MS010878 (gene) Bitter gourd (TR) v1

BLAST of MS010878 vs. NCBI nr
Match: XP_023543675.1 (putative E3 ubiquitin-protein ligase RING1a isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.1 bits (1224), Expect = 2.5e-130
Identity = 265/371 (71.43%), Postives = 294/371 (79.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 24  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 83

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR RK+ +
Sbjct: 84  YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKSFQ 143

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSAT---WADG 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT    ADG
Sbjct: 144 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGADG 203

Query: 181 GSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLL 240
           GSNENDYEVNKE VG S+G+V SSE+L+WGKGG+RSHTRYGGINGG GKISR NR TKL 
Sbjct: 204 GSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITKLS 263

Query: 241 DYVQNSENISEEE------------------------------------YVALRTALPVD 300
           DYV+NSENISEEE                                     VAL TAL VD
Sbjct: 264 DYVRNSENISEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALETALSVD 323

Query: 301 LVEMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLEL 332
           ++EMY+VKELQMK+NPSTS T +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE 
Sbjct: 324 VIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LEP 383

BLAST of MS010878 vs. NCBI nr
Match: XP_022925653.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita moschata])

HSP 1 Score: 476.1 bits (1224), Expect = 2.5e-130
Identity = 265/371 (71.43%), Postives = 294/371 (79.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 66  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 125

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR RK+ +
Sbjct: 126 YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKSFQ 185

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSAT---WADG 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT    ADG
Sbjct: 186 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGADG 245

Query: 181 GSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLL 240
           GSNENDYEVNKE VG S+G+V SSE+L+WGKGG+RSHTRYGGINGG GKISR NR TKL 
Sbjct: 246 GSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITKLS 305

Query: 241 DYVQNSENISEEE------------------------------------YVALRTALPVD 300
           DYV+NSENISEEE                                     VAL TAL VD
Sbjct: 306 DYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALSVD 365

Query: 301 LVEMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLEL 332
           ++EMY+VKELQMK+NPSTS T +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE 
Sbjct: 366 VIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LEP 425

BLAST of MS010878 vs. NCBI nr
Match: XP_023543673.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.1 bits (1224), Expect = 2.5e-130
Identity = 265/371 (71.43%), Postives = 294/371 (79.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 66  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 125

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR RK+ +
Sbjct: 126 YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKSFQ 185

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSAT---WADG 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT    ADG
Sbjct: 186 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGADG 245

Query: 181 GSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLL 240
           GSNENDYEVNKE VG S+G+V SSE+L+WGKGG+RSHTRYGGINGG GKISR NR TKL 
Sbjct: 246 GSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITKLS 305

Query: 241 DYVQNSENISEEE------------------------------------YVALRTALPVD 300
           DYV+NSENISEEE                                     VAL TAL VD
Sbjct: 306 DYVRNSENISEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALETALSVD 365

Query: 301 LVEMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLEL 332
           ++EMY+VKELQMK+NPSTS T +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE 
Sbjct: 366 VIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LEP 425

BLAST of MS010878 vs. NCBI nr
Match: XP_023517421.1 (putative E3 ubiquitin-protein ligase RING1a isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 475.7 bits (1223), Expect = 3.2e-130
Identity = 261/353 (73.94%), Postives = 284/353 (80.45%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL
Sbjct: 21  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 80

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQ+SIAQ LQRQTEALGRKRPK SR LSSR RKN +
Sbjct: 81  YPDIDKYEEEELAFQEEEKARNKQIQASIAQILQRQTEALGRKRPKPSRRLSSRGRKNFQ 140

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSSAT---WADGG 180
           N+ ++ G+D+NEDEND + SKNSSS DER+D +PKR R G PVRFS SSSAT    ADGG
Sbjct: 141 NHIDSLGFDENEDENDYEVSKNSSSADERMDTRPKRSRRGCPVRFSLSSSATGADGADGG 200

Query: 181 SNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLLD 240
            NENDYEVNKESVG S G+VGSSERL+WGKGG+RSHTRYGG NGG GKISR NR TKL D
Sbjct: 201 GNENDYEVNKESVGASTGLVGSSERLSWGKGGIRSHTRYGGTNGGTGKISRTNRHTKLSD 260

Query: 241 YVQNSENISEE-----------------------------------EYVALRTALPVDLV 300
           YV+N+ENISEE                                   +YVAL TAL VD+V
Sbjct: 261 YVRNTENISEELDVHLMLVSMDENRIPALQRPFLCCRPSVTIGHLSQYVALETALSVDVV 320

Query: 301 EMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLV 316
           EMYMVKELQMK+NPSTS TLMDP KDS+QILNEQETLS+ KLKTH L+CGYL+
Sbjct: 321 EMYMVKELQMKLNPSTSRTLMDPCKDSVQILNEQETLSMVKLKTHCLACGYLL 373

BLAST of MS010878 vs. NCBI nr
Match: XP_023517419.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 475.7 bits (1223), Expect = 3.2e-130
Identity = 261/353 (73.94%), Postives = 284/353 (80.45%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL
Sbjct: 66  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 125

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQ+SIAQ LQRQTEALGRKRPK SR LSSR RKN +
Sbjct: 126 YPDIDKYEEEELAFQEEEKARNKQIQASIAQILQRQTEALGRKRPKPSRRLSSRGRKNFQ 185

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSSAT---WADGG 180
           N+ ++ G+D+NEDEND + SKNSSS DER+D +PKR R G PVRFS SSSAT    ADGG
Sbjct: 186 NHIDSLGFDENEDENDYEVSKNSSSADERMDTRPKRSRRGCPVRFSLSSSATGADGADGG 245

Query: 181 SNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLLD 240
            NENDYEVNKESVG S G+VGSSERL+WGKGG+RSHTRYGG NGG GKISR NR TKL D
Sbjct: 246 GNENDYEVNKESVGASTGLVGSSERLSWGKGGIRSHTRYGGTNGGTGKISRTNRHTKLSD 305

Query: 241 YVQNSENISEE-----------------------------------EYVALRTALPVDLV 300
           YV+N+ENISEE                                   +YVAL TAL VD+V
Sbjct: 306 YVRNTENISEELDVHLMLVSMDENRIPALQRPFLCCRPSVTIGHLSQYVALETALSVDVV 365

Query: 301 EMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLV 316
           EMYMVKELQMK+NPSTS TLMDP KDS+QILNEQETLS+ KLKTH L+CGYL+
Sbjct: 366 EMYMVKELQMKLNPSTSRTLMDPCKDSVQILNEQETLSMVKLKTHCLACGYLL 418

BLAST of MS010878 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 4.2e-56
Identity = 157/366 (42.90%), Postives = 209/366 (57.10%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +D LIA L
Sbjct: 139 CLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAAL 198

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKR------------PKSS 120
           + +ID YEEEE+AF E+E ARNKQIQ+SIAQ  QRQ+EAL ++R            P+ +
Sbjct: 199 FTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIA 258

Query: 121 RGLSSRERKNIR-----NNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPV 180
            G  SR R+N R     N SE    DDN+D N+    K+SSS +   + + K+ R     
Sbjct: 259 SG--SRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTS 318

Query: 181 RFSQ---SSSATWADGGSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYG-GI 240
           R +Q   SS A   +G   +ND EV +++ G S G+V + E LAWG+GG RS+TR+G   
Sbjct: 319 RSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNT 378

Query: 241 NGGNGKISRINRPTKLLDYVQNSENISE-------------------------------- 300
           +GG+ K  R  R  KL++Y+++S + S                                 
Sbjct: 379 SGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLL 438

Query: 301 ----EEYVALRTALPVDLVEMYMVK-----ELQMKVNPSTSATLMDPSKDSMQILNEQET 305
                E+VAL+  L  + VE+ + +     +  ++  P+ +      SKD M  L + ET
Sbjct: 439 VKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPASAAASKDEMLSLEDNET 498

BLAST of MS010878 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 4.1e-51
Identity = 148/355 (41.69%), Postives = 207/355 (58.31%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D LIA L
Sbjct: 106 CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAAL 165

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKR---------PKSSR-G 120
           + +IDK+EEEE+ F+++++ARNKQIQ+SIAQ  QRQ++AL +++          +S R G
Sbjct: 166 FKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRRSG 225

Query: 121 LSSRERKNIRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSS 180
             SR R+N RN  ++   + N+D++ N   K+SSS DE  +R+ K+     P   + +++
Sbjct: 226 GGSRRRRNCRNIEQDTS-EANDDDDQNKRGKDSSS-DEPCERQRKKRSATQPSSSNANNN 285

Query: 181 ATWADGGSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRIN 240
              A  G+ +      ++S   S  +V +SE +AWG+GG RS+TR G  N   G IS+ N
Sbjct: 286 DNCAGNGTEQTH---QRDSRVISPVLVWNSELIAWGRGGTRSNTRQG--NNNQGAISKRN 345

Query: 241 -RPTKLLDYVQNSENIS--------------------EEEYVALRTALPVDLVEMYMVKE 300
            R  +L++Y+ + E  S                     E Y+  R  L V  +  Y+ + 
Sbjct: 346 ARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARH 405

Query: 301 LQMKVNPSTSATLMD--------PSKDSMQILNEQETLSIAKLKTHSLSC-GYLV 316
           L++K          D         S + MQ L + ET  +AKLK   +S  GY++
Sbjct: 406 LKLKAEEVELLVSKDGDTVIGNKTSTEKMQSLQDDET--VAKLKVDCISSNGYMI 451

BLAST of MS010878 vs. ExPASy Swiss-Prot
Match: Q6MGB6 (E3 ubiquitin-protein ligase RING1 OS=Rattus norvegicus OX=10116 GN=Ring1 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 89/287 (31.01%), Postives = 127/287 (44.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C  +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+D LI+ +
Sbjct: 51  CLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKI 110

Query: 61  YPDIDKYE-EEEMAFQEEEKARNKQ-IQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKN 120
           YP  ++YE  ++       +  N+Q + SSI + L+ Q     ++  +   G  S +   
Sbjct: 111 YPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPMPG--SDQTTT 170

Query: 121 IRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSSATWADGGS 180
           +      PG    E E D ++  + S+ D      PKRPR GG              GGS
Sbjct: 171 MSGGEGEPG----EGEGDGEDISSDSAPDSAPGPAPKRPRGGGA-------------GGS 230

Query: 181 NENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGG---------------- 240
                     SVGT  G  G +     G G   S  R G + GG                
Sbjct: 231 ----------SVGTGGGAAGGA---CGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEP 290

Query: 241 NGKISRINRPTKLL---------DYVQNSENISEE---EYVALRTAL 258
            G+I  + RP  LL          YV+ + N + +   +Y+ALR AL
Sbjct: 291 GGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIAL 305

BLAST of MS010878 vs. ExPASy Swiss-Prot
Match: Q5TJF3 (E3 ubiquitin-protein ligase RING1 OS=Canis lupus familiaris OX=9615 GN=RING1 PE=3 SV=2)

HSP 1 Score: 84.0 bits (206), Expect = 3.6e-15
Identity = 89/287 (31.01%), Postives = 127/287 (44.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C  +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+D LI+ +
Sbjct: 51  CLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKI 110

Query: 61  YPDIDKYE-EEEMAFQEEEKARNKQ-IQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKN 120
           YP  ++YE  ++       +  N+Q + SSI + L+ Q     ++  +   G  S +   
Sbjct: 111 YPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPMPG--SDQTTT 170

Query: 121 IRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSSATWADGGS 180
           +      PG    E E D ++  + S+ D      PKRPR GG              GGS
Sbjct: 171 MSGGEGEPG----EGEGDGEDVSSDSAPDSAPGPAPKRPRGGGA-------------GGS 230

Query: 181 NENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGG---------------- 240
                     SVGT  G  G    +  G G   S  R G + GG                
Sbjct: 231 ----------SVGTGGGGAGG---VGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEP 290

Query: 241 NGKISRINRPTKLL---------DYVQNSENISEE---EYVALRTAL 258
            G+I  + RP  LL          YV+ + N + +   +Y+ALR AL
Sbjct: 291 GGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIAL 305

BLAST of MS010878 vs. ExPASy Swiss-Prot
Match: Q06587 (E3 ubiquitin-protein ligase RING1 OS=Homo sapiens OX=9606 GN=RING1 PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 4.7e-15
Identity = 89/287 (31.01%), Postives = 127/287 (44.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C  +++ T T  ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+D LI+ +
Sbjct: 51  CLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKI 110

Query: 61  YPDIDKYE-EEEMAFQEEEKARNKQ-IQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKN 120
           YP  ++YE  ++       +  N+Q + SSI + L+ Q     ++  +   G  S +   
Sbjct: 111 YPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPG--SDQTTT 170

Query: 121 IRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSSATWADGGS 180
           +      PG    E E D ++  + S+ D      PKRPR GG              GGS
Sbjct: 171 MSGGEGEPG----EGEGDGEDVSSDSAPDSAPGPAPKRPRGGGA-------------GGS 230

Query: 181 NENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGG---------------- 240
                     SVGT  G  G    +  G G   S  R G + GG                
Sbjct: 231 ----------SVGTGGGGTGG---VGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEP 290

Query: 241 NGKISRINRPTKLL---------DYVQNSENISEE---EYVALRTAL 258
            G+I  + RP  LL          YV+ + N + +   +Y+ALR AL
Sbjct: 291 GGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIAL 305

BLAST of MS010878 vs. ExPASy TrEMBL
Match: A0A6J1EFU8 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.2e-130
Identity = 265/371 (71.43%), Postives = 294/371 (79.25%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 66  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 125

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR RK+ +
Sbjct: 126 YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKSFQ 185

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSAT---WADG 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT    ADG
Sbjct: 186 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGADG 245

Query: 181 GSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLL 240
           GSNENDYEVNKE VG S+G+V SSE+L+WGKGG+RSHTRYGGINGG GKISR NR TKL 
Sbjct: 246 GSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITKLS 305

Query: 241 DYVQNSENISEEE------------------------------------YVALRTALPVD 300
           DYV+NSENISEEE                                     VAL TAL VD
Sbjct: 306 DYVRNSENISEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALSVD 365

Query: 301 LVEMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLEL 332
           ++EMY+VKELQMK+NPSTS T +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE 
Sbjct: 366 VIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LEP 425

BLAST of MS010878 vs. ExPASy TrEMBL
Match: A0A6J1EIS8 (putative E3 ubiquitin-protein ligase RING1a isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433005 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.6e-130
Identity = 265/372 (71.24%), Postives = 294/372 (79.03%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 24  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 83

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR RK+ +
Sbjct: 84  YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGRKSFQ 143

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSAT---WADG 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT    ADG
Sbjct: 144 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGADG 203

Query: 181 GSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLL 240
           GSNENDYEVNKE VG S+G+V SSE+L+WGKGG+RSHTRYGGINGG GKISR NR TKL 
Sbjct: 204 GSNENDYEVNKERVGASMGLVSSSEKLSWGKGGIRSHTRYGGINGGGGKISRNNRITKLS 263

Query: 241 DYVQNSENISEEE-------------------------------------YVALRTALPV 300
           DYV+NSENISEEE                                      VAL TAL V
Sbjct: 264 DYVRNSENISEEEELDVQLMLVSMDKTRIPALRSSYICCRPSVTIGHLRQLVALETALSV 323

Query: 301 DLVEMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLE 332
           D++EMY+VKELQMK+NPSTS T +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE
Sbjct: 324 DVIEMYVVKELQMKINPSTSGTFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LE 383

BLAST of MS010878 vs. ExPASy TrEMBL
Match: A0A6J1IQZ1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478584 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 8.6e-129
Identity = 260/369 (70.46%), Postives = 290/369 (78.59%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 66  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 125

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR +K+ +
Sbjct: 126 YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGQKSFQ 185

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSATWADGGSN 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT ADGG N
Sbjct: 186 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGGGN 245

Query: 181 ENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLLDYV 240
           END EVNKE VG S+G+V SSE+ +WGKGG+RSHTRYGGINGG GKISR NR TKL DYV
Sbjct: 246 ENDCEVNKEKVGASMGLVSSSEKHSWGKGGIRSHTRYGGINGGGGKISRNNRITKLSDYV 305

Query: 241 QNSENISEEE-------------------------------------YVALRTALPVDLV 300
           +NSENISEEE                                      VAL TAL VD++
Sbjct: 306 RNSENISEEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALVTALSVDVI 365

Query: 301 EMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLELTV 332
           EMY+VKELQMK+NPSTS   +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE T 
Sbjct: 366 EMYVVKELQMKINPSTSGAFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LEPTA 425

BLAST of MS010878 vs. ExPASy TrEMBL
Match: A0A6J1ILU9 (putative E3 ubiquitin-protein ligase RING1a isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111478584 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 8.6e-129
Identity = 260/369 (70.46%), Postives = 290/369 (78.59%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPN+DTLIAVL
Sbjct: 24  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNFDTLIAVL 83

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYEEEE+AFQEEEKARNKQIQSSIAQTL+RQTEALGRKR K  R LSSR +K+ +
Sbjct: 84  YPDIDKYEEEELAFQEEEKARNKQIQSSIAQTLKRQTEALGRKRSKPLRRLSSRGQKSFQ 143

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKP-KRPRIGGPVRFSQSSSATWADGGSN 180
           N+ E+  +D+NEDEND D SKNSSS DER+D +P KR R GGPVRFSQSSSAT ADGG N
Sbjct: 144 NHIESLSFDENEDENDYDASKNSSSADERMDTRPNKRSRRGGPVRFSQSSSATGADGGGN 203

Query: 181 ENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLLDYV 240
           END EVNKE VG S+G+V SSE+ +WGKGG+RSHTRYGGINGG GKISR NR TKL DYV
Sbjct: 204 ENDCEVNKEKVGASMGLVSSSEKHSWGKGGIRSHTRYGGINGGGGKISRNNRITKLSDYV 263

Query: 241 QNSENISEEE-------------------------------------YVALRTALPVDLV 300
           +NSENISEEE                                      VAL TAL VD++
Sbjct: 264 RNSENISEEEELDVHLMLVSMDKTRIPALRSPYICCRPSVTIGHLRQLVALVTALSVDVI 323

Query: 301 EMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLVCFLELTV 332
           EMY+VKELQMK+NPSTS   +DP K+S QILN+QETLS+ KLKTH L+CGYLVC LE T 
Sbjct: 324 EMYVVKELQMKINPSTSGAFLDPCKESAQILNQQETLSMDKLKTHRLACGYLVC-LEPTA 383

BLAST of MS010878 vs. ExPASy TrEMBL
Match: A0A6J1EI61 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433615 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 8.6e-129
Identity = 258/353 (73.09%), Postives = 282/353 (79.89%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLI+VL
Sbjct: 21  CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLISVL 80

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKRPKSSRGLSSRERKNIR 120
           YPDIDKYE EE+AFQEEEKARNKQIQ+SIAQ LQRQTEALGRKRPK SR LSSR RKN +
Sbjct: 81  YPDIDKYEAEELAFQEEEKARNKQIQASIAQILQRQTEALGRKRPKPSRRLSSRGRKNFQ 140

Query: 121 NNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSSAT---WADGG 180
           N+ E+ G+D+NEDEND D SKNSSS DER+D + KR R G PVRFS SSSAT    ADGG
Sbjct: 141 NHIESLGFDENEDENDYDVSKNSSSADERMDTRTKRSRRGCPVRFSLSSSATGADGADGG 200

Query: 181 SNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRINRPTKLLD 240
            NENDYEVNKES+G S G+VGSSERL+WGKGG+RSHTRYGG NGG GKISR NR TKL D
Sbjct: 201 GNENDYEVNKESIGASTGLVGSSERLSWGKGGIRSHTRYGGTNGGTGKISRTNRHTKLSD 260

Query: 241 YVQNSENISEE-----------------------------------EYVALRTALPVDLV 300
           YV+N+ENISEE                                   +YVAL TAL VD+V
Sbjct: 261 YVRNAENISEELDVHLMLVSMDENRIPALQRPFLCCRPSVTIGHLSQYVALETALTVDVV 320

Query: 301 EMYMVKELQMKVNPSTSATLMDPSKDSMQILNEQETLSIAKLKTHSLSCGYLV 316
           EMYMVKELQMK+NPS+S TLMDP KDS+QILNEQETLS+ KLKTH L+CGYL+
Sbjct: 321 EMYMVKELQMKLNPSSSRTLMDPCKDSVQILNEQETLSMVKLKTHCLACGYLL 373

BLAST of MS010878 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 219.9 bits (559), Expect = 3.0e-57
Identity = 157/366 (42.90%), Postives = 209/366 (57.10%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GII+KTRTVMECLHRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +D LIA L
Sbjct: 139 CLGIIKKTRTVMECLHRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAAL 198

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKR------------PKSS 120
           + +ID YEEEE+AF E+E ARNKQIQ+SIAQ  QRQ+EAL ++R            P+ +
Sbjct: 199 FTNIDSYEEEELAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIA 258

Query: 121 RGLSSRERKNIR-----NNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPV 180
            G  SR R+N R     N SE    DDN+D N+    K+SSS +   + + K+ R     
Sbjct: 259 SG--SRRRRNSRNMEQQNASEAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTS 318

Query: 181 RFSQ---SSSATWADGGSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYG-GI 240
           R +Q   SS A   +G   +ND EV +++ G S G+V + E LAWG+GG RS+TR+G   
Sbjct: 319 RSTQHPSSSGANKNNGNCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNT 378

Query: 241 NGGNGKISRINRPTKLLDYVQNSENISE-------------------------------- 300
           +GG+ K  R  R  KL++Y+++S + S                                 
Sbjct: 379 SGGSSKSVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLL 438

Query: 301 ----EEYVALRTALPVDLVEMYMVK-----ELQMKVNPSTSATLMDPSKDSMQILNEQET 305
                E+VAL+  L  + VE+ + +     +  ++  P+ +      SKD M  L + ET
Sbjct: 439 VKQLREFVALQIHLKTEEVELLVTRRRVGEDAAIENLPAVTPASAAASKDEMLSLEDNET 498

BLAST of MS010878 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 203.4 bits (516), Expect = 2.9e-52
Identity = 148/355 (41.69%), Postives = 207/355 (58.31%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D LIA L
Sbjct: 106 CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAAL 165

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKR---------PKSSR-G 120
           + +IDK+EEEE+ F+++++ARNKQIQ+SIAQ  QRQ++AL +++          +S R G
Sbjct: 166 FKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRRSG 225

Query: 121 LSSRERKNIRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSS 180
             SR R+N RN  ++   + N+D++ N   K+SSS DE  +R+ K+     P   + +++
Sbjct: 226 GGSRRRRNCRNIEQDTS-EANDDDDQNKRGKDSSS-DEPCERQRKKRSATQPSSSNANNN 285

Query: 181 ATWADGGSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRIN 240
              A  G+ +      ++S   S  +V +SE +AWG+GG RS+TR G  N   G IS+ N
Sbjct: 286 DNCAGNGTEQTH---QRDSRVISPVLVWNSELIAWGRGGTRSNTRQG--NNNQGAISKRN 345

Query: 241 -RPTKLLDYVQNSENIS--------------------EEEYVALRTALPVDLVEMYMVKE 300
            R  +L++Y+ + E  S                     E Y+  R  L V  +  Y+ + 
Sbjct: 346 ARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARH 405

Query: 301 LQMKVNPSTSATLMD--------PSKDSMQILNEQETLSIAKLKTHSLSC-GYLV 316
           L++K          D         S + MQ L + ET  +AKLK   +S  GY++
Sbjct: 406 LKLKAEEVELLVSKDGDTVIGNKTSTEKMQSLQDDET--VAKLKVDCISSNGYMI 451

BLAST of MS010878 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 199.1 bits (505), Expect = 5.5e-51
Identity = 148/361 (41.00%), Postives = 208/361 (57.62%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D LIA L
Sbjct: 106 CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAAL 165

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKR---------PKSSR-G 120
           + +IDK+EEEE+ F+++++ARNKQIQ+SIAQ  QRQ++AL +++          +S R G
Sbjct: 166 FKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRRSG 225

Query: 121 LSSRERKNIRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSS 180
             SR R+N RN  ++   + N+D++ N   K+SSS DE  +R+ K+     P   + +++
Sbjct: 226 GGSRRRRNCRNIEQDTS-EANDDDDQNKRGKDSSS-DEPCERQRKKRSATQPSSSNANNN 285

Query: 181 ATWADGGSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRIN 240
              A  G+ +      ++S   S  +V +SE +AWG+GG RS+TR G  N   G IS+ N
Sbjct: 286 DNCAGNGTEQTH---QRDSRVISPVLVWNSELIAWGRGGTRSNTRQG--NNNQGAISKRN 345

Query: 241 -RPTKLLDYVQNSENIS--------------------EEEYVALRTAL------PVDLVE 300
            R  +L++Y+ + E  S                     E Y+  R  L       V  + 
Sbjct: 346 ARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLP 405

Query: 301 MYMVKELQMKVNPSTSATLMD--------PSKDSMQILNEQETLSIAKLKTHSLSC-GYL 316
           +Y+ + L++K          D         S + MQ L + ET  +AKLK   +S  GY+
Sbjct: 406 LYVARHLKLKAEEVELLVSKDGDTVIGNKTSTEKMQSLQDDET--VAKLKVDCISSNGYM 457

BLAST of MS010878 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 199.1 bits (505), Expect = 5.5e-51
Identity = 148/361 (41.00%), Postives = 208/361 (57.62%), Query Frame = 0

Query: 1   CAGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDTLIAVL 60
           C GIIRKTRTVMECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+D LIA L
Sbjct: 108 CLGIIRKTRTVMECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAAL 167

Query: 61  YPDIDKYEEEEMAFQEEEKARNKQIQSSIAQTLQRQTEALGRKR---------PKSSR-G 120
           + +IDK+EEEE+ F+++++ARNKQIQ+SIAQ  QRQ++AL +++          +S R G
Sbjct: 168 FKNIDKFEEEELNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTAILSRSRRSG 227

Query: 121 LSSRERKNIRNNSENPGYDDNEDENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQSSS 180
             SR R+N RN  ++   + N+D++ N   K+SSS DE  +R+ K+     P   + +++
Sbjct: 228 GGSRRRRNCRNIEQDTS-EANDDDDQNKRGKDSSS-DEPCERQRKKRSATQPSSSNANNN 287

Query: 181 ATWADGGSNENDYEVNKESVGTSIGIVGSSERLAWGKGGMRSHTRYGGINGGNGKISRIN 240
              A  G+ +      ++S   S  +V +SE +AWG+GG RS+TR G  N   G IS+ N
Sbjct: 288 DNCAGNGTEQTH---QRDSRVISPVLVWNSELIAWGRGGTRSNTRQG--NNNQGAISKRN 347

Query: 241 -RPTKLLDYVQNSENIS--------------------EEEYVALRTAL------PVDLVE 300
            R  +L++Y+ + E  S                     E Y+  R  L       V  + 
Sbjct: 348 ARLKRLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLP 407

Query: 301 MYMVKELQMKVNPSTSATLMD--------PSKDSMQILNEQETLSIAKLKTHSLSC-GYL 316
           +Y+ + L++K          D         S + MQ L + ET  +AKLK   +S  GY+
Sbjct: 408 LYVARHLKLKAEEVELLVSKDGDTVIGNKTSTEKMQSLQDDET--VAKLKVDCISSNGYM 459

BLAST of MS010878 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 159.5 bits (402), Expect = 4.8e-39
Identity = 129/336 (38.39%), Postives = 180/336 (53.57%), Query Frame = 0

Query: 31  NNECPACRTHCASRRSLRDDPNYDTLIAVLYPDIDKYEEEEMAFQEEEKARNKQIQSSIA 90
           NNECPACR HCASRRSLRDDP +D LIA L+ +ID YEEEE+AF E+E ARNKQIQ+SIA
Sbjct: 172 NNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIA 231

Query: 91  QTLQRQTEALGRKR------------PKSSRGLSSRERKNIR-----NNSENPGYDDNED 150
           Q  QRQ+EAL ++R            P+ + G  SR R+N R     N SE    DDN+D
Sbjct: 232 QISQRQSEALVKRRSLGKEAAVLMRSPRIASG--SRRRRNSRNMEQQNASEAHEDDDNDD 291

Query: 151 ENDNDESKNSSSTDERIDRKPKRPRIGGPVRFSQ---SSSATWADGGSNENDYEVNKESV 210
            N+    K+SSS +   + + K+ R     R +Q   SS A   +G   +ND EV +++ 
Sbjct: 292 NNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNGNCADNDTEVYRDTK 351

Query: 211 GTSIGIVGSSERLAWGKGGMRSHTRYG-GINGGNGKISRINRPTKLLDYVQNSENISE-- 270
           G S G+V + E LAWG+GG RS+TR+G   +GG+ K  R  R  KL++Y+++S + S   
Sbjct: 352 GISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVDGSSVE 411

Query: 271 ----------------------------------EEYVALRTALPVDLVEMYMVK----- 305
                                              E+VAL+  L  + VE+ + +     
Sbjct: 412 LDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVTRRRVGE 471