HSP 1 Score: 128.6 bits (322), Expect = 6.5e-30
Identity = 73/122 (59.84%), Postives = 88/122 (72.13%), Query Frame = 0


Query: 1   MAPRE---KAVAV-KGNGGSV-------KEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLG 60
           MAPR+   +++AV +G GG         KEVHFRGVRKRPWGRYAAEIRDP KK+RVWLG
Sbjct: 1   MAPRQANGRSIAVSEGGGGKTMTMTTMRKEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLG 60

Query: 61  TFDTAEEAARAYDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSS 112
           TFDTAEEAARAYDTAAREFRG+KAKTNFPLP +     +  +N+        + T  +++
Sbjct: 61  TFDTAEEAARAYDTAAREFRGSKAKTNFPLPGE-----STTVNDGGENDSYVNRTTVTTA 117

[Close]

BLAST of MS010881 vs. TAIR 10
Match: AT1G53170.1 (ethylene response factor 8 )

HSP 1 Score: 126.3 bits (316), Expect = 3.2e-29
Identity = 92/224 (41.07%), Postives = 115/224 (51.34%), Query Frame = 0


Query: 16  SVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFRGAKAKT 75
           + KE+ +RGVRKRPWGRYAAEIRDP KK+RVWLGTFDTA++AARAYD AAR+FRG KAKT
Sbjct: 25  AAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAAARDFRGVKAKT 84

Query: 76  NFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSPLNLNLGHGIAGAISI 135
           NF +              I   SP+QSSTV  S      +   +P +L L  G  GA   
Sbjct: 85  NFGV--------------IVGSSPTQSSTVVDSPTAARFI---TPPHLELSLGGGGAC-- 144

Query: 136 PFQRYQIPMIGNVFARGIPASNHLLYFDAAFRSGMIKSHHQNQRLPFDHHHEAVSDFRRD 195
              R +IP++  V+           Y++ A    M                         
Sbjct: 145 ---RRKIPLVHPVY-----------YYNMATYPKM------------------------- 183

Query: 196 FASGGVQSDSDSSSVVDMNGQDLKPRGSG-----LDFDLNFPPP 235
             + GVQS+S++SSVVD  G      G+G     LD DLN  PP
Sbjct: 205 -TTCGVQSESETSSVVDFEG------GAGKISPPLDLDLNLAPP 183

[Close]

BLAST of MS010881 vs. TAIR 10
Match: AT1G28370.1 (ERF domain protein 11 )

HSP 1 Score: 126.3 bits (316), Expect = 3.2e-29
Identity = 82/142 (57.75%), Postives = 90/142 (63.38%), Query Frame = 0


Query: 1   MAPREKAVAVKGNGGSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARA 60
           MAP  K  AVK N G+   V +RGVRKRPWGRYAAEIRDP KKSRVWLGTFDT EEAARA
Sbjct: 1   MAPTVKTAAVKTNEGN--GVRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARA 60

Query: 61  YDTAAREFRGAKAKTNFPLPSQDLLNLNNKINNINNQSPSQSSTVESSSREQALMVDSSP 120
           YD  A EFRGAKAKTNFP       N+N    ++  QS SQSSTVESS            
Sbjct: 61  YDKRAIEFRGAKAKTNFP-----CYNINAHCLSL-TQSLSQSSTVESSFP---------- 120

Query: 121 LNLNLGHGIAGAISIPFQRYQI 143
            NLNLG     +   PF + Q+
Sbjct: 121 -NLNLGSDSVSS-RFPFPKIQV 122

[Close]

BLAST of MS010881 vs. TAIR 10
Match: AT1G50640.1 (ethylene responsive element binding factor 3 )

HSP 1 Score: 123.2 bits (308), Expect = 2.7e-28
Identity = 61/87 (70.11%), Postives = 70/87 (80.46%), Query Frame = 0


Query: 15  GSVKEVHFRGVRKRPWGRYAAEIRDPCKKSRVWLGTFDTAEEAARAYDTAAREFRGAKAK 74
           GSVKE+ FRGVRKRPWGR+AAEIRDP KK+RVWLGTFD+AEEAARAYD+AAR  RG KAK
Sbjct: 21  GSVKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTFDSAEEAARAYDSAARNLRGPKAK 80

Query: 75  TNFPLPSQDLLNLNNKINNINNQSPSQ 102
           TNFP+ S      N + N I NQ+ +Q
Sbjct: 81  TNFPIDSSSPPPPNLRFNQIRNQNQNQ 107

The following BLAST results are available for this feature:

BLAST of MS010881 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142019.1	8.8e-130	100.00	ethylene-responsive transcription factor 4-like [Momordica charantia]	[more]
KAG6604462.1	2.5e-108	84.27	Ethylene-responsive transcription factor 4, partial [Cucurbita argyrosperma subs...	[more]
XP_023545040.1	2.5e-108	84.27	ethylene-responsive transcription factor 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022925660.1	3.3e-108	83.94	ethylene-responsive transcription factor 4-like [Cucurbita moschata] >KAG7034605...	[more]
XP_022978752.1	2.1e-107	83.40	ethylene-responsive transcription factor 4-like [Cucurbita maxima]	[more]

BLAST of MS010881 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW49	6.3e-38	46.44	Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ER...	[more]
Q40477	3.1e-37	46.86	Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 ...	[more]
O80340	2.0e-36	48.32	Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9FE67	9.1e-29	59.84	Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana OX=3702 GN=ER...	[more]
Q9C5I3	4.5e-28	57.75	Ethylene-responsive transcription factor 11 OS=Arabidopsis thaliana OX=3702 GN=E...	[more]

BLAST of MS010881 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKD6	4.3e-130	100.00	ethylene-responsive transcription factor 4-like OS=Momordica charantia OX=3673 G...	[more]
A0A6J1EIT5	1.6e-108	83.94	ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1ILY8	1.0e-107	83.40	ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A5A7UGN5	7.6e-103	84.21	Ethylene-responsive transcription factor 4-like OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3B0A2	7.6e-103	84.21	ethylene-responsive transcription factor 4-like OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of MS010881 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15210.1	1.4e-37	48.32	ethylene responsive element binding factor 4	[more]
AT5G44210.1	6.5e-30	59.84	erf domain protein 9	[more]
AT1G53170.1	3.2e-29	41.07	ethylene response factor 8	[more]
AT1G28370.1	3.2e-29	57.75	ERF domain protein 11	[more]
AT1G50640.1	2.7e-28	70.11	ethylene responsive element binding factor 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 22..33 score: 60.91 coord: 44..60 score: 75.4
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 21..84 e-value: 1.2E-38 score: 144.3
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 21..70 e-value: 3.5E-14 score: 52.8
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 21..78 score: 23.709442
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 21..78 e-value: 2.90983E-31 score: 107.743
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 20..79 e-value: 1.6E-28 score: 100.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 197..237
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 200..214
None	No IPR available	PANTHER	PTHR31677:SF151	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 4-LIKE	coord: 1..226
None	No IPR available	PANTHER	PTHR31677	AP2 DOMAIN CLASS TRANSCRIPTION FACTOR	coord: 1..226
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 21..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010881.1	MS010881.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity